CmaCh05G004520 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G004520
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpeptidyl-prolyl cis-trans isomerase E
LocationCma_Chr05: 2073127 .. 2075297 (+)
RNA-Seq ExpressionCmaCh05G004520
SyntenyCmaCh05G004520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAATATTAGGCCCATACTGTCTCATACGGCCCAAGAACGCAACAAACCCAATAGGCCCAAAAACAAGAACAAAAAGCGATCAAGTACAGAGAAGAAATAGGCGTCGACGAAGCAGAAGAATCCTGAAGAAAAGAGCATAAACGCCTCTAATGGCGCAGGCTGTTCAGAAAAATACTCTGTACGTTGGTGGTTTGGCGGAGGAGGTGAACGAGTCTATTCTACACGCAGCCTTCATTCCCTTCGGCGACATCAAAGATGTAAAGACTCCACTGGATCAAGCCACTCAGAAGCACCGTTCCTTCGGCTTCGTCACTTTCTTGGAGAAGGAAGATGCATCCGCCGCCATGGATAATATGGACGGTGCTGAGCTCTACGGCCGTGTCCTCACTGTCAACTATGCCCTCCCCGAGCGTATTAAGGGTGGTGAACAAGGATGGGCTGCCCAACCTAGTAAGTTCCCCTTTTCCTTAAACCCTAATTATTTTCATCTATTGTTTCTCTGCATTTGTGGAATTCCGTTTGTGTTACATCTCATTGTTGATTGATAAATTCTTAGGTGTGCTCGGTTACCAATTTTGCTCTAACTTCATGCTTATGATAGGAGTCCTTGAAAATAGAAATAGAATTTATGCAATCTATTTGGTTGCATTGCATACAATAGTGGTTCGTTGGTTATATTCTCAATCTTCAATTTCATTCTTACAATTTCAAATGTTTTGTTAGTGCAATAAAAAGTTTCAGACTGGCTGATTTCTTATTTGATTTCAAATCAAATACATGTATCTTAAAGCAGAAGTTCGGATACAATACAGAAGTTTAAACAAAGACTGAAAATGATTTATATGCAAAACTCTGTGAAGGACCCGAAGCTAACAATTTAAAAAGTAAACTAAAATTTGGTTCATGTGGAAGTTCTGGACCAAAATTAGATTTAAACAGCTTATCATTTTCTGGTCCCGAAGTTTAAACCTTAGAACAAGATTTGGAAATTATTTTAAGATTTGGACTCTGTCATTTTAGGATTTATTTAGATTATGTTTATGTATCTATATCTCCTGCCTTGGTTGCTAATTACAAGTATTGCAATCTATGGAATTAGCTTGCTGATAGTTTCCTCCATTCCTGCCGATATGAACCTAGCTTAGTAGTTAAAGCATCTATGAAGTTTGAGGTTCAAATTCTCGCAGCTTGTCGTATAAAACAAAAATATGTTCCATCTCCTTGACTGTTCTTTGATTTCTATTTCCCCTCAATGTTCTTGTGGGCTCCTGTATCATCACTACTTGTTCTGCATCTTTTGTGAGCTTAGAATTGAGTGATTAAAGCTTTACTGCACGTCTATTACCGAGTGGCTGGCACTTATGAGAAATTGACTGGTGTAGAGGCCTACTAACTTGAAATTAGCAGCCTCGTTCAGTTCCCATGGATAGTTGTATACTTTGTCTTTCATCATTCCCTTTCCATTTCACTGGACGTTTTTGTATGATTGAAATGTGATGCATTGCAATAGTGATCTTGCAAATGGGTCTGAGTGAGTGATTTTCCCCCTCTTCTTGTCCTAATTGTTACTAAACAAACGCCTTCCTTTAAATTATAGTTTGGGCGGATGCGGATACGTGGTTTGAAAGGCAGCAACAAGAGGAGGAAATGCAGCGGATTCAGGCTGAGAATCGCGCAACTATGCAGGCTGCTGAGGAGCTGCACAGAAAGAAAGTAGCCCAAGAGCGGGAAGGGGAAAAAGAAGACGAGGTAGACACGAAGGACGATCCCATGGCTAAGGCTGAAGCCGAGGCATTAAGACAGAACAGTTAAAGAGACAAACTTTAGTGAGATTTGAACTTGCTTCAATGCAAGTGAAAGAGAGAGATAGAGAGGCAGAACATGGAAAAACCTGCCGTGCTCTGAGCTTGCTATTGCTATTCACAATGCTGATGCATGTTAAGCTTCCGCATTGTTTTGTTTCTGTAGTTAATTCTATTCACAAAGCATAGTTTGAAAAGCTGAAAAACGCTTACCTTCGTTGAAAACTTGTAATTTTATTGAAAGAAGAGCTAGAAATCAGGAGTTTTTAATGCGCAGCATTGTTGTTTTAATTCCCCAATTGGTTTCTTTTAGGGTTGATCCTGGAAGACTCTTCTTTCTTTCAAGATTGAAGAGTTAAGTTTGTCT

mRNA sequence

CCCAATATTAGGCCCATACTGTCTCATACGGCCCAAGAACGCAACAAACCCAATAGGCCCAAAAACAAGAACAAAAAGCGATCAAGTACAGAGAAGAAATAGGCGTCGACGAAGCAGAAGAATCCTGAAGAAAAGAGCATAAACGCCTCTAATGGCGCAGGCTGTTCAGAAAAATACTCTGTACGTTGGTGGTTTGGCGGAGGAGGTGAACGAGTCTATTCTACACGCAGCCTTCATTCCCTTCGGCGACATCAAAGATGTAAAGACTCCACTGGATCAAGCCACTCAGAAGCACCGTTCCTTCGGCTTCGTCACTTTCTTGGAGAAGGAAGATGCATCCGCCGCCATGGATAATATGGACGGTGCTGAGCTCTACGGCCGTGTCCTCACTGTCAACTATGCCCTCCCCGAGCGTATTAAGGGTGGTGAACAAGGATGGGCTGCCCAACCTATTTGGGCGGATGCGGATACGTGGTTTGAAAGGCAGCAACAAGAGGAGGAAATGCAGCGGATTCAGGCTGAGAATCGCGCAACTATGCAGGCTGCTGAGGAGCTGCACAGAAAGAAAGTAGCCCAAGAGCGGGAAGGGGAAAAAGAAGACGAGGTAGACACGAAGGACGATCCCATGGCTAAGGCTGAAGCCGAGGCATTAAGACAGAACAGTTAAAGAGACAAACTTTAGTGAGATTTGAACTTGCTTCAATGCAAGTGAAAGAGAGAGATAGAGAGGCAGAACATGGAAAAACCTGCCGTGCTCTGAGCTTGCTATTGCTATTCACAATGCTGATGCATGTTAAGCTTCCGCATTGTTTTGTTTCTGTAGTTAATTCTATTCACAAAGCATAGTTTGAAAAGCTGAAAAACGCTTACCTTCGTTGAAAACTTGTAATTTTATTGAAAGAAGAGCTAGAAATCAGGAGTTTTTAATGCGCAGCATTGTTGTTTTAATTCCCCAATTGGTTTCTTTTAGGGTTGATCCTGGAAGACTCTTCTTTCTTTCAAGATTGAAGAGTTAAGTTTGTCT

Coding sequence (CDS)

ATGGCGCAGGCTGTTCAGAAAAATACTCTGTACGTTGGTGGTTTGGCGGAGGAGGTGAACGAGTCTATTCTACACGCAGCCTTCATTCCCTTCGGCGACATCAAAGATGTAAAGACTCCACTGGATCAAGCCACTCAGAAGCACCGTTCCTTCGGCTTCGTCACTTTCTTGGAGAAGGAAGATGCATCCGCCGCCATGGATAATATGGACGGTGCTGAGCTCTACGGCCGTGTCCTCACTGTCAACTATGCCCTCCCCGAGCGTATTAAGGGTGGTGAACAAGGATGGGCTGCCCAACCTATTTGGGCGGATGCGGATACGTGGTTTGAAAGGCAGCAACAAGAGGAGGAAATGCAGCGGATTCAGGCTGAGAATCGCGCAACTATGCAGGCTGCTGAGGAGCTGCACAGAAAGAAAGTAGCCCAAGAGCGGGAAGGGGAAAAAGAAGACGAGGTAGACACGAAGGACGATCCCATGGCTAAGGCTGAAGCCGAGGCATTAAGACAGAACAGTTAA

Protein sequence

MAQAVQKNTLYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDASAAMDNMDGAELYGRVLTVNYALPERIKGGEQGWAAQPIWADADTWFERQQQEEEMQRIQAENRATMQAAEELHRKKVAQEREGEKEDEVDTKDDPMAKAEAEALRQNS
Homology
BLAST of CmaCh05G004520 vs. ExPASy Swiss-Prot
Match: A4FV72 (Peptidyl-prolyl cis-trans isomerase E OS=Bos taurus OX=9913 GN=PPIE PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.6e-28
Identity = 60/108 (55.56%), Postives = 79/108 (73.15%), Query Frame = 0

Query: 4   AVQKNTLYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDAS 63
           A  K  LYVGGLAEEV++ +LHAAFIPFGDI D++ PLD  T+KHR F FV F   EDA+
Sbjct: 2   ATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAA 61

Query: 64  AAMDNMDGAELYGRVLTVNYALPERIKGGEQGWAAQPIWADADTWFER 112
           AA+DNM+ +EL+GR + VN A P RIK G    +++P+W+D D W ++
Sbjct: 62  AAIDNMNESELFGRTIRVNLAKPMRIKEG----SSRPVWSD-DDWLKK 104

BLAST of CmaCh05G004520 vs. ExPASy Swiss-Prot
Match: Q9QZH3 (Peptidyl-prolyl cis-trans isomerase E OS=Mus musculus OX=10090 GN=Ppie PE=1 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 5.6e-28
Identity = 60/108 (55.56%), Postives = 79/108 (73.15%), Query Frame = 0

Query: 4   AVQKNTLYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDAS 63
           A  K  LYVGGLAEEV++ +LHAAFIPFGDI D++ PLD  T+KHR F FV F   EDA+
Sbjct: 2   ATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAA 61

Query: 64  AAMDNMDGAELYGRVLTVNYALPERIKGGEQGWAAQPIWADADTWFER 112
           AA+DNM+ +EL+GR + VN A P RIK G    +++P+W+D D W ++
Sbjct: 62  AAIDNMNESELFGRTIRVNLAKPMRIKEG----SSRPVWSD-DDWLKK 104

BLAST of CmaCh05G004520 vs. ExPASy Swiss-Prot
Match: Q5R723 (Peptidyl-prolyl cis-trans isomerase E OS=Pongo abelii OX=9601 GN=PPIE PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 8.0e-27
Identity = 67/139 (48.20%), Postives = 91/139 (65.47%), Query Frame = 0

Query: 4   AVQKNTLYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDAS 63
           A  K  LYVGGLAEEV++ +LHAAFIPFGDI D++ PLD  T+KHR F FV F   EDA+
Sbjct: 2   ATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAA 61

Query: 64  AAMDNMDGAELYGRVLTVNYALPERIKGGEQGWAAQPIWADADTW--------FERQQQE 123
           AA+DNM+ +EL+GR + VN A P RIK G    +++P+W+D D W         E  ++E
Sbjct: 62  AAIDNMNESELFGRTIRVNLAKPMRIKEG----SSRPVWSD-DDWLKKFSGKTLEENKEE 121

Query: 124 EEMQRIQAENRATMQAAEE 135
           E  +  +AE +    AA++
Sbjct: 122 EGSEPPKAETQEGEPAAKK 135

BLAST of CmaCh05G004520 vs. ExPASy Swiss-Prot
Match: Q9UNP9 (Peptidyl-prolyl cis-trans isomerase E OS=Homo sapiens OX=9606 GN=PPIE PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.0e-26
Identity = 68/139 (48.92%), Postives = 91/139 (65.47%), Query Frame = 0

Query: 4   AVQKNTLYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDAS 63
           A  K  LYVGGLAEEV++ +LHAAFIPFGDI D++ PLD  T+KHR F FV F   EDA+
Sbjct: 2   ATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAA 61

Query: 64  AAMDNMDGAELYGRVLTVNYALPERIKGGEQGWAAQPIWADADTW---FERQQQEEEMQR 123
           AA+DNM+ +EL+GR + VN A P RIK G    +++P+W+D D W   F  +  EE  + 
Sbjct: 62  AAIDNMNESELFGRTIRVNLAKPMRIKEG----SSRPVWSD-DDWLKKFSGKTLEENKEE 121

Query: 124 IQAE-NRATMQAAEELHRK 139
             +E  +A  Q  E + +K
Sbjct: 122 EGSEPPKAETQEGEPIAKK 135

BLAST of CmaCh05G004520 vs. ExPASy Swiss-Prot
Match: Q9V3G3 (Peptidyl-prolyl cis-trans isomerase E OS=Drosophila melanogaster OX=7227 GN=cyp33 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 5.4e-23
Identity = 62/141 (43.97%), Postives = 87/141 (61.70%), Query Frame = 0

Query: 7   KNTLYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDASAAM 66
           K T+YVGGLA+EV E +L+ AFIPFGDI D++ P D  +Q+HR F F+ + + EDA+AA+
Sbjct: 5   KRTIYVGGLADEVTERLLNNAFIPFGDIADIQMPADYESQRHRGFAFIEYEQSEDAAAAI 64

Query: 67  DNMDGAELYGRVLTVNYALPERIKGGEQGWAAQPIWADADTWFERQ-----QQEEEMQRI 126
           DNM+ +EL GR + VN A P R+K      + +PIWAD D W ++      Q E E +  
Sbjct: 65  DNMNDSELCGRTIRVNLAKPVRVKED----SFKPIWAD-DDWLQKHAGATLQPEGEPEAE 124

Query: 127 QAENRATMQAAEELHRKKVAQ 143
           + E  +T  A  E   K+  Q
Sbjct: 125 KVETPSTGPAVIEKAEKRNPQ 140

BLAST of CmaCh05G004520 vs. TAIR 10
Match: AT1G13690.1 (ATPase E1 )

HSP 1 Score: 282.7 bits (722), Expect = 1.9e-76
Identity = 138/165 (83.64%), Postives = 158/165 (95.76%), Query Frame = 0

Query: 3   QAVQKNTLYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDA 62
           QA+QKNTLYVGGLA+EVNESILHAAFIPFGDIKDVKTPLDQA QKHRSFGFVTFLE+EDA
Sbjct: 8   QAMQKNTLYVGGLADEVNESILHAAFIPFGDIKDVKTPLDQANQKHRSFGFVTFLEREDA 67

Query: 63  SAAMDNMDGAELYGRVLTVNYALPERIKGGEQGWAAQPIWADADTWFERQQQEEEMQRIQ 122
           SAAMDNMDGAELYGRVLTVNYALPE+IKGGEQGWAA P+WADADTWFERQQQE+E+ ++Q
Sbjct: 68  SAAMDNMDGAELYGRVLTVNYALPEKIKGGEQGWAAHPLWADADTWFERQQQEKEILKMQ 127

Query: 123 AENRATMQAAEELHRKKVAQEREGEKEDEVDTKDDPMAKAEAEAL 168
           AEN+A M+ AEELHRKK+A++R+GE E++ DTK+DPMA+AEA+AL
Sbjct: 128 AENKAAMETAEELHRKKLAEDRQGEMEEDTDTKNDPMARAEADAL 172

BLAST of CmaCh05G004520 vs. TAIR 10
Match: AT5G61030.1 (glycine-rich RNA-binding protein 3 )

HSP 1 Score: 80.1 bits (196), Expect = 1.9e-15
Identity = 38/93 (40.86%), Postives = 59/93 (63.44%), Query Frame = 0

Query: 3   QAVQKNTLYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDA 62
           + +  + L++GG+A  ++E  L  AF  +G++ D +  LD+ T + R FGFVTF   E A
Sbjct: 35  RCMSSSKLFIGGMAYSMDEDSLREAFTKYGEVVDTRVILDRETGRSRGFGFVTFTSSEAA 94

Query: 63  SAAMDNMDGAELYGRVLTVNYALPERIKGGEQG 96
           S+A+  +DG +L+GRV+ VNYA  +R  GG  G
Sbjct: 95  SSAIQALDGRDLHGRVVKVNYA-NDRTSGGGFG 126

BLAST of CmaCh05G004520 vs. TAIR 10
Match: AT3G08000.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.1e-14
Identity = 38/91 (41.76%), Postives = 58/91 (63.74%), Query Frame = 0

Query: 2   AQAVQKNTLYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKED 61
           + A   + L++GGL+  V+E  L  AF  FG++ +V+   D+ + + R FGFV F E+ D
Sbjct: 35  SSASPSSKLFIGGLSWSVDEQSLKDAFSSFGEVAEVRIAYDKGSGRSRGFGFVDFAEEGD 94

Query: 62  ASAAMDNMDGAELYGRVLTVNYALPERIKGG 93
           A +A D MDG  L GR L +++AL ER++GG
Sbjct: 95  ALSAKDAMDGKGLLGRPLRISFAL-ERVRGG 124

BLAST of CmaCh05G004520 vs. TAIR 10
Match: AT4G13850.4 (glycine-rich RNA-binding protein 2 )

HSP 1 Score: 68.9 bits (167), Expect = 4.4e-12
Identity = 37/86 (43.02%), Postives = 53/86 (61.63%), Query Frame = 0

Query: 10  LYVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDASAAMDNM 69
           L++GGL+   +++ L  AF  FGD+ D K  +D+ T + R FGFV F ++  A+AA+  M
Sbjct: 37  LFIGGLSWGTDDASLRDAFAHFGDVVDAKVIVDRETGRSRGFGFVNFNDEGAATAAISEM 96

Query: 70  DGAELYGRVLTVNYALPERIKGGEQG 96
           DG EL GR + VN A  +R  GG  G
Sbjct: 97  DGKELNGRHIRVNPA-NDRPSGGYGG 121

BLAST of CmaCh05G004520 vs. TAIR 10
Match: AT4G39260.3 (cold, circadian rhythm, and RNA binding 1 )

HSP 1 Score: 68.6 bits (166), Expect = 5.7e-12
Identity = 37/86 (43.02%), Postives = 50/86 (58.14%), Query Frame = 0

Query: 11 YVGGLAEEVNESILHAAFIPFGDIKDVKTPLDQATQKHRSFGFVTFLEKEDASAAMDNMD 70
          +VGGLA   N+  L   F  FGD+ D K   D+ + + R FGFVTF +++    A++ M+
Sbjct: 9  FVGGLAWATNDEDLQRTFSQFGDVIDSKIINDRESGRSRGFGFVTFKDEKAMRDAIEEMN 68

Query: 71 GAELYGRVLTVNYALPERIKGGEQGW 97
          G EL GRV+TVN A      GG  GW
Sbjct: 69 GKELDGRVITVNEAQSRGSGGG--GW 92

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A4FV725.6e-2855.56Peptidyl-prolyl cis-trans isomerase E OS=Bos taurus OX=9913 GN=PPIE PE=2 SV=1[more]
Q9QZH35.6e-2855.56Peptidyl-prolyl cis-trans isomerase E OS=Mus musculus OX=10090 GN=Ppie PE=1 SV=2[more]
Q5R7238.0e-2748.20Peptidyl-prolyl cis-trans isomerase E OS=Pongo abelii OX=9601 GN=PPIE PE=2 SV=1[more]
Q9UNP93.0e-2648.92Peptidyl-prolyl cis-trans isomerase E OS=Homo sapiens OX=9606 GN=PPIE PE=1 SV=1[more]
Q9V3G35.4e-2343.97Peptidyl-prolyl cis-trans isomerase E OS=Drosophila melanogaster OX=7227 GN=cyp3... [more]
Match NameE-valueIdentityDescription
AT1G13690.11.9e-7683.64ATPase E1 [more]
AT5G61030.11.9e-1540.86glycine-rich RNA-binding protein 3 [more]
AT3G08000.12.1e-1441.76RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G13850.44.4e-1243.02glycine-rich RNA-binding protein 2 [more]
AT4G39260.35.7e-1243.02cold, circadian rhythm, and RNA binding 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 111..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..171
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 130..163
NoneNo IPR availablePANTHERPTHR15241:SF297PEPTIDYL-PROLYL CIS-TRANS ISOMERASE Ecoord: 3..170
NoneNo IPR availablePANTHERPTHR15241TRANSFORMER-2-RELATEDcoord: 3..170
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 9..82
e-value: 3.2E-23
score: 93.2
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 10..79
e-value: 1.1E-18
score: 66.8
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 8..86
score: 18.188618
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 1..148
e-value: 4.2E-26
score: 93.7
IPR034168Peptidyl-prolyl cis-trans isomerase E, RNA recognition motifCDDcd12347RRM_PPIEcoord: 10..82
e-value: 1.62814E-44
score: 139.665
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 6..95

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G004520.1CmaCh05G004520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016853 isomerase activity
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003723 RNA binding