CmaCh05G003580 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G003580
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionethylene-responsive transcription factor ERF003-like
LocationCma_Chr05: 1604135 .. 1604999 (-)
RNA-Seq ExpressionCmaCh05G003580
SyntenyCmaCh05G003580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTCTCCGCTCACCATGCCCAGACCACAACAAAGATTCCGCGGTGTCCGACAGCGCCATTGGGGCTCATGGGTCTCCGAAATTCGCCATCCTTTATTGTAAGTCCATTCTCCTAGCTTTAAACTAAGTCCATTCGCCATCCTTTTTGTTCACTGATTTTTGTTCATACAGGAAGACTAGAATTTGGTTGGGCACGTTCGAGACGGCCGAAGACGCAGCGCGCGCATACGACGAGGCCGCGCGCCTCATGTGCGGGCCAAGGGCGCGCACTAACTTCCCTTACGACGCCTCCCTGTCGCGCTCCTCATCTTCCCGCCTTCTCTCGGCTACTCTAACCGCCAAGCTTCACCGCTGCTACATGGCCTCCTTGCAACTCACCAAGCAATCCTCCTCTGCTGCACGTCGCCCTCAAACCGACACAATCCGAACCGATTTTTCCGTGACATCCGGCATTGCTCCCGTCGGTGTGGAAGCCGACGTGACGTCAACGAAGAAAGTCAAACTAGAGAGCGTGGATCGCGGGGAGCCGTTGGATGATGATCATATTCAACAAATGATCGAGGAGCTCCTTGATTACGGCGGAATTGAGCTCCGTTCCGCCGCCGGCCTGCAGGCTCAGCCCATTTGACATTCAACTATAGATCTCCGCCTCTAATTATGTACAAAATATGAATTAATTTAGCTAATTTAATTAACTACCCGACTTTTGATCATTTGAGGTCATTATTTCCTCTTGGCCAACCCAGATTTGTTTCTCTCTGGTTTGGCCAATTGAGTGGCGGCGCCCCCATTTTATTTCATGTACTATTTTCCAAATCCCTTTATATTACATAATTAGTATGAAATCCTTATAATTTCATTTCCCC

mRNA sequence

TGTCTCCGCTCACCATGCCCAGACCACAACAAAGATTCCGCGGTGTCCGACAGCGCCATTGGGGCTCATGGGTCTCCGAAATTCGCCATCCTTTATTGAAGACTAGAATTTGGTTGGGCACGTTCGAGACGGCCGAAGACGCAGCGCGCGCATACGACGAGGCCGCGCGCCTCATGTGCGGGCCAAGGGCGCGCACTAACTTCCCTTACGACGCCTCCCTGTCGCGCTCCTCATCTTCCCGCCTTCTCTCGGCTACTCTAACCGCCAAGCTTCACCGCTGCTACATGGCCTCCTTGCAACTCACCAAGCAATCCTCCTCTGCTGCACGTCGCCCTCAAACCGACACAATCCGAACCGATTTTTCCGTGACATCCGGCATTGCTCCCGTCGGTGTGGAAGCCGACGTGACGTCAACGAAGAAAGTCAAACTAGAGAGCGTGGATCGCGGGGAGCCGTTGGATGATGATCATATTCAACAAATGATCGAGGAGCTCCTTGATTACGGCGGAATTGAGCTCCGTTCCGCCGCCGGCCTGCAGGCTCAGCCCATTTGACATTCAACTATAGATCTCCGCCTCTAATTATGTACAAAATATGAATTAATTTAGCTAATTTAATTAACTACCCGACTTTTGATCATTTGAGGTCATTATTTCCTCTTGGCCAACCCAGATTTGTTTCTCTCTGGTTTGGCCAATTGAGTGGCGGCGCCCCCATTTTATTTCATGTACTATTTTCCAAATCCCTTTATATTACATAATTAGTATGAAATCCTTATAATTTCATTTCCCC

Coding sequence (CDS)

ATGCCCAGACCACAACAAAGATTCCGCGGTGTCCGACAGCGCCATTGGGGCTCATGGGTCTCCGAAATTCGCCATCCTTTATTGAAGACTAGAATTTGGTTGGGCACGTTCGAGACGGCCGAAGACGCAGCGCGCGCATACGACGAGGCCGCGCGCCTCATGTGCGGGCCAAGGGCGCGCACTAACTTCCCTTACGACGCCTCCCTGTCGCGCTCCTCATCTTCCCGCCTTCTCTCGGCTACTCTAACCGCCAAGCTTCACCGCTGCTACATGGCCTCCTTGCAACTCACCAAGCAATCCTCCTCTGCTGCACGTCGCCCTCAAACCGACACAATCCGAACCGATTTTTCCGTGACATCCGGCATTGCTCCCGTCGGTGTGGAAGCCGACGTGACGTCAACGAAGAAAGTCAAACTAGAGAGCGTGGATCGCGGGGAGCCGTTGGATGATGATCATATTCAACAAATGATCGAGGAGCTCCTTGATTACGGCGGAATTGAGCTCCGTTCCGCCGCCGGCCTGCAGGCTCAGCCCATTTGA

Protein sequence

MPRPQQRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPYDASLSRSSSSRLLSATLTAKLHRCYMASLQLTKQSSSAARRPQTDTIRTDFSVTSGIAPVGVEADVTSTKKVKLESVDRGEPLDDDHIQQMIEELLDYGGIELRSAAGLQAQPI
Homology
BLAST of CmaCh05G003580 vs. ExPASy Swiss-Prot
Match: Q94AW5 (Ethylene-responsive transcription factor ERF003 OS=Arabidopsis thaliana OX=3702 GN=ERF003 PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 3.3e-55
Identity = 117/182 (64.29%), Postives = 137/182 (75.27%), Query Frame = 0

Query: 1   MPRPQQRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRAR 60
           M RPQQRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRAR
Sbjct: 1   MARPQQRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRAR 60

Query: 61  TNFPYDASLSRSSSSRLLSATLTAKLHRCYMASLQLTKQSSSAARRPQTDTIRTDFSVTS 120
           TNFPY+ +   +SSS+LLSATLTAKLH+CYMASLQ+TKQ+ +   + QT T R+  + + 
Sbjct: 61  TNFPYNPNAIPTSSSKLLSATLTAKLHKCYMASLQMTKQTQT---QTQTQTARSQSADSD 120

Query: 121 GIA--PVGVEADVTSTKKVKLESVDRG-----EPLDDDHIQQMIEELLDYGGIELRSAAG 176
           G+      +   VT T ++K E  +        PL++DHI+QMIEELL YG IEL S   
Sbjct: 121 GVTANESHLNRGVTETTEIKWEDGNANMQQNFRPLEEDHIEQMIEELLHYGSIELCSVLP 179

BLAST of CmaCh05G003580 vs. ExPASy Swiss-Prot
Match: P16146 (Protein PPLZ02 OS=Lupinus polyphyllus OX=3874 GN=PPLZ02 PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.3e-43
Identity = 98/170 (57.65%), Postives = 118/170 (69.41%), Query Frame = 0

Query: 1   MPRPQQRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRAR 60
           M RPQQR+RG RQRHWGSWVSEIRH +LKTRIW GTFE+AEDAARAYDEAARLMCG RAR
Sbjct: 1   MARPQQRYRGFRQRHWGSWVSEIRHSILKTRIWQGTFESAEDAARAYDEAARLMCGTRAR 60

Query: 61  TNFPYDASLSRSSSSRLLSATLTAKLHRCYMASLQLTKQSSSAARRPQTDTIRTDFSVTS 120
           TNFPY+ + S+SSSS+LLSATL AKLHRCYMASLQ+T+ SS        +  R   S  +
Sbjct: 61  TNFPYNPNASQSSSSKLLSATLIAKLHRCYMASLQMTRPSS------LPEAPRIIASPNN 120

Query: 121 GIAPVGVEADV---------------TSTKKVKLESVDRGEPLDDDHIQQ 156
            +  +G +A +                  KKVK+E   + +PL++DHI Q
Sbjct: 121 AVKGIGADAMLLPKKREQEEQETGGNLDFKKVKVECSQQFKPLEEDHIAQ 164

BLAST of CmaCh05G003580 vs. ExPASy Swiss-Prot
Match: Q9XI33 (Ethylene-responsive transcription factor WIN1 OS=Arabidopsis thaliana OX=3702 GN=WIN1 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 4.6e-25
Identity = 83/194 (42.78%), Postives = 110/194 (56.70%), Query Frame = 0

Query: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPY 65
           ++FRGVRQRHWGSWV+EIRHPLLK RIWLGTFETAE+AARAYDEAA LM G  A+TNFP 
Sbjct: 5   KKFRGVRQRHWGSWVAEIRHPLLKRRIWLGTFETAEEAARAYDEAAVLMSGRNAKTNFPL 64

Query: 66  D-----------------ASLSRSSSSRLLSATLTAKLHRC------YMASLQLTKQSSS 125
           +                 +++S S+SS  LS+ L+AKL +C       +  L+L   SS 
Sbjct: 65  NNNNTGETSEGKTDISASSTMSSSTSSSSLSSILSAKLRKCCKSPSPSLTCLRLDTASSH 124

Query: 126 AARRPQTDTIRTDFS--VTSGIAPVGVEADVTS----------TKKVKLESVDRGEPLDD 163
                +    ++D S  +T  + P     + TS          T +V++    R E LD+
Sbjct: 125 IGVWQKRAGSKSDSSWVMTVELGPASSSQETTSKASQDAILAPTTEVEIGG-SREEVLDE 184

BLAST of CmaCh05G003580 vs. ExPASy Swiss-Prot
Match: Q3E958 (Ethylene-responsive transcription factor SHINE 3 OS=Arabidopsis thaliana OX=3702 GN=SHN3 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 3.7e-22
Identity = 77/177 (43.50%), Postives = 100/177 (56.50%), Query Frame = 0

Query: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPY 65
           ++FRGVRQR WGSWVSEIRHPLLK R+WLGTF+TAE AARAYD+AA LM G  A+TNFP 
Sbjct: 5   KKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAETAARAYDQAAVLMNGQSAKTNFPV 64

Query: 66  DASLSRSS--------SSRLLSATLTAKLHR-C-----YMASLQLTKQSSSAARRPQTDT 125
             S   +S        S + LS  L AKL + C     Y+  L+L   SS      +   
Sbjct: 65  IKSNGSNSLEINSALRSPKSLSELLNAKLRKNCKDQTPYLTCLRLDNDSSHIGVWQKRAG 124

Query: 126 IRTDFSVTSGIAPVGVEADV-----TSTKKVKLESVDRGEPLDDDHIQQMIEELLDY 164
            +T  +    +  +G + +        T K+K+ + D  E  DD    QMIEELL++
Sbjct: 125 SKTSPNWVK-LVELGDKVNARPGGDIETNKMKVRNEDVQE--DDQMAMQMIEELLNW 178

BLAST of CmaCh05G003580 vs. ExPASy Swiss-Prot
Match: Q9LFN7 (Ethylene-responsive transcription factor SHINE 2 OS=Arabidopsis thaliana OX=3702 GN=SHN2 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 5.3e-21
Identity = 76/184 (41.30%), Postives = 100/184 (54.35%), Query Frame = 0

Query: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPY 65
           ++FRGVRQR WGSWVSEIRHPLLK R+WLGTFETAE AARAYD+AA LM G  A+TNFP 
Sbjct: 5   RKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFETAEAAARAYDQAALLMNGQNAKTNFPV 64

Query: 66  DASLSRSS----------SSRLLSATLTAKLHRC------YMASLQLTKQSSSAA---RR 125
             S   S           S + LS  L AKL +        +  L+L   SS      +R
Sbjct: 65  VKSEEGSDHVKDVNSPLMSPKSLSELLNAKLRKSCKDLTPSLTCLRLDTDSSHIGVWQKR 124

Query: 126 PQTDTIRT---DFSVTSGIAPVGVEADVTSTKKVKLESVDRGEPL---DDDHI-QQMIEE 164
             + T  T      + + +    V+  +T+  K  +E  +  E     D+D +  +MIEE
Sbjct: 125 AGSKTSPTWVMRLELGNVVNESAVDLGLTTMNKQNVEKEEEEEEAIISDEDQLAMEMIEE 184

BLAST of CmaCh05G003580 vs. TAIR 10
Match: AT5G25190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 216.1 bits (549), Expect = 2.3e-56
Identity = 117/182 (64.29%), Postives = 137/182 (75.27%), Query Frame = 0

Query: 1   MPRPQQRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRAR 60
           M RPQQRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRAR
Sbjct: 1   MARPQQRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRAR 60

Query: 61  TNFPYDASLSRSSSSRLLSATLTAKLHRCYMASLQLTKQSSSAARRPQTDTIRTDFSVTS 120
           TNFPY+ +   +SSS+LLSATLTAKLH+CYMASLQ+TKQ+ +   + QT T R+  + + 
Sbjct: 61  TNFPYNPNAIPTSSSKLLSATLTAKLHKCYMASLQMTKQTQT---QTQTQTARSQSADSD 120

Query: 121 GIA--PVGVEADVTSTKKVKLESVDRG-----EPLDDDHIQQMIEELLDYGGIELRSAAG 176
           G+      +   VT T ++K E  +        PL++DHI+QMIEELL YG IEL S   
Sbjct: 121 GVTANESHLNRGVTETTEIKWEDGNANMQQNFRPLEEDHIEQMIEELLHYGSIELCSVLP 179

BLAST of CmaCh05G003580 vs. TAIR 10
Match: AT1G15360.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 3.3e-26
Identity = 83/194 (42.78%), Postives = 110/194 (56.70%), Query Frame = 0

Query: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPY 65
           ++FRGVRQRHWGSWV+EIRHPLLK RIWLGTFETAE+AARAYDEAA LM G  A+TNFP 
Sbjct: 5   KKFRGVRQRHWGSWVAEIRHPLLKRRIWLGTFETAEEAARAYDEAAVLMSGRNAKTNFPL 64

Query: 66  D-----------------ASLSRSSSSRLLSATLTAKLHRC------YMASLQLTKQSSS 125
           +                 +++S S+SS  LS+ L+AKL +C       +  L+L   SS 
Sbjct: 65  NNNNTGETSEGKTDISASSTMSSSTSSSSLSSILSAKLRKCCKSPSPSLTCLRLDTASSH 124

Query: 126 AARRPQTDTIRTDFS--VTSGIAPVGVEADVTS----------TKKVKLESVDRGEPLDD 163
                +    ++D S  +T  + P     + TS          T +V++    R E LD+
Sbjct: 125 IGVWQKRAGSKSDSSWVMTVELGPASSSQETTSKASQDAILAPTTEVEIGG-SREEVLDE 184

BLAST of CmaCh05G003580 vs. TAIR 10
Match: AT5G25390.2 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 2.6e-23
Identity = 77/177 (43.50%), Postives = 100/177 (56.50%), Query Frame = 0

Query: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPY 65
           ++FRGVRQR WGSWVSEIRHPLLK R+WLGTF+TAE AARAYD+AA LM G  A+TNFP 
Sbjct: 5   KKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAETAARAYDQAAVLMNGQSAKTNFPV 64

Query: 66  DASLSRSS--------SSRLLSATLTAKLHR-C-----YMASLQLTKQSSSAARRPQTDT 125
             S   +S        S + LS  L AKL + C     Y+  L+L   SS      +   
Sbjct: 65  IKSNGSNSLEINSALRSPKSLSELLNAKLRKNCKDQTPYLTCLRLDNDSSHIGVWQKRAG 124

Query: 126 IRTDFSVTSGIAPVGVEADV-----TSTKKVKLESVDRGEPLDDDHIQQMIEELLDY 164
            +T  +    +  +G + +        T K+K+ + D  E  DD    QMIEELL++
Sbjct: 125 SKTSPNWVK-LVELGDKVNARPGGDIETNKMKVRNEDVQE--DDQMAMQMIEELLNW 178

BLAST of CmaCh05G003580 vs. TAIR 10
Match: AT5G11190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 3.7e-22
Identity = 76/184 (41.30%), Postives = 100/184 (54.35%), Query Frame = 0

Query: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPY 65
           ++FRGVRQR WGSWVSEIRHPLLK R+WLGTFETAE AARAYD+AA LM G  A+TNFP 
Sbjct: 5   RKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFETAEAAARAYDQAALLMNGQNAKTNFPV 64

Query: 66  DASLSRSS----------SSRLLSATLTAKLHRC------YMASLQLTKQSSSAA---RR 125
             S   S           S + LS  L AKL +        +  L+L   SS      +R
Sbjct: 65  VKSEEGSDHVKDVNSPLMSPKSLSELLNAKLRKSCKDLTPSLTCLRLDTDSSHIGVWQKR 124

Query: 126 PQTDTIRT---DFSVTSGIAPVGVEADVTSTKKVKLESVDRGEPL---DDDHI-QQMIEE 164
             + T  T      + + +    V+  +T+  K  +E  +  E     D+D +  +MIEE
Sbjct: 125 AGSKTSPTWVMRLELGNVVNESAVDLGLTTMNKQNVEKEEEEEEAIISDEDQLAMEMIEE 184

BLAST of CmaCh05G003580 vs. TAIR 10
Match: AT5G25390.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.2e-20
Identity = 75/177 (42.37%), Postives = 98/177 (55.37%), Query Frame = 0

Query: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPY 65
           ++FRGVRQR WGSWVSEIRHPLL   +WLGTF+TAE AARAYD+AA LM G  A+TNFP 
Sbjct: 5   KKFRGVRQRQWGSWVSEIRHPLL---VWLGTFDTAETAARAYDQAAVLMNGQSAKTNFPV 64

Query: 66  DASLSRSS--------SSRLLSATLTAKLHR-C-----YMASLQLTKQSSSAARRPQTDT 125
             S   +S        S + LS  L AKL + C     Y+  L+L   SS      +   
Sbjct: 65  IKSNGSNSLEINSALRSPKSLSELLNAKLRKNCKDQTPYLTCLRLDNDSSHIGVWQKRAG 124

Query: 126 IRTDFSVTSGIAPVGVEADV-----TSTKKVKLESVDRGEPLDDDHIQQMIEELLDY 164
            +T  +    +  +G + +        T K+K+ + D  E  DD    QMIEELL++
Sbjct: 125 SKTSPNWVK-LVELGDKVNARPGGDIETNKMKVRNEDVQE--DDQMAMQMIEELLNW 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AW53.3e-5564.29Ethylene-responsive transcription factor ERF003 OS=Arabidopsis thaliana OX=3702 ... [more]
P161461.3e-4357.65Protein PPLZ02 OS=Lupinus polyphyllus OX=3874 GN=PPLZ02 PE=2 SV=1[more]
Q9XI334.6e-2542.78Ethylene-responsive transcription factor WIN1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q3E9583.7e-2243.50Ethylene-responsive transcription factor SHINE 3 OS=Arabidopsis thaliana OX=3702... [more]
Q9LFN75.3e-2141.30Ethylene-responsive transcription factor SHINE 2 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
AT5G25190.12.3e-5664.29Integrase-type DNA-binding superfamily protein [more]
AT1G15360.13.3e-2642.78Integrase-type DNA-binding superfamily protein [more]
AT5G25390.22.6e-2343.50Integrase-type DNA-binding superfamily protein [more]
AT5G11190.13.7e-2241.30Integrase-type DNA-binding superfamily protein [more]
AT5G25390.11.2e-2042.37Integrase-type DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 30..46
score: 70.91
coord: 8..19
score: 61.21
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 7..70
e-value: 2.0E-39
score: 147.0
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 7..56
e-value: 1.9E-11
score: 44.0
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 7..64
score: 20.718536
IPR001471AP2/ERF domainCDDcd00018AP2coord: 6..66
e-value: 1.55509E-33
score: 111.595
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 6..66
e-value: 4.5E-26
score: 92.8
NoneNo IPR availablePANTHERPTHR31194:SF112ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF003coord: 1..172
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 1..172
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 7..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G003580.1CmaCh05G003580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity