CmaCh04G030310 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G030310
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionQueuine tRNA-ribosyltransferase accessory subunit 2
LocationCma_Chr04: 19780914 .. 19786439 (-)
RNA-Seq ExpressionCmaCh04G030310
SyntenyCmaCh04G030310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGGGAGGCGACTAGGGTTGAAGTGGCGTCCATATTTAAGAGCCCTCCACTATTTCAAACAGATTTTAGCGCCACACATCTGTGTCTGTTTTGAGGCAGAATGAAATTCGCGGTGAAGGCGTGGAGCAACTGCAAGGCGCGCACCGGATGTCTACAGCTCGGAAACTGCCCTACCGCCATTGATACTCCTGCTTTACTTCTCTCTACTTGTAAAGGACTGCCTAATTTCATCTCTCCTGATCTTCTTCCGTCTCTCCCTTCCCCTGATTCTCACCTCCTCCAGTTCAGTCCTCTTCACTTGTAAGTATTCTACACTATTCCTACTCTTTCATCTCCATTCCGTCGTTCTCTTTTAATTCACTTTGTGTCGATGTTGCGATGAATCTGCTTATTCGCGTTCATTGTGTTTTTCAATTGGAGCGCCTCAGCCGGAGAGACTGCTCTACTTTGTTGCATTGATTTTCTTTTTATTTATTCGTTCTCTCACTTCGTAGGATTGTCTGTTTTGAGTACGTCTCGTGACCTGGAAACTATTCTACAGTCATGAGAATACGACTGGATAAAGCATGAGATCAGAAGTTCGTACTAGGTGATAATCGTATGATGCTTTTTGAAATGTTCTAGATTACTAAAACCAAGAGGAATGTGTCATGATGATTGCAGCTGATAGTACTATATTTAGGAAAGAATGCTGAACGCAGACTGTGTGTTCACGGAAATTGAAATGATTACTATGTCTATGTGGTACTTAAATAACCGAACAGTTGAGTTTGGGAAGCAACGACTACTTTACGTTGTCGGACTTATGCCTTAGGCTCTTCCGAAATAGTTTATCGGTGGTTTTTTCTGTAATCATTTGTATCCATGAAACAACTGTCAAGTAATTTATGGAATCCATTACTTTTATCTGTCTTTTGTCCTTGATTCAATTTCCATACTAGATCAATTTTTAACTTGTACATGACACGCTGTAGTGAAAATTTGGTTCTCTTCAGAATTATGAAGTGAGTTCTATTTTTGTACTGGTAAGTGGTACATTAATTGACTCTGTACGTTGTTTCTTGATGTTGTTGGCGGTGTTTGTTCAGACACTTTATACTCCTGTTACAGCTGTCTTGACTCTGGTTTTATTTTCATGATTCCATCTTTTATTAATTAATCGGCTTTCCTTCACGGTGTAATCAAGAAATAAGTCCGGCAGAAAAGAAATAAAATTTTCAATTATCACATGAAGATAGTCGTGTTTATTTGGTTTTTTGTTTCTTGTCAGCCACATTGACTTTTTAATCCTTTAATTCTTAGCTTGGAAGGTCCTTCATTGAAAACGATATCAAGTATTGGAGGGCTGCAGCATTTACTTGGGTTACATGAATATGTATTTGCAGCAGTAGCAAGGGATTCTGTTCAGTGCCTTCCAGAATGTAATGCTTCCAATAAATTTGGAGCATCTTTTGAGACTACTTGTGGTCGTCGTTTGGTAATGAGATTTCTGGTTTGGAAAATAAGTTTCTAAGTTTGTGGTTGTTGTTTTGTATCAACTTCTGCTTGCCCTTCCATTACTATTTTGTTTGAAAAAGAAGTTTATGAGTTTGCATTGTAATTGTAGATAAAACCTGTTGAGTATGTTGAATTGATTTCGTCCATGAGACCAGACATTTGGGCCTCCTTGGCTGATGAAGTGCCATCGTGGGTTTCAGATAAAAGGAATAAAACATCAGTGGATCGAACTGTGAGATGGCTCGATGAATGCATCAATTTGAACTCAGTAAGACTGCTAGAATGTCCCTTTTATGGTATTAGTTGAACTCTGCTAGATGTCTAATGTTGAGAAATGTAGGTCAAGTATGTTTGTGTGTTTTTAGACTTCAACTTGTGAATAGGCCCACGAAGAGAAAAGAAGCTGAGAAAAGCAGTAGACAAGAAATTTTACTGACGACTAAAAACTGCCTCCACGTTAAGTCTGTGGGGACCATCTGGTTGTGCAATTCTATATGGAAATTTATAGCACGCAGTAATTAGATAGAGGAAAATGAAGCAGCTTAGAATGTGGACCATAATTTCCAAAGCAAGCTGGCGAAAGGAGGGAATAAGAATTCTGGTCTCTTTTAAATAGATAAAGATGGCATAATTCTACAAATAAAATGAGGATATCATGAAAATATTTTAGGAATTCATCGAAAACCTGCTCATGGTTGGTGATTGTCGAATATTTTCCGAATCGGAATATTGGAGTGATTGATTGGTAAGATTTTATTCACATTTTCTAGGAGTAGATTCCCAACTGTAAAAGGGTTAGGGTGCATAAAGTGTCTTGCCTAGATAGTACGGGTGAAGCTTTGAGACAAGGAGCATCTTAAAAAAATTGACTGGAATGTGGTTTCCTAATAGTATTTCGAAAGAGGTTCTTAACAAATACACAAATGAAACCAACATTTTCCCTATTCCTCTGGCTTATAATTAGAGAAGCCTCCGTTCATTTTGTTGTAACTTTAGTAACTTTTAGATCCTCCTAGATGAGTGTTGTTATGTGTTTGTTTTGAGGAACAAGGATTCTACCTCCCATGCCTTTTGGTTACATTCTCCTTCTATGTTGGGAATGAAGTTTTCATTTCTAACTAAAAAAAAGAGTTTCTACCTCTTAGTTTTTGTGCCCAAAACAAGTTTCTTAAGTATTTTGTGCCAAATGGTTCCTCAGAGTTAAAATGTTCTGGATATTATGGCTGATGACTTTTCAGTGCTTCCAGAGTATCATTCTTATGGCTTTCAGAATTCCCTCAAAAACAAGAGCGTTGGGCCATATACATTCAGTAAAATAAGTAAAGGTTGATTCTTACAATTTCTAGAGTCATCTTACGTAATATGACTGTTTTGTTTAGGACTCATCGATGCAAAAGCAATATGAAAACCCTGTCATGATCAAGTGATAGTATTTAGTTTTTTTGTCTAGATTTGGTGCTTTTTACATTTTTCTTTGCCTAGTAATGTCATGAGTGATTTGGAACAATCAATTTATTATTATAATGAAGACAGGTGGGGCTATTTTTGGAGCTATTGTTGGAGGCAATGATATGGAAGAACGCCAAAGATGTGCTTTAGAGGTGGTTAAGCGTAATGTATCAGGTTCAATAACTTTTTCTTTGATATATAAAGGTGAATAGTGGCAATTAGCTCAAGATTCATAGCTTGAGAACTAATTTTGAGCAATATCAATTTTTAGCATCTCCCAAAATTTGTTTAGGCTATCAGAGCTCTGAACAGCCAGTTTGTATCATGTCTTTAAACACAACATGTTTTTAAATCATTACCATACAAATAAAATTTTGAACAGAATGCCAATATTTTCTTTCCTCTGATTCTCGTCATAACACCCCTGACCCTCTCTCGTATGTAAGGAATGATTATTTCCCTGGCTTCCCACATAAATGTTTCTCAACTCTAGTTTGTTCAGTTAAATTTATCATTTCTATCTTTGCTTCCAATTTCTTGTTCTAATTTCTCAGTTTCATCAAACAAATTTTGGTAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATAGATGAGCGTCCTGCCCTACTTGATGCTGTTACGGTAATAATTTGGCTTATAAAATTGTTAGGTTAGTCTTTTAGGCGTCCCCGTCCATTTCTTGCACCAACTAGGGTGTGGATGGTTTGCATGCGAACCCTACTAGTTAATGAGTTGAACTTTGAAGCTTAATAGGTAGCAAGTTGGATTCGTTTGTGTATACCTTTCTTTGTTAAAACAATTCTTTGGACCTTGCTCTTGTTTTTTGAAGTTTTTTTTTTTCTTTTATCATTACTTTCAACCCTTGATGAATTTTTTCCCCTAACAAAATAAAAAAGGTAACTGAGCTGTGATCGATTCCCTTTACTTCCATATCCCTTTTCTCTCTCTTTCTCCCCGTCATTTTTCCTGCACTTTCCATGTGTGTATGCCTGTATATATATGTGTGTATATATTATCTTTATCATCTGCGTTCCAAGTACCTCAATTCAATGCTCTGTTTCTGTGATGTCCATTTTCTATTCTACATTTTCTCCCATGAGTTGTGAAGCAAAAGCATCATAAACCATATCAGAATTTATCTTTTTTGAAAAAGAAGCGTGCTTCTGGTAATTTATTTCATTGTTTGAACAGGATGTCTTGCCAGAGGAATTGCCACGTATGATTTGTGGGCTCGGACTTCCAGGTATAGTTTTTCAATCTTCTAGGTTGAGTTGCTTGAAGTATACAATCCTGTTTCGACTTTCGATTGGATTTCTGTGTCCAGCCTATGGAAGCTGAAAATACATATTTTTAATATTTTTTGTAGCTTGTGACTAAGCATTTTGGTTCAATCATTTTTGTCACTCTTTATGATGACCACGTAGTGCATTTTGTCATTGTCGCTTGTCAATCAAATTAGTTTTATTAAATATCATTATTCTTATTATAGTCTATACACACACACATAACGTAACCTCAAAGTTCCAAATCTATGTACTTTGAGCTTCTGTAGTTTATAAATTAGTGAATTCTTCCCTTGAATCAATGAAATAGGCATCAAATGTGAGCTCAATATCATAACCATACTGTACGACTCAATTAGTTAGCTACCCCTGATAAAATGTTGGATGGCATCTGACCAATTAGAGGTTTAATCGTTCATCTTGGTTGCCCCATCCTATAATTAATCCACCAAGTTTTCTATTTTACATTGGGTTCTCGCTTCAAACAATAATTTGTTTGGATATATGTTTTCTTTGATGTTGCAGAGGAGATCTTGCAGGGTATTGCTGCTGGAGTCGATCTCTTTGACTCTGCGTATGCTATCCATTTTGAATATGGTTTATCTTTCTGTGATTGTCATTGCTGAATGAGCTCTATAAATTGTTTAATTACTGTGTTGCAGGTACATATATCACTTGACCCTTGGAGGCTTTGCTCTCACCTTCCCACTGAATGGAATTGCGGAGAAACGGCCTGACACGCACCTGAACGACGATGCAAGTGATCGGACAAAAATCAACTTAAGAGCAACACTTTATCGGTAAGTTTGACAATATTTCACATGCTTGGTATTGATTTGGCAATATTTAATTCCTTATTATCTAATCGTAGATCCTAATTTGCATTGTTATATTTGCTAAAAATGGGTTATTTGTTTTGTTCAGGAAAGATACATCACCAATTCTCGAGGGGTGTAACTGCTACGCTTGCCTGAATCATACCAAGGCTTATATAAATCACTTGCTAAATGTCCACGAAATGCTGGCTCAAATACTGCTGGAGATGTATGAGAATTTGACTACTTAGAAAATATTTAGTCGTCTGTCTCTATTTGCATTCTCACAGTAATGGTTCTCATCCTATTGCAGACATAATACTCATCATTATTTAGGATTCTTTCGATCAATCAGAGAAGCAATCAAGGCAGGCAAATTTCAGCAATATCATCAGAAATTCGTTGAGGGTAGGCGCAATCATCTAGCCCTGCCAGCCGCAGCAATCAACTCATGA

mRNA sequence

TGGGGAGGCGACTAGGGTTGAAGTGGCGTCCATATTTAAGAGCCCTCCACTATTTCAAACAGATTTTAGCGCCACACATCTGTGTCTGTTTTGAGGCAGAATGAAATTCGCGGTGAAGGCGTGGAGCAACTGCAAGGCGCGCACCGGATGTCTACAGCTCGGAAACTGCCCTACCGCCATTGATACTCCTGCTTTACTTCTCTCTACTTGTAAAGGACTGCCTAATTTCATCTCTCCTGATCTTCTTCCGTCTCTCCCTTCCCCTGATTCTCACCTCCTCCAGTTCAGTCCTCTTCACTTCTTGGAAGGTCCTTCATTGAAAACGATATCAAGTATTGGAGGGCTGCAGCATTTACTTGGGTTACATGAATATGTATTTGCAGCAGTAGCAAGGGATTCTGTTCAGTGCCTTCCAGAATGTAATGCTTCCAATAAATTTGGAGCATCTTTTGAGACTACTTGTGGTCGTCGTTTGATAAAACCTGTTGAGTATGTTGAATTGATTTCGTCCATGAGACCAGACATTTGGGCCTCCTTGGCTGATGAAGTGCCATCGTGGGTTTCAGATAAAAGGAATAAAACATCAGTGGATCGAACTGTGAGATGGCTCGATGAATGCATCAATTTGAACTCAACAGGTGGGGCTATTTTTGGAGCTATTGTTGGAGGCAATGATATGGAAGAACGCCAAAGATGTGCTTTAGAGGTGGTTAAGCGTAATGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATAGATGAGCGTCCTGCCCTACTTGATGCTGTTACGGATGTCTTGCCAGAGGAATTGCCACGTATGATTTGTGGGCTCGGACTTCCAGAGGAGATCTTGCAGGGTATTGCTGCTGGAGTCGATCTCTTTGACTCTGCGTACATATATCACTTGACCCTTGGAGGCTTTGCTCTCACCTTCCCACTGAATGGAATTGCGGAGAAACGGCCTGACACGCACCTGAACGACGATGCAAGTGATCGGACAAAAATCAACTTAAGAGCAACACTTTATCGGAAAGATACATCACCAATTCTCGAGGGGTGTAACTGCTACGCTTGCCTGAATCATACCAAGGCTTATATAAATCACTTGCTAAATGTCCACGAAATGCTGGCTCAAATACTGCTGGAGATACATAATACTCATCATTATTTAGGATTCTTTCGATCAATCAGAGAAGCAATCAAGGCAGGCAAATTTCAGCAATATCATCAGAAATTCGTTGAGGGTAGGCGCAATCATCTAGCCCTGCCAGCCGCAGCAATCAACTCATGA

Coding sequence (CDS)

ATGAAATTCGCGGTGAAGGCGTGGAGCAACTGCAAGGCGCGCACCGGATGTCTACAGCTCGGAAACTGCCCTACCGCCATTGATACTCCTGCTTTACTTCTCTCTACTTGTAAAGGACTGCCTAATTTCATCTCTCCTGATCTTCTTCCGTCTCTCCCTTCCCCTGATTCTCACCTCCTCCAGTTCAGTCCTCTTCACTTCTTGGAAGGTCCTTCATTGAAAACGATATCAAGTATTGGAGGGCTGCAGCATTTACTTGGGTTACATGAATATGTATTTGCAGCAGTAGCAAGGGATTCTGTTCAGTGCCTTCCAGAATGTAATGCTTCCAATAAATTTGGAGCATCTTTTGAGACTACTTGTGGTCGTCGTTTGATAAAACCTGTTGAGTATGTTGAATTGATTTCGTCCATGAGACCAGACATTTGGGCCTCCTTGGCTGATGAAGTGCCATCGTGGGTTTCAGATAAAAGGAATAAAACATCAGTGGATCGAACTGTGAGATGGCTCGATGAATGCATCAATTTGAACTCAACAGGTGGGGCTATTTTTGGAGCTATTGTTGGAGGCAATGATATGGAAGAACGCCAAAGATGTGCTTTAGAGGTGGTTAAGCGTAATGTATCAGGCTATTGGATTGGAGGTTTTGGGCTTGGAGAGAGCATAGATGAGCGTCCTGCCCTACTTGATGCTGTTACGGATGTCTTGCCAGAGGAATTGCCACGTATGATTTGTGGGCTCGGACTTCCAGAGGAGATCTTGCAGGGTATTGCTGCTGGAGTCGATCTCTTTGACTCTGCGTACATATATCACTTGACCCTTGGAGGCTTTGCTCTCACCTTCCCACTGAATGGAATTGCGGAGAAACGGCCTGACACGCACCTGAACGACGATGCAAGTGATCGGACAAAAATCAACTTAAGAGCAACACTTTATCGGAAAGATACATCACCAATTCTCGAGGGGTGTAACTGCTACGCTTGCCTGAATCATACCAAGGCTTATATAAATCACTTGCTAAATGTCCACGAAATGCTGGCTCAAATACTGCTGGAGATACATAATACTCATCATTATTTAGGATTCTTTCGATCAATCAGAGAAGCAATCAAGGCAGGCAAATTTCAGCAATATCATCAGAAATTCGTTGAGGGTAGGCGCAATCATCTAGCCCTGCCAGCCGCAGCAATCAACTCATGA

Protein sequence

MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLLQFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETTCGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTGGAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEELPRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDASDRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYLGFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAINS
Homology
BLAST of CmaCh04G030310 vs. ExPASy Swiss-Prot
Match: Q5R998 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Pongo abelii OX=9601 GN=QTRT2 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.9e-47
Identity = 119/327 (36.39%), Postives = 175/327 (53.52%), Query Frame = 0

Query: 81  GLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETTCGRRLIKPVEYVELISSMRP 140
           G+   +G+ E +      D V   P    +NK   S  +  GR  +   +++ +  +++P
Sbjct: 80  GVGKFIGMPESLLYCSLHDPVSPCPAGYVTNK-SVSVWSVAGRVEMTVSKFMAIQQALQP 139

Query: 141 DIWASLADEVPSW---VSDKRNKTSVDRTVRWLDECINLNSTG-----GAIFGAIVGGND 200
           D +  L+D   S     S KR + SVDR++ +LD C+ L           I G I GG+ 
Sbjct: 140 DWFQCLSDGEVSCKEATSIKRVRKSVDRSLLFLDNCLRLQEESEVLQKSVIIGVIEGGDV 199

Query: 201 MEERQRCALEVVKRNVSGYWIGGF-GLGESIDERPALLDAVTDVLPEELPRMICGLGLPE 260
           MEER R A E  KR V G+ + GF G   +++ R  LL +VT  LPE+ PR+I G+  P+
Sbjct: 200 MEERLRSARETAKRPVGGFLLDGFQGNPTTLETRLRLLSSVTAELPEDKPRLISGVSRPD 259

Query: 261 EILQGIAAGVDLFDSAYIYHLTLGGFALTFPL-------------NGIAE--------KR 320
           E+L+ I  GVDLF+S + Y +T  G ALTF               NG  E        K+
Sbjct: 260 EVLECIERGVDLFESFFPYQVTERGCALTFSFDYQPNPEETLLQQNGTQEEIKCMDQIKK 319

Query: 321 PDTHLNDDASDRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQIL 378
            +T   +      +INL+   Y++D +P++ GC+CY C NHT+AYI+HLL  +E+LA +L
Sbjct: 320 METTGCNQEITSFEINLKEKKYQEDFNPLVRGCSCYCCKNHTRAYIHHLLVTNELLAGVL 379

BLAST of CmaCh04G030310 vs. ExPASy Swiss-Prot
Match: Q7ZVJ6 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Danio rerio OX=7955 GN=qtrt2 PE=2 SV=2)

HSP 1 Score: 191.0 bits (484), Expect = 2.5e-47
Identity = 114/337 (33.83%), Postives = 179/337 (53.12%), Query Frame = 0

Query: 81  GLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETTCGRRLIKPVEYVELISSMRP 140
           G++   GLH+ V      DS    P  + +NK   S   + GR  +    ++ + ++++P
Sbjct: 80  GVRKFAGLHDTVIFCSLHDSASPSPAGHVTNK-TVSVWGSGGRIELTAARFMSIQAAVQP 139

Query: 141 DIWASLADEVPSW---VSDKRNKTSVDRTVRWLDECINLNS-----TGGAIFGAIVGGND 200
           D + S+AD   +W    S KR + +VDRT+  LDEC+ L+          IFG + GG+ 
Sbjct: 140 DCYQSMADG-ETWQANTSRKRVRKAVDRTLAHLDECLVLHQKTQELKHAEIFGVVEGGDI 199

Query: 201 MEERQRCALEVVKRNVSGYWIGGF-GLGESIDERPALLDAVTDVLPEELPRMICGLGLPE 260
           +EER R A E  KR V G+ + GF       D R  L+   +  LP+E PR++ G+G P+
Sbjct: 200 LEERLRSARETAKRPVGGFVLDGFHSSAMDQDVRAQLIQETSAELPQEKPRLVLGVGRPD 259

Query: 261 EILQGIAAGVDLFDSAYIYHLTLGGFALTF-------------------PLNGIAEKRPD 320
           E++  + AGVDLF+S + + +T  G AL+F                     NG   +   
Sbjct: 260 EVISCVEAGVDLFESFFPFQVTERGCALSFNYTIDPDPETAGTSASTVLECNGETPEVKK 319

Query: 321 THLNDDASDRT--KINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQIL 380
              N+D  + T  +INL+   YR D  P++EGC CY C  H +AY++HLL  +E+LA +L
Sbjct: 320 PSANEDVENMTPFEINLKDKRYRDDFRPLVEGCVCYCCQKHMRAYVHHLLVTNELLAGVL 379

Query: 381 LEIHNTHHYLGFFRSIREAIKAGKFQQYHQKFVEGRR 388
           L +HN  HYLGFF+++R+AI + + Q +    +  R+
Sbjct: 380 LMLHNMAHYLGFFKALRDAITSDRLQDFKNTVLHRRQ 414

BLAST of CmaCh04G030310 vs. ExPASy Swiss-Prot
Match: Q9H974 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Homo sapiens OX=9606 GN=QTRT2 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.5e-47
Identity = 119/327 (36.39%), Postives = 175/327 (53.52%), Query Frame = 0

Query: 81  GLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETTCGRRLIKPVEYVELISSMRP 140
           G+   +G+ E +      D V   P    +NK   S  +  GR  +   +++ +  +++P
Sbjct: 80  GVGKFIGMPESLLYCSLHDPVSPCPAGYVTNK-SVSVWSVAGRVEMTVSKFMAIQKALQP 139

Query: 141 DIWASLADEVPSW---VSDKRNKTSVDRTVRWLDECINLNSTG-----GAIFGAIVGGND 200
           D +  L+D   S     S KR + SVDR++ +LD C+ L           I G I GG+ 
Sbjct: 140 DWFQCLSDGEVSCKEATSIKRVRKSVDRSLLFLDNCLRLQEESEVLQKSVIIGVIEGGDV 199

Query: 201 MEERQRCALEVVKRNVSGYWIGGF-GLGESIDERPALLDAVTDVLPEELPRMICGLGLPE 260
           MEER R A E  KR V G+ + GF G   +++ R  LL +VT  LPE+ PR+I G+  P+
Sbjct: 200 MEERLRSARETAKRPVGGFLLDGFQGNPTTLEARLRLLSSVTAELPEDKPRLISGVSRPD 259

Query: 261 EILQGIAAGVDLFDSAYIYHLTLGGFALTFPL-------------NGIAE--------KR 320
           E+L+ I  GVDLF+S + Y +T  G ALTF               NG  E        K+
Sbjct: 260 EVLECIERGVDLFESFFPYQVTERGCALTFSFDYQPNPEETLLQQNGTQEEIKCMDQIKK 319

Query: 321 PDTHLNDDASDRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQIL 378
            +T   +      +INL+   Y++D +P++ GC+CY C NHT+AYI+HLL  +E+LA +L
Sbjct: 320 IETTGCNQEITSFEINLKEKKYQEDFNPLVRGCSCYCCKNHTRAYIHHLLVTNELLAGVL 379

BLAST of CmaCh04G030310 vs. ExPASy Swiss-Prot
Match: B8ZXI1 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Mus musculus OX=10090 GN=Qtrt2 PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 3.3e-47
Identity = 136/399 (34.09%), Postives = 198/399 (49.62%), Query Frame = 0

Query: 21  GNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLLQFSPLHFLEGPSLKTISSIG 80
           G+C   +D P  LL T  G    ++   L +             +H + G +  T+SS+ 
Sbjct: 24  GDC--TVDIPGCLLYTRTGSAPHLTHQTLRN-------------IHGVPGIAQLTLSSLA 83

Query: 81  -----------GLQHLLGLHEYVFAAVARDSVQCLPECNASNK------FGASFETTCGR 140
                      G+   +G+ E +F     D V   P    ++K      FG   E T  +
Sbjct: 84  EHHEVLAEYKKGVGSFIGMPESLFYCSLHDPVTPGPAGYVTSKSVSVWGFGGRVEMTVSK 143

Query: 141 RLIKPVEYVELISSMRPDIWASLADEVPS---WVSDKRNKTSVDRTVRWLDECINLNSTG 200
                  ++ +  +++PD +  L+D   S     S KR + SVDR++ +LD C+ L    
Sbjct: 144 -------FMAIQEALQPDWFQCLSDGEASCAETTSIKRARKSVDRSLLFLDSCLRLQEES 203

Query: 201 -----GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDE-RPALLDAVTD 260
                  I G I GG+ MEER R A E  KR V G+ + GF    ++ E R  LL +VT 
Sbjct: 204 EVLQKSVIIGVIEGGDVMEERLRSARETAKRPVGGFLLDGFQGDPAVTETRLHLLSSVTA 263

Query: 261 VLPEELPRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPL----------- 320
            LPE+ PR+ICG+  P+E+L+ I  GVDLF+S + Y +T  G ALTF             
Sbjct: 264 ELPEDKPRLICGVSRPDEVLECIERGVDLFESFFPYQVTERGCALTFTFDCQLNPEETLL 323

Query: 321 --NGI---------AEKRPDTHLNDDASDRTKINLRATLYRKDTSPILEGCNCYACLNHT 372
             NGI         A+K   T  N + +   +INL+   Y++D  P++ GC+CY C NHT
Sbjct: 324 QQNGIQEKIKGLDQAKKIEATGCNQEMTS-FEINLKEKKYQEDFDPLVRGCSCYCCKNHT 383

BLAST of CmaCh04G030310 vs. ExPASy Swiss-Prot
Match: Q6DF96 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Xenopus laevis OX=8355 GN=qtrt2 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 3.3e-47
Identity = 123/326 (37.73%), Postives = 174/326 (53.37%), Query Frame = 0

Query: 81  GLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETTCGRRLIKPVEYVELISSMRP 140
           G+    GL + VF     D V   P    +NK   S   + GR  +   +++     +RP
Sbjct: 80  GIGKFAGLPDAVFYCSTHDPVSPCPTGYNTNK-AVSLWGSGGRIEMTTQKFISAQRVLRP 139

Query: 141 DIWASLAD--EVPSWVSDKRNKTSVDRTVRWLDECINLNSTGG-----AIFGAIVGGNDM 200
           D +  L+D    P   S KR K SVDR++ +LDEC+ L S         + GA+ GG+ +
Sbjct: 140 DWFQCLSDGEVTPGGNSRKRIKKSVDRSLVFLDECLQLLSEHEELKPCVLIGAVEGGDLL 199

Query: 201 EERQRCALEVVKRNVSGYWIGGF-GLGESIDERPALLDAVTDVLPEELPRMICGLGLPEE 260
           +ER R A E  KR V G+ + GF G+    + + +L+ AVT  LPE+ PR I G+G P+E
Sbjct: 200 DERLRSARETAKRPVGGFLLDGFHGISAGNEAKLSLVSAVTAELPEDKPRFIHGVGRPDE 259

Query: 261 ILQGIAAGVDLFDSAYIYHLTLGGFALTF-------PLNGIAEK--RPDTHLNDDA---- 320
           +L+ I  GVDLFDS + Y +T  G AL F       P   + EK    DT  N D     
Sbjct: 260 VLEFIQRGVDLFDSCFPYQVTERGCALIFTHCHRPDPETAVLEKSEMSDTERNGDVGAEI 319

Query: 321 ----SDRT-----KINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQIL 377
               +D+      +I L+   +R+D  P+LEGC CY C NH++AY++HLL   E+LA IL
Sbjct: 320 EEPDADQAEMTPFEICLKEKRFREDFGPLLEGCTCYCCRNHSRAYVHHLLMAKELLAGIL 379

BLAST of CmaCh04G030310 vs. ExPASy TrEMBL
Match: A0A6J1JNK0 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111487476 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 6.3e-235
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT
Sbjct: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG
Sbjct: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL
Sbjct: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360
           DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL
Sbjct: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360

Query: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAINS 400
           GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAINS
Sbjct: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAINS 399

BLAST of CmaCh04G030310 vs. ExPASy TrEMBL
Match: A0A6J1HIG1 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucurbita moschata OX=3662 GN=LOC111464336 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 8.5e-232
Identity = 393/397 (98.99%), Postives = 395/397 (99.50%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAWSNCKARTGC+QLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNCKARTGCIQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPSLK ISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT
Sbjct: 61  QFSPLHFLEGPSLKAISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLI+PVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECI LNSTG
Sbjct: 121 CGRRLIRPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECIKLNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL
Sbjct: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360
           DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL
Sbjct: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360

Query: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI 398
           GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI
Sbjct: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI 397

BLAST of CmaCh04G030310 vs. ExPASy TrEMBL
Match: A0A6J1DE50 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Momordica charantia OX=3673 GN=LOC111020219 PE=3 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 3.4e-212
Identity = 360/399 (90.23%), Postives = 379/399 (94.99%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAWSN KARTG LQLGNCPTAI+TPALLLST KGL NFISPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNGKARTGVLQLGNCPTAIETPALLLSTRKGLLNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPS KTISSIGGLQHLLGLHEY FAAVARDSV CLPEC+A+NKFGASFETT
Sbjct: 61  QFSPLHFLEGPSSKTISSIGGLQHLLGLHEYGFAAVARDSVWCLPECDAANKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLI+PVEY+E+ISS+RPDIWASLADEVPSWVSDKRNKTSVDRTV+WLDECI  NSTG
Sbjct: 121 CGRRLIRPVEYIEMISSLRPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECITSNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GA+FGAIVGGND+EERQRCA EV+KRNVSGYWIGGFGLGES+DERPALLDAVT +LPEE+
Sbjct: 181 GAVFGAIVGGNDVEERQRCAAEVIKRNVSGYWIGGFGLGESMDERPALLDAVTGILPEEM 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLT+GGFALTFPLNGI EK PD HLND+ S
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTIGGFALTFPLNGIVEKVPDLHLNDNGS 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360
           D TKINLRATLYRKDTSPILEGC+CY CLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL
Sbjct: 301 DLTKINLRATLYRKDTSPILEGCDCYTCLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360

Query: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAINS 400
           GFFRSIREAI+AGKFQQYHQKFVE RRNHLA PA AI+S
Sbjct: 361 GFFRSIREAIEAGKFQQYHQKFVEVRRNHLAFPALAISS 399

BLAST of CmaCh04G030310 vs. ExPASy TrEMBL
Match: A0A1S3B3I7 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucumis melo OX=3656 GN=LOC103485379 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 3.7e-211
Identity = 355/398 (89.20%), Postives = 378/398 (94.97%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAWSN KARTG LQLGNCP AIDTPALLLST KGLPNFISPDLLPSLPSPDSH+L
Sbjct: 1   MKFAVKAWSNGKARTGFLQLGNCPAAIDTPALLLSTSKGLPNFISPDLLPSLPSPDSHIL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPS KTISSIGGLQHLLGLHEY FAAVARDSVQCLP+C+A+NKFGASFETT
Sbjct: 61  QFSPLHFLEGPSSKTISSIGGLQHLLGLHEYGFAAVARDSVQCLPQCDATNKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLI+PVEY+E+ISSM+PDIWASLADEVPSWVSDKRNKTSVDRTV+WLDECI LNSTG
Sbjct: 121 CGRRLIRPVEYIEMISSMKPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECITLNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GA+FGAIVGGND+EERQRCA+EVVKRNVSGYWIGGFG GES+DERP+LLDAVTDVLPEE+
Sbjct: 181 GAVFGAIVGGNDLEERQRCAVEVVKRNVSGYWIGGFGFGESMDERPSLLDAVTDVLPEEM 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPL+GI EK+P  HL D+A 
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLDGIVEKQPAAHLIDNAI 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360
           D TKINLRAT+YRKDTSPILEGC CY CLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL
Sbjct: 301 DMTKINLRATIYRKDTSPILEGCTCYTCLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360

Query: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAIN 399
           GFFRSIREAIK GKFQQ+HQKFVE RRNHL LP +A++
Sbjct: 361 GFFRSIREAIKVGKFQQFHQKFVEERRNHLTLPKSALS 398

BLAST of CmaCh04G030310 vs. ExPASy TrEMBL
Match: A0A5A7T128 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00430 PE=3 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.9e-187
Identity = 317/354 (89.55%), Postives = 338/354 (95.48%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAWSN KARTG LQLGNCP AIDTPALLLST KGLPNFISPDLLPSLPSPDSH+L
Sbjct: 1   MKFAVKAWSNGKARTGFLQLGNCPAAIDTPALLLSTSKGLPNFISPDLLPSLPSPDSHIL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPS KTISSIGGLQHLLGLHEY FAAVARDSVQCLP+C+A+NKFGASFETT
Sbjct: 61  QFSPLHFLEGPSSKTISSIGGLQHLLGLHEYGFAAVARDSVQCLPQCDATNKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLI+PVEY+E+ISSM+PDIWASLADEVPSWVSDKRNKTSVDRTV+WLDECI LNSTG
Sbjct: 121 CGRRLIRPVEYIEMISSMKPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECITLNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GA+FGAIVGGND+EERQRCA+EVVKRNVSGYWIGGFG GES+DERP+LLDAVTDVLPEE+
Sbjct: 181 GAVFGAIVGGNDLEERQRCAVEVVKRNVSGYWIGGFGFGESMDERPSLLDAVTDVLPEEM 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPL+GI EK+P  HL D+A 
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLDGIVEKQPAAHLIDNAI 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIH 355
           D TKINLRAT+YRKDTSPILEGC CY CLNHTKAYINHLLNVHEMLAQILLE++
Sbjct: 301 DMTKINLRATIYRKDTSPILEGCTCYTCLNHTKAYINHLLNVHEMLAQILLEMY 354

BLAST of CmaCh04G030310 vs. NCBI nr
Match: XP_022990656.1 (queuine tRNA-ribosyltransferase accessory subunit 2-like [Cucurbita maxima])

HSP 1 Score: 822.8 bits (2124), Expect = 1.3e-234
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT
Sbjct: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG
Sbjct: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL
Sbjct: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360
           DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL
Sbjct: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360

Query: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAINS 400
           GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAINS
Sbjct: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAINS 399

BLAST of CmaCh04G030310 vs. NCBI nr
Match: KAG6602735.1 (Queuine tRNA-ribosyltransferase accessory subunit 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 815.1 bits (2104), Expect = 2.7e-232
Identity = 395/397 (99.50%), Postives = 396/397 (99.75%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT
Sbjct: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLI+PVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECI LNSTG
Sbjct: 121 CGRRLIRPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECIKLNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL
Sbjct: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360
           DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL
Sbjct: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360

Query: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI 398
           GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI
Sbjct: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI 397

BLAST of CmaCh04G030310 vs. NCBI nr
Match: XP_022964271.1 (queuine tRNA-ribosyltransferase accessory subunit 2-like [Cucurbita moschata])

HSP 1 Score: 812.4 bits (2097), Expect = 1.8e-231
Identity = 393/397 (98.99%), Postives = 395/397 (99.50%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAWSNCKARTGC+QLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNCKARTGCIQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPSLK ISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT
Sbjct: 61  QFSPLHFLEGPSLKAISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLI+PVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECI LNSTG
Sbjct: 121 CGRRLIRPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECIKLNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL
Sbjct: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360
           DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL
Sbjct: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360

Query: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI 398
           GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI
Sbjct: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI 397

BLAST of CmaCh04G030310 vs. NCBI nr
Match: KAG7033422.1 (Queuine tRNA-ribosyltransferase accessory subunit 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 809.3 bits (2089), Expect = 1.5e-230
Identity = 393/397 (98.99%), Postives = 395/397 (99.50%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT
Sbjct: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLI+PVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECI LNSTG
Sbjct: 121 CGRRLIRPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECIKLNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL
Sbjct: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYI HLTLGGFALTFPLNGIAEKRPDTHLNDDAS
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYICHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360
           DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL
Sbjct: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360

Query: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI 398
           GFFRSIREAIKAGKFQQY+QKFVEGRRNHLALPAAAI
Sbjct: 361 GFFRSIREAIKAGKFQQYYQKFVEGRRNHLALPAAAI 397

BLAST of CmaCh04G030310 vs. NCBI nr
Match: XP_023511781.1 (queuine tRNA-ribosyltransferase accessory subunit 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023511793.1 queuine tRNA-ribosyltransferase accessory subunit 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 809.3 bits (2089), Expect = 1.5e-230
Identity = 392/397 (98.74%), Postives = 395/397 (99.50%), Query Frame = 0

Query: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60
           MKFAVKAW+NCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWNNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120
           QFSPLHFLEGPSLKTISSIGGLQHLLGLHE VFAAVARDSVQCLPECNASNKFGASFETT
Sbjct: 61  QFSPLHFLEGPSLKTISSIGGLQHLLGLHESVFAAVARDSVQCLPECNASNKFGASFETT 120

Query: 121 CGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180
           CGRRLI+PVEYVELISSMRP+IWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG
Sbjct: 121 CGRRLIRPVEYVELISSMRPNIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINLNSTG 180

Query: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240
           GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL
Sbjct: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240

Query: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300
           PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDD S
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDVS 300

Query: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360
           DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL
Sbjct: 301 DRTKINLRATLYRKDTSPILEGCNCYACLNHTKAYINHLLNVHEMLAQILLEIHNTHHYL 360

Query: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI 398
           GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI
Sbjct: 361 GFFRSIREAIKAGKFQQYHQKFVEGRRNHLALPAAAI 397

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5R9981.9e-4736.39Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Pongo abelii OX=9601 GN=Q... [more]
Q7ZVJ62.5e-4733.83Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Danio rerio OX=7955 GN=qt... [more]
Q9H9742.5e-4736.39Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Homo sapiens OX=9606 GN=Q... [more]
B8ZXI13.3e-4734.09Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Mus musculus OX=10090 GN=... [more]
Q6DF963.3e-4737.73Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Xenopus laevis OX=8355 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1JNK06.3e-235100.00Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1HIG18.5e-23298.99Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucurbita moschata OX=366... [more]
A0A6J1DE503.4e-21290.23Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Momordica charantia OX=36... [more]
A0A1S3B3I73.7e-21189.20Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucumis melo OX=3656 GN=L... [more]
A0A5A7T1282.9e-18789.55Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucumis melo var. makuwa ... [more]
Match NameE-valueIdentityDescription
XP_022990656.11.3e-234100.00queuine tRNA-ribosyltransferase accessory subunit 2-like [Cucurbita maxima][more]
KAG6602735.12.7e-23299.50Queuine tRNA-ribosyltransferase accessory subunit 2, partial [Cucurbita argyrosp... [more]
XP_022964271.11.8e-23198.99queuine tRNA-ribosyltransferase accessory subunit 2-like [Cucurbita moschata][more]
KAG7033422.11.5e-23098.99Queuine tRNA-ribosyltransferase accessory subunit 2 [Cucurbita argyrosperma subs... [more]
XP_023511781.11.5e-23098.74queuine tRNA-ribosyltransferase accessory subunit 2-like isoform X1 [Cucurbita p... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036511Queuine tRNA-ribosyltransferase-likeGENE3D3.20.20.105coord: 1..387
e-value: 5.0E-110
score: 369.8
IPR036511Queuine tRNA-ribosyltransferase-likeSUPERFAMILY51713tRNA-guanine transglycosylasecoord: 1..384
IPR002616tRNA-guanine(15) transglycosylase-likeTIGRFAMTIGR00449TIGR00449coord: 13..385
e-value: 7.2E-48
score: 161.2
IPR002616tRNA-guanine(15) transglycosylase-likePFAMPF01702TGTcoord: 11..386
e-value: 2.0E-97
score: 326.5
NoneNo IPR availablePANTHERPTHR46064QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2coord: 1..390
IPR028592Queuine tRNA-ribosyltransferase accessory subunit QTRTD1HAMAPMF_03043QTRT2coord: 25..381
score: 33.84988

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G030310.1CmaCh04G030310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0101030 tRNA-guanine transglycosylation
biological_process GO:0006400 tRNA modification
cellular_component GO:0005737 cytoplasm
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008479 queuine tRNA-ribosyltransferase activity
molecular_function GO:0016763 pentosyltransferase activity