CmaCh05G001210 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G001210
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionSqualene monooxygenase
LocationCma_Chr05: 522621 .. 526730 (-)
RNA-Seq ExpressionCmaCh05G001210
SyntenyCmaCh05G001210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAGAATGTAAATTAGTTGGGAGTTGGGAGATAACTGAGAGTAATTTAGGAGAGATGCGGATGTTAAGCATCGCAAGCTAGTATTAGGCGCCACATTTGAGCTGTAACTAATTATATCCTATGCGACTGTTCATTCAAGGAGACGGGACGGCACCAATGTATAATGTGGCTTCCTTTTCACCACACACATTCTATAAAATCATCCCCTCTTCCTCAATTTTCACTCCCCTTAACCGAATCTTCTAGACTGACCAATAATCTTTCGTCGTCGTCGTCGTCGCCGCTGCGGTGATCTCCGTGTGGTTGCTGGGATTTTGCATTCTCTGGAACGAGGAAACAATGGTTGATTACTGCGCCTTTGGCTGGATCTTGGCCGCCGTCCTCGGCCTTGCAATTGCACTCTCTTTCTTCGTCTCTCCTAGGAGGAATCGTCGAGGAGGAGCCGATTCCACGCCGAGAAGCGAGGGTGTCAGAAGCTCTTCAACTACCAACGGAGAATGCAGATCCGTCGACGGCGACGCCGACGTTATCATCGTCGGAGCAGGTGTTGCCGGTTCCGCTCTTGCTCACACTCTCGGCAAGGTACATCGGTTTTATTGCTTTCTTTTGAATTTTGAATCTGTTCTGTACTCCTGTTCGCTCTCTCTCTCTCTCTCTCTCTCTGCATTTTTCTCCGTCCTGTTCGCGCCACCGCGCATCTAATTACTCCTTCTGGCTCCTATTATTAATGAAAACTTGGGGTATTTGCGTGCTGTTTATATTTGATGCATAGAAACCCTCAGAATGAAAGAATCCATCGCAGAGTTTTGGATTATGGATGAGAGGAAGAAAAATGGAAAGAAGAAAGATATTACTTCGGGATGTCAATTGCTTGTACATGTAACCAATGAAATGACTGGATGATCGTTTTTCTGTCGTTTTCCCCCTTATAACTTGTTATTGGATGAAAGTTTTATGTCGCCACCGGATGATTTTTATATGGATAAATGGAGTGCTTCAATTTCGTTGTAAAATGGTTTTGATTATTGCTGACTTAAGCAGAAGGTTCGAATGTATATTTTAAATTTTAACCCATCTATGTAATTGTTGTTCGATCTTCGGTGAATAACTATATGAATTTCCTTTTGGTAAATAAGTTAATTATTGGGGTTGGATTGTTTATATCCACATCCCTCTTCTTATTGGTTTTATTTATTTATTTATTTATTTTTCTATTTTTGTTGTTCCTCATATTTCACTTTGGAACCAAGCTGATCATATCGAATTTGATAGGATGGTCGTCTAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGAATCGTCGGTGAATTATTGCAGCCTGGGGGCTACCTCAAATTGATTGAGTTAGGACTGCAAGGTTTGTAATTGGCCTGGGGAAATTTCTTTTACAACTCTATTTTCTTTCGAAGTACAATATTCATCAGTAATTAAGGAAAATAAATGTTTGTATTGCAGATTGTGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCCCTTTTCAAGGATGGAAAGAACACTCAACTTTCTTACCCATTGGAAAAATTTCAGTCTGATGTATCTGGAAGAAGCTTCCACAATGGACGCTTCATACAGAGAATGAGGGAGAAGGCTGCTTCCCTTCCCAAGTATGTTCATTTCGTCTTTGTTTTCAACAGAAGATGTTACGAATGTCCCATGGTCCGATCTTTTAAAGTAATGATTCAAGCACTATTTTATATTTCCTTACTACATAAGTAAATGGATTTAAGACAAATTTCTTTGTTTGGTTTATAGCTGCCCTAACCTATCGTTAACAGTATTGTCCTCTTTCCATCTTCCTTTTCAACTTTTTTCTCAAAGTTTTTAAAAGCGTTTGCGAAGGAGAGGTTTCCACGCCCTTATAAGGAATGCTTCGTTCCTCTCTCCAACCGATGTAGAATCTTACAATCTACTCCCCTTGGGAGCCTAGCTTCCTCGCCTGGCACACTGCCCCGGTCTAGCTTTGATACCATTTGTAACAGCCCATGCCCCGCCAGCAGACATTGTTCTTTTGGCTTTCTTAGCTTCCCCTCAAGATTTTTAAAACGCGTCTATTAGGGAGAAGTTTCCACACTCTTGTAAGGAATGCAATCTGGTTACAGTGAGTTCAACAATCGCCATTGATTCACGATCGATACTTCAAGGCTGTGTTTCTTGTGATTGGATAATGAATAACTACTTGGATTTGACTCATGAATGTGACTTTTTATTTGCTCTGTTTCCATTTCTTGTAGTGTAAGATTGGAGCAAGGAACAGTTACTTCGTTGCTTGAAGAAAAGGGAACGATCAAAGGTGTGCAGTATAAGTCTAAAAATGGTGAAGAAAAAACAGCGTATGCACCTCTGACAATTGTTTGTGATGGGTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAAGCCTATGGTAAGGAGGCTTCAATTATTCAGTGGTACCTCAAAATAGACATTGATTTTCTCTTTAGGATTTGATTGGCTGCAGAATTATTCTAATTTCATTTTGCTAACTGCATGAAATTATCGAATACGTTCTTCAAATTCCGAGCCCTTTATTATCTCTATCTTTTGCAGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCATGGACATGTTGTTCTCGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGTAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGATTCCTTCTATATCAAATGGTGAAATGGAAAAGTATTTGAAGACCATAGTAGCTCCTCAGGTACAATTTTTTCAACAGTCCCATTCATGCCAACTTTCATCTTAACATTCATGTGTTAAATTTAGTGCTTCGAACTAATGAATTACCATTACTCGTGCAGGTTCCTCCGCAAATCCATGATGCCTTTATAGCTGCTATTGACAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCTGCTCCCCAACCGACGCCCGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCCCTCACCGGTGGAGGAATGACAGTTGCTTTGTCTGATATTGTTGTGCTCAGGAACCTTCTCAAGCCACTGAAGGACTTGAATGATGCACCAACTCTCTGCAAGTATCTAGAATCCTTTTACACTCTGCGAAAGGTATAGTTTTAGTTTATGTTTGCTTTTTCCTAAAAGCAATTTCACAGAAGGCCATTAGTAAAGCTAATTTATTCGTCTCAAACTGCAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTCTTTTGTGCATCGCCTGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCCGTGTCCTTGCTCTCGGGATTGAATCCTCGCCCATTAAGTCTGGTTCTCCATTTCTTTGCAGTGGCAATATACGGAGTTGGTCGCTTACTGCTTCCATTTCCTTCTCCAAAAGGCATCTGGATTGGAGCAAGATTGGTCTATGTGAGTTATGAACAGATGAAACAGAACAAAACACCTTCCATTCATATTTTCCTTTGAAAACTTTAAGAACTTCGTAGCGGAAGTGTTGTAATGAGAAATGAATTGATTTGTTGCAGAGCGCATCAGGAATCATATTTCCGATTATCAAGGCGGAAGGAGTTAGACAGATGTTCTTCCCTGCTACTGTTCCTGCTTATTACAGAAGCCCACCAGTTCATAAAAGCATAGCATGAAGTGATGGTGATACACAAAGGATCGAACTGGGCATTTGTTTGTATCTACCTGAGGAGGAAGAAGAGGCATCCTCAGGCTGTCGGGCATTGCAGGGAAGACAAATCACTTTTACGTTTGTTAGATTTTCATTTTAATTGTGGATTAATAAACCTGTCTATTTTTGAACAGGAGCCAGGAATATGTAAATGTTCTTAACTCGTTCTAGAAACCCCAACATTAATGTATTTTCAAACAACTTCGGAACTTTCAACATAGTAATACCTAAAACAATCAATTTAGTACTCACCATTACCTTGT

mRNA sequence

ATAGAATGTAAATTAGTTGGGAGTTGGGAGATAACTGAGAGTAATTTAGGAGAGATGCGGATGTTAAGCATCGCAAGCTAGTATTAGGCGCCACATTTGAGCTGTAACTAATTATATCCTATGCGACTGTTCATTCAAGGAGACGGGACGGCACCAATGTATAATGTGGCTTCCTTTTCACCACACACATTCTATAAAATCATCCCCTCTTCCTCAATTTTCACTCCCCTTAACCGAATCTTCTAGACTGACCAATAATCTTTCGTCGTCGTCGTCGTCGCCGCTGCGGTGATCTCCGTGTGGTTGCTGGGATTTTGCATTCTCTGGAACGAGGAAACAATGGTTGATTACTGCGCCTTTGGCTGGATCTTGGCCGCCGTCCTCGGCCTTGCAATTGCACTCTCTTTCTTCGTCTCTCCTAGGAGGAATCGTCGAGGAGGAGCCGATTCCACGCCGAGAAGCGAGGGTGTCAGAAGCTCTTCAACTACCAACGGAGAATGCAGATCCGTCGACGGCGACGCCGACGTTATCATCGTCGGAGCAGGTGTTGCCGGTTCCGCTCTTGCTCACACTCTCGGCAAGGATGGTCGTCTAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGAATCGTCGGTGAATTATTGCAGCCTGGGGGCTACCTCAAATTGATTGAGTTAGGACTGCAAGATTGTGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCCCTTTTCAAGGATGGAAAGAACACTCAACTTTCTTACCCATTGGAAAAATTTCAGTCTGATGTATCTGGAAGAAGCTTCCACAATGGACGCTTCATACAGAGAATGAGGGAGAAGGCTGCTTCCCTTCCCAATGTAAGATTGGAGCAAGGAACAGTTACTTCGTTGCTTGAAGAAAAGGGAACGATCAAAGGTGTGCAGTATAAGTCTAAAAATGGTGAAGAAAAAACAGCGTATGCACCTCTGACAATTGTTTGTGATGGGTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAAGCCTATGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCATGGACATGTTGTTCTCGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGTAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGATTCCTTCTATATCAAATGGTGAAATGGAAAAGTATTTGAAGACCATAGTAGCTCCTCAGGTTCCTCCGCAAATCCATGATGCCTTTATAGCTGCTATTGACAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCTGCTCCCCAACCGACGCCCGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCCCTCACCGGTGGAGGAATGACAGTTGCTTTGTCTGATATTGTTGTGCTCAGGAACCTTCTCAAGCCACTGAAGGACTTGAATGATGCACCAACTCTCTGCAAGTATCTAGAATCCTTTTACACTCTGCGAAAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTCTTTTGTGCATCGCCTGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCCGTGTCCTTGCTCTCGGGATTGAATCCTCGCCCATTAAGTCTGGTTCTCCATTTCTTTGCAGTGGCAATATACGGAGTTGGTCGCTTACTGCTTCCATTTCCTTCTCCAAAAGGCATCTGGATTGGAGCAAGATTGGTCTATAGCGCATCAGGAATCATATTTCCGATTATCAAGGCGGAAGGAGTTAGACAGATGTTCTTCCCTGCTACTGTTCCTGCTTATTACAGAAGCCCACCAGTTCATAAAAGCATAGCATGAAGTGATGGTGATACACAAAGGATCGAACTGGGCATTTGTTTGTATCTACCTGAGGAGGAAGAAGAGGCATCCTCAGGCTGTCGGGCATTGCAGGGAAGACAAATCACTTTTACGTTTGTTAGATTTTCATTTTAATTGTGGATTAATAAACCTGTCTATTTTTGAACAGGAGCCAGGAATATGTAAATGTTCTTAACTCGTTCTAGAAACCCCAACATTAATGTATTTTCAAACAACTTCGGAACTTTCAACATAGTAATACCTAAAACAATCAATTTAGTACTCACCATTACCTTGT

Coding sequence (CDS)

ATGGTTGATTACTGCGCCTTTGGCTGGATCTTGGCCGCCGTCCTCGGCCTTGCAATTGCACTCTCTTTCTTCGTCTCTCCTAGGAGGAATCGTCGAGGAGGAGCCGATTCCACGCCGAGAAGCGAGGGTGTCAGAAGCTCTTCAACTACCAACGGAGAATGCAGATCCGTCGACGGCGACGCCGACGTTATCATCGTCGGAGCAGGTGTTGCCGGTTCCGCTCTTGCTCACACTCTCGGCAAGGATGGTCGTCTAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGAATCGTCGGTGAATTATTGCAGCCTGGGGGCTACCTCAAATTGATTGAGTTAGGACTGCAAGATTGTGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCCCTTTTCAAGGATGGAAAGAACACTCAACTTTCTTACCCATTGGAAAAATTTCAGTCTGATGTATCTGGAAGAAGCTTCCACAATGGACGCTTCATACAGAGAATGAGGGAGAAGGCTGCTTCCCTTCCCAATGTAAGATTGGAGCAAGGAACAGTTACTTCGTTGCTTGAAGAAAAGGGAACGATCAAAGGTGTGCAGTATAAGTCTAAAAATGGTGAAGAAAAAACAGCGTATGCACCTCTGACAATTGTTTGTGATGGGTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAAGCCTATGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCATGGACATGTTGTTCTCGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGTAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGATTCCTTCTATATCAAATGGTGAAATGGAAAAGTATTTGAAGACCATAGTAGCTCCTCAGGTTCCTCCGCAAATCCATGATGCCTTTATAGCTGCTATTGACAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCTGCTCCCCAACCGACGCCCGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCCCTCACCGGTGGAGGAATGACAGTTGCTTTGTCTGATATTGTTGTGCTCAGGAACCTTCTCAAGCCACTGAAGGACTTGAATGATGCACCAACTCTCTGCAAGTATCTAGAATCCTTTTACACTCTGCGAAAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTCTTTTGTGCATCGCCTGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCCGTGTCCTTGCTCTCGGGATTGAATCCTCGCCCATTAAGTCTGGTTCTCCATTTCTTTGCAGTGGCAATATACGGAGTTGGTCGCTTACTGCTTCCATTTCCTTCTCCAAAAGGCATCTGGATTGGAGCAAGATTGGTCTATAGCGCATCAGGAATCATATTTCCGATTATCAAGGCGGAAGGAGTTAGACAGATGTTCTTCCCTGCTACTGTTCCTGCTTATTACAGAAGCCCACCAGTTCATAAAAGCATAGCATGA

Protein sequence

MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVRSSSTTNGECRSVDGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSIA
Homology
BLAST of CmaCh05G001210 vs. ExPASy Swiss-Prot
Match: O48651 (Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 5.6e-236
Identity = 396/526 (75.29%), Postives = 457/526 (86.88%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGAD-STPRSEGVRSSSTTNGECRSVDG 60
           ++D    GWI A + G  + L+F    +R +    +  T  S G  +         ++ G
Sbjct: 23  LIDQYFLGWIFAFLFGFLLLLNF--KRKREKNNSTEFGTDDSNGYYTP-------ENIAG 82

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
             DVIIVGAGVAGSALA+TL  DGR VHVIERDLTE DRIVGELLQPGGYLKLIELGL+D
Sbjct: 83  STDVIIVGAGVAGSALAYTLANDGRRVHVIERDLTEQDRIVGELLQPGGYLKLIELGLED 142

Query: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180
           CV EIDAQ+V+GYAL+ DGKNT+LSYPLEKF SDV+GRSFHNGRF+QRMREKAASLPNVR
Sbjct: 143 CVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHSDVAGRSFHNGRFVQRMREKAASLPNVR 202

Query: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240
           +EQGTVTSL+E+KG++KGVQYK+K+G+E +A+APLTIVCDGCFSNLRRSLC P V+VPSC
Sbjct: 203 MEQGTVTSLVEKKGSVKGVQYKTKDGQELSAFAPLTIVCDGCFSNLRRSLCNPKVEVPSC 262

Query: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYL 300
           FVGL+LEN  LP  NHGHV+L DPSPILFY ISSTEIRCLVDVPGQK+P ISNGE+  YL
Sbjct: 263 FVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPCISNGELANYL 322

Query: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KT+VAPQVP Q++++FIAA+DKGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLTGGG
Sbjct: 323 KTVVAPQVPKQLYNSFIAAVDKGNIRTMPNRSMPADPHPTPGALLLGDAFNMRHPLTGGG 382

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVALSDIV++R+LL+PL+DL+D+ TLCKYLESFYTLRKPVASTINTLAGALYKVFCASP
Sbjct: 383 MTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 442

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480
           D+AR+EMR ACFDYLSLGGI S GP++LLSGLNPRP+SL LHFFAVAIYGVGRLL+PFPS
Sbjct: 443 DKARQEMRNACFDYLSLGGICSQGPIALLSGLNPRPISLFLHFFAVAIYGVGRLLIPFPS 502

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVH 526
           PK +W+GARL+  ASGIIFPIIK+EG+RQMFFPA VPAYYR+PP+H
Sbjct: 503 PKRMWLGARLILGASGIIFPIIKSEGLRQMFFPAIVPAYYRAPPIH 539

BLAST of CmaCh05G001210 vs. ExPASy Swiss-Prot
Match: B7TWW5 (Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2)

HSP 1 Score: 814.3 bits (2102), Expect = 8.0e-235
Identity = 403/530 (76.04%), Postives = 454/530 (85.66%), Query Frame = 0

Query: 10  ILAAVLGLAIALSFFVSPRRNRR----GGADSTPRSEG---VRSSSTTNGECRSVD---G 69
           + AA L     L  F   RR RR    GGA S     G   + SSS  NG C   D    
Sbjct: 16  MFAATLLFGFVLYLFTLRRRRRRREKKGGAGSMEIINGAYKMTSSSEVNGHCTPEDIAGS 75

Query: 70  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 129
             DVIIVGAGVAGSALA+TL KDGR VHVIERDLTE DRIVGELLQPGGYLKL+ELGL+D
Sbjct: 76  SDDVIIVGAGVAGSALAYTLAKDGRRVHVIERDLTEQDRIVGELLQPGGYLKLVELGLED 135

Query: 130 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 189
           CV EIDAQ+V+GYAL+ DGKNT+LSYPLEKF +DV+GRSFHNGRFIQRMREKAASLPNVR
Sbjct: 136 CVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHADVAGRSFHNGRFIQRMREKAASLPNVR 195

Query: 190 LEQGTVTSLLEEKGTIKGVQYKSKNGEE-KTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 249
           +EQGTVTSL+E+KGT+KGV+YK+KNG+E   AYAPLTIVCDGCFSNLR SLC P VDVPS
Sbjct: 196 MEQGTVTSLVEQKGTVKGVRYKTKNGQEMSAAYAPLTIVCDGCFSNLRHSLCNPKVDVPS 255

Query: 250 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKY 309
           CFVGL+LEN  LP  NHGHV+L DPSPILFY ISSTEIRCLVDVPGQK+PSI+NGE+  Y
Sbjct: 256 CFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPSIANGELAHY 315

Query: 310 LKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 369
           LKT VAPQ+PP+++ +FIAAIDKG I+TMPNRSMPA P  TPGALL+GDAFNMRHPLTGG
Sbjct: 316 LKTSVAPQIPPELYKSFIAAIDKGKIKTMPNRSMPADPHSTPGALLLGDAFNMRHPLTGG 375

Query: 370 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 429
           GMTVALSDIV++R+LL+PL+DL+D+ TLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 376 GMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 435

Query: 430 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 489
           PD+AR+EMR ACFDYLSLGGI S GP++LLSGLNPRP+SL  HFFAVAIYGVGRLL+PFP
Sbjct: 436 PDKARQEMRDACFDYLSLGGICSEGPIALLSGLNPRPMSLFFHFFAVAIYGVGRLLIPFP 495

Query: 490 SPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSI 529
           SP+ +W+GARL+  ASGIIFPIIK+EGVRQMFFPATVPAYYR+PP+ K +
Sbjct: 496 SPRKMWLGARLISGASGIIFPIIKSEGVRQMFFPATVPAYYRAPPITKKM 545

BLAST of CmaCh05G001210 vs. ExPASy Swiss-Prot
Match: Q9SM02 (Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 9.2e-231
Identity = 392/524 (74.81%), Postives = 453/524 (86.45%), Query Frame = 0

Query: 7   FGWIL-----AAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVRSSSTTNGECRSVDGD- 66
           + WIL     + ++       FFV P+RN               S+ T++    ++ GD 
Sbjct: 6   WNWILPLLISSLLISFVAFYGFFVKPKRN------GLRHDRKTVSTVTSDVGSVNITGDT 65

Query: 67  -ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 126
            ADVI+VGAGVAGSALA+TLGKD R VHVIERDL+EPDRIVGELLQPGGYLKL+ELG++D
Sbjct: 66  VADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIED 125

Query: 127 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 186
           CVEEIDAQ+VYGYALFK+GK  +L+YPLEKF  DVSGRSFHNGRFIQRMREKAASLPNV+
Sbjct: 126 CVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNVQ 185

Query: 187 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 246
           LEQGTV SLLEE GTIKGV+YK+K GEE+TA+A LTIVCDGCFSNLRRSLC P V+VPSC
Sbjct: 186 LEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPSC 245

Query: 247 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYL 306
           FVGLVLENC LP+ANHGHVVL DPSPIL YPISSTE+RCLVDVPGQK+PSI+NGEM+ YL
Sbjct: 246 FVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNYL 305

Query: 307 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 366
           KT+VAPQ+P +++D+FIAA+DKGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTGGG
Sbjct: 306 KTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGG 365

Query: 367 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 426
           MTVAL+DIVVLRNLL+PL+DL+D  +LCKYLESFYTLRKPVA+TINTLA ALY+VFC+S 
Sbjct: 366 MTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSSE 425

Query: 427 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 486
           ++AR EMR+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PFPS
Sbjct: 426 NEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPS 485

Query: 487 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 524
           PK IW+GA+L+  ASGIIFPIIKAEGVRQMFFPATVPAYY   P
Sbjct: 486 PKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of CmaCh05G001210 vs. ExPASy Swiss-Prot
Match: Q8VYH2 (Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 6.2e-227
Identity = 384/490 (78.37%), Postives = 439/490 (89.59%), Query Frame = 0

Query: 40  RSEGVRSS-STTNGECRSVDG-DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPD 99
           RS+ +  S +  NG      G D D+IIVGAGVAG+ALAHTLGK+GR VHVIERDLTEPD
Sbjct: 32  RSKTIHGSVNVRNGTLTVKSGTDVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPD 91

Query: 100 RIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGR 159
           RIVGELLQPGGYLKLIELGL+DCV++IDAQ+V GYALFKDGK+T+LSYPL++F SDV+GR
Sbjct: 92  RIVGELLQPGGYLKLIELGLEDCVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGR 151

Query: 160 SFHNGRFIQRMREKAASLPNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIV 219
           SFHNGRF+QRMREKA+ LPNVR+EQGTVTSL+EE G IKGVQYK+K+G+E  ++APLTIV
Sbjct: 152 SFHNGRFVQRMREKASLLPNVRMEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIV 211

Query: 220 CDGCFSNLRRSLCKPMVDVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIR 279
           CDGCFSNLRRSLCKP V+VPS FVGLVLENC+LPF NHGHVVLGDPSPILFYPISS+E+R
Sbjct: 212 CDGCFSNLRRSLCKPKVEVPSNFVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVR 271

Query: 280 CLVDVPGQKIPSISNGEMEKYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQ 339
           CLVDVPG K+PS+++GEM  +LKT+VAPQVPPQI DAFI+A++KGNIRTMPNRSMPA P 
Sbjct: 272 CLVDVPGSKLPSVASGEMAHHLKTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPI 331

Query: 340 PTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLR 399
            TPGALL+GDAFNMRHPLTGGGMTVALSDIV+LR+LL PL DL +  +L KY+ESFYTLR
Sbjct: 332 HTPGALLLGDAFNMRHPLTGGGMTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLR 391

Query: 400 KPVASTINTLAGALYKVFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLS 459
           KPVASTINTLAGALYKVF ASPD AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+S
Sbjct: 392 KPVASTINTLAGALYKVFLASPDDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMS 451

Query: 460 LVLHFFAVAIYGVGRLLLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPA 519
           LVLHFFAVAI+GVGRLL+P PS K +W+GARL+ SASGIIFPIIKAEGVRQMFFP T+PA
Sbjct: 452 LVLHFFAVAIFGVGRLLVPLPSVKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPA 511

Query: 520 YYRSPPVHKS 528
            YR+PP   S
Sbjct: 512 IYRAPPTPSS 521

BLAST of CmaCh05G001210 vs. ExPASy Swiss-Prot
Match: O81000 (Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.1e-223
Identity = 375/467 (80.30%), Postives = 422/467 (90.36%), Query Frame = 0

Query: 57  VDGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELG 116
           VD   DVIIVGAGVAGSALAHTLGK+GR VHVIERD +E DRIVGELLQPGGYLKLIELG
Sbjct: 119 VDSGTDVIIVGAGVAGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELG 178

Query: 117 LQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLP 176
           L+DCV++IDAQ+V GY LFKDGK+T+L+YPLE F SDV+GRSFHNGRF+QRMREKA +L 
Sbjct: 179 LEDCVKKIDAQRVLGYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLS 238

Query: 177 NVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 236
           NVRLEQGTVTSLLEE GTIKGV+Y++K G E  ++APLTIVCDGCFSNLRRSLCKP VDV
Sbjct: 239 NVRLEQGTVTSLLEEHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDV 298

Query: 237 PSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEME 296
           PS FVGLVLENC+LPFANHGHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM 
Sbjct: 299 PSTFVGLVLENCELPFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMA 358

Query: 297 KYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLT 356
           KYLKT VAPQVP ++ +AFI A++KGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLT
Sbjct: 359 KYLKTRVAPQVPTKVREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLT 418

Query: 357 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFC 416
           GGGMTVAL+DIVVLR+LL+P+++LND   L KY+ESFYTLRKPVASTINTLA ALYKVF 
Sbjct: 419 GGGMTVALADIVVLRDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFL 478

Query: 417 ASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLP 476
           AS D+AR EMR+ACFDYLSLGG+FS+GPV+LLSGLNPRPLSLVLHFFAVAIY V RL+LP
Sbjct: 479 ASSDEARTEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLP 538

Query: 477 FPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 524
           FPS +  W+GAR++ SAS IIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 539 FPSIESFWLGARIISSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of CmaCh05G001210 vs. TAIR 10
Match: AT1G58440.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 800.8 bits (2067), Expect = 6.5e-232
Identity = 392/524 (74.81%), Postives = 453/524 (86.45%), Query Frame = 0

Query: 7   FGWIL-----AAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVRSSSTTNGECRSVDGD- 66
           + WIL     + ++       FFV P+RN               S+ T++    ++ GD 
Sbjct: 6   WNWILPLLISSLLISFVAFYGFFVKPKRN------GLRHDRKTVSTVTSDVGSVNITGDT 65

Query: 67  -ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 126
            ADVI+VGAGVAGSALA+TLGKD R VHVIERDL+EPDRIVGELLQPGGYLKL+ELG++D
Sbjct: 66  VADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIED 125

Query: 127 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 186
           CVEEIDAQ+VYGYALFK+GK  +L+YPLEKF  DVSGRSFHNGRFIQRMREKAASLPNV+
Sbjct: 126 CVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNVQ 185

Query: 187 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 246
           LEQGTV SLLEE GTIKGV+YK+K GEE+TA+A LTIVCDGCFSNLRRSLC P V+VPSC
Sbjct: 186 LEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPSC 245

Query: 247 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYL 306
           FVGLVLENC LP+ANHGHVVL DPSPIL YPISSTE+RCLVDVPGQK+PSI+NGEM+ YL
Sbjct: 246 FVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNYL 305

Query: 307 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 366
           KT+VAPQ+P +++D+FIAA+DKGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTGGG
Sbjct: 306 KTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGG 365

Query: 367 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 426
           MTVAL+DIVVLRNLL+PL+DL+D  +LCKYLESFYTLRKPVA+TINTLA ALY+VFC+S 
Sbjct: 366 MTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSSE 425

Query: 427 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 486
           ++AR EMR+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PFPS
Sbjct: 426 NEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPS 485

Query: 487 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 524
           PK IW+GA+L+  ASGIIFPIIKAEGVRQMFFPATVPAYY   P
Sbjct: 486 PKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of CmaCh05G001210 vs. TAIR 10
Match: AT4G37760.1 (squalene epoxidase 3 )

HSP 1 Score: 788.1 bits (2034), Expect = 4.4e-228
Identity = 384/490 (78.37%), Postives = 439/490 (89.59%), Query Frame = 0

Query: 40  RSEGVRSS-STTNGECRSVDG-DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPD 99
           RS+ +  S +  NG      G D D+IIVGAGVAG+ALAHTLGK+GR VHVIERDLTEPD
Sbjct: 32  RSKTIHGSVNVRNGTLTVKSGTDVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPD 91

Query: 100 RIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGR 159
           RIVGELLQPGGYLKLIELGL+DCV++IDAQ+V GYALFKDGK+T+LSYPL++F SDV+GR
Sbjct: 92  RIVGELLQPGGYLKLIELGLEDCVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGR 151

Query: 160 SFHNGRFIQRMREKAASLPNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIV 219
           SFHNGRF+QRMREKA+ LPNVR+EQGTVTSL+EE G IKGVQYK+K+G+E  ++APLTIV
Sbjct: 152 SFHNGRFVQRMREKASLLPNVRMEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIV 211

Query: 220 CDGCFSNLRRSLCKPMVDVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIR 279
           CDGCFSNLRRSLCKP V+VPS FVGLVLENC+LPF NHGHVVLGDPSPILFYPISS+E+R
Sbjct: 212 CDGCFSNLRRSLCKPKVEVPSNFVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVR 271

Query: 280 CLVDVPGQKIPSISNGEMEKYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQ 339
           CLVDVPG K+PS+++GEM  +LKT+VAPQVPPQI DAFI+A++KGNIRTMPNRSMPA P 
Sbjct: 272 CLVDVPGSKLPSVASGEMAHHLKTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPI 331

Query: 340 PTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLR 399
            TPGALL+GDAFNMRHPLTGGGMTVALSDIV+LR+LL PL DL +  +L KY+ESFYTLR
Sbjct: 332 HTPGALLLGDAFNMRHPLTGGGMTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLR 391

Query: 400 KPVASTINTLAGALYKVFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLS 459
           KPVASTINTLAGALYKVF ASPD AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+S
Sbjct: 392 KPVASTINTLAGALYKVFLASPDDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMS 451

Query: 460 LVLHFFAVAIYGVGRLLLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPA 519
           LVLHFFAVAI+GVGRLL+P PS K +W+GARL+ SASGIIFPIIKAEGVRQMFFP T+PA
Sbjct: 452 LVLHFFAVAIFGVGRLLVPLPSVKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPA 511

Query: 520 YYRSPPVHKS 528
            YR+PP   S
Sbjct: 512 IYRAPPTPSS 521

BLAST of CmaCh05G001210 vs. TAIR 10
Match: AT2G22830.1 (squalene epoxidase 2 )

HSP 1 Score: 777.3 bits (2006), Expect = 7.7e-225
Identity = 375/467 (80.30%), Postives = 422/467 (90.36%), Query Frame = 0

Query: 57  VDGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELG 116
           VD   DVIIVGAGVAGSALAHTLGK+GR VHVIERD +E DRIVGELLQPGGYLKLIELG
Sbjct: 119 VDSGTDVIIVGAGVAGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELG 178

Query: 117 LQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLP 176
           L+DCV++IDAQ+V GY LFKDGK+T+L+YPLE F SDV+GRSFHNGRF+QRMREKA +L 
Sbjct: 179 LEDCVKKIDAQRVLGYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLS 238

Query: 177 NVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 236
           NVRLEQGTVTSLLEE GTIKGV+Y++K G E  ++APLTIVCDGCFSNLRRSLCKP VDV
Sbjct: 239 NVRLEQGTVTSLLEEHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDV 298

Query: 237 PSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEME 296
           PS FVGLVLENC+LPFANHGHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM 
Sbjct: 299 PSTFVGLVLENCELPFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMA 358

Query: 297 KYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLT 356
           KYLKT VAPQVP ++ +AFI A++KGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLT
Sbjct: 359 KYLKTRVAPQVPTKVREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLT 418

Query: 357 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFC 416
           GGGMTVAL+DIVVLR+LL+P+++LND   L KY+ESFYTLRKPVASTINTLA ALYKVF 
Sbjct: 419 GGGMTVALADIVVLRDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFL 478

Query: 417 ASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLP 476
           AS D+AR EMR+ACFDYLSLGG+FS+GPV+LLSGLNPRPLSLVLHFFAVAIY V RL+LP
Sbjct: 479 ASSDEARTEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLP 538

Query: 477 FPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 524
           FPS +  W+GAR++ SAS IIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 539 FPSIESFWLGARIISSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of CmaCh05G001210 vs. TAIR 10
Match: AT5G24150.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 506.5 bits (1303), Expect = 2.5e-143
Identity = 251/467 (53.75%), Postives = 328/467 (70.24%), Query Frame = 0

Query: 58  DGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 117
           DG  DVIIVGAGV GSALA+ L KDGR VHVIERDL EP+RI+GE +QPGG L L +LGL
Sbjct: 43  DGATDVIIVGAGVGGSALAYALAKDGRRVHVIERDLREPERIMGEFMQPGGRLMLSKLGL 102

Query: 118 QDCVEEIDAQKVYGYALFKDGKNTQLSYPLE--KFQSDVSGRSFHNGRFIQRMREKAASL 177
           +DC+E IDAQK  G  ++KDGK    S+P++   F  D S RSFHNGRF+QR+R+KA+SL
Sbjct: 103 EDCLEGIDAQKATGMTVYKDGKEAVASFPVDNNNFPFDPSARSFHNGRFVQRLRQKASSL 162

Query: 178 PNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVD 237
           PNVRLE+GTV SL+EEKG IKGV YK+  GEE TA APLT+VCDGC+SNLRRSL     +
Sbjct: 163 PNVRLEEGTVKSLIEEKGVIKGVTYKNSAGEETTALAPLTVVCDGCYSNLRRSLNDNNAE 222

Query: 238 VPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEM 297
           V S  VG + +NCQL       +++  PS  + Y ISST++RC+ +V    IPSISNGEM
Sbjct: 223 VLSYQVGFISKNCQLEEPEKLKLIMSKPSFTMLYQISSTDVRCVFEVLPNNIPSISNGEM 282

Query: 298 EKYLKTIVAPQVPPQIHDAFIAAIDKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRHP 357
             ++K  +APQVP ++   F+  ID+G +I+ MP + M A      G +L+GDAFNMRHP
Sbjct: 283 ATFVKNTIAPQVPLKLRKIFLKGIDEGEHIKAMPTKKMTATLSEKKGVILLGDAFNMRHP 342

Query: 358 LTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKV 417
               GM V LSDI++LR LL+PL +L +A  + + ++SFY +RKP+++T+NTL  A  +V
Sbjct: 343 AIASGMMVLLSDILILRRLLQPLSNLGNAQKISQVIKSFYDIRKPMSATVNTLGNAFSQV 402

Query: 418 FCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLL 477
             AS D+A++ MRQ C+DYLS GG  ++G ++LL G+NPRP+SL+ H  A+ +  +G LL
Sbjct: 403 LVASTDEAKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIGHLL 462

Query: 478 LPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRS 522
            PFPSP  IW   RL   A  ++ P +KAEGV QM FP    AY +S
Sbjct: 463 SPFPSPLRIWHSLRLFGLAMKMLVPHLKAEGVSQMLFPVNAAAYSKS 509

BLAST of CmaCh05G001210 vs. TAIR 10
Match: AT5G24160.1 (squalene monoxygenase 6 )

HSP 1 Score: 495.7 bits (1275), Expect = 4.5e-140
Identity = 244/468 (52.14%), Postives = 328/468 (70.09%), Query Frame = 0

Query: 58  DGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 117
           DG ADVIIVGAGV GSALA+ L KDGR VHVIERD+ EP+R++GE +QPGG L L +LGL
Sbjct: 43  DGAADVIIVGAGVGGSALAYALAKDGRRVHVIERDMREPERMMGEFMQPGGRLMLSKLGL 102

Query: 118 QDCVEEIDAQKVYGYALFKDGKNTQLSYPLE--KFQSDVSGRSFHNGRFIQRMREKAASL 177
           QDC+E+IDAQK  G A++KDGK     +P++   F  + S RSFHNGRF+Q++R KA SL
Sbjct: 103 QDCLEDIDAQKATGLAVYKDGKEADAPFPVDNNNFSYEPSARSFHNGRFVQQLRRKAFSL 162

Query: 178 PNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKP-MV 237
            NVRLE+GTV SLLEEKG +KGV YK+K GEE TA APLT+VCDGC+SNLRRSL      
Sbjct: 163 SNVRLEEGTVKSLLEEKGVVKGVTYKNKEGEETTALAPLTVVCDGCYSNLRRSLNDDNNA 222

Query: 238 DVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGE 297
           ++ S  VG + +NC+L      H++L  PS  + Y ISST++RC  +V  +  PSI+NGE
Sbjct: 223 EIMSYIVGYISKNCRLEEPEKLHLILSKPSFTMVYQISSTDVRCGFEVLPENFPSIANGE 282

Query: 298 MEKYLKTIVAPQVPPQIHDAFIAAIDKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRH 357
           M  ++K  + PQVPP++   F+  ID+G +I+ +P + M +      G +++GDAFNMRH
Sbjct: 283 MSTFMKNTIVPQVPPKLRKIFLKGIDEGAHIKVVPAKRMTSTLSKKKGVIVLGDAFNMRH 342

Query: 358 PLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYK 417
           P+   GM V LSDI++LR LL+PL +L DA  + + + SFY +RKP+++T+NTL  A  +
Sbjct: 343 PVVASGMMVLLSDILILRRLLQPLSNLGDANKVSEVINSFYDIRKPMSATVNTLGNAFSQ 402

Query: 418 VFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRL 477
           V   S D+A++ MRQ  +DYL  GG  ++G ++LL G+NPRPLSLV H  A+ +  +G+L
Sbjct: 403 VLIGSTDEAKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLSSIGQL 462

Query: 478 LLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRS 522
           L PFPSP  IW   +L   A  ++ P +KAEGV QM FPA   AY++S
Sbjct: 463 LSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O486515.6e-23675.29Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1[more]
B7TWW58.0e-23576.04Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2[more]
Q9SM029.2e-23174.81Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1[more]
Q8VYH26.2e-22778.37Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1[more]
O810001.1e-22380.30Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1... [more]
Match NameE-valueIdentityDescription
AT1G58440.16.5e-23274.81FAD/NAD(P)-binding oxidoreductase family protein [more]
AT4G37760.14.4e-22878.37squalene epoxidase 3 [more]
AT2G22830.17.7e-22580.30squalene epoxidase 2 [more]
AT5G24150.12.5e-14353.75FAD/NAD(P)-binding oxidoreductase family protein [more]
AT5G24160.14.5e-14052.14squalene monoxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00420RNGMNOXGNASEcoord: 62..84
score: 43.04
coord: 354..370
score: 32.55
coord: 339..354
score: 30.83
coord: 211..226
score: 31.25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..54
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..54
NoneNo IPR availablePANTHERPTHR10835:SF17SQUALENE MONOOXYGENASE-RELATEDcoord: 1..525
IPR013698Squalene epoxidasePFAMPF08491SEcoord: 212..484
e-value: 2.1E-120
score: 400.9
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 50..415
e-value: 1.7E-33
score: 118.0
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 61..436
IPR040125Squalene monooxygenasePANTHERPTHR10835SQUALENE MONOOXYGENASEcoord: 1..525

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G001210.1CmaCh05G001210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0004506 squalene monooxygenase activity