CmaCh05G005230 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh05G005230 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 7.3e-17
Identity = 57/204 (27.94%), Postives = 90/204 (44.12%), Query Frame = 0

Query: 12  RILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSE------------VE 71
           ++LCLHG+  SG +  K +      +    + HF   P  +  +++            + 
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALG 65

Query: 72  GLHDPPYYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQ 131
           G  +   + WF+  +    + + +E L+ I  +M E GPFDGL+GFSQGA + A+L    
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQML 125

Query: 132 AKGV---ALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMP--S 191
             G       + P  KFV+ + G + E P  D    Y   +  PSLH     D  +P   
Sbjct: 126 QPGQPPNPYVQHPPFKFVVFVGGFRAEKPEFD--HFYNPKLTTPSLHIAGTSDTLVPLAR 185

Query: 192 GLQLLESFVEPFVINHLKGHAVPR 199
             QL+E      V+ H   H VP+
Sbjct: 186 SKQLVERCENAHVLLHPGQHIVPQ 207

BLAST of CmaCh05G005230 vs. ExPASy Swiss-Prot
Match: Q9VDL1 (Esterase CG5412 OS=Drosophila melanogaster OX=7227 GN=CG5412 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-16
Identity = 67/225 (29.78%), Postives = 101/225 (44.89%), Query Frame = 0

Query: 4   KSRIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRG-KSEVEGLH 63
           K  I  K R+LCLHG+R +G   K  +  +        +F F+ AP  ++  +S  E + 
Sbjct: 24  KLEITEKVRVLCLHGYRQNGEAFKNKLGSFRKFANKYAEFVFITAPHVAKALESAAEPV- 83

Query: 64  DPPYYEWFQFHEDTADF--TN-------VEECLQFIENHMLEHGPFDGLLGFSQGAALAA 123
            P    W+  ++D   F  TN        +E L+ +E      GPF GLLGFSQGA    
Sbjct: 84  -PEQRSWWA-NKDDGSFKGTNKGGPAFGFQESLRCVEEAWRTQGPFQGLLGFSQGACFVG 143

Query: 124 LLPGFQAKGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMP 183
           L+ G   K +   R    +F ++ +G    S SL    AY  +I+ P+LH   + D  +P
Sbjct: 144 LICGLAKKKLTSIRP---EFAVLASG--FLSGSLVHMSAYEEAISIPTLHIYGQTDEIIP 203

Query: 184 SGL--QLLESFVEPFVINHLKGHAVPRLDQKSLKIMENFIDRISK 217
             +   L   F    V+ H  GH  P   Q+    +  F DR+ +
Sbjct: 204 KEMSESLAARFKNAEVLEHSGGHYFPATAQQKQTFINFFQDRLQE 240

BLAST of CmaCh05G005230 vs. ExPASy Swiss-Prot
Match: Q7QBJ0 (Esterase AGAP003155 OS=Anopheles gambiae OX=7165 GN=AGAP003155 PE=3 SV=3)

HSP 1 Score: 85.9 bits (211), Expect = 6.2e-16
Identity = 67/216 (31.02%), Postives = 101/216 (46.76%), Query Frame = 0

Query: 8   QRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVEGLHDPPYY 67
           + K ++L LHG+R +    K  +  +   +    +F F+ AP P+     V G  DP   
Sbjct: 9   EEKLKVLALHGYRQNADSFKSKLGSFRKMLNKYVEFVFVSAPHPAAPLEAVGGEPDPNQR 68

Query: 68  EWFQFHEDTADF--TN-------VEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGF 127
            W+ F++D   F  TN        +E L+ +E      G   GLLGFSQGA    LL   
Sbjct: 69  SWW-FNKDDRTFKGTNQGGPAYGFDESLRLVERTWQAEG-CHGLLGFSQGACFVGLLCDL 128

Query: 128 QAKGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMPSGLQ- 187
            A+G+      K +F ++ +G +  S SL     Y + +  PSLH   E D  +   +  
Sbjct: 129 SARGMT---TMKPQFAVVASGFR--SGSLVHLNYYENKVQIPSLHIFGETDEIITKDMSE 188

Query: 188 -LLESFVEPFVINHLKGHAVPRLDQKSLKIMENFID 213
            L E+F++P V+ H  GH  P   Q SLK  E ++D
Sbjct: 189 ALAETFLDPEVVTHPGGHYFPA--QASLK--ETYVD 213

BLAST of CmaCh05G005230 vs. ExPASy Swiss-Prot
Match: Q29BR3 (Esterase GA18864 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA18864 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.0e-15
Identity = 65/224 (29.02%), Postives = 96/224 (42.86%), Query Frame = 0

Query: 4   KSRIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAP-FPSRGKSEVEGLH 63
           K  I  K R+LCLHG+R  G   K  +  +        +F F+ AP   +  +S  E + 
Sbjct: 24  KLEITEKVRVLCLHGYRQDGDAFKNKLGSFRKFTSKYAEFVFISAPHIAAPLESAAEPV- 83

Query: 64  DPPYYEWFQFHED-TADFTN-------VEECLQFIENHMLEHGPFDGLLGFSQGAALAAL 123
            P    W+   +D T   TN        ++ L+ +E      GPF GLLGFSQGA    L
Sbjct: 84  -PEQRSWWANKDDGTFKGTNKGGPAFGFQDSLRLVEEAWKTQGPFQGLLGFSQGACFVGL 143

Query: 124 LPGFQAKGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMPS 183
           + G   K +   R    +F ++ +G    S SL    AY   ++ P+LH     D  +P 
Sbjct: 144 ICGLAKKKLTSIRP---EFAVLSSG--FVSGSLVHMSAYEEPVSIPTLHIYGSSDEIIPK 203

Query: 184 GLQ--LLESFVEPFVINHLKGHAVPRLDQKSLKIMENFIDRISK 217
            +   L   F    V+ H  GH  P   Q+    +  F DR+ +
Sbjct: 204 DMSALLASHFKNVEVLEHGGGHYFPATAQQKQTYINFFQDRLQE 240

BLAST of CmaCh05G005230 vs. ExPASy Swiss-Prot
Match: Q3SZ07 (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.7e-13
Identity = 64/223 (28.70%), Postives = 95/223 (42.60%), Query Frame = 0

Query: 12  RILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVEGL---------H 71
           RILCL GFR S    ++       ++  + +   +  P P    +  EG           
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 72  DPPYYEWFQFH--------EDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAAL 131
           + P   WF           E+      +EE L+ +   + + GPFDG+LGFSQGAALAAL
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 132 LPGFQAKGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMPS 191
           +      G    R P  +FVI+++G       L  +      ++ PSLH   + D  +PS
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGL-MEPIMQGPLSLPSLHVFGDTDGVIPS 187

Query: 192 --GLQLLESFVEPFVINHLKGHAVPRL---DQKSLKIMENFID 213
              +QL   F     + H  GH +P      Q  LK ++ F D
Sbjct: 188 QESMQLCSRFDGAVTLTHSGGHFIPAAAPQRQAYLKFLDQFAD 227

BLAST of CmaCh05G005230 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 272.3 bits (695), Expect = 3.3e-73
Identity = 129/218 (59.17%), Postives = 167/218 (76.61%), Query Frame = 0

Query: 1   MGSKSR-IQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVE 60
           MGS+ R I RKPR LCLHGFRTSG I+K  + KWP SV+++ D  F+DAPFP +GKS+VE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GLHDPPYYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQ 120
           G+ DPPYYEWFQF+++  ++TN E+CL+++E+ M++ GPFDGL+GFSQGA L+  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 AKGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMPSGLQLL 180
           AKG+A  +VPKIKFVIII G KL+S  L A+ AY+SS+   SLHFL E DF  P G QL+
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKL-AENAYSSSLETLSLHFLGETDFLKPYGTQLI 180

Query: 181 ESFVEPFVINHLKGHAVPRLDQKSLKIMENFIDRISKL 218
           ES+  P V++H KGH VPRLD+KSL+ +  FID +  L
Sbjct: 181 ESYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHL 217

BLAST of CmaCh05G005230 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 238.8 bits (608), Expect = 4.1e-63
Identity = 112/215 (52.09%), Postives = 152/215 (70.70%), Query Frame = 0

Query: 9   RKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVEGLHDPPYYE 68
           + PRILCLHGFRTSG IL+  + KWP ++L   D  F+DAPFP+ GKS+VE   DPPYYE
Sbjct: 28  KNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYE 87

Query: 69  WFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQAKGVALTRV 128
           W+Q ++   ++ N EECL +IE++M+++GPFDGLLGFSQGA L A +PG Q +G ALT+V
Sbjct: 88  WYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKV 147

Query: 129 PKIKFVIIINGVKLESPSL------DAQEAYASSIACPSLHFLSEEDFFMPSGLQLLESF 188
           PK+KF++II+G K+  P L       A  A++S + CPSLHF+ E DF    G  L+ESF
Sbjct: 148 PKVKFLVIISGAKI--PGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEVLVESF 207

Query: 189 VEPFVINHLKGHAVPRLDQKSLKIMENFIDRISKL 218
           VEP VI+H  GH +P+LD K+ + M +F   I ++
Sbjct: 208 VEPVVIHHSGGHIIPKLDTKAEETMLSFFQSIRQM 240

BLAST of CmaCh05G005230 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 217.2 bits (552), Expect = 1.3e-56
Identity = 102/166 (61.45%), Postives = 132/166 (79.52%), Query Frame = 0

Query: 1   MGSKSR-IQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVE 60
           MGS+ R I RKPR LCLHGFRTSG I+K  + KWP SV+++ D  F+DAPFP +GKS+VE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GLHDPPYYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQ 120
           G+ DPPYYEWFQF+++  ++TN E+CL+++E+ M++ GPFDGL+GFSQGA L+  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 AKGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFL 166
           AKG+A  +VPKIKFVIII G KL+S  L A+ AY+SS+   SLHFL
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKL-AENAYSSSLETLSLHFL 165

BLAST of CmaCh05G005230 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-11
Identity = 57/187 (30.48%), Postives = 82/187 (43.85%), Query Frame = 0

Query: 8   QRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPF----------PSRGKSE 67
           +RK RILCLHGFR + +  K         + N  +  F+DAP           P  G   
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 68  ------VEGLHDPP----------YYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDG 127
                 V    D P           ++  Q+   T  F   ++ L +++    E GPFDG
Sbjct: 411 KKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGF---DKSLTYLKTAFEEKGPFDG 470

Query: 128 LLGFSQGAALAALLPGFQAKGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPS 169
           +LGFSQGAA+AA + G Q +   L      +F ++ +G     P L+ +E    SI CPS
Sbjct: 471 ILGFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSGF-TPWPLLEMKE--KRSIKCPS 528