CmaCh05G007250 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh05G007250 vs. ExPASy Swiss-Prot
Match: Q91YK2 (Ribosomal RNA processing protein 1 homolog B OS=Mus musculus OX=10090 GN=Rrp1b PE=1 SV=2)

HSP 1 Score: 117.1 bits (292), Expect = 6.8e-25
Identity = 80/228 (35.09%), Postives = 120/228 (52.63%), Query Frame = 0

Query: 18  LASSNKATRDKTLRVIIKTWLPTQT-----SLSDGDMKKLWKGLFYCVWHADKAPVQSEL 77
           LASS K  RD+ +R  ++ +L  +T     S S  ++ K+WKGLFYC+W  D+  +Q EL
Sbjct: 16  LASSEKGVRDRAVRK-LRQYLSARTQSDTGSFSQEELLKIWKGLFYCMWVQDEPLLQEEL 75

Query: 78  INRLASLLLHLDLPLAVQYFSV--FLLTMRREWAGIDALRLDKFYLLIRRFLHQFFAFLK 137
            N + S L+H+   L  QY  +  F  TM REW GID L+LDK+Y+LIR  L Q F  LK
Sbjct: 76  AN-IISQLIHVVNSLEAQYLFIQTFWQTMNREWQGIDKLQLDKYYMLIRLVLRQSFEVLK 135

Query: 138 KHSWDLELCRRSVQVLEERVFFTDDKFHGSGVNYQIASVFLEELRPF--LPLQKDVIDIL 197
           +++W+       + +L + +   + +   +GV   +  V+LEEL       L  D    L
Sbjct: 136 RNAWEESQITLFLDILMKEILSPESQ-SPNGVRTHLIDVYLEELTTVGGAELLADQNLKL 195

Query: 198 FKPFIYSMAKLSDKVLLGKIKSNLFDVILKMG----KQLLERRKCGED 233
             PF    AK  D  L+  +   +F+VI+       ++ +E RK  ED
Sbjct: 196 IDPFCRIAAKTKDHTLVQTVARGVFEVIVDQSACVPQESVEERKTKED 240

BLAST of CmaCh05G007250 vs. ExPASy Swiss-Prot
Match: P56183 (Ribosomal RNA processing protein 1 homolog A OS=Mus musculus OX=10090 GN=Rrp1 PE=1 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 2.4e-22
Identity = 75/235 (31.91%), Postives = 114/235 (48.51%), Query Frame = 0

Query: 12  VSLVKLLASSNKATRDKTLRVIIKTWLPTQTSLSDG-----DMKKLWKGLFYCVWHADKA 71
           + L + LA + + TRD+ LR + K ++  ++  + G     ++ K+WKGLFYC+W  DK 
Sbjct: 11  IQLAQRLAGNEQVTRDRALRKLRK-YIEARSQRATGGFTPDELLKVWKGLFYCMWMQDK- 70

Query: 72  PVQSELINRLASLLLHL--DLPLAVQYFSVFLLTMRREWAGIDALRLDKFYLLIRRFLHQ 131
           P+Q E + R  + L+H         Q+   F  TM REW GID LRLDKFY+L+R  L +
Sbjct: 71  PLQQEELGRTIAQLVHAFHTTEAQHQFLKAFWQTMIREWVGIDRLRLDKFYMLMRMVLSE 130

Query: 132 FFAFLKKHSWDLELCRRSVQVLEERVFFTDDKFHGSGVNYQIASVFLEELRPF--LPLQK 191
               +K   WD     + +++L   +   D +   SGV      +FLEEL       L  
Sbjct: 131 SLKAVKARGWDERQIEQLLELLTTEILNPDSQ-APSGVKSHFLEIFLEELAKVGAAELTA 190

Query: 192 DVIDILFKPFIYSMAKLSDKVLLGKIKSNLFDVILKMGKQLLERRKCGEDVDSGD 238
           D       PF    A+  D  +L KI  ++F  I++     +E      D  SG+
Sbjct: 191 DQNLQFIDPFCQIAARTKDSQVLHKIIQSIFQTIVEQAPLAIEDIMNELDTQSGE 242

BLAST of CmaCh05G007250 vs. ExPASy Swiss-Prot
Match: Q14684 (Ribosomal RNA processing protein 1 homolog B OS=Homo sapiens OX=9606 GN=RRP1B PE=1 SV=3)

HSP 1 Score: 107.8 bits (268), Expect = 4.1e-22
Identity = 72/214 (33.64%), Postives = 108/214 (50.47%), Query Frame = 0

Query: 12  VSLVKLLASSNKATRDKTLR-----VIIKTWLPTQTSLSDGDMKKLWKGLFYCVWHADKA 71
           +   + LASS K  RD+ ++     + +KT   T    S  ++ K+WKGLFYC+W  D+ 
Sbjct: 10  IQFAQRLASSEKGIRDRAVKKLRQYISVKTQRET-GGFSQEELLKIWKGLFYCMWVQDEP 69

Query: 72  PVQSELINRLASLLLHLDLPLAVQYF-SVFLLTMRREWAGIDALRLDKFYLLIRRFLHQF 131
            +Q EL N +A L+  ++   A   F   F  TM REW GID LRLDK+Y+LIR  L Q 
Sbjct: 70  LLQEELANTIAQLVHAVNNSAAQHLFIQTFWQTMNREWKGIDRLRLDKYYMLIRLVLRQS 129

Query: 132 FAFLKKHSWDLELCRRSVQVLEERVFFTDDKFHGSGVNYQIASVFLEELRPF--LPLQKD 191
           F  LK++ W+    +  + VL + V   + +   +GV +    ++L+EL       L  D
Sbjct: 130 FEVLKRNGWEESRIKVFLDVLMKEVLCPESQ-SPNGVRFHFIDIYLDELSKVGGKELLAD 189

Query: 192 VIDILFKPFIYSMAKLSDKVLLGKIKSNLFDVIL 218
                  PF    AK  D  L+  I   +F+ I+
Sbjct: 190 QNLKFIDPFCKIAAKTKDHTLVQTIARGVFEAIV 221

BLAST of CmaCh05G007250 vs. ExPASy Swiss-Prot
Match: P56182 (Ribosomal RNA processing protein 1 homolog A OS=Homo sapiens OX=9606 GN=RRP1 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.0e-20
Identity = 70/223 (31.39%), Postives = 108/223 (48.43%), Query Frame = 0

Query: 12  VSLVKLLASSNKATRDKTLRVIIKTWLPTQTSLSDG-----DMKKLWKGLFYCVWHADKA 71
           + L + LA + + TRD+ +R + K ++  +T  + G     ++ K+WKGLFYC+W  DK 
Sbjct: 11  IQLAQRLAGNEQVTRDRAVRKLRK-YIVARTQRAAGGFTHDELLKVWKGLFYCMWMQDKP 70

Query: 72  PVQSELINRLASLLLHLDLPLAVQ--YFSVFLLTMRREWAGIDALRLDKFYLLIRRFLHQ 131
            +Q EL  R  S L+H       Q  +   F  TM REW GID LRLDKFY+L+R  L++
Sbjct: 71  LLQEEL-GRTISQLVHAFQTTEAQHLFLQAFWQTMNREWTGIDRLRLDKFYMLMRMVLNE 130

Query: 132 FFAFLKKHSWDLELCRRSVQVLEERVFFTDDKFHGSGVNYQIASVFLEELRPF--LPLQK 191
               LK   W+       +++L   +     +   +GV      +FLEEL       L  
Sbjct: 131 SLKVLKMQGWEERQIEELLELLMTEILHPSSQ-APNGVKSHFIEIFLEELTKVGAEELTA 190

Query: 192 DVIDILFKPFIYSMAKLSDKVLLGKIKSNLFDVILKMGKQLLE 226
           D       PF    A+  D ++L  I   +F+ I++     +E
Sbjct: 191 DQNLKFIDPFCRIAARTKDSLVLNNITRGIFETIVEQAPLAIE 230

BLAST of CmaCh05G007250 vs. ExPASy Swiss-Prot
Match: Q9Y7M6 (Ribosomal RNA-processing protein 1 homolog OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC9B6.07 PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.1e-20
Identity = 72/210 (34.29%), Postives = 109/210 (51.90%), Query Frame = 0

Query: 15  VKLLASSNKATRDKTLRVIIKTWLPTQTSLSDGDMKKLWKGLFYCVWHADKAPVQSELIN 74
           +K LA++++ TRDK L   ++ +L  +      D  KLWKGLFYC+W ADK   Q +L +
Sbjct: 8   IKKLAANDRKTRDKALE-SLQRFLSQKKKFERLDFLKLWKGLFYCMWMADKPLYQQKLSD 67

Query: 75  RLASLLLHLDLPLAVQYFSVFLLTMRREWAGIDALRLDKFYLLIRRFLHQFFAFLKKHSW 134
            LA+L+  + +   + + S F  TM REW GID LR DKFYLL+RRF    F  ++  S 
Sbjct: 68  NLAALVPIVWIDNRILFQSTFWETMGREWTGIDILRTDKFYLLMRRFCAAAFRDIQTRSK 127

Query: 135 DL---ELCRRSVQVLEERVFFTDDKFHGSGVNYQIASVFLEELRPFLPLQKDVIDILFKP 194
                ++     Q+  +  F T++    +G+ + +A ++ EELR   P      D  + P
Sbjct: 128 TALLDKVVAEYNQMWMDGPFNTENLAFPNGILFHLADIWTEELRKVYPEDVPKAD-WYLP 187

Query: 195 FIYSMAKLSDKVLLGKIKSNLFDVILKMGK 222
           F  S  K S  V+L K      D + +  K
Sbjct: 188 F-DSTIKSSHNVVLRKTLPKRLDRVSEYTK 214

BLAST of CmaCh05G007250 vs. TAIR 10
Match: AT5G20600.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: rRNA processing; LOCATED IN: preribosome, small subunit precursor; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Nucleolar, Nop52 (InterPro:IPR010301); Has 543 Blast hits to 530 proteins in 201 species: Archae - 0; Bacteria - 10; Metazoa - 211; Fungi - 164; Plants - 46; Viruses - 0; Other Eukaryotes - 112 (source: NCBI BLink). )

HSP 1 Score: 443.4 bits (1139), Expect = 3.0e-124
Identity = 269/575 (46.78%), Postives = 360/575 (62.61%), Query Frame = 0

Query: 13  SLVKLLASSNKATRDKTLRVIIKTWLPTQTSLSDGDMKKLWKGLFYCVWHADKAPVQSEL 72
           SL+K LAS  ++ RD++LR +++TWLP QT +SD DMKKLW+G+FYCVWHADK+  QSEL
Sbjct: 22  SLIKKLASCKQSIRDRSLRTVLRTWLPEQTEISDEDMKKLWQGIFYCVWHADKSLYQSEL 81

Query: 73  INRLASLLLHLDLPLAVQYFSVFLLTMRREWAGIDALRLDKFYLLIRRFLHQFFAFLKKH 132
           I+RL+S L  L LPL++ YF+VFL TMRREW+ ID LRLDKFYLLIRRFLH FF+ +  +
Sbjct: 82  IDRLSSALQSLPLPLSIHYFTVFLFTMRREWSRIDRLRLDKFYLLIRRFLHGFFSLIDSN 141

Query: 133 SWDLELCRRSVQVLEERVFFTDDKFHGSGVNYQIASVFLEELRPFLPLQKDVIDILFKPF 192
           SWDLE  RR + +L +     DDKF G+GVNY I S F+EELRPFLPL+K+V+++LF PF
Sbjct: 142 SWDLEFTRRLMGILFDGTLLADDKFQGNGVNYHITSAFVEELRPFLPLRKEVLELLFAPF 201

Query: 193 IYSMAKLSDKVLLGKIKSNLFDVILKMGKQLLERRKCGED-VDSGDDMVVLGSISLTNLF 252
           +  M  ++DK+L+GKIKSNLFD +LKMGK+LL  ++ G+D V   DD+VVLGSI+L+  F
Sbjct: 202 VKIMGVVNDKILVGKIKSNLFDELLKMGKKLLIIKQSGDDAVPEDDDVVVLGSIALSMEF 261

Query: 253 STTFYELGSSPDCCQGNRKVLFSMHEEYQKLEKDIISSGIEISFPDLQEQDVNEVPTLVP 312
           +  FYELGSSP+C QGNRK++F++H+E+ +LEKD+ SS IE+S  +      +EVP  VP
Sbjct: 262 AKRFYELGSSPECHQGNRKLIFALHKEFLQLEKDMSSSSIEVSIVEASRPGNDEVPESVP 321

Query: 313 VSSEGEAPSEISVVDVDITAESTDKAHKKKKKNKKATPSEISMLDVDLTAESTDKPLKKQ 372
           VS      S  +VV                 K+KK   +  S         S  K LKK 
Sbjct: 322 VSE-----SNTNVV----------------SKSKKIVANGSS---------SKKKGLKKC 381

Query: 373 KKNKKATDGSGKKKTKKTTNGSSDLVSENTTVNKDNENIVASNGENSNNEQISDGNMIAL 432
            K KK +   G         G           NKD                       + 
Sbjct: 382 SKTKKDSVKKGAVMDSVVDLG-----------NKDE----------------------SF 441

Query: 433 DESVISNLQMQFERVASEAEFGVNVVSPV--VSTNGSVKKRKRAKNVDGLQKHDVVLPSE 492
           DE++IS+L+ +FE++ASE     + V+ V  V+   SV K+ + +  +   +  V   + 
Sbjct: 442 DETMISDLRQEFEKIASEMSPSKSEVASVCDVAEPVSVSKKTKKRKRENKGEEQVSTVNR 501

Query: 493 VDAEGNTAVKSGEKSVKKVRFSMKNNLVWKPHNPLPPESLRLPPSVTPRGSALKKGIPPG 552
             AE + A  S EKS K+VRF+MKNNLVWKPH PLPP+ LR PPS TPRGSALK+GIP G
Sbjct: 502 DAAETSLAGPSSEKSSKRVRFAMKNNLVWKPHCPLPPQDLRCPPSATPRGSALKQGIPAG 528

Query: 553 PILEFPATAKRAKRRAVVMRAKKGVRSLPVKRVKK 585
           P+ E    A R  ++ V   A++GV+S  +K +KK
Sbjct: 562 PVREL---ASRKTKKKVKPAARRGVKS--IKNLKK 528