CmaCh05G002660 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G002660
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionTLC domain-containing protein
LocationCma_Chr05: 1127948 .. 1129725 (+)
RNA-Seq ExpressionCmaCh05G002660
SyntenyCmaCh05G002660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTATTGACTCTTCTCTCTCATTGTTAAGCTTTCTACATCTCTGATCTTTCCACAAGCTCTGCTATCTTGGAGCAGCCATGGTGGTCTCCACGGGTCGAGATCCCGACGTAATAAACGTCGTCGTTTTGGGTGTAATAGCTTGGACATTGGCTTTCATTTCCTTGAGAAAACTCCTTCCAAAGAGATCCTTTGGGTTCTGCAACCGCCTCGTCTCCACGCTCCATGCTTGTTTGGCTGTCACCTTGGCTTCCATCTCTGTGCAGAATTGGCGATGCCCCATTTGTCCTCTCGCTTCTCAAGCTTCACCCTTTCAGGTACTTTCTTCTTCAAATCTTTGTACGACGCCGTTTTGTTGCTCTTAATGAATTAGATATGAAATGGGGTTTTTTTAAGCTGTTAAAAATGGTGGATTTGTTCGCCAGATGCAAACATTGGCTGTGACCTGTTCTTATTTGATCTATGATATGGTGTGTTGCCACTTTGACAAGAAAGTGAATGTGGACAATGCCATCCATCACTTGGTTAGCATTGTTGGAATTGCAGCTGGCTTTGCTTATCAAAAGGTACTCTTTTTTTTCTCGTTCAATAAATTAGGCTCGGTTTCAACTATTTATGCTTCGAAGAAGAGGAGAAATCTTCTTTTCTTAGCACGGGTGAGTTAACTATCATGGGTTTGGGTCAAGTTGGTTGAATCACCTACTTAAGATATGGTATCTTACGAGTTACATTGATGATTTCATCGTCGTGAACATGTAGTGTGGGTCAGAGATGGTAGCAGCGTTATGGATAACCGAGATCTCAAGCCCCTTTCTCCATTTAAGGGAAATTCTCAAAGAAATTGGTTACAGAGGCACAGACTTTAATTTGGCCGCTGATGTAAGTCATCGCCCCAATTGATCAAGATTATATGCTTAAATCTAAAAAAATATATTCTAATGAATATCAAACGAAACTTAAGCTAATATGATATTGATTCGATGACAGTTCGGATTTGCTGTGATATTTTCAATTGGAAGAATGGTTGGAGGGCCTTATCTCACCTACATGACTCTATCCGCCACCGCCGTCCCTCTTCTTATTAAGGTAGCAAACATCTAAATGCATATTCTCCATAAAATTTAGTCGTGAATAGATAAAATGTCAATGTCAATAGATATTTTAATAAAAATATCACGAGCTCATAAATAACGAGTTCTTTATGATCACAAGCTCGTAAATAACGAGTTCTTTATGATCATGAGCTCGTAAATACCTTTATCAACTACTTTGTTCAACTTTCACTAACCAAATAGTTCATATAATTCACAGGCCATGGCTCTAGGATTGCAGCTTGTCAGTGCCTACTGGTTTTACAAGATCGTGAGAATGGTCAGATTCAAACTCAACGACAGATCTACATCGAAGAACAGTTAATCTCAGCTAAGGAATCAACAAAAAGATAGAACTCTTACAATTTTATGTTCATCAAATCCCAGCAGTATCTTGGGAACAGTGACACTGCAAACAAAAACAGTGCAAGAACTTTGCAATGAACTGTGTATTCTGTAAACTGAGCTGCAGATGATCATGTTTAGATTAATGAAAATCACAATGAACCATTTCATCTCAGCTTTATATCTTTTCAATGAGCACATTAACAAGAAAGATTTAAGGCAGGGTGAATAAAAATCTGTTTAGGCCACAAGAACAGGGAATAAAATAAGGAAGAAAGAAGAATGGCTAGATTTTCATGAATGTTCATATCATCGTCTGTGATCAGATACAACAGGTTCCTAT

mRNA sequence

ACCTATTGACTCTTCTCTCTCATTGTTAAGCTTTCTACATCTCTGATCTTTCCACAAGCTCTGCTATCTTGGAGCAGCCATGGTGGTCTCCACGGGTCGAGATCCCGACGTAATAAACGTCGTCGTTTTGGGTGTAATAGCTTGGACATTGGCTTTCATTTCCTTGAGAAAACTCCTTCCAAAGAGATCCTTTGGGTTCTGCAACCGCCTCGTCTCCACGCTCCATGCTTGTTTGGCTGTCACCTTGGCTTCCATCTCTGTGCAGAATTGGCGATGCCCCATTTGTCCTCTCGCTTCTCAAGCTTCACCCTTTCAGATGCAAACATTGGCTGTGACCTGTTCTTATTTGATCTATGATATGGTGTGTTGCCACTTTGACAAGAAAGTGAATGTGGACAATGCCATCCATCACTTGGTTAGCATTGTTGGAATTGCAGCTGGCTTTGCTTATCAAAAGTGTGGGTCAGAGATGGTAGCAGCGTTATGGATAACCGAGATCTCAAGCCCCTTTCTCCATTTAAGGGAAATTCTCAAAGAAATTGGTTACAGAGGCACAGACTTTAATTTGGCCGCTGATTTCGGATTTGCTGTGATATTTTCAATTGGAAGAATGGTTGGAGGGCCTTATCTCACCTACATGACTCTATCCGCCACCGCCGTCCCTCTTCTTATTAAGGCCATGGCTCTAGGATTGCAGCTTGTCAGTGCCTACTGGTTTTACAAGATCGTGAGAATGGTCAGATTCAAACTCAACGACAGATCTACATCGAAGAACAGTTAATCTCAGCTAAGGAATCAACAAAAAGATAGAACTCTTACAATTTTATGTTCATCAAATCCCAGCAGTATCTTGGGAACAGTGACACTGCAAACAAAAACAGTGCAAGAACTTTGCAATGAACTGTGTATTCTGTAAACTGAGCTGCAGATGATCATGTTTAGATTAATGAAAATCACAATGAACCATTTCATCTCAGCTTTATATCTTTTCAATGAGCACATTAACAAGAAAGATTTAAGGCAGGGTGAATAAAAATCTGTTTAGGCCACAAGAACAGGGAATAAAATAAGGAAGAAAGAAGAATGGCTAGATTTTCATGAATGTTCATATCATCGTCTGTGATCAGATACAACAGGTTCCTAT

Coding sequence (CDS)

ATGGTGGTCTCCACGGGTCGAGATCCCGACGTAATAAACGTCGTCGTTTTGGGTGTAATAGCTTGGACATTGGCTTTCATTTCCTTGAGAAAACTCCTTCCAAAGAGATCCTTTGGGTTCTGCAACCGCCTCGTCTCCACGCTCCATGCTTGTTTGGCTGTCACCTTGGCTTCCATCTCTGTGCAGAATTGGCGATGCCCCATTTGTCCTCTCGCTTCTCAAGCTTCACCCTTTCAGATGCAAACATTGGCTGTGACCTGTTCTTATTTGATCTATGATATGGTGTGTTGCCACTTTGACAAGAAAGTGAATGTGGACAATGCCATCCATCACTTGGTTAGCATTGTTGGAATTGCAGCTGGCTTTGCTTATCAAAAGTGTGGGTCAGAGATGGTAGCAGCGTTATGGATAACCGAGATCTCAAGCCCCTTTCTCCATTTAAGGGAAATTCTCAAAGAAATTGGTTACAGAGGCACAGACTTTAATTTGGCCGCTGATTTCGGATTTGCTGTGATATTTTCAATTGGAAGAATGGTTGGAGGGCCTTATCTCACCTACATGACTCTATCCGCCACCGCCGTCCCTCTTCTTATTAAGGCCATGGCTCTAGGATTGCAGCTTGTCAGTGCCTACTGGTTTTACAAGATCGTGAGAATGGTCAGATTCAAACTCAACGACAGATCTACATCGAAGAACAGTTAA

Protein sequence

MVVSTGRDPDVINVVVLGVIAWTLAFISLRKLLPKRSFGFCNRLVSTLHACLAVTLASISVQNWRCPICPLASQASPFQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVSIVGIAAGFAYQKCGSEMVAALWITEISSPFLHLREILKEIGYRGTDFNLAADFGFAVIFSIGRMVGGPYLTYMTLSATAVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNDRSTSKNS
Homology
BLAST of CmaCh05G002660 vs. ExPASy Swiss-Prot
Match: Q6ZRR5 (TLC domain-containing protein 5 OS=Homo sapiens OX=9606 GN=TLCD5 PE=2 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 1.5e-12
Identity = 55/205 (26.83%), Postives = 96/205 (46.83%), Query Frame = 0

Query: 15  VVLGVIAWTLAFISLRKLLPKRSFGFCNRLVSTLHACLAVTL-ASISVQNWRCPICPLAS 74
           V+  +  W   +IS   L   RS+ +  RLV+  H  L++ L A I   +   P     S
Sbjct: 9   VLCSLCGWLSLYISFCHLNKHRSYEWSCRLVTFTHGVLSIGLSAYIGFIDGPWPFTHPGS 68

Query: 75  QASPFQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVSIVGIAAGFAYQKCGSEMVA 134
             +P Q+  L +T  Y I+D+  C + +        HH +SI+GI       + G+E+ A
Sbjct: 69  PNTPLQVHVLCLTLGYFIFDLGWCVYFQSEGALMLAHHTLSILGIIMALVLGESGTEVNA 128

Query: 135 ALWITEISSPFLHLREILKEIGYRGTDFNLAADFGFAVIFSIGRMVGGPYLTYMTLSATA 194
            L+ +E+++P L +R  L+E G+  +      DF F  +F+  R+  G  L +  + +  
Sbjct: 129 VLFGSELTNPLLQMRWFLRETGHYHSFTGDVVDFLFVALFTGVRIGVGACLLFCEMVSPT 188

Query: 195 VPLLIKAMALGLQLVSAYWFYKIVR 219
               +KA  + +  VS  + + I R
Sbjct: 189 PKWFVKAGGVAMYAVSWCFMFSIWR 213

BLAST of CmaCh05G002660 vs. ExPASy Swiss-Prot
Match: Q0VD42 (TLC domain-containing protein 5 OS=Bos taurus OX=9913 GN=TLCD5 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.0e-12
Identity = 53/198 (26.77%), Postives = 94/198 (47.47%), Query Frame = 0

Query: 22  WTLAFISLRKLLPKRSFGFCNRLVSTLHACLAVTL-ASISVQNWRCPICPLASQASPFQM 81
           W   ++S  +L   RS+ +  RLV+  H  L++ L A I   +   P     S  +P Q+
Sbjct: 16  WLSLYVSFCRLNKHRSYEWNCRLVTFTHGILSIGLSAYIGFIDGPWPFTHPGSPNTPLQV 75

Query: 82  QTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVSIVGIAAGFAYQKCGSEMVAALWITEI 141
             L +T  Y I+D+  C + +        HH +SI+GI       + G+E+ A L+ +EI
Sbjct: 76  HVLCLTLGYFIFDLGWCIYFRSEGPLMLAHHTLSILGIIVALVLGESGTEVNAVLFGSEI 135

Query: 142 SSPFLHLREILKEIGYRGTDFNLAADFGFAVIFSIGRMVGGPYLTYMTLSATAVPLLIKA 201
           ++P L +R  L+E G+  +      DF F  +F+  R+  G  L +  + +      +K 
Sbjct: 136 TNPLLQIRWFLRETGHYHSFTGDVVDFLFVALFTGVRIGVGACLLFCEMVSPTPKWFVKV 195

Query: 202 MALGLQLVSAYWFYKIVR 219
             + + +VS  + + I R
Sbjct: 196 GGVAMYVVSWCFMFSIWR 213

BLAST of CmaCh05G002660 vs. ExPASy Swiss-Prot
Match: Q3TYE7 (TLC domain-containing protein 5 OS=Mus musculus OX=10090 GN=Tlcd5 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.4e-11
Identity = 54/203 (26.60%), Postives = 94/203 (46.31%), Query Frame = 0

Query: 15  VVLGVIAWTLAFISLRKLLPKRSFGFCNRLVSTLHACLAVTL-ASISVQNWRCPICPLAS 74
           V+  +  W   + S   L   RS  +  RLV+  H  L++ L A I   +   P     S
Sbjct: 9   VLCSLGGWLSLYTSFCCLNKHRSCEWSCRLVTFTHGVLSIGLSAYIGFIDGPWPFTHPGS 68

Query: 75  QASPFQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVSIVGIAAGFAYQKCGSEMVA 134
             +P Q+  L +T  Y I+D+  C + +        HH +SI+GI    A  + G+E+ A
Sbjct: 69  PNTPLQVHVLCLTLGYFIFDLGWCIYFQSEGPLMLAHHTLSILGIIMALALGESGTEVNA 128

Query: 135 ALWITEISSPFLHLREILKEIGYRGTDFNLAADFGFAVIFSIGRMVGGPYLTYMTLSATA 194
            L+ +EI++P L +R  L+E G+  +      DF F  +F+  R+  G +L +  + +  
Sbjct: 129 VLFGSEITNPLLQMRWFLRETGHYHSFTGDVVDFLFVALFTGVRIGVGAHLLFCEMVSPT 188

Query: 195 VPLLIKAMALGLQLVSAYWFYKI 217
               +K   + +  VS  W + +
Sbjct: 189 PKWFVKVGGVAMYAVS--WCFMV 209

BLAST of CmaCh05G002660 vs. ExPASy Swiss-Prot
Match: Q1LXV8 (TLC domain-containing protein 5 OS=Danio rerio OX=7955 GN=tlcd5 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.4e-10
Identity = 52/201 (25.87%), Postives = 90/201 (44.78%), Query Frame = 0

Query: 19  VIAWTLAFISLRKLLPKRSFGFCNRLVSTLHACLAVTL-ASISVQNWRCPICPLASQASP 78
           +I+W   ++ L  L   R   +  RLV+  H  L + L A I       P     ++ + 
Sbjct: 12  LISWLFLYLLLCHLNSGRDSEWNCRLVTLFHGILIICLTAYIGFIAGPWPFTHPGTENTY 71

Query: 79  FQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVSIVGIAAGFAYQKCGSEMVAALWI 138
           FQ+ TL ++  Y ++DM  C + +        HH +SI GI       + G E  A L+ 
Sbjct: 72  FQILTLVLSLGYFLFDMAWCVYFRTEGPVMLAHHTMSIFGILLALGLGESGIETCAVLFG 131

Query: 139 TEISSPFLHLREILKEIGYRGTDFNLAADFGFAVIFSIGRMVGGPYLTYMTLSATAVPLL 198
           +EI++P L  R  LK +G   +      D  F ++F+  R+  G  + Y  L++    L+
Sbjct: 132 SEITNPLLQARWFLKRMGCYDSLAGDVVDLLFILLFASVRIGVGSRMLYCELTSPKPSLI 191

Query: 199 IKAMALGLQLVSAYWFYKIVR 219
           +K   + +  +S  +   I R
Sbjct: 192 VKVGGVAIYTLSWIFMVDIAR 212

BLAST of CmaCh05G002660 vs. TAIR 10
Match: AT1G35180.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 294.3 bits (752), Expect = 8.7e-80
Identity = 143/220 (65.00%), Postives = 179/220 (81.36%), Query Frame = 0

Query: 11  VINVVVLGVIAWTLAFISLRKLLPKRSFGFCNRLVSTLHACLAVTLASISVQNWRCPICP 70
           VI++  +GVI+W L FI +R++    SF F  R+VSTLHA +AVTLA++S+Q+W CP+CP
Sbjct: 8   VISITTIGVISWGLIFILVRRIFSSYSFDFSTRIVSTLHATIAVTLATLSIQDWSCPVCP 67

Query: 71  LASQASPFQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVSIVGIAAGFAYQKCGSE 130
           +AS +S  QM+TLA + SY+IYD++C HFD+ +++DNA+HH V I+G  AG  YQKCGSE
Sbjct: 68  IASTSSLRQMETLAFSLSYMIYDLICSHFDQVLSIDNAVHHSVCILGFVAGLFYQKCGSE 127

Query: 131 MVAALWITEISSPFLHLREILKEIGYRGTDFNLAADFGFAVIFSIGRMVGGPYLTYMTLS 190
           MVAALWITEISSPFLHLREILKEIGYR TD NLAAD  FA IFS+ RMVGGPYL Y+T+S
Sbjct: 128 MVAALWITEISSPFLHLREILKEIGYRDTDLNLAADVCFATIFSLARMVGGPYLVYVTIS 187

Query: 191 ATAVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNDRSTS 231
           A   P+LIKAMALGLQLVSA+WFYKI++M+R+K   RS S
Sbjct: 188 ADN-PILIKAMALGLQLVSAFWFYKILKMMRYKFIKRSMS 226

BLAST of CmaCh05G002660 vs. TAIR 10
Match: AT1G35170.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 270.8 bits (691), Expect = 1.0e-72
Identity = 133/220 (60.45%), Postives = 172/220 (78.18%), Query Frame = 0

Query: 11  VINVVVLGVIAWTLAFISLRKLLPKRSFGFCNRLVSTLHACLAVTLASISVQNWRCPICP 70
           +I++  + +I+W L FI +R++    SF F  R+VSTLHA +AVTLA++S+Q+W CP+CP
Sbjct: 8   IISITTIRIISWGLIFILVRRIFSSYSFDFSTRIVSTLHATIAVTLATLSIQDWSCPVCP 67

Query: 71  LASQASPFQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVSIVGIAAGFAYQKCGSE 130
           +AS +S  QM+TLA + SY+IYD++C HFD+ +++DNA+HH V I+G  AG  YQKCGSE
Sbjct: 68  IASTSSLRQMETLAFSLSYMIYDLICSHFDQVLSIDNAVHHSVCILGFVAGLFYQKCGSE 127

Query: 131 MVAALWITEISSPFLHLREILKEIGYRGTDFNLAADFGFAVIFSIGRMVGGPYLTYMTLS 190
           MVAA+WITEISSPFLHLREILKEIG R TD NLAAD  FA IFS+ RMVGGPYL Y+T+ 
Sbjct: 128 MVAAIWITEISSPFLHLREILKEIGCRDTDLNLAADVFFATIFSVARMVGGPYLVYVTIP 187

Query: 191 ATAVPLLIKAMALGLQLVSAYWFYKIVRMVRFKLNDRSTS 231
           A   P+LIK M LGLQLVSA  FYK+++M+R+K   RS S
Sbjct: 188 ADN-PILIKVMGLGLQLVSA--FYKLLKMMRYKFIKRSMS 224

BLAST of CmaCh05G002660 vs. TAIR 10
Match: AT1G45010.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 261.2 bits (666), Expect = 8.2e-70
Identity = 127/213 (59.62%), Postives = 163/213 (76.53%), Query Frame = 0

Query: 12  INVVVLGVIAWTLAFISLRKLLPKRSFGFCNRLVSTLHACLAVTLASISVQNWRCPICPL 71
           I ++V GVI+W +AFI  R++    SF F NRL+ST HA +AVTLA++SVQ+  CP+CPL
Sbjct: 9   IKIIVFGVISWGVAFILTRRIFSSYSFSFSNRLLSTAHATIAVTLATLSVQDLSCPVCPL 68

Query: 72  ASQASPFQMQTLAVTCSYLIYDMVCCHFDKKVNVDNAIHHLVSIVGIAAGFAYQKCGSEM 131
           AS+ S  QM  +A + SY+IYD++CCHFD+  ++DNA+HH VSI+G  AG AYQK GSE+
Sbjct: 69  ASKPSHKQMDVMAFSLSYMIYDLICCHFDQVFSIDNAVHHFVSILGFIAGLAYQKSGSEI 128

Query: 132 VAALWITEISSPFLHLREILKEIGYRGTDFNLAADFGFAVIFSIGRMVGGPYLTYMTLSA 191
           VA LW+ EISSPF HLREILKEIGY+ T  NLAAD  FA IF++ R+V GP+L Y++LSA
Sbjct: 129 VATLWVAEISSPFFHLREILKEIGYKDTKLNLAADVCFATIFTLARIVCGPFLVYVSLSA 188

Query: 192 TAVPLLIKAMALGLQLVSAYWFYKIVRMVRFKL 225
              P+ IKAM  GLQLVS +WFYKI  M+R+KL
Sbjct: 189 DN-PIFIKAMGSGLQLVSIFWFYKIFGMMRYKL 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6ZRR51.5e-1226.83TLC domain-containing protein 5 OS=Homo sapiens OX=9606 GN=TLCD5 PE=2 SV=2[more]
Q0VD422.0e-1226.77TLC domain-containing protein 5 OS=Bos taurus OX=9913 GN=TLCD5 PE=2 SV=1[more]
Q3TYE78.4e-1126.60TLC domain-containing protein 5 OS=Mus musculus OX=10090 GN=Tlcd5 PE=2 SV=1[more]
Q1LXV85.4e-1025.87TLC domain-containing protein 5 OS=Danio rerio OX=7955 GN=tlcd5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G35180.18.7e-8065.00TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
AT1G35170.11.0e-7260.45TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
AT1G45010.18.2e-7059.62TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006634TRAM/LAG1/CLN8 homology domainSMARTSM00724lag1_27coord: 35..225
e-value: 4.0E-37
score: 139.3
IPR006634TRAM/LAG1/CLN8 homology domainPFAMPF03798TRAM_LAG1_CLN8coord: 40..218
e-value: 2.2E-22
score: 79.8
IPR006634TRAM/LAG1/CLN8 homology domainPROSITEPS50922TLCcoord: 35..225
score: 35.116684
IPR042512TLC domain-containing protein 5PANTHERPTHR31898TRANSMEMBRANE PROTEIN 136coord: 10..230
NoneNo IPR availablePANTHERPTHR31898:SF5SUBFAMILY NOT NAMEDcoord: 10..230

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G002660.1CmaCh05G002660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane