CmaCh05G011240 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh05G011240
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protein RALF-like 33
Location	Cma_Chr05: 8819661 .. 8820020 (+)
RNA-Seq Expression	CmaCh05G011240
Synteny	CmaCh05G011240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGGATTCAATTCTCCGACCTTCCTTCTGATCTGCGCCACCGTGTTCGTGGTCTTCAGCTTCTCCTCAACCGCCGTCCTCGGAAGCCTCGGGATCCAGCACAGCGTTGCATGGATTCCTAACCAGGCGACCTGCAAGGGCTCCATAGCGGAGTGTTTCGGCGGAGAGGAGTTCGAATTTGATTCCGAAATCAGCCGCCGTATCTTGGCCACCAGCCAGTACATCAGCTACGGCGCTCTGCGGAGGAACACGGTACCTTGCTCCAGACGCGGTGCATCCTACTACAATTGCCAGCCTGGAGCTCAGGCCAATCCCTATAGCCGTGGATGCAGCGCCATTACGAGGTGCAGGAGTTGA

mRNA sequence

ATGGCAGGATTCAATTCTCCGACCTTCCTTCTGATCTGCGCCACCGTGTTCGTGGTCTTCAGCTTCTCCTCAACCGCCGTCCTCGGAAGCCTCGGGATCCAGCACAGCGTTGCATGGATTCCTAACCAGGCGACCTGCAAGGGCTCCATAGCGGAGTGTTTCGGCGGAGAGGAGTTCGAATTTGATTCCGAAATCAGCCGCCGTATCTTGGCCACCAGCCAGTACATCAGCTACGGCGCTCTGCGGAGGAACACGGTACCTTGCTCCAGACGCGGTGCATCCTACTACAATTGCCAGCCTGGAGCTCAGGCCAATCCCTATAGCCGTGGATGCAGCGCCATTACGAGGTGCAGGAGTTGA

Coding sequence (CDS)

ATGGCAGGATTCAATTCTCCGACCTTCCTTCTGATCTGCGCCACCGTGTTCGTGGTCTTCAGCTTCTCCTCAACCGCCGTCCTCGGAAGCCTCGGGATCCAGCACAGCGTTGCATGGATTCCTAACCAGGCGACCTGCAAGGGCTCCATAGCGGAGTGTTTCGGCGGAGAGGAGTTCGAATTTGATTCCGAAATCAGCCGCCGTATCTTGGCCACCAGCCAGTACATCAGCTACGGCGCTCTGCGGAGGAACACGGTACCTTGCTCCAGACGCGGTGCATCCTACTACAATTGCCAGCCTGGAGCTCAGGCCAATCCCTATAGCCGTGGATGCAGCGCCATTACGAGGTGCAGGAGTTGA

Protein sequence

MAGFNSPTFLLICATVFVVFSFSSTAVLGSLGIQHSVAWIPNQATCKGSIAECFGGEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS

Homology

[Close]

BLAST of CmaCh05G011240 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.4e-30
Identity = 72/121 (59.50%), Postives = 87/121 (71.90%), Query Frame = 0


Query: 1   MAGFNSPTFLLICATVFVVFSFSSTAVLGSLGIQHSVAWIPNQATCKGSIAEC---FGGE 60
           M G ++    +I A + V F F++         Q S  ++P ++ C G+IAEC      E
Sbjct: 1   MRGLSTKPVAIIIAILTVHFLFAAVT------SQSSGDFVPIESKCNGTIAECSLSTAEE 60

Query: 61  EFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRC 119
           EFE DSEI+RRILAT++YISYGALRRNTVPCSRRGASYYNC+ GAQANPYSRGCSAITRC
Sbjct: 61  EFEMDSEINRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRC 115

[Close]

BLAST of CmaCh05G011240 vs. ExPASy Swiss-Prot
Match: Q9SRY3 (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 3.2e-30
Identity = 72/114 (63.16%), Postives = 81/114 (71.05%), Query Frame = 0


Query: 10  LLICATVFVVFSFSSTAV-LGSLGIQHSVAWI---PNQATCKGSIAECFGGEEFEFDSEI 69
           L +  T+ VVF  SS  V  G       VAW     N + C GSIAEC G EE E DSEI
Sbjct: 7   LFLTLTILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHGSIAECIGAEEEEMDSEI 66

Query: 70  SRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS 120
           +RRILAT++YISY +L+RN+VPCSRRGASYYNCQ GAQANPYSRGCS I RCRS
Sbjct: 67  NRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of CmaCh05G011240 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 4.1e-30
Identity = 71/114 (62.28%), Postives = 82/114 (71.93%), Query Frame = 0


Query: 7   PTFLLICATVFVVFSFSSTAVLGSLGIQHSVAWIPNQATCKGSIAECFG-GEEFEFDSEI 66
           P+ L++C  V +   F S A  G  G    V    +   CKGSI EC    EEFE DSE 
Sbjct: 4   PSGLILC--VLIGAFFISMAAAGDSGAYDWVMPARSGGGCKGSIGECIAEEEEFELDSES 63

Query: 67  SRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS 120
           +RRILAT +YISYGAL++N+VPCSRRGASYYNC+PGAQANPYSRGCSAITRCRS
Sbjct: 64  NRRILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

[Close]

BLAST of CmaCh05G011240 vs. ExPASy Swiss-Prot
Match: Q9LUS7 (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 5.0e-28
Identity = 67/124 (54.03%), Postives = 84/124 (67.74%), Query Frame = 0


Query: 12  ICATVFVVFSFSSTAVLGSLGIQHSVAWIPNQATCKGSIAEC-------------FG--- 71
           I A + ++   + +  + S   + +  + P +  C+G+IAEC             +G   
Sbjct: 13  IFAILLILAVHNWSVAVSSQSTEFAGDFPPFETECRGTIAECSVSAALGDGGDLFYGGGE 72

Query: 72  -GEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAI 119
            GEEFE DSEI+RRILAT +YISYGALRRNT+PCSRRGASYYNC+ GAQANPYSRGCSAI
Sbjct: 73  MGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAI 132

[Close]

BLAST of CmaCh05G011240 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 6.6e-28
Identity = 60/74 (81.08%), Postives = 64/74 (86.49%), Query Frame = 0


Query: 46  CKGSIAECFG-GEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQA 105
           C GSIAEC    EE EFDS+ISRRILA  +YISYGA+RRN+VPCSRRGASYYNCQ GAQA
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQA 104

Query: 106 NPYSRGCSAITRCR 119
           NPYSRGCS ITRCR
Sbjct: 105 NPYSRGCSTITRCR 118

[Close]

BLAST of CmaCh05G011240 vs. TAIR 10
Match: AT4G15800.1 (ralf-like 33 )

HSP 1 Score: 133.7 bits (335), Expect = 1.0e-31
Identity = 72/121 (59.50%), Postives = 87/121 (71.90%), Query Frame = 0


Query: 1   MAGFNSPTFLLICATVFVVFSFSSTAVLGSLGIQHSVAWIPNQATCKGSIAEC---FGGE 60
           M G ++    +I A + V F F++         Q S  ++P ++ C G+IAEC      E
Sbjct: 1   MRGLSTKPVAIIIAILTVHFLFAAVT------SQSSGDFVPIESKCNGTIAECSLSTAEE 60

Query: 61  EFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRC 119
           EFE DSEI+RRILAT++YISYGALRRNTVPCSRRGASYYNC+ GAQANPYSRGCSAITRC
Sbjct: 61  EFEMDSEINRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRC 115

[Close]

BLAST of CmaCh05G011240 vs. TAIR 10
Match: AT1G02900.1 (rapid alkalinization factor 1 )

HSP 1 Score: 132.5 bits (332), Expect = 2.2e-31
Identity = 72/114 (63.16%), Postives = 81/114 (71.05%), Query Frame = 0


Query: 10  LLICATVFVVFSFSSTAV-LGSLGIQHSVAWI---PNQATCKGSIAECFGGEEFEFDSEI 69
           L +  T+ VVF  SS  V  G       VAW     N + C GSIAEC G EE E DSEI
Sbjct: 7   LFLTLTILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHGSIAECIGAEEEEMDSEI 66

Query: 70  SRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS 120
           +RRILAT++YISY +L+RN+VPCSRRGASYYNCQ GAQANPYSRGCS I RCRS
Sbjct: 67  NRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of CmaCh05G011240 vs. TAIR 10
Match: AT3G16570.1 (rapid alkalinization factor 23 )

HSP 1 Score: 125.2 bits (313), Expect = 3.6e-29
Identity = 67/124 (54.03%), Postives = 84/124 (67.74%), Query Frame = 0


Query: 12  ICATVFVVFSFSSTAVLGSLGIQHSVAWIPNQATCKGSIAEC-------------FG--- 71
           I A + ++   + +  + S   + +  + P +  C+G+IAEC             +G   
Sbjct: 13  IFAILLILAVHNWSVAVSSQSTEFAGDFPPFETECRGTIAECSVSAALGDGGDLFYGGGE 72

Query: 72  -GEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAI 119
            GEEFE DSEI+RRILAT +YISYGALRRNT+PCSRRGASYYNC+ GAQANPYSRGCSAI
Sbjct: 73  MGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAI 132

[Close]

BLAST of CmaCh05G011240 vs. TAIR 10
Match: AT3G05490.1 (ralf-like 22 )

HSP 1 Score: 124.8 bits (312), Expect = 4.7e-29
Identity = 60/74 (81.08%), Postives = 64/74 (86.49%), Query Frame = 0


Query: 46  CKGSIAECFG-GEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQA 105
           C GSIAEC    EE EFDS+ISRRILA  +YISYGA+RRN+VPCSRRGASYYNCQ GAQA
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQA 104

Query: 106 NPYSRGCSAITRCR 119
           NPYSRGCS ITRCR
Sbjct: 105 NPYSRGCSTITRCR 118

[Close]

BLAST of CmaCh05G011240 vs. TAIR 10
Match: AT2G33775.1 (ralf-like 19 )

HSP 1 Score: 80.5 bits (197), Expect = 1.0e-15
Identity = 39/64 (60.94%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 55  GGEEFEFDSEISRRILATSQ-YISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSA 114
           G  ++  DSE +RR LA  + YISYGALR+N VPCSRRG SYY+C+   +ANPY RGCS 
Sbjct: 42  GELDYLMDSETNRRQLAARRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCSV 101

Query: 115 ITRC 118
           IT C
Sbjct: 102 ITHC 105

The following BLAST results are available for this feature:

BLAST of CmaCh05G011240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9P8	1.4e-30	59.50	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]
Q9SRY3	3.2e-30	63.16	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]
Q945T0	4.1e-30	62.28	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
Q9LUS7	5.0e-28	54.03	Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV...	[more]
Q9MA62	6.6e-28	81.08	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]

BLAST of CmaCh05G011240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15800.1	1.0e-31	59.50	ralf-like 33	[more]
AT1G02900.1	2.2e-31	63.16	rapid alkalinization factor 1	[more]
AT3G16570.1	3.6e-29	54.03	rapid alkalinization factor 23	[more]
AT3G05490.1	4.7e-29	81.08	ralf-like 22	[more]
AT2G33775.1	1.0e-15	60.94	ralf-like 19	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 57..118 e-value: 8.7E-31 score: 106.7
None	No IPR available	PANTHER	PTHR33136:SF50	PROTEIN RALF-LIKE 33	coord: 4..119
None	No IPR available	PANTHER	PTHR33136	RAPID ALKALINIZATION FACTOR-LIKE	coord: 4..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh05G011240.1	CmaCh05G011240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
cellular_component	GO:0005576	extracellular region