CmaCh05G006480 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G006480
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiongibberellin 3-beta-dioxygenase 2-like
LocationCma_Chr05: 3289026 .. 3290688 (+)
RNA-Seq ExpressionCmaCh05G006480
SyntenyCmaCh05G006480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCCAAAACTCTAAAACAAACAAACCCATTTCTTTGCTTTGCCAATCCATCAACATAATGACCACTCTCTCTCAAGCATATAGAGACCACCCTCTTATTCACCTTCACCATATCGTCCCTTTGGACTTCGATTCATTACGAACCGTCCCTGACTCTCACGATTGGCCTCATTCTTCGTACGATACCCTTAAACTTCATACTAATGGAGATGTTTCGATTCCCTTGATCGATATGTCTGACCCTAATGTAGCGATGCTCATTGGTAATGTATGTAAGACATGGGGAGTGTTCCAATTGACCAACCATGGCGTGCCAACGAGTCTGGTGGAGAGAGCTGAAGCTGAGGCGGGACGACTATTCGATCTTCCAATGACACGAAAACTAAAGGCGCTTCGAGCTCCCGGCGACGCCACCGGCTACGGCTTGCCTCGAATAACGCCATTTTTCTCTAAGTATATGTGGCATGAGGGATTTACTATTATGGGATCCTCCGCAGAACATGCTTCTCAACTTTGGTCTTCTAACTACCAACCGTTCTGGTAACTACTTCTTCATGCATTTTCATACACGTTTCTATTTATTTATTTTCTTTTCTTTTCTTTTCTTTCTTTGCTATTTTAACTGCTGGAATTAATTTATATATCCTAAATATATCTACTTTAGATATAATATAAAAAATGTGAAATATAATTACACTGTTTTTTTTGGTTATATTACAAATTTATTTTTATAAAATCGTACAAAAAAAATTAATAAATGATAAAAAATTTAATATTTAATTTTGTATTTAATAAATTCATAATTACTGACTAACGTTAAAGCTAAAAAAAAATAATGTCTAATTAAGTTCGAAAAAAGAAAATTAAAAATTTAGATATCTAATTATTTTAAAATTTTAATAAATACTTTTAAAATTAAGAAATTGATTAGACACAAAGCATTTGAAGATTGTTCTTCGAGTAATTTAATATAATCTATACAATTTATTATTATTATTATACGATGATAATTGATATTTTAATCACTTTTTATTATTATTTTTTTTTTAATAATCACTTATTAACTCTATTAATTAATATTCAAATGAAGCGATGTAATGGAAGAGTACCAGAAGAACATGAAGTCCTTAGCTGAGAAAATCACACATTTAATCTTAAATTTCCTTAAAATCCCCGACGACGCCGAAATCTCGAACTGGCTCCATTCCGCCGGAGCCGCTGCCGCCTGCAGCACCGCCTTGCAATTAAACTGCTACCCCCGGTGCCCGGATCCAACCCGGGTCATGGGTCTGGCCCCACATACCGACACCTTTCTATTGACAATCCTCCACCAAAGCAGCACGTGCGGGCTCCAAATCTTCAGGGACGGGTTCGGCTGGCTACCTGTGACGCCGGTAGCCGGTGCACTAGTCCTCAACGTCGGCGACCTGTTCCACATCCTGTCGAATGGGCGGTTCCCGAATGTCCTCCACCGGGTTGTCGTCGACCCGACCCGACGCCGCCTGTCTATGGCGTATTTCTTTGGACCGCCGCCGGATTTCTGCGTGACGCCGTTTAACAATCCACCGGAATCTCCCCGGTTCCGGTCTGTGGTGGTGAAGGAGTATGTCGGGTTGAAAGCGAAGAATCTGGAGTGTGCCCTCTCTATGATCGAAATATAA

mRNA sequence

ATGCTCCAAAACTCTAAAACAAACAAACCCATTTCTTTGCTTTGCCAATCCATCAACATAATGACCACTCTCTCTCAAGCATATAGAGACCACCCTCTTATTCACCTTCACCATATCGTCCCTTTGGACTTCGATTCATTACGAACCGTCCCTGACTCTCACGATTGGCCTCATTCTTCGTACGATACCCTTAAACTTCATACTAATGGAGATGTTTCGATTCCCTTGATCGATATGTCTGACCCTAATGTAGCGATGCTCATTGGTAATGTATGTAAGACATGGGGAGTGTTCCAATTGACCAACCATGGCGTGCCAACGAGTCTGGTGGAGAGAGCTGAAGCTGAGGCGGGACGACTATTCGATCTTCCAATGACACGAAAACTAAAGGCGCTTCGAGCTCCCGGCGACGCCACCGGCTACGGCTTGCCTCGAATAACGCCATTTTTCTCTAAGTATATGTGGCATGAGGGATTTACTATTATGGGATCCTCCGCAGAACATGCTTCTCAACTTTGGTCTTCTAACTACCAACCGTTCTGCGATGTAATGGAAGAGTACCAGAAGAACATGAAGTCCTTAGCTGAGAAAATCACACATTTAATCTTAAATTTCCTTAAAATCCCCGACGACGCCGAAATCTCGAACTGGCTCCATTCCGCCGGAGCCGCTGCCGCCTGCAGCACCGCCTTGCAATTAAACTGCTACCCCCGGTGCCCGGATCCAACCCGGGTCATGGGTCTGGCCCCACATACCGACACCTTTCTATTGACAATCCTCCACCAAAGCAGCACGTGCGGGCTCCAAATCTTCAGGGACGGGTTCGGCTGGCTACCTGTGACGCCGGTAGCCGGTGCACTAGTCCTCAACGTCGGCGACCTGTTCCACATCCTGTCGAATGGGCGGTTCCCGAATGTCCTCCACCGGGTTGTCGTCGACCCGACCCGACGCCGCCTGTCTATGGCGTATTTCTTTGGACCGCCGCCGGATTTCTGCGTGACGCCGTTTAACAATCCACCGGAATCTCCCCGGTTCCGGTCTGTGGTGGTGAAGGAGTATGTCGGGTTGAAAGCGAAGAATCTGGAGTGTGCCCTCTCTATGATCGAAATATAA

Coding sequence (CDS)

ATGCTCCAAAACTCTAAAACAAACAAACCCATTTCTTTGCTTTGCCAATCCATCAACATAATGACCACTCTCTCTCAAGCATATAGAGACCACCCTCTTATTCACCTTCACCATATCGTCCCTTTGGACTTCGATTCATTACGAACCGTCCCTGACTCTCACGATTGGCCTCATTCTTCGTACGATACCCTTAAACTTCATACTAATGGAGATGTTTCGATTCCCTTGATCGATATGTCTGACCCTAATGTAGCGATGCTCATTGGTAATGTATGTAAGACATGGGGAGTGTTCCAATTGACCAACCATGGCGTGCCAACGAGTCTGGTGGAGAGAGCTGAAGCTGAGGCGGGACGACTATTCGATCTTCCAATGACACGAAAACTAAAGGCGCTTCGAGCTCCCGGCGACGCCACCGGCTACGGCTTGCCTCGAATAACGCCATTTTTCTCTAAGTATATGTGGCATGAGGGATTTACTATTATGGGATCCTCCGCAGAACATGCTTCTCAACTTTGGTCTTCTAACTACCAACCGTTCTGCGATGTAATGGAAGAGTACCAGAAGAACATGAAGTCCTTAGCTGAGAAAATCACACATTTAATCTTAAATTTCCTTAAAATCCCCGACGACGCCGAAATCTCGAACTGGCTCCATTCCGCCGGAGCCGCTGCCGCCTGCAGCACCGCCTTGCAATTAAACTGCTACCCCCGGTGCCCGGATCCAACCCGGGTCATGGGTCTGGCCCCACATACCGACACCTTTCTATTGACAATCCTCCACCAAAGCAGCACGTGCGGGCTCCAAATCTTCAGGGACGGGTTCGGCTGGCTACCTGTGACGCCGGTAGCCGGTGCACTAGTCCTCAACGTCGGCGACCTGTTCCACATCCTGTCGAATGGGCGGTTCCCGAATGTCCTCCACCGGGTTGTCGTCGACCCGACCCGACGCCGCCTGTCTATGGCGTATTTCTTTGGACCGCCGCCGGATTTCTGCGTGACGCCGTTTAACAATCCACCGGAATCTCCCCGGTTCCGGTCTGTGGTGGTGAAGGAGTATGTCGGGTTGAAAGCGAAGAATCTGGAGTGTGCCCTCTCTATGATCGAAATATAA

Protein sequence

MLQNSKTNKPISLLCQSINIMTTLSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSSYDTLKLHTNGDVSIPLIDMSDPNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATGYGLPRITPFFSKYMWHEGFTIMGSSAEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKITHLILNFLKIPDDAEISNWLHSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILHQSSTCGLQIFRDGFGWLPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFFGPPPDFCVTPFNNPPESPRFRSVVVKEYVGLKAKNLECALSMIEI
Homology
[Close]
BLAST of CmaCh05G006480 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.6e-91
Identity = 168/357 (47.06%), Postives = 230/357 (64.43%), Query Frame = 0 

Query: 21  MTTLSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSSYDTL----KLHTNGDVSIPL 80
           M +LS+AYR HP +H++H  P DF+SL+ +P+S++W H    TL     +      ++P+
Sbjct: 1   MPSLSEAYRAHP-VHVNHKHP-DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPV 60

Query: 81  IDMSDPNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPG 140
           ID++DPN + LIG  CKTWGV+Q+ NHG+P SL+E  +     LF LP  +K KA R+P 
Sbjct: 61  IDLNDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPD 120

Query: 141 DATGYGLPRITPFFSKYMWHEGFTIMGSSAEHASQLWSSNYQPFCDVMEEYQKNMKSLAE 200
             +GYG+ RI+ FF K MW+EGFTI+GS  +H  +LW  +Y  FCD++ +Y + MK LA 
Sbjct: 121 GVSGYGIARISSFFPKLMWYEGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAG 180

Query: 201 KITHLILNFLKIPDDAEISNWLHSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 260
            +  L+L+ L I    E   W  S         ALQLN YP CPDP   MGLAPHTD+  
Sbjct: 181 TLMCLMLDSLGI--TKEDIKWAGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTF 240

Query: 261 LTILHQSSTCGLQIFRDGFGWLPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 320
           LTIL Q+   GLQ+ R+G GW+ V P+ G LV+NVGDLFHILSNG +P+VLHRV+V+ TR
Sbjct: 241 LTILSQNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTR 300

Query: 321 RRLSMAYFFGPPPDFCVTP---FNNPPESPRFRSVVVKEYVGLKAKNLECALSMIEI 371
           +R S+AY +GPP +  + P      P + P +RSV   EY+G KAK+   ALS + +
Sbjct: 301 QRFSVAYLYGPPSNVEICPHAKLIGPTKPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

[Close]
BLAST of CmaCh05G006480 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 319.3 bits (817), Expect = 5.7e-86
Identity = 165/351 (47.01%), Postives = 219/351 (62.39%), Query Frame = 0 

Query: 24  LSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSS---YDTLKLHTNGDVSIPLIDMS 83
           L+  +R HP IHL H    DF SLR +PDS+ W       +           +IPLID+ 
Sbjct: 5   LTDVFRGHP-IHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLD 64

Query: 84  DPNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATG 143
            P+    IG+ C+TWG FQ++NHGVP  L++  E   G LF LP+ RKLK+ R+    +G
Sbjct: 65  HPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSG 124

Query: 144 YGLPRITPFFSKYMWHEGFTIMGSSAEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKITH 203
           YG+ RI  FF+K MW EGFTI GS      +LW  ++  +CD++EEY+++MK LA K+  
Sbjct: 125 YGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMW 184

Query: 204 LILNFLKIPDDAEISNWLHSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTIL 263
           L LN L + +  E   W   +        ALQLN YP CP+P R MGLA HTD+ LLTIL
Sbjct: 185 LALNSLGVSE--EDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTIL 244

Query: 264 HQSSTCGLQIFRDGFGWLPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLS 323
           +Q++T GLQ+FRD  GW+ V P  G+LV+NVGDLFHILSNG F +VLHR  V+ TR RLS
Sbjct: 245 YQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLS 304

Query: 324 MAYFFGPPPDFCVTP---FNNPPESPRFRSVVVKEYVGLKAKNLECALSMI 369
           +A+ +GP  D  ++P     +P ESP ++SV  KEY+  KA +   ALSMI
Sbjct: 305 VAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMI 352

[Close]
BLAST of CmaCh05G006480 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 315.8 bits (808), Expect = 6.3e-85
Identity = 160/350 (45.71%), Postives = 225/350 (64.29%), Query Frame = 0 

Query: 22  TTLSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSSYDTLKLHTNGDVSIPLIDMSD 81
           +TLS  +R HP IH+    P DF SL   PDS+ W     D L   +  D ++PLID+SD
Sbjct: 3   STLSDVFRSHP-IHIPLSNPPDFKSL---PDSYTWTPKD-DLLFSASASDETLPLIDLSD 62

Query: 82  PNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATGY 141
            +VA L+G+ C TWG FQ+TNHGVP+ L++  E   G LF LP+ RKLKA R+    +GY
Sbjct: 63  IHVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGY 122

Query: 142 GLPRITPFFSKYMWHEGFTIMGSSAEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKITHL 201
           G+ RI  FF+K MW EGFT++GS      +LW S++  +C+++EEY+++M+ LA K+   
Sbjct: 123 GVARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWF 182

Query: 202 ILNFLKIPDDAEISNWLHSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILH 261
            L  L + ++ +I  W             +QLN YP+CP+P R MGLA HTD+ L+TIL+
Sbjct: 183 ALGSLGV-EEKDI-QWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILY 242

Query: 262 QSSTCGLQIFRDGFGWLPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSM 321
           Q++T GLQ+FRD  GW+   PV G+LV+NVGDL HIL+NG FP+VLHR  V+  R R SM
Sbjct: 243 QNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSM 302

Query: 322 AYFFGPPPDFCVTPFN---NPPESPRFRSVVVKEYVGLKAKNLECALSMI 369
           AY +GPP D  ++P     +P +SP + S+  K+Y+  KA +   +LS+I
Sbjct: 303 AYLWGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSII 345

[Close]
BLAST of CmaCh05G006480 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.5e-75
Identity = 146/355 (41.13%), Postives = 223/355 (62.82%), Query Frame = 0 

Query: 21  MTTLSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSSYDTLKLHTNGDVSIPLIDMS 80
           M++++Q ++++P ++   I+PLDF + +T+PDSH W     +T    T+G   IP+I +S
Sbjct: 1   MSSVTQLFKNNP-VNRDRIIPLDFTNTKTLPDSHVWSKPEPET----TSG--PIPVISLS 60

Query: 81  DPNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATG 140
           +P    L+   C+ WGVF +T+HGV  SL+   + +  RLF LPM RK+ A+R+P ++TG
Sbjct: 61  NPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTG 120

Query: 141 YGLPRITPFFSKYMWHEGFTIMGSS-AEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKIT 200
           YG+ RI+ F+ K MW EGF++MGSS   HA+ LW  ++  FC+VMEEYQK M  L+ ++ 
Sbjct: 121 YGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLI 180

Query: 201 HLILNFLKIPDDAEISNWL---HSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 260
            +++  L +    E   WL    +     +  + LQLN YP CPDP   MGLAPHTD+ L
Sbjct: 181 SMLMGSLGLTH--EDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSL 240

Query: 261 LTILHQSSTCGLQI---FRDGFGWLPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVD 320
           LTIL+Q +  GL+I     +G  W+ V P+ G+LV+ +GDL HI+SNG+F + +HR VV+
Sbjct: 241 LTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVN 300

Query: 321 PTRRRLSMAYFFGPPPDFCVTPFNNPPESPR-FRSVVVKEYVGLKAKNLECALSM 368
            T  R+S AYF GPP +  + P  +    P  +R ++ +EY+  KA +   AL++
Sbjct: 301 KTHHRVSAAYFAGPPKNLQIGPLTSDKNHPPIYRRLIWEEYLAAKATHFNKALTL 346

[Close]
BLAST of CmaCh05G006480 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.4e-75
Identity = 144/337 (42.73%), Postives = 214/337 (63.50%), Query Frame = 0 

Query: 46  SLRTVPDSHDWPHSSYDTLKLHTNG------DVSIPLIDMSDPNVAMLIGNVCKTWGVFQ 105
           SL+T+P+S  W  ++ D+L   ++       + SIP+ID+S+P+V  LIG+  KTWG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 106 LTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATGYGLPRITPFFSKYMWHEGF 165
           + NHG+   L++  E+ +  LFD+P  RKL+A  +    +GYG PRI+PFF K MW EGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 166 TIMGSS-AEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKITHLILNFLKIP-DDAEISNW 225
           TI   S   H + LW  ++  +C +++EY   M+ LA ++ + IL  L +  +D E ++ 
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194

Query: 226 LHSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILHQSSTCGLQIFRDGFGW 285
           L  +G+      A++LN YP CP+P R MGLA HTD+ +LTILHQS+T GLQ+FR+  GW
Sbjct: 195 LEKSGSKVG-RGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGW 254

Query: 286 LPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFF-GPPPDFCVTPF 345
           + V P  G LV+N+GDLFHILSNG+ P+V+HR  V+ TR R+S+AY + GP  D  + P 
Sbjct: 255 VTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPI 314

Query: 346 N---NPPESPRFRSVVVKEYVGLKAKNLECALSMIEI 371
           +    P E   +RS+  KEY+ +K +  + A+  I +
Sbjct: 315 SKLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]
BLAST of CmaCh05G006480 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 319.3 bits (817), Expect = 4.0e-87
Identity = 165/351 (47.01%), Postives = 219/351 (62.39%), Query Frame = 0 

Query: 24  LSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSS---YDTLKLHTNGDVSIPLIDMS 83
           L+  +R HP IHL H    DF SLR +PDS+ W       +           +IPLID+ 
Sbjct: 5   LTDVFRGHP-IHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLD 64

Query: 84  DPNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATG 143
            P+    IG+ C+TWG FQ++NHGVP  L++  E   G LF LP+ RKLK+ R+    +G
Sbjct: 65  HPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSG 124

Query: 144 YGLPRITPFFSKYMWHEGFTIMGSSAEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKITH 203
           YG+ RI  FF+K MW EGFTI GS      +LW  ++  +CD++EEY+++MK LA K+  
Sbjct: 125 YGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMW 184

Query: 204 LILNFLKIPDDAEISNWLHSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTIL 263
           L LN L + +  E   W   +        ALQLN YP CP+P R MGLA HTD+ LLTIL
Sbjct: 185 LALNSLGVSE--EDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTIL 244

Query: 264 HQSSTCGLQIFRDGFGWLPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLS 323
           +Q++T GLQ+FRD  GW+ V P  G+LV+NVGDLFHILSNG F +VLHR  V+ TR RLS
Sbjct: 245 YQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLS 304

Query: 324 MAYFFGPPPDFCVTP---FNNPPESPRFRSVVVKEYVGLKAKNLECALSMI 369
           +A+ +GP  D  ++P     +P ESP ++SV  KEY+  KA +   ALSMI
Sbjct: 305 VAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMI 352

[Close]
BLAST of CmaCh05G006480 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 315.8 bits (808), Expect = 4.5e-86
Identity = 160/350 (45.71%), Postives = 225/350 (64.29%), Query Frame = 0 

Query: 22  TTLSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSSYDTLKLHTNGDVSIPLIDMSD 81
           +TLS  +R HP IH+    P DF SL   PDS+ W     D L   +  D ++PLID+SD
Sbjct: 3   STLSDVFRSHP-IHIPLSNPPDFKSL---PDSYTWTPKD-DLLFSASASDETLPLIDLSD 62

Query: 82  PNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATGY 141
            +VA L+G+ C TWG FQ+TNHGVP+ L++  E   G LF LP+ RKLKA R+    +GY
Sbjct: 63  IHVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGY 122

Query: 142 GLPRITPFFSKYMWHEGFTIMGSSAEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKITHL 201
           G+ RI  FF+K MW EGFT++GS      +LW S++  +C+++EEY+++M+ LA K+   
Sbjct: 123 GVARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWF 182

Query: 202 ILNFLKIPDDAEISNWLHSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILH 261
            L  L + ++ +I  W             +QLN YP+CP+P R MGLA HTD+ L+TIL+
Sbjct: 183 ALGSLGV-EEKDI-QWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILY 242

Query: 262 QSSTCGLQIFRDGFGWLPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSM 321
           Q++T GLQ+FRD  GW+   PV G+LV+NVGDL HIL+NG FP+VLHR  V+  R R SM
Sbjct: 243 QNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSM 302

Query: 322 AYFFGPPPDFCVTPFN---NPPESPRFRSVVVKEYVGLKAKNLECALSMI 369
           AY +GPP D  ++P     +P +SP + S+  K+Y+  KA +   +LS+I
Sbjct: 303 AYLWGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSII 345

[Close]
BLAST of CmaCh05G006480 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 284.6 bits (727), Expect = 1.1e-76
Identity = 146/355 (41.13%), Postives = 223/355 (62.82%), Query Frame = 0 

Query: 21  MTTLSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSSYDTLKLHTNGDVSIPLIDMS 80
           M++++Q ++++P ++   I+PLDF + +T+PDSH W     +T    T+G   IP+I +S
Sbjct: 1   MSSVTQLFKNNP-VNRDRIIPLDFTNTKTLPDSHVWSKPEPET----TSG--PIPVISLS 60

Query: 81  DPNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATG 140
           +P    L+   C+ WGVF +T+HGV  SL+   + +  RLF LPM RK+ A+R+P ++TG
Sbjct: 61  NPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTG 120

Query: 141 YGLPRITPFFSKYMWHEGFTIMGSS-AEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKIT 200
           YG+ RI+ F+ K MW EGF++MGSS   HA+ LW  ++  FC+VMEEYQK M  L+ ++ 
Sbjct: 121 YGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLI 180

Query: 201 HLILNFLKIPDDAEISNWL---HSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 260
            +++  L +    E   WL    +     +  + LQLN YP CPDP   MGLAPHTD+ L
Sbjct: 181 SMLMGSLGLTH--EDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSL 240

Query: 261 LTILHQSSTCGLQI---FRDGFGWLPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVD 320
           LTIL+Q +  GL+I     +G  W+ V P+ G+LV+ +GDL HI+SNG+F + +HR VV+
Sbjct: 241 LTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVN 300

Query: 321 PTRRRLSMAYFFGPPPDFCVTPFNNPPESPR-FRSVVVKEYVGLKAKNLECALSM 368
            T  R+S AYF GPP +  + P  +    P  +R ++ +EY+  KA +   AL++
Sbjct: 301 KTHHRVSAAYFAGPPKNLQIGPLTSDKNHPPIYRRLIWEEYLAAKATHFNKALTL 346

[Close]
BLAST of CmaCh05G006480 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 283.5 bits (724), Expect = 2.5e-76
Identity = 144/337 (42.73%), Postives = 214/337 (63.50%), Query Frame = 0 

Query: 46  SLRTVPDSHDWPHSSYDTLKLHTNG------DVSIPLIDMSDPNVAMLIGNVCKTWGVFQ 105
           SL+T+P+S  W  ++ D+L   ++       + SIP+ID+S+P+V  LIG+  KTWG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 106 LTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATGYGLPRITPFFSKYMWHEGF 165
           + NHG+   L++  E+ +  LFD+P  RKL+A  +    +GYG PRI+PFF K MW EGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 166 TIMGSS-AEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKITHLILNFLKIP-DDAEISNW 225
           TI   S   H + LW  ++  +C +++EY   M+ LA ++ + IL  L +  +D E ++ 
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194

Query: 226 LHSAGAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILHQSSTCGLQIFRDGFGW 285
           L  +G+      A++LN YP CP+P R MGLA HTD+ +LTILHQS+T GLQ+FR+  GW
Sbjct: 195 LEKSGSKVG-RGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGW 254

Query: 286 LPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFF-GPPPDFCVTPF 345
           + V P  G LV+N+GDLFHILSNG+ P+V+HR  V+ TR R+S+AY + GP  D  + P 
Sbjct: 255 VTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPI 314

Query: 346 N---NPPESPRFRSVVVKEYVGLKAKNLECALSMIEI 371
           +    P E   +RS+  KEY+ +K +  + A+  I +
Sbjct: 315 SKLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]
BLAST of CmaCh05G006480 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 5.3e-39
Identity = 98/332 (29.52%), Postives = 165/332 (49.70%), Query Frame = 0 

Query: 49  TVPDSHDWP-HSSYDTLKLHTNGDVSIPLIDMSD----PNVAMLIGNVCKTWGVFQLTNH 108
           TVP+ +  P H         ++  + IP++DM+D    P    L+ + C+ WG FQ+ NH
Sbjct: 22  TVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLRLVRSACEEWGFFQMVNH 81

Query: 109 GVPTSLVERAEAEAGRLFDLPMTRKLKALRAPGDATGYGLPRITPFFSKYMWHEGFTI-- 168
           GV  SL+ER        F+LP+  K K   +P    GYG        +K  W + F +  
Sbjct: 82  GVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNY 141

Query: 169 MGSSAEHASQLWSSNYQPFCDVMEEYQKNMKSLAEKITHLILNFLKIPDDAEISNWLHSA 228
           + SS  + S+ W S      +++E+Y + ++ L E++T  +   L +    + +  + + 
Sbjct: 142 LPSSIRNPSK-WPSQPPKIRELIEKYGEEVRKLCERLTETLSESLGL----KPNKLMQAL 201

Query: 229 GAAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTI-LHQSSTCGLQIFRDGFGWLPV 288
           G       +L+ N YP+CP P   +GL+ H+D   +TI L      GLQ+ R G GW+ +
Sbjct: 202 GGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQV-RRGDGWVTI 261

Query: 289 TPVAGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFFGPPPDFCVTPFNNPP 348
             V  AL++N+GD   ILSNG + +V H+V+V+    R+S+A+F+ P  D  V P     
Sbjct: 262 KSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELV 321

Query: 349 ESPR---FRSVVVKEYVGLKAKNLECALSMIE 370
            + R   ++ +   EY  L  +   C  + ++
Sbjct: 322 TANRPALYKPIRFDEYRSLIRQKGPCGKNQVD 347

The following BLAST results are available for this feature:
BLAST of CmaCh05G006480 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O246482.6e-9147.06Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1[more]
Q391035.7e-8647.01Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ... [more]
Q9ZT846.3e-8545.71Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ... [more]
Q9SVS81.5e-7541.13Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ... [more]
Q9C9713.4e-7542.73Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ... [more]
BLAST of CmaCh05G006480 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G15550.14.0e-8747.01gibberellin 3-oxidase 1 [more]
AT1G80340.14.5e-8645.71gibberellin 3-oxidase 2 [more]
AT4G21690.11.1e-7641.13gibberellin 3-oxidase 3 [more]
AT1G80330.12.5e-7642.73gibberellin 3-oxidase 4 [more]
AT2G38240.15.3e-3929.522-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 184..204
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 251..272
score: 35.95
coord: 88..105
score: 39.9
coord: 287..313
score: 28.62
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 32..364
NoneNo IPR availablePANTHERPTHR47990:SF105GIBBERELLIN 2-BETA-DIOXYGENASEcoord: 32..364
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 37..359
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 37..355
e-value: 3.6E-75
score: 255.5
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 230..327
e-value: 5.6E-25
score: 87.8
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 74..168
e-value: 5.9E-17
score: 62.5
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 227..327
score: 13.073103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G006480.1CmaCh05G006480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009686 gibberellin biosynthetic process
biological_process GO:0009416 response to light stimulus
molecular_function GO:0016707 gibberellin 3-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding