CmaCh05G008550 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G008550
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioncytochrome P450 704B1
LocationCma_Chr05: 5843900 .. 5847099 (-)
RNA-Seq ExpressionCmaCh05G008550
SyntenyCmaCh05G008550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGATCAAGAATGGTACAGTTCAAAAAGTGGAGTGTTGGGGGTGGCAACCTGCTTGGTTTGGACATGGATGATTATCCATAGATGGAACCAAAGGAAGAACACAGGTCCCAAGACATGGCCACTCCTAGGAGCCGCATTGGAGCAGCTCCTGAACTACCATAGAATGCACGACTGGCTTCTTGGTTACCTTAATCTCTCCAAAACTGTTGTGGTTCCTATGCCTTTCACTACTTATACTTACATTGCCGACCCTGCCAATGTCCAGCACATTCTCAAGACCAACTTCCCCAACTACCCTAAGGGCGACGCCTACCATTCCTACATGGAAGTGCTTCTTGGAGATGGGATCTTCAATGTGGATGGAGAGCTTTGGAGGAAGCAAAGGAAGACTGCTAGCTTCGAGTTTGCTTCAAAGAATTTGAGGGATTTCAGTGCCTTGGTTTTTAGAGAGTATAGCCTCAAGCTCTCGGATATTTTGACACGGGCGTCTTTGCATAACCAGCAGGTTGACATACAGGTGATTTCTGTTTGGAATCATGACCTTTTACAATTGTATGATATTGTCCACTTTGAGTATAAGTTCTCAGGGTTTCATACTAATGGAGATGTATTATTTACTTATAAACCCATGATCATTTCGTAAGTCAGCTAATGTAGGACTCCCTCCCAACAATCTTCTCCTCGAACGAAATACACCATAGAGTCTCCCTTGACGTCTATGTAGCCCTTGATCGAGGCTCGACTCTTTTCTCTAGAGCTCTCGAACAAAGTACACTTTTTATTCGACACTTGAGTCACTTTTGACTACACTTTTGAAGGTACACCATAGAGCCTCCCCTGAGGCCTATGTAGCCCTCGAACAACCTCCCCTTAATCAAAGCTCGACTCGTTTCTTTAGAGCCCTTGAACAAAGTACACCCTTTGTTTGACACTTGAGTCACTTTTGACTATACTTTTGACTACACTTTCGAGGGTCCCAACTTATTTGTTTGACATATGAAGATTCTATTAACATGACTAAGTTAAGGGCATAACTTTGATACCATGTTAAAAATATCGACTCTCCATAATGATATGATATTGTCCACTTTGAGCATAAGCTCTAGTGGTTTTGCTTTGGACTTCCTCAAAAAGTCTCGTACCAATGGAGATGTATTCTTTACTTATAAACTCAAGATCATTCTCTAAATTAACCCAAACCTCTCAACCATCCTCCATCAGTTCTGACAAGCCATGGTTGAAATTATTGGTGCAGGAATTGCTGATGAGAATGACTTTGGACTCCATATGCAAGGTGGGATTCGGGGTTGAAATTGGATCATTGGCTCCAAATTTGCCAGAAAATGGGTTCGCTAAGGCCTTTGACACAGCAAACATGATTGTCACCCTTCGCTTCATCGATCCTTTCTGGAAGATTAAAAGATTTCTGAATATGGGGTCCGAAGCTCTTCTTCAGAAGTCCATCAAAGTCATCGACCACTTCACCTACTCTGTCATTGCCACAAGGAAAAAAGAGATCCAACAAGCTCCAACCACCCCCAATAAGGTCATTTTATCGTTACCCTTTTACACTAACTTCATTCACTCACTTACTTATGCTATGTTATGACATGACAGATAAAACATGACATATTGTCGAGATTTATCGAGTTAGGGGAAGACCCAAATAACAATTTCACGAACAAAAGCTTAAGAGATGTCGTTCTAAACTTCGTGATTGCTGGACGTGACACAACAGCTGCAACTCTTTCATGGGCTATTTATATGGTAATGACTCATAGCCATGTAGCTGACAGAGTTTACTCAGAGCTCAAGAGCTTTGAAGAAGAAAGAGCCAAAGAAGAGGGAATTTCATTGCTTCACCATCATCCATCTGATATCCAATCCTTTGAGAGAAGAGTAACGCAGTTTGCAGAACTGCTTAATTATGATTCTCTTAATAGATTACATTATTTGCATGCTATGATAACTGAGACGCTACGCCTATACCCTGCAGTCCCTCAGGTATAAAGTATTAGTACTTATAAACCATTAAAGCTTTGGTTTTTGAACAATGAAATATTTTATGTTATAAGTATTTAGGATCCGAAGGGGGTTTTGGAGGATGATGTATTGCCTGATGGGACGAAGGTAAGAGCTGGAGGGATGGTGACATATGTTCCTTACTCAATGGGAAGAATGGAATACAATTGGGGTTCTGATGCAACTTCTTTTAACCCTCAAAGATGGCTCAAACAAGACGTTTTTCATAACGAATCTCCGTTCAAATTCACAGCATTTCAGGTCATTATTCCTTTATTCTGTTATACATATGTTGAGAGAGGAGTCTTATATTAGCTAATTAAGGGGTTGATTATGAGTTTATAAGTAAGGAATACATCTCCATTGATAATGTCATACCATTTGTGGAGGTATGTGATTCCTTACATGATATCAGAGTCATGCCCTTAACTTAGCTATGTCAATAGAATCCTCGAATGTCCAACAAAGAAGTTGCGAGTCTCAAAGTTATAGTCAAAAGTGACTCAAGTGTCGAACAAAGGGTGTAATTTGTTCCTGGGCTCCAAAGAAGTCGAGCCTCAATTAAGTACGTTGTTAGTATCAGAGTCATATTCTTAACTGTCATGTCAATAGAATCCTCAATTGTCGAACAAGGAAGTTTTGAGCCTCGAAGGTGTAGTCAAAAGTGACTCAAGTGTCGAACAAAGGGTTTACTTTGTTTGAGGGCTCCAAAGGAGTCGAGCCTTAATTAAGAAGAGGTTATTCGAGGGGTTCATAGGTCTCAAGGGAGGCTCTATGGTGTACTTTATTTGAGGGGAGAATTTGGTGAGAGTGGAGTTTCATATTGGCTAATTAAGGGGTTGATCACGAGTTCATAAGCAAGAAATACATCTCTATTGGTACGAGACATTTTGGGGAAGCCCAAAACAAAGCCATTAGAGCTTACGCTCAAAGCGGACAATATCATACCATCGTAAAGAGCCGTGATTCCTAAGAATATATTATTTAATGCAGGCAGGACCAAGGATCTGCATGGGGAAAGACTCGGCATATCTACAGATGAAAATGGCGCTTGCCATTTTGTGCAGATTCTACAAATTTAGTTTGGTGAGTGGGCATGAAGTGAGCTACAGAATGATGACTATTCTGTCCATGGCCAATGGCTTGAAAGTTACCGTAGCTCGACGCTCTTAA

mRNA sequence

ATGGTTGATCAAGAATGGTACAGTTCAAAAAGTGGAGTGTTGGGGGTGGCAACCTGCTTGGTTTGGACATGGATGATTATCCATAGATGGAACCAAAGGAAGAACACAGGTCCCAAGACATGGCCACTCCTAGGAGCCGCATTGGAGCAGCTCCTGAACTACCATAGAATGCACGACTGGCTTCTTGGTTACCTTAATCTCTCCAAAACTGTTGTGGTTCCTATGCCTTTCACTACTTATACTTACATTGCCGACCCTGCCAATGTCCAGCACATTCTCAAGACCAACTTCCCCAACTACCCTAAGGGCGACGCCTACCATTCCTACATGGAAGTGCTTCTTGGAGATGGGATCTTCAATGTGGATGGAGAGCTTTGGAGGAAGCAAAGGAAGACTGCTAGCTTCGAGTTTGCTTCAAAGAATTTGAGGGATTTCAGTGCCTTGGTTTTTAGAGAGTATAGCCTCAAGCTCTCGGATATTTTGACACGGGCGTCTTTGCATAACCAGCAGGTTGACATACAGGAATTGCTGATGAGAATGACTTTGGACTCCATATGCAAGGTGGGATTCGGGGTTGAAATTGGATCATTGGCTCCAAATTTGCCAGAAAATGGGTTCGCTAAGGCCTTTGACACAGCAAACATGATTGTCACCCTTCGCTTCATCGATCCTTTCTGGAAGATTAAAAGATTTCTGAATATGGGGTCCGAAGCTCTTCTTCAGAAGTCCATCAAAGTCATCGACCACTTCACCTACTCTGTCATTGCCACAAGGAAAAAAGAGATCCAACAAGCTCCAACCACCCCCAATAAGATAAAACATGACATATTGTCGAGATTTATCGAGTTAGGGGAAGACCCAAATAACAATTTCACGAACAAAAGCTTAAGAGATGTCGTTCTAAACTTCGTGATTGCTGGACGTGACACAACAGCTGCAACTCTTTCATGGGCTATTTATATGGTAATGACTCATAGCCATGTAGCTGACAGAGTTTACTCAGAGCTCAAGAGCTTTGAAGAAGAAAGAGCCAAAGAAGAGGGAATTTCATTGCTTCACCATCATCCATCTGATATCCAATCCTTTGAGAGAAGAGTAACGCAGTTTGCAGAACTGCTTAATTATGATTCTCTTAATAGATTACATTATTTGCATGCTATGATAACTGAGACGCTACGCCTATACCCTGCAGTCCCTCAGGATCCGAAGGGGGTTTTGGAGGATGATGTATTGCCTGATGGGACGAAGGTAAGAGCTGGAGGGATGGTGACATATGTTCCTTACTCAATGGGAAGAATGGAATACAATTGGGGTTCTGATGCAACTTCTTTTAACCCTCAAAGATGGCTCAAACAAGACGTTTTTCATAACGAATCTCCGTTCAAATTCACAGCATTTCAGGCAGGACCAAGGATCTGCATGGGGAAAGACTCGGCATATCTACAGATGAAAATGGCGCTTGCCATTTTGTGCAGATTCTACAAATTTAGTTTGGTGAGTGGGCATGAAGTGAGCTACAGAATGATGACTATTCTGTCCATGGCCAATGGCTTGAAAGTTACCGTAGCTCGACGCTCTTAA

Coding sequence (CDS)

ATGGTTGATCAAGAATGGTACAGTTCAAAAAGTGGAGTGTTGGGGGTGGCAACCTGCTTGGTTTGGACATGGATGATTATCCATAGATGGAACCAAAGGAAGAACACAGGTCCCAAGACATGGCCACTCCTAGGAGCCGCATTGGAGCAGCTCCTGAACTACCATAGAATGCACGACTGGCTTCTTGGTTACCTTAATCTCTCCAAAACTGTTGTGGTTCCTATGCCTTTCACTACTTATACTTACATTGCCGACCCTGCCAATGTCCAGCACATTCTCAAGACCAACTTCCCCAACTACCCTAAGGGCGACGCCTACCATTCCTACATGGAAGTGCTTCTTGGAGATGGGATCTTCAATGTGGATGGAGAGCTTTGGAGGAAGCAAAGGAAGACTGCTAGCTTCGAGTTTGCTTCAAAGAATTTGAGGGATTTCAGTGCCTTGGTTTTTAGAGAGTATAGCCTCAAGCTCTCGGATATTTTGACACGGGCGTCTTTGCATAACCAGCAGGTTGACATACAGGAATTGCTGATGAGAATGACTTTGGACTCCATATGCAAGGTGGGATTCGGGGTTGAAATTGGATCATTGGCTCCAAATTTGCCAGAAAATGGGTTCGCTAAGGCCTTTGACACAGCAAACATGATTGTCACCCTTCGCTTCATCGATCCTTTCTGGAAGATTAAAAGATTTCTGAATATGGGGTCCGAAGCTCTTCTTCAGAAGTCCATCAAAGTCATCGACCACTTCACCTACTCTGTCATTGCCACAAGGAAAAAAGAGATCCAACAAGCTCCAACCACCCCCAATAAGATAAAACATGACATATTGTCGAGATTTATCGAGTTAGGGGAAGACCCAAATAACAATTTCACGAACAAAAGCTTAAGAGATGTCGTTCTAAACTTCGTGATTGCTGGACGTGACACAACAGCTGCAACTCTTTCATGGGCTATTTATATGGTAATGACTCATAGCCATGTAGCTGACAGAGTTTACTCAGAGCTCAAGAGCTTTGAAGAAGAAAGAGCCAAAGAAGAGGGAATTTCATTGCTTCACCATCATCCATCTGATATCCAATCCTTTGAGAGAAGAGTAACGCAGTTTGCAGAACTGCTTAATTATGATTCTCTTAATAGATTACATTATTTGCATGCTATGATAACTGAGACGCTACGCCTATACCCTGCAGTCCCTCAGGATCCGAAGGGGGTTTTGGAGGATGATGTATTGCCTGATGGGACGAAGGTAAGAGCTGGAGGGATGGTGACATATGTTCCTTACTCAATGGGAAGAATGGAATACAATTGGGGTTCTGATGCAACTTCTTTTAACCCTCAAAGATGGCTCAAACAAGACGTTTTTCATAACGAATCTCCGTTCAAATTCACAGCATTTCAGGCAGGACCAAGGATCTGCATGGGGAAAGACTCGGCATATCTACAGATGAAAATGGCGCTTGCCATTTTGTGCAGATTCTACAAATTTAGTTTGGTGAGTGGGCATGAAGTGAGCTACAGAATGATGACTATTCTGTCCATGGCCAATGGCTTGAAAGTTACCGTAGCTCGACGCTCTTAA

Protein sequence

MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALLQKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVVLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS
Homology
BLAST of CmaCh05G008550 vs. ExPASy Swiss-Prot
Match: Q9C788 (Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 1.9e-228
Identity = 383/519 (73.80%), Postives = 441/519 (84.97%), Query Frame = 0

Query: 16  VATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNLSKTVVVPM 75
           V  C+V +W+ +HRW QR  +GPKTWPL+GAA+EQL N+ RMHDWL+ YL  S+TVVVPM
Sbjct: 6   VIACMVTSWIFLHRWGQRNKSGPKTWPLVGAAIEQLTNFDRMHDWLVEYLYNSRTVVVPM 65

Query: 76  PFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVDGELWRKQRKTASF 135
           PFTTYTYIADP NV+++LKTNF NYPKG+ YHSYMEVLLGDGIFN DGELWRKQRKTASF
Sbjct: 66  PFTTYTYIADPINVEYVLKTNFSNYPKGETYHSYMEVLLGDGIFNSDGELWRKQRKTASF 125

Query: 136 EFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRMTLDSICKVGFGVEIG 195
           EFASKNLRDFS +VF+EYSLKL  IL++AS   QQVD+QELLMRMTLDSICKVGFGVEIG
Sbjct: 126 EFASKNLRDFSTVVFKEYSLKLFTILSQASFKEQQVDMQELLMRMTLDSICKVGFGVEIG 185

Query: 196 SLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALLQKSIKVIDHFTYSVI 255
           +LAP LPEN FAKAFDTAN+IVTLRFIDP WK+K+FLN+GSEALL KSIKV++ FTYSVI
Sbjct: 186 TLAPELPENHFAKAFDTANIIVTLRFIDPLWKMKKFLNIGSEALLGKSIKVVNDFTYSVI 245

Query: 256 ATRKKEIQQAPTTP--------NKIKHDILSRFIELGEDPNNNFTNKSLRDVVLNFVIAG 315
             RK E+ +A  +P        NK+KHDILSRFIE+ +DP++  T KSLRD+VLNFVIAG
Sbjct: 246 RRRKAELLEAQISPTNNNNNNNNKVKHDILSRFIEISDDPDSKETEKSLRDIVLNFVIAG 305

Query: 316 RDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQSFERRVT 375
           RDTTA TL+WAIYM+M + +VA+++YSEL+  E+E A+    SL  +   D  SF  +VT
Sbjct: 306 RDTTATTLTWAIYMIMMNENVAEKLYSELQELEKESAEATNTSLHQYDTEDFNSFNEKVT 365

Query: 376 QFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAGGMVTYVP 435
           +FA LLNYDSL +LHYLHA+ITETLRLYPAVPQDPKGVLEDD+LP+GTKV+AGGMVTYVP
Sbjct: 366 EFAGLLNYDSLGKLHYLHAVITETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMVTYVP 425

Query: 436 YSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAYLQMKMAL 495
           YSMGRMEYNWGSDA  F P+RWLK  VF N SPFKFTAFQAGPRIC+GKDSAYLQMKMA+
Sbjct: 426 YSMGRMEYNWGSDAALFKPERWLKDGVFQNASPFKFTAFQAGPRICLGKDSAYLQMKMAM 485

Query: 496 AILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 527
           AILCRFYKF LV  H V YRMMTILSMA+GLKVTV+RRS
Sbjct: 486 AILCRFYKFHLVPNHPVKYRMMTILSMAHGLKVTVSRRS 524

BLAST of CmaCh05G008550 vs. ExPASy Swiss-Prot
Match: Q50EK3 (Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.0e-125
Identity = 242/514 (47.08%), Postives = 327/514 (63.62%), Query Frame = 0

Query: 19  CLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNLSKTVVVPMPFT 78
           C +W    +  W ++K   P   P++G  L   +N+ R+HD+        KT  V  P  
Sbjct: 19  CSLWIASYLRNW-RKKGVYP---PVVGTMLNHAINFERLHDYHTDQAQRYKTFRVVYPTC 78

Query: 79  TYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFA 138
           +Y +  DP NV+HILKTNF NY KG   +  M+ LLGDGIFNVDG+ WR+QRK AS EFA
Sbjct: 79  SYVFTTDPVNVEHILKTNFANYDKGTFNYDIMKDLLGDGIFNVDGDKWRQQRKLASSEFA 138

Query: 139 SKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRMTLDSICKVGFGVEIGSL- 198
           SK L+DFS+ VF   + KL++IL +A+  N  V++Q+L MR +LDSICKV FG++I SL 
Sbjct: 139 SKVLKDFSSGVFCNNAAKLANILAQAAKLNLSVEMQDLFMRSSLDSICKVVFGIDINSLS 198

Query: 199 ---APNLPENGFAKAFDTANMIVTLR-FIDPFWKIKRFLNMGSEALLQKSIKVIDHFTYS 258
              A + PE  FAKAFD AN +V  R  +  FWK++RF N+GSEA+L+ +IK++D F Y 
Sbjct: 199 SSKAESGPEASFAKAFDVANAMVFHRHMVGSFWKVQRFFNVGSEAILRDNIKMVDDFLYK 258

Query: 259 VIATRKKEIQQAPTTPNKIKHDILSRFIELGE-DPNNNFTNKSLRDVVLNFVIAGRDTTA 318
           VI  R++E+  A      ++ DILSR+I + + + +   ++K LRDV+LNF++A RDTTA
Sbjct: 259 VIHFRRQEMFSA--EKENVRPDILSRYIIISDKETDGKVSDKYLRDVILNFMVAARDTTA 318

Query: 319 ATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQSFERRVTQFAEL 378
             LSW IYM+  H HV            +E+  EE IS    H     +    +  FA+ 
Sbjct: 319 IALSWFIYMLCKHQHV------------QEKLLEEIISSTSVHEDQYSTECNDIASFAQS 378

Query: 379 LNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAGGMVTYVPYSMGR 438
           L  ++L ++HYLHA ++ETLRLYPA+P D K V+ +D LPDG KV+ G  V ++PY+MGR
Sbjct: 379 LTDEALGKMHYLHASLSETLRLYPALPVDGKYVVNEDTLPDGFKVKKGDSVNFLPYAMGR 438

Query: 439 MEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAYLQMKMALAILCR 498
           M Y WG DA  F P+RW++  +FH +SPFKF AFQAGPR C+GKD AYLQMK+  A+L R
Sbjct: 439 MSYLWGDDAKEFKPERWIQDGIFHPKSPFKFPAFQAGPRTCLGKDFAYLQMKIVAAVLVR 498

Query: 499 FYKFSLVSGHEVSYRMMTILSM-ANGLKVTVARR 526
           F+KF  V   EV YR M  L M  +GL V V  R
Sbjct: 499 FFKFEAVKTKEVRYRTMLTLHMNEDGLNVQVTPR 514

BLAST of CmaCh05G008550 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 4.7e-102
Identity = 210/532 (39.47%), Postives = 300/532 (56.39%), Query Frame = 0

Query: 9   SKSGVLGVATCLVWTW-MIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNL 68
           S + +L       W W   I RW +    GP+ WP+LG+    +    RMHDW+   L  
Sbjct: 4   SNTMLLVAVVAAYWLWFQRISRWLK----GPRVWPVLGSLPGLIEQRDRMHDWITENLRA 63

Query: 69  S----KTVVVPMPFTT-----YTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGI 128
                +T +  +PF        T   DP N++H+LKT F NYPKG  + +     LG GI
Sbjct: 64  CGGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGI 123

Query: 129 FNVDGELWRKQRKTASFEFASKNLRD-FSALVFREYSLKLSDILTRASLHNQQVDIQELL 188
           FN DG+ W  QRKTA+ EF ++ LR      V R   L+   IL  A  + + VD+Q+L+
Sbjct: 124 FNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLI 183

Query: 189 MRMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDP--FWKIKRFLNMG 248
           +R+T D+IC + FG +  + AP LPENGFA AFD A      RFI P   W++K++L +G
Sbjct: 184 LRLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLG 243

Query: 249 SEALLQKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKS 308
            E  L +S+  ID +  +VI TRK+E+     +  +   D+LSRF+   +  + +++   
Sbjct: 244 LEVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFM---KKKDQSYSETF 303

Query: 309 LRDVVLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHH 368
           LR V LNF++AGRDT++  LSW  +++ TH  V D++  E+ S            L+   
Sbjct: 304 LRHVALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSV-----------LIETR 363

Query: 369 PSDIQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGT 428
            +D+ S+       AE L +D ++RL YL A ++ETLRLYP+VP+D K V+ DD+LPDGT
Sbjct: 364 GTDVSSWT------AEPLEFDEVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGT 423

Query: 429 KVRAGGMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQD--VFHNESPFKFTAFQAGPRIC 488
            V AG  VTY  Y+ GRM+  WG D   F P+RW+  D   F N   ++F AF AGPRIC
Sbjct: 424 FVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRIC 483

Query: 489 MGKDSAYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARR 526
           +GKD AYLQMK   A +   ++ ++  GH+V  +M   L M NGL V V +R
Sbjct: 484 LGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFMKNGLLVNVHKR 511

BLAST of CmaCh05G008550 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.0e-100
Identity = 209/520 (40.19%), Postives = 292/520 (56.15%), Query Frame = 0

Query: 22  WTWM-IIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNLS----KTVVVPMP 81
           W W   I RW +    GP+ WPLLG+    +    RMH+W+   L       +T +  +P
Sbjct: 17  WLWFKRISRWLK----GPRVWPLLGSLPGLIEQRDRMHEWITENLRACGGTYQTCIFAVP 76

Query: 82  FTT-----YTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVDGELWRKQRK 141
           F        T   DP N++H+LKT F NYPKG  + S    LLG GIFN DG+ W  QRK
Sbjct: 77  FLAKKQGLVTVTCDPKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDTWLFQRK 136

Query: 142 TASFEFASKNLRD-FSALVFREYSLKLSDILTRASLHNQQVDIQELLMRMTLDSICKVGF 201
           TA+ EF ++ LR      V R   L+   IL  A  + + VD+Q+L++R+T D+IC + F
Sbjct: 137 TAALEFTTRTLRQAMGRWVNRGIKLRFCPILATAQDNAEPVDLQDLILRLTFDNICGLAF 196

Query: 202 GVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDP--FWKIKRFLNMGSEALLQKSIKVID 261
           G +  + AP LPENGFA AFD A      RFI P   WK+K++L +G E  L +S+  ID
Sbjct: 197 GKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGLEVSLSRSLGEID 256

Query: 262 HFTYSVIATRKKEIQQAPTTPNKIKH-DILSRFIELGEDPNNNFTNKSLRDVVLNFVIAG 321
            +  +VI TRK+E+     +    +H D+LSRF+        ++++  L+ V LNF++AG
Sbjct: 257 EYLAAVINTRKQELMSQQESGTHQRHDDLLSRFM---MKKTESYSDTFLQHVALNFILAG 316

Query: 322 RDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQSFERRVT 381
           RDT++  LSW  +++  H  V D++  E+ S   E    + ++     P           
Sbjct: 317 RDTSSVALSWFFWLITMHPTVEDKIVREICSVLIETRGTDDVASWTEEP----------- 376

Query: 382 QFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAGGMVTYVP 441
                L +D ++RL YL A I+ETLRLYP+VP+D K V  DDVLPDGT V AG  VTY  
Sbjct: 377 -----LGFDEIDRLVYLKAAISETLRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSI 436

Query: 442 YSMGRMEYNWGSDATSFNPQRWLK--QDVFHNESPFKFTAFQAGPRICMGKDSAYLQMKM 501
           Y+ GRM+  WG D   FNP+RW+      F N   ++F AF AGPRIC+GKD AYLQMK 
Sbjct: 437 YAAGRMKSTWGEDCLEFNPERWISPIDGKFINHDQYRFVAFNAGPRICLGKDLAYLQMKT 496

Query: 502 ALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARR 526
             A +   ++ ++V GH+V  +M   L M NGL V + +R
Sbjct: 497 IAAAVLLRHRLTVVPGHKVEQKMSLTLFMKNGLLVNLYKR 513

BLAST of CmaCh05G008550 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 7.5e-100
Identity = 205/519 (39.50%), Postives = 310/519 (59.73%), Query Frame = 0

Query: 20  LVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNLS----KTVVVPM 79
           L+ T++I     +R   GP+ WPL+G+    + N HRMHD++   L +     +T + P+
Sbjct: 12  LIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMCGGTYQTCIFPI 71

Query: 80  PFTT-----YTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVDGELWRKQR 139
           PF        T   DP N++HILKT F NYPKG ++ S    LLGDGIFN DG+ WR QR
Sbjct: 72  PFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIFNSDGDTWRFQR 131

Query: 140 KTASFEFASKNLRDFSA-LVFREYSLKLSDILTRASLHNQQVDIQELLMRMTLDSICKVG 199
           KTA+ EF ++ LR   A  V R    +L  IL  A    + +D+Q++L+R+T D+IC + 
Sbjct: 132 KTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLLRLTFDNICGLT 191

Query: 200 FGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDP--FWKIKRFLNMGSEALLQKSIKVI 259
           FG +  +L+P  PENGFA AFD A      RFI P   WKI+++L +G E  + +SI  +
Sbjct: 192 FGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGLEDDMSRSISHV 251

Query: 260 DHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVVLNFVIAG 319
           D++   +I TRK E+       ++   D+LSRF++  E    ++++K L+ V LNF++AG
Sbjct: 252 DNYLSEIINTRKLELLGQQQDESR-HDDLLSRFMKKKE----SYSDKYLKYVALNFILAG 311

Query: 320 RDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQSFERRVT 379
           RDT++  +SW  ++V  +  V +++ +E+             ++L      I++ +  V+
Sbjct: 312 RDTSSVAMSWFFWLVSLNPRVEEKIINEI------------CTIL------IKTRDTNVS 371

Query: 380 QFA-ELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAGGMVTYV 439
           ++  E L +D +++L YL A ++ETLRLYP+VP+D K V+ +DVLPDGT V +G  VTY 
Sbjct: 372 KWTDEPLTFDEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYS 431

Query: 440 PYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAYLQMKMA 499
            YS+GRM++ WG D   F P+RWL++      + +KF AF AGPRIC+GKD AYLQMK  
Sbjct: 432 IYSVGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSI 491

Query: 500 LAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARR 526
            A +   ++ ++  GH V  +M   L M  GLK+ V +R
Sbjct: 492 TASILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKR 507

BLAST of CmaCh05G008550 vs. ExPASy TrEMBL
Match: A0A6J1ICQ4 (cytochrome P450 704B1 OS=Cucurbita maxima OX=3661 GN=LOC111471352 PE=3 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.0e-310
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60
           MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW
Sbjct: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60

Query: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120
           LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN
Sbjct: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120

Query: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM 180
           VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM
Sbjct: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM 180

Query: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL 240
           TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL
Sbjct: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL 240

Query: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300
           QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV
Sbjct: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300

Query: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360
           LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ
Sbjct: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360

Query: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420
           SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG
Sbjct: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420

Query: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY 480
           GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY
Sbjct: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY 480

Query: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 527
           LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS
Sbjct: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 526

BLAST of CmaCh05G008550 vs. ExPASy TrEMBL
Match: A0A6J1G378 (cytochrome P450 704B1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450376 PE=3 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 1.9e-308
Identity = 522/526 (99.24%), Postives = 524/526 (99.62%), Query Frame = 0

Query: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60
           MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW
Sbjct: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60

Query: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120
           LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN
Sbjct: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120

Query: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM 180
           VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVD+QELLMRM
Sbjct: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDVQELLMRM 180

Query: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL 240
           TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLN+GSEALL
Sbjct: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNLGSEALL 240

Query: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300
           QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV
Sbjct: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300

Query: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360
           LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEE ISLLHHHPSDIQ
Sbjct: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEERISLLHHHPSDIQ 360

Query: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420
           SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG
Sbjct: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420

Query: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY 480
           GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQ VFHNESPFKFTAFQAGPRICMGKDSAY
Sbjct: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQGVFHNESPFKFTAFQAGPRICMGKDSAY 480

Query: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 527
           LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS
Sbjct: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 526

BLAST of CmaCh05G008550 vs. ExPASy TrEMBL
Match: A0A6J1DQX4 (cytochrome P450 704B1 OS=Momordica charantia OX=3673 GN=LOC111023497 PE=3 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 7.2e-271
Identity = 462/527 (87.67%), Postives = 488/527 (92.60%), Query Frame = 0

Query: 3   DQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLL 62
           ++ W S K+ V+G A CLVW+W+  HRW QRK  GPKTWPL+GAA+EQLLNYHRMHDWLL
Sbjct: 9   EEGWCSVKTLVVGAAACLVWSWIAAHRWKQRKKKGPKTWPLVGAAIEQLLNYHRMHDWLL 68

Query: 63  GYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVD 122
           GYL LSKTVVVPMPFTTYTYIA P NV HILKTNF NYPKGDAYHSYMEVLLGDGIFNVD
Sbjct: 69  GYLKLSKTVVVPMPFTTYTYIAHPDNVHHILKTNFANYPKGDAYHSYMEVLLGDGIFNVD 128

Query: 123 GELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRMTL 182
           GELWRKQRKTASFEFASKNLRDFSALVFREYSLKLS ILT ASLHNQQVD+QELLMRMTL
Sbjct: 129 GELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSSILTSASLHNQQVDMQELLMRMTL 188

Query: 183 DSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALLQK 242
           DSICKVGFGVEIGSLAPNLPEN FA+AFDTAN+IVTLRFIDP WKIKRFLN+GSEALL K
Sbjct: 189 DSICKVGFGVEIGSLAPNLPENCFAQAFDTANIIVTLRFIDPLWKIKRFLNLGSEALLDK 248

Query: 243 SIKVIDHFTYSVIATRKKEIQQAPTTPN---KIKHDILSRFIELGEDPNNNFTNKSLRDV 302
           SIK+ID FTYSVIATRKKEI++A TT N   KIKHDILSRFIELG+DPNNNFTNKSLRDV
Sbjct: 249 SIKIIDDFTYSVIATRKKEIEEARTTGNDGKKIKHDILSRFIELGDDPNNNFTNKSLRDV 308

Query: 303 VLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLH-HHPSD 362
           VLNFVIAGRDTTAATLSWAIYMVMTHSHVAD++YSEL SFEE RAKEEGISLL+ HH +D
Sbjct: 309 VLNFVIAGRDTTAATLSWAIYMVMTHSHVADKLYSELTSFEEARAKEEGISLLNQHHSTD 368

Query: 363 IQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVR 422
           ++SFERRV QFAELLNYDSL RLHYLHAMITETLRLYPAVPQDPKG+LEDDVLPDGTKVR
Sbjct: 369 LESFERRVKQFAELLNYDSLARLHYLHAMITETLRLYPAVPQDPKGILEDDVLPDGTKVR 428

Query: 423 AGGMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDS 482
           AGGMVTYVPYSMGRMEYNWG DA SFNP+RWLKQ +FHNESPFKFTAFQAGPRICMGKDS
Sbjct: 429 AGGMVTYVPYSMGRMEYNWGCDAASFNPERWLKQGIFHNESPFKFTAFQAGPRICMGKDS 488

Query: 483 AYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARR 526
           AYLQMKMALAILCRFYKF LVSGH+V YRMMTILSMANGLKVTV RR
Sbjct: 489 AYLQMKMALAILCRFYKFKLVSGHQVKYRMMTILSMANGLKVTVDRR 535

BLAST of CmaCh05G008550 vs. ExPASy TrEMBL
Match: A0A1S3B4S2 (cytochrome P450 704B1 OS=Cucumis melo OX=3656 GN=LOC103485770 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.7e-262
Identity = 451/539 (83.67%), Postives = 485/539 (89.98%), Query Frame = 0

Query: 3   DQEWYSSKSG---------VLGVATCLVWTWMIIHRWNQRK--NTGPKTWPLLGAALEQL 62
           ++EW+   S          VLGV T +    +I+HRWN+RK    GPKTWP++GAA+EQ 
Sbjct: 18  EEEWHMRSSNLRRSVVVVLVLGVITIVTCWIIIVHRWNERKKMRRGPKTWPIVGAAIEQF 77

Query: 63  LNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYME 122
           +NYHRMHDW+LGYLNL KTVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSYME
Sbjct: 78  VNYHRMHDWILGYLNLYKTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYME 137

Query: 123 VLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQV 182
           VLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+YSLKLS ILTRASL NQQ+
Sbjct: 138 VLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYSLKLSTILTRASLLNQQL 197

Query: 183 DIQELLMRMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRF 242
           D+QELL RMTLDSICKVGFGVEIGSLAPNLPEN FAKAFDTANMIVTLRFIDP WKIKRF
Sbjct: 198 DMQELLTRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIKRF 257

Query: 243 LNMGSEALLQKSIKVIDHFTYSVIATRKKEIQQAPTTP----NKIKHDILSRFIELGEDP 302
           LN+GSEALL KSIK+ID FTYSVIATRKKEIQ+A TTP    NKIKHDILSRFIELGEDP
Sbjct: 258 LNLGSEALLDKSIKIIDDFTYSVIATRKKEIQEAQTTPTEPNNKIKHDILSRFIELGEDP 317

Query: 303 NNNFTNKSLRDVVLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEE 362
           NN FT+KSLRDVVLNFVIAGRDTTAATLSW+IYM+MTHS V+ ++YSELKSFEEERAKE 
Sbjct: 318 NNKFTDKSLRDVVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKEH 377

Query: 363 GISLLHHHPSDIQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLE 422
            ISLL + P+DIQSFERRV QF++LLNYDSL RLHYLHA+ITETLRLYPAVPQDPKG+LE
Sbjct: 378 SISLLQYQPNDIQSFERRVIQFSQLLNYDSLARLHYLHAIITETLRLYPAVPQDPKGILE 437

Query: 423 DDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQ 482
           DD+LPDGTKVRAGGMVTYVPYSMGRMEYNWG DATSFNP+RWLKQ +FHNESPFKFTAFQ
Sbjct: 438 DDILPDGTKVRAGGMVTYVPYSMGRMEYNWGPDATSFNPERWLKQGIFHNESPFKFTAFQ 497

Query: 483 AGPRICMGKDSAYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 527
           AGPRICMGKDSAYLQMKM LAILCRFYKFSLVSGH V+YRMMTILSMANGLKVTVA RS
Sbjct: 498 AGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVNYRMMTILSMANGLKVTVASRS 556

BLAST of CmaCh05G008550 vs. ExPASy TrEMBL
Match: A0A0A0LN46 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108690 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.4e-258
Identity = 444/540 (82.22%), Postives = 482/540 (89.26%), Query Frame = 0

Query: 3   DQEWYS-----SKSGVLGVATCLV------WTWMIIHRWNQRK--NTGPKTWPLLGAALE 62
           ++EWY       +SGV+ +   ++      W  +I+HRWNQRK    GPKTWP++GAA+E
Sbjct: 18  EEEWYMRSSNLRRSGVVALVLVMIAIISACWI-IIVHRWNQRKKMRRGPKTWPIVGAAIE 77

Query: 63  QLLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSY 122
           Q +NYHRMHDW+LGYLNL +TVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSY
Sbjct: 78  QFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSY 137

Query: 123 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQ 182
           MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+Y LKLS ILTRASL NQ
Sbjct: 138 MEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQ 197

Query: 183 QVDIQELLMRMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIK 242
           Q+D+QELLMRMTLDSICKVGFGVEIGSLAPNLPEN FAKAFDTANMIVTLRFIDP WKIK
Sbjct: 198 QIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIK 257

Query: 243 RFLNMGSEALLQKSIKVIDHFTYSVIATRKKEIQQAPTT---PNKIKHDILSRFIELGED 302
           RFLN+GSEALL KSIK ID FTYSVIATRKKEIQ+A TT   PNK+KHDILSRFIELGED
Sbjct: 258 RFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKHDILSRFIELGED 317

Query: 303 PNNNFTNKSLRDVVLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKE 362
           PNN FT+KSLRD+VLNFVIAGRDTTAATLSW+IYM+MTHS V+ ++YSELKSFEEERAKE
Sbjct: 318 PNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKE 377

Query: 363 EGISLLHHHPSDIQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVL 422
             ISLL + P+DIQSF+ RV QF++LLNYDSL RLHYLHA++TETLRLYPAVPQDPKG+L
Sbjct: 378 HSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGIL 437

Query: 423 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAF 482
           EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWG DATSF P+RWLKQ +F NESPFKFTAF
Sbjct: 438 EDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAF 497

Query: 483 QAGPRICMGKDSAYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 527
           QAGPRICMGKDSAYLQMKM LAILCRFYKFSLVSGH V YRMMTILSMANGLKVTVA RS
Sbjct: 498 QAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPRS 556

BLAST of CmaCh05G008550 vs. NCBI nr
Match: XP_022972834.1 (cytochrome P450 704B1 [Cucurbita maxima])

HSP 1 Score: 1074.7 bits (2778), Expect = 4.0e-310
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60
           MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW
Sbjct: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60

Query: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120
           LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN
Sbjct: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120

Query: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM 180
           VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM
Sbjct: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM 180

Query: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL 240
           TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL
Sbjct: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL 240

Query: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300
           QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV
Sbjct: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300

Query: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360
           LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ
Sbjct: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360

Query: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420
           SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG
Sbjct: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420

Query: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY 480
           GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY
Sbjct: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY 480

Query: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 527
           LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS
Sbjct: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 526

BLAST of CmaCh05G008550 vs. NCBI nr
Match: XP_022946229.1 (cytochrome P450 704B1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1067.4 bits (2759), Expect = 4.0e-308
Identity = 522/526 (99.24%), Postives = 524/526 (99.62%), Query Frame = 0

Query: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60
           MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW
Sbjct: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60

Query: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120
           LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN
Sbjct: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120

Query: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM 180
           VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVD+QELLMRM
Sbjct: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDVQELLMRM 180

Query: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL 240
           TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLN+GSEALL
Sbjct: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNLGSEALL 240

Query: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300
           QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV
Sbjct: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300

Query: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360
           LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEE ISLLHHHPSDIQ
Sbjct: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEERISLLHHHPSDIQ 360

Query: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420
           SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG
Sbjct: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420

Query: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY 480
           GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQ VFHNESPFKFTAFQAGPRICMGKDSAY
Sbjct: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQGVFHNESPFKFTAFQAGPRICMGKDSAY 480

Query: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 527
           LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS
Sbjct: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 526

BLAST of CmaCh05G008550 vs. NCBI nr
Match: XP_023545882.1 (cytochrome P450 704B1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.3e-306
Identity = 519/526 (98.67%), Postives = 523/526 (99.43%), Query Frame = 0

Query: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60
           MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW
Sbjct: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60

Query: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120
           LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN
Sbjct: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120

Query: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM 180
           VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLS ILT+ASLHNQQVD+QELLMRM
Sbjct: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSGILTQASLHNQQVDMQELLMRM 180

Query: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL 240
           TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLN+GSEALL
Sbjct: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNLGSEALL 240

Query: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300
           QKSIKVIDHFTYSVIATRK EIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV
Sbjct: 241 QKSIKVIDHFTYSVIATRKNEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300

Query: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360
           LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ
Sbjct: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360

Query: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420
           SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG
Sbjct: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420

Query: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY 480
           GMVTYVPYSMGRMEYNWGSDATSFNP+RWLKQ VFHNESPFKFTAFQAGPRICMGKDSAY
Sbjct: 421 GMVTYVPYSMGRMEYNWGSDATSFNPERWLKQGVFHNESPFKFTAFQAGPRICMGKDSAY 480

Query: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 527
           LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS
Sbjct: 481 LQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 526

BLAST of CmaCh05G008550 vs. NCBI nr
Match: KAG7029959.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1035.4 bits (2676), Expect = 1.7e-298
Identity = 503/506 (99.41%), Postives = 505/506 (99.80%), Query Frame = 0

Query: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60
           MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW
Sbjct: 15  MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 74

Query: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120
           LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN
Sbjct: 75  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 134

Query: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM 180
           VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVD+QELLMRM
Sbjct: 135 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDVQELLMRM 194

Query: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL 240
           TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLN+GSEALL
Sbjct: 195 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNLGSEALL 254

Query: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300
           QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV
Sbjct: 255 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 314

Query: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360
           LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ
Sbjct: 315 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 374

Query: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420
           SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG
Sbjct: 375 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 434

Query: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY 480
           GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQ VFHNESPFKFTAFQAGPRICMGKDSAY
Sbjct: 435 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQGVFHNESPFKFTAFQAGPRICMGKDSAY 494

Query: 481 LQMKMALAILCRFYKFSLVSGHEVSY 507
           LQMKMALAILCRFYKFSLVSGHEVSY
Sbjct: 495 LQMKMALAILCRFYKFSLVSGHEVSY 520

BLAST of CmaCh05G008550 vs. NCBI nr
Match: KAG6598990.1 (Cytochrome P450 704B1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1021.9 bits (2641), Expect = 1.9e-294
Identity = 498/517 (96.32%), Postives = 508/517 (98.26%), Query Frame = 0

Query: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60
           MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW
Sbjct: 1   MVDQEWYSSKSGVLGVATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDW 60

Query: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120
           LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN
Sbjct: 61  LLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFN 120

Query: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRM 180
           VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVD+QELLMRM
Sbjct: 121 VDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDVQELLMRM 180

Query: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALL 240
           TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLN+GSEALL
Sbjct: 181 TLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNLGSEALL 240

Query: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300
           QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV
Sbjct: 241 QKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVV 300

Query: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360
           LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ
Sbjct: 301 LNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQ 360

Query: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420
           SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG
Sbjct: 361 SFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAG 420

Query: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAY 480
           GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQ VFHNESPFKFTAFQAGPRICMGKDSAY
Sbjct: 421 GMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQGVFHNESPFKFTAFQAGPRICMGKDSAY 480

Query: 481 LQMKMALAILCRFYKFSLVSGHEVS-YRMMTILSMAN 517
           LQMKMALAILCRFYKFSL+   +++ +  ++  SMA+
Sbjct: 481 LQMKMALAILCRFYKFSLLDALKIALFNQLSTKSMAS 517

BLAST of CmaCh05G008550 vs. TAIR 10
Match: AT1G69500.1 (cytochrome P450, family 704, subfamily B, polypeptide 1 )

HSP 1 Score: 793.1 bits (2047), Expect = 1.4e-229
Identity = 383/519 (73.80%), Postives = 441/519 (84.97%), Query Frame = 0

Query: 16  VATCLVWTWMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNLSKTVVVPM 75
           V  C+V +W+ +HRW QR  +GPKTWPL+GAA+EQL N+ RMHDWL+ YL  S+TVVVPM
Sbjct: 6   VIACMVTSWIFLHRWGQRNKSGPKTWPLVGAAIEQLTNFDRMHDWLVEYLYNSRTVVVPM 65

Query: 76  PFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVDGELWRKQRKTASF 135
           PFTTYTYIADP NV+++LKTNF NYPKG+ YHSYMEVLLGDGIFN DGELWRKQRKTASF
Sbjct: 66  PFTTYTYIADPINVEYVLKTNFSNYPKGETYHSYMEVLLGDGIFNSDGELWRKQRKTASF 125

Query: 136 EFASKNLRDFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRMTLDSICKVGFGVEIG 195
           EFASKNLRDFS +VF+EYSLKL  IL++AS   QQVD+QELLMRMTLDSICKVGFGVEIG
Sbjct: 126 EFASKNLRDFSTVVFKEYSLKLFTILSQASFKEQQVDMQELLMRMTLDSICKVGFGVEIG 185

Query: 196 SLAPNLPENGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALLQKSIKVIDHFTYSVI 255
           +LAP LPEN FAKAFDTAN+IVTLRFIDP WK+K+FLN+GSEALL KSIKV++ FTYSVI
Sbjct: 186 TLAPELPENHFAKAFDTANIIVTLRFIDPLWKMKKFLNIGSEALLGKSIKVVNDFTYSVI 245

Query: 256 ATRKKEIQQAPTTP--------NKIKHDILSRFIELGEDPNNNFTNKSLRDVVLNFVIAG 315
             RK E+ +A  +P        NK+KHDILSRFIE+ +DP++  T KSLRD+VLNFVIAG
Sbjct: 246 RRRKAELLEAQISPTNNNNNNNNKVKHDILSRFIEISDDPDSKETEKSLRDIVLNFVIAG 305

Query: 316 RDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQSFERRVT 375
           RDTTA TL+WAIYM+M + +VA+++YSEL+  E+E A+    SL  +   D  SF  +VT
Sbjct: 306 RDTTATTLTWAIYMIMMNENVAEKLYSELQELEKESAEATNTSLHQYDTEDFNSFNEKVT 365

Query: 376 QFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAGGMVTYVP 435
           +FA LLNYDSL +LHYLHA+ITETLRLYPAVPQDPKGVLEDD+LP+GTKV+AGGMVTYVP
Sbjct: 366 EFAGLLNYDSLGKLHYLHAVITETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMVTYVP 425

Query: 436 YSMGRMEYNWGSDATSFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAYLQMKMAL 495
           YSMGRMEYNWGSDA  F P+RWLK  VF N SPFKFTAFQAGPRIC+GKDSAYLQMKMA+
Sbjct: 426 YSMGRMEYNWGSDAALFKPERWLKDGVFQNASPFKFTAFQAGPRICLGKDSAYLQMKMAM 485

Query: 496 AILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARRS 527
           AILCRFYKF LV  H V YRMMTILSMA+GLKVTV+RRS
Sbjct: 486 AILCRFYKFHLVPNHPVKYRMMTILSMAHGLKVTVSRRS 524

BLAST of CmaCh05G008550 vs. TAIR 10
Match: AT2G45510.1 (cytochrome P450, family 704, subfamily A, polypeptide 2 )

HSP 1 Score: 394.0 bits (1011), Expect = 1.8e-109
Identity = 212/505 (41.98%), Postives = 297/505 (58.81%), Query Frame = 0

Query: 24  WMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYI 83
           +++I  +  +     +  P+     + L +   ++D+         T     P  +    
Sbjct: 24  YLMIRIFTGKSRNDKRYAPVHATVFDLLFHSDELYDYETEIAREKPTYRFLSPGQSEILT 83

Query: 84  ADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLR 143
           ADP NV+HILKT F NY KG +    M  LLG GIF VDGE WR+QRK +SFEF+++ LR
Sbjct: 84  ADPRNVEHILKTRFDNYSKGHSSRENMADLLGHGIFAVDGEKWRQQRKLSSFEFSTRVLR 143

Query: 144 DFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRMTLDSICKVGFGVEIGSLAPNLPE 203
           DFS  VFR  + KL   ++  +L  +  D Q+LLMR TLDSI KVGFGVE+  L     E
Sbjct: 144 DFSCSVFRRNASKLVGFVSEFALSGKAFDAQDLLMRCTLDSIFKVGFGVELKCLDGFSKE 203

Query: 204 -NGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALLQKSIKVIDHFTYSVIATRKKEI 263
              F +AFD  N+  + RFIDP WK+K F N+GS++ L+KSI  ID F YS+I T++KE+
Sbjct: 204 GQEFMEAFDEGNVATSSRFIDPLWKLKWFFNIGSQSKLKKSIATIDKFVYSLITTKRKEL 263

Query: 264 QQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVVLNFVIAGRDTTAATLSWAIYMV 323
            +   T   ++ DILSRF+   E    N  +K LRD++LNF+IAG+DTTAA LSW +YM+
Sbjct: 264 AKEQNT--VVREDILSRFLVESEKDPENMNDKYLRDIILNFMIAGKDTTAALLSWFLYML 323

Query: 324 MTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQSFERRVTQFAELLNYDSLNRLH 383
             +  V +++  E++           ++  H   +D+         F E +N ++L+ +H
Sbjct: 324 CKNPLVQEKIVQEIRD----------VTFSHEKTTDVNG-------FVESINEEALDEMH 383

Query: 384 YLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGSDAT 443
           YLHA ++ETLRLYP VP D +    DDVLPDG +V  G  + Y+ Y+MGRM Y WG DA 
Sbjct: 384 YLHAALSETLRLYPPVPVDMRCAENDDVLPDGHRVSKGDNIYYIAYAMGRMTYIWGQDAE 443

Query: 444 SFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAYLQMKMALAILCRFYKFSLV-SG 503
            F P+RWLK  +F  ESPFKF +F AGPRIC+GKD AY QMK+    L  F++F +    
Sbjct: 444 EFKPERWLKDGLFQPESPFKFISFHAGPRICLGKDFAYRQMKIVSMALLHFFRFKMADEN 503

Query: 504 HEVSYRMMTILSMANGLKVTVARRS 527
            +V Y+ M  L +  GL +    R+
Sbjct: 504 SKVYYKRMLTLHVDGGLHLCAIPRT 509

BLAST of CmaCh05G008550 vs. TAIR 10
Match: AT2G44890.1 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 383.6 bits (984), Expect = 2.5e-106
Identity = 205/505 (40.59%), Postives = 295/505 (58.42%), Query Frame = 0

Query: 24  WMIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYI 83
           ++ I  +  +     +  P+     +   + H+++D+         T     P  +  + 
Sbjct: 18  YLTIRIFTGKSRNDKRYTPVHATIFDLFFHSHKLYDYETEIARTKPTFRFLSPGQSEIFT 77

Query: 84  ADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLR 143
           ADP NV+HILKT F NY KG      +  LLG GIF VDGE W++QRK  SFEF+++ LR
Sbjct: 78  ADPRNVEHILKTRFHNYSKGPVGTVNLADLLGHGIFAVDGEKWKQQRKLVSFEFSTRVLR 137

Query: 144 DFSALVFREYSLKLSDILTRASLHNQQVDIQELLMRMTLDSICKVGFGVEIGSLAPNLPE 203
           +FS  VFR  + KL   +   +L  +  D Q++LM+ TLDSI KVGFGVE+G L     E
Sbjct: 138 NFSYSVFRTSASKLVGFIAEFALSGKSFDFQDMLMKCTLDSIFKVGFGVELGCLDGFSKE 197

Query: 204 -NGFAKAFDTANMIVTLRFIDPFWKIKRFLNMGSEALLQKSIKVIDHFTYSVIATRKKEI 263
              F KAFD  N   + R  DPFWK+K FLN+GSE+ L+KSI +ID F YS+I T++KE+
Sbjct: 198 GEEFMKAFDEGNGATSSRVTDPFWKLKCFLNIGSESRLKKSIAIIDKFVYSLITTKRKEL 257

Query: 264 QQAPTTPNKIKHDILSRFIELGEDPNNNFTNKSLRDVVLNFVIAGRDTTAATLSWAIYMV 323
            +   T   ++ DILS+F+   E    N  +K LRD++LN ++AG+DTTAA+LSW +YM+
Sbjct: 258 SKEQNT--SVREDILSKFLLESEKDPENMNDKYLRDIILNVMVAGKDTTAASLSWFLYML 317

Query: 324 MTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQSFERRVTQFAELLNYDSLNRLH 383
             +  V +++  E++           ++  H   +D+  F   VT+       ++L ++ 
Sbjct: 318 CKNPLVQEKIVQEIRD----------VTSSHEKTTDVNGFIESVTE-------EALAQMQ 377

Query: 384 YLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGSDAT 443
           YLHA ++ET+RLYP VP+  +    DDVLPDG +V  G  + Y+ Y+MGRM Y WG DA 
Sbjct: 378 YLHAALSETMRLYPPVPEHMRCAENDDVLPDGHRVSKGDNIYYISYAMGRMTYIWGQDAE 437

Query: 444 SFNPQRWLKQDVFHNESPFKFTAFQAGPRICMGKDSAYLQMKMALAILCRFYKFSLV-SG 503
            F P+RWLK  VF  ES FKF +F AGPRIC+GKD AY QMK+    L  F++F +    
Sbjct: 438 EFKPERWLKDGVFQPESQFKFISFHAGPRICIGKDFAYRQMKIVSMALLHFFRFKMADEN 497

Query: 504 HEVSYRMMTILSMANGLKVTVARRS 527
            +VSY+ M  L +  GL +    R+
Sbjct: 498 SKVSYKKMLTLHVDGGLHLCAIPRT 503

BLAST of CmaCh05G008550 vs. TAIR 10
Match: AT4G00360.1 (cytochrome P450, family 86, subfamily A, polypeptide 2 )

HSP 1 Score: 373.2 bits (957), Expect = 3.3e-103
Identity = 210/532 (39.47%), Postives = 300/532 (56.39%), Query Frame = 0

Query: 9   SKSGVLGVATCLVWTW-MIIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNL 68
           S + +L       W W   I RW +    GP+ WP+LG+    +    RMHDW+   L  
Sbjct: 4   SNTMLLVAVVAAYWLWFQRISRWLK----GPRVWPVLGSLPGLIEQRDRMHDWITENLRA 63

Query: 69  S----KTVVVPMPFTT-----YTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGI 128
                +T +  +PF        T   DP N++H+LKT F NYPKG  + +     LG GI
Sbjct: 64  CGGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGI 123

Query: 129 FNVDGELWRKQRKTASFEFASKNLRD-FSALVFREYSLKLSDILTRASLHNQQVDIQELL 188
           FN DG+ W  QRKTA+ EF ++ LR      V R   L+   IL  A  + + VD+Q+L+
Sbjct: 124 FNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLI 183

Query: 189 MRMTLDSICKVGFGVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDP--FWKIKRFLNMG 248
           +R+T D+IC + FG +  + AP LPENGFA AFD A      RFI P   W++K++L +G
Sbjct: 184 LRLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLG 243

Query: 249 SEALLQKSIKVIDHFTYSVIATRKKEIQQAPTTPNKIKHDILSRFIELGEDPNNNFTNKS 308
            E  L +S+  ID +  +VI TRK+E+     +  +   D+LSRF+   +  + +++   
Sbjct: 244 LEVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFM---KKKDQSYSETF 303

Query: 309 LRDVVLNFVIAGRDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHH 368
           LR V LNF++AGRDT++  LSW  +++ TH  V D++  E+ S            L+   
Sbjct: 304 LRHVALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSV-----------LIETR 363

Query: 369 PSDIQSFERRVTQFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGT 428
            +D+ S+       AE L +D ++RL YL A ++ETLRLYP+VP+D K V+ DD+LPDGT
Sbjct: 364 GTDVSSWT------AEPLEFDEVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGT 423

Query: 429 KVRAGGMVTYVPYSMGRMEYNWGSDATSFNPQRWLKQD--VFHNESPFKFTAFQAGPRIC 488
            V AG  VTY  Y+ GRM+  WG D   F P+RW+  D   F N   ++F AF AGPRIC
Sbjct: 424 FVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRIC 483

Query: 489 MGKDSAYLQMKMALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARR 526
           +GKD AYLQMK   A +   ++ ++  GH+V  +M   L M NGL V V +R
Sbjct: 484 LGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFMKNGLLVNVHKR 511

BLAST of CmaCh05G008550 vs. TAIR 10
Match: AT1G01600.1 (cytochrome P450, family 86, subfamily A, polypeptide 4 )

HSP 1 Score: 367.9 bits (943), Expect = 1.4e-101
Identity = 209/520 (40.19%), Postives = 292/520 (56.15%), Query Frame = 0

Query: 22  WTWM-IIHRWNQRKNTGPKTWPLLGAALEQLLNYHRMHDWLLGYLNLS----KTVVVPMP 81
           W W   I RW +    GP+ WPLLG+    +    RMH+W+   L       +T +  +P
Sbjct: 17  WLWFKRISRWLK----GPRVWPLLGSLPGLIEQRDRMHEWITENLRACGGTYQTCIFAVP 76

Query: 82  FTT-----YTYIADPANVQHILKTNFPNYPKGDAYHSYMEVLLGDGIFNVDGELWRKQRK 141
           F        T   DP N++H+LKT F NYPKG  + S    LLG GIFN DG+ W  QRK
Sbjct: 77  FLAKKQGLVTVTCDPKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDTWLFQRK 136

Query: 142 TASFEFASKNLRD-FSALVFREYSLKLSDILTRASLHNQQVDIQELLMRMTLDSICKVGF 201
           TA+ EF ++ LR      V R   L+   IL  A  + + VD+Q+L++R+T D+IC + F
Sbjct: 137 TAALEFTTRTLRQAMGRWVNRGIKLRFCPILATAQDNAEPVDLQDLILRLTFDNICGLAF 196

Query: 202 GVEIGSLAPNLPENGFAKAFDTANMIVTLRFIDP--FWKIKRFLNMGSEALLQKSIKVID 261
           G +  + AP LPENGFA AFD A      RFI P   WK+K++L +G E  L +S+  ID
Sbjct: 197 GKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGLEVSLSRSLGEID 256

Query: 262 HFTYSVIATRKKEIQQAPTTPNKIKH-DILSRFIELGEDPNNNFTNKSLRDVVLNFVIAG 321
            +  +VI TRK+E+     +    +H D+LSRF+        ++++  L+ V LNF++AG
Sbjct: 257 EYLAAVINTRKQELMSQQESGTHQRHDDLLSRFM---MKKTESYSDTFLQHVALNFILAG 316

Query: 322 RDTTAATLSWAIYMVMTHSHVADRVYSELKSFEEERAKEEGISLLHHHPSDIQSFERRVT 381
           RDT++  LSW  +++  H  V D++  E+ S   E    + ++     P           
Sbjct: 317 RDTSSVALSWFFWLITMHPTVEDKIVREICSVLIETRGTDDVASWTEEP----------- 376

Query: 382 QFAELLNYDSLNRLHYLHAMITETLRLYPAVPQDPKGVLEDDVLPDGTKVRAGGMVTYVP 441
                L +D ++RL YL A I+ETLRLYP+VP+D K V  DDVLPDGT V AG  VTY  
Sbjct: 377 -----LGFDEIDRLVYLKAAISETLRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSI 436

Query: 442 YSMGRMEYNWGSDATSFNPQRWLK--QDVFHNESPFKFTAFQAGPRICMGKDSAYLQMKM 501
           Y+ GRM+  WG D   FNP+RW+      F N   ++F AF AGPRIC+GKD AYLQMK 
Sbjct: 437 YAAGRMKSTWGEDCLEFNPERWISPIDGKFINHDQYRFVAFNAGPRICLGKDLAYLQMKT 496

Query: 502 ALAILCRFYKFSLVSGHEVSYRMMTILSMANGLKVTVARR 526
             A +   ++ ++V GH+V  +M   L M NGL V + +R
Sbjct: 497 IAAAVLLRHRLTVVPGHKVEQKMSLTLFMKNGLLVNLYKR 513

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C7881.9e-22873.80Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1[more]
Q50EK31.0e-12547.08Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1[more]
O230664.7e-10239.47Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Q9LMM12.0e-10040.19Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
Q9CAD67.5e-10039.50Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ICQ42.0e-310100.00cytochrome P450 704B1 OS=Cucurbita maxima OX=3661 GN=LOC111471352 PE=3 SV=1[more]
A0A6J1G3781.9e-30899.24cytochrome P450 704B1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450376 P... [more]
A0A6J1DQX47.2e-27187.67cytochrome P450 704B1 OS=Momordica charantia OX=3673 GN=LOC111023497 PE=3 SV=1[more]
A0A1S3B4S22.7e-26283.67cytochrome P450 704B1 OS=Cucumis melo OX=3656 GN=LOC103485770 PE=3 SV=1[more]
A0A0A0LN462.4e-25882.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108690 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022972834.14.0e-310100.00cytochrome P450 704B1 [Cucurbita maxima][more]
XP_022946229.14.0e-30899.24cytochrome P450 704B1 isoform X1 [Cucurbita moschata][more]
XP_023545882.11.3e-30698.67cytochrome P450 704B1 [Cucurbita pepo subsp. pepo][more]
KAG7029959.11.7e-29899.41Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6598990.11.9e-29496.32Cytochrome P450 704B1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G69500.11.4e-22973.80cytochrome P450, family 704, subfamily B, polypeptide 1 [more]
AT2G45510.11.8e-10941.98cytochrome P450, family 704, subfamily A, polypeptide 2 [more]
AT2G44890.12.5e-10640.59cytochrome P450, family 704, subfamily A, polypeptide 1 [more]
AT4G00360.13.3e-10339.47cytochrome P450, family 86, subfamily A, polypeptide 2 [more]
AT1G01600.11.4e-10140.19cytochrome P450, family 86, subfamily A, polypeptide 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 295..312
score: 32.0
coord: 473..496
score: 21.09
coord: 385..403
score: 31.75
coord: 315..341
score: 21.5
coord: 463..473
score: 46.58
IPR001128Cytochrome P450PRINTSPR00385P450coord: 306..323
score: 39.37
coord: 473..484
score: 31.25
coord: 464..473
score: 48.47
coord: 386..397
score: 46.5
IPR001128Cytochrome P450PFAMPF00067p450coord: 37..518
e-value: 2.3E-66
score: 224.4
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 20..526
e-value: 1.8E-99
score: 335.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..525
NoneNo IPR availablePANTHERPTHR24296:SF8CYTOCHROME P450 704B1coord: 16..524
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 16..524

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G008550.1CmaCh05G008550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen