CmaCh05G000150 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G000150
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpolygalacturonase At1g48100-like
LocationCma_Chr05: 77098 .. 80992 (-)
RNA-Seq ExpressionCmaCh05G000150
SyntenyCmaCh05G000150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGAAAAAAATAAATGCCTGCCATTATCAAAATGGTATGTTGTCTAGCATTTGTCTGCACAGTCTCCTGAAGCTTTTACAGAGGCTCCACCTATTTAACCCCGCTTCACCTCCCACTTGCTTTGGCACTCTCAAAACCACTGACTCTCTCTCTGTTTCTCCTCTCATTCGCCACTGAAAATGATGAGTGGGATGAGTTCCAAAAGCTTAACACTTGTAGTTTTTATTGCTTTTCTTGTTTGGTCTTCAAACTTTGAAGCTTGCATTGCAAGGAGAGGAAAACACTGGAGACAGAGCAGGGAGGCCTCTGCTTCTCTATCCAAGAAGAAGGGCAGTCATGGCGGTAGCAAGAGCCACCATTCCGGTGGTTTCCACTCCAAAGCTCCACCGCCACACAATGCCTCTCCTCCCTCTCCAATTCCTCCTAAGCCCAAAGAAGAGATCGTTCAAACTCCACCCAAGAAAGGCTACAATGGCGGCGATTCTGCCATATTCAACGTTCTAGATTTTGGTGCTAAAGGCAATGGAGAGACTGATGACACTAAGGTACGTTCAATTCTACACCATATTCAACCTTCTTTTTCCTCCTATACAAATACTTGGAGAATGACATCTAACAGAATTTAGCCACTGCACAAGGATAAAGTTCCATTAATAATAATATTTGTACAAATGTGAATGATCATTTGTGTTGAAATACCAGCTTAAATCACCCCCCCCCCACCTTCTTAAATCATTGTTTGAAATACCAGCTTAAATCATTTGTGGGAAAAAGAGCAGCTATTATGGCGAAATAAGCTAAACCAAAAGAATTGCCAGAATAATAATAATAACCTAAGAAAAATAAAAGGTAGCTGACACGCAGCTTAATGTAACCCGGCCTTCAGGACCAGCTACCAGCAGGCGGGACCCACAGCTAAGGATAGAAAATAACTTAGTTATGACAGTTTTCTTATTAAAACAAAAAATAAGTTAAATTTTTTTTGCACCATCCGTTTCAGGCATTTGAAGACGCTTGGGCGGCCGCTTGTAAGGTGGAGGGATCGACGGTCATGGTTCCCGCAGAATATGTGTTCTACGTAGGACCCATTTCCTTCTCTGGCCCATATTGCCAACCGAACATTGTATTTCAGGTAAGCATTATCGAACCCAGAAGCTCTTGTTTTACGCTTTTAAGTTAAGTAGAAAGGGGAATTAACAGCTATCCCATTCAAGGGTAACCAGCCGAATCATTAGGAACAGTTGTCGGACCCCGTCCCCCAAATCATCACCTCAGTTATCATTTATCATTTTGATGCTATTATATTGGCTAATTAAACTTAATTTGTAGCTGGATGGAACAATTATCGCTCCCACTGATCGCCAAACTTGGGGGAAGGGGCTGTTACAATGGATCCAATTCACAAAGCTGGTAGGGATTACCATACGGGGAAGTGGCATAATTGACGGAAGAGGGTCTGTCTGGTGGCAAGATTCCTCTTTTGATGAGCCTATAGATGACGAGATGAAACTCATTATCCCTATAAACAATACCAGAGCTGAAACAAAACCGACGCCGGTAATCAACACTATCTATCTTCTTCTATATTATGCTTTTCTAGTTGTAAAGAGAAAGGGAAATGATGGTGGTTCTCTCCTAAAATATGTGGTAGATAGGAAGCAATCTAGATGGGAAGATGCCAAGCATCAAGCCAACTGTAAGGCTCACTCACTTGCAAACTTCCAAGACGATTTCATTATGGCCCCATTATCCTCATCCTGTATAATTATTTGATATGTTATGTTGCACAGGCACTTAGGTTTTATGGAAGCTTCAATGCCACGGTCACGGGCATAACAATCCAAAACAGCCCCCAATGCCACCTCAAGTTTGACAACTGCGTGGGAGTCCTGGTGCATGATTTCAGCGTCTCATCTCCTGGAGACAGTCTTAATACTGATGGAATTCACTTGCAAAACTCCAAAGACGTTCTGATCCACAGCTCCAGTCTCTCTTGTGGTAATTACGTCATTTGTTATTCAATATTATCTCTTAAATGGCTAAGGGTGTGCATGTCTTGACTCTTGAGATAAGCTCAATACTCGACATTCAATCACCATGGCCACTTAAAAGGAGCATTTGATGGATAAAACACTAAATATTGACATAAAAATATGATAGGGTCCTGATCACATGCAACCTTTATCAATAATTTATTGCAGCAATTTGCAAGCATTCACAGTTCGACACGTCACAATAATCAATCTTTATCCTATATAATGTTGAGCTACCAATCATATGTTGTGTGTCCATAATGCTTATTTTGTTTGCATCGTTCAGTTTTTATTGTTTGTATAGGCACATAAATTGTTTCTATCCTTCCTCCTTATGTCTGCTTAGTCTAACACAGGGGTCCACTGATCTTGATATTGAATTATGATATCAGATAGATCCTAAAGTTAACCAACACCTTTCCCTTTCTCTCTTCCTTTTCCTTTTCCTTTTCCTTTTCTCTTTGATGGAATAATCCCCCTTCCAAGGTGTGACCCCAACCACACTACTCTGCCACTTTTGGCACCTACAAGTTGTAAAAGTTAAGACCTTTGGGAATGATGATACTGAAGTATAAGTGCAATACTTCGATCAATTTCGGCATGTTGATTCTAAACTTAATGGAAGAAACGAAAAAAGAAAACAACAAAAACAATGTAGATTATGAGCATGGAACATGAGAAAATCTTTGCTAAAAGTTTGTTGTTGGACTAAACAGGTGACTGATGATATTTGGTCTGATCCTTTCAGGAGATGATTGTGTTTCCATTCAAACTGGATGCTCGAACGTGTACATACACAATGTGAACTGCGGGCCAGGACATGGAATCAGCATCGGCAGTCTAGGAAAGGACCATACAAAAGCCTGTGTTTCAAACATAACGGTCCGAGATGTCACAATGCATAACACGATGAATGGTGTTAGAATCAAAACATGGCAGGTACTTGTTTTTACGAAGAAAAACTCGTGCTTCATCTTGTGTGAGGATGGGCCCTTATCAGCATGTTTTGAATTCAAAAGCAAGTTCTTAGTAGGTACTAAAGTCAATCGCTGTTGTCCTCCTGATACTCATAGGCTGAAATTAAATACCTTCAAAAGGTTCATAATGAACGGCATTCTTTCATGGACAGTCACAAGGCCCTATGAGTCATCTACAATGTTAATTGCTAAGCAGTGAATATCCATAAGTATTGATAGAATGCGAAATGATATGTTTACAGGGAGGATCCGGGTCTGTTCAGAACGTTCTCTTCTCAAACATTCAAATGTCCGAAGTTCAATTACCAATTGTGATCGACCAGTTTTACTGCGACAAAGCCAAATGTACGAATCAGACTTCAGCGGTGGCTCTATCAGGAATCAATTACGAGAAAATCAGAGGGACTTACACAGTGAAGCCTGTACACTTCGCCTGTAGCGACAACCTGCCATGCACGGATGTAACGCTGACGACTATAGAGCTGAAGCCACTGCAAGAGCGCTATCACTTATACGATCCTTTCTGCTGGCAAACTTTTGGAGAACTGAGAACCCCAACTTCACCTCCAATTGGGTGCTTACAGATCGGGAAGCCATCTAGCAACAGAGTTCAATATGAAGAAAATGATTCCTGCTAAGCTTAAGAGTATAATAGTAGATGATTTCTGAGTGGTCGGCATTTGTGACCCCTTTTTTTTATACTTTTAGATGGCTAGATTAAAGCATATATTGTGGGAAGATCTATCAGTTGATACCAATCTTTCCTTATAGGGTTAAATATATGTGAATCATATAGTATATAGGACTCGATGTTTAGCTCGAACTTTATAGTTGGTAACTAAAGAGCAGATTGTTTGATGATGCACATTTTCATTGCTTGGTGT

mRNA sequence

AATGAAAAAAATAAATGCCTGCCATTATCAAAATGGTATGTTGTCTAGCATTTGTCTGCACAGTCTCCTGAAGCTTTTACAGAGGCTCCACCTATTTAACCCCGCTTCACCTCCCACTTGCTTTGGCACTCTCAAAACCACTGACTCTCTCTCTGTTTCTCCTCTCATTCGCCACTGAAAATGATGAGTGGGATGAGTTCCAAAAGCTTAACACTTGTAGTTTTTATTGCTTTTCTTGTTTGGTCTTCAAACTTTGAAGCTTGCATTGCAAGGAGAGGAAAACACTGGAGACAGAGCAGGGAGGCCTCTGCTTCTCTATCCAAGAAGAAGGGCAGTCATGGCGGTAGCAAGAGCCACCATTCCGGTGGTTTCCACTCCAAAGCTCCACCGCCACACAATGCCTCTCCTCCCTCTCCAATTCCTCCTAAGCCCAAAGAAGAGATCGTTCAAACTCCACCCAAGAAAGGCTACAATGGCGGCGATTCTGCCATATTCAACGTTCTAGATTTTGGTGCTAAAGGCAATGGAGAGACTGATGACACTAAGGCATTTGAAGACGCTTGGGCGGCCGCTTGTAAGGTGGAGGGATCGACGGTCATGGTTCCCGCAGAATATGTGTTCTACGTAGGACCCATTTCCTTCTCTGGCCCATATTGCCAACCGAACATTGTATTTCAGCTGGATGGAACAATTATCGCTCCCACTGATCGCCAAACTTGGGGGAAGGGGCTGTTACAATGGATCCAATTCACAAAGCTGGTAGGGATTACCATACGGGGAAGTGGCATAATTGACGGAAGAGGGTCTGTCTGGTGGCAAGATTCCTCTTTTGATGAGCCTATAGATGACGAGATGAAACTCATTATCCCTATAAACAATACCAGAGCTGAAACAAAACCGACGCCGATAGGAAGCAATCTAGATGGGAAGATGCCAAGCATCAAGCCAACTGCACTTAGGTTTTATGGAAGCTTCAATGCCACGGTCACGGGCATAACAATCCAAAACAGCCCCCAATGCCACCTCAAGTTTGACAACTGCGTGGGAGTCCTGGTGCATGATTTCAGCGTCTCATCTCCTGGAGACAGTCTTAATACTGATGGAATTCACTTGCAAAACTCCAAAGACGTTCTGATCCACAGCTCCAGTCTCTCTTGTGGAGATGATTGTGTTTCCATTCAAACTGGATGCTCGAACGTGTACATACACAATGTGAACTGCGGGCCAGGACATGGAATCAGCATCGGCAGTCTAGGAAAGGACCATACAAAAGCCTGTGTTTCAAACATAACGGTCCGAGATGTCACAATGCATAACACGATGAATGGTGTTAGAATCAAAACATGGCAGGGAGGATCCGGGTCTGTTCAGAACGTTCTCTTCTCAAACATTCAAATGTCCGAAGTTCAATTACCAATTGTGATCGACCAGTTTTACTGCGACAAAGCCAAATGTACGAATCAGACTTCAGCGGTGGCTCTATCAGGAATCAATTACGAGAAAATCAGAGGGACTTACACAGTGAAGCCTGTACACTTCGCCTGTAGCGACAACCTGCCATGCACGGATGTAACGCTGACGACTATAGAGCTGAAGCCACTGCAAGAGCGCTATCACTTATACGATCCTTTCTGCTGGCAAACTTTTGGAGAACTGAGAACCCCAACTTCACCTCCAATTGGGTGCTTACAGATCGGGAAGCCATCTAGCAACAGAGTTCAATATGAAGAAAATGATTCCTGCTAAGCTTAAGAGTATAATAGTAGATGATTTCTGAGTGGTCGGCATTTGTGACCCCTTTTTTTTATACTTTTAGATGGCTAGATTAAAGCATATATTGTGGGAAGATCTATCAGTTGATACCAATCTTTCCTTATAGGGTTAAATATATGTGAATCATATAGTATATAGGACTCGATGTTTAGCTCGAACTTTATAGTTGGTAACTAAAGAGCAGATTGTTTGATGATGCACATTTTCATTGCTTGGTGT

Coding sequence (CDS)

ATGATGAGTGGGATGAGTTCCAAAAGCTTAACACTTGTAGTTTTTATTGCTTTTCTTGTTTGGTCTTCAAACTTTGAAGCTTGCATTGCAAGGAGAGGAAAACACTGGAGACAGAGCAGGGAGGCCTCTGCTTCTCTATCCAAGAAGAAGGGCAGTCATGGCGGTAGCAAGAGCCACCATTCCGGTGGTTTCCACTCCAAAGCTCCACCGCCACACAATGCCTCTCCTCCCTCTCCAATTCCTCCTAAGCCCAAAGAAGAGATCGTTCAAACTCCACCCAAGAAAGGCTACAATGGCGGCGATTCTGCCATATTCAACGTTCTAGATTTTGGTGCTAAAGGCAATGGAGAGACTGATGACACTAAGGCATTTGAAGACGCTTGGGCGGCCGCTTGTAAGGTGGAGGGATCGACGGTCATGGTTCCCGCAGAATATGTGTTCTACGTAGGACCCATTTCCTTCTCTGGCCCATATTGCCAACCGAACATTGTATTTCAGCTGGATGGAACAATTATCGCTCCCACTGATCGCCAAACTTGGGGGAAGGGGCTGTTACAATGGATCCAATTCACAAAGCTGGTAGGGATTACCATACGGGGAAGTGGCATAATTGACGGAAGAGGGTCTGTCTGGTGGCAAGATTCCTCTTTTGATGAGCCTATAGATGACGAGATGAAACTCATTATCCCTATAAACAATACCAGAGCTGAAACAAAACCGACGCCGATAGGAAGCAATCTAGATGGGAAGATGCCAAGCATCAAGCCAACTGCACTTAGGTTTTATGGAAGCTTCAATGCCACGGTCACGGGCATAACAATCCAAAACAGCCCCCAATGCCACCTCAAGTTTGACAACTGCGTGGGAGTCCTGGTGCATGATTTCAGCGTCTCATCTCCTGGAGACAGTCTTAATACTGATGGAATTCACTTGCAAAACTCCAAAGACGTTCTGATCCACAGCTCCAGTCTCTCTTGTGGAGATGATTGTGTTTCCATTCAAACTGGATGCTCGAACGTGTACATACACAATGTGAACTGCGGGCCAGGACATGGAATCAGCATCGGCAGTCTAGGAAAGGACCATACAAAAGCCTGTGTTTCAAACATAACGGTCCGAGATGTCACAATGCATAACACGATGAATGGTGTTAGAATCAAAACATGGCAGGGAGGATCCGGGTCTGTTCAGAACGTTCTCTTCTCAAACATTCAAATGTCCGAAGTTCAATTACCAATTGTGATCGACCAGTTTTACTGCGACAAAGCCAAATGTACGAATCAGACTTCAGCGGTGGCTCTATCAGGAATCAATTACGAGAAAATCAGAGGGACTTACACAGTGAAGCCTGTACACTTCGCCTGTAGCGACAACCTGCCATGCACGGATGTAACGCTGACGACTATAGAGCTGAAGCCACTGCAAGAGCGCTATCACTTATACGATCCTTTCTGCTGGCAAACTTTTGGAGAACTGAGAACCCCAACTTCACCTCCAATTGGGTGCTTACAGATCGGGAAGCCATCTAGCAACAGAGTTCAATATGAAGAAAATGATTCCTGCTAA

Protein sequence

MMSGMSSKSLTLVVFIAFLVWSSNFEACIARRGKHWRQSREASASLSKKKGSHGGSKSHHSGGFHSKAPPPHNASPPSPIPPKPKEEIVQTPPKKGYNGGDSAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVGPISFSGPYCQPNIVFQLDGTIIAPTDRQTWGKGLLQWIQFTKLVGITIRGSGIIDGRGSVWWQDSSFDEPIDDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATVTGITIQNSPQCHLKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDDCVSIQTGCSNVYIHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKCTNQTSAVALSGINYEKIRGTYTVKPVHFACSDNLPCTDVTLTTIELKPLQERYHLYDPFCWQTFGELRTPTSPPIGCLQIGKPSSNRVQYEENDSC
Homology
BLAST of CmaCh05G000150 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.7e-107
Identity = 214/488 (43.85%), Postives = 283/488 (57.99%), Query Frame = 0

Query: 42  ASASLSKKKGSHGGSKSHHSGGFHSKAPPP----HNASPPSPIPPKPKEEIVQTPPKKGY 101
           A A     KG HG +        H   PPP      A+PP  +P  P      +P     
Sbjct: 25  ARARYHYHKGRHGVT--------HPLPPPPPPPLETANPPDQVPSDP----YPSPDPAPG 84

Query: 102 NGGDSAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVGPISFSGP 161
           +     +F+V  FGA G+G  DDT AF+DAW AAC VE   V+ P   VF +    FSGP
Sbjct: 85  DSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP 144

Query: 162 YCQPNIVFQLDGTIIAPTDRQTWGK--GLLQWIQFTKLVGITIRGSGIIDGRGSVWWQDS 221
            C+P +VFQLDG ++ P   + W +     QW+ F +L G T  G G ++G G  WW   
Sbjct: 145 -CKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWD-- 204

Query: 222 SFDEPIDDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATVTGITIQ 281
                        +P    R      P GS+  G  P   PT +RF+ S N  V G+ IQ
Sbjct: 205 -------------LPCKPHRG-----PDGSSSSG--PCASPTMIRFFMSNNIEVKGLRIQ 264

Query: 282 NSPQCHLKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDDCVSIQT 341
           NSPQ H+KFD C GVL+++  +SSP  S NTDGIHL N++ V I++S +S GDDC+SI T
Sbjct: 265 NSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGT 324

Query: 342 GCSNVYIHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTWQGGSGS 401
           GCS+V I  V CGP HGISIGSLG  +++ACVSNITVR+  + ++ NG+R+KTWQGG+GS
Sbjct: 325 GCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGS 384

Query: 402 VQNVLFSNIQMSEVQLPIVIDQFYCDKAKCTNQTSAVALSGINYEKIRGTYTVK--PVHF 461
           V N+LF NIQM  V   I++DQ+YC    C N+TSAV +  + Y  I+GTY V+  P+HF
Sbjct: 385 VSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHF 444

Query: 462 ACSDNLPCTDVTLTTIELKPLQERYHLYDPFCWQTFGELRTPTSPPIGCLQIGKPSSNRV 521
           ACSD + CT++T++ +EL P +E   + DPFCW  +G+  T T PPI CL  G P     
Sbjct: 445 ACSDTVACTNITMSEVELLP-EEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEA 475

BLAST of CmaCh05G000150 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 3.6e-78
Identity = 160/420 (38.10%), Postives = 227/420 (54.05%), Query Frame = 0

Query: 86  EEIVQTPPK--KGYNGGDSAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGST-VMVP 145
           E ++Q P +  +        + +V +FGAKGNG TDDTKAF DAW  AC  +  T ++VP
Sbjct: 32  ESLLQLPRRQSRSTRPRSERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVP 91

Query: 146 AEYVFYVGPISFSGPYCQPNIVFQLDGTIIAPTDRQTW-GKGLLQWIQFTKLVGITIRGS 205
             Y   + PI  SGP C+  +  Q+ GTIIAP D   W G    +W+ F  L  +T+ G 
Sbjct: 92  ENYTCLLRPIDLSGP-CKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGG 151

Query: 206 GIIDGRGSVWWQDSSFDEPIDDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRF 265
           G ++G G  WW+ S                          P             PTAL F
Sbjct: 152 GTVNGMGQEWWERSC-------------------KHNHSNPCRG---------APTALTF 211

Query: 266 YGSFNATVTGITIQNSPQCHLKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHS 325
           +   N  V  + + +S Q H+   +C  V +    V +P  S NTDGIH+  S+ ++I +
Sbjct: 212 HKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDN 271

Query: 326 SSLSCGDDCVSIQTGCSNVYIHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTM 385
           +++S GDDC+SI    + + I N+ CGPGHGISIGSLGK  +   V +ITV    + +T 
Sbjct: 272 TTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTA 331

Query: 386 NGVRIKTWQGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAK-CTNQTSAVALSGINYE 445
           NGVRIKTWQGGSG V  ++F NI+M+ V  PI+IDQ+YCD  K C NQTSA+++  I++ 
Sbjct: 332 NGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFV 391

Query: 446 KIRGTYTVK-PVHFACSDNLPCTDVTLTTIELKPLQERYHLYDPFCWQTFGELRTPTSPP 500
            +RGT   K  +  +CSD+ PC ++ L  I+L+P        + FCW+ +G       PP
Sbjct: 392 HVRGTSASKEAIKISCSDSSPCRNILLQDIDLEP-SNGDGFTESFCWEAYGSSSGQVYPP 421

BLAST of CmaCh05G000150 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 8.0e-78
Identity = 154/371 (41.51%), Postives = 223/371 (60.11%), Query Frame = 0

Query: 104 IFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVGPISFSGPYCQPNI 163
           IFNV  +GA G+G+ D T+AF  AW AACK   + ++VP    F V  + F+GP CQP+ 
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGP-CQPHF 117

Query: 164 VFQLDGTIIAPTDRQTWGKGLLQWIQFTKLVGITIRGSGIIDGRGSVWWQDSSFDEPIDD 223
            F++DG I A  +  +W    + W+QF KL G T+ G G+IDG+G  WW           
Sbjct: 118 TFKVDGIIAAYQNPASWKNNRI-WLQFAKLTGFTLMGKGVIDGQGKQWWA---------G 177

Query: 224 EMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATVTGITIQNSPQCHLK 283
           + K +    N R       I ++ D      +PTA++F  S    + G+ + NSP+ HL 
Sbjct: 178 QCKWV----NGR------EICNDRD------RPTAIKFDFSTGLIIQGLKLMNSPEFHLV 237

Query: 284 FDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDDCVSIQTGCSNVYIH 343
           F NC GV +   S+++P DS NTDGI +  SK+  +  +++  GDDCV+I TG SN+ I 
Sbjct: 238 FGNCEGVKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIE 297

Query: 344 NVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTWQGGSGSVQNVLFSN 403
           ++ CGPGHGISIGSLG+++++A VS + V      +T NG+RIKTWQGGSG   ++++ N
Sbjct: 298 DLICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYEN 357

Query: 404 IQMSEVQLPIVIDQFYCDKAK-CTNQTSAVALSGINYEKIRGT-YTVKPVHFACSDNLPC 463
           ++M   + PI+I+QFYC  A  C NQ SAV +  + Y+ IRGT  T   +   CSD++PC
Sbjct: 358 VEMINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPC 401

Query: 464 TDVTLTTIELK 473
            D+ L+ I LK
Sbjct: 418 KDIKLSDISLK 401

BLAST of CmaCh05G000150 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.6e-76
Identity = 150/373 (40.21%), Postives = 216/373 (57.91%), Query Frame = 0

Query: 102 SAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVGPISFSGPYCQP 161
           + +FNV  +GA G+G+ D T+AF   W AACK   + ++VPA   F+V  + F GP CQP
Sbjct: 56  ATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGP-CQP 115

Query: 162 NIVFQLDGTIIAPTDRQTWGKGLLQWIQFTKLVGITIRGSGIIDGRGSVWWQDSSFDEPI 221
           ++ F++DGTI+A  D   W K    W+QF +L    + G+G+IDG+G  WW         
Sbjct: 116 HLSFKVDGTIVAQPDPARW-KNSKIWLQFAQLTDFNLMGTGVIDGQGQQWWA-------- 175

Query: 222 DDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATVTGITIQNSPQCH 281
             + K++    N R                   +PTA++   S + TV  +T+ NSP+ H
Sbjct: 176 -GQCKVV----NGRTVCNDRN------------RPTAIKIDYSKSVTVKELTLMNSPEFH 235

Query: 282 LKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDDCVSIQTGCSNVY 341
           L F  C GV +    + +P DS NTDGI +  SK   I    +  GDDC++I TG SN+ 
Sbjct: 236 LVFGECEGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNIT 295

Query: 342 IHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTWQGGSGSVQNVLF 401
           I ++ CGPGHGISIGSLG+D+++A VS++ V      +T NG+RIKTWQGGSG    + +
Sbjct: 296 IKDLICGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITY 355

Query: 402 SNIQMSEVQLPIVIDQFYCDKAK-CTNQTSAVALSGINYEKIRGT-YTVKPVHFACSDNL 461
            N++M   + PI+I+QFYC  A  C NQ SAV + G+ Y+ I GT  T   +   CSD++
Sbjct: 356 ENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSV 401

Query: 462 PCTDVTLTTIELK 473
           PCT + L+ + LK
Sbjct: 416 PCTGIQLSNVSLK 401

BLAST of CmaCh05G000150 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.6e-76
Identity = 152/373 (40.75%), Postives = 213/373 (57.10%), Query Frame = 0

Query: 102 SAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVGPISFSGPYCQP 161
           S   NV DFGAKG+G  DDTKAFE AW AAC    S V++  +  + V PISFSGP C+ 
Sbjct: 87  SKTVNVDDFGAKGDGR-DDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGP-CKS 146

Query: 162 NIVFQLDGTIIAPTDRQTWGKGLLQWIQFTKLVGITIRGSGIIDGRGSVWWQDSSFDEPI 221
            +  Q+ GTI A  DR  + K    W+ F  +  + + G G I+G G +WWQ+S      
Sbjct: 147 GLTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSC----- 206

Query: 222 DDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATVTGITIQNSPQCH 281
             +    +P  +                      PTAL FY S +  V  + I+N+ Q H
Sbjct: 207 --KTNKALPCKDA---------------------PTALTFYKSKHVIVKNLKIENAQQIH 266

Query: 282 LKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDDCVSIQTGCSNVY 341
           + FDNCV V   +  V++P +S NTDGIH+  ++++ I S  +  GDDC+SI  G   V 
Sbjct: 267 VSFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVR 326

Query: 342 IHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTWQGGSGSVQNVLF 401
           ++++ CGPGHGISIGSLG  +++A VS++ V    +  T NGVRIKTWQGGSGS  N+ F
Sbjct: 327 VNDITCGPGHGISIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKF 386

Query: 402 SNIQMSEVQLPIVIDQFYCDKAK-CTNQTSAVALSGINYEKIRGTYTVK-PVHFACSDNL 461
            N++M  V+ PI+IDQ YCD+ K C  Q+SAV +  + Y+ I+GT      + F CS   
Sbjct: 387 QNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRF 429

Query: 462 PCTDVTLTTIELK 473
           PC  + L  ++L+
Sbjct: 447 PCQGIVLEDVDLE 429

BLAST of CmaCh05G000150 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 717.6 bits (1851), Expect = 7.2e-207
Identity = 352/539 (65.31%), Postives = 421/539 (78.11%), Query Frame = 0

Query: 1   MMSGMSSKSLTLVVFIAFLVWSSNFEACIARRGKHWRQSREASASLS-----KKKGSHGG 60
           M   +S +S+T+++ +A LVWS   E CIARRG+HWR S  +S+ LS     KK  SHG 
Sbjct: 3   MRRSLSLRSITMMILMAVLVWSVTLETCIARRGRHWRHSHRSSSDLSDSLSNKKPKSHGN 62

Query: 61  SKSH--HSGGFHSKA---PPPHNASPP------SP-IPPKPKEEIVQTPPKKGYNGGDSA 120
           S  H  H+   H K+   P P   +PP      SP +   PK +    PP+KG     S 
Sbjct: 63  SHHHSSHNNNHHHKSKPKPKPKLKTPPKAHDNNSPVVSGPPKVQPPPLPPQKG-----SQ 122

Query: 121 IFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVGPISFSGPYCQPNI 180
           +FNV+DFGAKG+G+ DDTKAFE AW AACK+E S ++VP EY + VGPISFSGPYCQ NI
Sbjct: 123 VFNVMDFGAKGDGKCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVGPISFSGPYCQANI 182

Query: 181 VFQLDGTIIAPTDRQTWGKGLLQWIQFTKLVGITIRGSGIIDGRGSVWW-QDSSFDEPID 240
           VFQLDGTIIAPTD +TWGKGL+ WI FTKL GI ++G G+IDGRGS WW QDS F   ID
Sbjct: 183 VFQLDGTIIAPTDSKTWGKGLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQQDSPF---ID 242

Query: 241 DEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATVTGITIQNSPQCHL 300
            + KLI+P+NN+  +  P PI S LD +MPSIKPTALRF GSF   VTGITIQNSPQCHL
Sbjct: 243 SDTKLIVPLNNSANQNPPMPIRSELDERMPSIKPTALRFSGSFGVEVTGITIQNSPQCHL 302

Query: 301 KFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDDCVSIQTGCSNVYI 360
           KFD+CVGV+VHD +VSSPGDS NTDGIHLQN+KDVLIHS++L+CGDDC+SIQTGCSNV++
Sbjct: 303 KFDDCVGVVVHDIAVSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCISIQTGCSNVFV 362

Query: 361 HNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTWQGGSGSVQNVLFS 420
           HNVNCGPGHGISIGSLGK+ TKACVSNITVRDV MHNTM GVRIKTWQGG GSV+ ++FS
Sbjct: 363 HNVNCGPGHGISIGSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQGGVGSVKGIIFS 422

Query: 421 NIQMSEVQLPIVIDQFYCDKAKCTNQTSAVALSGINYEKIRGTYTVKPVHFACSDNLPCT 480
           NIQ+++VQ+PI I+QFYCD +KC NQTSAVA+ G+ YE+I+GTYTVKPVHFACSDN PC 
Sbjct: 423 NIQLNQVQIPITINQFYCDHSKCKNQTSAVAVEGVTYERIKGTYTVKPVHFACSDNFPCV 482

Query: 481 DVTLTTIELKPLQERYHLYDPFCWQTFGELRTPTSPPIGCLQIGKPSSNRVQYEENDSC 522
           DV L++IELKP+QE+Y +YD +CWQTFGEL TPT PPI CL+IGKP  N+VQ  ++D C
Sbjct: 483 DVQLSSIELKPVQEKYRMYDAYCWQTFGELNTPTLPPIDCLKIGKPPRNKVQ-SDHDVC 532

BLAST of CmaCh05G000150 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 713.8 bits (1841), Expect = 1.0e-205
Identity = 349/538 (64.87%), Postives = 419/538 (77.88%), Query Frame = 0

Query: 5   MSSKSLTLVVFIAFLVWSSNFEACIARRGKHWRQSREASASL-----SKKKGSHGGSKSH 64
           ++ +S+T++  +  LVWS+  E CIARRG+HWR    +S+SL     SKK  SH    +H
Sbjct: 7   LTLRSITMMTLVTILVWSATLETCIARRGRHWRHHHRSSSSLSDSLSSKKPKSH--ENNH 66

Query: 65  HSGGFHSKAPPPHNASPP-----SPIPPKPKEEIVQTPPKKGYNG----------GDSAI 124
           H     SK  P     PP     SP+  +P +  VQ PP                 DS  
Sbjct: 67  HHMSHKSKPKPKMKTQPPKSNDGSPVVSQPPQ--VQQPPPPHVQPPPTPLPLQPVEDSQQ 126

Query: 125 FNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVGPISFSGPYCQPNIV 184
           FNVLDFGAKG+G +DDT+AFE AWA+ACKVE ST+++P +Y+F VGPISFSGPYCQ NIV
Sbjct: 127 FNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVGPISFSGPYCQANIV 186

Query: 185 FQLDGTIIAPTDRQTWGKGLLQWIQFTKLVGITIRGSGIIDGRGSVWW-QDSSFDEPIDD 244
           FQL+G I+APTD ++WG GL+ WI+FTKL GITI+G+G+IDGRG+VWW QD   D PIDD
Sbjct: 187 FQLEGMIVAPTDTESWGGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDD 246

Query: 245 EMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATVTGITIQNSPQCHLK 304
           + KLI+P+NN+  E  P PI S L+ +MPSIKPTALRFYGS + TVTGITIQNSPQCHLK
Sbjct: 247 DFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVTVTGITIQNSPQCHLK 306

Query: 305 FDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDDCVSIQTGCSNVYIH 364
           FDNCV VLVHD +VSSPGDS NTDGIHLQN++DV+IH+++L+CGDDC+SIQTGCSNVY+H
Sbjct: 307 FDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVH 366

Query: 365 NVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTWQGGSGSVQNVLFSN 424
           NVNCGPGHGISIGSLGKD TKACVSNITVRDV MHNTM GVRIKTWQGG GSV+ +LFSN
Sbjct: 367 NVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSN 426

Query: 425 IQMSEVQLPIVIDQFYCDKAKCTNQTSAVALSGINYEKIRGTYTVKPVHFACSDNLPCTD 484
           IQ++EVQLPIVIDQFYCD +KC N TSAV++ G+ YEKIRGTYTVKPVHFACSD+ PC D
Sbjct: 427 IQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVKPVHFACSDSFPCID 486

Query: 485 VTLTTIELKPLQERYHLYDPFCWQTFGELRTPTSPPIGCLQIGKPSSNRVQYEENDSC 522
           V L+ IELKP+Q +YH+YDPFCW+TFGEL + T PPI CLQIGKP+ N V + ++D C
Sbjct: 487 VQLSGIELKPVQLQYHMYDPFCWKTFGELNSATVPPIDCLQIGKPARNGV-HSDHDIC 539

BLAST of CmaCh05G000150 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 541.2 bits (1393), Expect = 9.2e-154
Identity = 261/423 (61.70%), Postives = 305/423 (72.10%), Query Frame = 0

Query: 99  GGDSAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVGPISFSGPY 158
           GG S   NVLD GAKG+G +DDTKAFEDAW  ACKV  ST++VP+   F VGP+SF G  
Sbjct: 45  GGSSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKE 104

Query: 159 CQPNIVFQLDGTIIAPTDRQTWGKGLLQWIQFTKLVGITIRGSGIIDGRGSVWWQDSSFD 218
           C+  IVFQL+G IIAPT    WG GLLQWI+F  L GITI+G GIIDGRGSVWW D    
Sbjct: 105 CKEKIVFQLEGKIIAPTSASAWGSGLLQWIEFKALQGITIKGKGIIDGRGSVWWNDM--- 164

Query: 219 EPIDDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATVTGITIQNSP 278
                                       +  KMP  KPTALRFYGS   TV+GITIQNSP
Sbjct: 165 ----------------------------MGTKMPRTKPTALRFYGSNGVTVSGITIQNSP 224

Query: 279 QCHLKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDDCVSIQTGCS 338
           Q HLKFDNC+ + V DF+ SSPGDS NTDGIHLQNS+D +I+ S+L+CGDDC+SIQTGCS
Sbjct: 225 QTHLKFDNCISIQVSDFTTSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCS 284

Query: 339 NVYIHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTWQGGSGSVQN 398
           N+ IH+V+CGPGHGISIG LGKD+TKACVSNITVRDVTMH T NGVRIK+WQGGSGSV+ 
Sbjct: 285 NINIHDVDCGPGHGISIGGLGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQ 344

Query: 399 VLFSNIQMSEVQLPIVIDQFYCDKAKCTNQTSAVALSGINYEKIRGTYTVKPVHFACSDN 458
           V+FSNIQ+S V  PI+IDQ+YCD   C N+TSAVA+S INY  I+GTYT +PV FACSD+
Sbjct: 345 VMFSNIQVSNVANPIIIDQYYCDGGGCHNETSAVAVSNINYINIKGTYTKEPVRFACSDS 404

Query: 459 LPCTDVTLTTIELKPLQERYHLYDPFCWQTFGELRTPTSPPIGCLQIGKPSSNRVQYEEN 518
           LPCT ++L+TIELKP   +    DPFCW+  GEL+T T PPI CL+  K S        N
Sbjct: 405 LPCTGISLSTIELKPATGKASSLDPFCWKAHGELKTKTLPPIQCLKTEK-SPEAASRSNN 435

Query: 519 DSC 522
           D+C
Sbjct: 465 DAC 435

BLAST of CmaCh05G000150 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 399.8 bits (1026), Expect = 3.3e-111
Identity = 205/442 (46.38%), Postives = 281/442 (63.57%), Query Frame = 0

Query: 91  TPPKKGYNGGDSAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVG 150
           +P    Y   +  +FN+  +GAKG+G +DD+KA   AW AACKV G  V +PA   F V 
Sbjct: 53  SPAPAPYPSTNEGVFNIFSYGAKGDGVSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVK 112

Query: 151 PISFSGPYCQPNIVFQLDGTIIAPTDRQTW-GKGLLQWIQFTKLVGITIRGSGIIDGRGS 210
            ++  GP C+   V Q++G ++AP    +W    L QW+ F  +  +TI+GSG ++GRG 
Sbjct: 113 AVTLQGP-CKEETVVQIEGILVAPEKIGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGY 172

Query: 211 VWWQDSSFDEPIDDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATV 270
            WW   ++                T+   K           +P +KPTALRFY S N TV
Sbjct: 173 NWWNLDTY---------------QTQTRNK----------YIPPMKPTALRFYSSNNVTV 232

Query: 271 TGITIQNSPQCHLKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDD 330
             I+I NSP CHLKFD+  GV V++ ++SSP +S NTDGIHLQN+++V I  S+++CGDD
Sbjct: 233 RDISIVNSPLCHLKFDDSDGVKVNNITISSPENSPNTDGIHLQNTRNVEIQHSNIACGDD 292

Query: 331 CVSIQTGCSNVYIHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTW 390
           CVSIQTG SNV+IH++NCGPGHGISIG LGKD + ACVS+I V D+++ NT+ GVRIKTW
Sbjct: 293 CVSIQTGSSNVHIHHINCGPGHGISIGGLGKDKSVACVSDIIVEDISIQNTLAGVRIKTW 352

Query: 391 QGGSGSVQNVLFSNIQMSEVQLPIVIDQFYCDKAKCTNQTSAVALSGINYEKIRGTYTVK 450
           QGG G V+N+ FSNIQ+ +V++PIVIDQ+YCDK+KC NQT AV++SG+ Y  I G++TV+
Sbjct: 353 QGGLGVVKNLTFSNIQVKDVKVPIVIDQYYCDKSKCKNQTRAVSISGVKYNNIVGSFTVQ 412

Query: 451 PVHFACSDNLPCTDVTLTTIELKPL-----QERYHLYDPFCWQTFGELRTPTSP-PIG-C 510
           PV  ACS+N+PC DV L  I L+P       + +      CW ++G+ + P  P  IG C
Sbjct: 413 PVRIACSNNVPCMDVDLMDIRLRPSGGIRGLQTHQQQQALCWNSYGKTQGPLVPSSIGYC 468

Query: 511 LQ---IGKPSSNRVQYEENDSC 522
           L+   IG   S +V    +  C
Sbjct: 473 LRKSNIGGYYSQKVSRSYDKIC 468

BLAST of CmaCh05G000150 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 1.2e-108
Identity = 214/488 (43.85%), Postives = 283/488 (57.99%), Query Frame = 0

Query: 42  ASASLSKKKGSHGGSKSHHSGGFHSKAPPP----HNASPPSPIPPKPKEEIVQTPPKKGY 101
           A A     KG HG +        H   PPP      A+PP  +P  P      +P     
Sbjct: 25  ARARYHYHKGRHGVT--------HPLPPPPPPPLETANPPDQVPSDP----YPSPDPAPG 84

Query: 102 NGGDSAIFNVLDFGAKGNGETDDTKAFEDAWAAACKVEGSTVMVPAEYVFYVGPISFSGP 161
           +     +F+V  FGA G+G  DDT AF+DAW AAC VE   V+ P   VF +    FSGP
Sbjct: 85  DSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP 144

Query: 162 YCQPNIVFQLDGTIIAPTDRQTWGK--GLLQWIQFTKLVGITIRGSGIIDGRGSVWWQDS 221
            C+P +VFQLDG ++ P   + W +     QW+ F +L G T  G G ++G G  WW   
Sbjct: 145 -CKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWD-- 204

Query: 222 SFDEPIDDEMKLIIPINNTRAETKPTPIGSNLDGKMPSIKPTALRFYGSFNATVTGITIQ 281
                        +P    R      P GS+  G  P   PT +RF+ S N  V G+ IQ
Sbjct: 205 -------------LPCKPHRG-----PDGSSSSG--PCASPTMIRFFMSNNIEVKGLRIQ 264

Query: 282 NSPQCHLKFDNCVGVLVHDFSVSSPGDSLNTDGIHLQNSKDVLIHSSSLSCGDDCVSIQT 341
           NSPQ H+KFD C GVL+++  +SSP  S NTDGIHL N++ V I++S +S GDDC+SI T
Sbjct: 265 NSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGT 324

Query: 342 GCSNVYIHNVNCGPGHGISIGSLGKDHTKACVSNITVRDVTMHNTMNGVRIKTWQGGSGS 401
           GCS+V I  V CGP HGISIGSLG  +++ACVSNITVR+  + ++ NG+R+KTWQGG+GS
Sbjct: 325 GCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGS 384

Query: 402 VQNVLFSNIQMSEVQLPIVIDQFYCDKAKCTNQTSAVALSGINYEKIRGTYTVK--PVHF 461
           V N+LF NIQM  V   I++DQ+YC    C N+TSAV +  + Y  I+GTY V+  P+HF
Sbjct: 385 VSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHF 444

Query: 462 ACSDNLPCTDVTLTTIELKPLQERYHLYDPFCWQTFGELRTPTSPPIGCLQIGKPSSNRV 521
           ACSD + CT++T++ +EL P +E   + DPFCW  +G+  T T PPI CL  G P     
Sbjct: 445 ACSDTVACTNITMSEVELLP-EEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEA 475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z11.7e-10743.85Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ53.6e-7838.10Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432128.0e-7841.51Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E72.6e-7640.21Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P353362.6e-7640.75Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G10640.17.2e-20765.31Pectin lyase-like superfamily protein [more]
AT1G60590.11.0e-20564.87Pectin lyase-like superfamily protein [more]
AT5G14650.19.2e-15461.70Pectin lyase-like superfamily protein [more]
AT3G26610.13.3e-11146.38Pectin lyase-like superfamily protein [more]
AT1G48100.11.2e-10843.85Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 264..313
e-value: 490.0
score: 9.4
coord: 367..388
e-value: 0.54
score: 19.3
coord: 337..357
e-value: 4000.0
score: 2.9
coord: 396..417
e-value: 7900.0
score: 0.8
coord: 193..214
e-value: 1600.0
score: 5.7
coord: 314..335
e-value: 1700.0
score: 5.6
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 254..472
e-value: 8.9E-71
score: 238.6
coord: 137..216
e-value: 2.3E-8
score: 33.5
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 344..357
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 94..508
e-value: 3.9E-123
score: 413.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..87
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..98
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 7..510
NoneNo IPR availablePANTHERPTHR31375:SF192PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 7..510
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 102..510

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G000150.1CmaCh05G000150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0048015 phosphatidylinositol-mediated signaling
biological_process GO:0046854 phosphatidylinositol phosphate biosynthetic process
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0004650 polygalacturonase activity