CmaCh05G006250 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G006250
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAminoacyl-tRNA hydrolase
LocationCma_Chr05: 3139264 .. 3140556 (+)
RNA-Seq ExpressionCmaCh05G006250
SyntenyCmaCh05G006250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCTGTAATTTCAGCGTCTCGATTCACTTTCTCCATTTCCAAATCCACGAAGCTCTGTTCGTGTTCGAGGTCTAGGGTTTGGCCCTCTTCTTCACTCAGTCTCCGCTCTCGTAACCGAAATTCGAATCTATTTAGCTCCGCTTCTATGAGTGAATCCGCTGAAAGTAATTCCGCTCCTCCACCGGACGACATCGTGGAGAAGGCAGCCGACGTTTTGATTCAGTACGTGGTGCTCCGGAGAGATCTGATCGATTCGTGGCCACTTGGAAGCGTCGTAACTCAGGGATGCCATGCTTCAGTCTCCGCTATTTGGTTGAACAAAGACGATCCCCAAACTTCCGAGTACTGTAGTCCACACAATATCGATTCTATGCATAAGGTTGGTCGAACTTCTGATTCCATTTTTAAAATTTGATGTGTTGTGCTTCGAACTCGGACGGACAAAATGTAGTATCCATGTGTAGCCTCGAACCTTCTCTGTTGATAAACTGGAAATTTGCTCCGTTGGTGCGCTGTTAAGTCGTTCTAGATGAGACAGTCTGAGCAATTTGGAGCTTTCGTGTTATGGTTCTGGTCGCTGGTTGATTTATTTAATTCTGGAAAGAACATTTTGCGTCTGTCAAGCATTTTGGAAGAACAATTGTTTACTGGAAAACTATTTTTGTTTCGGTTCTTGTGTTTCTCCATTTAAACTATATTTTTGCTATTCTGAAGTTTGAGTCATCCCTGCTCGTATTGTTTGCTGAATGTTCTGATTTTTCCACCTAATATTCATACGGTTGTATCTCTCTAGGCTCTCCACCGTATTCGTAGAGTCGTAGGGAAAGAAGAGGGTTGTTTTTTTAGTTCTACAGCTTCCAAGGAATTTTTCACCTCCATTTCGCATTGGCCTGTGTAACCAGCCCCATAGTTAATGCTAACCTCATCATTTCAAGCTTGCAAAACGATTGAACTGCCAATTGGAAGTTTCCTTGCACATCAGATTCTTATCGCGTGCAACTTAATTAGTTGAAATTATAGTTGTTCTGCATTTCATTGATCCCTTAAGTCGTTCTGGACTTCTTGTTCCCTGAAGTCAGTACTGACTTTGTTTAACTTTATGTAGGTTACTCTTGAGGTGAAGGGAGAAACTCAGATCGTCAACTTGTCGGAGAAACTTAAAGAAAATTCAATCGCCCACAAACTATGGATAGAACAACCAGAGAACATTCCAACATGTCTTGCGACCAAACCCTACCCCAAATCTGTTGTATCAGCATTTTTCAAAAAACTGAAACTCTGCAAGTGA

mRNA sequence

ATGATTTCTGTAATTTCAGCGTCTCGATTCACTTTCTCCATTTCCAAATCCACGAAGCTCTGTTCGTGTTCGAGGTCTAGGGTTTGGCCCTCTTCTTCACTCAGTCTCCGCTCTCGTAACCGAAATTCGAATCTATTTAGCTCCGCTTCTATGAGTGAATCCGCTGAAAGTAATTCCGCTCCTCCACCGGACGACATCGTGGAGAAGGCAGCCGACGTTTTGATTCAGTACGTGGTGCTCCGGAGAGATCTGATCGATTCGTGGCCACTTGGAAGCGTCGTAACTCAGGGATGCCATGCTTCAGTCTCCGCTATTTGGTTGAACAAAGACGATCCCCAAACTTCCGAGTACTGTAGTCCACACAATATCGATTCTATGCATAAGGTTACTCTTGAGGTGAAGGGAGAAACTCAGATCGTCAACTTGTCGGAGAAACTTAAAGAAAATTCAATCGCCCACAAACTATGGATAGAACAACCAGAGAACATTCCAACATGTCTTGCGACCAAACCCTACCCCAAATCTGTTGTATCAGCATTTTTCAAAAAACTGAAACTCTGCAAGTGA

Coding sequence (CDS)

ATGATTTCTGTAATTTCAGCGTCTCGATTCACTTTCTCCATTTCCAAATCCACGAAGCTCTGTTCGTGTTCGAGGTCTAGGGTTTGGCCCTCTTCTTCACTCAGTCTCCGCTCTCGTAACCGAAATTCGAATCTATTTAGCTCCGCTTCTATGAGTGAATCCGCTGAAAGTAATTCCGCTCCTCCACCGGACGACATCGTGGAGAAGGCAGCCGACGTTTTGATTCAGTACGTGGTGCTCCGGAGAGATCTGATCGATTCGTGGCCACTTGGAAGCGTCGTAACTCAGGGATGCCATGCTTCAGTCTCCGCTATTTGGTTGAACAAAGACGATCCCCAAACTTCCGAGTACTGTAGTCCACACAATATCGATTCTATGCATAAGGTTACTCTTGAGGTGAAGGGAGAAACTCAGATCGTCAACTTGTCGGAGAAACTTAAAGAAAATTCAATCGCCCACAAACTATGGATAGAACAACCAGAGAACATTCCAACATGTCTTGCGACCAAACCCTACCCCAAATCTGTTGTATCAGCATTTTTCAAAAAACTGAAACTCTGCAAGTGA

Protein sequence

MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSAPPPDDIVEKAADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPQTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLATKPYPKSVVSAFFKKLKLCK
Homology
[Close]
BLAST of CmaCh05G006250 vs. ExPASy Swiss-Prot
Match: Q3SZ85 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 1.8e-27
Identity = 59/119 (49.58%), Postives = 82/119 (68.91%), Query Frame = 0 

Query: 73  VLIQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDPQTSEYCSPHNIDSMHKV 132
           +L+QY+VLR+DL     SWP G+VV Q CHA+ +A+ L++D P T+ Y     ++ M KV
Sbjct: 24  ILVQYLVLRKDLTQPPFSWPAGAVVAQACHAATAALHLHRDHPHTAAYL--RELERMRKV 83

Query: 133 TLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLATKPYPKSVVSAFFKKLKLCK 189
            LE   ET +  L+E L++ +I HKLW+EQPENIPTC+A +PYPK  VS + KK +L K
Sbjct: 84  VLEAPDETTLKLLAETLQQKNIDHKLWMEQPENIPTCIALRPYPKEEVSQYLKKFRLFK 140

[Close]
BLAST of CmaCh05G006250 vs. ExPASy Swiss-Prot
Match: D3Z4S3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.7e-25
Identity = 56/119 (47.06%), Postives = 79/119 (66.39%), Query Frame = 0 

Query: 73  VLIQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDPQTSEYCSPHNIDSMHKV 132
           +L+QY+VLR+DL     SWP G++V Q CHA+ +A+ L++D P T+ Y     +  M KV
Sbjct: 24  ILVQYLVLRKDLSQAPFSWPTGALVAQACHAATAALHLHRDHPHTAAYL--RELGRMRKV 83

Query: 133 TLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLATKPYPKSVVSAFFKKLKLCK 189
            LE   ET +  L+E L++ +I H LW+EQPENI TC+A +PYPK  VS + KK +L K
Sbjct: 84  VLEAADETTLKELAETLQQKNIDHTLWLEQPENIATCIALRPYPKEEVSQYLKKFRLFK 140

[Close]
BLAST of CmaCh05G006250 vs. ExPASy Swiss-Prot
Match: Q6GMV3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.4e-24
Identity = 55/119 (46.22%), Postives = 77/119 (64.71%), Query Frame = 0 

Query: 73  VLIQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDPQTSEYCSPHNIDSMHKV 132
           VL+QY+VLR+DL     SWP G++V Q CHA+ +A+  ++D P T+ Y     +  M KV
Sbjct: 24  VLVQYLVLRKDLSQAPFSWPAGALVAQACHAATAALHTHRDHPHTAAYL--QELGRMRKV 83

Query: 133 TLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLATKPYPKSVVSAFFKKLKLCK 189
            LE   ET +  L+E L++ +I H LW+EQPENI TC+A +PYPK  V  + KK +L K
Sbjct: 84  VLEAPDETTLKELAETLQQKNIDHMLWLEQPENIATCIALRPYPKEEVGQYLKKFRLFK 140

[Close]
BLAST of CmaCh05G006250 vs. TAIR 10
Match: AT5G10700.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 203.8 bits (517), Expect = 1.3e-52
Identity = 111/188 (59.04%), Postives = 132/188 (70.21%), Query Frame = 0 

Query: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSA 60
           M + + ++ F F I          R R + S   ++R  +      +S SMS+      A
Sbjct: 1   MATAVLSAAFRFPIVTRRLSAPAPRIRFYHS---TIRFAHFRRFTTASPSMSQQTGEIDA 60

Query: 61  PPPDDIVEKAADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPQTSEYCSP 120
                  EK  DV++QYVVLRRDLIDSWPLGSVVTQGCHASV+AIW  KDDP T +YC P
Sbjct: 61  VVDASSAEKPDDVVVQYVVLRRDLIDSWPLGSVVTQGCHASVAAIWSFKDDPVTLQYCDP 120

Query: 121 HNIDSMHKVTLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLATKPYPKSVVSAF 180
            +IDSMHKVTLEVKGETQ++NLSEKLK   I+HKLW+EQPENIPTC+ATKPYPKS VS+F
Sbjct: 121 QHIDSMHKVTLEVKGETQMMNLSEKLKLGGISHKLWMEQPENIPTCIATKPYPKSQVSSF 180

Query: 181 FKKLKLCK 189
           FKKLKLCK
Sbjct: 181 FKKLKLCK 185

The following BLAST results are available for this feature:
BLAST of CmaCh05G006250 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q3SZ851.8e-2749.58Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=... [more]
D3Z4S31.7e-2547.06Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 ... [more]
Q6GMV31.4e-2446.22Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 S... [more]
BLAST of CmaCh05G006250 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G10700.11.3e-5259.04Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023476Peptidyl-tRNA hydrolase II domain superfamilyGENE3D3.40.1490.10Bit1coord: 74..187
e-value: 2.3E-18
score: 68.4
IPR023476Peptidyl-tRNA hydrolase II domain superfamilySUPERFAMILY102462Peptidyl-tRNA hydrolase IIcoord: 75..187
IPR002833Peptidyl-tRNA hydrolase, PTH2PFAMPF01981PTH2coord: 76..186
e-value: 2.9E-24
score: 85.7
IPR042237Putative peptidyl-tRNA hydrolase PTRHD1PANTHERPTHR46194PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATEDcoord: 40..188
NoneNo IPR availableCDDcd02429PTH2_likecoord: 74..187
e-value: 4.00186E-65
score: 194.16

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G006250.1CmaCh05G006250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004045 aminoacyl-tRNA hydrolase activity