CmaCh05G000080 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G000080
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial
LocationCma_Chr05: 29946 .. 33684 (-)
RNA-Seq ExpressionCmaCh05G000080
SyntenyCmaCh05G000080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAAATAGAGAAGAAAATATTTTCTGTCGTCTTTTTTTCCGTTTTGGCTTACCTTCAACAGTTGTACGTTTACCCGCACTAATTTGGGGATTGGTTATTTGGAAAAAGGGTAAATGTAATTAATTGTAAGTGATTTAAATTAATTTTGTGGAAGAAATGGAAGCGAACTCAAATTGTGAAGAGAAGAGATGAGGTGGTCCTGTGTCCATATCTTTGTTGTGTTGCGAGGGGGATCTGCCATCAGACAGCTCACACACACTTTGCCCGTTGTGACTCTTTTTAAAACTTGTCTTCTCCTTTTTCGTTTTCTTTTTCTTTTTCATTTTTTTTATAGCTAATCATTTATTATCTGTACTGTTGACTCACAGAATTCCACCACCGCCAACACTTGCTTACCATTAATAATAAGTCACTATTTTTTTTTTTCTTTTACCTAAAACTATGTGAAAGTGATGCAAAAGGGAATTTTGGAGTCTCAATTTTCATTTTTGTGATAAAAATTGGGAATACTTTGTTAAGTGAGTGATAAGATTTAGAGATGTGTTTTGGGGATGGTGAGGATAAAGAAGACAAGGATGGCGTTGGCTTCTATTTTGCGCTTGGGAATCTAAACATGTAGGCATCTTGTCACATGCGCCCACCCAACTATTTGTTCTTGCTCCTCTTTTCATTTGTAACATATTTACCAACTATTATTTATTTTTCTTTCCGTGTGCTACTTGTTGAAATCTCCTCAACATTTGAGTGGACGGCTTATAATAAAAAGGAGTTGGACTGCGACATATATACATATTTGTAATTATGGAATTTTGTTTATAGCGGCCTGTCCATTTACAAATTAAATTTAGACATGATTATAATTTCATTTTTGAAGATGGACCCAACTATTATATCTTTCCCTCAAACTTTCTTTTGAAGTTACAGATTGAAACAAAAAAAAAAAAAAAATTAAGAGCCCAAAATATTTTTGAAATGAATTACTAAGGATTAAAAGTAATTTTGAAATTGCCATCTCTATCTCTCTCTCTCACGGGATTTCATTAACTACCAGAAATTGTCTTCGCAAAATCTCAAATTGGCATAGCTCAGTGCACCTTTAGTTCCCCAAAAAGAGTGCAGATCTAAATTGAATAGCCTAATTTCGAATTAGTCAACAATTTTGAGGGCAAGCAGCCGCAGGCCCAGTTTGGAACAGCCCAAGGAAACTCCATAGGCTTAGTTTTCGTCCCAGAGGTCTATCAGATCGCTCCTCTTCCAGTTCTTACTTCCAAGTTCTGGCTTCACACAGACAGCCAAACAACTATGGCGGCTTGCTCTACCTGGAAAACCCTACGAATTTCCTCCTCTTCCGCAAGAACCCTCCTTCAACGTTCCTCCTCCTCCCCCTCTGCGTCTCATCTTGTGTCCAAACCTACTATGTCTGCGCCACTTCCTTCTGCAAAGCCGTCCGCATCTTCCCGCTTCTCTGTTCCGAAGCTTACCAACTTCAGGTATGTTTTTGGTTGTTTTGCTTTACTTATGGTATTCGCTCCTTTTCGATTTGGCGTTATGTTGGAAATATATAATTCAGTAATGTAATGCCGTTTTCTTCGGCTCCAAGATTTGGTATTTCTCAATTTCAATGGCCACAACCTTTTCCATTTCCTTTTTATAATAATAATAATAAAAAAAAAATCTAGTTTCTTGGATTTAATCCATGTTATCAGTTTCTTTCCCTAAAGAGGTGATCGTTTCTCTTTTCTAAAATCTAAGTAATTGCCCCTGTTCTTTAAATACATTTACTGGGTTAGCTCATCTATTTTTACCCATCCACGATCGAATTTTTGCGATTCTAGGCATTTCTATACTTTCTGAAGATAATCATCATGAAGACTAGTTTTTATGCTAAATGCTGCTGCTCCTTTAGGCTTCCAGTGGAGTTGTCAAGCGTGCAGTCTCTAATGCCATTGCACAGTGCTACTGCTTCTGCCTTGTTTACTTCCCTGCTTTCTTTGCATAACAACAGTTGGGGTTGTCTATCTGAAGGTAACCTTTCTGCCTTACTTCCTTGCGTCAATGTTAAATTACGCTATTAGAACATGCGATAAATTTATAGAATTGCCTTGCAATTGTGTTGACCTTTAATTTCTGAGTTTCTATATTAAATTACGCTATTAGAAGACTAATTGTTTGGTTAGAGTTAATTTAAGAACTTAGATTGTTTTTGCTAATTTTCCGTGAGTTTGAATTTTTGTTTGTTAACGAGGTTCAATACATATACTGGAGATTTGACATGCTAATTAGATGCCTGTGTAAGAATTTTTAATTCAATGTCTTCTGCCTCAGTACATCATTTTAAGTTATCACGAATTCCAGAAAACATACTAATCACAAACATTGATTTAACTCCATGAGTTGTTAAAGTTTCAAGTTTCTACTGATTCCTTAAAATATACTCATGATAAATAGTGATTTTATAACCCCATGAGTTGGCCAGTTTTCTACAATATCTAGATTGTATGGTTTTACATGGAGTATTTTCTTTCAAAGTGATAATGAACACAACCATTCATATAGAAAAAATTTATGGTAAACTTTGCTTTACAACAAAAAGCATCCCGTGAGGTAGGGAGAATTTGAGAGGATTAGGTTAATTCTTTCTACCATCCCTTTTGTGTATTATTACTCCCTATAAAGAGGAGTTCCCCTCATGTATTTCACATCTAGATCATAATAATGCTTCTTGTTGATTGATTCTTGGAGATTTCTCCTCTAGTCACTCTTTGGTTACATCACCCTGATTTCAGTTAATTCTAGAAATAATATGAAAAGAATTAGATAAATACATGATACAAATGGCTCTTTTGCAAGTATATGATGCTGAGTTTTAATATAATGATTAATTCAGGAATTGCCTTTCTTCAGAATTGGGGTTTTAAGCCTACTTGAAGACTGTTAATACTGATGTGAATCTTTATCTAGAAACCAAATTGTGGGAATAAAAACTTACGAAATTGTGAGAGTCAAATAATCTCTCTAAAAGGTTCCTAGATTTCTTGATTCTAAAATTCTTCAGTGATGTTCTTAAACTCCGATGGGGTTATGTCTCTCGCTGTTTAGTTTTCTGGTCCAAAATTAATTCATGGCCAGTAGATAAAAGCCATGAAATACCTACAAATATAAATAGAAATCCAAATTTATTTAATCGAAGTAGAAAGTAATCTGACCATAATACTCGATTGTGGTGGAACTTCACGTTTGAAGCACCTAGGTTCTAAGCTTTTTGGGCGAGGTTTTGGACGGTTTAGTGGCTAGGTGCTGAAATTATTCTTAGGATAATAACCAAATGCATAAGTGCACTCGAAATATTGAAAGAAAGAACACCCAACATTCAAGGATTACCCCACAGCGACATTTCCAAATAATTTCTTGAAAGATCTTTCAGCTTTTCATTCCTACTTTCATGCCTCTGCTTTCTCCATCCCCAAATAATGTTTTTTTACTTCTCAACATGTAATTTCTGGTGGGAAAAAACAATTTCATTTTTTTCCTTAAAAAATGAGTTATCTCTGAAAACATACTCGATGGGAAGCCAAAAATGTAAATTAATGAACAGTTATTAAACCTATTTGACTGAATATGTGTTTAAGAAATAGAAATGTTATGATTGTGTTCCTGTGGGTGTAAGTGGCTTCAAGTTCTACTCTTTATTTTTCTTCATATTTTGTGTCACACAGGATTTGCTACACCGCTATAA

mRNA sequence

ATGCAAAATAGAGAAGAAAATATTTTCTGTCGTCTTTTTTTCCGTTTTGGCTTACCTTCAACAGTTGTACGTTTACCCGCACTAATTTGGGGATTGGTTATTTGGAAAAAGGTCAACAATTTTGAGGGCAAGCAGCCGCAGGCCCAGTTTGGAACAGCCCAAGGAAACTCCATAGGCTTAGTTTTCGTCCCAGAGGTCTATCAGATCGCTCCTCTTCCAGTTCTTACTTCCAAGTTCTGGCTTCACACAGACAGCCAAACAACTATGGCGGCTTGCTCTACCTGGAAAACCCTACGAATTTCCTCCTCTTCCGCAAGAACCCTCCTTCAACGTTCCTCCTCCTCCCCCTCTGCGTCTCATCTTGTGTCCAAACCTACTATGTCTGCGCCACTTCCTTCTGCAAAGCCGTCCGCATCTTCCCGCTTCTCTGTTCCGAAGCTTACCAACTTCAGGCTTCCAGTGGAGTTGTCAAGCGTGCAGTCTCTAATGCCATTGCACAGTGCTACTGCTTCTGCCTTGTTTACTTCCCTGCTTTCTTTGCATAACAACAGTTGGGGTTGTCTATCTGAAGGATTTGCTACACCGCTATAA

Coding sequence (CDS)

ATGCAAAATAGAGAAGAAAATATTTTCTGTCGTCTTTTTTTCCGTTTTGGCTTACCTTCAACAGTTGTACGTTTACCCGCACTAATTTGGGGATTGGTTATTTGGAAAAAGGTCAACAATTTTGAGGGCAAGCAGCCGCAGGCCCAGTTTGGAACAGCCCAAGGAAACTCCATAGGCTTAGTTTTCGTCCCAGAGGTCTATCAGATCGCTCCTCTTCCAGTTCTTACTTCCAAGTTCTGGCTTCACACAGACAGCCAAACAACTATGGCGGCTTGCTCTACCTGGAAAACCCTACGAATTTCCTCCTCTTCCGCAAGAACCCTCCTTCAACGTTCCTCCTCCTCCCCCTCTGCGTCTCATCTTGTGTCCAAACCTACTATGTCTGCGCCACTTCCTTCTGCAAAGCCGTCCGCATCTTCCCGCTTCTCTGTTCCGAAGCTTACCAACTTCAGGCTTCCAGTGGAGTTGTCAAGCGTGCAGTCTCTAATGCCATTGCACAGTGCTACTGCTTCTGCCTTGTTTACTTCCCTGCTTTCTTTGCATAACAACAGTTGGGGTTGTCTATCTGAAGGATTTGCTACACCGCTATAA

Protein sequence

MQNREENIFCRLFFRFGLPSTVVRLPALIWGLVIWKKVNNFEGKQPQAQFGTAQGNSIGLVFVPEVYQIAPLPVLTSKFWLHTDSQTTMAACSTWKTLRISSSSARTLLQRSSSSPSASHLVSKPTMSAPLPSAKPSASSRFSVPKLTNFRLPVELSSVQSLMPLHSATASALFTSLLSLHNNSWGCLSEGFATPL
Homology
[Close]
BLAST of CmaCh05G000080 vs. TAIR 10
Match: AT1G70350.1 (unknown protein; Has 98 Blast hits to 98 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 98; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 76.6 bits (187), Expect = 2.4e-14
Identity = 55/108 (50.93%), Postives = 72/108 (66.67%), Query Frame = 0 

Query: 89  MAACSTWKTLRISSSSARTLLQRSSSSPSASHLVSKPTMSAPLPSAKPSASSRFSVPKLT 148
           MAA    + L+ SSSS + L  RSS++ SAS +     ++    S   S+S+R S+ +LT
Sbjct: 1   MAANGVRRALQASSSSGKILFGRSSAATSASKIGKSAGIAFGNGS---SSSTRPSLRRLT 60

Query: 149 NFRLPVELSSVQSLMPLHSATASALFTSLLSLHNNSWGCLSEGFATPL 197
             R+PVELS+  SL+PLHS TASAL TSLLSL N +W CLSEGFA+ L
Sbjct: 61  FSRVPVELSAGISLIPLHSVTASALLTSLLSLSNQNWSCLSEGFASTL 105

[Close]
BLAST of CmaCh05G000080 vs. TAIR 10
Match: AT1G55205.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13674.1); Has 137 Blast hits to 137 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 137; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 52.8 bits (125), Expect = 3.7e-07
Identity = 41/96 (42.71%), Postives = 58/96 (60.42%), Query Frame = 0 

Query: 102 SSSARTLLQRSSSSPSASHLVSKPTMSAPLPSAKP-SASSRFSVPKLTNFRLPVELSSVQ 161
           SS   +L Q+ S   +  H+ S P   +P+ S+ P SA+SR +     +FRLPVELS   
Sbjct: 11  SSKIHSLTQKLSK--TNVHISSLP---SPIKSSSPSSATSRIN----QSFRLPVELSCCL 70

Query: 162 SLMPLHSATASALFTSLLSLHNNSWGCLSEGFATPL 197
           S++PLHSA ASA   S LS+ + SWG + +G + PL
Sbjct: 71  SMLPLHSAIASARLISSLSVESKSWGLVPQGISMPL 97

[Close]
BLAST of CmaCh05G000080 vs. TAIR 10
Match: AT1G55205.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13674.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 48.1 bits (113), Expect = 9.2e-06
Identity = 39/91 (42.86%), Postives = 55/91 (60.44%), Query Frame = 0 

Query: 102 SSSARTLLQRSSSSPSASHLVSKPTMSAPLPSAKP-SASSRFSVPKLTNFRLPVELSSVQ 161
           SS   +L Q+ S   +  H+ S P   +P+ S+ P SA+SR +     +FRLPVELS   
Sbjct: 11  SSKIHSLTQKLSK--TNVHISSLP---SPIKSSSPSSATSRIN----QSFRLPVELSCCL 70

Query: 162 SLMPLHSATASALFTSLLSLHNNSWGCLSEG 192
           S++PLHSA ASA   S LS+ + SWG + +G
Sbjct: 71  SMLPLHSAIASARLISSLSVESKSWGLVPQG 92

[Close]
BLAST of CmaCh05G000080 vs. TAIR 10
Match: AT2G20585.3 (nuclear fusion defective 6 )

HSP 1 Score: 46.2 bits (108), Expect = 3.5e-05
Identity = 42/92 (45.65%), Postives = 59/92 (64.13%), Query Frame = 0 

Query: 103 SSARTLLQRSSSSPSASHLVSKPTMSAPLPSAKP--SASSRFSVPKLTNFRLPVELS-SV 162
           S AR++L+ +SS  +A+   S    ++   SA P   A++R S P L+  R PVELS  V
Sbjct: 8   SGARSMLRAASSRSAAA---STGRFASQAKSAPPLFRATARRS-PLLSPLRNPVELSFCV 67

Query: 163 QSLMPLHSATASALFTSLLSLHNNSWGCLSEG 192
           +SL+P HSATASAL TS LS+   ++G LS+G
Sbjct: 68  ESLLPYHSATASALMTSKLSISGQTYGWLSDG 95

[Close]
BLAST of CmaCh05G000080 vs. TAIR 10
Match: AT2G20585.1 (nuclear fusion defective 6 )

HSP 1 Score: 43.9 bits (102), Expect = 1.7e-04
Identity = 41/91 (45.05%), Postives = 58/91 (63.74%), Query Frame = 0 

Query: 103 SSARTLLQRSSSSPSASHLVSKPTMSAPLPSAKP--SASSRFSVPKLTNFRLPVELS-SV 162
           S AR++L+ +SS  +A+   S    ++   SA P   A++R S P L+  R PVELS  V
Sbjct: 8   SGARSMLRAASSRSAAA---STGRFASQAKSAPPLFRATARRS-PLLSPLRNPVELSFCV 67

Query: 163 QSLMPLHSATASALFTSLLSLHNNSWGCLSE 191
           +SL+P HSATASAL TS LS+   ++G LS+
Sbjct: 68  ESLLPYHSATASALMTSKLSISGQTYGWLSD 94

The following BLAST results are available for this feature:
BLAST of CmaCh05G000080 vs.
Match NameE-valueIdentityDescription
BLAST of CmaCh05G000080 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G70350.12.4e-1450.93unknown protein; Has 98 Blast hits to 98 proteins in 11 species: Archae - 0; Bac... [more]
AT1G55205.13.7e-0742.71unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G55205.29.2e-0642.86unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT2G20585.33.5e-0545.65nuclear fusion defective 6 [more]
AT2G20585.11.7e-0445.05nuclear fusion defective 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..141
NoneNo IPR availablePANTHERPTHR33156:SF2OS01G0738000 PROTEINcoord: 89..193
IPR043459NFD6/NOXY2-likePANTHERPTHR33156OS02G0230000 PROTEINcoord: 89..193

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G000080.1CmaCh05G000080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070475 rRNA base methylation
biological_process GO:0030488 tRNA methylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008173 RNA methyltransferase activity