CmaCh04G030200 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G030200
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCma_Chr04: 19715716 .. 19716726 (-)
RNA-Seq ExpressionCmaCh04G030200
SyntenyCmaCh04G030200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCCACTGGCGACACTCAATCTCCTTCCTCATCTCCCAACCTTCCATCCAAGCGTAAGCTTGCCGATCTATTCACTTCCGTCCTTTTCCGACATGTTTTCCGATCAGATTTTACAGTGAACCGCGGTCTCCTCCGCCTACTCGACCGTAAAGTACCTCCCTCCACCACTCGCGGCGTCGCCTCCTTCGACACCACACTCGATTCTTCATCCTCCGACCTCTGGCTTCGTGTTTATAATCCTCCCACATGTTCCGAGTCCGATCGTATGCCTGTTATCATATACTTCCACGGTGGAGGCTTTGTATTCGGCTCCGCCGATTCCCAGCAGATCGACTCGTTCTCTCGCGATTTTGCTCGCGAAATCGGAGCCATTGTCGTCTCCGTCAACTACCGCCTCGCGCCGGAGCACCGATTTCCGAGCCCATACGACGACGGATTTCGCGTTCTGAAGGCAATCGACGAAGGATCGATCGCCAAAAGTCTTCCAGAAAATGCTGATATAAGCCGCTGTTTCATCGCCGGCGAAAGCGCCGGAGGTAACATCGTGCATCACGTGACGGTGAGAGCGGCGAAATATGAGTTCAAGAGGCTTAGGATCGCAGGTATGATTGCCATACAGCCATTTTTGGGAGGAGAAGAGCGGACACAATCAGAGATTCGATTTGGGAGAGGCGGTGGACTGACTTTGGAAACAGCGGATTGGTATTGGAAGGCGTTTCTACCGATCGGTTCGAGCCGGGATCACCCGGCTGCTAACGTGGTTCGGTCCGGGTCCGATGTTTCGGGGCTGAAATTACCACCGGCGCTTGTTGTGAATGGGGGCTTGGATTTGTTAAGCGATCGGATCAAAGAGTACTGTGAGTGGCTGGAGAAATCGGGTAAAGAGGTTCGGGTCGTGGAGTATCCGAATGGGATTCATGGGTTTAGTTGGTTTTCAGATCTTCCGGAATATTCCATGCTGATGCAAGACGCCAGAGAATTCACCAGAAGGGTCTTGTTAAATTAA

mRNA sequence

ATGTCTTCCACTGGCGACACTCAATCTCCTTCCTCATCTCCCAACCTTCCATCCAAGCGTAAGCTTGCCGATCTATTCACTTCCGTCCTTTTCCGACATGTTTTCCGATCAGATTTTACAGTGAACCGCGGTCTCCTCCGCCTACTCGACCGTAAAGTACCTCCCTCCACCACTCGCGGCGTCGCCTCCTTCGACACCACACTCGATTCTTCATCCTCCGACCTCTGGCTTCGTGTTTATAATCCTCCCACATGTTCCGAGTCCGATCGTATGCCTGTTATCATATACTTCCACGGTGGAGGCTTTGTATTCGGCTCCGCCGATTCCCAGCAGATCGACTCGTTCTCTCGCGATTTTGCTCGCGAAATCGGAGCCATTGTCGTCTCCGTCAACTACCGCCTCGCGCCGGAGCACCGATTTCCGAGCCCATACGACGACGGATTTCGCGTTCTGAAGGCAATCGACGAAGGATCGATCGCCAAAAGTCTTCCAGAAAATGCTGATATAAGCCGCTGTTTCATCGCCGGCGAAAGCGCCGGAGGTAACATCGTGCATCACGTGACGGTGAGAGCGGCGAAATATGAGTTCAAGAGGCTTAGGATCGCAGGTATGATTGCCATACAGCCATTTTTGGGAGGAGAAGAGCGGACACAATCAGAGATTCGATTTGGGAGAGGCGGTGGACTGACTTTGGAAACAGCGGATTGGTATTGGAAGGCGTTTCTACCGATCGGTTCGAGCCGGGATCACCCGGCTGCTAACGTGGTTCGGTCCGGGTCCGATGTTTCGGGGCTGAAATTACCACCGGCGCTTGTTGTGAATGGGGGCTTGGATTTGTTAAGCGATCGGATCAAAGAGTACTGTGAGTGGCTGGAGAAATCGGGTAAAGAGGTTCGGGTCGTGGAGTATCCGAATGGGATTCATGGGTTTAGTTGGTTTTCAGATCTTCCGGAATATTCCATGCTGATGCAAGACGCCAGAGAATTCACCAGAAGGGTCTTGTTAAATTAA

Coding sequence (CDS)

ATGTCTTCCACTGGCGACACTCAATCTCCTTCCTCATCTCCCAACCTTCCATCCAAGCGTAAGCTTGCCGATCTATTCACTTCCGTCCTTTTCCGACATGTTTTCCGATCAGATTTTACAGTGAACCGCGGTCTCCTCCGCCTACTCGACCGTAAAGTACCTCCCTCCACCACTCGCGGCGTCGCCTCCTTCGACACCACACTCGATTCTTCATCCTCCGACCTCTGGCTTCGTGTTTATAATCCTCCCACATGTTCCGAGTCCGATCGTATGCCTGTTATCATATACTTCCACGGTGGAGGCTTTGTATTCGGCTCCGCCGATTCCCAGCAGATCGACTCGTTCTCTCGCGATTTTGCTCGCGAAATCGGAGCCATTGTCGTCTCCGTCAACTACCGCCTCGCGCCGGAGCACCGATTTCCGAGCCCATACGACGACGGATTTCGCGTTCTGAAGGCAATCGACGAAGGATCGATCGCCAAAAGTCTTCCAGAAAATGCTGATATAAGCCGCTGTTTCATCGCCGGCGAAAGCGCCGGAGGTAACATCGTGCATCACGTGACGGTGAGAGCGGCGAAATATGAGTTCAAGAGGCTTAGGATCGCAGGTATGATTGCCATACAGCCATTTTTGGGAGGAGAAGAGCGGACACAATCAGAGATTCGATTTGGGAGAGGCGGTGGACTGACTTTGGAAACAGCGGATTGGTATTGGAAGGCGTTTCTACCGATCGGTTCGAGCCGGGATCACCCGGCTGCTAACGTGGTTCGGTCCGGGTCCGATGTTTCGGGGCTGAAATTACCACCGGCGCTTGTTGTGAATGGGGGCTTGGATTTGTTAAGCGATCGGATCAAAGAGTACTGTGAGTGGCTGGAGAAATCGGGTAAAGAGGTTCGGGTCGTGGAGTATCCGAATGGGATTCATGGGTTTAGTTGGTTTTCAGATCTTCCGGAATATTCCATGCTGATGCAAGACGCCAGAGAATTCACCAGAAGGGTCTTGTTAAATTAA

Protein sequence

MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTRGVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESAGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Homology
BLAST of CmaCh04G030200 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.3e-70
Identity = 144/328 (43.90%), Postives = 200/328 (60.98%), Query Frame = 0

Query: 10  PSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPS--TTRGVASFDTT 69
           P+    LP K ++A    S +  +  R D T+NR  LRL D + PP+      V++ D  
Sbjct: 7   PNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFV 66

Query: 70  LDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDFAREIGAIV 129
           +D  S DLW R+Y P      D++PV+++FHGGGF F S ++   D+  R FAR++ A V
Sbjct: 67  VD-QSRDLWFRLYTPHV--SGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYV 126

Query: 130 VSVNYRLAPEHRFPSPYDDGFRVLKAIDE--GSIAKSLPENADISRCFIAGESAGGNIVH 189
           +SVNYRLAPEHR+P+ YDDGF  LK I+E  GSI   LP NAD+SRCF AG+SAGGNI H
Sbjct: 127 ISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSI---LPANADLSRCFFAGDSAGGNIAH 186

Query: 190 HVTVRAA---KYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKAFL 249
           +V +R     +  F  +++ G+I+IQPF GGEERT++E +      ++ +  DW WKA  
Sbjct: 187 NVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA-- 246

Query: 250 PIGSSRDHPAANVVRSGS-DVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV 309
            +G +RDH A NV    + D+SGL  P  +VV  G D L D  + Y EWL+  GK+  ++
Sbjct: 247 -MGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLI 306

Query: 310 EYPNGIHGFSWFSDLPEYSMLMQDAREF 330
           EYPN  H F  F +LPE   L+   ++F
Sbjct: 307 EYPNMFHAFYIFPELPEAGQLIMRIKDF 325

BLAST of CmaCh04G030200 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.3e-54
Identity = 131/341 (38.42%), Postives = 182/341 (53.37%), Query Frame = 0

Query: 11  SSSPNLPSKRKLADLFTSVL-------FRHVFRSDFTVNRGLLRLLDRKVPPST--TRGV 70
           S   NL   + +  L T VL       +  + R D T NR L   LDRKVP +     GV
Sbjct: 4   SEEVNLIESKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGV 63

Query: 71  ASFDTTLDSSSSDLWLRVYNPPTCSES------------DRMPVIIYFHGGGFVFGSADS 130
            SFD  +D   ++L  RVY P     S            + +PVI++FHGG F   SA+S
Sbjct: 64  FSFDVIID-RQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANS 123

Query: 131 QQIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADI 190
              D+  R      GA+VVSVNYR APE+R+P  YDDG+ VLK ++  S  +S  ++   
Sbjct: 124 AIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNSSSWLRSKKDSK-- 183

Query: 191 SRCFIAGESAGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGL 250
            R F+AG+S+GGNIVH+V VRA +    R+ + G I + P  GG ERT+SE R      +
Sbjct: 184 VRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFV 243

Query: 251 TLETADWYWKAFLPIGSSRDHPAANVVRSGS-DVSGLKLPPALVVNGGLDLLSDRIKEYC 310
           T+   DWYW+AFLP G  R+HPA +     S  + GL  P +LVV  GLDL+ D   +Y 
Sbjct: 244 TVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYA 303

Query: 311 EWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 330
           E L+K+G+EV+++       GF    +   +  +M +   F
Sbjct: 304 EGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAF 338

BLAST of CmaCh04G030200 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.2e-51
Identity = 120/318 (37.74%), Postives = 167/318 (52.52%), Query Frame = 0

Query: 36  RSDFTVNRGLLRLLDRKVPPST--TRGVASFDTTLDSSSSDLWLRVYNPPTCSESDR--- 95
           R+D T  R L   LDR+VP +     GV+SFD  +D S   L +R+Y      +++    
Sbjct: 36  RADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVG-LEVRIYRAAAEGDAEEGAA 95

Query: 96  ------------------MPVIIYFHGGGFVFGSADSQQIDSFSRDFAREIGAIVVSVNY 155
                              PVII+FHGG FV  SA S   DS  R F +    +VVSVNY
Sbjct: 96  AVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGVVVSVNY 155

Query: 156 RLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESAGGNIVHHVTVRAA 215
           R APEHR+P  YDDG+  LK +      +S  +    +R F++G+S+GGNI HHV VRAA
Sbjct: 156 RRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQ--ARVFLSGDSSGGNIAHHVAVRAA 215

Query: 216 KYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKAFLPIGSSRDHPA 275
               + +++ G I +    GG ERT+SE R      +TL+  DWYWKA+LP  + RDHPA
Sbjct: 216 D---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDADRDHPA 275

Query: 276 ANVV-RSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVVEYPNGIHGFS 330
            N    +G  + GL    +L++  GLDL  DR   Y + L + G  V+VV+  N   GF 
Sbjct: 276 CNPFGPNGRRLGGLPFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCENATVGFY 335

BLAST of CmaCh04G030200 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.6e-50
Identity = 120/344 (34.88%), Postives = 182/344 (52.91%), Query Frame = 0

Query: 15  NLPSKRKLADLFTSVL-------FRHVFRSDFTVNRGLLRLLDRKVPPST--TRGVASFD 74
           NL   +++  L T VL       ++ + R D + NR L   LDRKVP ++    GV SFD
Sbjct: 8   NLNECKRIVPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD 67

Query: 75  TTLDSSSSDLWLRVYNPPT---------------CSESDRMPVIIYFHGGGFVFGSADSQ 134
                S+++L  R+Y P +                S ++ +PV+I+FHGG F   SA+S 
Sbjct: 68  HV--DSTTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSA 127

Query: 135 QIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADIS 194
             D+F R      G +VVSV+YR +PEHR+P  YDDG+  L  +      +S  ++    
Sbjct: 128 IYDTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSN--V 187

Query: 195 RCFIAGESAGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLT 254
             ++AG+S+GGNI H+V VRA     + +++ G I + P  GG+ERTQSE        +T
Sbjct: 188 YVYLAGDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVT 247

Query: 255 LETADWYWKAFLPIGSSRDHPAANVV-RSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCE 314
           ++  DWYW+A+LP G  RDHPA N     G  + G+  P +LVV  GLDL+ D    Y +
Sbjct: 248 IQDRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVD 307

Query: 315 WLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 334
            L+K+G EV ++       GF +  +   +  LM++  +F   +
Sbjct: 308 GLKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

BLAST of CmaCh04G030200 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-47
Identity = 123/343 (35.86%), Postives = 170/343 (49.56%), Query Frame = 0

Query: 11  SSSPNLPSKRKLADLFTSVL-------FRHVFRSDFTVNRGLLRLLDRKVPPST--TRGV 70
           S   NL   R +  L T VL       +  + R D T NR L   LDRKV  +     GV
Sbjct: 4   SDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGV 63

Query: 71  ASFDTTLDSSSSDLWLRVYNPPTCS--------------ESDRMPVIIYFHGGGFVFGSA 130
            SFD  +D    +L  RVY P                  + D +PVI++FHGG F   SA
Sbjct: 64  FSFDVLID-RRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSA 123

Query: 131 DSQQIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENA 190
           +S   D+  R        +VVSVNYR APE+ +P  YDDG+  L  ++  S  KS  ++ 
Sbjct: 124 NSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSK 183

Query: 191 DISRCFIAGESAGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGG 250
                F+AG+S+GGNI H+V +RA +     + + G I + P  GG ERT+SE       
Sbjct: 184 --VHIFLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKY 243

Query: 251 GLTLETADWYWKAFLPIGSSRDHPAANVVR-SGSDVSGLKLPPALVVNGGLDLLSDRIKE 310
            +T+   DWYWKAFLP G  R+HPA N     G  + G+  P +LVV  GLDL+ D    
Sbjct: 244 FVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLA 303

Query: 311 YCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 330
           Y E L+K+G+EV+++       GF    +   +  +M +   F
Sbjct: 304 YAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAF 340

BLAST of CmaCh04G030200 vs. ExPASy TrEMBL
Match: A0A6J1JQL4 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111487452 PE=4 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.3e-193
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTRG 60
           MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTRG
Sbjct: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTRG 60

Query: 61  VASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDFA 120
           VASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDFA
Sbjct: 61  VASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDFA 120

Query: 121 REIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESAG 180
           REIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESAG
Sbjct: 121 REIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESAG 180

Query: 181 GNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKA 240
           GNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKA
Sbjct: 181 GNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKA 240

Query: 241 FLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRV 300
           FLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRV
Sbjct: 241 FLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRV 300

Query: 301 VEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 337
           VEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 VEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 336

BLAST of CmaCh04G030200 vs. ExPASy TrEMBL
Match: A0A6J1HPS2 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111464932 PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.3e-180
Identity = 317/337 (94.07%), Postives = 325/337 (96.44%), Query Frame = 0

Query: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPP-STTR 60
           M STGDTQSPSSSPNLPSKR+LADL +SVLFRH FRSDFTVNRG LRLLDRKVPP STTR
Sbjct: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNP TCSESDRMPVIIYFHGGGFVFGSADSQ+IDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESA 180
           AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSI+KSLPENADIS CFIAGESA
Sbjct: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180

Query: 181 GGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWK 240
           GGNIVHHVTVRAA+YEFKRL+IAGMIAIQPFLGGEERT+SEIRFG+GGGLTLE  DWYWK
Sbjct: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240

Query: 241 AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           AFLPIGSSRDHP ANVVR  SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR
Sbjct: 241 AFLPIGSSRDHPGANVVR--SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 337
           VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 335

BLAST of CmaCh04G030200 vs. ExPASy TrEMBL
Match: A0A5A7T4I6 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00840 PE=4 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 2.1e-138
Identity = 248/328 (75.61%), Postives = 279/328 (85.06%), Query Frame = 0

Query: 1   MSSTGDTQSPSSS-PNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTR 60
           M+S GD QSP+SS P LP  RKLADLFTS LFR  FRSDFTVNR LL LLDRKVPP TTR
Sbjct: 1   MASAGDAQSPNSSPPTLPWNRKLADLFTSFLFRRAFRSDFTVNRRLLGLLDRKVPPFTTR 60

Query: 61  GVASFDTTLDSSSSD-LWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRD 120
           GVA+ D  +DSSSSD LW+RVYNP T   SD +PVI++FHGGGFV+GSADS  ID+F RD
Sbjct: 61  GVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVYGSADSPPIDTFCRD 120

Query: 121 FAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGES 180
           FAREIGAIV+SVNYRLAPEHRFPS +DDGF VLKA++EG I+++LPENAD+ RCFIAGES
Sbjct: 121 FAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVISETLPENADLRRCFIAGES 180

Query: 181 AGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYW 240
           AGGNI HHVTVRAA+ E KRL+I GMI IQPFLGGEER  SEIRFGRG G+T+E ADW+W
Sbjct: 181 AGGNIAHHVTVRAAEGELKRLKIVGMILIQPFLGGEERRDSEIRFGRGYGMTVEMADWFW 240

Query: 241 KAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEV 300
           KA+LP+GS+RDHPAANVVR  S +SG+KLPPA+VV GGLDLL DR KEY EWL+K G+EV
Sbjct: 241 KAWLPVGSNRDHPAANVVR--SSISGVKLPPAVVVIGGLDLLRDRQKEYVEWLKKWGEEV 300

Query: 301 RVVEYPNGIHGFSWFSDLPEYSMLMQDA 327
           RVVEYPNG HGF W  DLPEYSMLMQDA
Sbjct: 301 RVVEYPNGTHGFIWKPDLPEYSMLMQDA 326

BLAST of CmaCh04G030200 vs. ExPASy TrEMBL
Match: A0A1S3B2W2 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103485400 PE=4 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 2.1e-138
Identity = 248/328 (75.61%), Postives = 279/328 (85.06%), Query Frame = 0

Query: 1   MSSTGDTQSPSSS-PNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTR 60
           M+S GD QSP+SS P LP  RKLADLFTS LFR  FRSDFTVNR LL LLDRKVPP TTR
Sbjct: 1   MASAGDAQSPNSSPPTLPWNRKLADLFTSFLFRRAFRSDFTVNRRLLGLLDRKVPPFTTR 60

Query: 61  GVASFDTTLDSSSSD-LWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRD 120
           GVA+ D  +DSSSSD LW+RVYNP T   SD +PVI++FHGGGFV+GSADS  ID+F RD
Sbjct: 61  GVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVYGSADSPPIDTFCRD 120

Query: 121 FAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGES 180
           FAREIGAIV+SVNYRLAPEHRFPS +DDGF VLKA++EG I+++LPENAD+ RCFIAGES
Sbjct: 121 FAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVISETLPENADLRRCFIAGES 180

Query: 181 AGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYW 240
           AGGNI HHVTVRAA+ E KRL+I GMI IQPFLGGEER  SEIRFGRG G+T+E ADW+W
Sbjct: 181 AGGNIAHHVTVRAAEGELKRLKIVGMILIQPFLGGEERRDSEIRFGRGYGMTVEMADWFW 240

Query: 241 KAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEV 300
           KA+LP+GS+RDHPAANVVR  S +SG+KLPPA+VV GGLDLL DR KEY EWL+K G+EV
Sbjct: 241 KAWLPVGSNRDHPAANVVR--SSISGVKLPPAVVVIGGLDLLRDRQKEYVEWLKKWGEEV 300

Query: 301 RVVEYPNGIHGFSWFSDLPEYSMLMQDA 327
           RVVEYPNG HGF W  DLPEYSMLMQDA
Sbjct: 301 RVVEYPNGTHGFIWKPDLPEYSMLMQDA 326

BLAST of CmaCh04G030200 vs. ExPASy TrEMBL
Match: A0A0A0LMQ6 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G401430 PE=4 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 4.6e-138
Identity = 243/334 (72.75%), Postives = 283/334 (84.73%), Query Frame = 0

Query: 1   MSSTGDTQSPSSS-PNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTR 60
           M+STGD QSP+SS P LP KRKLADLFTS LFR  FRSDFTVNR  LRL DRK+PP T+R
Sbjct: 1   MASTGDAQSPNSSLPTLPWKRKLADLFTSFLFRQAFRSDFTVNRRFLRLFDRKLPPFTSR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF 120
           GVA+ D T+DSS+SDLW+RVYNP T S SD +PVIIYFHGGGFV+GSAD+   D+F RDF
Sbjct: 61  GVAASDATIDSSTSDLWIRVYNPLTFSNSDPLPVIIYFHGGGFVYGSADAPPTDTFCRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESA 180
           AREIGAIV+SVNYRLAPE RFPS +DDGF VLKA+D+G+I++++PENAD+ RCFIAGESA
Sbjct: 121 AREIGAIVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAISETVPENADLRRCFIAGESA 180

Query: 181 GGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWK 240
           GGNI HHVTVRAA+ EFKR++I GMI IQPF GGEER  SEIRFGRG GLTLE  DW+WK
Sbjct: 181 GGNIAHHVTVRAAESEFKRVKIVGMILIQPFFGGEERRDSEIRFGRGYGLTLEMTDWFWK 240

Query: 241 AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           A+LP+GS+RDH AANVV  GS +SG+K+P ALVV GGLDLL DR +EY EWL+KSG+EVR
Sbjct: 241 AWLPVGSNRDHTAANVV--GSSISGVKVPAALVVIGGLDLLRDRNREYVEWLKKSGQEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 334
           VVEYPNG HGF    DLPEYSML+QDA++F  ++
Sbjct: 301 VVEYPNGTHGFIGKPDLPEYSMLIQDAKQFINKI 332

BLAST of CmaCh04G030200 vs. NCBI nr
Match: XP_022990625.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 685.3 bits (1767), Expect = 2.7e-193
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTRG 60
           MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTRG
Sbjct: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTRG 60

Query: 61  VASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDFA 120
           VASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDFA
Sbjct: 61  VASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDFA 120

Query: 121 REIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESAG 180
           REIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESAG
Sbjct: 121 REIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESAG 180

Query: 181 GNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKA 240
           GNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKA
Sbjct: 181 GNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKA 240

Query: 241 FLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRV 300
           FLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRV
Sbjct: 241 FLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRV 300

Query: 301 VEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 337
           VEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 VEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 336

BLAST of CmaCh04G030200 vs. NCBI nr
Match: XP_023549493.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 653.3 bits (1684), Expect = 1.1e-183
Identity = 319/333 (95.80%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTT-R 60
           MSSTGDTQSPSSSPNLPSKRKLADL +SVLFRH FRSDFTVNRG LRLLDRKVPPS+T R
Sbjct: 1   MSSTGDTQSPSSSPNLPSKRKLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTNR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNPPTCSESDR+PVIIYFHGGGFVFGSADSQQIDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPPTCSESDRVPVIIYFHGGGFVFGSADSQQIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESA 180
           AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSI+KSLPENADISRCFIAGESA
Sbjct: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISRCFIAGESA 180

Query: 181 GGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWK 240
           GGNIVHHVTVRAA+YEFKRLRIAGMIAIQPFLGGEERT+SEIRFGRGGGLTLE  DWYWK
Sbjct: 181 GGNIVHHVTVRAAEYEFKRLRIAGMIAIQPFLGGEERTKSEIRFGRGGGLTLEVTDWYWK 240

Query: 241 AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLE+SGKEVR
Sbjct: 241 AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLERSGKEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRR 333
           VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTR+
Sbjct: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRQ 333

BLAST of CmaCh04G030200 vs. NCBI nr
Match: KAG6602724.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033410.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 645.2 bits (1663), Expect = 3.1e-181
Identity = 320/337 (94.96%), Postives = 325/337 (96.44%), Query Frame = 0

Query: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPP-STTR 60
           M STGDTQSPSSSPNLPSKRKLADL +S+LFRH FRSDFTVNRG LRLLDRKVPP STTR
Sbjct: 1   MPSTGDTQSPSSSPNLPSKRKLADLLSSILFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNP TCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESA 180
           AREIGAIVVSVNY LAPEHRFPSPYDDGFRVLKAIDEGSI+KSLPENADIS CFIAGESA
Sbjct: 121 AREIGAIVVSVNYCLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180

Query: 181 GGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWK 240
           GGNIVHHVTVRAA+YEFKRLRIAGMIAIQPFLGGEERT+SEIRFGRGGGLTLE  DWYWK
Sbjct: 181 GGNIVHHVTVRAAEYEFKRLRIAGMIAIQPFLGGEERTKSEIRFGRGGGLTLEMTDWYWK 240

Query: 241 AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           AFLPIGSSRDHPAANVVR  SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR
Sbjct: 241 AFLPIGSSRDHPAANVVR--SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 337
           VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 335

BLAST of CmaCh04G030200 vs. NCBI nr
Match: XP_022964989.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 642.1 bits (1655), Expect = 2.6e-180
Identity = 317/337 (94.07%), Postives = 325/337 (96.44%), Query Frame = 0

Query: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPP-STTR 60
           M STGDTQSPSSSPNLPSKR+LADL +SVLFRH FRSDFTVNRG LRLLDRKVPP STTR
Sbjct: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNP TCSESDRMPVIIYFHGGGFVFGSADSQ+IDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESA 180
           AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSI+KSLPENADIS CFIAGESA
Sbjct: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180

Query: 181 GGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWK 240
           GGNIVHHVTVRAA+YEFKRL+IAGMIAIQPFLGGEERT+SEIRFG+GGGLTLE  DWYWK
Sbjct: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240

Query: 241 AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           AFLPIGSSRDHP ANVVR  SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR
Sbjct: 241 AFLPIGSSRDHPGANVVR--SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 337
           VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 335

BLAST of CmaCh04G030200 vs. NCBI nr
Match: XP_008441192.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo] >KAA0037036.1 putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK06614.1 putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 501.9 bits (1291), Expect = 4.3e-138
Identity = 248/328 (75.61%), Postives = 279/328 (85.06%), Query Frame = 0

Query: 1   MSSTGDTQSPSSS-PNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTTR 60
           M+S GD QSP+SS P LP  RKLADLFTS LFR  FRSDFTVNR LL LLDRKVPP TTR
Sbjct: 1   MASAGDAQSPNSSPPTLPWNRKLADLFTSFLFRRAFRSDFTVNRRLLGLLDRKVPPFTTR 60

Query: 61  GVASFDTTLDSSSSD-LWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRD 120
           GVA+ D  +DSSSSD LW+RVYNP T   SD +PVI++FHGGGFV+GSADS  ID+F RD
Sbjct: 61  GVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVYGSADSPPIDTFCRD 120

Query: 121 FAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGES 180
           FAREIGAIV+SVNYRLAPEHRFPS +DDGF VLKA++EG I+++LPENAD+ RCFIAGES
Sbjct: 121 FAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVISETLPENADLRRCFIAGES 180

Query: 181 AGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYW 240
           AGGNI HHVTVRAA+ E KRL+I GMI IQPFLGGEER  SEIRFGRG G+T+E ADW+W
Sbjct: 181 AGGNIAHHVTVRAAEGELKRLKIVGMILIQPFLGGEERRDSEIRFGRGYGMTVEMADWFW 240

Query: 241 KAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEV 300
           KA+LP+GS+RDHPAANVVR  S +SG+KLPPA+VV GGLDLL DR KEY EWL+K G+EV
Sbjct: 241 KAWLPVGSNRDHPAANVVR--SSISGVKLPPAVVVIGGLDLLRDRQKEYVEWLKKWGEEV 300

Query: 301 RVVEYPNGIHGFSWFSDLPEYSMLMQDA 327
           RVVEYPNG HGF W  DLPEYSMLMQDA
Sbjct: 301 RVVEYPNGTHGFIWKPDLPEYSMLMQDA 326

BLAST of CmaCh04G030200 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 267.3 bits (682), Expect = 1.7e-71
Identity = 144/328 (43.90%), Postives = 200/328 (60.98%), Query Frame = 0

Query: 10  PSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPS--TTRGVASFDTT 69
           P+    LP K ++A    S +  +  R D T+NR  LRL D + PP+      V++ D  
Sbjct: 7   PNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFV 66

Query: 70  LDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDFAREIGAIV 129
           +D  S DLW R+Y P      D++PV+++FHGGGF F S ++   D+  R FAR++ A V
Sbjct: 67  VD-QSRDLWFRLYTPHV--SGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYV 126

Query: 130 VSVNYRLAPEHRFPSPYDDGFRVLKAIDE--GSIAKSLPENADISRCFIAGESAGGNIVH 189
           +SVNYRLAPEHR+P+ YDDGF  LK I+E  GSI   LP NAD+SRCF AG+SAGGNI H
Sbjct: 127 ISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSI---LPANADLSRCFFAGDSAGGNIAH 186

Query: 190 HVTVRAA---KYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWKAFL 249
           +V +R     +  F  +++ G+I+IQPF GGEERT++E +      ++ +  DW WKA  
Sbjct: 187 NVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA-- 246

Query: 250 PIGSSRDHPAANVVRSGS-DVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV 309
            +G +RDH A NV    + D+SGL  P  +VV  G D L D  + Y EWL+  GK+  ++
Sbjct: 247 -MGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLI 306

Query: 310 EYPNGIHGFSWFSDLPEYSMLMQDAREF 330
           EYPN  H F  F +LPE   L+   ++F
Sbjct: 307 EYPNMFHAFYIFPELPEAGQLIMRIKDF 325

BLAST of CmaCh04G030200 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 214.2 bits (544), Expect = 1.7e-55
Identity = 131/341 (38.42%), Postives = 182/341 (53.37%), Query Frame = 0

Query: 11  SSSPNLPSKRKLADLFTSVL-------FRHVFRSDFTVNRGLLRLLDRKVPPST--TRGV 70
           S   NL   + +  L T VL       +  + R D T NR L   LDRKVP +     GV
Sbjct: 4   SEEVNLIESKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGV 63

Query: 71  ASFDTTLDSSSSDLWLRVYNPPTCSES------------DRMPVIIYFHGGGFVFGSADS 130
            SFD  +D   ++L  RVY P     S            + +PVI++FHGG F   SA+S
Sbjct: 64  FSFDVIID-RQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANS 123

Query: 131 QQIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADI 190
              D+  R      GA+VVSVNYR APE+R+P  YDDG+ VLK ++  S  +S  ++   
Sbjct: 124 AIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNSSSWLRSKKDSK-- 183

Query: 191 SRCFIAGESAGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGL 250
            R F+AG+S+GGNIVH+V VRA +    R+ + G I + P  GG ERT+SE R      +
Sbjct: 184 VRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFV 243

Query: 251 TLETADWYWKAFLPIGSSRDHPAANVVRSGS-DVSGLKLPPALVVNGGLDLLSDRIKEYC 310
           T+   DWYW+AFLP G  R+HPA +     S  + GL  P +LVV  GLDL+ D   +Y 
Sbjct: 244 TVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYA 303

Query: 311 EWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 330
           E L+K+G+EV+++       GF    +   +  +M +   F
Sbjct: 304 EGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAF 338

BLAST of CmaCh04G030200 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-51
Identity = 120/344 (34.88%), Postives = 182/344 (52.91%), Query Frame = 0

Query: 15  NLPSKRKLADLFTSVL-------FRHVFRSDFTVNRGLLRLLDRKVPPST--TRGVASFD 74
           NL   +++  L T VL       ++ + R D + NR L   LDRKVP ++    GV SFD
Sbjct: 8   NLNECKRIVPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD 67

Query: 75  TTLDSSSSDLWLRVYNPPT---------------CSESDRMPVIIYFHGGGFVFGSADSQ 134
                S+++L  R+Y P +                S ++ +PV+I+FHGG F   SA+S 
Sbjct: 68  HV--DSTTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSA 127

Query: 135 QIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADIS 194
             D+F R      G +VVSV+YR +PEHR+P  YDDG+  L  +      +S  ++    
Sbjct: 128 IYDTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSN--V 187

Query: 195 RCFIAGESAGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLT 254
             ++AG+S+GGNI H+V VRA     + +++ G I + P  GG+ERTQSE        +T
Sbjct: 188 YVYLAGDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVT 247

Query: 255 LETADWYWKAFLPIGSSRDHPAANVV-RSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCE 314
           ++  DWYW+A+LP G  RDHPA N     G  + G+  P +LVV  GLDL+ D    Y +
Sbjct: 248 IQDRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVD 307

Query: 315 WLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 334
            L+K+G EV ++       GF +  +   +  LM++  +F   +
Sbjct: 308 GLKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

BLAST of CmaCh04G030200 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 8.8e-49
Identity = 123/343 (35.86%), Postives = 170/343 (49.56%), Query Frame = 0

Query: 11  SSSPNLPSKRKLADLFTSVL-------FRHVFRSDFTVNRGLLRLLDRKVPPST--TRGV 70
           S   NL   R +  L T VL       +  + R D T NR L   LDRKV  +     GV
Sbjct: 4   SDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGV 63

Query: 71  ASFDTTLDSSSSDLWLRVYNPPTCS--------------ESDRMPVIIYFHGGGFVFGSA 130
            SFD  +D    +L  RVY P                  + D +PVI++FHGG F   SA
Sbjct: 64  FSFDVLID-RRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSA 123

Query: 131 DSQQIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENA 190
           +S   D+  R        +VVSVNYR APE+ +P  YDDG+  L  ++  S  KS  ++ 
Sbjct: 124 NSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSK 183

Query: 191 DISRCFIAGESAGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGG 250
                F+AG+S+GGNI H+V +RA +     + + G I + P  GG ERT+SE       
Sbjct: 184 --VHIFLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKY 243

Query: 251 GLTLETADWYWKAFLPIGSSRDHPAANVVR-SGSDVSGLKLPPALVVNGGLDLLSDRIKE 310
            +T+   DWYWKAFLP G  R+HPA N     G  + G+  P +LVV  GLDL+ D    
Sbjct: 244 FVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLA 303

Query: 311 YCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 330
           Y E L+K+G+EV+++       GF    +   +  +M +   F
Sbjct: 304 YAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAF 340

BLAST of CmaCh04G030200 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 4.5e-45
Identity = 102/280 (36.43%), Postives = 155/280 (55.36%), Query Frame = 0

Query: 48  LLDRKVPPSTTRGVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSA 107
           L+ +++P    + V  F  ++    ++L LR+Y P + S    +PV+++FHGGGF FGS 
Sbjct: 34  LITQQIPFKNNQTVL-FKDSIYHKPNNLHLRLYKPISASNRTALPVVVFFHGGGFCFGSR 93

Query: 108 DSQQIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPE-- 167
                 +F    A  + A+VVS +YRLAPEHR P+ ++D   VL  + + +++  +    
Sbjct: 94  SWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWF 153

Query: 168 ----NADISRCFIAGESAGGNIVHHVTVR--AAKYEFKRLRIAGMIAIQPFLGGEERTQS 227
               + D  R F+ G+S+GGNI H + VR  +   E   +R+ G + + PF GGEERT S
Sbjct: 154 EDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSIELTPVRVRGYVLMGPFFGGEERTNS 213

Query: 228 EIRFGRGGGLTLETADWYWKAFLPIGSSRDHPAANVVRSGS-DVSGLKLPPALVVNGGLD 287
           E        L+L+  D +W+  LP G++RDH  AN     S  +  + L P LV+ GG +
Sbjct: 214 E-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMANPFGPTSPTLESISLEPMLVIVGGSE 273

Query: 288 LLSDRIKEYCEWLEK-SGKEVRVVEYPNGIHGFSWFSDLP 318
           LL DR KEY   L+K  GK V  +E+ N  HGF  +S+ P
Sbjct: 274 LLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF--YSNYP 309

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT102.3e-7043.90Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G62.3e-5438.42Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5453.2e-5137.74Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9LYC11.6e-5034.88Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Q9MAA71.2e-4735.86Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JQL41.3e-193100.00probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111487452 PE=4 SV... [more]
A0A6J1HPS21.3e-18094.07probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111464932 PE=4 ... [more]
A0A5A7T4I62.1e-13875.61Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3B2W22.1e-13875.61probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103485400 PE=4 SV=1[more]
A0A0A0LMQ64.6e-13872.75Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G4014... [more]
Match NameE-valueIdentityDescription
XP_022990625.12.7e-193100.00probable carboxylesterase 18 [Cucurbita maxima][more]
XP_023549493.11.1e-18395.80probable carboxylesterase 18 [Cucurbita pepo subsp. pepo][more]
KAG6602724.13.1e-18194.96putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022964989.12.6e-18094.07probable carboxylesterase 18 [Cucurbita moschata][more]
XP_008441192.14.3e-13875.61PREDICTED: probable carboxylesterase 18 [Cucumis melo] >KAA0037036.1 putative ca... [more]
Match NameE-valueIdentityDescription
AT5G23530.11.7e-7143.90carboxyesterase 18 [more]
AT5G27320.11.7e-5538.42alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.11.1e-5134.88alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.18.8e-4935.86alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.14.5e-4536.43alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 5..334
e-value: 8.6E-86
score: 290.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 54..334
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 94..310
e-value: 1.0E-59
score: 201.9
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 12..332
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 12..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G030200.1CmaCh04G030200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity