CmaCh05G000050 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G000050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiondual-specificity RNA methyltransferase RlmN
LocationCma_Chr05: 22824 .. 26090 (-)
RNA-Seq ExpressionCmaCh05G000050
SyntenyCmaCh05G000050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTGAAGAGTCGAAGACCAACAACACTCGTTTATCCAGAGACTCGATCTAGTTAATTTAGCTGTTTCCGTACCTAAAAAAGGGGGAAAACCCCCCAAGGGCGCTGAAATACGGTGGAAACTGAAACTAGAGCGCGAAGAAGAAGATAGGTTGCGTTTGGATGAGACGCCTGTTCTTCCCCAAGCCTTCCATGGCCTCCTCCATCCTTGCCCCATCCCACTTTTATTCCCTTTCTATCCCTTCCGCACGTCTATGTCCTCCATTGCACCTAATTTCTTTCCTTTCTTCTGCTGGTCGCCGCTTTACGACTGCCTCTTCCGCATCACCTAAGCCGTTATTTGCTCTTGACGATTCTTCACCTGATTTCTGTCAGTTCTTGAGTATCTATGCCTGTTTTCTTACATTCTTTTATTTGTTTATTCGACTACTTATCCTTCGATTCTGTACATTTTGTTGCCATTTACATTCGGATGATACTTTTGCGGTCTCTTTTCTTATAGCTATGCCTGAAAAAAAGGAAATCCTGCACACTGATTCCAAGATGCTTCTAAAGGGTCTGTCTTACACTGAACTTGAGGCAAGTCCCTGCTAAACTCTCTCTCTTTCTCGTTTGCGCTTATCCGTCTCTCCTTCTTGCGCGCTTATTGAAGAAGACCCTTTTGCTCGGGTACGCGCATCCTAATTTTTGTTTTTACCCTAACATATACGCTGATAGAAATGGGTTAAAGCTCGAGGATACAGACCTGGTCAAGCTTTAATGCTGTGGAAACGTCTTTATGGGGATAACGTTTGGGCTCATTCCGGCGATGAATTAGAAGGTCTTCTTGCTTTCTCAATTTATTTCGAATACCAGCCTTTCTTTACATTTTAACGCTTTCATTGATAGCAGAATTTTTCCATCATCTGCTTTTCTCTTGTTGTAACTTGGAACTTATCTACTCAATCCAATTAATTTCTCCGTGCTGTCAAATCTTAGGTTTGAACAAAGATTTTAAGAAAATGTTGATTGAAAAAGCTGAATTCAGGGCGCTATCTTTGAGGGAAATTCTCCCTTCATCTGATGGAACGAGGAAGGTGTGTATAGTTACATTCTAGCAAGTGCTATATTATAATTATTCAATTTTCTTCTGGTTTCTTTGTGGGTTCTGGCTCCAGTGTACAAAATATCATTATACTTGGCTTTAGTTCTCTGCATTTGTGCTCCCAAGATTCGTCTGATTAATTTCAAAGCTCACAGGATATTACAGATTTTGTTCAATCTGGAAGATGGATTGATAATAGAAACTGTTGTTATACCTTGCGATAGAGGCAGGACTACTGTTTGCGTTTCAAGTCAAGTGGGCTGTGCTATGAACTGTCAATTCTGCTACACTGGCAGGCAAGCTTCAATTCTTCAATTTGATTAGATGCTATGCTTTCCGCATGATCTTAGTTGTAGTAAACATGAATTTTCGGATTGTTAGTTTTCAATTCATTTTTCAGGATGGGCCTGAAGAGACATCTGACTGCTGCTGAGATAGTAGAACAGGCAGTTTTTGCGAGGCGTTTGCTTACTTGTGAAGTTGGGTTAATTACTAATGTTGTGTTTATGGTATGTTTAAGATCTGAAACTATCTAACATACAAGATGGAAAACGAAATATATGGAAAAATTATCTTTTTTTGGAATTTTTCTAATTCTTTCAACGGGGCTGATCTACCCTATGTCCAGTTTGTGGTTTATTTACCAACCAAGATTTCGCTATGGGAGAAAATTAGTAGATTTTTAAATCCGAAAGGAGAGAATATGGTTGGTAGTAACACCTTATCAGAGGGGTGGTCCTAGTTGGTTTGGCACTCTTACAGGAATAATTCTACCTTATTTCAATCTGTGCCTGCAACGTTTTTTCATTTAATCATTGTAGACTATTCCAAGCCACACGTCCTTTATGCATTTAGCTGGAACAAAATTTGCAGGCATTTCTAGCATTCTTAGTTTTCTTTCCTGATAAGATTTTTATTGTTTCTTTGTTTTATGTCATATTGAAGATTTATGCTTGGTTTTTTGGGTCTTACTGAATACCTCTTCTTTTTCATTATTTTCATTATTAAAACCTGACATTTATAATGGTTGTCTCAGTGAGAACCATCATCTTTTTCCATGTTTTGATCAGAAAAACTGAAATTGAATGTTATTAGGGAATGGGAGAGCCACTTCACAACATTGACAATGTCATTAAAGCAGCAAATATAATGGTTGATGAACAAGGCCTTCATTTCAGTCCTCGCAAGGTCACTGTTTCAACCAGTGGACTTGTTCCCCAGCTCAAACGTTTCCTTCATGATTGTAACTGCGCTTTAGCCGTTAGTTTGAATGCAACTACTGATGAGGTACATGCTTAACGGTTGACATTACTCTAATGTGCGAAATAAAAAAAATATGAAGAATTCCCTTCTCTCTCACATGGCAAAATAAACGTTGCAGGTTAGAAATTGGATCATGCCAATTAACCGGAAGTATAAGTTAGGCTTGCTTCTTCAGACTTTACGTGAGGAACTTCGCTGCAAACACAATTACAAGGTTCTTTTTGAATATGTGATGCTTGCTGGGGTTAATGACAGGTTATTACAGCTTCTATGTTTTGGACTTATTCATTGTTTAAACCGGAGCTGTAGATGATGAGTTCATTTTATCAAGTTTATCTTTACAATCATTCTAATCTTTTGTTTTTTGTATCAATCGGTTGTATTGTAACATGCAGCATTGAAGATGCGAAGAGGATTGTTGATCTTGTCCAGGGTATTCCATGCAAGATTAACCTTATTTCATTTAATCCACATTGTGGATCTCAATTTAGACCTACCTGCAAGGAGAAGATGATCGAGTTTCGGAATGTTTTGGCTGCAGCCGGGTTGACTGTTCTCTTGCGACTAAGCAGAGGTGATGACCAGATGGCTGCCTGTGGTCAGTTAGGCAAACCTGGTACAATTCAAGCTCCTTTACTCCGTGTACCGGATCAATTCCAAATGGCAATGAAATTGGCTCCCTAGACCCTACTTTGTTCATTCGACAAGGTGTAGAAACATTCACCCCCAGATTGAGAACGTCATCCCTCATCTTATCCCAAATTTTGGCTCTCTTCAGTTTCGCTTTTCAACTTGTACCTTATTCTTGGGACAAATTTTGAATTTGGTATTTATAACCAGTGATATGGGCGTGGTAAGTTTGACACTAATTTGCAATGTAGTTGAACTTAACGACGATGTAGAAGTTGAAAACGTAGGTGC

mRNA sequence

TTCTTGAAGAGTCGAAGACCAACAACACTCGTTTATCCAGAGACTCGATCTAGTTAATTTAGCTGTTTCCGTACCTAAAAAAGGGGGAAAACCCCCCAAGGGCGCTGAAATACGGTGGAAACTGAAACTAGAGCGCGAAGAAGAAGATAGGTTGCGTTTGGATGAGACGCCTGTTCTTCCCCAAGCCTTCCATGGCCTCCTCCATCCTTGCCCCATCCCACTTTTATTCCCTTTCTATCCCTTCCGCACGTCTATGTCCTCCATTGCACCTAATTTCTTTCCTTTCTTCTGCTGGTCGCCGCTTTACGACTGCCTCTTCCGCATCACCTAAGCCGTTATTTGCTCTTGACGATTCTTCACCTGATTTCTCTATGCCTGAAAAAAAGGAAATCCTGCACACTGATTCCAAGATGCTTCTAAAGGGTCTGTCTTACACTGAACTTGAGAAATGGGTTAAAGCTCGAGGATACAGACCTGGTCAAGCTTTAATGCTGTGGAAACGTCTTTATGGGGATAACGTTTGGGCTCATTCCGGCGATGAATTAGAAGGTTTGAACAAAGATTTTAAGAAAATGTTGATTGAAAAAGCTGAATTCAGGGCGCTATCTTTGAGGGAAATTCTCCCTTCATCTGATGGAACGAGGAAGATTTTGTTCAATCTGGAAGATGGATTGATAATAGAAACTGTTGTTATACCTTGCGATAGAGGCAGGACTACTGTTTGCGTTTCAAGTCAAGTGGGCTGTGCTATGAACTGTCAATTCTGCTACACTGGCAGGATGGGCCTGAAGAGACATCTGACTGCTGCTGAGATAGTAGAACAGGCAGTTTTTGCGAGGCGTTTGCTTACTTGTGAAGTTGGGTTAATTACTAATGTTGTGTTTATGGGAATGGGAGAGCCACTTCACAACATTGACAATGTCATTAAAGCAGCAAATATAATGGTTGATGAACAAGGCCTTCATTTCAGTCCTCGCAAGGTCACTGTTTCAACCAGTGGACTTGTTCCCCAGCTCAAACGTTTCCTTCATGATTGTAACTGCGCTTTAGCCGTTAGTTTGAATGCAACTACTGATGAGGTTAGAAATTGGATCATGCCAATTAACCGGAAGTATAAGTTAGGCTTGCTTCTTCAGACTTTACGTGAGGAACTTCGCTGCAAACACAATTACAAGGTTCTTTTTGAATATGTGATGCTTGCTGGGGTTAATGACAGCATTGAAGATGCGAAGAGGATTGTTGATCTTGTCCAGGGTATTCCATGCAAGATTAACCTTATTTCATTTAATCCACATTGTGGATCTCAATTTAGACCTACCTGCAAGGAGAAGATGATCGAGTTTCGGAATGTTTTGGCTGCAGCCGGGTTGACTGTTCTCTTGCGACTAAGCAGAGGTGATGACCAGATGGCTGCCTGTGGTCAGTTAGGCAAACCTGGTACAATTCAAGCTCCTTTACTCCGTGTACCGGATCAATTCCAAATGGCAATGAAATTGGCTCCCTAGACCCTACTTTGTTCATTCGACAAGGTGTAGAAACATTCACCCCCAGATTGAGAACGTCATCCCTCATCTTATCCCAAATTTTGGCTCTCTTCAGTTTCGCTTTTCAACTTGTACCTTATTCTTGGGACAAATTTTGAATTTGGTATTTATAACCAGTGATATGGGCGTGGTAAGTTTGACACTAATTTGCAATGTAGTTGAACTTAACGACGATGTAGAAGTTGAAAACGTAGGTGC

Coding sequence (CDS)

ATGAGACGCCTGTTCTTCCCCAAGCCTTCCATGGCCTCCTCCATCCTTGCCCCATCCCACTTTTATTCCCTTTCTATCCCTTCCGCACGTCTATGTCCTCCATTGCACCTAATTTCTTTCCTTTCTTCTGCTGGTCGCCGCTTTACGACTGCCTCTTCCGCATCACCTAAGCCGTTATTTGCTCTTGACGATTCTTCACCTGATTTCTCTATGCCTGAAAAAAAGGAAATCCTGCACACTGATTCCAAGATGCTTCTAAAGGGTCTGTCTTACACTGAACTTGAGAAATGGGTTAAAGCTCGAGGATACAGACCTGGTCAAGCTTTAATGCTGTGGAAACGTCTTTATGGGGATAACGTTTGGGCTCATTCCGGCGATGAATTAGAAGGTTTGAACAAAGATTTTAAGAAAATGTTGATTGAAAAAGCTGAATTCAGGGCGCTATCTTTGAGGGAAATTCTCCCTTCATCTGATGGAACGAGGAAGATTTTGTTCAATCTGGAAGATGGATTGATAATAGAAACTGTTGTTATACCTTGCGATAGAGGCAGGACTACTGTTTGCGTTTCAAGTCAAGTGGGCTGTGCTATGAACTGTCAATTCTGCTACACTGGCAGGATGGGCCTGAAGAGACATCTGACTGCTGCTGAGATAGTAGAACAGGCAGTTTTTGCGAGGCGTTTGCTTACTTGTGAAGTTGGGTTAATTACTAATGTTGTGTTTATGGGAATGGGAGAGCCACTTCACAACATTGACAATGTCATTAAAGCAGCAAATATAATGGTTGATGAACAAGGCCTTCATTTCAGTCCTCGCAAGGTCACTGTTTCAACCAGTGGACTTGTTCCCCAGCTCAAACGTTTCCTTCATGATTGTAACTGCGCTTTAGCCGTTAGTTTGAATGCAACTACTGATGAGGTTAGAAATTGGATCATGCCAATTAACCGGAAGTATAAGTTAGGCTTGCTTCTTCAGACTTTACGTGAGGAACTTCGCTGCAAACACAATTACAAGGTTCTTTTTGAATATGTGATGCTTGCTGGGGTTAATGACAGCATTGAAGATGCGAAGAGGATTGTTGATCTTGTCCAGGGTATTCCATGCAAGATTAACCTTATTTCATTTAATCCACATTGTGGATCTCAATTTAGACCTACCTGCAAGGAGAAGATGATCGAGTTTCGGAATGTTTTGGCTGCAGCCGGGTTGACTGTTCTCTTGCGACTAAGCAGAGGTGATGACCAGATGGCTGCCTGTGGTCAGTTAGGCAAACCTGGTACAATTCAAGCTCCTTTACTCCGTGTACCGGATCAATTCCAAATGGCAATGAAATTGGCTCCCTAG

Protein sequence

MRRLFFPKPSMASSILAPSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPKPLFALDDSSPDFSMPEKKEILHTDSKMLLKGLSYTELEKWVKARGYRPGQALMLWKRLYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRKILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRMGLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGLHFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTCKEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLAP
Homology
BLAST of CmaCh05G000050 vs. ExPASy Swiss-Prot
Match: Q74E53 (Dual-specificity RNA methyltransferase RlmN OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=rlmN PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 7.6e-77
Identity = 163/342 (47.66%), Postives = 215/342 (62.87%), Query Frame = 0

Query: 81  DSKMLLKGLSYTELEKWVKARGYRPGQALMLWKRLYGDNVWAHSGDELEGLNKDFKKMLI 140
           D+ + +KGLS  ELE+++  +G    +A  ++K LY     A S  E+  L K+ ++ L 
Sbjct: 2   DTMIDIKGLSIDELERFLLGKGKERYRARQIFKWLYQRG--ATSFAEMTDLAKELRRDLE 61

Query: 141 EKAEFRALSLREILPSSDGTRKILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQ 200
           E A    LS   +  S DGTRK LF L+DG  +E+V+IP +  R T+C+SSQVGCAM C+
Sbjct: 62  ETARISTLSPEALEISRDGTRKYLFRLDDGCSVESVLIP-EEDRNTLCISSQVGCAMACE 121

Query: 201 FCYTGRMGLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANI 260
           FC TG   L R+LT AEIV Q    +R +      + N+VFMGMGEPL N+DNVI+A  I
Sbjct: 122 FCLTGTFRLTRNLTTAEIVNQVCAVQRDVP-----VRNIVFMGMGEPLANLDNVIRALQI 181

Query: 261 MVDEQGLHFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKL 320
           M+ + GL FS R++TVST+GLVP+++R        LAVSLNATTDE+R+ IMPINRKY L
Sbjct: 182 MLHDDGLQFSTRRITVSTAGLVPEMERLGRSVTVNLAVSLNATTDELRDRIMPINRKYPL 241

Query: 321 GLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCG 380
            +LL   R         K+  EYV+L GVND+++DAKR+V L+  IP KINLI FN H G
Sbjct: 242 AVLLDACR-RFPLPGRRKITIEYVLLGGVNDTLDDAKRLVRLLSDIPSKINLIPFNEHEG 301

Query: 381 SQFRPTCKEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQL 423
             FR   ++ +  F   L     TV+ R SRG D  AACGQL
Sbjct: 302 CSFRSPSQDAIDRFHRYLLDKHFTVITRSSRGADISAACGQL 334

BLAST of CmaCh05G000050 vs. ExPASy Swiss-Prot
Match: A1AL40 (Dual-specificity RNA methyltransferase RlmN OS=Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1) OX=338966 GN=rlmN PE=3 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 3.2e-75
Identity = 166/348 (47.70%), Postives = 214/348 (61.49%), Query Frame = 0

Query: 86  LKGLSYTELEKWVKARGYRPGQALMLWKRLYGDNVWAHSGDELEGLNKDFKKMLIEKAEF 145
           LK L+   LE++++ +G    +A  ++K +Y  +  A S  E+  ++KD +  L  KA  
Sbjct: 7   LKNLTLPALEQFLRGQGKERFRATQVFKWIYQHD--ARSFQEMTNISKDLRAELEAKAYI 66

Query: 146 RALSLREILPSSDGTRKILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTG 205
             L    +    DGTRK LF LEDG  +E+V+IP D GR T+C+SSQVGCAM C FC TG
Sbjct: 67  SNLEPEAVEVGGDGTRKYLFGLEDGNSVESVLIP-DEGRNTLCISSQVGCAMGCAFCLTG 126

Query: 206 RMGLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQ 265
              L R+LT AEIV Q +  RR +      I N+V MGMGEPLHN+DNVI A +IM+D  
Sbjct: 127 TFRLTRNLTTAEIVNQIMAVRRDVE-----IRNIVMMGMGEPLHNLDNVIPAIHIMIDGN 186

Query: 266 GLHFSPRKVTVSTSGLVPQLKRFLHDC-NCALAVSLNATTDEVRNWIMPINRKYKLGLLL 325
           GL  S R+VTVST GL P+++R   +  N  LAVSLNATTDE+R+ IMPINR+Y L  LL
Sbjct: 187 GLQLSNRRVTVSTCGLAPEMERLGRELPNVNLAVSLNATTDELRDRIMPINRRYPLKELL 246

Query: 326 QTLREELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFR 385
              R E       KV FEYVML G+ND++EDAKR++ L   IP K+NLI FN   G +FR
Sbjct: 247 SACR-EFPLPGRRKVTFEYVMLGGLNDTLEDAKRLLRLTSDIPNKVNLIPFNEFQGCEFR 306

Query: 386 PTCKEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQL-GKPGTIQAP 432
              +  +  F   L    +TV+ R SRG D  AACGQL GK    + P
Sbjct: 307 SPTRAAIDAFHKYLIDRHVTVITRDSRGSDISAACGQLKGKLDAARQP 345

BLAST of CmaCh05G000050 vs. ExPASy Swiss-Prot
Match: Q39S71 (Dual-specificity RNA methyltransferase RlmN OS=Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15) OX=269799 GN=rlmN PE=3 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 7.1e-75
Identity = 162/342 (47.37%), Postives = 214/342 (62.57%), Query Frame = 0

Query: 81  DSKMLLKGLSYTELEKWVKARGYRPGQALMLWKRLYGDNVWAHSGDELEGLNKDFKKMLI 140
           + K+ +K L+  +L  ++  +G    +A  ++K LY  +  A S  E+  L KD ++ L 
Sbjct: 3   NGKIDIKNLTLDDLIAFLAGKGKERYRARQIFKWLYQKD--ARSFAEMTDLAKDLRRDLE 62

Query: 141 EKAEFRALSLREILPSSDGTRKILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQ 200
           E A    L    +  S DGTRK LF LEDG  +E+V+IP +  RTT+C+SSQVGCAM C+
Sbjct: 63  ETAVISDLEPEAMEVSRDGTRKYLFRLEDGNTVESVLIP-EEDRTTLCISSQVGCAMACE 122

Query: 201 FCYTGRMGLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANI 260
           FC TG   L R+LTA EIV Q    RR +      + N+VFMGMGEPL N+DNV+KA  I
Sbjct: 123 FCLTGTFRLTRNLTAGEIVNQICAVRRDVP-----VRNIVFMGMGEPLANLDNVVKALKI 182

Query: 261 MVDEQGLHFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKL 320
           ++ + GL FS R+VTVSTSGLVP+++R   +    LAVSLNATTDEVR+ IMP+NR+Y L
Sbjct: 183 ILHDDGLQFSTRRVTVSTSGLVPEMERLGREVTVNLAVSLNATTDEVRDRIMPVNRRYPL 242

Query: 321 GLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCG 380
            LLL   R         K+  EYVM+ G+NDS+EDAKR+V L+  I  KINLI FN H G
Sbjct: 243 RLLLDACR-SYPLPGRRKITIEYVMIKGLNDSLEDAKRLVKLLSDISSKINLIPFNEHDG 302

Query: 381 SQFRPTCKEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQL 423
             F+   +  +  F + L +   TV+ R SRG D  AACGQL
Sbjct: 303 CSFKSPDQGAIDVFHSYLLSKHFTVITRSSRGSDISAACGQL 335

BLAST of CmaCh05G000050 vs. ExPASy Swiss-Prot
Match: Q3A2Z4 (Dual-specificity RNA methyltransferase RlmN OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=rlmN PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.7e-74
Identity = 157/356 (44.10%), Postives = 216/356 (60.67%), Query Frame = 0

Query: 81  DSKMLLKGLSYTELEKWVKARG---YRPGQAL-MLWKRLYGDNVWAHSGDELEGLNKDFK 140
           D+++ LK  +  EL +++   G   +R GQ +  ++ RL  D       D +  L+K  +
Sbjct: 8   DTRIDLKNFTLEELTEFLAGMGKERFRAGQVMRWMYHRLVDD------FDAMSDLSKVLR 67

Query: 141 KMLIEKAEFRALSLREILPSSDGTRKILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCA 200
             L ++A    L+      S DGTRK LF LEDG  IE+V IP D  R T+C+S+QVGCA
Sbjct: 68  AELHQRARISRLTPEATEDSRDGTRKYLFRLEDGETIESVRIPMDDNRATLCISTQVGCA 127

Query: 201 MNCQFCYTGRMGLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIK 260
           M C FC+TG  GL R+LT  EIV Q   A        G + N+V MGMGEPLHN+DNV+K
Sbjct: 128 MGCVFCHTGSFGLVRNLTPGEIVNQVCAA-----LADGPVNNIVLMGMGEPLHNLDNVVK 187

Query: 261 AANIMVDEQGLHFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINR 320
           A  I+   QGL +SPRKVT+ST+GLVPQ++         LAVSLNATTDEVRN +MP+N+
Sbjct: 188 ALQILYMPQGLDYSPRKVTLSTAGLVPQMQELGKRVRVNLAVSLNATTDEVRNRLMPVNQ 247

Query: 321 KYKLGLLLQTLRE-ELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISF 380
           +Y L  L+   R+  L  K   ++ FEY+++  VNDS +DA+R+V L+ GI  K+N+I F
Sbjct: 248 RYPLQQLMAACRQYPLHAKK--RITFEYILIRDVNDSDQDARRLVKLLHGIKAKVNIIPF 307

Query: 381 NPHCGSQFRPTCKEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAP 432
           N H  S+FR   +E++  F+  L   G+  + R S+G D  AACGQL    T+  P
Sbjct: 308 NEHSASEFRAPTEERISRFQGYLLDHGMVAIRRASKGQDISAACGQLKGKLTVSPP 350

BLAST of CmaCh05G000050 vs. ExPASy Swiss-Prot
Match: A5GEC2 (Dual-specificity RNA methyltransferase RlmN OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=rlmN PE=3 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 5.1e-73
Identity = 155/340 (45.59%), Postives = 207/340 (60.88%), Query Frame = 0

Query: 83  KMLLKGLSYTELEKWVKARGYRPGQALMLWKRLYGDNVWAHSGDELEGLNKDFKKMLIEK 142
           K+ +K  +  ELE ++  +G    +A  ++K LY  +  A    ++  L+KDF++ L + 
Sbjct: 3   KVDIKNFTLQELEAYIAGQGKERFRAKQIFKWLYQQD--AREFADMTNLSKDFRQELEKT 62

Query: 143 AEFRALSLREILPSSDGTRKILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFC 202
           A    L    +  S+DGT+K LF L DG  +E+V+IP D  RTT+C+SSQVGCAM C+FC
Sbjct: 63  AWISNLDAEAVEASADGTKKYLFRLADGNAVESVLIP-DEDRTTLCISSQVGCAMGCEFC 122

Query: 203 YTGRMGLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMV 262
            TG   L R+LT AEIV Q    +R        + N+VFMGMGEPL N+ NV+ A  I+ 
Sbjct: 123 LTGTFKLTRNLTTAEIVNQVCAVKRQEP-----VRNIVFMGMGEPLANLKNVVGALKILT 182

Query: 263 DEQGLHFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGL 322
           D  G  FS RKVTVSTSGLVP++          LAVSLNATTDEVR+ IMPINR+Y L  
Sbjct: 183 DPDGFQFSTRKVTVSTSGLVPEMAELGASVTVNLAVSLNATTDEVRDRIMPINRRYPLKE 242

Query: 323 LLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQ 382
           LL   +        + +  EYVM+ GVNDS++DAKR+V L+  IP K+NLI FN H G  
Sbjct: 243 LLAACKAFPLPSRRW-ITIEYVMIRGVNDSLDDAKRLVRLISNIPSKVNLIPFNEHDGCT 302

Query: 383 FRPTCKEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQL 423
           F+   ++ + +F   L    +TV+ R SRG D  AACGQL
Sbjct: 303 FQAPTQDSIDKFHKFLLDKHVTVITRSSRGSDISAACGQL 333

BLAST of CmaCh05G000050 vs. TAIR 10
Match: AT1G60230.1 (Radical SAM superfamily protein )

HSP 1 Score: 589.3 bits (1518), Expect = 2.5e-168
Identity = 304/452 (67.26%), Postives = 360/452 (79.65%), Query Frame = 0

Query: 1   MRRLFFPKPSMASSILAPSHFYS-------LSIPSARLCPPL-HLISFLSSAGRRFTTAS 60
           MRRL     + +++++ PS   S       L++PS  L   L + I F  S    F+  S
Sbjct: 5   MRRLGIITLTNSAAVVTPSLLSSTAAFPGTLTLPSPLLSDRLTNSILFHESRSLSFSCFS 64

Query: 61  SASPKPLFALDDSSPDFSMPEKKEILHTDSKMLLKGLSYTELEKWVKARGYRPGQALMLW 120
           S+S      L  S  ++   +       + K++LKG++Y  L++WV++ G+RPGQALMLW
Sbjct: 65  SSSSSSAAYL-PSLEEYPSAKGSVKRDDNQKVVLKGMTYASLQEWVQSHGFRPGQALMLW 124

Query: 121 KRLYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRKILFNLEDGLI 180
           KRLY DN+WA++ DELEGLNKD K+M+ E AEF ALS ++I  +SDGTRKILF L+DGL+
Sbjct: 125 KRLYKDNIWANNVDELEGLNKDLKRMISEHAEFGALSFKDIRSASDGTRKILFTLDDGLV 184

Query: 181 IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRMGLKRHLTAAEIVEQAVFARRLLTCE 240
           IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRMGLKR+LT AEIVEQAV+ARRLL+ E
Sbjct: 185 IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRMGLKRNLTTAEIVEQAVYARRLLSHE 244

Query: 241 VGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGLHFSPRKVTVSTSGLVPQLKRFLHDC 300
           VG ITNVVFMGMGEP HNIDNVIKAANIMVDE GLHFSPRKVTVSTSGLVPQLKRFL + 
Sbjct: 245 VGSITNVVFMGMGEPFHNIDNVIKAANIMVDENGLHFSPRKVTVSTSGLVPQLKRFLRES 304

Query: 301 NCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDS 360
           NCALAVSLNATTDEVRNWIMPINRKYKL LLL+TLRE L  +H YKVLFEYVMLAGVNDS
Sbjct: 305 NCALAVSLNATTDEVRNWIMPINRKYKLSLLLETLREGLSSRHKYKVLFEYVMLAGVNDS 364

Query: 361 IEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTCKEKMIEFRNVLAAAGLTVLLRLSRG 420
           ++DA+R+V+LVQGIPCKINLI FNPH GSQF  T ++KMI+FRNVLA  G TVL+R SRG
Sbjct: 365 MDDARRLVELVQGIPCKINLIQFNPHSGSQFIQTEEDKMIKFRNVLAEGGCTVLMRFSRG 424

Query: 421 DDQMAACGQLGKPGTIQAPLLRVPDQFQMAMK 445
           +DQMAACGQLG  G +QAP++RVP+QF+ A+K
Sbjct: 425 NDQMAACGQLGMIGAVQAPVMRVPEQFRTALK 455

BLAST of CmaCh05G000050 vs. TAIR 10
Match: AT3G19630.1 (Radical SAM superfamily protein )

HSP 1 Score: 196.1 bits (497), Expect = 6.2e-50
Identity = 130/365 (35.62%), Postives = 192/365 (52.60%), Query Frame = 0

Query: 92  TELEKWVKARGYRPGQALMLWKRLY--GDNVWAHSGDELEGLNKDFKKMLIEKAEFRALS 151
           +E++   ++ G  P  A+ +WK +    D VW    DE+  L      +L  K +    S
Sbjct: 10  SEIKSEFESAGINPKFAIQIWKYVIQNPDCVW----DEIPSLPSAAYSLLHSKFKTLTSS 69

Query: 152 LREILPSSDG-TRKILFNLEDGLIIETVVIPCDRG-------------RTTVCVSSQVGC 211
           L  +  SSDG T K+L  L++G  +E VV+  D               R+T+C+SSQVGC
Sbjct: 70  LHSLFHSSDGTTSKLLIKLQNGAFVEAVVMRYDTRLGMLGGKPRPGGIRSTLCISSQVGC 129

Query: 212 AMNCQFCYTGRMGLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVI 271
            M C FC TG MG K +LT+ EIVEQ V A R+       I N+VFMGMGEPL+N + V+
Sbjct: 130 KMGCTFCATGTMGFKSNLTSGEIVEQLVHASRIAD-----IRNIVFMGMGEPLNNYNAVV 189

Query: 272 KAANIMVDEQGLHFSPRKVTVSTSGLVPQLKRFLHDC-NCALAVSLNATTDEVRNWIMPI 331
           +A  +M++ Q    SP+++T+ST G+V  + +  +D    +LAVSL+A   E+R  IMP 
Sbjct: 190 EAVRVMLN-QPFQLSPKRITISTVGIVHAINKLHNDLPGVSLAVSLHAPVQEIRCQIMPA 249

Query: 332 NRKYKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLIS 391
            R + L  L+  L +  +     K+  EY+ML GVND  + A  + +L++     INLI 
Sbjct: 250 ARAFPLQKLMDAL-QTFQKNSQQKIFIEYIMLDGVNDQEQHAHLLGELLKTFQVVINLIP 309

Query: 392 FNP-HCGSQFRPTCKEKMIEFRNVLAAA-GLTVLLRLSRGDDQMAACGQL--------GK 430
           FNP    SQF  +  + +  F+ +L     +   +R   G D   ACGQL          
Sbjct: 310 FNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDISGACGQLVVNQPDIKKT 363

BLAST of CmaCh05G000050 vs. TAIR 10
Match: AT2G39670.1 (Radical SAM superfamily protein )

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-49
Identity = 137/407 (33.66%), Postives = 214/407 (52.58%), Query Frame = 0

Query: 25  SIPSARLCPPLHLISFLSSAGRRFTTASSASPKPLFALDDSSPDFSMPEKKEILHTDSKM 84
           S+P +R+    H ++   SA     T    SPKP+ A    +P           H DS +
Sbjct: 18  SVPISRMVVRRHSLATTFSAA---ATTVVPSPKPVSAKPARTP-----------HVDSHV 77

Query: 85  LLKGLSYTELEKWVKARGYRPGQALMLWKRLYGDNVWAHSGDELEGLNKDFKKMLIEKA- 144
           L+ G+S  EL++     G    +   L   +Y   V  +  ++   L   F+K L++   
Sbjct: 78  LI-GMSEPELQELAINLGQEGYRGKQLHHLIYKRKV--NKVEDFSNLPLTFRKGLVDGGF 137

Query: 145 EFRALSLREILPSSDGTRKILFNLEDGLIIETVVIPC---DRG--RTTVCVSSQVGCAMN 204
           +     + + + ++DGT K+L  LED L+IETV IP    ++G  R T CVSSQVGC + 
Sbjct: 138 KVGRSPIYQTVTATDGTIKLLLKLEDNLLIETVGIPVQDDEKGITRLTACVSSQVGCPLR 197

Query: 205 CQFCYTGRMGLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAA 264
           C FC TG+ G  R+L   EI+EQ +    +    V   TNVVFMGMGEP+ N+ +V+ A 
Sbjct: 198 CSFCATGKGGFSRNLQRHEIIEQVLAIEDVFKHRV---TNVVFMGMGEPMLNLKSVLDAH 257

Query: 265 NIMVDEQGLHFSPRKVTVSTSGLVPQLKRFL-HDCNCALAVSLNATTDEVRNWIMPINRK 324
             +   + +    R +T+ST G+   +K+   H     LAVSL+A    +R  I+P  + 
Sbjct: 258 RCL--NKDIEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKA 317

Query: 325 YKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQ--GIPCKINLISF 384
           Y L  +++  R+  + + N +V FEY +LAGVND +E A  + +L++  G    +NLI +
Sbjct: 318 YPLEAIMKDCRDYFQ-ETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPY 377

Query: 385 NPHCGSQFRPTCKEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQL 423
           NP  GS+++   K+ ++ F   L +  +T  +R +RG D  AACGQL
Sbjct: 378 NPIEGSEYQRPYKKAVLAFAAALESRKITASVRQTRGLDASAACGQL 401

BLAST of CmaCh05G000050 vs. TAIR 10
Match: AT2G39670.2 (Radical SAM superfamily protein )

HSP 1 Score: 192.6 bits (488), Expect = 6.9e-49
Identity = 139/414 (33.57%), Postives = 218/414 (52.66%), Query Frame = 0

Query: 25  SIPSARLCPPLHLISFLSSAGRRFTTASSASPKPLFALDDSSPDFSMPEKKEILHTDSKM 84
           S+P +R+    H ++   SA     T    SPKP+ A    +P           H DS +
Sbjct: 18  SVPISRMVVRRHSLATTFSAA---ATTVVPSPKPVSAKPARTP-----------HVDSHV 77

Query: 85  LLKGLSYTELEK------WVKARGYRPGQA-LMLWKRLYGDNVWAHSGDELEGLNKDFKK 144
           L+ G+S  EL++       +   GYR  Q   +++KR        +  ++   L   F+K
Sbjct: 78  LI-GMSEPELQELAINLVLIFQEGYRGKQLHHLIYKRK------VNKVEDFSNLPLTFRK 137

Query: 145 MLIEKA-EFRALSLREILPSSDGTRKILFNLEDGLIIETVVIPC---DRG--RTTVCVSS 204
            L++   +     + + + ++DGT K+L  LED L+IETV IP    ++G  R T CVSS
Sbjct: 138 GLVDGGFKVGRSPIYQTVTATDGTIKLLLKLEDNLLIETVGIPVQDDEKGITRLTACVSS 197

Query: 205 QVGCAMNCQFCYTGRMGLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNI 264
           QVGC + C FC TG+ G  R+L   EI+EQ +    +    V   TNVVFMGMGEP+ N+
Sbjct: 198 QVGCPLRCSFCATGKGGFSRNLQRHEIIEQVLAIEDVFKHRV---TNVVFMGMGEPMLNL 257

Query: 265 DNVIKAANIMVDEQGLHFSPRKVTVSTSGLVPQLKRFL-HDCNCALAVSLNATTDEVRNW 324
            +V+ A   +   + +    R +T+ST G+   +K+   H     LAVSL+A    +R  
Sbjct: 258 KSVLDAHRCL--NKDIEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREK 317

Query: 325 IMPINRKYKLGLLLQTLREELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQ--GIPC 384
           I+P  + Y L  +++  R+  + + N +V FEY +LAGVND +E A  + +L++  G   
Sbjct: 318 IVPSAKAYPLEAIMKDCRDYFQ-ETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTY 377

Query: 385 KINLISFNPHCGSQFRPTCKEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQL 423
            +NLI +NP  GS+++   K+ ++ F   L +  +T  +R +RG D  AACGQL
Sbjct: 378 HVNLIPYNPIEGSEYQRPYKKAVLAFAAALESRKITASVRQTRGLDASAACGQL 404

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q74E537.6e-7747.66Dual-specificity RNA methyltransferase RlmN OS=Geobacter sulfurreducens (strain ... [more]
A1AL403.2e-7547.70Dual-specificity RNA methyltransferase RlmN OS=Pelobacter propionicus (strain DS... [more]
Q39S717.1e-7547.37Dual-specificity RNA methyltransferase RlmN OS=Geobacter metallireducens (strain... [more]
Q3A2Z42.7e-7444.10Dual-specificity RNA methyltransferase RlmN OS=Pelobacter carbinolicus (strain D... [more]
A5GEC25.1e-7345.59Dual-specificity RNA methyltransferase RlmN OS=Geobacter uraniireducens (strain ... [more]
Match NameE-valueIdentityDescription
AT1G60230.12.5e-16867.26Radical SAM superfamily protein [more]
AT3G19630.16.2e-5035.62Radical SAM superfamily protein [more]
AT2G39670.11.4e-4933.66Radical SAM superfamily protein [more]
AT2G39670.26.9e-4933.57Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 149..428
e-value: 1.2E-108
score: 364.5
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 190..354
e-value: 1.3E-16
score: 61.5
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 181..414
score: 30.313931
NoneNo IPR availablePFAMPF13394Fer4_14coord: 194..294
e-value: 1.9E-5
score: 24.7
NoneNo IPR availableGENE3D1.10.150.530coord: 83..146
e-value: 2.9E-5
score: 25.6
NoneNo IPR availableSFLDSFLDG01062methyltransferase_(Class_A)coord: 81..424
e-value: 0.0
score: 383.2
NoneNo IPR availablePANTHERPTHR30544:SF5RADICAL SAM SUPERFAMILY PROTEINcoord: 69..430
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 194..362
e-value: 4.99175E-8
score: 51.1801
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 183..403
IPR027492Dual-specificity RNA methyltransferase RlmNTIGRFAMTIGR00048TIGR00048coord: 82..423
e-value: 1.0E-101
score: 338.4
IPR004383Ribosomal RNA large subunit methyltransferase RlmN/CfrSFLDSFLDF00275adenosine_C2_methyltransferascoord: 81..424
e-value: 0.0
score: 383.2
IPR040072Methyltransferase (Class A)PANTHERPTHR3054423S RRNA METHYLTRANSFERASEcoord: 69..430

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G000050.1CmaCh05G000050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070475 rRNA base methylation
biological_process GO:0030488 tRNA methylation
biological_process GO:0006364 rRNA processing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding