CmaCh04G030000 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G030000
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	alkylated DNA repair protein alkB homolog 8-like
Location	Cma_Chr04: 19622601 .. 19625011 (+)
RNA-Seq Expression	CmaCh04G030000
Synteny	CmaCh04G030000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTCTATTTGTCTTTGTGAGTATTTTCCACAAGTAGTAGTTGACTAGACCTGTCTAAATGAGATTGGTTTTCCTCCTGGACAAGGCTAAACTATGAAGCAAGAAGAATCAGACCTTTGATTTTAGAGGGAACTGTTTGGGGAAATTGTTAAGGAACTGAGAAGAGCTCCAATCTTTGCCGTGTTCTAGCAGTAAATGAATGAATGAAAAATATCCTCTTGGCTATCATATTTTCTAAAGAACTTAGAATTCAAAATGACCTTCACATTTTTTTATCCTACTCCTTGAATGTAGGAATCTCTTGACGCTATCATGTTTCGTGTATGTCTTTGTGTACGAGTGTTTGTATAATAGACTTCTATACAGTGAAAGAACTGCAAGAAATTTAGTCCTTGGAGAGCCATGGTTTCTTGCAGGGTATGCCTAATTGATTTTCTTGGCTCAAGCTTTTGGCTGTGCTACAGGAGTGAGCAAAGAGATTAAAGTCTTTGGTTTATCCTCTGTTGTAAGTGGTAGTGGGCATCATTCTAGACTTTCCAGTGCTATGAAAGAGATTAAAGTCAGAAGTGGTTCTAATGCAGATCCTGATCTGTTGAATGATGAAACACATGCACAGCAATTGACTTCTGCAAATCAGAGCTGTTCATCATCAAATGTGCAGTGCACCCCTGAAATTGAAAAGAAATATGTGCATCGTGTTTATGATGCCATTGCTCCCCATTTTAGTTCAACTCGGTTTGCAAAGTGGCCAAAAGTTGCATCCTTTTTATCATCGTTACCTCTAGGGTCCCTCGTCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTAATCCCAACTGCTTTTTCATTGGATGTGATATTAGTGCCCAACTTATCAAAATTTGTGATGAGAGGGGACATGAAGTTTTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCGATCTCTATAGCTGTATTACATCATCTAAGTACTGAAAACAGGAGGAGGAAAGCAATCGAAGAATTAATACGTGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGATAAATCTTTATTGACGAAATGGATGCCACTTTCAGAGAAGTATGTTGATGAGTGGGTTGGACCAGGTAGTCCACGTGTTCGTAGTCCATCGTCGTTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATCATTCAGGTGTTTCCCTAAAGGATTCCAAGGAAAACTTCACTGGAAGCAAGCCAGAAAATAAACCTCTACCCTCTCGGAGTGGAAATGATTTTATGAATTGTAATGATGAAAACTTATTGAAGATCCAGCAAGAATATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGGTCGTCTGCCAGTGCCCTTGCTAGTGGTTTGGCAAAGAAGGATGATAAGAAAGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGTATTCCTCTCTCTCATTTCTCTTGTATGGTTTCTTCTTTCTTGGCTTCTTTTACTTATTGGATATGGGGGGGAGGCTGTTCTTTAGTTGTGACTAGGCTGTTCTAGATCTATGTGTTGAAGTGTGTCTCTATTAGCAGTAACCTTTCAACTACTTCTAGTTCCATTAATGTGTGATAATGTGAAAAGTGATGACCATGGTCGTTCCCGAGCTTGCAACATTTCATCTTTTGACTCGAGATACCATGACTATGTAGAAATAGGGAACACGGTATATATCATGCACTTCACGACAACATTTCTACATACATCTTTCTTGGCCTTTTGACTAAGATTAAGAGATATCTACATTTCTACATAATGATTTGTTCTGTTACTGCTTGATCATCTTGGGTGCATTTGTAGGTTGGTGTCTGGTATGGACGACGCGGTAGTGGTCGATCGATTTTATGACAAATCAAACTGGTGTGTAGTTCTTGAGAAAACAGTGTGAGGAATGAAATGAGGTATTATGAAGATGATTCAATCTTGCCTGCTTGTTGTGCCCACTCAAACCGTGCTGAAGGTGGTCTATGGAGTTGTATCACTGTTGCTATCTCGGCTTTGTTTTTGTCGTCCAGTCATAGTTTGGGGGTAGCAATCTCTGCAGGCTCTTTAGCCAAAAAAAATGCTGTCACTGTTAAGGATTTACGATTGTGTATTATGATTAATAAGGCGGTGGATTGCCCTTTGATTCAGTTCTAAATTGTTTGTGTTGAAAAGGTTGGGCTTCTATCTGCTATCCCTTGAATAAATCATTGATATTATACGGTTTTAGGCTCAGGTTCTCGATCAAGCATTTATTGATTTAGAAAACTTGCCGACTGTAATTTGTTAAATGAGGTTGGACAGCCG

mRNA sequence

ATGTTTTCTATTTGTCTTTGAGTGAGCAAAGAGATTAAAGTCTTTGGTTTATCCTCTGTTGTAAGTGGTAGTGGGCATCATTCTAGACTTTCCAGTGCTATGAAAGAGATTAAAGTCAGAAGTGGTTCTAATGCAGATCCTGATCTGTTGAATGATGAAACACATGCACAGCAATTGACTTCTGCAAATCAGAGCTGTTCATCATCAAATGTGCAGTGCACCCCTGAAATTGAAAAGAAATATGTGCATCGTGTTTATGATGCCATTGCTCCCCATTTTAGTTCAACTCGGTTTGCAAAGTGGCCAAAAGTTGCATCCTTTTTATCATCGTTACCTCTAGGGTCCCTCGTCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTAATCCCAACTGCTTTTTCATTGGATGTGATATTAGTGCCCAACTTATCAAAATTTGTGATGAGAGGGGACATGAAGTTTTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCGATCTCTATAGCTGTATTACATCATCTAAGTACTGAAAACAGGAGGAGGAAAGCAATCGAAGAATTAATACGTGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGATAAATCTTTATTGACGAAATGGATGCCACTTTCAGAGAAGTATGTTGATGAGTGGGTTGGACCAGGTAGTCCACGTGTTCGTAGTCCATCGTCGTTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATCATTCAGGTGTTTCCCTAAAGGATTCCAAGGAAAACTTCACTGGAAGCAAGCCAGAAAATAAACCTCTACCCTCTCGGAGTGGAAATGATTTTATGAATTGTAATGATGAAAACTTATTGAAGATCCAGCAAGAATATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGGTCGTCTGCCAGTGCCCTTGCTAGTGGTTTGGCAAAGAAGGATGATAAGAAAGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGTTGGTGTCTGGTATGGACGACGCGGTAGTGGTCGATCGATTTTATGACAAATCAAACTGGTGTGTAGTTCTTGAGAAAACAGTGTGAGGAATGAAATGAGGTATTATGAAGATGATTCAATCTTGCCTGCTTGTTGTGCCCACTCAAACCGTGCTGAAGGTGGTCTATGGAGTTGTATCACTGTTGCTATCTCGGCTTTGTTTTTGTCGTCCAGTCATAGTTTGGGGGTAGCAATCTCTGCAGGCTCTTTAGCCAAAAAAAATGCTGTCACTGTTAAGGATTTACGATTGTGTATTATGATTAATAAGGCGGTGGATTGCCCTTTGATTCAGTTCTAAATTGTTTGTGTTGAAAAGGTTGGGCTTCTATCTGCTATCCCTTGAATAAATCATTGATATTATACGGTTTTAGGCTCAGGTTCTCGATCAAGCATTTATTGATTTAGAAAACTTGCCGACTGTAATTTGTTAAATGAGGTTGGACAGCCG

Coding sequence (CDS)

ATGAAAGAGATTAAAGTCAGAAGTGGTTCTAATGCAGATCCTGATCTGTTGAATGATGAAACACATGCACAGCAATTGACTTCTGCAAATCAGAGCTGTTCATCATCAAATGTGCAGTGCACCCCTGAAATTGAAAAGAAATATGTGCATCGTGTTTATGATGCCATTGCTCCCCATTTTAGTTCAACTCGGTTTGCAAAGTGGCCAAAAGTTGCATCCTTTTTATCATCGTTACCTCTAGGGTCCCTCGTCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTAATCCCAACTGCTTTTTCATTGGATGTGATATTAGTGCCCAACTTATCAAAATTTGTGATGAGAGGGGACATGAAGTTTTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCGATCTCTATAGCTGTATTACATCATCTAAGTACTGAAAACAGGAGGAGGAAAGCAATCGAAGAATTAATACGTGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGATAAATCTTTATTGACGAAATGGATGCCACTTTCAGAGAAGTATGTTGATGAGTGGGTTGGACCAGGTAGTCCACGTGTTCGTAGTCCATCGTCGTTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATCATTCAGGTGTTTCCCTAAAGGATTCCAAGGAAAACTTCACTGGAAGCAAGCCAGAAAATAAACCTCTACCCTCTCGGAGTGGAAATGATTTTATGAATTGTAATGATGAAAACTTATTGAAGATCCAGCAAGAATATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGGTCGTCTGCCAGTGCCCTTGCTAGTGGTTTGGCAAAGAAGGATGATAAGAAAGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGTTGGTGTCTGGTATGGACGACGCGGTAGTGGTCGATCGATTTTATGACAAATCAAACTGGTGTGTAGTTCTTGAGAAAACAGTGTGA

Protein sequence

MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENFTGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV

Homology

[Close]

BLAST of CmaCh04G030000 vs. ExPASy Swiss-Prot
Match: Q94A09 (tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=TRM9 PE=1 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.4e-129
Identity = 247/373 (66.22%), Postives = 284/373 (76.14%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSS--SNVQCTPEIEKKYVHRVYDAIAP 60
           M++IKV+S S    + L      ++  S   S SS  S+V+ TPEIEKKYVHRVYDAIAP
Sbjct: 29  MRDIKVKSDSK---EFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHRVYDAIAP 88

Query: 61  HFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDE 120
           HFSSTRFAKWPKVA+FL SLP GS++LDAGCGNGKYLG NP+CFFIGCDIS  LIKIC +
Sbjct: 89  HFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHPLIKICSD 148

Query: 121 RGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVW 180
           +G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRR+KAIEEL+RVVK GG VLITVW
Sbjct: 149 KGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGGFVLITVW 208

Query: 181 AVEQEDKSLLTKWMPLSEKYVDEWVGPG----SPRVRSPSSLALESIPEM---------- 240
           A EQED SLLTKW PLS KYV+EWVGPG    SPRVR+    +LESIPE           
Sbjct: 209 AAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVSTKEQKVE 268

Query: 241 NENHSGV-SLKDSKENFTGSK-----PENK-PLPSRSGNDFMNCNDENLLKIQQEYFVPW 300
           N    G+ S+ +S+E+    K     PE K  +  +     +  + E L K QQEYFVPW
Sbjct: 269 NSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQQEYFVPW 328

Query: 301 HLPYHRAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDR 351
           HLPYHRAEVSG+SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ +A++VDR
Sbjct: 329 HLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVGNAMIVDR 388

[Close]

BLAST of CmaCh04G030000 vs. ExPASy Swiss-Prot
Match: Q80Y20 (Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 4.0e-57
Identity = 124/312 (39.74%), Postives = 175/312 (56.09%), Query Frame = 0


Query: 43  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCF 102
           E+E+K+VH+VY+ IA HFSSTR + WP++  FL +LP GS+V D GCGNGKYLG N + +
Sbjct: 369 ELEQKHVHQVYNEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKDLY 428

Query: 103 FIGCDISAQLIKICDERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEE 162
            IGCD S  L+ IC ER  + LV DA+ +P R+G  DA ISIAV+HH +T  RR +A++E
Sbjct: 429 MIGCDRSQNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAERRVEALQE 488

Query: 163 LIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIP 222
           L R+++ GG  LI VWA+EQE K+  +K+  L  K + +          S     +   P
Sbjct: 489 LARLLRPGGQALIYVWAMEQEYKNQKSKY--LRGKRISQGDKDELNSATSTEEFLVNQTP 548

Query: 223 EMNENHSGVSLKDS----KENFTGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWH 282
           E       +S+  S    +E +   K  N  LP        +          Q+  VPWH
Sbjct: 549 EGVNEDPALSVNSSSITKEEEYKSRKVPNSELPIHINRTCFH---------SQDVLVPWH 608

Query: 283 LPYHRAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRF 342
           L  +     G   +   SG+A   D   + V++RYYHVF +GELE     + D  ++  +
Sbjct: 609 LKRN----PGKDKAIEPSGVAGCPD--PSPVFHRYYHVFCDGELEASCQAVGDVSILQSY 663

Query: 343 YDKSNWCVVLEK 351
           YD+ NWCVVL+K
Sbjct: 669 YDQGNWCVVLQK 663

[Close]

BLAST of CmaCh04G030000 vs. ExPASy Swiss-Prot
Match: Q07G10 (Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alkbh8 PE=2 SV=2)

HSP 1 Score: 221.5 bits (563), Expect = 1.5e-56
Identity = 128/308 (41.56%), Postives = 172/308 (55.84%), Query Frame = 0


Query: 44  IEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFF 103
           +E++YVH+VYD IA HFSSTR   WPK+  FL+SLP GSLV D GCGNGKYLG N +   
Sbjct: 369 LEREYVHKVYDDIAGHFSSTRHTPWPKIGDFLASLPKGSLVADVGCGNGKYLGVNKDLCM 428

Query: 104 IGCDISAQLIKICDERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEEL 163
           IGCD S  L+ IC ER  E  V DA+++P+R G  DA ISIAV+HH +TE RR  A++EL
Sbjct: 429 IGCDRSKNLVDICRERTFEAFVCDALSVPFRAGAFDACISIAVIHHFATEERRIAALQEL 488

Query: 164 IRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPE 223
           IR+++KGG  LI VWA+EQE K           KY+ E     S   + PSS AL     
Sbjct: 489 IRLLRKGGKALIYVWALEQEYKK-------NKSKYLKE-----SKTSQGPSSDAL----- 548

Query: 224 MNENHSGVSLKDSKENFTGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHL-PYH 283
                 G +L               PLP  +     +          Q+  VPWHL P +
Sbjct: 549 ------GTNL-----------AVKAPLPIHTNRTSFH---------SQDLLVPWHLKPTN 608

Query: 284 RAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKS 343
           +++V+  +          + +     VY+R+YHVF EGELE + + + +  V   ++D+ 
Sbjct: 609 KSKVTPENKE------QNEKEHGPDSVYHRFYHVFCEGELEAMCNRLSNVAVQHSYHDQG 627

Query: 344 NWCVVLEK 351
           NWCV+LEK
Sbjct: 669 NWCVILEK 627

[Close]

BLAST of CmaCh04G030000 vs. ExPASy Swiss-Prot
Match: A1A4L5 (Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.1e-54
Identity = 122/317 (38.49%), Postives = 171/317 (53.94%), Query Frame = 0


Query: 43  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCF 102
           ++E+ YVHRVY+ IA HFSSTR   WP +  FL +LP GSLV D GCGNGKYLG N   +
Sbjct: 369 QLEQDYVHRVYEEIARHFSSTRHTPWPHIVEFLKALPSGSLVADIGCGNGKYLGINNELY 428

Query: 103 FIGCDISAQLIKICDERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEE 162
            IGCD S  L+ IC ER ++  V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E
Sbjct: 429 MIGCDHSQNLVDICRERQYQAFVCDALAVPIRSGSCDACISIAVIHHFATAERRVAALQE 488

Query: 163 LIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIP 222
           L+R+++ GG  LI VWA+EQE     +K+  L E    + +        S   L ++ +P
Sbjct: 489 LVRLLRPGGKALIYVWAMEQEYNKKKSKY--LRENRTSQGMKEEISNDASVQELLVKQLP 548

Query: 223 EMNEN---HSGVSLKDSKENFTGSK-PENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWH 282
            +      HS  S+ D ++    SK   N  LP  +     +          Q+  VPWH
Sbjct: 549 NVGNQDSAHSVSSINDFQDGGCNSKNVANSKLPIHTNRTSFH---------SQDLLVPWH 608

Query: 283 LPYH-----RAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDDAV 342
              +     R E  G   S           +    V++RYYHVF EGELE     +++  
Sbjct: 609 FKGNPGKDKRVEPFGPLGS-----------RDPGPVFHRYYHVFCEGELEAACRTLNNIS 663

Query: 343 VVDRFYDKSNWCVVLEK 351
           ++  +YD+ NWCV+L+K
Sbjct: 669 ILQSYYDQGNWCVILQK 663

[Close]

BLAST of CmaCh04G030000 vs. ExPASy Swiss-Prot
Match: Q95K79 (Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.6e-53
Identity = 118/311 (37.94%), Postives = 169/311 (54.34%), Query Frame = 0


Query: 44  IEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFF 103
           +E++YVH+VY+ IA HFSSTR   WP +  FL +LP GS+V D GCGNGKYLG N   + 
Sbjct: 370 LEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKYLGVNKELYM 429

Query: 104 IGCDISAQLIKICDERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEEL 163
           +GCD S  L+ IC ER  +  V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E+
Sbjct: 430 VGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAERRVAALQEI 489

Query: 164 IRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPE 223
           +R+++ GG  LI VWA+EQE     +K++  +     +     S    S     +E +P+
Sbjct: 490 VRLLRPGGKALIYVWAMEQEYNKQKSKYLKGNRNSQGKKEEMNSD--TSVQRSLVEQMPD 549

Query: 224 MNENHSGVS---LKDSKENFTGSKP-ENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHL 283
           M    S  S   + DS+E    S+   N  LP       ++ N  +     Q+  VPWHL
Sbjct: 550 MGSRDSASSVPRINDSQEGGCNSRQVSNSKLP-------IHVNRTSF--YSQDMLVPWHL 609

Query: 284 PYHRAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFY 343
                                   +  + V++RYYHVF EGELE L   + D  ++  +Y
Sbjct: 610 K------GNPDKGKPVEPFGPIGSQDPSPVFHRYYHVFREGELEALCRTVSDVRILQSYY 663

Query: 344 DKSNWCVVLEK 351
           D+ NWCV+L+K
Sbjct: 670 DQGNWCVILQK 663

[Close]

BLAST of CmaCh04G030000 vs. ExPASy TrEMBL
Match: A0A6J1JQT2 (alkylated DNA repair protein alkB homolog 8-like OS=Cucurbita maxima OX=3661 GN=LOC111487507 PE=4 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.1e-202
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG
Sbjct: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV
Sbjct: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF
Sbjct: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK
Sbjct: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV
Sbjct: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 352

[Close]

BLAST of CmaCh04G030000 vs. ExPASy TrEMBL
Match: A0A6J1HLF5 (alkylated DNA repair protein alkB homolog 8-like OS=Cucurbita moschata OX=3662 GN=LOC111464581 PE=4 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 1.1e-201
Identity = 350/352 (99.43%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKVRSGSNADPDLLNDETHAQQLTS+NQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSSNQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG
Sbjct: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV
Sbjct: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF
Sbjct: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK
Sbjct: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKK+AVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV
Sbjct: 301 KDDKKSAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 352

[Close]

BLAST of CmaCh04G030000 vs. ExPASy TrEMBL
Match: A0A6J1JY21 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489322 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.6e-184
Identity = 319/352 (90.62%), Postives = 333/352 (94.60%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKV+ GSNADP LLNDE HA QL+ ANQ CS+SNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 41  MKEIKVKGGSNADPHLLNDEAHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHF 100

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLP GSL+LDAGCGNGKYLGFN NCFFIGCDIS QLIKIC+ERG
Sbjct: 101 SSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERG 160

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRR+KAIEEL+RVVKKGGLVLITVWAV
Sbjct: 161 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAV 220

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYV+EWVGPGSPRVRSPSSLALESIPEMNEN+SG  LKDSK NF
Sbjct: 221 EQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNF 280

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGS PEN PLPS+ GNDF NCNDENL K QQEYFVPWHLPYHRAEVSG+SA ALASGLAK
Sbjct: 281 TGSMPENIPLPSQGGNDFANCNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAK 340

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKKAAVVYNRYYHVFSEGELERLVSGMD+AVVVDRFYDKSNWC+VLEKT+
Sbjct: 341 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

[Close]

BLAST of CmaCh04G030000 vs. ExPASy TrEMBL
Match: A0A6J1K1M9 (alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489322 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.6e-184
Identity = 319/352 (90.62%), Postives = 333/352 (94.60%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKV+ GSNADP LLNDE HA QL+ ANQ CS+SNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 94  MKEIKVKGGSNADPHLLNDEAHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHF 153

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLP GSL+LDAGCGNGKYLGFN NCFFIGCDIS QLIKIC+ERG
Sbjct: 154 SSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERG 213

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRR+KAIEEL+RVVKKGGLVLITVWAV
Sbjct: 214 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAV 273

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYV+EWVGPGSPRVRSPSSLALESIPEMNEN+SG  LKDSK NF
Sbjct: 274 EQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNF 333

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGS PEN PLPS+ GNDF NCNDENL K QQEYFVPWHLPYHRAEVSG+SA ALASGLAK
Sbjct: 334 TGSMPENIPLPSQGGNDFANCNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAK 393

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKKAAVVYNRYYHVFSEGELERLVSGMD+AVVVDRFYDKSNWC+VLEKT+
Sbjct: 394 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 445

[Close]

BLAST of CmaCh04G030000 vs. ExPASy TrEMBL
Match: A0A6J1FLV5 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445406 PE=4 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 2.9e-183
Identity = 318/352 (90.34%), Postives = 332/352 (94.32%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKV+ GSNADP LLNDE HA QL+ ANQ CS+SNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 41  MKEIKVKGGSNADPHLLNDEAHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHF 100

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLP GSL+LDAGCGNGKYLGFN NCFFIGCDIS QLIKIC+ERG
Sbjct: 101 SSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERG 160

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRR+KAIEEL+RVVKKGGLVLITVWAV
Sbjct: 161 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAV 220

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYV+EWVGPGSPRVRSPSSLALESIPEMNEN+SG  LKDSK NF
Sbjct: 221 EQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNF 280

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGS P N PLPS+SGND  NCNDENL K QQEYFVPWHLPYHRAEVSG+SA ALASGLAK
Sbjct: 281 TGSMPGNIPLPSQSGNDLANCNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAK 340

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKKAAVVYNRYYHVFSEGELERLVSGMD+AVVVDRFYDKSNWC+VLEKT+
Sbjct: 341 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

[Close]

BLAST of CmaCh04G030000 vs. NCBI nr
Match: XP_022990695.1 (alkylated DNA repair protein alkB homolog 8-like [Cucurbita maxima] >XP_022990696.1 alkylated DNA repair protein alkB homolog 8-like [Cucurbita maxima] >XP_022990697.1 alkylated DNA repair protein alkB homolog 8-like [Cucurbita maxima])

HSP 1 Score: 714.5 bits (1843), Expect = 4.4e-202
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG
Sbjct: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV
Sbjct: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF
Sbjct: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK
Sbjct: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV
Sbjct: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 352

[Close]

BLAST of CmaCh04G030000 vs. NCBI nr
Match: XP_022964628.1 (alkylated DNA repair protein alkB homolog 8-like [Cucurbita moschata] >XP_022964634.1 alkylated DNA repair protein alkB homolog 8-like [Cucurbita moschata] >XP_022964635.1 alkylated DNA repair protein alkB homolog 8-like [Cucurbita moschata])

HSP 1 Score: 712.2 bits (1837), Expect = 2.2e-201
Identity = 350/352 (99.43%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKVRSGSNADPDLLNDETHAQQLTS+NQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSSNQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG
Sbjct: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV
Sbjct: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF
Sbjct: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK
Sbjct: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKK+AVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV
Sbjct: 301 KDDKKSAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 352

[Close]

BLAST of CmaCh04G030000 vs. NCBI nr
Match: KAG6602706.1 (tRNA (carboxymethyluridine(34)-5-O)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 709.9 bits (1831), Expect = 1.1e-200
Identity = 350/352 (99.43%), Postives = 351/352 (99.72%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG
Sbjct: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV
Sbjct: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSK +F
Sbjct: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKGSF 240

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK
Sbjct: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV
Sbjct: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 352

[Close]

BLAST of CmaCh04G030000 vs. NCBI nr
Match: XP_023518340.1 (alkylated DNA repair protein alkB homolog 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 695.3 bits (1793), Expect = 2.8e-196
Identity = 341/352 (96.88%), Postives = 345/352 (98.01%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKV+ GSNADP LLNDE HA QL+ A Q CS+SNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 41  MKEIKVKGGSNADPHLLNDEAHAPQLSCATQGCSASNVQCTPEIEKKYVHRVYDAIAPHF 100

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG
Sbjct: 101 SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 160

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV
Sbjct: 161 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 220

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENH+GVSLKDSKENF
Sbjct: 221 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHAGVSLKDSKENF 280

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK
Sbjct: 281 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 340

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV
Sbjct: 341 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 392

[Close]

BLAST of CmaCh04G030000 vs. NCBI nr
Match: XP_022993245.1 (alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 654.4 bits (1687), Expect = 5.4e-184
Identity = 319/352 (90.62%), Postives = 333/352 (94.60%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSSSNVQCTPEIEKKYVHRVYDAIAPHF 60
           MKEIKV+ GSNADP LLNDE HA QL+ ANQ CS+SNVQCTPEIEKKYVHRVYDAIAPHF
Sbjct: 41  MKEIKVKGGSNADPHLLNDEAHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHF 100

Query: 61  SSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERG 120
           SSTRFAKWPKVASFLSSLP GSL+LDAGCGNGKYLGFN NCFFIGCDIS QLIKIC+ERG
Sbjct: 101 SSTRFAKWPKVASFLSSLPRGSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERG 160

Query: 121 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAV 180
           HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRR+KAIEEL+RVVKKGGLVLITVWAV
Sbjct: 161 HEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAV 220

Query: 181 EQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPSSLALESIPEMNENHSGVSLKDSKENF 240
           EQEDKSLLTKWMPLSEKYV+EWVGPGSPRVRSPSSLALESIPEMNEN+SG  LKDSK NF
Sbjct: 221 EQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNF 280

Query: 241 TGSKPENKPLPSRSGNDFMNCNDENLLKIQQEYFVPWHLPYHRAEVSGSSASALASGLAK 300
           TGS PEN PLPS+ GNDF NCNDENL K QQEYFVPWHLPYHRAEVSG+SA ALASGLAK
Sbjct: 281 TGSMPENIPLPSQGGNDFANCNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAK 340

Query: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 353
           KDDKKAAVVYNRYYHVFSEGELERLVSGMD+AVVVDRFYDKSNWC+VLEKT+
Sbjct: 341 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

[Close]

BLAST of CmaCh04G030000 vs. TAIR 10
Match: AT1G36310.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 464.2 bits (1193), Expect = 9.6e-131
Identity = 247/373 (66.22%), Postives = 284/373 (76.14%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSS--SNVQCTPEIEKKYVHRVYDAIAP 60
           M++IKV+S S    + L      ++  S   S SS  S+V+ TPEIEKKYVHRVYDAIAP
Sbjct: 29  MRDIKVKSDSK---EFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHRVYDAIAP 88

Query: 61  HFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDE 120
           HFSSTRFAKWPKVA+FL SLP GS++LDAGCGNGKYLG NP+CFFIGCDIS  LIKIC +
Sbjct: 89  HFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHPLIKICSD 148

Query: 121 RGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVW 180
           +G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRR+KAIEEL+RVVK GG VLITVW
Sbjct: 149 KGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGGFVLITVW 208

Query: 181 AVEQEDKSLLTKWMPLSEKYVDEWVGPG----SPRVRSPSSLALESIPEM---------- 240
           A EQED SLLTKW PLS KYV+EWVGPG    SPRVR+    +LESIPE           
Sbjct: 209 AAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVSTKEQKVE 268

Query: 241 NENHSGV-SLKDSKENFTGSK-----PENK-PLPSRSGNDFMNCNDENLLKIQQEYFVPW 300
           N    G+ S+ +S+E+    K     PE K  +  +     +  + E L K QQEYFVPW
Sbjct: 269 NSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQQEYFVPW 328

Query: 301 HLPYHRAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDR 351
           HLPYHRAEVSG+SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ +A++VDR
Sbjct: 329 HLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVGNAMIVDR 388

[Close]

BLAST of CmaCh04G030000 vs. TAIR 10
Match: AT1G36310.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 464.2 bits (1193), Expect = 9.6e-131
Identity = 247/373 (66.22%), Postives = 284/373 (76.14%), Query Frame = 0


Query: 1   MKEIKVRSGSNADPDLLNDETHAQQLTSANQSCSS--SNVQCTPEIEKKYVHRVYDAIAP 60
           M++IKV+S S    + L      ++  S   S SS  S+V+ TPEIEKKYVHRVYDAIAP
Sbjct: 29  MRDIKVKSDSK---EFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHRVYDAIAP 88

Query: 61  HFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDE 120
           HFSSTRFAKWPKVA+FL SLP GS++LDAGCGNGKYLG NP+CFFIGCDIS  LIKIC +
Sbjct: 89  HFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHPLIKICSD 148

Query: 121 RGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVW 180
           +G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRR+KAIEEL+RVVK GG VLITVW
Sbjct: 149 KGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGGFVLITVW 208

Query: 181 AVEQEDKSLLTKWMPLSEKYVDEWVGPG----SPRVRSPSSLALESIPEM---------- 240
           A EQED SLLTKW PLS KYV+EWVGPG    SPRVR+    +LESIPE           
Sbjct: 209 AAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVSTKEQKVE 268

Query: 241 NENHSGV-SLKDSKENFTGSK-----PENK-PLPSRSGNDFMNCNDENLLKIQQEYFVPW 300
           N    G+ S+ +S+E+    K     PE K  +  +     +  + E L K QQEYFVPW
Sbjct: 269 NSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQQEYFVPW 328

Query: 301 HLPYHRAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDDAVVVDR 351
           HLPYHRAEVSG+SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ +A++VDR
Sbjct: 329 HLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVGNAMIVDR 388

The following BLAST results are available for this feature:

BLAST of CmaCh04G030000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94A09	1.4e-129	66.22	tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX...	[more]
Q80Y20	4.0e-57	39.74	Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 P...	[more]
Q07G10	1.5e-56	41.56	Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alk...	[more]
A1A4L5	1.1e-54	38.49	Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2...	[more]
Q95K79	1.6e-53	37.94	Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=AL...	[more]

BLAST of CmaCh04G030000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JQT2	2.1e-202	100.00	alkylated DNA repair protein alkB homolog 8-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1HLF5	1.1e-201	99.43	alkylated DNA repair protein alkB homolog 8-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1JY21	2.6e-184	90.63	alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima ...	[more]
A0A6J1K1M9	2.6e-184	90.63	alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita maxima ...	[more]
A0A6J1FLV5	2.9e-183	90.34	alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschat...	[more]

BLAST of CmaCh04G030000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022990695.1	4.4e-202	100.00	alkylated DNA repair protein alkB homolog 8-like [Cucurbita maxima] >XP_02299069...	[more]
XP_022964628.1	2.2e-201	99.43	alkylated DNA repair protein alkB homolog 8-like [Cucurbita moschata] >XP_022964...	[more]
KAG6602706.1	1.1e-200	99.43	tRNA (carboxymethyluridine(34)-5-O)-methyltransferase, partial [Cucurbita argyro...	[more]
XP_023518340.1	2.8e-196	96.88	alkylated DNA repair protein alkB homolog 8-like [Cucurbita pepo subsp. pepo]	[more]
XP_022993245.1	5.4e-184	90.63	alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita maxima]	[more]

BLAST of CmaCh04G030000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36310.1	9.6e-131	66.22	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G36310.2	9.6e-131	66.22	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013216	Methyltransferase type 11	PFAM	PF08241	Methyltransf_11	coord: 85..175 e-value: 5.1E-14 score: 52.7
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 60..351 e-value: 8.6E-59 score: 200.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 227..256
None	No IPR available	PANTHER	PTHR13069	UNCHARACTERIZED	coord: 267..345 coord: 30..238
None	No IPR available	PANTHER	PTHR13069:SF33	BNAA05G09210D PROTEIN	coord: 267..345 coord: 30..238
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 84..180 e-value: 3.39318E-10 score: 54.7434
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 37..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G030000.1	CmaCh04G030000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0000049	tRNA binding