CmaCh04G030060 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G030060
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein disulfide-isomerase 5-2-like
LocationCma_Chr04: 19655851 .. 19659730 (+)
RNA-Seq ExpressionCmaCh04G030060
SyntenyCmaCh04G030060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAAAACATTTTGGAAATGAGAAAATAAAAGAAATGAATTAAAACGAAATTGTTTTGGAGGCAGTTCTGTCTTCGGAAGTGATTTCGAAGACCTCACAAATCAAAATCTGCTTGCTTAGAAATGAATCAAACTTGCAACTTCGGCAGTACTATGAGCAGCTGATAAACGAAGAAGAAGAAATAAATCGAAACCACGAGAGCGATCGAGATCGTGATCCTCATCAAATTGAACACTCTGAAGATGCAGAGATCGTGGCAAGCGGTGGTTATTGTCCTCGTTTCAATCTTAGCACTGCCATCTTCTTCCTTTTCGAGCTCGGAGTTGCCACTCACGGCGGATGGAACGGTGTTGGAGCTCGACGAATCCAATTTCGATCTCGCCATTTCTTCATTTGATTACATATTGGTCGACTTTTACGCCCCTTGGTGCGGCCACTGCAAGCGTCTTTCTCCAGAGGTACCTTCGTCCCCTCATATTTTCTCTTCCATTGTTCTAGATTATTCGTCGGATTCGCACTTTTAGTTACATTGTTTCTGTTATCATTTGATCTCCCTTTGGATACGTAGATGGGTGAAACAAAGTAATTTTTATTAAAACCTTAATATGGATGTGTAGAGCCAACTAAACAGAATCGAATAGGACTTCAAGTACTTATTTGTGTATTGAATCAGTTCGTCAATAATATGGAGGTTGTGACATATATGTTTTCCGATTGAACTGTCTTCGTAGCGATGAGGATTTTGAATATATGGATGTGCTAAGTCGGCTTCCATCTGGTCAAATTGAGTGGCCTAATTTCATCTTCAGGAGAATCTCAATTGTAAAGAGATTTCTGATCTGGATTGTTTGTTTCTTCAGTCTTGTGGTGTGAGTTTCACTTTCAAATTCGTTGTGGAATTTTCATAATATTCAAATAAAATTTGGAGGAGTTTCCTGTAAAGTTGGCTCTCACTTCATATCGAGGGAGATGATTCTTCGAAAGAGTAGAGGTTTCTTTAATAAATTTGTTCGTGCATCAATGATTCACAGTTTGTAGATTTTGACGCTTGGCTTTGGGTTACTATTACCAATAAGCTTCAAAATCTCTCTATTTTCTTCCATTGTCAACAATTTGATAGTTTACTTGTAAAGTTCTTTCCGGATGAGGTGTTCTTGGTTCTTGCTCCGTTTAGCACGGTACATTATTTGGACTGCAGCTGTTATTCTGCAGCCATTCTATTGCATGTGATTTTCCATATTCTTTTTAATGTGCTGAATGCTGTTGGAGTCTCTTGGAACTTGTCGTCGGGCTAAAATACAGATATCGCACCCTGCAGTGCGGTCTATTATGCACAAAATAAGTATTATTTCCTTTGGTTAATTATCTTTTTTAATTGTGTAGTTGGACGCTGCTGCGCCTCAGCTTGCCAAATTGAAGGAGCCTATAGTTCTTGCAAAACTGAATGCTGACAAGTTTACTCGTCTTGCTAAAAAATATGACGTTGAGTATGTTATTTCCTTTTATTCACATCTCCGATGTAGTAAACAATGTGGGTTTATTTTCAATAGGCTTGGAGTTTGGTTGTGTCTCTTAGATTAGATGCATAGTTTTAGTGCGAATCTCCAAGAGACCATTTCCATTATTAATGGGGAGTTTGCTACTGAATACCAGTTCTTGAATTATATTCGAAAGGCAAACTATGATTCTCTTCTAACTCCACAAACGTTTTTTTAAATTGTAGTGCCTATCCTACAATCAAGATCTTCATGCATGGCGTTCCAGTAGACTATTATGGACCAAGGAAAGCAGAGTTACTTGTCCGTTATTTGAAGAAATTTGTTGCCCCGGATGTCTCAATTCTCGAGTCAGACTCGTCGATTAATGAATTTGTTGAAGCTTCTGGGCCTTACTTCCCTATTTATTTGGGTTTCGGTTTAGATGAATCTATTATTTCTAAATTTGCTATCAAGTACAAGAAGAAAGCTTGGTTTTCAGTGGCCAAGGGATTCTCGGAGGATATCATGGTATCTTATGATTTTGATAAAGTCCCTTCCTTGGTTTCTATCCATCCAAATTACAACAATGAACGGAGCATTTTTTATGGTCCCTTTGAAGGTTGGTTTGTCTATCAACTATTACCTTGCATGTGTTTCACATCCACGTGTCATCAGAATGGATTGACAGAGTAAAAACCTTGTTATGCAGGATGCTATACCTAGTATAAACTGTTTAAGGGGCTAAGTTCCCAACTGCTTGAATTCTTATCTGTTACTTGTTTACACCAAGATGTCTGGTTGAGTTTTTATCCATTGCCAGGAATATCCCAATATCATTTCTTCTGTATATAGTTGTGTATATGCCCTTTAGGTGCTTGAATGTTGATTCGTCTCTCCATCATCGATATGTTATGCATGCTAGATAAAATTGTTTAAGCCTTCCTATTTTACTTGTGCAGATAAATTCTTGGAGGAGTTCATAAAACAAAGCTTGTTCCCTCTTGTTCTGCCCATAAACTACGACACACTTAAGCTACTAAAGGACGATGAGAGGAAAATAGCACTCACAATAGTGAAGGATGAGGATGATGACCAGACAAAGAATTTGATCAATTTATTAAAGGCTGCTGCATCTGCTAATCGTGACTTGGTGTTTGCTTATGTCGGGGCTAAACAGTGGGGAGAATTTGCTGATTCATTTGGGGACAAGAAGACCACATTACCAAAAATGATCATTTGGGATGGGGAGGAGGAATACTTAATGGTAAGTCCCTCATGAAGGCAAATGATACTGTATAGCATGTTCATTCAATCATCTACATTTAGATTTTTCTCTGTTACGATGTCACACTACTAGGACTTTGTCTGAGTGGATATGCTCACAATTTTTGTACTGCCGTAGGGTAATAAAACTCGTGACAAACTAAAGTTTCTTGAATATTTGTCTTAAAATTCACCCTGAATATTAAACCTTCGAGCATGTTATTGTTTTTAGTTAGGAATAGCGACAAACATGCATGCATGCCCAACTATGAGTATAACTGGACATGTATATATGATAGCATTCAATCACTCATGCATGTACTGAGTCTGAAGAACAAGTTTTCTTTAATGGGTAGAAAGATTATGAAATTAGTCAAGGTGTGCATAAACTGGCCCGAACACTCACAAATATAAAATAAAAGATGCGTGAATGTATGTACATATCATACTGAGGTAGTTTTTCTGTTTTTCTGTTTGATAGGTTATTGGTTCAGAAAGCATCATTGGGAACGACCATGCATCCGAAATTTCTAAATTTGTTGAAGGATATAGGGAAGGGAGAACTGAAGAAAGAAGAGTTGCAGGTCCTTCATTTATTGGCTTCATAAATTCAATGATAGGCACCTGGAGTGTATACATCATTGTATTTATAGTTGCTATAGTAATGCTTTTCCAATACCTCACGAAAGATGATAACGAATATCATAGAGTCGATACTCCTGCTCAGGATCAGGTTGACCAAATTAGTGGCAGCTCTACCTCACAGGTTGAAAGGACTGAGTACAGGGCTGGTGACAAGGAAGATTGAATGTGAAATTTGGTGAATTAATTATTAGCATGACCTTTGAACGGCTTAGGGAAGCAGATATTGACAAGAATCAGACATAAAAGTATCTTGGGAGCCATAAAATAACTGATGAAATGTGTATTTTGGTGATCTGTTCCCAAGCGCTTTACCCCTTGCGAAAGTGAACTTATATTTGATGTATGCTTTTTATGCTTTACTTTGTAACAGTTAGGTTTCTGGATCAATCGAATTGAATTGACTTCTGCAGATAGATATTGTTCATGTTGGCTCTTTTAGTTGGAAGAATTGACTTATGCAGATAGATACTGCTCATGTTGGCTCTTTTAGTTGGA

mRNA sequence

CTAAAACATTTTGGAAATGAGAAAATAAAAGAAATGAATTAAAACGAAATTGTTTTGGAGGCAGTTCTGTCTTCGGAAGTGATTTCGAAGACCTCACAAATCAAAATCTGCTTGCTTAGAAATGAATCAAACTTGCAACTTCGGCAGTACTATGAGCAGCTGATAAACGAAGAAGAAGAAATAAATCGAAACCACGAGAGCGATCGAGATCGTGATCCTCATCAAATTGAACACTCTGAAGATGCAGAGATCGTGGCAAGCGGTGGTTATTGTCCTCGTTTCAATCTTAGCACTGCCATCTTCTTCCTTTTCGAGCTCGGAGTTGCCACTCACGGCGGATGGAACGGTGTTGGAGCTCGACGAATCCAATTTCGATCTCGCCATTTCTTCATTTGATTACATATTGGTCGACTTTTACGCCCCTTGGTGCGGCCACTGCAAGCGTCTTTCTCCAGAGTTGGACGCTGCTGCGCCTCAGCTTGCCAAATTGAAGGAGCCTATAGTTCTTGCAAAACTGAATGCTGACAAGTTTACTCGTCTTGCTAAAAAATATGACGTTGATGCCTATCCTACAATCAAGATCTTCATGCATGGCGTTCCAGTAGACTATTATGGACCAAGGAAAGCAGAGTTACTTGTCCGTTATTTGAAGAAATTTGTTGCCCCGGATGTCTCAATTCTCGAGTCAGACTCGTCGATTAATGAATTTGTTGAAGCTTCTGGGCCTTACTTCCCTATTTATTTGGGTTTCGGTTTAGATGAATCTATTATTTCTAAATTTGCTATCAAGTACAAGAAGAAAGCTTGGTTTTCAGTGGCCAAGGGATTCTCGGAGGATATCATGGTATCTTATGATTTTGATAAAGTCCCTTCCTTGGTTTCTATCCATCCAAATTACAACAATGAACGGAGCATTTTTTATGGTCCCTTTGAAGATAAATTCTTGGAGGAGTTCATAAAACAAAGCTTGTTCCCTCTTGTTCTGCCCATAAACTACGACACACTTAAGCTACTAAAGGACGATGAGAGGAAAATAGCACTCACAATAGTGAAGGATGAGGATGATGACCAGACAAAGAATTTGATCAATTTATTAAAGGCTGCTGCATCTGCTAATCGTGACTTGGTGTTTGCTTATGTCGGGGCTAAACAGTGGGGAGAATTTGCTGATTCATTTGGGGACAAGAAGACCACATTACCAAAAATGATCATTTGGGATGGGGAGGAGGAATACTTAATGGTTATTGGTTCAGAAAGCATCATTGGGAACGACCATGCATCCGAAATTTCTAAATTTGTTGAAGGATATAGGGAAGGGAGAACTGAAGAAAGAAGAGTTGCAGGTCCTTCATTTATTGGCTTCATAAATTCAATGATAGGCACCTGGAGTGTATACATCATTGTATTTATAGTTGCTATAGTAATGCTTTTCCAATACCTCACGAAAGATGATAACGAATATCATAGAGTCGATACTCCTGCTCAGGATCAGGTTGACCAAATTAGTGGCAGCTCTACCTCACAGGTTGAAAGGACTGAGTACAGGGCTGGTGACAAGGAAGATTGAATGTGAAATTTGGTGAATTAATTATTAGCATGACCTTTGAACGGCTTAGGGAAGCAGATATTGACAAGAATCAGACATAAAAGTATCTTGGGAGCCATAAAATAACTGATGAAATGTGTATTTTGGTGATCTGTTCCCAAGCGCTTTACCCCTTGCGAAAGTGAACTTATATTTGATGTATGCTTTTTATGCTTTACTTTGTAACAGTTAGGTTTCTGGATCAATCGAATTGAATTGACTTCTGCAGATAGATATTGTTCATGTTGGCTCTTTTAGTTGGAAGAATTGACTTATGCAGATAGATACTGCTCATGTTGGCTCTTTTAGTTGGA

Coding sequence (CDS)

ATGCAGAGATCGTGGCAAGCGGTGGTTATTGTCCTCGTTTCAATCTTAGCACTGCCATCTTCTTCCTTTTCGAGCTCGGAGTTGCCACTCACGGCGGATGGAACGGTGTTGGAGCTCGACGAATCCAATTTCGATCTCGCCATTTCTTCATTTGATTACATATTGGTCGACTTTTACGCCCCTTGGTGCGGCCACTGCAAGCGTCTTTCTCCAGAGTTGGACGCTGCTGCGCCTCAGCTTGCCAAATTGAAGGAGCCTATAGTTCTTGCAAAACTGAATGCTGACAAGTTTACTCGTCTTGCTAAAAAATATGACGTTGATGCCTATCCTACAATCAAGATCTTCATGCATGGCGTTCCAGTAGACTATTATGGACCAAGGAAAGCAGAGTTACTTGTCCGTTATTTGAAGAAATTTGTTGCCCCGGATGTCTCAATTCTCGAGTCAGACTCGTCGATTAATGAATTTGTTGAAGCTTCTGGGCCTTACTTCCCTATTTATTTGGGTTTCGGTTTAGATGAATCTATTATTTCTAAATTTGCTATCAAGTACAAGAAGAAAGCTTGGTTTTCAGTGGCCAAGGGATTCTCGGAGGATATCATGGTATCTTATGATTTTGATAAAGTCCCTTCCTTGGTTTCTATCCATCCAAATTACAACAATGAACGGAGCATTTTTTATGGTCCCTTTGAAGATAAATTCTTGGAGGAGTTCATAAAACAAAGCTTGTTCCCTCTTGTTCTGCCCATAAACTACGACACACTTAAGCTACTAAAGGACGATGAGAGGAAAATAGCACTCACAATAGTGAAGGATGAGGATGATGACCAGACAAAGAATTTGATCAATTTATTAAAGGCTGCTGCATCTGCTAATCGTGACTTGGTGTTTGCTTATGTCGGGGCTAAACAGTGGGGAGAATTTGCTGATTCATTTGGGGACAAGAAGACCACATTACCAAAAATGATCATTTGGGATGGGGAGGAGGAATACTTAATGGTTATTGGTTCAGAAAGCATCATTGGGAACGACCATGCATCCGAAATTTCTAAATTTGTTGAAGGATATAGGGAAGGGAGAACTGAAGAAAGAAGAGTTGCAGGTCCTTCATTTATTGGCTTCATAAATTCAATGATAGGCACCTGGAGTGTATACATCATTGTATTTATAGTTGCTATAGTAATGCTTTTCCAATACCTCACGAAAGATGATAACGAATATCATAGAGTCGATACTCCTGCTCAGGATCAGGTTGACCAAATTAGTGGCAGCTCTACCTCACAGGTTGAAAGGACTGAGTACAGGGCTGGTGACAAGGAAGATTGA

Protein sequence

MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYAPWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVPVDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKFAIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYVGAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGRTEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQISGSSTSQVERTEYRAGDKED
Homology
BLAST of CmaCh04G030060 vs. ExPASy Swiss-Prot
Match: Q94F09 (Protein disulfide-isomerase 5-2 OS=Arabidopsis thaliana OX=3702 GN=PDIL5-2 PE=1 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 7.8e-151
Identity = 268/437 (61.33%), Postives = 349/437 (79.86%), Query Frame = 0

Query: 8   VVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYAPWCGHCK 67
           +++  +S L L  S  +SS+   T DGTVLEL +SNFD AIS+FD I VDFYAPWCGHCK
Sbjct: 6   LLLCWISFLTLSISISASSDDQFTLDGTVLELTDSNFDSAISTFDCIFVDFYAPWCGHCK 65

Query: 68  RLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVPVDYYGPR 127
           RL+PELDAAAP LAKLK+PIV+AKLNADK++RLA+K ++DA+PT+ ++ HGVP++YYGPR
Sbjct: 66  RLNPELDAAAPILAKLKQPIVIAKLNADKYSRLARKIEIDAFPTLMLYNHGVPMEYYGPR 125

Query: 128 KAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKFAIKYKKK 187
           KA+LLVRYLKKFVAPDV++LESDS++ EFVE +G +FP+++GFGL+ESIIS    KYKKK
Sbjct: 126 KADLLVRYLKKFVAPDVAVLESDSTVKEFVEDAGTFFPVFIGFGLNESIISGLGRKYKKK 185

Query: 188 AWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLFPLV 247
           AWF+V+K  SED MVSYDFDK P+LV+ HP Y NE S+FYGPFED FLEEF+KQS  PL+
Sbjct: 186 AWFAVSKEVSEDTMVSYDFDKAPALVANHPTY-NEHSVFYGPFEDGFLEEFVKQSFLPLI 245

Query: 248 LPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYVGAKQWGE 307
           LPIN+DTLKLLKDDERKI LTIV+DE  +  + L   L+AAA ANRDLVF YVG KQ+ E
Sbjct: 246 LPINHDTLKLLKDDERKIVLTIVEDETHESLEKLYKALRAAAHANRDLVFGYVGVKQFEE 305

Query: 308 FADSFG-DKKTTLPKMIIWDGEEEYLMVIGSESII-GNDHASEISKFVEGYREGRTEERR 367
           F DSF  DKKT LPK+++WDG+EEY  V G E+I    DH +++S+F+EGYREGRTE+++
Sbjct: 306 FVDSFHVDKKTNLPKIVVWDGDEEYDQVTGIETITQEEDHLTQVSRFLEGYREGRTEKKK 365

Query: 368 VAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTK-DDNEYHRVDTPAQDQVDQISGS 427
           + GPSF+GFINSMIG  SVYI+VF+VA++M+ + L + ++    R  T  +++VDQ   +
Sbjct: 366 INGPSFMGFINSMIGIRSVYILVFLVAVIMMLRSLGQVEEPTGVRTATAVRERVDQ--AT 425

Query: 428 STSQVERTEYRAGDKED 442
           +  + E +E++  DK++
Sbjct: 426 TVPEDESSEHKPSDKKE 439

BLAST of CmaCh04G030060 vs. ExPASy Swiss-Prot
Match: Q0JD42 (Protein disulfide isomerase-like 5-2 OS=Oryza sativa subsp. japonica OX=39947 GN=PDIL5-2 PE=2 SV=2)

HSP 1 Score: 470.7 bits (1210), Expect = 1.8e-131
Identity = 222/396 (56.06%), Postives = 311/396 (78.54%), Query Frame = 0

Query: 11  VLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYAPWCGHCKRLS 70
           +L+ +L+  +++ +++E     DG V+ELDES+F+ A+ + DY+ VDFYAPWCGHCKRL+
Sbjct: 20  LLLLLLSFHAAAAAAAE-EFPRDGRVIELDESSFEAALGAIDYLFVDFYAPWCGHCKRLA 79

Query: 71  PELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVPVDYYGPRKAE 130
           PELD AAP LA L EPI++AK+NADK+ +L  KY VD +PT+ +F+HGVP++Y G RKA+
Sbjct: 80  PELDEAAPVLAGLSEPIIVAKVNADKYRKLGSKYGVDGFPTLMLFIHGVPIEYTGSRKAD 139

Query: 131 LLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKFAIKYKKKAWF 190
           LLVR L KFVAPDVSILESDS+I  FVE +G  FP+++GFG++ES+I+ +  KYKK+AWF
Sbjct: 140 LLVRNLNKFVAPDVSILESDSAIKSFVENAGTSFPMFIGFGVNESLIAGYGGKYKKRAWF 199

Query: 191 SVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLFPLVLPI 250
           +VAK FSED MV+YDFDKVP+LVS+HP Y  E+S+FYGPFE  FLE+FI+QSL PL +PI
Sbjct: 200 AVAKDFSEDFMVTYDFDKVPALVSLHPKY-KEQSVFYGPFEGSFLEDFIRQSLLPLTVPI 259

Query: 251 NYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYVGAKQWGEFAD 310
           N +TLK+L DD+RK+ L I++D+ D+ +  L+ +L++AA+ANRDLVF YVG KQW EF +
Sbjct: 260 NTETLKMLDDDDRKVVLAILEDDSDETSSQLVKVLRSAANANRDLVFGYVGIKQWDEFVE 319

Query: 311 SFG-DKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGRTEERRVAGP 370
           +F   K + LPK+I+WD  EEY +V GSE +   D AS+IS+F+EGYR GRT +++V+GP
Sbjct: 320 TFDISKSSQLPKLIVWDRNEEYEVVEGSEKLEEGDQASQISQFLEGYRAGRTTKKKVSGP 379

Query: 371 SFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDN 406
           SF+GF+NS++   S+YI++ + A++ +  Y T  D+
Sbjct: 380 SFMGFLNSLVSLNSLYILICVFALLGVMIYFTGQDD 413

BLAST of CmaCh04G030060 vs. ExPASy Swiss-Prot
Match: Q0E0I1 (Protein disulfide isomerase-like 5-3 OS=Oryza sativa subsp. japonica OX=39947 GN=PDIL5-3 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 8.5e-121
Identity = 219/412 (53.16%), Postives = 299/412 (72.57%), Query Frame = 0

Query: 8   VVIVLVSILALPSSSF--------SSSELPLTADGTVLELDESNFDLAISSFDYILVDFY 67
           VV++L+ ++ LP+++          + E  +  DG VLELD+ NFD A+ +   + VDFY
Sbjct: 12  VVVLLLLVVVLPATTCGADAGGGGEAEEFQIPRDGRVLELDDGNFDAAVRAAGLLFVDFY 71

Query: 68  APWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGV 127
           APWCGHCKRL+P+LD AAP LA L  PIV+AK+NADK+ +L  KY VD +PT+ +F HG 
Sbjct: 72  APWCGHCKRLAPQLDEAAPVLAGLSTPIVVAKVNADKYKKLGSKYGVDGFPTLMLFDHGT 131

Query: 128 PVDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISK 187
           P +Y G RKA+LLV  LKK VAPDVS+LESDS+I  FVE +G  FP++LGFG+DES+I +
Sbjct: 132 PTEYTGSRKADLLVENLKKLVAPDVSVLESDSAIKSFVEDAGMGFPLFLGFGVDESLIVE 191

Query: 188 FAIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFED-KFLEEF 247
           +  KYK +AWFSVAK FSED+MV YDFDKVP+LVS++P Y  E+SIFYGPF+D  FLE+F
Sbjct: 192 YGAKYKNRAWFSVAKDFSEDMMVFYDFDKVPALVSVNPKY-REQSIFYGPFDDGAFLEDF 251

Query: 248 IKQSLFPLVLPINYDTLKLLKDDERKIALTIVK-DEDDDQTKNLINLLKAAASANRDLVF 307
           I+ SL PLV+P+N +T+K+L DD RK+ L I++ DE D+ +  LI +L++AASANRDLVF
Sbjct: 252 IRNSLLPLVVPMNRETVKMLNDDGRKVVLMILQDDESDENSPRLIKVLRSAASANRDLVF 311

Query: 308 AYVGAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYR 367
            YVG  QW EF ++F  K + LP MI+WD +EEY +V GSE +   D+ S+IS+F+EGYR
Sbjct: 312 GYVGVNQWEEFTETFDVKSSELPTMIVWDKKEEYEIVEGSERLEEGDYGSQISRFLEGYR 371

Query: 368 EGRTEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHR 410
            GRT +++V   S      +++G  +VYI+VF+VA+++L  Y +    E  R
Sbjct: 372 AGRTIKKKVGDRS-----PTLLGVNAVYILVFLVAVLVLLMYFSGQGEEDQR 417

BLAST of CmaCh04G030060 vs. ExPASy Swiss-Prot
Match: P05307 (Protein disulfide-isomerase OS=Bos taurus OX=9913 GN=P4HB PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 8.4e-28
Identity = 107/336 (31.85%), Postives = 161/336 (47.92%), Query Frame = 0

Query: 36  VLELDESNFDLAISSFDYILVDFYAPWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNAD 95
           VL L + NFD A+++  Y+LV+FYAPWCGHCK L+PE   AA +L      I LAK++A 
Sbjct: 28  VLVLHKGNFDEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDAT 87

Query: 96  KFTRLAKKYDVDAYPTIKIFMHG---VPVDYYGPRKAELLVRYLKKFVAPDVSILESDSS 155
           + + LA++Y V  YPTIK F +G    P +Y   R+A+ +V +LKK   P  S L   ++
Sbjct: 88  EESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAASTLSDGAA 147

Query: 156 INEFVEASGPYFPIYLGFGLD-ESIISK---FAIKYKKKAWFSVAKGFSEDIMVSYDFDK 215
               VE+S       +GF  D ES  +K    A +      F +    + D+   Y  DK
Sbjct: 148 AEALVESSE---VAVIGFFKDMESDSAKQFFLAAEVIDDIPFGITS--NSDVFSKYQLDK 207

Query: 216 VPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLFPLVLPINYDTLKLLKDDERK--IA 275
               V +   ++  R+ F G    + L +FIK +  PLV+     T   +   E K  I 
Sbjct: 208 --DGVVLFKKFDEGRNNFEGEVTKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHIL 267

Query: 276 LTIVKDEDDDQTKNLINLLKAAASANRDLVFAYVGAKQWG--EFADSFGDKKTTLPKMII 335
           L + K   D + K L N  KAA S    ++F ++ +         + FG KK   P + +
Sbjct: 268 LFLPKSVSDYEGK-LSNFKKAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRL 327

Query: 336 WDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 361
              EEE          +    A +I++F   + EG+
Sbjct: 328 ITLEEEMTKYKPESDEL---TAEKITEFCHRFLEGK 352

BLAST of CmaCh04G030060 vs. ExPASy Swiss-Prot
Match: P54399 (Protein disulfide-isomerase OS=Drosophila melanogaster OX=7227 GN=Pdi PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.1e-27
Identity = 93/344 (27.03%), Postives = 167/344 (48.55%), Query Frame = 0

Query: 24  SSSELPLTADGTVLELDESNFDLAISSFDYILVDFYAPWCGHCKRLSPELDAAAPQLAKL 83
           +S+E  +  +  VL     NF   I+  +++LV+FYAPWCGHCK L+PE   AA QLA+ 
Sbjct: 17  ASAEAEVKVEEGVLVATVDNFKQLIADNEFVLVEFYAPWCGHCKALAPEYAKAAQQLAEK 76

Query: 84  KEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVPVDYYGPRKAELLVRYLKKFVAPD 143
           + PI LAK++A     LA++Y V  YPT+K F  G PV+Y G R+A  ++ ++ K   P 
Sbjct: 77  ESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSGSPVEYSGGRQAADIIAWVTKKTGPP 136

Query: 144 VSILESDSSINEFVEASGPYFPIYLGFGLD-ESIISKFAIKYKKKAWFSVAKGFSEDIMV 203
              L S +   +F++ +       +GF  D ES  +K   K    A  S   G S +  V
Sbjct: 137 AKDLTSVADAEQFLKDNE---IAIIGFFKDLESEEAKTFTKV-ANALDSFVFGVSSNADV 196

Query: 204 SYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLFPLVLPINYDTLKLLKDDE 263
              ++   + V +   +++++S+F G   ++ L++F +    PL++  N+++   +    
Sbjct: 197 IAKYEAKDNGVVLFKPFDDKKSVFEGELNEENLKKFAQVQSLPLIVDFNHESASKIFGGS 256

Query: 264 RKIALTIVKDEDDDQTKNLINLLKAAASANR-DLVFAYVGA--KQWGEFADSFGDKKTTL 323
            K  L      +    +  ++ LK  A   R D++F  + +  +      + FG  K  +
Sbjct: 257 IKSHLLFFVSREGGHIEKYVDPLKEIAKKYRDDILFVTISSDEEDHTRIFEFFGMNKEEV 316

Query: 324 PKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGRTEE 364
           P + +   EE+          +    A  I  F++ + +G+ ++
Sbjct: 317 PTIRLIKLEEDMAKYKPESDDLS---AETIEAFLKKFLDGKLKQ 353

BLAST of CmaCh04G030060 vs. ExPASy TrEMBL
Match: A0A6J1JQY3 (protein disulfide-isomerase 5-2-like OS=Cucurbita maxima OX=3661 GN=LOC111487041 PE=4 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 3.8e-249
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
           AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ
Sbjct: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420

Query: 421 ISGSSTSQVERTEYRAGDKED 442
           ISGSSTSQVERTEYRAGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 441

BLAST of CmaCh04G030060 vs. ExPASy TrEMBL
Match: A0A6J1H1U7 (protein disulfide-isomerase 5-2-like OS=Cucurbita moschata OX=3662 GN=LOC111459596 PE=4 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 1.5e-245
Identity = 433/441 (98.19%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYIL+DFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILIDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
           AIKYKKKAWFSVAKGFSEDIMVSYDFDKVP+LVSIHPNYNNERSIFYGPFEDKFLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMIIWDG+EEYLMVIGSESIIGNDHASEISKFV+GYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGKEEYLMVIGSESIIGNDHASEISKFVQGYREGR 360

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TEERRVAGPSFIGFINSMIGT SVYIIVFIVA++MLFQYLTKDDNEYHRVDTPA+DQVDQ
Sbjct: 361 TEERRVAGPSFIGFINSMIGTKSVYIIVFIVAVIMLFQYLTKDDNEYHRVDTPARDQVDQ 420

Query: 421 ISGSSTSQVERTEYRAGDKED 442
           ISGSSTSQVERTEYRAGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 441

BLAST of CmaCh04G030060 vs. ExPASy TrEMBL
Match: A0A6J1DGB2 (protein disulfide-isomerase 5-2 OS=Momordica charantia OX=3673 GN=LOC111020237 PE=4 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 8.2e-220
Identity = 382/443 (86.23%), Postives = 418/443 (94.36%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           M RSWQ +VIVLVSIL LPSSSFSS E PLTADG VLELDESNFDLAISSFD+ILVDFYA
Sbjct: 1   MHRSWQVMVIVLVSILGLPSSSFSSVEQPLTADGNVLELDESNFDLAISSFDFILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLAKLK P+VLAK+NADKFTRLAKK+DVDAYPTIKIFMHG+P
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKTPVVLAKVNADKFTRLAKKFDVDAYPTIKIFMHGIP 120

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKA+LLVRYLKKFVAPDVSIL+SDSSIN+FVEA+G YFPIYLGFGLDESIISKF
Sbjct: 121 VDYYGPRKADLLVRYLKKFVAPDVSILDSDSSINDFVEAAGAYFPIYLGFGLDESIISKF 180

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
           AIKYKKKAWFSVAKGFSED+MVSYDFDKVP+LVS+HPNYNNERS FYGPFE++FLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDVMVSYDFDKVPALVSVHPNYNNERSTFYGPFEEQFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFP VLPINYDTLKLLKDD+RKIALTIV+DEDDDQTKNLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPRVLPINYDTLKLLKDDDRKIALTIVEDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEF DSFGDKKTTLPKM+IWDG+++YL VIGSESI+GNDHA++ISKF+EGYREGR
Sbjct: 301 GAKQWGEFTDSFGDKKTTLPKMVIWDGKDDYLTVIGSESILGNDHATQISKFIEGYREGR 360

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TEER++AGPSFIGFINS+IG  +VYI+V IV  +MLFQ LTKDDNEY RVDTP++DQ DQ
Sbjct: 361 TEERKIAGPSFIGFINSLIGIRTVYILVIIVGGIMLFQTLTKDDNEYRRVDTPSRDQFDQ 420

Query: 421 ISG--SSTSQVERTEYRAGDKED 442
            S   S++SQVER EYRAGDK+D
Sbjct: 421 TSSSTSTSSQVERREYRAGDKQD 443

BLAST of CmaCh04G030060 vs. ExPASy TrEMBL
Match: A0A5D3C662 (Protein disulfide-isomerase 5-2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G001260 PE=4 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.8e-219
Identity = 379/441 (85.94%), Postives = 419/441 (95.01%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           M+RSWQ +V+ L+SI ALPS S SSSELPLTADG VL+LDESNFDLAISSFDYILVDFYA
Sbjct: 1   MRRSWQFLVLFLLSISALPSFSLSSSELPLTADGKVLDLDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLA+LKEPIVLAK+NADK+T LAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKAELL RYLKKFVAPDVS+LESDSSINEFVEA+GPYFPIYLGFGLDES+ISKF
Sbjct: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
            IKYKKKAWFSVAKGFSEDIMVSYDFDKVP+L+SIHPNYNNERSIFYGPFE+ FLEEFIK
Sbjct: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALISIHPNYNNERSIFYGPFEEPFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDD+RKIALTIV+DED+DQTK LINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEFADSF DKKTTLPKM+IWDGE++YLMV GSESIIGNDHASEISKF+EGYREGR
Sbjct: 301 GAKQWGEFADSFRDKKTTLPKMVIWDGEDDYLMVTGSESIIGNDHASEISKFIEGYREGR 360

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TE+RRVAGP+ +GFINS+IG  ++YIIV IVA++ML+Q LTK+D+EY+RVDT A+DQV+Q
Sbjct: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAVIMLYQNLTKEDSEYNRVDTSARDQVEQ 420

Query: 421 ISGSSTSQVERTEYRAGDKED 442
            S SS ++V+R+EY+AGDKED
Sbjct: 421 TSSSSAAEVQRSEYKAGDKED 441

BLAST of CmaCh04G030060 vs. ExPASy TrEMBL
Match: A0A1S3B3L3 (protein disulfide-isomerase 5-2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485425 PE=4 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.8e-219
Identity = 379/441 (85.94%), Postives = 419/441 (95.01%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           M+RSWQ +V+ L+SI ALPS S SSSELPLTADG VL+LDESNFDLAISSFDYILVDFYA
Sbjct: 1   MRRSWQFLVLFLLSISALPSFSLSSSELPLTADGKVLDLDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLA+LKEPIVLAK+NADK+T LAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKAELL RYLKKFVAPDVS+LESDSSINEFVEA+GPYFPIYLGFGLDES+ISKF
Sbjct: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
            IKYKKKAWFSVAKGFSEDIMVSYDFDKVP+L+SIHPNYNNERSIFYGPFE+ FLEEFIK
Sbjct: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALISIHPNYNNERSIFYGPFEEPFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDD+RKIALTIV+DED+DQTK LINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEFADSF DKKTTLPKM+IWDGE++YLMV GSESIIGNDHASEISKF+EGYREGR
Sbjct: 301 GAKQWGEFADSFRDKKTTLPKMVIWDGEDDYLMVTGSESIIGNDHASEISKFIEGYREGR 360

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TE+RRVAGP+ +GFINS+IG  ++YIIV IVA++ML+Q LTK+D+EY+RVDT A+DQV+Q
Sbjct: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAVIMLYQNLTKEDSEYNRVDTSARDQVEQ 420

Query: 421 ISGSSTSQVERTEYRAGDKED 442
            S SS ++V+R+EY+AGDKED
Sbjct: 421 TSSSSAAEVQRSEYKAGDKED 441

BLAST of CmaCh04G030060 vs. NCBI nr
Match: XP_022990024.1 (protein disulfide-isomerase 5-2-like [Cucurbita maxima])

HSP 1 Score: 870.2 bits (2247), Expect = 7.8e-249
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
           AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ
Sbjct: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420

Query: 421 ISGSSTSQVERTEYRAGDKED 442
           ISGSSTSQVERTEYRAGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 441

BLAST of CmaCh04G030060 vs. NCBI nr
Match: KAG7033399.1 (Protein disulfide-isomerase 5-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 863.6 bits (2230), Expect = 7.3e-247
Identity = 437/441 (99.09%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           MQRSWQAVVIVLVSILALPSSSFS SELPLTADGTVLELDESNFDLAISSFDYILVDFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSISELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
           AIKYKKKAWFSVAKGFSEDIMVSYDFDKVP+LVSIHPNYNNERSIFYGPFEDKFLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMIIWDG+EEYLMVIGSESIIGNDHASEISKFVEGYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGKEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPA+DQVDQ
Sbjct: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPARDQVDQ 420

Query: 421 ISGSSTSQVERTEYRAGDKED 442
           ISGSSTSQVERTEYRAGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 441

BLAST of CmaCh04G030060 vs. NCBI nr
Match: XP_022958351.1 (protein disulfide-isomerase 5-2-like [Cucurbita moschata])

HSP 1 Score: 858.2 bits (2216), Expect = 3.1e-245
Identity = 433/441 (98.19%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYIL+DFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILIDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
           AIKYKKKAWFSVAKGFSEDIMVSYDFDKVP+LVSIHPNYNNERSIFYGPFEDKFLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMIIWDG+EEYLMVIGSESIIGNDHASEISKFV+GYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGKEEYLMVIGSESIIGNDHASEISKFVQGYREGR 360

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TEERRVAGPSFIGFINSMIGT SVYIIVFIVA++MLFQYLTKDDNEYHRVDTPA+DQVDQ
Sbjct: 361 TEERRVAGPSFIGFINSMIGTKSVYIIVFIVAVIMLFQYLTKDDNEYHRVDTPARDQVDQ 420

Query: 421 ISGSSTSQVERTEYRAGDKED 442
           ISGSSTSQVERTEYRAGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 441

BLAST of CmaCh04G030060 vs. NCBI nr
Match: XP_023533712.1 (protein disulfide-isomerase 5-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 854.0 bits (2205), Expect = 5.8e-244
Identity = 428/441 (97.05%), Postives = 440/441 (99.77%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           MQRSWQ +V+VLVS+L+LPSSSFS+++LPL+ADGTVLELDESNFDLAISSFDYILVDFYA
Sbjct: 11  MQRSWQVLVLVLVSLLSLPSSSFSTADLPLSADGTVLELDESNFDLAISSFDYILVDFYA 70

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP
Sbjct: 71  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 130

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF
Sbjct: 131 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 190

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
           AIKYKKKAWFSVAKGFSEDIMVSYDFDKVP+LVSIHPNYNNERSIFYGPFEDKFLEEFIK
Sbjct: 191 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEDKFLEEFIK 250

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV
Sbjct: 251 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 310

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMIIWDG+EEYLMVIGSESIIGNDHASEIS+FVEGYREGR
Sbjct: 311 GAKQWGEFADSFGDKKTTLPKMIIWDGKEEYLMVIGSESIIGNDHASEISQFVEGYREGR 370

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPA+DQVDQ
Sbjct: 371 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPARDQVDQ 430

Query: 421 ISGSSTSQVERTEYRAGDKED 442
           ISGSSTSQVERTEYRAGDKED
Sbjct: 431 ISGSSTSQVERTEYRAGDKED 451

BLAST of CmaCh04G030060 vs. NCBI nr
Match: KAG6602712.1 (Protein disulfide-isomerase 5-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808.1 bits (2086), Expect = 3.7e-230
Identity = 415/441 (94.10%), Postives = 418/441 (94.78%), Query Frame = 0

Query: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60
           MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180
           VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240
           AIKYKKKAWFSVAKGFSEDIMVSYDFDKVP+LVSIHPNYNNERSIFYGPFEDKFLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMIIWDG+EEYLMVIGSESIIGNDHASEISKFVEGYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGKEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360

Query: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420
           TEERRVA                       VAIVMLFQYLTKDDNEYHRVDTPA+DQVDQ
Sbjct: 361 TEERRVA-----------------------VAIVMLFQYLTKDDNEYHRVDTPARDQVDQ 418

Query: 421 ISGSSTSQVERTEYRAGDKED 442
           ISGSSTSQVERTEYRAGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 418

BLAST of CmaCh04G030060 vs. TAIR 10
Match: AT1G35620.1 (PDI-like 5-2 )

HSP 1 Score: 535.0 bits (1377), Expect = 5.6e-152
Identity = 268/437 (61.33%), Postives = 349/437 (79.86%), Query Frame = 0

Query: 8   VVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYAPWCGHCK 67
           +++  +S L L  S  +SS+   T DGTVLEL +SNFD AIS+FD I VDFYAPWCGHCK
Sbjct: 6   LLLCWISFLTLSISISASSDDQFTLDGTVLELTDSNFDSAISTFDCIFVDFYAPWCGHCK 65

Query: 68  RLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVPVDYYGPR 127
           RL+PELDAAAP LAKLK+PIV+AKLNADK++RLA+K ++DA+PT+ ++ HGVP++YYGPR
Sbjct: 66  RLNPELDAAAPILAKLKQPIVIAKLNADKYSRLARKIEIDAFPTLMLYNHGVPMEYYGPR 125

Query: 128 KAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKFAIKYKKK 187
           KA+LLVRYLKKFVAPDV++LESDS++ EFVE +G +FP+++GFGL+ESIIS    KYKKK
Sbjct: 126 KADLLVRYLKKFVAPDVAVLESDSTVKEFVEDAGTFFPVFIGFGLNESIISGLGRKYKKK 185

Query: 188 AWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLFPLV 247
           AWF+V+K  SED MVSYDFDK P+LV+ HP Y NE S+FYGPFED FLEEF+KQS  PL+
Sbjct: 186 AWFAVSKEVSEDTMVSYDFDKAPALVANHPTY-NEHSVFYGPFEDGFLEEFVKQSFLPLI 245

Query: 248 LPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYVGAKQWGE 307
           LPIN+DTLKLLKDDERKI LTIV+DE  +  + L   L+AAA ANRDLVF YVG KQ+ E
Sbjct: 246 LPINHDTLKLLKDDERKIVLTIVEDETHESLEKLYKALRAAAHANRDLVFGYVGVKQFEE 305

Query: 308 FADSFG-DKKTTLPKMIIWDGEEEYLMVIGSESII-GNDHASEISKFVEGYREGRTEERR 367
           F DSF  DKKT LPK+++WDG+EEY  V G E+I    DH +++S+F+EGYREGRTE+++
Sbjct: 306 FVDSFHVDKKTNLPKIVVWDGDEEYDQVTGIETITQEEDHLTQVSRFLEGYREGRTEKKK 365

Query: 368 VAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTK-DDNEYHRVDTPAQDQVDQISGS 427
           + GPSF+GFINSMIG  SVYI+VF+VA++M+ + L + ++    R  T  +++VDQ   +
Sbjct: 366 INGPSFMGFINSMIGIRSVYILVFLVAVIMMLRSLGQVEEPTGVRTATAVRERVDQ--AT 425

Query: 428 STSQVERTEYRAGDKED 442
           +  + E +E++  DK++
Sbjct: 426 TVPEDESSEHKPSDKKE 439

BLAST of CmaCh04G030060 vs. TAIR 10
Match: AT1G21750.1 (PDI-like 1-1 )

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-28
Identity = 106/374 (28.34%), Postives = 181/374 (48.40%), Query Frame = 0

Query: 13  VSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYAPWCGHCKRLSPE 72
           + +L+L +SS  S E        VL LD +NF   I+  D+I+V+FYAPWCGHCK+L+PE
Sbjct: 11  ILVLSLCASSIRSEE--TETKEFVLTLDHTNFTDTINKHDFIVVEFYAPWCGHCKQLAPE 70

Query: 73  LDAAAPQLAKLKEPIVLAKLNADKFT--RLAKKYDVDAYPTIKIFMHG--VPVDYYGPRK 132
            + AA  L+    P+VLAK++A + T    A +Y+V  +PTIKIF +G     +Y GPR+
Sbjct: 71  YEKAASALSSNVPPVVLAKIDASEETNREFATQYEVQGFPTIKIFRNGGKAVQEYNGPRE 130

Query: 133 AELLVRYLKKFVAPDVSILESDSSINEFVEASG----PYFPIYLGFGLDESIISKFAIKY 192
           AE +V YLKK   P  + ++S    +E V          FP   G   D    S  AI  
Sbjct: 131 AEGIVTYLKKQSGPASAEIKSADDASEVVSDKKVVVVGIFPKLSGSEFD----SFMAIAE 190

Query: 193 KKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLF 252
           K ++    A      ++   +      +V +   + +E+ +    F+ + LE+F+K+S  
Sbjct: 191 KLRSELDFAHTSDAKLLPRGESSVTGPVVRLFKPF-DEQFVDSKDFDGEALEKFVKESSI 250

Query: 253 PLVLPINYD------TLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANR--DLV 312
           PL+   + D       +K  +    K  L I  +   +  ++L +  +  A++N+   L 
Sbjct: 251 PLITVFDKDPNNHPYVIKFFESTNTKAMLFI--NFTGEGAESLKSKYREVATSNKGQGLS 310

Query: 313 FAYVGAKQWGEFADSFGDKKTTLPKMIIWDGEE-EYLMVIGSESIIGNDHASEISKFVEG 370
           F    A+        FG +++ +P +II   ++ +YL          N    +I  +V+ 
Sbjct: 311 FLLGDAENSQGAFQYFGLEESQVPLIIIQTADDKKYLKT--------NVEVDQIESWVKD 367

BLAST of CmaCh04G030060 vs. TAIR 10
Match: AT1G21750.2 (PDI-like 1-1 )

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-28
Identity = 106/374 (28.34%), Postives = 181/374 (48.40%), Query Frame = 0

Query: 13  VSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYAPWCGHCKRLSPE 72
           + +L+L +SS  S E        VL LD +NF   I+  D+I+V+FYAPWCGHCK+L+PE
Sbjct: 11  ILVLSLCASSIRSEE--TETKEFVLTLDHTNFTDTINKHDFIVVEFYAPWCGHCKQLAPE 70

Query: 73  LDAAAPQLAKLKEPIVLAKLNADKFT--RLAKKYDVDAYPTIKIFMHG--VPVDYYGPRK 132
            + AA  L+    P+VLAK++A + T    A +Y+V  +PTIKIF +G     +Y GPR+
Sbjct: 71  YEKAASALSSNVPPVVLAKIDASEETNREFATQYEVQGFPTIKIFRNGGKAVQEYNGPRE 130

Query: 133 AELLVRYLKKFVAPDVSILESDSSINEFVEASG----PYFPIYLGFGLDESIISKFAIKY 192
           AE +V YLKK   P  + ++S    +E V          FP   G   D    S  AI  
Sbjct: 131 AEGIVTYLKKQSGPASAEIKSADDASEVVSDKKVVVVGIFPKLSGSEFD----SFMAIAE 190

Query: 193 KKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLF 252
           K ++    A      ++   +      +V +   + +E+ +    F+ + LE+F+K+S  
Sbjct: 191 KLRSELDFAHTSDAKLLPRGESSVTGPVVRLFKPF-DEQFVDSKDFDGEALEKFVKESSI 250

Query: 253 PLVLPINYD------TLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANR--DLV 312
           PL+   + D       +K  +    K  L I  +   +  ++L +  +  A++N+   L 
Sbjct: 251 PLITVFDKDPNNHPYVIKFFESTNTKAMLFI--NFTGEGAESLKSKYREVATSNKGQGLS 310

Query: 313 FAYVGAKQWGEFADSFGDKKTTLPKMIIWDGEE-EYLMVIGSESIIGNDHASEISKFVEG 370
           F    A+        FG +++ +P +II   ++ +YL          N    +I  +V+ 
Sbjct: 311 FLLGDAENSQGAFQYFGLEESQVPLIIIQTADDKKYLKT--------NVEVDQIESWVKD 367

BLAST of CmaCh04G030060 vs. TAIR 10
Match: AT1G77510.1 (PDI-like 1-2 )

HSP 1 Score: 118.6 bits (296), Expect = 1.2e-26
Identity = 108/369 (29.27%), Postives = 179/369 (48.51%), Query Frame = 0

Query: 14  SILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYAPWCGHCKRLSPEL 73
           SIL L S   SS     T +  VL LD SNF   IS  D+I+V+FYAPWCGHC++L+PE 
Sbjct: 10  SILLLLSLFVSSIRSEETKE-FVLTLDHSNFTETISKHDFIVVEFYAPWCGHCQKLAPEY 69

Query: 74  DAAAPQLAKLKEPIVLAKLNADKFT--RLAKKYDVDAYPTIKIFMHG--VPVDYYGPRKA 133
           + AA +L+    P+ LAK++A +      A +Y +  +PT+KI  +G     DY GPR+A
Sbjct: 70  EKAASELSSHNPPLALAKIDASEEANKEFANEYKIQGFPTLKILRNGGKSVQDYNGPREA 129

Query: 134 ELLVRYLKKFVAP-DVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKFAIKYKKKA 193
           E +V YLKK   P  V I  +DS+     E +     ++     DE   S  A+  K +A
Sbjct: 130 EGIVTYLKKQSGPASVEIKSADSATEVVGEKNVVAVGVFPKLSGDE-FDSFMALAEKLRA 189

Query: 194 WFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLFPLVL 253
            +  A       +   +  + P++    P   +E  +    F  + LE+F+K+S  PLV 
Sbjct: 190 DYDFAHTLDAKFLPRGESVEGPAVRLFKP--FDELFVDSKDFNGEALEKFVKESSIPLVT 249

Query: 254 -----PINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAY-VGA 313
                P N+  +    +     A+  V +      + L +  +  A++N+D   A+ VG 
Sbjct: 250 VFDSDPNNHPYVAKFFESPATKAMMFV-NFTGATAEALKSKYREVATSNKDQSLAFLVGD 309

Query: 314 KQWGEFA-DSFGDKKTTLPKMIIWDGE-EEYLMVIGSESIIGNDHASEISKFVEGYREGR 370
            +  + A   FG +++ +P +II   + ++YL V        N    +I  + + +++G+
Sbjct: 310 AESSQGAFQYFGLEESQVPLIIIQTPDNKKYLKV--------NVEVDQIESWFKDFQDGK 365

BLAST of CmaCh04G030060 vs. TAIR 10
Match: AT3G54960.1 (PDI-like 1-3 )

HSP 1 Score: 105.5 bits (262), Expect = 1.1e-22
Identity = 84/318 (26.42%), Postives = 147/318 (46.23%), Query Frame = 0

Query: 23  FSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYAPWCGHCKRLSPELDAAAPQLAK 82
           +    LP   +  V  L + NF   + +  + +V+FYAPWCG C+ L+PE  AAA +L  
Sbjct: 88  YEEEPLPPVDEKDVAVLTKDNFTEFVGNNSFAMVEFYAPWCGACQALTPEYAAAATELKG 147

Query: 83  LKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHG-VPVDYYGPRKAELLVRYLKKFVA 142
           L     LAK++A +   LA+KY++  +PT+ +F+ G +   Y G R  + +V +LKK  +
Sbjct: 148 L---AALAKIDATEEGDLAQKYEIQGFPTVFLFVDGEMRKTYEGERTKDGIVTWLKKKAS 207

Query: 143 PDVSILESDSSINEFVEASGPYFPIYLGFGLDESII----SKFAIKYKKKAWFSVAKGFS 202
           P +  + +       + A        L FG   S++     + A   + +   S  +  S
Sbjct: 208 PSIHNITTKEEAERVLSAEPK-----LVFGFLNSLVGSESEELAAASRLEDDLSFYQTAS 267

Query: 203 EDIMVSYDFD---KVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIKQSLFPLVLPINYDT 262
            DI   ++ +   K P+LV +      + + F G F    + EF+  +  PLV+    + 
Sbjct: 268 PDIAKLFEIETQVKRPALVLLKKE-EEKLARFDGNFTKTAIAEFVSANKVPLVINFTREG 327

Query: 263 LKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV--GAKQWGEFADSF 322
             L+ +   K  L +    ++ + K+L  L + A S     VF YV    + +GE    F
Sbjct: 328 ASLIFESSVKNQLILFAKANESE-KHLPTLREVAKSFKGKFVFVYVQMDNEDYGEAVSGF 387

Query: 323 GDKKTTLPKMIIWDGEEE 331
                  PK++++ G E+
Sbjct: 388 FGVTGAAPKVLVYTGNED 395

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94F097.8e-15161.33Protein disulfide-isomerase 5-2 OS=Arabidopsis thaliana OX=3702 GN=PDIL5-2 PE=1 ... [more]
Q0JD421.8e-13156.06Protein disulfide isomerase-like 5-2 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q0E0I18.5e-12153.16Protein disulfide isomerase-like 5-3 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P053078.4e-2831.85Protein disulfide-isomerase OS=Bos taurus OX=9913 GN=P4HB PE=1 SV=1[more]
P543991.1e-2727.03Protein disulfide-isomerase OS=Drosophila melanogaster OX=7227 GN=Pdi PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JQY33.8e-249100.00protein disulfide-isomerase 5-2-like OS=Cucurbita maxima OX=3661 GN=LOC111487041... [more]
A0A6J1H1U71.5e-24598.19protein disulfide-isomerase 5-2-like OS=Cucurbita moschata OX=3662 GN=LOC1114595... [more]
A0A6J1DGB28.2e-22086.23protein disulfide-isomerase 5-2 OS=Momordica charantia OX=3673 GN=LOC111020237 P... [more]
A0A5D3C6621.8e-21985.94Protein disulfide-isomerase 5-2 isoform X1 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B3L31.8e-21985.94protein disulfide-isomerase 5-2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034854... [more]
Match NameE-valueIdentityDescription
XP_022990024.17.8e-249100.00protein disulfide-isomerase 5-2-like [Cucurbita maxima][more]
KAG7033399.17.3e-24799.09Protein disulfide-isomerase 5-2, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022958351.13.1e-24598.19protein disulfide-isomerase 5-2-like [Cucurbita moschata][more]
XP_023533712.15.8e-24497.05protein disulfide-isomerase 5-2-like [Cucurbita pepo subsp. pepo][more]
KAG6602712.13.7e-23094.10Protein disulfide-isomerase 5-2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G35620.15.6e-15261.33PDI-like 5-2 [more]
AT1G21750.11.3e-2828.34PDI-like 1-1 [more]
AT1G21750.21.3e-2828.34PDI-like 1-1 [more]
AT1G77510.11.2e-2629.27PDI-like 1-2 [more]
AT3G54960.11.1e-2226.42PDI-like 1-3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00421THIOREDOXINcoord: 54..62
score: 63.02
coord: 105..116
score: 37.27
coord: 62..71
score: 60.33
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 23..151
e-value: 2.0E-34
score: 120.2
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 232..365
e-value: 1.9E-13
score: 52.9
NoneNo IPR availablePFAMPF13848Thioredoxin_6coord: 178..336
e-value: 5.6E-13
score: 49.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 411..441
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 415..431
NoneNo IPR availablePANTHERPTHR18929:SF218PROTEIN DISULFIDE-ISOMERASE 5-2coord: 9..441
NoneNo IPR availablePANTHERPTHR18929PROTEIN DISULFIDE ISOMERASEcoord: 9..441
NoneNo IPR availableCDDcd02961PDI_a_familycoord: 37..136
e-value: 6.76816E-32
score: 115.4
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 36..137
e-value: 8.8E-25
score: 86.7
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 17..141
score: 14.897723
IPR017937Thioredoxin, conserved sitePROSITEPS00194THIOREDOXIN_1coord: 55..73
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 28..139
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 243..362

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G030060.1CmaCh04G030060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0034976 response to endoplasmic reticulum stress
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003756 protein disulfide isomerase activity