CmaCh04G030260 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh04G030260 vs. ExPASy Swiss-Prot
Match: Q8W5R2 (Phospholipase SGR2 OS=Arabidopsis thaliana OX=3702 GN=SGR2 PE=1 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 9.7e-134
Identity = 332/988 (33.60%), Postives = 420/988 (42.51%), Query Frame = 0

Query: 48  IGSSEVSEASPDSLKNTPSNIAKLEDVIEHC----------------------------- 107
           +G+ EV+E SPD LKNTPSNIA+LEDVIE C                             
Sbjct: 8   LGTREVNETSPDLLKNTPSNIARLEDVIEQCHGRQKYLAQTRSPSDGSDVRWYFCKVPLA 67

Query: 108 -EELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVRMEGRKFQKFLAKGRKVRYD 167
             ELAASVP+T++VGK EYFRFGMRDSLAIEASFLQ                        
Sbjct: 68  ENELAASVPRTDVVGKSEYFRFGMRDSLAIEASFLQ------------------------ 127

Query: 168 SWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWKEIAECSEGPKERAGTGLRL 227
                                         RE+ELLS+WWKE AECSEGPK +  +    
Sbjct: 128 ------------------------------REDELLSLWWKEYAECSEGPKLQVNS---- 187

Query: 228 DKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKRHCFPVYWNAENRRVMRGHW 287
            K+  E+ SE +    LY++EEERVGV VKGGLYEVDLV+RHCFPVYWN +NRRV+RGHW
Sbjct: 188 KKKSIETPSEASVSSSLYEVEEERVGVPVKGGLYEVDLVRRHCFPVYWNGDNRRVLRGHW 247

Query: 288 FACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAPENISAIRTFCILRSTPGLH 347
           FA KGGLDWLP+ E V+EQLE+AY   ++  +  F     P  + A R   +  S+ GLH
Sbjct: 248 FARKGGLDWLPIPETVSEQLEVAY-RNKVWRRRSFQ----PSGLFAAR-IDLQGSSLGLH 307

Query: 348 ALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLSNAPKPTQDDLRQQREEEMD 407
           ALFTGEDDTWEAWLNVD SGFS ++   GNGIKLRRGY+ S +PKPTQ++LRQQ+EEEMD
Sbjct: 308 ALFTGEDDTWEAWLNVDPSGFSGIVGYTGNGIKLRRGYAGSYSPKPTQEELRQQKEEEMD 367

Query: 408 DY----------FSIH-------------------------------------------- 467
           DY          F +H                                            
Sbjct: 368 DYCSQVPVRHLVFMVHGIGQKGEKSNLVDDVGNFRQITAALAERHLTSHQLSTQRVLFIP 427

Query: 468 -----------------------------------------------FVID--------- 527
                                                           +ID         
Sbjct: 428 CQWRKGLKLSGEAAVDKCTLDGVRRFREMLSATVHDVLYYMSPIYCQAIIDSVSKQLNRL 487

Query: 528 -------------------------IQYDL---------------------PD------- 587
                                    + YD+                     PD       
Sbjct: 488 YLKFLKRNPDYVGKISIYGHSLGSVLSYDILCHQHNLSSPFPMDSVYKKFFPDEESPPTP 547

Query: 588 --------SGPHDFYDPK------------------------------------------ 637
                   S P   ++P+                                          
Sbjct: 548 AKADKPCSSHPSSNFEPEKSDQLNNPEKITGQDNNTMAKEPTVLEHHDVIQEDPSLISDS 607

BLAST of CmaCh04G030260 vs. ExPASy Swiss-Prot
Match: Q9Y6Y8 (SEC23-interacting protein OS=Homo sapiens OX=9606 GN=SEC23IP PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.8e-18
Identity = 70/235 (29.79%), Postives = 115/235 (48.94%), Query Frame = 0

Query: 386 IDIQYDLPDSGPHDFY---DPKSLFVWSSGKRKEYWDEENISEEMPACRQMLNIFHPFDP 445
           + + Y+  D  P  F+    P ++F+   G  +    +EN S  +P C+   NI+HP DP
Sbjct: 772 VSVAYNSLDFEPEIFFALGSPIAMFLTIRGVDR---IDENYS--LPTCKGFFNIYHPLDP 831

Query: 446 VAYRIEPLVCKEYMLKRPVIIPFHRGGRRLHIGFREFTDNLALRSQAKKDDLHTLGVKVL 505
           VAYR+EP++  +  LK  V+IP H+G +RLH+  +E    +   S  K+  + +L     
Sbjct: 832 VAYRLEPMIVPDLDLK-AVLIPHHKGRKRLHLELKESLSRMG--SDLKQGFISSLKSAWQ 891

Query: 506 TVCQSKQA----DGLEEGDENFQGELK---CYGVAMMERLTGSE---------------- 565
           T+ +  +A      L+E  E    ++K      V   E++  S                 
Sbjct: 892 TLNEFARAHTSSTQLQEELEKVANQIKEEEEKQVVEAEKVVESPDFSKDEDYLGKVGMLN 951

Query: 566 -GGRIDHMLQDKTCE--HPYMQAIKSHTNYWRDHDTAIFILKHLYRDILEDPDTP 592
            G RID++LQ+K  E  + Y+ A++SH  YW   DTA+ +LK +YR +   P+ P
Sbjct: 952 GGRRIDYVLQEKPIESFNEYLFALQSHLCYWESEDTALLLLKEIYRTMNISPEQP 998

BLAST of CmaCh04G030260 vs. ExPASy Swiss-Prot
Match: Q6NZC7 (SEC23-interacting protein OS=Mus musculus OX=10090 GN=Sec23ip PE=1 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 2.5e-17
Identity = 59/191 (30.89%), Postives = 97/191 (50.79%), Query Frame = 0

Query: 427 MPACRQMLNIFHPFDPVAYRIEPLVCKEYMLKRPVIIPFHRGGRRLHIGFREFTDNLALR 486
           +P C+   NI+HP DPVAYR+EP++  +  LK  V++P H+G +RLH+  +E    +   
Sbjct: 809 LPTCKGFFNIYHPLDPVAYRLEPMIAPDLDLK-AVLVPHHKGRKRLHLELKESLSRMG-- 868

Query: 487 SQAKKDDLHTLGVKVLTVCQ---------------SKQADGLEEGDENFQGELK----CY 546
           S  K+  + +L     T+ +                K A+ ++E +E    E K      
Sbjct: 869 SDLKQGFISSLKSAWQTLNEFARAHTSTTQLQEELEKVANQIKEEEEKQVVEAKKKKESP 928

Query: 547 GVAMMERLTG-----SEGGRIDHMLQDKTCE--HPYMQAIKSHTNYWRDHDTAIFILKHL 592
            ++  E   G     + G RID++LQ+K  E  + Y+ A++SH  YW   DTA+ +LK +
Sbjct: 929 ELSKDEDYLGKVGMLNGGRRIDYVLQEKPIESFNEYLFALQSHLCYWGSEDTALLLLKEI 988

BLAST of CmaCh04G030260 vs. ExPASy Swiss-Prot
Match: Q80Y98 (Phospholipase DDHD2 OS=Mus musculus OX=10090 GN=Ddhd2 PE=1 SV=3)

HSP 1 Score: 90.9 bits (224), Expect = 5.7e-17
Identity = 66/260 (25.38%), Postives = 120/260 (46.15%), Query Frame = 0

Query: 347 NGIKLRRGYSLSNAPKPTQDDLRQQREEEMDDYFSIHF-VIDIQYDLPDSGPHDFY---D 406
           N    R+     N P  +  ++   +E    DY  +    + ++Y   +  P  F+    
Sbjct: 439 NHFSARKNSVSINRPAMSASEVNISKEN--GDYLDVGIGQVSVKYPRLNYKPEIFFAFGS 498

Query: 407 PKSLFVWSSGKRKEYWDEENISEEMPACRQMLNIFHPFDPVAYRIEPLVCKEYMLKRPVI 466
           P  +F+   G R+      + + + P C+   NI+HPFDPVAYRIEP+V      + P++
Sbjct: 499 PIGMFLTVRGLRR-----IDPNYKFPTCKGFFNIYHPFDPVAYRIEPMVAPGIEFE-PML 558

Query: 467 IPFHRGGRRLHIGFREFTDNLALRSQAKKDDLHTL-------------GVKVLTVCQSKQ 526
           IP H+G +R+H+  RE    +++    K + L +L              ++     +  +
Sbjct: 559 IPHHKGRKRMHLELREGLTRMSM--DLKNNLLGSLRMAWKSFTRGPYPALQASETAEETE 618

Query: 527 ADGLEEGDENFQGELKCYGVAMMERLTGS-----EGGRIDHMLQDKTCE--HPYMQAIKS 583
           A+     +++ +   +   V + E    S      G RID++LQ+K  E  + Y+ A++S
Sbjct: 619 AEPESSSEKSNEANTEEPPVEVKEEAPISVGMLNGGQRIDYVLQEKPIESFNEYLFALQS 678

BLAST of CmaCh04G030260 vs. ExPASy Swiss-Prot
Match: O94830 (Phospholipase DDHD2 OS=Homo sapiens OX=9606 GN=DDHD2 PE=1 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 2.8e-16
Identity = 66/260 (25.38%), Postives = 116/260 (44.62%), Query Frame = 0

Query: 348 GIKLRRGYSLSNAPKPTQDDLRQQREEEMDDYFSIHF-VIDIQYDLPDSGPHDFY---DP 407
           GIK       S A  P + +          DY  +    + ++Y      P  F+    P
Sbjct: 449 GIKRPAPQPASGANIPKESEFCSSSNTRNGDYLDVGIGQVSVKYPRLIYKPEIFFAFGSP 508

Query: 408 KSLFVWSSGKRKEYWDEENISEEMPACRQMLNIFHPFDPVAYRIEPLVCKEYMLKRPVII 467
             +F+   G ++      + +   P C+   NI+HPFDPVAYRIEP+V      + P++I
Sbjct: 509 IGMFLTVRGLKR-----IDPNYRFPTCKGFFNIYHPFDPVAYRIEPMVVPGVEFE-PMLI 568

Query: 468 PFHRGGRRLHIGFREFTDNLALRSQAKKDDLHTL-------------GVKVLTVCQSKQA 527
           P H+G +R+H+  RE    +++    K + L +L              ++     +  +A
Sbjct: 569 PHHKGRKRMHLELREGLTRMSM--DLKNNLLGSLRMAWKSFTRAPYPALQASETPEETEA 628

Query: 528 DGLEEGDENFQGELKCYGVAMMERLTG------SEGGRIDHMLQDKTCE--HPYMQAIKS 583
           +     ++      +   VA+ E +        + G RID++LQ+K  E  + Y+ A++S
Sbjct: 629 EPESTSEKPSDVNTEETSVAVKEEVLPINVGMLNGGQRIDYVLQEKPIESFNEYLFALQS 688

BLAST of CmaCh04G030260 vs. ExPASy TrEMBL
Match: A0A6J1JU90 (LOW QUALITY PROTEIN: phospholipase SGR2-like OS=Cucurbita maxima OX=3661 GN=LOC111487550 PE=4 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 2.5e-286
Identity = 515/657 (78.39%), Postives = 519/657 (79.00%), Query Frame = 0

Query: 29  MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCE--------- 88
           MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCE         
Sbjct: 1   MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCEGRQKYLAQT 60

Query: 89  ---------------------ELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVR 148
                                ELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQ     
Sbjct: 61  RSPSDGGDVRWYFCKVPLLGTELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQ----- 120

Query: 149 MEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWK 208
                                                            REEELLSIWWK
Sbjct: 121 -------------------------------------------------REEELLSIWWK 180

Query: 209 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 268
           EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR
Sbjct: 181 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 240

Query: 269 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAP 328
           HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSR    +     G+  
Sbjct: 241 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRVWRRRTFQPSGLFA 300

Query: 329 ENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 388
             +       +  STPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS
Sbjct: 301 SXVD------LQGSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 360

Query: 389 NAPKPTQDDLRQQREEEMDDYFSIHFVIDIQYDLPDSGPHDFYDPKSLFVWSSGKRKEYW 448
           NAPKPTQDDLRQQREEEMDDY               S PHDFYDPKSLFVWSSGKRKEYW
Sbjct: 361 NAPKPTQDDLRQQREEEMDDYC--------------SQPHDFYDPKSLFVWSSGKRKEYW 420

Query: 449 DEENISEEMPACRQMLNIFHPFDPVA----------------YRIEPLVCKEYMLKRPVI 508
           DEENISEEMPACRQMLNIFHPFDPVA                YRIEPLVCKEYMLKRPVI
Sbjct: 421 DEENISEEMPACRQMLNIFHPFDPVAYSIPYGERIMEALLDLYRIEPLVCKEYMLKRPVI 480

Query: 509 IPFHRGGRRLHIGFREFTDNLALRSQAKKDDLHTLGVKVLTVCQSKQADGLEEGDENFQG 568
           IPFHRGGRRLHIGFREFTDNLALRSQAKKDDLHTLGVKVLTVCQSKQADGLEEGDENFQG
Sbjct: 481 IPFHRGGRRLHIGFREFTDNLALRSQAKKDDLHTLGVKVLTVCQSKQADGLEEGDENFQG 540

Query: 569 ELKCYGVAMMERLTGSEGGRIDHMLQDKTCEHPYMQAIKSHTNYWRDHDTAIFILKHLYR 628
           ELKCYGVAMMERLTGSEGGRIDHMLQDKTCEHPYMQAIKSHTNYWRDHDTAIFILKHLYR
Sbjct: 541 ELKCYGVAMMERLTGSEGGRIDHMLQDKTCEHPYMQAIKSHTNYWRDHDTAIFILKHLYR 583

Query: 629 DILEDPDTPPEYTEPDSNDCWYNRRETIEEELSLTFSNENVVRNFSRKAKKMIKNHR 640
           DILEDPDTPPEYTEPDSNDCWYNRRETIEEELSLTFSNENVVRNFSRKAKKMIKNHR
Sbjct: 601 DILEDPDTPPEYTEPDSNDCWYNRRETIEEELSLTFSNENVVRNFSRKAKKMIKNHR 583

BLAST of CmaCh04G030260 vs. ExPASy TrEMBL
Match: A0A6J1DHQ8 (phospholipase SGR2 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111020169 PE=4 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 2.6e-206
Identity = 458/983 (46.59%), Postives = 496/983 (50.46%), Query Frame = 0

Query: 29  MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCE--------- 88
           MD NS++D+  GDML+ +AIGSSEV EASPDSLKNTPSNIAKLEDVIEHCE         
Sbjct: 1   MDANSTEDIGGGDMLTSSAIGSSEVPEASPDSLKNTPSNIAKLEDVIEHCEGRQKYLAHT 60

Query: 89  ---------------------ELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVR 148
                                ELAASVPKTEIVGKG+YFRFGMRDSLAIEASFL      
Sbjct: 61  RSPSDGGDVRWYFCKVPLLGTELAASVPKTEIVGKGDYFRFGMRDSLAIEASFL------ 120

Query: 149 MEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWK 208
                                                           KREEELLSIWWK
Sbjct: 121 ------------------------------------------------KREEELLSIWWK 180

Query: 209 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 268
           E AECSEGPKER+G+  RLDKQR+ESASEVAKL+KLYDIEEERVGV VKGGLYEVDLVKR
Sbjct: 181 EYAECSEGPKERSGSSQRLDKQRHESASEVAKLVKLYDIEEERVGVPVKGGLYEVDLVKR 240

Query: 269 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAP 328
           HCFPVYWNAENRRVMRGHWFA KGGLDWLP+REDVAEQLEIAY SR +  +  F     P
Sbjct: 241 HCFPVYWNAENRRVMRGHWFARKGGLDWLPIREDVAEQLEIAYRSR-VWRRRAFQ----P 300

Query: 329 ENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 388
             + A R   +  STPGLHALFTGED+TWEAWLNVDASGFSSVI+LGGNGIKLRRGYS S
Sbjct: 301 SGLFASRV-DLQGSTPGLHALFTGEDNTWEAWLNVDASGFSSVIALGGNGIKLRRGYSPS 360

Query: 389 NAPKPTQDDLRQQREEEMDDY----------FSIH------------------------- 448
           N+PKPTQDDLRQQ+EEEMDDY          F +H                         
Sbjct: 361 NSPKPTQDDLRQQKEEEMDDYCSQVPVRHLVFMVHGIGQRLEKSNLVDDVGNFRHITSSL 420

Query: 449 ------------------------------------------------------------ 508
                                                                       
Sbjct: 421 GERHLTLHQRSTQRVLFIPCQWRKGLKLSGEAAVEKITLDGVKGLRVMLGATAHDVLYYM 480

Query: 509 ---------------------------------------FVIDIQYDLPD---------- 568
                                                  F +D  Y  P           
Sbjct: 481 SPIYCQDIINSVSNQLNRLYMKFLRRNPGYDGKENLSSPFPMDWMYAEPTRSEGFSGVDN 540

Query: 569 -------------------------------------------SGPHDFYDP-------- 628
                                                        P  F DP        
Sbjct: 541 KSSTRNSSCSTEDKSSTAAYGTADIVHFTKEDERNVHEMHLHLEDPSIFLDPVTSNSAEL 600

Query: 629 -----------------------------------------------------------K 640
                                                                      K
Sbjct: 601 ITTHENTSGGTEYDSSKRLPHTSDGLEEFKNWNCELEVPSVNKVAELQFEDSIDKDEAIK 660

BLAST of CmaCh04G030260 vs. ExPASy TrEMBL
Match: A0A6J1FF94 (phospholipase SGR2 OS=Cucurbita moschata OX=3662 GN=LOC111444941 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 4.9e-205
Identity = 462/1002 (46.11%), Postives = 495/1002 (49.40%), Query Frame = 0

Query: 29  MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHC---------- 88
           MDVNSSK+M   DML  AAIGSS V EASPDSLKNTPSNIAKLEDVIEHC          
Sbjct: 1   MDVNSSKNMGDDDMLISAAIGSSVVPEASPDSLKNTPSNIAKLEDVIEHCVGRQKYLAQT 60

Query: 89  --------------------EELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVR 148
                                E+AASVPKTEIVGKG+Y RFGMRDSLAIEASFLQ     
Sbjct: 61  RSPSDGGDVRWYFCKVPLLGNEVAASVPKTEIVGKGDYLRFGMRDSLAIEASFLQ----- 120

Query: 149 MEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWK 208
                                                            REEELLSIWWK
Sbjct: 121 -------------------------------------------------REEELLSIWWK 180

Query: 209 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 268
           E AECSEGPKERAG+ L++DKQR ESASEVAKL KLYDIEEERVGV VKGGLYEVDLVKR
Sbjct: 181 EYAECSEGPKERAGSSLKIDKQRNESASEVAKLAKLYDIEEERVGVPVKGGLYEVDLVKR 240

Query: 269 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAP 328
           HCFPVYWNAENRRVMRGHWFA KGGLDWLPLREDV EQLEIAY S Q+  +  F     P
Sbjct: 241 HCFPVYWNAENRRVMRGHWFARKGGLDWLPLREDVGEQLEIAYRS-QVWHRRTFQ----P 300

Query: 329 ENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 388
             + A R   +  STPGLHALFTGEDDTWEAWLNVD SGFSSVISLGGNGIKLRRGYS S
Sbjct: 301 SGLFASRV-DLQGSTPGLHALFTGEDDTWEAWLNVDTSGFSSVISLGGNGIKLRRGYSPS 360

Query: 389 NAPKPTQDDLRQQREEEMDDY----------FSIHFV---------ID------------ 448
           N+PKPTQDDLRQQREEEMDDY          F +H +         +D            
Sbjct: 361 NSPKPTQDDLRQQREEEMDDYCSQVPVRHLVFMVHGIGQRLEKSNLVDDVGNFRHITSSL 420

Query: 449 ------------------------------------------------------------ 508
                                                                       
Sbjct: 421 GERHLTLHQRSTQRVLFIPCQWRKGLKLSGEAAVEKITLDGVKGLRVMLGATAHDVLYYM 480

Query: 509 --------------------------------------------IQYDL----------- 568
                                                       + YD+           
Sbjct: 481 SPIYCQDIINSVSNQLNRLYMKFLRRNPGYDGKVSIYGHSLGSVLSYDILCHQKNLSSPF 540

Query: 569 -------------PDSGPHD--------------------------------------FY 628
                          SG HD                                        
Sbjct: 541 PMDWMYGEHARSEGFSGVHDQSSAQNSSCNTENNCSTAVYGCADIVQLAKEDGERNMHLE 600

Query: 629 DP---------------------------------------------------------- 640
           DP                                                          
Sbjct: 601 DPSIVADPVPLHSSDLIIKHENACGEADYDSSKRLPQTSDELEELNKNENCDLEVPSLNR 660

BLAST of CmaCh04G030260 vs. ExPASy TrEMBL
Match: A0A6J1DDX7 (phospholipase SGR2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020169 PE=4 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 8.3e-205
Identity = 458/996 (45.98%), Postives = 496/996 (49.80%), Query Frame = 0

Query: 29  MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCE--------- 88
           MD NS++D+  GDML+ +AIGSSEV EASPDSLKNTPSNIAKLEDVIEHCE         
Sbjct: 1   MDANSTEDIGGGDMLTSSAIGSSEVPEASPDSLKNTPSNIAKLEDVIEHCEGRQKYLAHT 60

Query: 89  ---------------------ELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVR 148
                                ELAASVPKTEIVGKG+YFRFGMRDSLAIEASFL      
Sbjct: 61  RSPSDGGDVRWYFCKVPLLGTELAASVPKTEIVGKGDYFRFGMRDSLAIEASFL------ 120

Query: 149 MEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWK 208
                                                           KREEELLSIWWK
Sbjct: 121 ------------------------------------------------KREEELLSIWWK 180

Query: 209 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 268
           E AECSEGPKER+G+  RLDKQR+ESASEVAKL+KLYDIEEERVGV VKGGLYEVDLVKR
Sbjct: 181 EYAECSEGPKERSGSSQRLDKQRHESASEVAKLVKLYDIEEERVGVPVKGGLYEVDLVKR 240

Query: 269 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAP 328
           HCFPVYWNAENRRVMRGHWFA KGGLDWLP+REDVAEQLEIAY SR +  +  F     P
Sbjct: 241 HCFPVYWNAENRRVMRGHWFARKGGLDWLPIREDVAEQLEIAYRSR-VWRRRAFQ----P 300

Query: 329 ENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 388
             + A R   +  STPGLHALFTGED+TWEAWLNVDASGFSSVI+LGGNGIKLRRGYS S
Sbjct: 301 SGLFASRV-DLQGSTPGLHALFTGEDNTWEAWLNVDASGFSSVIALGGNGIKLRRGYSPS 360

Query: 389 NAPKPTQDDLRQQREEEMDDY----------FSIH------------------------- 448
           N+PKPTQDDLRQQ+EEEMDDY          F +H                         
Sbjct: 361 NSPKPTQDDLRQQKEEEMDDYCSQVPVRHLVFMVHGIGQRLEKSNLVDDVGNFRHITSSL 420

Query: 449 ------------------------------------------------------------ 508
                                                                       
Sbjct: 421 GERHLTLHQRSTQRVLFIPCQWRKGLKLSGEAAVEKITLDGVKGLRVMLGATAHDVLYYM 480

Query: 509 -----------------------------------------------------------F 568
                                                                      F
Sbjct: 481 SPIYCQDIINSVSNQLNRLYMKFLRRNPGYDGKVSIYGHSLGSVLSYDILCHQENLSSPF 540

Query: 569 VIDIQYDLPD-------------------------------------------------- 628
            +D  Y  P                                                   
Sbjct: 541 PMDWMYAEPTRSEGFSGVDNKSSTRNSSCSTEDKSSTAAYGTADIVHFTKEDERNVHEMH 600

Query: 629 ---SGPHDFYDP------------------------------------------------ 640
                P  F DP                                                
Sbjct: 601 LHLEDPSIFLDPVTSNSAELITTHENTSGGTEYDSSKRLPHTSDGLEEFKNWNCELEVPS 660

BLAST of CmaCh04G030260 vs. ExPASy TrEMBL
Match: A0A6J1JYF1 (phospholipase SGR2 OS=Cucurbita maxima OX=3661 GN=LOC111489011 PE=4 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 1.4e-204
Identity = 461/1002 (46.01%), Postives = 495/1002 (49.40%), Query Frame = 0

Query: 29  MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHC---------- 88
           MDVNSSK+M    ML  AAIGSS V EASPDSLKNTPSNIAKLEDVIEHC          
Sbjct: 1   MDVNSSKNMGDDGMLISAAIGSSVVPEASPDSLKNTPSNIAKLEDVIEHCVGRQKYLAQT 60

Query: 89  --------------------EELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVR 148
                                E+AASVPKTEIVGKG+Y RFGMRDSLAIEASFLQ     
Sbjct: 61  RSPSDGGDVRWYFCKVPLLGNEVAASVPKTEIVGKGDYLRFGMRDSLAIEASFLQ----- 120

Query: 149 MEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWK 208
                                                            REEELLSIWWK
Sbjct: 121 -------------------------------------------------REEELLSIWWK 180

Query: 209 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 268
           E AECSEGPKERAG+ L++DKQR ESASEVAKL+KLYDIEEERVGV VKGGLYEVDLVKR
Sbjct: 181 EYAECSEGPKERAGSSLKVDKQRNESASEVAKLVKLYDIEEERVGVPVKGGLYEVDLVKR 240

Query: 269 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAP 328
           HCFPVYWNAENRRVMRGHWFA KGGLDWLPLREDV EQLEIAY S Q+  +  F     P
Sbjct: 241 HCFPVYWNAENRRVMRGHWFARKGGLDWLPLREDVGEQLEIAYRS-QVWHRRTFQ----P 300

Query: 329 ENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 388
             + A R   +  STPGLHALFTGEDDTWEAWLNVD SGFSSVISLGGNGIKLRRGYS S
Sbjct: 301 SGLFASRV-DLQGSTPGLHALFTGEDDTWEAWLNVDTSGFSSVISLGGNGIKLRRGYSPS 360

Query: 389 NAPKPTQDDLRQQREEEMDDY----------FSIHFV---------ID------------ 448
           N+PKPTQDDLRQQREEEMDDY          F +H +         +D            
Sbjct: 361 NSPKPTQDDLRQQREEEMDDYCSQVPVRHLVFMVHGIGQRLEKSNLVDDVGNFRHITSSL 420

Query: 449 ------------------------------------------------------------ 508
                                                                       
Sbjct: 421 GERHLTLHQRSTQRVLFIPCQWRKGLKLSGEAAVEKITLDGVKGLRVMLGATAHDVLYYM 480

Query: 509 --------------------------------------------IQYDL----------- 568
                                                       + YD+           
Sbjct: 481 SPIYCQDIINSVSNQLNRLYMKFLRRNPGYDGKVSIYGHSLGSVLSYDILCHQKNLSSPF 540

Query: 569 -------------PDSGPHD---------------------------------------- 628
                          SG HD                                        
Sbjct: 541 PMDWLYGEHARSEGFSGVHDQSSAQNSSCNTENNCSTAVYGCADIVQLAKENGERNMHLE 600

Query: 629 ----FYDP---------------------------------------------------- 640
                 DP                                                    
Sbjct: 601 DPSIVVDPVPSHSSDLIIKHENACGEADYDSSKRLPQTSDELEELNKNENCDLEVPSLNR 660

BLAST of CmaCh04G030260 vs. NCBI nr
Match: XP_022990768.1 (LOW QUALITY PROTEIN: phospholipase SGR2-like [Cucurbita maxima])

HSP 1 Score: 994.2 bits (2569), Expect = 5.2e-286
Identity = 515/657 (78.39%), Postives = 519/657 (79.00%), Query Frame = 0

Query: 29  MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCE--------- 88
           MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCE         
Sbjct: 1   MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCEGRQKYLAQT 60

Query: 89  ---------------------ELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVR 148
                                ELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQ     
Sbjct: 61  RSPSDGGDVRWYFCKVPLLGTELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQ----- 120

Query: 149 MEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWK 208
                                                            REEELLSIWWK
Sbjct: 121 -------------------------------------------------REEELLSIWWK 180

Query: 209 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 268
           EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR
Sbjct: 181 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 240

Query: 269 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAP 328
           HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSR    +     G+  
Sbjct: 241 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRVWRRRTFQPSGLFA 300

Query: 329 ENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 388
             +       +  STPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS
Sbjct: 301 SXVD------LQGSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 360

Query: 389 NAPKPTQDDLRQQREEEMDDYFSIHFVIDIQYDLPDSGPHDFYDPKSLFVWSSGKRKEYW 448
           NAPKPTQDDLRQQREEEMDDY               S PHDFYDPKSLFVWSSGKRKEYW
Sbjct: 361 NAPKPTQDDLRQQREEEMDDYC--------------SQPHDFYDPKSLFVWSSGKRKEYW 420

Query: 449 DEENISEEMPACRQMLNIFHPFDPVA----------------YRIEPLVCKEYMLKRPVI 508
           DEENISEEMPACRQMLNIFHPFDPVA                YRIEPLVCKEYMLKRPVI
Sbjct: 421 DEENISEEMPACRQMLNIFHPFDPVAYSIPYGERIMEALLDLYRIEPLVCKEYMLKRPVI 480

Query: 509 IPFHRGGRRLHIGFREFTDNLALRSQAKKDDLHTLGVKVLTVCQSKQADGLEEGDENFQG 568
           IPFHRGGRRLHIGFREFTDNLALRSQAKKDDLHTLGVKVLTVCQSKQADGLEEGDENFQG
Sbjct: 481 IPFHRGGRRLHIGFREFTDNLALRSQAKKDDLHTLGVKVLTVCQSKQADGLEEGDENFQG 540

Query: 569 ELKCYGVAMMERLTGSEGGRIDHMLQDKTCEHPYMQAIKSHTNYWRDHDTAIFILKHLYR 628
           ELKCYGVAMMERLTGSEGGRIDHMLQDKTCEHPYMQAIKSHTNYWRDHDTAIFILKHLYR
Sbjct: 541 ELKCYGVAMMERLTGSEGGRIDHMLQDKTCEHPYMQAIKSHTNYWRDHDTAIFILKHLYR 583

Query: 629 DILEDPDTPPEYTEPDSNDCWYNRRETIEEELSLTFSNENVVRNFSRKAKKMIKNHR 640
           DILEDPDTPPEYTEPDSNDCWYNRRETIEEELSLTFSNENVVRNFSRKAKKMIKNHR
Sbjct: 601 DILEDPDTPPEYTEPDSNDCWYNRRETIEEELSLTFSNENVVRNFSRKAKKMIKNHR 583

BLAST of CmaCh04G030260 vs. NCBI nr
Match: XP_023549990.1 (phospholipase SGR2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.5 bits (2035), Expect = 4.3e-224
Identity = 498/1007 (49.45%), Postives = 511/1007 (50.74%), Query Frame = 0

Query: 29  MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCE--------- 88
           MDVNSSKDMV GDMLSLAAIGSSEVSEA+PDSLKNTPSNIAKLEDVIEHCE         
Sbjct: 1   MDVNSSKDMVGGDMLSLAAIGSSEVSEATPDSLKNTPSNIAKLEDVIEHCEGRQKYLAQT 60

Query: 89  ---------------------ELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVR 148
                                ELAASVPKTEIVGKG+YFRFGMRDSLAIEASFLQ     
Sbjct: 61  RSPSDGGDVRWYFCKVPLLGNELAASVPKTEIVGKGDYFRFGMRDSLAIEASFLQ----- 120

Query: 149 MEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWK 208
                                                            REEELLSIWWK
Sbjct: 121 -------------------------------------------------REEELLSIWWK 180

Query: 209 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 268
           EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGV VKGGLYEVDLVKR
Sbjct: 181 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVPVKGGLYEVDLVKR 240

Query: 269 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAP 328
           HCFPVYWNAENRRVMRGHWFA KGGLDWLPLREDVAEQLEIAY SR +  +  F     P
Sbjct: 241 HCFPVYWNAENRRVMRGHWFARKGGLDWLPLREDVAEQLEIAYRSR-VWCRRTFQ----P 300

Query: 329 ENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 388
             + A R   +  ST GLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS
Sbjct: 301 SGLFASRV-DLQGSTLGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 360

Query: 389 NAPKPTQDDLRQQREEEMDDY----------FSIH------------------------- 448
           NAPKPTQDDLRQQREEEMDDY          F +H                         
Sbjct: 361 NAPKPTQDDLRQQREEEMDDYCSQVPVRHLVFMVHGIGQRLEKSNLVDDVGNFRHITSSL 420

Query: 449 ------------------------------------------------------------ 508
                                                                       
Sbjct: 421 GERHLTFHQRSTQRVLFIPCQWRKGLKLSGEAAVEKITLDGVKGLRVMLGATAHDVLYYM 480

Query: 509 -----------------------------------------------------------F 568
                                                                      F
Sbjct: 481 SPIYCQDIINSVSNQLNRLYMKFLRRNPGYDGKVSIYGHSLGSVLSYDILCHQDNLSSPF 540

Query: 569 VIDIQYD----------------------------------------------------- 628
            +D  Y                                                      
Sbjct: 541 PMDWMYGEHTRSEGFSGVDNESFTQNSSCSTGDKYSTAVYGCADIAHFAEEEDERNVNQM 600

Query: 629 -------------------------------------------------LPDSGPHDFYD 640
                                                            L  +G  D  D
Sbjct: 601 HLHMEEPSIVVDPTTSHSSDFISKQENTYGEADYESCKRIPLTSDGFQRLNKNGNCDLED 660

BLAST of CmaCh04G030260 vs. NCBI nr
Match: XP_023549980.1 (phospholipase SGR2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 778.1 bits (2008), Expect = 5.9e-221
Identity = 498/1023 (48.68%), Postives = 511/1023 (49.95%), Query Frame = 0

Query: 29  MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCE--------- 88
           MDVNSSKDMV GDMLSLAAIGSSEVSEA+PDSLKNTPSNIAKLEDVIEHCE         
Sbjct: 1   MDVNSSKDMVGGDMLSLAAIGSSEVSEATPDSLKNTPSNIAKLEDVIEHCEGRQKYLAQT 60

Query: 89  ---------------------ELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVR 148
                                ELAASVPKTEIVGKG+YFRFGMRDSLAIEASFLQ     
Sbjct: 61  RSPSDGGDVRWYFCKVPLLGNELAASVPKTEIVGKGDYFRFGMRDSLAIEASFLQ----- 120

Query: 149 MEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWK 208
                                                            REEELLSIWWK
Sbjct: 121 -------------------------------------------------REEELLSIWWK 180

Query: 209 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 268
           EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGV VKGGLYEVDLVKR
Sbjct: 181 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVPVKGGLYEVDLVKR 240

Query: 269 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAP 328
           HCFPVYWNAENRRVMRGHWFA KGGLDWLPLREDVAEQLEIAY SR +  +  F     P
Sbjct: 241 HCFPVYWNAENRRVMRGHWFARKGGLDWLPLREDVAEQLEIAYRSR-VWCRRTFQ----P 300

Query: 329 ENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 388
             + A R   +  ST GLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS
Sbjct: 301 SGLFASRV-DLQGSTLGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 360

Query: 389 NAPKPTQDDLRQQREEEMDDY----------FSIH------------------------- 448
           NAPKPTQDDLRQQREEEMDDY          F +H                         
Sbjct: 361 NAPKPTQDDLRQQREEEMDDYCSQVPVRHLVFMVHGIGQRLEKSNLVDDVGNFRHITSSL 420

Query: 449 ------------------------------------------------------------ 508
                                                                       
Sbjct: 421 GERHLTFHQRSTQRVLFIPCQWRKGLKLSGEAAVEKITLDGVKGLRVMLGATAHDVLYYM 480

Query: 509 -----------------------------------------------------------F 568
                                                                      F
Sbjct: 481 SPIYCQDIINSVSNQLNRLYMKFLRRNPGYDGKVSIYGHSLGSVLSYDILCHQDNLSSPF 540

Query: 569 VIDIQYD----------------------------------------------------- 628
            +D  Y                                                      
Sbjct: 541 PMDWMYGEHTRSEGFSGVDNESFTQNSSCSTGDKYSTAVYGCADIAHFAEEEDERNVNQM 600

Query: 629 -------------------------------------------------LPDSGPHDFYD 640
                                                            L  +G  D  D
Sbjct: 601 HLHMEEPSIVVDPTTSHSSDFISKQENTYGEADYESCKRIPLTSDGFQRLNKNGNCDLED 660

BLAST of CmaCh04G030260 vs. NCBI nr
Match: KAG7015888.1 (Phospholipase SGR2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 734.9 bits (1896), Expect = 5.7e-208
Identity = 470/1014 (46.35%), Postives = 504/1014 (49.70%), Query Frame = 0

Query: 17  GSEARIIRSGAKMDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIE 76
           GSEA+II S A MDVNSSK+M   DML  AAIGSS V EASPDSLKNTPSNIAKLEDVIE
Sbjct: 2   GSEAKIIVSEAGMDVNSSKNMGDDDMLISAAIGSSVVPEASPDSLKNTPSNIAKLEDVIE 61

Query: 77  HC------------------------------EELAASVPKTEIVGKGEYFRFGMRDSLA 136
           HC                               E+AASVPKTEIVGKG+Y RFGMRDSLA
Sbjct: 62  HCVGRQKYLAQTRSPSDGGDVRWYFCKVPLLGNEVAASVPKTEIVGKGDYLRFGMRDSLA 121

Query: 137 IEASFLQTSPVRMEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLN 196
           IEASFLQ                                                     
Sbjct: 122 IEASFLQ----------------------------------------------------- 181

Query: 197 KREEELLSIWWKEIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHV 256
            REEELLSIWWKE AECSEGPKERAG+ L++DKQR ESASEVAKL KLYDIEEERVGV V
Sbjct: 182 -REEELLSIWWKEYAECSEGPKERAGSSLKIDKQRNESASEVAKLAKLYDIEEERVGVPV 241

Query: 257 KGGLYEVDLVKRHCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQI 316
           KGGLYEVDLVKRHCFPVYWNAENRRVMRGHWFA KGGLDWLPLREDV EQLEIAY S Q+
Sbjct: 242 KGGLYEVDLVKRHCFPVYWNAENRRVMRGHWFARKGGLDWLPLREDVGEQLEIAYRS-QV 301

Query: 317 MDKGDFDLGVAPENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGG 376
             +  F     P  + A R   +  STPGLHALFTGEDDTWEAWLNVD SGFSSVISLGG
Sbjct: 302 WHRRTFQ----PSGLFASRV-DLQGSTPGLHALFTGEDDTWEAWLNVDTSGFSSVISLGG 361

Query: 377 NGIKLRRGYSLSNAPKPTQDDLRQQREEEMDDY----------FSIHFV---------ID 436
           NGIKLRRGYS SN+PKPTQDDLRQQREEEMDDY          F +H +         +D
Sbjct: 362 NGIKLRRGYSPSNSPKPTQDDLRQQREEEMDDYCSQVPVRHLVFMVHGIGQRLEKSNLVD 421

Query: 437 ------------------------------------------------------------ 496
                                                                       
Sbjct: 422 DVGNFRHITSSLGERHLTLHQRSTQRVLFIPCQWRKGLKLSGEAAVEKITLDGVKGLRVM 481

Query: 497 --------------------------------------------------------IQYD 556
                                                                   + YD
Sbjct: 482 LGATAHDVLYYMSPIYCQDIINSVSNQLNRLYMKFLRRNPGYDGKVSIYGHSLGSVLSYD 541

Query: 557 L------------------------PDSGPHD---------------------------- 616
           +                          SG HD                            
Sbjct: 542 ILCHQKNLSSPFPMDWMYGEHARSEGFSGVHDQSSAQNSSCNTENNCSTAVYGCADIVQL 601

Query: 617 ----------FYDP---------------------------------------------- 640
                       DP                                              
Sbjct: 602 AKEDGERNMHLEDPSIVADPVPLHSSDLIIKHENACGEADYDSSKRLPQTSDELEELNKN 661

BLAST of CmaCh04G030260 vs. NCBI nr
Match: XP_022152436.1 (phospholipase SGR2 isoform X3 [Momordica charantia])

HSP 1 Score: 728.4 bits (1879), Expect = 5.3e-206
Identity = 458/983 (46.59%), Postives = 496/983 (50.46%), Query Frame = 0

Query: 29  MDVNSSKDMVRGDMLSLAAIGSSEVSEASPDSLKNTPSNIAKLEDVIEHCE--------- 88
           MD NS++D+  GDML+ +AIGSSEV EASPDSLKNTPSNIAKLEDVIEHCE         
Sbjct: 1   MDANSTEDIGGGDMLTSSAIGSSEVPEASPDSLKNTPSNIAKLEDVIEHCEGRQKYLAHT 60

Query: 89  ---------------------ELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVR 148
                                ELAASVPKTEIVGKG+YFRFGMRDSLAIEASFL      
Sbjct: 61  RSPSDGGDVRWYFCKVPLLGTELAASVPKTEIVGKGDYFRFGMRDSLAIEASFL------ 120

Query: 149 MEGRKFQKFLAKGRKVRYDSWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWK 208
                                                           KREEELLSIWWK
Sbjct: 121 ------------------------------------------------KREEELLSIWWK 180

Query: 209 EIAECSEGPKERAGTGLRLDKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKR 268
           E AECSEGPKER+G+  RLDKQR+ESASEVAKL+KLYDIEEERVGV VKGGLYEVDLVKR
Sbjct: 181 EYAECSEGPKERSGSSQRLDKQRHESASEVAKLVKLYDIEEERVGVPVKGGLYEVDLVKR 240

Query: 269 HCFPVYWNAENRRVMRGHWFACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAP 328
           HCFPVYWNAENRRVMRGHWFA KGGLDWLP+REDVAEQLEIAY SR +  +  F     P
Sbjct: 241 HCFPVYWNAENRRVMRGHWFARKGGLDWLPIREDVAEQLEIAYRSR-VWRRRAFQ----P 300

Query: 329 ENISAIRTFCILRSTPGLHALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLS 388
             + A R   +  STPGLHALFTGED+TWEAWLNVDASGFSSVI+LGGNGIKLRRGYS S
Sbjct: 301 SGLFASRV-DLQGSTPGLHALFTGEDNTWEAWLNVDASGFSSVIALGGNGIKLRRGYSPS 360

Query: 389 NAPKPTQDDLRQQREEEMDDY----------FSIH------------------------- 448
           N+PKPTQDDLRQQ+EEEMDDY          F +H                         
Sbjct: 361 NSPKPTQDDLRQQKEEEMDDYCSQVPVRHLVFMVHGIGQRLEKSNLVDDVGNFRHITSSL 420

Query: 449 ------------------------------------------------------------ 508
                                                                       
Sbjct: 421 GERHLTLHQRSTQRVLFIPCQWRKGLKLSGEAAVEKITLDGVKGLRVMLGATAHDVLYYM 480

Query: 509 ---------------------------------------FVIDIQYDLPD---------- 568
                                                  F +D  Y  P           
Sbjct: 481 SPIYCQDIINSVSNQLNRLYMKFLRRNPGYDGKENLSSPFPMDWMYAEPTRSEGFSGVDN 540

Query: 569 -------------------------------------------SGPHDFYDP-------- 628
                                                        P  F DP        
Sbjct: 541 KSSTRNSSCSTEDKSSTAAYGTADIVHFTKEDERNVHEMHLHLEDPSIFLDPVTSNSAEL 600

Query: 629 -----------------------------------------------------------K 640
                                                                      K
Sbjct: 601 ITTHENTSGGTEYDSSKRLPHTSDGLEEFKNWNCELEVPSVNKVAELQFEDSIDKDEAIK 660

BLAST of CmaCh04G030260 vs. TAIR 10
Match: AT1G31480.1 (shoot gravitropism 2 (SGR2) )

HSP 1 Score: 478.8 bits (1231), Expect = 6.9e-135
Identity = 332/988 (33.60%), Postives = 420/988 (42.51%), Query Frame = 0

Query: 48  IGSSEVSEASPDSLKNTPSNIAKLEDVIEHC----------------------------- 107
           +G+ EV+E SPD LKNTPSNIA+LEDVIE C                             
Sbjct: 8   LGTREVNETSPDLLKNTPSNIARLEDVIEQCHGRQKYLAQTRSPSDGSDVRWYFCKVPLA 67

Query: 108 -EELAASVPKTEIVGKGEYFRFGMRDSLAIEASFLQTSPVRMEGRKFQKFLAKGRKVRYD 167
             ELAASVP+T++VGK EYFRFGMRDSLAIEASFLQ                        
Sbjct: 68  ENELAASVPRTDVVGKSEYFRFGMRDSLAIEASFLQ------------------------ 127

Query: 168 SWHKAYHYGDNAIKWKKACNDSLHFLHLNKREEELLSIWWKEIAECSEGPKERAGTGLRL 227
                                         RE+ELLS+WWKE AECSEGPK +  +    
Sbjct: 128 ------------------------------REDELLSLWWKEYAECSEGPKLQVNS---- 187

Query: 228 DKQRYESASEVAKLIKLYDIEEERVGVHVKGGLYEVDLVKRHCFPVYWNAENRRVMRGHW 287
            K+  E+ SE +    LY++EEERVGV VKGGLYEVDLV+RHCFPVYWN +NRRV+RGHW
Sbjct: 188 KKKSIETPSEASVSSSLYEVEEERVGVPVKGGLYEVDLVRRHCFPVYWNGDNRRVLRGHW 247

Query: 288 FACKGGLDWLPLREDVAEQLEIAYCSRQIMDKGDFDLGVAPENISAIRTFCILRSTPGLH 347
           FA KGGLDWLP+ E V+EQLE+AY   ++  +  F     P  + A R   +  S+ GLH
Sbjct: 248 FARKGGLDWLPIPETVSEQLEVAY-RNKVWRRRSFQ----PSGLFAAR-IDLQGSSLGLH 307

Query: 348 ALFTGEDDTWEAWLNVDASGFSSVISLGGNGIKLRRGYSLSNAPKPTQDDLRQQREEEMD 407
           ALFTGEDDTWEAWLNVD SGFS ++   GNGIKLRRGY+ S +PKPTQ++LRQQ+EEEMD
Sbjct: 308 ALFTGEDDTWEAWLNVDPSGFSGIVGYTGNGIKLRRGYAGSYSPKPTQEELRQQKEEEMD 367

Query: 408 DY----------FSIH-------------------------------------------- 467
           DY          F +H                                            
Sbjct: 368 DYCSQVPVRHLVFMVHGIGQKGEKSNLVDDVGNFRQITAALAERHLTSHQLSTQRVLFIP 427

Query: 468 -----------------------------------------------FVID--------- 527
                                                           +ID         
Sbjct: 428 CQWRKGLKLSGEAAVDKCTLDGVRRFREMLSATVHDVLYYMSPIYCQAIIDSVSKQLNRL 487

Query: 528 -------------------------IQYDL---------------------PD------- 587
                                    + YD+                     PD       
Sbjct: 488 YLKFLKRNPDYVGKISIYGHSLGSVLSYDILCHQHNLSSPFPMDSVYKKFFPDEESPPTP 547

Query: 588 --------SGPHDFYDPK------------------------------------------ 637
                   S P   ++P+                                          
Sbjct: 548 AKADKPCSSHPSSNFEPEKSDQLNNPEKITGQDNNTMAKEPTVLEHHDVIQEDPSLISDS 607