CmaCh05G005540 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh05G005540
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	mitochondrial uncoupling protein 5-like
Location	Cma_Chr05: 2712676 .. 2713635 (+)
RNA-Seq Expression	CmaCh05G005540
Synteny	CmaCh05G005540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTGAAGGGATTCGCCGAAGGAGGAATCGCCTCCATTGTTGCTGGATGCTCCACTCACCCTCTTGATCTCATTAAAGTTCGGATGCAATTGGCCGGCGAGAAACCCACGCTTCCAAGTCTCCGCCCCGCCTTAGCTTTCAATGGGTCTTCTTCCGTTGTCGCGCCGGAATCTTTCCATATTCCCCCGCCTCAACCACCGCGTGCGGGGCCCATATCGGTTGGAGTACGGATTGTCCAGTCCGAAGGTGTTGCTGCTCTGTTTTCTGGCGTCTCCGCCACCGTTCTCCGTCAGACTCTTTACTCCACCACTCGGATGGGTCTCTACGACGTTCTTAAAAGTAAATGGTCCGACCCGAATTCTGGCAACATGCCTCTGACCCGGAAAATCACGGCGGGGCTTATTGCCGGTGGAATTGGCGCGGCGGTGGGGAACCCGGCGGATGTCGCAATGGTGAGAATGCAGGCCGATGGCAGGCTTCCGTTGAGTCAGCGGCGGAATTACGCCGGCGTGGTGGACGCCATCACAAGAATGTCAAAGAAGGAAGGGATTAGTAGCCTGTGGCGCGGGTCGTCGCTTACGGTGAACCGCGCTATGATCGTGACGGCGGCGCAGTTGGCGTCGTACGATGAAATCAAAGAGACGATATTGGAAAAGGGTGTGATGAAGGACGGATTGGGGACCCACGTGACGGCGAGTTTCGCGGCGGGATTTGTGGCGGCGGTGGCGTCAAATCCGGTGGATGTGATAAAGACGAGAGTTATGAATATGAAAGTGGAGAGCGGGGCGGCTCCGCCGTACAAGGGGGCGTTGGACTGCGCGATGAAGACGGTGAAGGCGGAAGGGGCAATGGCGCTTTACAAAGGATTTATTCCGACGATTTCACGGCAGGGACCTTTCACTGTAGTCTTGTTTGTCACACTCGAACAAGTTAGGAAGATTTTCAACCAGTTTTGA

mRNA sequence

ATGGGTTTGAAGGGATTCGCCGAAGGAGGAATCGCCTCCATTGTTGCTGGATGCTCCACTCACCCTCTTGATCTCATTAAAGTTCGGATGCAATTGGCCGGCGAGAAACCCACGCTTCCAAGTCTCCGCCCCGCCTTAGCTTTCAATGGGTCTTCTTCCGTTGTCGCGCCGGAATCTTTCCATATTCCCCCGCCTCAACCACCGCGTGCGGGGCCCATATCGGTTGGAGTACGGATTGTCCAGTCCGAAGGTGTTGCTGCTCTGTTTTCTGGCGTCTCCGCCACCGTTCTCCGTCAGACTCTTTACTCCACCACTCGGATGGGTCTCTACGACGTTCTTAAAAGTAAATGGTCCGACCCGAATTCTGGCAACATGCCTCTGACCCGGAAAATCACGGCGGGGCTTATTGCCGGTGGAATTGGCGCGGCGGTGGGGAACCCGGCGGATGTCGCAATGGTGAGAATGCAGGCCGATGGCAGGCTTCCGTTGAGTCAGCGGCGGAATTACGCCGGCGTGGTGGACGCCATCACAAGAATGTCAAAGAAGGAAGGGATTAGTAGCCTGTGGCGCGGGTCGTCGCTTACGGTGAACCGCGCTATGATCGTGACGGCGGCGCAGTTGGCGTCGTACGATGAAATCAAAGAGACGATATTGGAAAAGGGTGTGATGAAGGACGGATTGGGGACCCACGTGACGGCGAGTTTCGCGGCGGGATTTGTGGCGGCGGTGGCGTCAAATCCGGTGGATGTGATAAAGACGAGAGTTATGAATATGAAAGTGGAGAGCGGGGCGGCTCCGCCGTACAAGGGGGCGTTGGACTGCGCGATGAAGACGGTGAAGGCGGAAGGGGCAATGGCGCTTTACAAAGGATTTATTCCGACGATTTCACGGCAGGGACCTTTCACTGTAGTCTTGTTTGTCACACTCGAACAAGTTAGGAAGATTTTCAACCAGTTTTGA

Coding sequence (CDS)

ATGGGTTTGAAGGGATTCGCCGAAGGAGGAATCGCCTCCATTGTTGCTGGATGCTCCACTCACCCTCTTGATCTCATTAAAGTTCGGATGCAATTGGCCGGCGAGAAACCCACGCTTCCAAGTCTCCGCCCCGCCTTAGCTTTCAATGGGTCTTCTTCCGTTGTCGCGCCGGAATCTTTCCATATTCCCCCGCCTCAACCACCGCGTGCGGGGCCCATATCGGTTGGAGTACGGATTGTCCAGTCCGAAGGTGTTGCTGCTCTGTTTTCTGGCGTCTCCGCCACCGTTCTCCGTCAGACTCTTTACTCCACCACTCGGATGGGTCTCTACGACGTTCTTAAAAGTAAATGGTCCGACCCGAATTCTGGCAACATGCCTCTGACCCGGAAAATCACGGCGGGGCTTATTGCCGGTGGAATTGGCGCGGCGGTGGGGAACCCGGCGGATGTCGCAATGGTGAGAATGCAGGCCGATGGCAGGCTTCCGTTGAGTCAGCGGCGGAATTACGCCGGCGTGGTGGACGCCATCACAAGAATGTCAAAGAAGGAAGGGATTAGTAGCCTGTGGCGCGGGTCGTCGCTTACGGTGAACCGCGCTATGATCGTGACGGCGGCGCAGTTGGCGTCGTACGATGAAATCAAAGAGACGATATTGGAAAAGGGTGTGATGAAGGACGGATTGGGGACCCACGTGACGGCGAGTTTCGCGGCGGGATTTGTGGCGGCGGTGGCGTCAAATCCGGTGGATGTGATAAAGACGAGAGTTATGAATATGAAAGTGGAGAGCGGGGCGGCTCCGCCGTACAAGGGGGCGTTGGACTGCGCGATGAAGACGGTGAAGGCGGAAGGGGCAATGGCGCTTTACAAAGGATTTATTCCGACGATTTCACGGCAGGGACCTTTCACTGTAGTCTTGTTTGTCACACTCGAACAAGTTAGGAAGATTTTCAACCAGTTTTGA

Protein sequence

MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKPTLPSLRPALAFNGSSSVVAPESFHIPPPQPPRAGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKSKWSDPNSGNMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPLSQRRNYAGVVDAITRMSKKEGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEKGVMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTVKAEGAMALYKGFIPTISRQGPFTVVLFVTLEQVRKIFNQF

Homology

[Close]

BLAST of CmaCh05G005540 vs. ExPASy Swiss-Prot
Match: Q9SJY5 (Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 4.2e-130
Identity = 239/320 (74.69%), Postives = 275/320 (85.94%), Query Frame = 0


Query: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEK-PTLPSLRPALAFNGSSSVVAPES 60
           MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQL GE  P   +LRPALAF  S++V AP  
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAP-- 60

Query: 61  FHIPPPQPPRAGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKSKWSD 120
                  P R G I VG R+++ EG+ ALFSGVSATVLRQTLYSTTRMGLYD++K +W+D
Sbjct: 61  -------PLRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTD 120

Query: 121 PNSGNMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPLSQRRNYAGVVDAITRM 180
           P +  MPL +KI AG IAG IGAAVGNPADVAMVRMQADGRLPL+ RRNY  V+DAIT+M
Sbjct: 121 PETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQM 180

Query: 181 SKKEGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEKGVMKDGLGTHVTASFAAGF 240
            + EG++SLWRGSSLT+NRAM+VT++QLASYD +KETILEKG++KDGLGTHV+ASFAAGF
Sbjct: 181 IRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGF 240

Query: 241 VAAVASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTVKAEGAMALYKGFIPTISRQG 300
           VA+VASNPVDVIKTRVMNMKV +G APPYKGA+DCA+KTVKAEG M+LYKGFIPT+SRQ 
Sbjct: 241 VASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQA 300

Query: 301 PFTVVLFVTLEQVRKIFNQF 320
           PFTVVLFVTLEQV+K+F  +
Sbjct: 301 PFTVVLFVTLEQVKKLFKDY 311

[Close]

BLAST of CmaCh05G005540 vs. ExPASy Swiss-Prot
Match: Q9SB52 (Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 5.7e-127
Identity = 243/322 (75.47%), Postives = 271/322 (84.16%), Query Frame = 0


Query: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKP---TLPSLRPALAFNGSSSVVAP 60
           MG+K F EGGIAS++AGCSTHPLDLIKVR+QL GE P   T+  LRPALAF  SS    P
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNSS----P 60

Query: 61  ESFHIPPPQPPRAGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKSKW 120
            +F       P+ GPIS+G+ IV+SEG AALFSGVSAT+LRQTLYSTTRMGLY+VLK+KW
Sbjct: 61  AAFLETTSSVPKVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKW 120

Query: 121 SDPNSGNMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPLSQRRNYAGVVDAIT 180
           +DP SG + L+RKI AGL+AGGIGAAVGNPADVAMVRMQADGRLPL+QRRNYAGV DAI 
Sbjct: 121 TDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDAIR 180

Query: 181 RMSKKEGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEKGVMKDGLGTHVTASFAA 240
            M K EG++SLWRGS+LT+NRAMIVTAAQLASYD+ KE ILE GVM DGLGTHV ASFAA
Sbjct: 181 SMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAA 240

Query: 241 GFVAAVASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTVKAEGAMALYKGFIPTISR 300
           GFVA+VASNPVDVIKTRVMNMKV  GA   Y GA DCA+KTVKAEGAMALYKGF+PT+ R
Sbjct: 241 GFVASVASNPVDVIKTRVMNMKV--GA---YDGAWDCAVKTVKAEGAMALYKGFVPTVCR 300

Query: 301 QGPFTVVLFVTLEQVRKIFNQF 320
           QGPFTVVLFVTLEQVRK+   F
Sbjct: 301 QGPFTVVLFVTLEQVRKLLRDF 313

[Close]

BLAST of CmaCh05G005540 vs. ExPASy Swiss-Prot
Match: Q9FY68 (Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.2e-97
Identity = 198/334 (59.28%), Postives = 235/334 (70.36%), Query Frame = 0


Query: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGE---------KPTL--------PSLR 60
           MG K F EGGIA+I+AG  THPLDLIKVRMQL GE          P L           R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFNGSSSVVAPESFHIPPPQPPRAG---PISVGVRIVQSEGVAALFSGVSATVLRQT 120
           P  A +     ++    HI  P         P +VG  IV++EG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 LYSTTRMGLYDVLKSKWSDPNSGNMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGR 180
           LYS TRMG+YD LK +W+D  +GN PL  KITAGLIAG +G+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPLSQRRNYAGVVDAITRMSKKEGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEK 240
           LPL++RRNY  VVDAI R++++EG+SSLWRGS LTVNRAMIVTA+QLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 G-VMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKEI-----YGGPLDCAVKMV 300

Query: 301 KAEGAMALYKGFIPTISRQGPFTVVLFVTLEQVR 314
             EG MALYKG +PT +RQGPFT++LF+TLEQVR
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVR 329

[Close]

BLAST of CmaCh05G005540 vs. ExPASy Swiss-Prot
Match: Q54PY7 (Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium discoideum OX=44689 GN=ucpC PE=3 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.6e-65
Identity = 138/316 (43.67%), Postives = 192/316 (60.76%), Query Frame = 0


Query: 3   LKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKPTLPSLRPALAFNGSSSVVAPESFHI 62
           LK F  GG+A +++   THP+D +KVRMQL GE   +                       
Sbjct: 25  LKQFVIGGLAGMLSSAFTHPIDSLKVRMQLQGEGTGV----------------------- 84

Query: 63  PPPQPPRAGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKSKWSDPNS 122
                P+ G + + V I Q+EG   L+ G+SA++LRQ  Y+TTR GLYD++K   +  + 
Sbjct: 85  ----GPKRGALKMLVHINQTEGFFTLYKGLSASLLRQATYTTTRFGLYDLIKDIVAKDDK 144

Query: 123 GNMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPLSQRRNYAGVVDAITRMSKK 182
             +P T+KI  G+++G  GA VG PAD+ MVRMQADG+LP + RRNY  V D I R+SK+
Sbjct: 145 -PLPFTQKIMVGMLSGAGGAIVGTPADLTMVRMQADGKLPFNLRRNYKNVFDGIFRISKE 204

Query: 183 EGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEKGVMKDGLGTHVTASFAAGFVAA 242
           EGI SLW+G S  + RAM +TA Q++SYD+ K+ +L  G   D + TH+ AS  A FVAA
Sbjct: 205 EGIISLWKGCSPNLIRAMFMTAGQVSSYDQTKQLMLASGYFHDDIKTHLIASTTAAFVAA 264

Query: 243 VASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTVKAEGAMALYKGFIPTISRQGPFT 302
           VA++P+DVIKTR+MN          YKG  DC  KT++AEG  A YKGF P   R GP T
Sbjct: 265 VATSPLDVIKTRIMNSPKTVTGELQYKGTFDCLSKTLRAEGFKAFYKGFNPYFMRLGPQT 312

Query: 303 VVLFVTLEQVRKIFNQ 319
           ++ F+ +EQ+  ++ +
Sbjct: 325 ILTFIFVEQLNILWKK 312

[Close]

BLAST of CmaCh05G005540 vs. ExPASy Swiss-Prot
Match: P90992 (Mitochondrial 2-oxoglutarate/malate carrier protein OS=Caenorhabditis elegans OX=6239 GN=misc-1 PE=1 SV=2)

HSP 1 Score: 230.3 bits (586), Expect = 3.0e-59
Identity = 133/316 (42.09%), Postives = 187/316 (59.18%), Query Frame = 0


Query: 6   FAEGGIASIVAGCSTHPLDLIKVRMQLAGE--KPTLPSLRPALAFNGSSSVVAPESFHIP 65
           FA GG A + A     PLDL+K RMQL+G   K    S   AL                 
Sbjct: 13  FAFGGTAGMGATLVVQPLDLVKNRMQLSGTTGKKEYRSSMHAL----------------- 72

Query: 66  PPQPPRAGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKSKWSDPNSG 125
                          I+++EGV A+++G+SA +LRQ  Y+TTR+G Y  L  ++++ +  
Sbjct: 73  -------------TSIMKNEGVFAVYNGLSAGLLRQATYTTTRLGTYAFLLERFTEKDK- 132

Query: 126 NMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPLSQRRNYAGVVDAITRMSKKE 185
            +    K   G+ AGGIG+ VG PA++A++RM  DGRLP+ QRRNY GVV+A+TR++K+E
Sbjct: 133 PLSFGMKAVLGMTAGGIGSFVGTPAEIALIRMTGDGRLPVEQRRNYTGVVNALTRITKEE 192

Query: 186 GISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEKGVMKDGLGTHVTASFAAGFVAAV 245
           G+ +LWRG + TV RAM+V AAQLA+Y + K+ +L  G ++DG+  H  AS  +G    +
Sbjct: 193 GVLTLWRGCTPTVLRAMVVNAAQLATYSQAKQALLASGKVQDGIFCHFLASMISGLATTI 252

Query: 246 ASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTVKAEGAMALYKGFIPTISRQGPFTV 305
           AS PVD+ KTR+ +MKV  G  P YK A D   K +K EG  AL+KGF P   R GP TV
Sbjct: 253 ASMPVDIAKTRIQSMKVIDG-KPEYKNAFDVWGKVIKNEGIFALWKGFTPYYMRLGPHTV 296

Query: 306 VLFVTLEQVRKIFNQF 320
           + F+ LEQ+   + Q+
Sbjct: 313 LTFIILEQMNAAYFQY 296

[Close]

BLAST of CmaCh05G005540 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 465.7 bits (1197), Expect = 3.0e-131
Identity = 239/320 (74.69%), Postives = 275/320 (85.94%), Query Frame = 0


Query: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEK-PTLPSLRPALAFNGSSSVVAPES 60
           MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQL GE  P   +LRPALAF  S++V AP  
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAP-- 60

Query: 61  FHIPPPQPPRAGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKSKWSD 120
                  P R G I VG R+++ EG+ ALFSGVSATVLRQTLYSTTRMGLYD++K +W+D
Sbjct: 61  -------PLRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTD 120

Query: 121 PNSGNMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPLSQRRNYAGVVDAITRM 180
           P +  MPL +KI AG IAG IGAAVGNPADVAMVRMQADGRLPL+ RRNY  V+DAIT+M
Sbjct: 121 PETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQM 180

Query: 181 SKKEGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEKGVMKDGLGTHVTASFAAGF 240
            + EG++SLWRGSSLT+NRAM+VT++QLASYD +KETILEKG++KDGLGTHV+ASFAAGF
Sbjct: 181 IRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGF 240

Query: 241 VAAVASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTVKAEGAMALYKGFIPTISRQG 300
           VA+VASNPVDVIKTRVMNMKV +G APPYKGA+DCA+KTVKAEG M+LYKGFIPT+SRQ 
Sbjct: 241 VASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQA 300

Query: 301 PFTVVLFVTLEQVRKIFNQF 320
           PFTVVLFVTLEQV+K+F  +
Sbjct: 301 PFTVVLFVTLEQVKKLFKDY 311

[Close]

BLAST of CmaCh05G005540 vs. TAIR 10
Match: AT4G24570.1 (dicarboxylate carrier 2 )

HSP 1 Score: 455.3 bits (1170), Expect = 4.1e-128
Identity = 243/322 (75.47%), Postives = 271/322 (84.16%), Query Frame = 0


Query: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKP---TLPSLRPALAFNGSSSVVAP 60
           MG+K F EGGIAS++AGCSTHPLDLIKVR+QL GE P   T+  LRPALAF  SS    P
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNSS----P 60

Query: 61  ESFHIPPPQPPRAGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKSKW 120
            +F       P+ GPIS+G+ IV+SEG AALFSGVSAT+LRQTLYSTTRMGLY+VLK+KW
Sbjct: 61  AAFLETTSSVPKVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKW 120

Query: 121 SDPNSGNMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPLSQRRNYAGVVDAIT 180
           +DP SG + L+RKI AGL+AGGIGAAVGNPADVAMVRMQADGRLPL+QRRNYAGV DAI 
Sbjct: 121 TDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDAIR 180

Query: 181 RMSKKEGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEKGVMKDGLGTHVTASFAA 240
            M K EG++SLWRGS+LT+NRAMIVTAAQLASYD+ KE ILE GVM DGLGTHV ASFAA
Sbjct: 181 SMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAA 240

Query: 241 GFVAAVASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTVKAEGAMALYKGFIPTISR 300
           GFVA+VASNPVDVIKTRVMNMKV  GA   Y GA DCA+KTVKAEGAMALYKGF+PT+ R
Sbjct: 241 GFVASVASNPVDVIKTRVMNMKV--GA---YDGAWDCAVKTVKAEGAMALYKGFVPTVCR 300

Query: 301 QGPFTVVLFVTLEQVRKIFNQF 320
           QGPFTVVLFVTLEQVRK+   F
Sbjct: 301 QGPFTVVLFVTLEQVRKLLRDF 313

[Close]

BLAST of CmaCh05G005540 vs. TAIR 10
Match: AT5G09470.1 (dicarboxylate carrier 3 )

HSP 1 Score: 357.8 bits (917), Expect = 8.8e-99
Identity = 198/334 (59.28%), Postives = 235/334 (70.36%), Query Frame = 0


Query: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGE---------KPTL--------PSLR 60
           MG K F EGGIA+I+AG  THPLDLIKVRMQL GE          P L           R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFNGSSSVVAPESFHIPPPQPPRAG---PISVGVRIVQSEGVAALFSGVSATVLRQT 120
           P  A +     ++    HI  P         P +VG  IV++EG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 LYSTTRMGLYDVLKSKWSDPNSGNMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGR 180
           LYS TRMG+YD LK +W+D  +GN PL  KITAGLIAG +G+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPLSQRRNYAGVVDAITRMSKKEGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEK 240
           LPL++RRNY  VVDAI R++++EG+SSLWRGS LTVNRAMIVTA+QLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 G-VMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKEI-----YGGPLDCAVKMV 300

Query: 301 KAEGAMALYKGFIPTISRQGPFTVVLFVTLEQVR 314
             EG MALYKG +PT +RQGPFT++LF+TLEQVR
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVR 329

[Close]

BLAST of CmaCh05G005540 vs. TAIR 10
Match: AT5G58970.1 (uncoupling protein 2 )

HSP 1 Score: 205.7 bits (522), Expect = 5.6e-53
Identity = 124/316 (39.24%), Postives = 175/316 (55.38%), Query Frame = 0


Query: 6   FAEGGIASIVAGC----STHPLDLIKVRMQLAGEKPTLPSLRPALAFNGSSSVVAPESFH 65
           F E  I S  A C     T PLD  KVR+QL  + PT           G    +      
Sbjct: 12  FLETFICSAFAACFAELCTIPLDTAKVRLQLQRKIPT-----------GDGENL------ 71

Query: 66  IPPPQPPRAGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKSKWSDPN 125
                P   G I     I + EG++ L+ GV A + RQ +Y   R+GLY+ +K+     +
Sbjct: 72  -----PKYRGSIGTLATIAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSD 131

Query: 126 -SGNMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPLSQRRNYAGVVDAITRMS 185
             G++PL +KI A L+ G I   V NP D+  VR+Q++G+LP    R YAG VDA   + 
Sbjct: 132 FIGDIPLYQKILAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDAYFTIV 191

Query: 186 KKEGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEKGVMKDGLGTHVTASFAAGFV 245
           K EG+S+LW G    + R  IV AA+LASYD+IKETI++    +D + TH+ A  AAGF 
Sbjct: 192 KLEGVSALWTGLGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFF 251

Query: 246 AAVASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTVKAEGAMALYKGFIPTISRQGP 305
           A    +P+DV+K+R+M           Y+  +DC +KT+K EG MA YKGF+P  +R G 
Sbjct: 252 AVCIGSPIDVVKSRMMGDST-------YRNTVDCFIKTMKTEGIMAFYKGFLPNFTRLGT 298

Query: 306 FTVVLFVTLEQVRKIF 317
           +  ++F+TLEQV+K+F
Sbjct: 312 WNAIMFLTLEQVKKVF 298

[Close]

BLAST of CmaCh05G005540 vs. TAIR 10
Match: AT5G19760.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.1e-52
Identity = 122/318 (38.36%), Postives = 175/318 (55.03%), Query Frame = 0


Query: 3   LKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKPTLPSLRPALAFNGSSSVVAPESFHI 62
           +K F  GG + ++A C   P+D+IKVR+QL                 GS++         
Sbjct: 15  VKPFVNGGASGMLATCVIQPIDMIKVRIQLG---------------QGSAA--------- 74

Query: 63  PPPQPPRAGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKSKWSDPNS 122
                      S+   ++++EGV A + G+SA +LRQ  Y+T R+G + +L +K  + N 
Sbjct: 75  -----------SITTNMLKNEGVGAFYKGLSAGLLRQATYTTARLGSFKLLTAKAIESND 134

Query: 123 GN-MPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPLSQRRNYAGVVDAITRMSK 182
           G  +PL +K   GL AG IGA VG+PAD+A++RMQAD  LPL+QRRNY     A+TR+S 
Sbjct: 135 GKPLPLYQKALCGLTAGAIGACVGSPADLALIRMQADNTLPLAQRRNYTNAFHALTRISA 194

Query: 183 KEGISSLWRGSSLTVNRAMIVTAAQLASYDEIKETILEKGVMKDGLG-----THVTASFA 242
            EG+ +LW+G   TV RAM +    LASYD+  E       M+D LG     T V AS  
Sbjct: 195 DEGVLALWKGCGPTVVRAMALNMGMLASYDQSAE------YMRDNLGFGEMSTVVGASAV 254

Query: 243 AGFVAAVASNPVDVIKTRVMNMKVESGAAPPYKGALDCAMKTVKAEGAMALYKGFIPTIS 302
           +GF AA  S P D +KT++  M+ ++    PY G+LDCAMKT+K  G +  Y GF     
Sbjct: 255 SGFCAAACSLPFDFVKTQIQKMQPDAQGKYPYTGSLDCAMKTLKEGGPLKFYSGFPVYCV 291

Query: 303 RQGPFTVVLFVTLEQVRK 315
           R  P  ++ ++ L Q+ K
Sbjct: 315 RIAPHVMMTWIFLNQITK 291

The following BLAST results are available for this feature:

BLAST of CmaCh05G005540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SJY5	4.2e-130	74.69	Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2...	[more]
Q9SB52	5.7e-127	75.47	Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2...	[more]
Q9FY68	1.2e-97	59.28	Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2...	[more]
Q54PY7	3.6e-65	43.67	Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium di...	[more]
P90992	3.0e-59	42.09	Mitochondrial 2-oxoglutarate/malate carrier protein OS=Caenorhabditis elegans OX...	[more]

BLAST of CmaCh05G005540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22500.1	3.0e-131	74.69	uncoupling protein 5	[more]
AT4G24570.1	4.1e-128	75.47	dicarboxylate carrier 2	[more]
AT5G09470.1	8.8e-99	59.28	dicarboxylate carrier 3	[more]
AT5G58970.1	5.6e-53	39.24	uncoupling protein 2	[more]
AT5G19760.1	2.1e-52	38.36	Mitochondrial substrate carrier family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002067	Mitochondrial carrier protein	PRINTS	PR00926	MITOCARRIER	coord: 5..18 score: 30.68 coord: 18..32 score: 24.26 coord: 140..158 score: 25.19 coord: 234..256 score: 28.65
IPR018108	Mitochondrial substrate/solute carrier	PFAM	PF00153	Mito_carr	coord: 125..219 e-value: 1.2E-18 score: 66.8 coord: 5..117 e-value: 2.6E-16 score: 59.3 coord: 228..318 e-value: 2.0E-19 score: 69.3
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 4..116 score: 17.557344
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 125..216 score: 26.288755
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 225..315 score: 26.975256
IPR023395	Mitochondrial carrier domain superfamily	GENE3D	1.50.40.10	Mitochondrial carrier domain	coord: 4..318 e-value: 7.5E-84 score: 283.0
IPR023395	Mitochondrial carrier domain superfamily	SUPERFAMILY	103506	Mitochondrial carrier	coord: 4..312
None	No IPR available	PANTHER	PTHR45618:SF45	MITOCHONDRIAL UNCOUPLING PROTEIN 5	coord: 1..319
None	No IPR available	PANTHER	PTHR45618	MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED	coord: 1..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh05G005540.1	CmaCh05G005540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071423	malate transmembrane transport
biological_process	GO:0006839	mitochondrial transport
biological_process	GO:1902356	oxaloacetate(2-) transmembrane transport
biological_process	GO:0035435	phosphate ion transmembrane transport
biological_process	GO:0071422	succinate transmembrane transport
biological_process	GO:1902358	sulfate transmembrane transport
biological_process	GO:0015709	thiosulfate transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0031966	mitochondrial membrane
molecular_function	GO:0015297	antiporter activity
molecular_function	GO:0015140	malate transmembrane transporter activity
molecular_function	GO:0015131	oxaloacetate transmembrane transporter activity
molecular_function	GO:0015141	succinate transmembrane transporter activity
molecular_function	GO:0015116	sulfate transmembrane transporter activity
molecular_function	GO:0015117	thiosulfate transmembrane transporter activity