CmaCh04G029760 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G029760
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4-like
LocationCma_Chr04: 19524017 .. 19526647 (-)
RNA-Seq ExpressionCmaCh04G029760
SyntenyCmaCh04G029760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCGGGTGAATGAGAAGCTTGTGGCCTTTTTGAACAACCGATGGTTGGTTTTTGTGGCTGCAATATGGGTACAATCTTGCGCCGGAATTGGGTATCTTTTCGGAAGCATATCGCCGGTGATCAAGACGAATTTGAGTTATAATCAGAAGCAAATTGCGCGGCTCGGTGTCGCGAAGGATCTTGGCGATAGCGTTGGCATTTTGGCCGGAACCTTGTCGGAGATATTGCCGTTCTGGGGAACTTTATTAGTCGGTGCTCTCAATAACTTTATTGGGTATGGTTGGGTTTGGCTCATCGTCACTGGTCGAGCTCCTGTTTTGCCTCTCTGGGCTGTAAGTTTCGTATCTTTCCCTCTTTTTTCTTTCTTTTAATTCCTCTATTTGAGTTTCTATTCGGATTCCATTTCCATTTCTTTTTGGGTTTGGATCTTCGTCTCTCGATAGTTCGAAACGGATATCCATGATCAGATCCTCATTGAGAATGGTTTCAAAATTTGCAAAGTGGTCAACGAGTTGATTACTCTCAAAAGCGCATTTGGGTGTGGGGGGATGATTTTTTCTTTAGATGGGTTGTCGAAAAACTCGGCGGGATGCAGCTTATCTGTTTGATAAAATGTTTCACTGAGATTATAGAAGCAATTTTGTTGAATATTCAATGATGGAAACACTGATTGCTATGAAAATACTTTACAAATAGATTTGCTGAATCTATATGTTGATTGAGGCTATTAAAAAGTTGTTTTGAATGTTCCTGAATCAATCTTAGCCTGTGCGTTGGCTACTGGGCCACTCTAATCTGTTGAAGTTTAAGAACAATTTCTGGTTGGTTAAGTATGGATATCATTGTGCAGATGTGCGTTCTTGTGTTTGTGGGAACGAATGGGGAGACATACTTCAACACAGTTTCTTTGGTTTCTTGCGTACAAAACTTTCCGAAGAGTAGGGGTCCGGTGGTAGGGATCTTGAAAGGCTTTGCTGGTTTAAGTGGTGCAATTTTGACTCAAATATATGCAATCATGCATTTCCCTGATTCAGCCAATCTTATTTTCATGATAGCAGTAGGTCCTGCTCTTGTGGCTATTGGTATGATGTTCTTTATCCGACCCGTTGCTGGTCATCGACAAGTGAGGCCGTCCGATGGCATGAGCTTTAGTAGTGTTTATGGTGTCTGCCTCCTTTTGGCTGCATATCTAATGGGAGTCATGCTTGTTGAGGATCTTGTTGATTTGAGCCCCACTGTGATAGCCATTTTCACTGCTGTTATGTTTGTTATCCTTCTTACTCCCTTTTTGATTCCTGTGATATTGACTTTTAGCTCGGAAACAATGGCGTATCCCGAGCAAGAGGCTCTCTTGCAACAGTCACCTAAACAGGAGCCTGCTAGATCTGAACCAGATGGTCATGAAGTGATATTTAGTGAGGTCGAAGATGAAAAATCGGAGGGAGAGGACTTACTCCCTGCATTGGAGCGACAAAAACGCATTGCTCAGTTGCAAGCAAGACTATTGCAGGCAGCTGCAGAAGGAGCAGTACGAGTCAAGAGGAGGAAGGGTCCTCGTAGAGGGGAGGACTTTACTTTGGGTCAAGCTTTGATCAAAGCAGACTTTTGGCTTATATTCTTCTCCCTTCTCCTTGGCTCTGGAACTGGGTTGACCGTGATCGATAACCTCGGACAGATGAGTCAGTCTTTAGGCTATGATAATACGCATATTTTTGTATCCCTGATCAGCATATGGAACTTTCTTGGCCGGGTTGGTGGCGGTTACTTCTCTGAGATCGTTGTGAGGTACGAAAATGATTTCAGTTAGAGTCATAAAACTGTATACTTCTCTTAATACTTTATACTTTTGTTGAACAGAGATTATGCTTATCCAAGGCCCATTGCTATGGCAACTGCACAATTCCTTATGATATTTGGGCATATCTTCATCGGTATGGGATGGCCCGGGGCAATGTACATTGGCACACTCATCACGGGGCTCGGATACGGGGCTCACTGGGCAATTGTGCCTGCTACTGCCTCGGAGTTGTTTGGGTTGAAAAAGTTCGGGGCTTTATACAACTTCCTGACTCTCTCAACTCCGATGGGATCTCTGATATTTTCGGGCCTAATTGCGAGCAGTATATATGATAGTGAAGCAGAGAAGCAAGCACATAATGGTCTCTCACAGCTGCAGAGCTCCTCATCAGTTTGGTTCAGCAGACTTCATGTCGATGCACCGCTCAAGTGCGACGGTGCTATATGTTTCTTTCTAACTTGTATGATAATGGCTGGATTCTGTGCTATTGCAGGCATGTTAAGTTTGATTTTGGTGCACCGGACAAAAGGTGTGTACTACAACCTGTACGGAAAATCCCGAGCTTCGACGCTCTCCTAGACCGGTTTCATTCGTCGTGTTATTCTTTTTCATGTTTTTTATTTTGAGATCGAGTGAATTATTTGCTCGGATGGCATTGCAAGAACGACACGAGAACCCGAAATGGATATTTGCTTCACTGTTACACTTAAGAATTCTTCACCCTTTGATGTAAAAGCTTTTTCCTGTGTGTTGCAGACTGCAGTCGGGTGGAAGAGAAACAGTCATCTTCATGTGCCACATTCCTCCCTTCCTCCCTTAAAAAAAAAAA

mRNA sequence

ATGGGTCGGGTGAATGAGAAGCTTGTGGCCTTTTTGAACAACCGATGGTTGGTTTTTGTGGCTGCAATATGGGTACAATCTTGCGCCGGAATTGGGTATCTTTTCGGAAGCATATCGCCGGTGATCAAGACGAATTTGAGTTATAATCAGAAGCAAATTGCGCGGCTCGGTGTCGCGAAGGATCTTGGCGATAGCGTTGGCATTTTGGCCGGAACCTTGTCGGAGATATTGCCGTTCTGGGGAACTTTATTAGTCGGTGCTCTCAATAACTTTATTGGGTATGGTTGGGTTTGGCTCATCGTCACTGGTCGAGCTCCTGTTTTGCCTCTCTGGGCTATGTGCGTTCTTGTGTTTGTGGGAACGAATGGGGAGACATACTTCAACACAGTTTCTTTGGTTTCTTGCGTACAAAACTTTCCGAAGAGTAGGGGTCCGGTGGTAGGGATCTTGAAAGGCTTTGCTGGTTTAAGTGGTGCAATTTTGACTCAAATATATGCAATCATGCATTTCCCTGATTCAGCCAATCTTATTTTCATGATAGCAGTAGGTCCTGCTCTTGTGGCTATTGGTATGATGTTCTTTATCCGACCCGTTGCTGGTCATCGACAAGTGAGGCCGTCCGATGGCATGAGCTTTAGTAGTGTTTATGGTGTCTGCCTCCTTTTGGCTGCATATCTAATGGGAGTCATGCTTGTTGAGGATCTTGTTGATTTGAGCCCCACTGTGATAGCCATTTTCACTGCTGTTATGTTTGTTATCCTTCTTACTCCCTTTTTGATTCCTGTGATATTGACTTTTAGCTCGGAAACAATGGCGTATCCCGAGCAAGAGGCTCTCTTGCAACAGTCACCTAAACAGGAGCCTGCTAGATCTGAACCAGATGGTCATGAAGTGATATTTAGTGAGGTCGAAGATGAAAAATCGGAGGGAGAGGACTTACTCCCTGCATTGGAGCGACAAAAACGCATTGCTCAGTTGCAAGCAAGACTATTGCAGGCAGCTGCAGAAGGAGCAGTACGAGTCAAGAGGAGGAAGGGTCCTCGTAGAGGGGAGGACTTTACTTTGGGTCAAGCTTTGATCAAAGCAGACTTTTGGCTTATATTCTTCTCCCTTCTCCTTGGCTCTGGAACTGGGTTGACCGTGATCGATAACCTCGGACAGATGAGTCAGTCTTTAGGCTATGATAATACGCATATTTTTGTATCCCTGATCAGCATATGGAACTTTCTTGGCCGGGTTGGTGGCGGTTACTTCTCTGAGATCGTTGTGAGAGATTATGCTTATCCAAGGCCCATTGCTATGGCAACTGCACAATTCCTTATGATATTTGGGCATATCTTCATCGGTATGGGATGGCCCGGGGCAATGTACATTGGCACACTCATCACGGGGCTCGGATACGGGGCTCACTGGGCAATTGTGCCTGCTACTGCCTCGGAGTTGTTTGGGTTGAAAAAGTTCGGGGCTTTATACAACTTCCTGACTCTCTCAACTCCGATGGGATCTCTGATATTTTCGGGCCTAATTGCGAGCAGTATATATGATAGTGAAGCAGAGAAGCAAGCACATAATGGTCTCTCACAGCTGCAGAGCTCCTCATCAGTTTGGTTCAGCAGACTTCATGTCGATGCACCGCTCAAGTGCGACGGTGCTATATGTTTCTTTCTAACTTGTATGATAATGGCTGGATTCTGTGCTATTGCAGGCATGTTAAGTTTGATTTTGGTGCACCGGACAAAAGGTGTGTACTACAACCTGTACGGAAAATCCCGAGCTTCGACGCTCTCCTAGACCGGTTTCATTCGTCGTGTTATTCTTTTTCATGTTTTTTATTTTGAGATCGAGTGAATTATTTGCTCGGATGGCATTGCAAGAACGACACGAGAACCCGAAATGGATATTTGCTTCACTGTTACACTTAAGAATTCTTCACCCTTTGATGTAAAAGCTTTTTCCTGTGTGTTGCAGACTGCAGTCGGGTGGAAGAGAAACAGTCATCTTCATGTGCCACATTCCTCCCTTCCTCCCTTAAAAAAAAAAA

Coding sequence (CDS)

ATGGGTCGGGTGAATGAGAAGCTTGTGGCCTTTTTGAACAACCGATGGTTGGTTTTTGTGGCTGCAATATGGGTACAATCTTGCGCCGGAATTGGGTATCTTTTCGGAAGCATATCGCCGGTGATCAAGACGAATTTGAGTTATAATCAGAAGCAAATTGCGCGGCTCGGTGTCGCGAAGGATCTTGGCGATAGCGTTGGCATTTTGGCCGGAACCTTGTCGGAGATATTGCCGTTCTGGGGAACTTTATTAGTCGGTGCTCTCAATAACTTTATTGGGTATGGTTGGGTTTGGCTCATCGTCACTGGTCGAGCTCCTGTTTTGCCTCTCTGGGCTATGTGCGTTCTTGTGTTTGTGGGAACGAATGGGGAGACATACTTCAACACAGTTTCTTTGGTTTCTTGCGTACAAAACTTTCCGAAGAGTAGGGGTCCGGTGGTAGGGATCTTGAAAGGCTTTGCTGGTTTAAGTGGTGCAATTTTGACTCAAATATATGCAATCATGCATTTCCCTGATTCAGCCAATCTTATTTTCATGATAGCAGTAGGTCCTGCTCTTGTGGCTATTGGTATGATGTTCTTTATCCGACCCGTTGCTGGTCATCGACAAGTGAGGCCGTCCGATGGCATGAGCTTTAGTAGTGTTTATGGTGTCTGCCTCCTTTTGGCTGCATATCTAATGGGAGTCATGCTTGTTGAGGATCTTGTTGATTTGAGCCCCACTGTGATAGCCATTTTCACTGCTGTTATGTTTGTTATCCTTCTTACTCCCTTTTTGATTCCTGTGATATTGACTTTTAGCTCGGAAACAATGGCGTATCCCGAGCAAGAGGCTCTCTTGCAACAGTCACCTAAACAGGAGCCTGCTAGATCTGAACCAGATGGTCATGAAGTGATATTTAGTGAGGTCGAAGATGAAAAATCGGAGGGAGAGGACTTACTCCCTGCATTGGAGCGACAAAAACGCATTGCTCAGTTGCAAGCAAGACTATTGCAGGCAGCTGCAGAAGGAGCAGTACGAGTCAAGAGGAGGAAGGGTCCTCGTAGAGGGGAGGACTTTACTTTGGGTCAAGCTTTGATCAAAGCAGACTTTTGGCTTATATTCTTCTCCCTTCTCCTTGGCTCTGGAACTGGGTTGACCGTGATCGATAACCTCGGACAGATGAGTCAGTCTTTAGGCTATGATAATACGCATATTTTTGTATCCCTGATCAGCATATGGAACTTTCTTGGCCGGGTTGGTGGCGGTTACTTCTCTGAGATCGTTGTGAGAGATTATGCTTATCCAAGGCCCATTGCTATGGCAACTGCACAATTCCTTATGATATTTGGGCATATCTTCATCGGTATGGGATGGCCCGGGGCAATGTACATTGGCACACTCATCACGGGGCTCGGATACGGGGCTCACTGGGCAATTGTGCCTGCTACTGCCTCGGAGTTGTTTGGGTTGAAAAAGTTCGGGGCTTTATACAACTTCCTGACTCTCTCAACTCCGATGGGATCTCTGATATTTTCGGGCCTAATTGCGAGCAGTATATATGATAGTGAAGCAGAGAAGCAAGCACATAATGGTCTCTCACAGCTGCAGAGCTCCTCATCAGTTTGGTTCAGCAGACTTCATGTCGATGCACCGCTCAAGTGCGACGGTGCTATATGTTTCTTTCTAACTTGTATGATAATGGCTGGATTCTGTGCTATTGCAGGCATGTTAAGTTTGATTTTGGTGCACCGGACAAAAGGTGTGTACTACAACCTGTACGGAAAATCCCGAGCTTCGACGCTCTCCTAG

Protein sequence

MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAKDLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVGTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMIAVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSPTVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIFSEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSEIVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRLHVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS
Homology
BLAST of CmaCh04G029760 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 4.3e-27
Identity = 142/578 (24.57%), Postives = 251/578 (43.43%), Query Frame = 0

Query: 15  RWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAKDLGDSVGILAGTLS 74
           +W V VAAIW+Q+  G  + F + S  +K+ L  +Q ++  L VA DLG + G  +G   
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 75  EILPFWGTLLVGALNNFIGYGWVWLIVTG--RAPVLPLWAMCVLVFVGTNGETYFNTVSL 134
              P    L   A   F+GYG  WL++T     P   ++  C+L  +      +FNT   
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGLSI---CWFNTACF 162

Query: 135 VSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI-AVGPALVAIGM 194
           + C+++FP +R   + +   F G+S A+ +  +  ++ P S+NL  ++ ++ P +V+   
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAIN-PSSSNLYLLLNSLVPLVVSFAA 222

Query: 195 MFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSPTVIAIFTAVMF 254
           ++ +            D     S     L + A +    L   L+  S T  A    +  
Sbjct: 223 LYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHL---LLSSSSTSSARLNFIGA 282

Query: 255 VILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVI-FSEVEDEKSEG 314
           V+LL   L   +L ++ +    P   A L           E  G+ ++   E++++K+  
Sbjct: 283 VVLLVFPLCAPLLVYARDYF-LPVINARLNH---------ESSGYVMLNIDELKNQKTSV 342

Query: 315 EDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKADFWLIFFS 374
                                +   E     K     R G++ +    + + +FWL + +
Sbjct: 343 SS-------------------KTGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIA 402

Query: 375 LLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSEIVVRDYAYPR 434
              G   GL   +NLGQ++QSLG ++T + V++ S ++F GR+      + + + +   R
Sbjct: 403 YFCGGTIGLVYSNNLGQIAQSLGQNSTTL-VTIYSSFSFFGRLLSA-APDFMHKRFRLTR 462

Query: 435 P--IAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFGLKKFG 494
               A+A     + F  + +      A+   T + GL  G  +A   +  S+LFG    G
Sbjct: 463 TGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVG 522

Query: 495 ALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRLHVDAPLKC 554
             +N L  + P+GSL++ G IA+SIY++ A       ++ + S S V            C
Sbjct: 523 VNHNILITNIPIGSLLY-GYIAASIYEANASPD----ITPIVSDSIV------------C 565

Query: 555 DGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNL 587
            G  C+F T +       +  + SL L  RTK VY+ L
Sbjct: 583 IGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRL 565

BLAST of CmaCh04G029760 vs. ExPASy TrEMBL
Match: A0A6J1JLV2 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487059 PE=4 SV=1)

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
           TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CmaCh04G029760 vs. ExPASy TrEMBL
Match: A0A6J1E533 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111430673 PE=4 SV=1)

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 592/596 (99.33%), Postives = 594/596 (99.66%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIGMMFFIRPVAGHRQVRPSDG+SFSSVYGVCLLLAAYLMGVMLVEDLVDLSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGVSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
           TVIAIFTAVMFVILLTPFLIPVILTFSSET AYPEQEALLQQSPKQEPARSEPDGHEVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGY+NTHIFVSLISIWNFLGRVGGGYFSE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYNNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CmaCh04G029760 vs. ExPASy TrEMBL
Match: A0A5A7UN83 (Protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1154G00120 PE=4 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 4.0e-302
Identity = 535/596 (89.77%), Postives = 566/596 (94.97%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISPVIKTNLSYNQ+Q++RLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++N +GYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H PDSANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
           TVI IFT VMFVILLTPFLIPV LTFSSET  Y EQEALL  S K+EPAR+EPDG+EVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNF+TLSTPMGSLIFSGLIASSIYDSEAEKQA N L+Q QSSSS+WF+RL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           + + P KC+GAICFFLTCMIMAGFCAIAG+LSLILV+RTKGVY+NLYGKSR STLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of CmaCh04G029760 vs. ExPASy TrEMBL
Match: A0A1S3B357 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485475 PE=4 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 4.0e-302
Identity = 535/596 (89.77%), Postives = 566/596 (94.97%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISPVIKTNLSYNQ+Q++RLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++N +GYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H PDSANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
           TVI IFT VMFVILLTPFLIPV LTFSSET  Y EQEALL  S K+EPAR+EPDG+EVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNF+TLSTPMGSLIFSGLIASSIYDSEAEKQA N L+Q QSSSS+WF+RL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           + + P KC+GAICFFLTCMIMAGFCAIAG+LSLILV+RTKGVY+NLYGKSR STLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of CmaCh04G029760 vs. ExPASy TrEMBL
Match: A0A0A0LQ60 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G406130 PE=4 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 2.3e-297
Identity = 525/596 (88.09%), Postives = 557/596 (93.46%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISP+IKTNLSYNQ+QI+RLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++NF+GYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI H P+SANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDGMSF+SVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
            VI IFT VMFVILLTPF IPV LT SSE   Y EQEALL  S K+EPAR+EPDG+EVIF
Sbjct: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNF+TLSTPMGSL+FSGLIASSIYDSEAEKQA N L+Q QSSSS WF+RL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           + + P KC+GAICFFLTCMIM GFCAIA +LSLILVHRTKGVY+NLYGKSR STLS
Sbjct: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 596

BLAST of CmaCh04G029760 vs. NCBI nr
Match: XP_022990046.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
           TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CmaCh04G029760 vs. NCBI nr
Match: KAG6602685.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 593/596 (99.50%), Postives = 594/596 (99.66%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIGMMFFIRPVAGHRQVRPSDG+SFSSVYGVCLLLAAYLMGVMLVEDLVDLSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGVSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
           TVIAIFTAVMFVILLTPFLIPVILTFSSET AYPEQEALLQQSPKQEPARSEPDGHEVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CmaCh04G029760 vs. NCBI nr
Match: XP_023544039.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 592/596 (99.33%), Postives = 593/596 (99.50%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGRVNEKLVAFLNNRWLVFVAAIW+QSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWIQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
           TVIAIFTAVMFVILLTPFLIPVILTFSSET AYPEQEALLQQSPK EPARSEPDGHEVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKHEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CmaCh04G029760 vs. NCBI nr
Match: KAG7033371.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 592/596 (99.33%), Postives = 593/596 (99.50%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGRVNEKLV FLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK
Sbjct: 1   MGRVNEKLVVFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIGMMFFIRPVAGHRQVRPSDG+SFSSVYGVCLLLAAYLMGVMLVEDLVDLSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGVSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
           TVIAIFTAVMFVILLTPFLIPVILTFSSET AYPEQEALLQQSPKQEPARSEPDGHEVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CmaCh04G029760 vs. NCBI nr
Match: XP_022922791.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita moschata])

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 592/596 (99.33%), Postives = 594/596 (99.66%), Query Frame = 0

Query: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60
           MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240
           AVGPALVAIGMMFFIRPVAGHRQVRPSDG+SFSSVYGVCLLLAAYLMGVMLVEDLVDLSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGVSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300
           TVIAIFTAVMFVILLTPFLIPVILTFSSET AYPEQEALLQQSPKQEPARSEPDGHEVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420
           KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGY+NTHIFVSLISIWNFLGRVGGGYFSE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYNNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540
           LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 597
           HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of CmaCh04G029760 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 822.8 bits (2124), Expect = 1.8e-238
Identity = 419/588 (71.26%), Postives = 490/588 (83.33%), Query Frame = 0

Query: 6   EKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAKDLGDS 65
           EK V+F+NNRWLVFVAA+W+QSCAGIGYLFGSISPVIK++L+YNQK+++RLGVAKDLGDS
Sbjct: 7   EKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDS 66

Query: 66  VGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVGTNGET 125
           VG +AGTLSEILP W  LLVGA+ N IGYGWVWLIVTGRAP+LPLWAMCVL+FVG NGET
Sbjct: 67  VGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGET 126

Query: 126 YFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMIAVGPA 185
           YFNT +LVS VQNFPKSRGPVVGILKGFAGL GAI++QIY ++H  + A+LI M+AV PA
Sbjct: 127 YFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPA 186

Query: 186 LVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSPTVIAI 245
           +V + +MFFIRPV GH+Q+RP+DG SF+ +YGVCLLLAAYLM VML++DLV +S  VI +
Sbjct: 187 VVVVCLMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITV 246

Query: 246 FTAVMFVILLTPFLIPVILTFSSETMAYPE--QEALLQQSPKQEPARSEPDGHEVIFSEV 305
           FT V+FVIL+ P L+P++ +F +ET    +  +E L+ +   QEP    PD   +I SEV
Sbjct: 247 FTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPD---LILSEV 306

Query: 306 EDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKAD 365
           EDEK +  DLLPA ER KRIA LQA+L+QAAAEGAVRV RR+GP RGEDFTL QAL+KAD
Sbjct: 307 EDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKAD 366

Query: 366 FWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSEIVV 425
           FWLIFFSLLLGSG+GLTVIDNLGQMSQSLGYDNTH+ VS+ISIWNFLGR+GGGYFSE+VV
Sbjct: 367 FWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVV 426

Query: 426 RDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFG 485
           RDYAYPRP+AMA AQ +M  GHIF   GWPGAMYIGTL+ GLGYGAHWAIVPATASELFG
Sbjct: 427 RDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFG 486

Query: 486 LKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRLHVD 545
           LKKFGALYNFLTL+ P GSL+FSG+IASSIYD EAE+QAH              S    D
Sbjct: 487 LKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQAHG-------------SVFDPD 546

Query: 546 APLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSR 592
             L+C+G+ICFFLT +IM+GFC IA MLS+ILV RTK VY +LYGK+R
Sbjct: 547 DALRCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVYTHLYGKTR 578

BLAST of CmaCh04G029760 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 817.0 bits (2109), Expect = 9.9e-237
Identity = 418/590 (70.85%), Postives = 490/590 (83.05%), Query Frame = 0

Query: 6   EKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAKDLGDS 65
           E++ +F+NNRWLVFVAA+W+QSCAGIGYLFGSISPVIK++L+YNQKQ++RLGVAKDLGDS
Sbjct: 7   ERVKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDS 66

Query: 66  VGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVGTNGET 125
           VG LAGTLSEILP W  LLVG++ N +GYGWVWLIVTGRAP+LPLWAMC+L+FVG NGET
Sbjct: 67  VGFLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGET 126

Query: 126 YFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMIAVGPA 185
           YFNT +LVS VQNFPKSRGPVVGILKGFAGL GAIL+Q+Y ++H  D A+LIFM+AV P+
Sbjct: 127 YFNTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPS 186

Query: 186 LVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSPTVIAI 245
           +V + +MFFIRPV GHRQ+R SD  SF+ +Y VC+LLAAYLM VMLVED +DLS ++I  
Sbjct: 187 VVVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIA 246

Query: 246 FTAVMFVILLTPFLIPVILT-FSSETMAYPE-QEALLQQSPKQEPARS-EPD-GHEVIFS 305
           FT V+F ILL P  IP+  + F++ T      +E LL     Q+P +S  PD G E+IFS
Sbjct: 247 FTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFS 306

Query: 306 EVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIK 365
           EVEDEK +  DLLPA+ER KRIAQLQA+L+QAAAEGAVRVKRR+GP RGEDFTL QAL+K
Sbjct: 307 EVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVK 366

Query: 366 ADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSEI 425
           ADFWLIFFSLLLGSG+GLTVIDNLGQMSQSLGYDNTH+FVS+ISIWNFLGR+GGGYFSE+
Sbjct: 367 ADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSEL 426

Query: 426 VVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASEL 485
           +VRDYAYPRP+A+A AQ +M  GHIF   GWPGAM+IGTL+ GLGYGAHWAIVPATASEL
Sbjct: 427 IVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASEL 486

Query: 486 FGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRLH 545
           FGLKKFGALYNFLTL+ P GSL+FSGLIASSIYD EAE+QA   L              +
Sbjct: 487 FGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSL-------------FN 546

Query: 546 VDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSR 592
            D  L+C G+IC+FLT +IM+GFC IA  LS+ILV RTK VY NLYGK+R
Sbjct: 547 PDDVLRCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLYGKTR 583

BLAST of CmaCh04G029760 vs. TAIR 10
Match: AT3G01930.1 (Major facilitator superfamily protein )

HSP 1 Score: 632.1 bits (1629), Expect = 4.5e-181
Identity = 333/483 (68.94%), Postives = 390/483 (80.75%), Query Frame = 0

Query: 113 MCVLVFVGTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPD 172
           MC+L+FVG NGETYFNT +LVS VQNFPKSRGPVVGILKGFAGL GAIL+Q+Y ++H  D
Sbjct: 1   MCILIFVGNNGETYFNTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSD 60

Query: 173 SANLIFMIAVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLV 232
            A+LIFM+AV P++V + +MFFIRPV GHRQ+R SD  SF+ +Y VC+LLAAYLM VMLV
Sbjct: 61  RASLIFMVAVAPSVVVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLV 120

Query: 233 EDLVDLSPTVIAIFTAVMFVILLTPFLIPVILT-FSSETMAYPE-QEALLQQSPKQEPAR 292
           ED +DLS ++I  FT V+F ILL P  IP+  + F++ T      +E LL     Q+P +
Sbjct: 121 EDFIDLSHSIIIAFTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQ 180

Query: 293 S-EPD-GHEVIFSEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPR 352
           S  PD G E+IFSEVEDEK +  DLLPA+ER KRIAQLQA+L+QAAAEGAVRVKRR+GP 
Sbjct: 181 STTPDHGPELIFSEVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPH 240

Query: 353 RGEDFTLGQALIKADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWN 412
           RGEDFTL QAL+KADFWLIFFSLLLGSG+GLTVIDNLGQMSQSLGYDNTH+FVS+ISIWN
Sbjct: 241 RGEDFTLTQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWN 300

Query: 413 FLGRVGGGYFSEIVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYG 472
           FLGR+GGGYFSE++VRDYAYPRP+A+A AQ +M  GHIF   GWPGAM+IGTL+ GLGYG
Sbjct: 301 FLGRIGGGYFSELIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYG 360

Query: 473 AHWAIVPATASELFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQ 532
           AHWAIVPATASELFGLKKFGALYNFLTL+ P GSL+FSGLIASSIYD EAE+QA   L  
Sbjct: 361 AHWAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSL-- 420

Query: 533 LQSSSSVWFSRLHVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYG 592
                       + D  L+C G+IC+FLT +IM+GFC IA  LS+ILV RTK VY NLYG
Sbjct: 421 -----------FNPDDVLRCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLYG 470

BLAST of CmaCh04G029760 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 513.1 bits (1320), Expect = 3.1e-145
Identity = 279/583 (47.86%), Postives = 382/583 (65.52%), Query Frame = 0

Query: 7   KLVAFLNNRWLVFVAAIWVQSCAGIGYLF-GSISPVIKTNLSYNQKQIARLGVAKDLGDS 66
           KL   +N+RWLVFV A+WVQS AG+GYLF GS+SP IKT+L YNQKQIA LGVAK+LGD+
Sbjct: 8   KLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDA 67

Query: 67  VGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVGTNGET 126
           +G ++G LSE+ P W  LLVGA  N  GYG VWL+VTG+ P LPLW + V +FVGTNGET
Sbjct: 68  IGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGET 127

Query: 127 YFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMIAVGPA 186
           Y+NT SLVSC+ NFP+SRGPVVGILKGF+GLSGAILTQ+Y + +    +++I M+A+GP 
Sbjct: 128 YYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPP 187

Query: 187 LVAIGMMFFIRPV-AGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSPTVIA 246
           +V + ++F +RPV    R    SD + F ++YG C++LA YL+G+++++ + D++ T+I 
Sbjct: 188 VVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIIT 247

Query: 247 IFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIFSEVE 306
              A++ + ++ P L+P    F S              S K E   S  D HE       
Sbjct: 248 TSGAILVIFMVVPVLVPFSSVFISGNNV---------TSVKPEEGTSNVDQHEA------ 307

Query: 307 DEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKADF 366
                       +ER  R  +                 +++ P  GEDFTL QAL +ADF
Sbjct: 308 ---------RTLIERSDRPPE-----------------KKRAPCIGEDFTLLQALGQADF 367

Query: 367 WLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSEIVVR 426
           WLIF SL+LG G+G+T+IDNLGQ+  SLGY NT IFVSLISI NFLGRV GGYFSE+++R
Sbjct: 368 WLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLISISNFLGRVAGGYFSELIIR 427

Query: 427 DYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFGL 486
             + PR +AM+  Q +M  G I+  + WPG +Y+ T++ G+GYGAHWAI PA+ S++FGL
Sbjct: 428 KLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGL 487

Query: 487 KKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRLHVDA 546
           K FG+LYNF   + P+GS +FSG+IAS+IYD  A KQA    ++ +S             
Sbjct: 488 KSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQA-GPTTETES------------- 534

Query: 547 PLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLY 588
            L C G++C+ +TC +M+  C +A +LSL +V+RT+  Y  L+
Sbjct: 548 -LVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRLH 534

BLAST of CmaCh04G029760 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 513.1 bits (1320), Expect = 3.1e-145
Identity = 279/583 (47.86%), Postives = 382/583 (65.52%), Query Frame = 0

Query: 7   KLVAFLNNRWLVFVAAIWVQSCAGIGYLF-GSISPVIKTNLSYNQKQIARLGVAKDLGDS 66
           KL   +N+RWLVFV A+WVQS AG+GYLF GS+SP IKT+L YNQKQIA LGVAK+LGD+
Sbjct: 8   KLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDA 67

Query: 67  VGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVGTNGET 126
           +G ++G LSE+ P W  LLVGA  N  GYG VWL+VTG+ P LPLW + V +FVGTNGET
Sbjct: 68  IGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGET 127

Query: 127 YFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMIAVGPA 186
           Y+NT SLVSC+ NFP+SRGPVVGILKGF+GLSGAILTQ+Y + +    +++I M+A+GP 
Sbjct: 128 YYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPP 187

Query: 187 LVAIGMMFFIRPV-AGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSPTVIA 246
           +V + ++F +RPV    R    SD + F ++YG C++LA YL+G+++++ + D++ T+I 
Sbjct: 188 VVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIIT 247

Query: 247 IFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIFSEVE 306
              A++ + ++ P L+P    F S              S K E   S  D HE       
Sbjct: 248 TSGAILVIFMVVPVLVPFSSVFISGNNV---------TSVKPEEGTSNVDQHEA------ 307

Query: 307 DEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKADF 366
                       +ER  R  +                 +++ P  GEDFTL QAL +ADF
Sbjct: 308 ---------RTLIERSDRPPE-----------------KKRAPCIGEDFTLLQALGQADF 367

Query: 367 WLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSEIVVR 426
           WLIF SL+LG G+G+T+IDNLGQ+  SLGY NT IFVSLISI NFLGRV GGYFSE+++R
Sbjct: 368 WLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLISISNFLGRVAGGYFSELIIR 427

Query: 427 DYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFGL 486
             + PR +AM+  Q +M  G I+  + WPG +Y+ T++ G+GYGAHWAI PA+ S++FGL
Sbjct: 428 KLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGL 487

Query: 487 KKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRLHVDA 546
           K FG+LYNF   + P+GS +FSG+IAS+IYD  A KQA    ++ +S             
Sbjct: 488 KSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQA-GPTTETES------------- 534

Query: 547 PLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLY 588
            L C G++C+ +TC +M+  C +A +LSL +V+RT+  Y  L+
Sbjct: 548 -LVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRLH 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E14.3e-2724.57Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JLV20.0e+00100.00protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487... [more]
A0A6J1E5330.0e+0099.33protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111430673... [more]
A0A5A7UN834.0e-30289.77Protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3B3574.0e-30289.77protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0LQ602.3e-29788.09Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40613... [more]
Match NameE-valueIdentityDescription
XP_022990046.10.0e+00100.00protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
KAG6602685.10.0e+0099.50Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023544039.10.0e+0099.33protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
KAG7033371.10.0e+0099.33Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022922791.10.0e+0099.33protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G14120.11.8e-23871.26Major facilitator superfamily protein [more]
AT3G01930.29.9e-23770.85Major facilitator superfamily protein [more]
AT3G01930.14.5e-18168.94Major facilitator superfamily protein [more]
AT5G50630.13.1e-14547.86Major facilitator superfamily protein [more]
AT5G50520.13.1e-14547.86Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 313..522
e-value: 1.4E-6
score: 29.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 212..581
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 9..202
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 15..262
e-value: 1.6E-90
score: 303.0
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 3..591
NoneNo IPR availablePANTHERPTHR21576:SF136PROTEIN NUCLEAR FUSION DEFECTIVE 4-LIKEcoord: 3..591
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..28
score: 6.0
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 15..568
e-value: 2.55695E-114
score: 344.235

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G029760.1CmaCh04G029760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane