CmaCh05G001030 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G001030
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionFerrochelatase
LocationCma_Chr05: 438953 .. 442395 (-)
RNA-Seq ExpressionCmaCh05G001030
SyntenyCmaCh05G001030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAAGAGTTTCCAGATCCTTCCGTCCCGTTCCTCCTCGTTCGGATTCAGCGTTTCCAATTCTCAAATATTTTTATTCAAGATCGTCTCTGTGAATCCACTCTTTCTGTCATTTCTACATTCGACACTGCTCAAGTTCTTCTTGGATTTTGGATTTGGATACAACTTTTTTCCAATTTTTGACTCCATGGACGCGACCTCTTCCTCTGGCGCTCTTTCTAATGTGAAGCTTCTCAGTTCCAATAAACTCAATTCGGATCACAGGGTTCCCACGTAAGTTGTCTAATGTATCACTTGTGCCTTCCTTCAGCCTTTGCTTGTTGTTTGCGAATGATGATCGGATTCTTAAGTCGCCTTTTGTTGTTACTTCGGAAATTTTCGTTTTATATTATCTGTAGCGTCTGCAAATTTCCGCTCCATATGAACTTTATGTTGATTATTGGATGAAATTACCATATGTTGCAATGTTCGTGCAGCCTGAAACTAGGCATATGAATTTAATTCTGCAAATTTGGAACCAGTCCCCTTAGCTCGATCACTGTGAACTTGTGTGAATGATAATAACAGGGTTGACCAGAAGGAACGTTCTTTGAATTCAAGATGCCACGGCAATGGCATATCTTTTTATACAAGTTCTCAGGTATTTTATTATAGAAGTTAAGAATTCAACATTTGTTTTTCCTTTTTCAGGCTGTTCTCCTTGCCGAAACCTCTCGTGTCTTTATCTTGCAAATCCTATAGAAATTTGCAAGTTCGTGATAAGTCTACATCTCAAGGATTAGTGGTTTCTTGTTCTAGCTCCATTAGTCACAGAGATGTGATGCAGGGGTTACACCTTTCTGGCCCCATTGAGAAGAGAAGTCGTCTTGGACAAGCATTTTGCTCGGTAGGAAGTTTCACCTGTGGTGAATATGCTTTAGAATCTCGCTCTCAGGCAGCGGAAGAGAAGGTTGGAGTTCTGCTTCTGAATCTAGGAGGGCCAGACACGCTTGATGATGTTCAGCCATTTCTGTACAATCTATTTGCAGATCCGGTAGCTTAGTTAATATTATGTATCTAGAGCCTTAATAACATATTAACGTGTCAACATATATCGGTTCTCACTTATTTGCATTAAACATAGGATATTATCCGGCTCCCAAGGTTGTTCCGTTTCCTCCAAGAGCCATTGGCCAAATTAATATCTACCTTTCGTGCTCCCAAAAGCAAAGAAGGGTATGCTTCAATTGGAGGTGGCTCACCTTTGCGTAAAATAACAGATGAGCAGGTTGATTGACCTCTCTCTTGTTCCCCTCTACTTGTTCATTATGTCATATCTCCAGTTGGGTATAAGAAAATCTTCAAATATTTAAATTTGTGTATACTGGATGCAGGCACAGGCGCTTAAAATGGCTTTGGAAGAGAAAAACACATCTGCTAATGTCTATGTGGGAATGCGCTATTGGTACCCTTTCACAGAGGAAGCAATTCAGCAAGTACGTTCTACATTTTTTCTTATTTCCCTTGCCTTATAATTCAAATGCGGGGGGGAATTTTATATTTACTACAGACCACGTGTTATTTCTCAATGTGCCTGTATCTTCAGACTTTAGCATCGTATTTTAGAGATGTTTGAAGTCCTTAATAGTCACTATATTTAATTTAAATATTGGTGGTTCCTTTCGCTGTTTGTGGCAGATTAAGAGGGATGGCATAACAAGACTTGTGGTGCTGCCATTATATCCACAGTACTCTATTTCCACAACTGGGTCTAGTATTCGTGTTCTCCAGAAGATTTTCAGGTGATGACTCAGTCCAGTATTATAATATTTTGATTTTATTTTTTTAAAATAAAAGAGGGATAAAATTGCTTCCAACTTGTTGACTATGACTGTCTATCAGGGAAGATGCATATCTCTCAAAATTGCCTGTTTCAGTTATAAAGTCCTGGTATCAACGTGAAGGGTATATTAAGTCCATGGCTGACTTGGTTCAGGCAGAGCTGAAGAATTTTATCAATCCTCAAGAGGTCCTTGATCTTTGCTGAAACTGATTTTAGAGAGATACTTTAGTTTATGCAACATGATTGTCCCAGTCAATTATTTTCTTATATATAGCATCATTTGCACCTTAGGTCATGATTTTCTTTAGTGCACATGGGGTTCCCGTCAGTTATGTTGAGAATGCTGGAGATCCATACAAAGATCAAATGGAGGAGTGCATCTACCTAATCATGCAAGAGCTGAAAGCCAGAGGAATTGGGAATGAGCATACTCTTGCTTATCAGGTTAAAAATAAATTTTGAGCTTTGCTTGGCTTCTTATCTTTTTCTTGCTTTCAACTATTCAGATGTTCTTTATTTCTTTGTTCCTAAGTACACGCATGTGGAATTTCATTCTTGGTTATGTATTTCATGTTCAGAGCCGAGTTGGACCTGTACAATGGCTGAAGCCATACACAGATGAAGTTCTTGTTGAGCTTGGCCAGAAGGGTATTAAGAGTCTCCTAGCCGTTCCTGTGAGGTATGGTTTGTTCATAAACTATAAATGGATGAACTGTTATCGATTGTTGATTTTGATTCCATGTGTATGTTCATAATTCTAGGAGGTCCTATTATTGCGTATAATTTAAAATATTCATAATCTTGGTAATCAGATCTGACCTTAGAGTGAATGTCAGTTGGACTAAATCATATAACAAGTATATATATTCGTGCGAGCCTAACAATGCAGAAGCTTTGGCCCAATAAGCATTATGCTGTTTGAACGTATTTACAATTGGTTTTACTTGTTTCGAAGACGTGGAGAAGCAAATGTTCAGCGAAGTTCGATTTTTCTCGCATTCCTATGTTGTTTTTTTTATTCTTCTGCCTTTCTGGCGTTTTATTTATAATAATTTTACGTCTCTTCCCAGCTTTGTGAGCGAGCACATTGAGACTCTTGAGGAGATAGATATGGAGTATAAGCACCTGGCACTTGAATCTGGGATCGAAAACTGGGGTCGTGTGCCTGCTCTCAATTGCAAATCTTCTTTCATTTTGGATTTGGCTGATGCAGTGATCGAAGCCCTTCCATCCGCTACAGCTTTGTCGCCCCACGCAAGCTCAACAGATGCAGATGATTGTGATCCTTTCCAATATGCTATCAAATTGCTATTTGGTTCAGTCCTCGCAATAATCTTGTTACTTTCACCAAAAGCATTTCTGGCTTTTAGGAATAATTTCATTTATAGACCATAGCCATCTCAGATATCGCTAGGATTGGGTGAAATGTCATATTTTTTGGGTTAAGGGGTTTGATTTAATTACTTTTGGGAAAAAGAATAGAAATTTACCATCATCGATATATTACCTTTTGTATAACTTCTCTATTTTAGGGAAAATACTGCTGTATTTACCACACAATATATCACATTCATAAAAATGGAGGATTCAGCGTTTTAGAAAACTGTTTATGTAACACCCGAC

mRNA sequence

TCAAGAGTTTCCAGATCCTTCCGTCCCGTTCCTCCTCGTTCGGATTCAGCGTTTCCAATTCTCAAATATTTTTATTCAAGATCGTCTCTGTGAATCCACTCTTTCTGTCATTTCTACATTCGACACTGCTCAAGTTCTTCTTGGATTTTGGATTTGGATACAACTTTTTTCCAATTTTTGACTCCATGGACGCGACCTCTTCCTCTGGCGCTCTTTCTAATGTGAAGCTTCTCAGTTCCAATAAACTCAATTCGGATCACAGGGTTCCCACGCTGTTCTCCTTGCCGAAACCTCTCGTGTCTTTATCTTGCAAATCCTATAGAAATTTGCAAGTTCGTGATAAGTCTACATCTCAAGGATTAGTGGTTTCTTGTTCTAGCTCCATTAGTCACAGAGATGTGATGCAGGGGTTACACCTTTCTGGCCCCATTGAGAAGAGAAGTCGTCTTGGACAAGCATTTTGCTCGGTAGGAAGTTTCACCTGTGGTGAATATGCTTTAGAATCTCGCTCTCAGGCAGCGGAAGAGAAGGTTGGAGTTCTGCTTCTGAATCTAGGAGGGCCAGACACGCTTGATGATGTTCAGCCATTTCTGTACAATCTATTTGCAGATCCGGATATTATCCGGCTCCCAAGGTTGTTCCGTTTCCTCCAAGAGCCATTGGCCAAATTAATATCTACCTTTCGTGCTCCCAAAAGCAAAGAAGGGTATGCTTCAATTGGAGGTGGCTCACCTTTGCGTAAAATAACAGATGAGCAGGCACAGGCGCTTAAAATGGCTTTGGAAGAGAAAAACACATCTGCTAATGTCTATGTGGGAATGCGCTATTGGTACCCTTTCACAGAGGAAGCAATTCAGCAAATTAAGAGGGATGGCATAACAAGACTTGTGGTGCTGCCATTATATCCACAGTACTCTATTTCCACAACTGGGTCTAGTATTCGTGTTCTCCAGAAGATTTTCAGGGAAGATGCATATCTCTCAAAATTGCCTGTTTCAGTTATAAAGTCCTGGTATCAACGTGAAGGGTATATTAAGTCCATGGCTGACTTGGTTCAGGCAGAGCTGAAGAATTTTATCAATCCTCAAGAGGTCATGATTTTCTTTAGTGCACATGGGGTTCCCGTCAGTTATGTTGAGAATGCTGGAGATCCATACAAAGATCAAATGGAGGAGTGCATCTACCTAATCATGCAAGAGCTGAAAGCCAGAGGAATTGGGAATGAGCATACTCTTGCTTATCAGAGCCGAGTTGGACCTGTACAATGGCTGAAGCCATACACAGATGAAGTTCTTGTTGAGCTTGGCCAGAAGGGTATTAAGAGTCTCCTAGCCGTTCCTGTGAGCTTTGTGAGCGAGCACATTGAGACTCTTGAGGAGATAGATATGGAGTATAAGCACCTGGCACTTGAATCTGGGATCGAAAACTGGGGTCGTGTGCCTGCTCTCAATTGCAAATCTTCTTTCATTTTGGATTTGGCTGATGCAGTGATCGAAGCCCTTCCATCCGCTACAGCTTTGTCGCCCCACGCAAGCTCAACAGATGCAGATGATTGTGATCCTTTCCAATATGCTATCAAATTGCTATTTGGTTCAGTCCTCGCAATAATCTTGTTACTTTCACCAAAAGCATTTCTGGCTTTTAGGAATAATTTCATTTATAGACCATAGCCATCTCAGATATCGCTAGGATTGGGTGAAATGTCATATTTTTTGGGTTAAGGGGTTTGATTTAATTACTTTTGGGAAAAAGAATAGAAATTTACCATCATCGATATATTACCTTTTGTATAACTTCTCTATTTTAGGGAAAATACTGCTGTATTTACCACACAATATATCACATTCATAAAAATGGAGGATTCAGCGTTTTAGAAAACTGTTTATGTAACACCCGAC

Coding sequence (CDS)

ATGGACGCGACCTCTTCCTCTGGCGCTCTTTCTAATGTGAAGCTTCTCAGTTCCAATAAACTCAATTCGGATCACAGGGTTCCCACGCTGTTCTCCTTGCCGAAACCTCTCGTGTCTTTATCTTGCAAATCCTATAGAAATTTGCAAGTTCGTGATAAGTCTACATCTCAAGGATTAGTGGTTTCTTGTTCTAGCTCCATTAGTCACAGAGATGTGATGCAGGGGTTACACCTTTCTGGCCCCATTGAGAAGAGAAGTCGTCTTGGACAAGCATTTTGCTCGGTAGGAAGTTTCACCTGTGGTGAATATGCTTTAGAATCTCGCTCTCAGGCAGCGGAAGAGAAGGTTGGAGTTCTGCTTCTGAATCTAGGAGGGCCAGACACGCTTGATGATGTTCAGCCATTTCTGTACAATCTATTTGCAGATCCGGATATTATCCGGCTCCCAAGGTTGTTCCGTTTCCTCCAAGAGCCATTGGCCAAATTAATATCTACCTTTCGTGCTCCCAAAAGCAAAGAAGGGTATGCTTCAATTGGAGGTGGCTCACCTTTGCGTAAAATAACAGATGAGCAGGCACAGGCGCTTAAAATGGCTTTGGAAGAGAAAAACACATCTGCTAATGTCTATGTGGGAATGCGCTATTGGTACCCTTTCACAGAGGAAGCAATTCAGCAAATTAAGAGGGATGGCATAACAAGACTTGTGGTGCTGCCATTATATCCACAGTACTCTATTTCCACAACTGGGTCTAGTATTCGTGTTCTCCAGAAGATTTTCAGGGAAGATGCATATCTCTCAAAATTGCCTGTTTCAGTTATAAAGTCCTGGTATCAACGTGAAGGGTATATTAAGTCCATGGCTGACTTGGTTCAGGCAGAGCTGAAGAATTTTATCAATCCTCAAGAGGTCATGATTTTCTTTAGTGCACATGGGGTTCCCGTCAGTTATGTTGAGAATGCTGGAGATCCATACAAAGATCAAATGGAGGAGTGCATCTACCTAATCATGCAAGAGCTGAAAGCCAGAGGAATTGGGAATGAGCATACTCTTGCTTATCAGAGCCGAGTTGGACCTGTACAATGGCTGAAGCCATACACAGATGAAGTTCTTGTTGAGCTTGGCCAGAAGGGTATTAAGAGTCTCCTAGCCGTTCCTGTGAGCTTTGTGAGCGAGCACATTGAGACTCTTGAGGAGATAGATATGGAGTATAAGCACCTGGCACTTGAATCTGGGATCGAAAACTGGGGTCGTGTGCCTGCTCTCAATTGCAAATCTTCTTTCATTTTGGATTTGGCTGATGCAGTGATCGAAGCCCTTCCATCCGCTACAGCTTTGTCGCCCCACGCAAGCTCAACAGATGCAGATGATTGTGATCCTTTCCAATATGCTATCAAATTGCTATTTGGTTCAGTCCTCGCAATAATCTTGTTACTTTCACCAAAAGCATTTCTGGCTTTTAGGAATAATTTCATTTATAGACCATAG

Protein sequence

MDATSSSGALSNVKLLSSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGLVVSCSSSISHRDVMQGLHLSGPIEKRSRLGQAFCSVGSFTCGEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFIYRP
Homology
BLAST of CmaCh05G001030 vs. ExPASy Swiss-Prot
Match: P42044 (Ferrochelatase-2, chloroplastic OS=Cucumis sativus OX=3659 GN=HEMH PE=2 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 9.4e-246
Identity = 435/492 (88.41%), Postives = 458/492 (93.09%), Query Frame = 0

Query: 1   MDATSSSGALSNVKLL-SSNKLNSDHRVPTLFSLPKPLVSLSCKSYRNLQVRDKSTSQGL 60
           MDA SSS ALSN+KL  S+N LNSD R+ +L SLPK  V+ SCK+  NLQVRD+ST  GL
Sbjct: 1   MDAASSSLALSNIKLHGSTNTLNSDQRISSLCSLPKSRVTFSCKTSGNLQVRDRST--GL 60

Query: 61  VVSCSSSISHRDVMQGLHLSGPIEKRSRLGQAFCSVGSFTCGEYALESRSQAAEEKVGVL 120
           VVSCSSS   RDV+QGLHLSGPIEK+SRLGQA CSVG+FT GE+ALES+SQA ++KVGVL
Sbjct: 61  VVSCSSSNGDRDVIQGLHLSGPIEKKSRLGQACCSVGTFTVGEFALESQSQAVDDKVGVL 120

Query: 121 LLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIG 180
           LLNLGGP+TLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLIST+RAPKSKEGYASIG
Sbjct: 121 LLNLGGPETLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTYRAPKSKEGYASIG 180

Query: 181 GGSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPL 240
           GGSPLRKITDEQAQALKMAL EKN S NVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPL
Sbjct: 181 GGSPLRKITDEQAQALKMALAEKNMSTNVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPL 240

Query: 241 YPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFIN 300
           YPQYSISTTGSSIRVLQK+FREDAYLS LPVS+IKSWYQREGYIKSMADL+QAELKNF N
Sbjct: 241 YPQYSISTTGSSIRVLQKMFREDAYLSSLPVSIIKSWYQREGYIKSMADLMQAELKNFAN 300

Query: 301 PQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPV 360
           PQEVMIFFSAHGVPVSYVENAGDPYKDQMEECI LIMQELKARGIGNEHTLAYQSRVGPV
Sbjct: 301 PQEVMIFFSAHGVPVSYVENAGDPYKDQMEECICLIMQELKARGIGNEHTLAYQSRVGPV 360

Query: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVP 420
           QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGI+NWGRVP
Sbjct: 361 QWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIQNWGRVP 420

Query: 421 ALNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLS 480
           ALNC SSFI DLADAVIEALPSATAL+PH SSTDADD DPF YAIKLLFGSVLA ILLLS
Sbjct: 421 ALNCNSSFISDLADAVIEALPSATALAPHTSSTDADDHDPFLYAIKLLFGSVLAFILLLS 480

Query: 481 PKAFLAFRNNFI 492
           PKAF+ FRNNF+
Sbjct: 481 PKAFMVFRNNFL 490

BLAST of CmaCh05G001030 vs. ExPASy Swiss-Prot
Match: Q69TB1 (Ferrochelatase-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0297000 PE=2 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 5.1e-175
Identity = 308/417 (73.86%), Postives = 349/417 (83.69%), Query Frame = 0

Query: 76  LHLSGPIEKRSRLGQAFCSVGSFTC-GEYALESRSQAAEEKVGVLLLNLGGPDTLDDVQP 135
           +H S  +++R  L     S   +T   E      S AAEEKVGVLLLNLGGP+TLDDVQP
Sbjct: 66  VHRSPVLKQRQNLSARSTSADVYTTFDENVRAVSSHAAEEKVGVLLLNLGGPETLDDVQP 125

Query: 136 FLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQA 195
           FL+NLFADPDIIRLPRLFRFLQ PLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQA A
Sbjct: 126 FLFNLFADPDIIRLPRLFRFLQRPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQANA 185

Query: 196 LKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRV 255
           LK+AL++KN +AN+YVGMRYWYPFTEEAI QIK+D IT+LVVLPLYPQYSIST+GSSIRV
Sbjct: 186 LKVALKKKNLNANIYVGMRYWYPFTEEAIDQIKKDKITKLVVLPLYPQYSISTSGSSIRV 245

Query: 256 LQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPV 315
           LQ I +ED+Y + LP+S+I+SWYQR+GY+KSMADL++ EL  F NP+EVMIFFSAHGVP+
Sbjct: 246 LQNIVKEDSYFAGLPISIIESWYQRDGYVKSMADLIEKELSIFSNPEEVMIFFSAHGVPL 305

Query: 316 SYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELG 375
           +YV +AGDPY+DQME+CI LIM ELK+RGI N HTLAYQSRVGPVQWLKPYTDEVLVELG
Sbjct: 306 TYVTDAGDPYRDQMEDCIALIMGELKSRGILNSHTLAYQSRVGPVQWLKPYTDEVLVELG 365

Query: 376 QKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADA 435
           Q+G+KSLLAVPVSFVSEHIETLEEIDMEYK LALESGIENWGRVPAL C SSFI DLADA
Sbjct: 366 QQGVKSLLAVPVSFVSEHIETLEEIDMEYKELALESGIENWGRVPALGCTSSFISDLADA 425

Query: 436 VIEALPSATALSPHASSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRNNFI 492
           V+EALPSA+AL          D D   Y  K+ FGS+LA +LLLSP+   AFRN  +
Sbjct: 426 VVEALPSASALVTKKVDESDSDMDLMHYLSKMFFGSILAFVLLLSPRLISAFRNTLL 482

BLAST of CmaCh05G001030 vs. ExPASy Swiss-Prot
Match: P42045 (Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 3.4e-171
Identity = 296/380 (77.89%), Postives = 332/380 (87.37%), Query Frame = 0

Query: 109 SQAAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRA 168
           S A EEKVGVLLLNLGGP+TL+DVQPFL+NLFADPDIIRLPRLFRFLQ PLAKLISTFRA
Sbjct: 102 SHAVEEKVGVLLLNLGGPETLNDVQPFLFNLFADPDIIRLPRLFRFLQRPLAKLISTFRA 161

Query: 169 PKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKR 228
           PKS EGYASIGGGSPLRKITDEQA ALK+AL+ KN  A++YVGMRYWYPFTEEAI QIK+
Sbjct: 162 PKSNEGYASIGGGSPLRKITDEQANALKVALKSKNLEADIYVGMRYWYPFTEEAIDQIKK 221

Query: 229 DGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMAD 288
           D IT+LVVLPLYPQYSIST+GSSIRVLQ I +ED Y + LP+S+I+SWYQREGY+KSMAD
Sbjct: 222 DKITKLVVLPLYPQYSISTSGSSIRVLQNIVKEDPYFAGLPISIIESWYQREGYVKSMAD 281

Query: 289 LVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEH 348
           L++ EL  F NP+EVMIFFSAHGVP++YV++AGDPY+DQME+CI LIM+ELK+RG  N+H
Sbjct: 282 LIEKELSVFSNPEEVMIFFSAHGVPLTYVKDAGDPYRDQMEDCIALIMEELKSRGTLNDH 341

Query: 349 TLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLAL 408
           TLAYQSRVGPVQWLKPYTDEVLVELGQKG+KSLLAVPVSFVSEHIETLEEIDMEY+ LAL
Sbjct: 342 TLAYQSRVGPVQWLKPYTDEVLVELGQKGVKSLLAVPVSFVSEHIETLEEIDMEYRELAL 401

Query: 409 ESGIENWGRVPALNCKSSFILDLADAVIEALPSATALSPHASSTDADDCDPFQYAIKLLF 468
           ESGIENWGRVPAL C SSFI DLADAV+EALPSA+A++         D D   Y  K+  
Sbjct: 402 ESGIENWGRVPALGCTSSFISDLADAVVEALPSASAMATRKVKDTDSDMDMMHYLTKMFL 461

Query: 469 GSVLAIILLLSPKAFLAFRN 489
           GSVLA  LLLSP+   AFRN
Sbjct: 462 GSVLAFFLLLSPRLVSAFRN 481

BLAST of CmaCh05G001030 vs. ExPASy Swiss-Prot
Match: P42043 (Ferrochelatase-1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FC1 PE=2 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 6.5e-170
Identity = 299/403 (74.19%), Postives = 346/403 (85.86%), Query Frame = 0

Query: 90  QAFCSVGSFTCGEYALESRSQ-AAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRL 149
           QA    G  +  E + ++RS   AE+K+GVLLLNLGGP+TL+DVQPFLYNLFADPDIIRL
Sbjct: 63  QARSVTGDCSYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRL 122

Query: 150 PRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSANV 209
           PR F+FLQ  +AK IS  RAPKSKEGYA+IGGGSPLRKITDEQA A+KM+L+ KN +ANV
Sbjct: 123 PRPFQFLQGTIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANV 182

Query: 210 YVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKL 269
           YVGMRYWYPFTEEA+QQIK+D ITRLVVLPLYPQYSISTTGSSIRVLQ +FR+D YL+ +
Sbjct: 183 YVGMRYWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGV 242

Query: 270 PVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQM 329
           PV++IKSWYQR GY+ SMADL++ EL+ F +P+EVMIFFSAHGVPVSYVENAGDPY+ QM
Sbjct: 243 PVAIIKSWYQRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQM 302

Query: 330 EECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSF 389
           EECI LIM+ELKARG+ N+H LAYQSRVGPVQWLKPYTDEVLV+LG+ G+KSLLAVPVSF
Sbjct: 303 EECIDLIMEELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSF 362

Query: 390 VSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS-P 449
           VSEHIETLEEIDMEY+ LALESG+ENWGRVPAL    SFI DLADAVIE+LPSA A+S P
Sbjct: 363 VSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNP 422

Query: 450 HA--SSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRN 489
           +A   S D++  D F Y +K+ FGS+LA +LLLSPK F AFRN
Sbjct: 423 NAVVDSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465

BLAST of CmaCh05G001030 vs. ExPASy Swiss-Prot
Match: Q0DIV0 (Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0361200 PE=2 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.2e-147
Identity = 252/333 (75.68%), Postives = 297/333 (89.19%), Query Frame = 0

Query: 114 EKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKLISTFRAPKSKE 173
           EK+GVLLLNLGGP+TLDDVQPFL+NLFADPDIIRLPRLFRFLQ+PLA+ IS  RAPKSKE
Sbjct: 112 EKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQKPLAQFISVVRAPKSKE 171

Query: 174 GYASIGGGSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEAIQQIKRDGITR 233
           GYASIGGGSPLR+ITD QA+AL+ AL +K+  A VYVGMRYW+PFTEEAI+QIKRDGIT+
Sbjct: 172 GYASIGGGSPLRQITDAQAEALRKALCDKDIPAKVYVGMRYWHPFTEEAIEQIKRDGITK 231

Query: 234 LVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGYIKSMADLVQAE 293
           LVVLPLYPQ+SIST+GSS+R+L+ IFRED YL  +  +VI SWYQREGYIK+MA L++ E
Sbjct: 232 LVVLPLYPQFSISTSGSSLRLLEGIFREDEYLVNMQHTVIPSWYQREGYIKAMATLIEKE 291

Query: 294 LKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKARGIGNEHTLAYQ 353
           L+ F  PQ+VMIFFSAHGVP++YVE AGDPYK +MEEC+ LIM+EL+ RGI N  TLAYQ
Sbjct: 292 LRTFSEPQKVMIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELEKRGITNSCTLAYQ 351

Query: 354 SRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDMEYKHLALESGIE 413
           SRVGPV+WL+PYTDE ++ELGQKG+KSLLAVP+SFVSEHIETLEEID+EYK LALESGI+
Sbjct: 352 SRVGPVEWLRPYTDETIIELGQKGVKSLLAVPISFVSEHIETLEEIDVEYKELALESGIK 411

Query: 414 NWGRVPALNCKSSFILDLADAVIEALPSATALS 447
           +WGRVPAL C+ +FI DLADAVIE+LP   A++
Sbjct: 412 HWGRVPALGCEPTFITDLADAVIESLPYVGAMA 444

BLAST of CmaCh05G001030 vs. TAIR 10
Match: AT5G26030.1 (ferrochelatase 1 )

HSP 1 Score: 598.6 bits (1542), Expect = 4.6e-171
Identity = 299/403 (74.19%), Postives = 346/403 (85.86%), Query Frame = 0

Query: 90  QAFCSVGSFTCGEYALESRSQ-AAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRL 149
           QA    G  +  E + ++RS   AE+K+GVLLLNLGGP+TL+DVQPFLYNLFADPDIIRL
Sbjct: 63  QARSVTGDCSYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRL 122

Query: 150 PRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSANV 209
           PR F+FLQ  +AK IS  RAPKSKEGYA+IGGGSPLRKITDEQA A+KM+L+ KN +ANV
Sbjct: 123 PRPFQFLQGTIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANV 182

Query: 210 YVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKL 269
           YVGMRYWYPFTEEA+QQIK+D ITRLVVLPLYPQYSISTTGSSIRVLQ +FR+D YL+ +
Sbjct: 183 YVGMRYWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGV 242

Query: 270 PVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQM 329
           PV++IKSWYQR GY+ SMADL++ EL+ F +P+EVMIFFSAHGVPVSYVENAGDPY+ QM
Sbjct: 243 PVAIIKSWYQRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQM 302

Query: 330 EECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSF 389
           EECI LIM+ELKARG+ N+H LAYQSRVGPVQWLKPYTDEVLV+LG+ G+KSLLAVPVSF
Sbjct: 303 EECIDLIMEELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSF 362

Query: 390 VSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS-P 449
           VSEHIETLEEIDMEY+ LALESG+ENWGRVPAL    SFI DLADAVIE+LPSA A+S P
Sbjct: 363 VSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNP 422

Query: 450 HA--SSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRN 489
           +A   S D++  D F Y +K+ FGS+LA +LLLSPK F AFRN
Sbjct: 423 NAVVDSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465

BLAST of CmaCh05G001030 vs. TAIR 10
Match: AT5G26030.2 (ferrochelatase 1 )

HSP 1 Score: 598.6 bits (1542), Expect = 4.6e-171
Identity = 299/403 (74.19%), Postives = 346/403 (85.86%), Query Frame = 0

Query: 90  QAFCSVGSFTCGEYALESRSQ-AAEEKVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRL 149
           QA    G  +  E + ++RS   AE+K+GVLLLNLGGP+TL+DVQPFLYNLFADPDIIRL
Sbjct: 63  QARSVTGDCSYDETSAKARSHVVAEDKIGVLLLNLGGPETLNDVQPFLYNLFADPDIIRL 122

Query: 150 PRLFRFLQEPLAKLISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSANV 209
           PR F+FLQ  +AK IS  RAPKSKEGYA+IGGGSPLRKITDEQA A+KM+L+ KN +ANV
Sbjct: 123 PRPFQFLQGTIAKFISVVRAPKSKEGYAAIGGGSPLRKITDEQADAIKMSLQAKNIAANV 182

Query: 210 YVGMRYWYPFTEEAIQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKL 269
           YVGMRYWYPFTEEA+QQIK+D ITRLVVLPLYPQYSISTTGSSIRVLQ +FR+D YL+ +
Sbjct: 183 YVGMRYWYPFTEEAVQQIKKDKITRLVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGV 242

Query: 270 PVSVIKSWYQREGYIKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQM 329
           PV++IKSWYQR GY+ SMADL++ EL+ F +P+EVMIFFSAHGVPVSYVENAGDPY+ QM
Sbjct: 243 PVAIIKSWYQRRGYVNSMADLIEKELQTFSDPKEVMIFFSAHGVPVSYVENAGDPYQKQM 302

Query: 330 EECIYLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSF 389
           EECI LIM+ELKARG+ N+H LAYQSRVGPVQWLKPYTDEVLV+LG+ G+KSLLAVPVSF
Sbjct: 303 EECIDLIMEELKARGVLNDHKLAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSF 362

Query: 390 VSEHIETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS-P 449
           VSEHIETLEEIDMEY+ LALESG+ENWGRVPAL    SFI DLADAVIE+LPSA A+S P
Sbjct: 363 VSEHIETLEEIDMEYRELALESGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNP 422

Query: 450 HA--SSTDADDCDPFQYAIKLLFGSVLAIILLLSPKAFLAFRN 489
           +A   S D++  D F Y +K+ FGS+LA +LLLSPK F AFRN
Sbjct: 423 NAVVDSEDSESSDAFSYIVKMFFGSILAFVLLLSPKMFHAFRN 465

BLAST of CmaCh05G001030 vs. TAIR 10
Match: AT2G30390.1 (ferrochelatase 2 )

HSP 1 Score: 503.8 bits (1296), Expect = 1.5e-142
Identity = 244/344 (70.93%), Postives = 296/344 (86.05%), Query Frame = 0

Query: 104 ALESRSQAAEE-KVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKL 163
           A+ S S   ++ K+GVLLLNLGGP+TLDDVQPFL+NLFADPDIIRLP +F+FLQ+PLA+ 
Sbjct: 88  AISSSSVITDDAKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPPVFQFLQKPLAQF 147

Query: 164 ISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEA 223
           IS  RAPKSKEGYASIGGGSPLR ITD QA+ L+  L EKN  A VYVGMRYW+PFTEEA
Sbjct: 148 ISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKNVPAKVYVGMRYWHPFTEEA 207

Query: 224 IQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGY 283
           I+QIKRDGIT+LVVLPLYPQ+SIST+GSS+R+L++IFRED YL  +  +VI SWYQREGY
Sbjct: 208 IEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVIPSWYQREGY 267

Query: 284 IKSMADLVQAELKNFINPQEVMIFFSAHGVPVSYVENAGDPYKDQMEECIYLIMQELKAR 343
           IK+MA+L+Q+EL  F +P +V+IFFSAHGVP++YVE AGDPYK +MEEC+ LIM+EL  R
Sbjct: 268 IKAMANLIQSELGKFGSPNQVVIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELDKR 327

Query: 344 GIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEHIETLEEIDME 403
            I N +TLAYQSRVGPV+WLKPYT+E + ELG+KG+++LLAVP+SFVSEHIETLEEID+E
Sbjct: 328 KITNAYTLAYQSRVGPVEWLKPYTEEAITELGKKGVENLLAVPISFVSEHIETLEEIDVE 387

Query: 404 YKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 447
           YK LAL+SGI+NWGRVPAL  +  FI DLADAV+E+LP   A++
Sbjct: 388 YKELALKSGIKNWGRVPALGTEPMFISDLADAVVESLPYVGAMA 431

BLAST of CmaCh05G001030 vs. TAIR 10
Match: AT2G30390.2 (ferrochelatase 2 )

HSP 1 Score: 495.7 bits (1275), Expect = 4.2e-140
Identity = 244/354 (68.93%), Postives = 296/354 (83.62%), Query Frame = 0

Query: 104 ALESRSQAAEE-KVGVLLLNLGGPDTLDDVQPFLYNLFADPDIIRLPRLFRFLQEPLAKL 163
           A+ S S   ++ K+GVLLLNLGGP+TLDDVQPFL+NLFADPDIIRLP +F+FLQ+PLA+ 
Sbjct: 88  AISSSSVITDDAKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPPVFQFLQKPLAQF 147

Query: 164 ISTFRAPKSKEGYASIGGGSPLRKITDEQAQALKMALEEKNTSANVYVGMRYWYPFTEEA 223
           IS  RAPKSKEGYASIGGGSPLR ITD QA+ L+  L EKN  A VYVGMRYW+PFTEEA
Sbjct: 148 ISVARAPKSKEGYASIGGGSPLRHITDAQAEELRKCLWEKNVPAKVYVGMRYWHPFTEEA 207

Query: 224 IQQIKRDGITRLVVLPLYPQYSISTTGSSIRVLQKIFREDAYLSKLPVSVIKSWYQREGY 283
           I+QIKRDGIT+LVVLPLYPQ+SIST+GSS+R+L++IFRED YL  +  +VI SWYQREGY
Sbjct: 208 IEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVIPSWYQREGY 267

Query: 284 IKSMADLVQAELKNFINPQ----------EVMIFFSAHGVPVSYVENAGDPYKDQMEECI 343
           IK+MA+L+Q+EL  F +P           +V+IFFSAHGVP++YVE AGDPYK +MEEC+
Sbjct: 268 IKAMANLIQSELGKFGSPNQTCFKPKYMLQVVIFFSAHGVPLAYVEEAGDPYKAEMEECV 327

Query: 344 YLIMQELKARGIGNEHTLAYQSRVGPVQWLKPYTDEVLVELGQKGIKSLLAVPVSFVSEH 403
            LIM+EL  R I N +TLAYQSRVGPV+WLKPYT+E + ELG+KG+++LLAVP+SFVSEH
Sbjct: 328 DLIMEELDKRKITNAYTLAYQSRVGPVEWLKPYTEEAITELGKKGVENLLAVPISFVSEH 387

Query: 404 IETLEEIDMEYKHLALESGIENWGRVPALNCKSSFILDLADAVIEALPSATALS 447
           IETLEEID+EYK LAL+SGI+NWGRVPAL  +  FI DLADAV+E+LP   A++
Sbjct: 388 IETLEEIDVEYKELALKSGIKNWGRVPALGTEPMFISDLADAVVESLPYVGAMA 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P420449.4e-24688.41Ferrochelatase-2, chloroplastic OS=Cucumis sativus OX=3659 GN=HEMH PE=2 SV=1[more]
Q69TB15.1e-17573.86Ferrochelatase-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os09... [more]
P420453.4e-17177.89Ferrochelatase-2, chloroplastic OS=Hordeum vulgare OX=4513 GN=HEMH PE=2 SV=1[more]
P420436.5e-17074.19Ferrochelatase-1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q0DIV01.2e-14775.68Ferrochelatase-2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05... [more]
Match NameE-valueIdentityDescription
AT5G26030.14.6e-17174.19ferrochelatase 1 [more]
AT5G26030.24.6e-17174.19ferrochelatase 1 [more]
AT2G30390.11.5e-14270.93ferrochelatase 2 [more]
AT2G30390.24.2e-14068.93ferrochelatase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001015FerrochelatasePFAMPF00762Ferrochelatasecoord: 115..438
e-value: 2.0E-111
score: 372.1
IPR001015FerrochelataseTIGRFAMTIGR00109TIGR00109coord: 113..438
e-value: 3.4E-115
score: 382.8
IPR001015FerrochelatasePANTHERPTHR11108FERROCHELATASEcoord: 51..490
IPR001015FerrochelataseHAMAPMF_00323Ferrochelatasecoord: 114..439
score: 37.142395
NoneNo IPR availableGENE3D3.40.50.1400coord: 115..439
e-value: 7.6E-122
score: 408.6
NoneNo IPR availableGENE3D3.40.50.1400coord: 277..420
e-value: 7.6E-122
score: 408.6
NoneNo IPR availablePANTHERPTHR11108:SF4FERROCHELATASE-1, CHLOROPLASTIC/MITOCHONDRIALcoord: 51..490
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 114..446
IPR019772Ferrochelatase, active sitePROSITEPS00534FERROCHELATASEcoord: 305..324
IPR033644Ferrochelatase, C-terminalCDDcd00419Ferrochelatase_Ccoord: 286..422
e-value: 2.70904E-57
score: 184.655
IPR033659Ferrochelatase, N-terminalCDDcd03411Ferrochelatase_Ncoord: 116..278
e-value: 5.14618E-71
score: 221.29

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G001030.1CmaCh05G001030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006783 heme biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004325 ferrochelatase activity