CmaCh04G029650 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G029650
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Calcium-binding EF-hand family protein
Location	Cma_Chr04: 19477567 .. 19478581 (-)
RNA-Seq Expression	CmaCh04G029650
Synteny	CmaCh04G029650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTAAAAGGGGTAACATGGTAATTTCAATATTGTCAAAAGCCATCCCAAATCTGTTGAATAATACCCCTTTATTTGTGGAAATCACCCTTTTTAGTCTTCAGGTTTTTTTTACGATTCTTTAAACAAAATTTTCTTGGCGGAATTTTAGGGCATTTCCCACGTTTTCCGCCCTTCTCCGTCAATTGTTTATTCTTCAACAATCGCTGAAGAAGCCCACCTTCTTCAAATTCTAGAAATGGTCATCCACCTGCTCCACGAGTTGACGGCAACCACCATTTTCATTTGCCTACTTCTTCGCCGGTCAAACTGCGGTAACGTAACGCGCATTCCCTTTCATATAACTGCATACCCATGTTTCCCCAATTCTCCATTCTCATTTCTCCTAATTCACTCTCGCTCTCCACGCATTCAGAATTATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGTGACGGGAAGCTATCTCTCCCGGAGTTGCAGCGGTGCATGGCGGTCGTCGGGGGTAGCTTGACACTCGAGGAGGCGGAGAAAGTGGTGGAGAATTTGGATTCCGACGGCGATGGGTTGGTGAGCTGGGCTGAATTTGTTGCGTTTATGGAAGAAGTAGGAGAGGAAGAGAAAGTGAGTGATTTGATGGAGGCGTTCAGAATGTATGAAATGGATGGCTGTGGATTCATAACCACTAAGAGCTTGAAGAGGATGCTGAGTAGGCTTGGGGAATCCAGGAGCATCGAGGAATGCAAGAAAATGATAGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTTAAGTTCATGATGTCCTGATTTTCTTCCATTTTTATGTGTTCTGAACTTAGATTTTTGGTCACCAGTTATATACGATTTACTCTTTCTCCTTGTGTTCTTCAATGGTTTGCATTCTCTCTCATAAACTAAAAACTAAGTATAAAATGAACGCTCATCAGCTTACTAAATTACATAAGAACTGCAAGTGAAG

mRNA sequence

ATGTTTAAAAGGGGTAACATGGGCATTTCCCACGTTTTCCGCCCTTCTCCAAATGGTCATCCACCTGCTCCACGAGTTGACGGCAACCACCATTTTCATTTGCCTACTTCTTCGCCGGTCAAACTGCGAATTATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGTGACGGGAAGCTATCTCTCCCGGAGTTGCAGCGGTGCATGGCGGTCGTCGGGGGTAGCTTGACACTCGAGGAGGCGGAGAAAGTGGTGGAGAATTTGGATTCCGACGGCGATGGGTTGGTGAGCTGGGCTGAATTTGTTGCGTTTATGGAAGAAGTAGGAGAGGAAGAGAAAGTGAGTGATTTGATGGAGGCGTTCAGAATGTATGAAATGGATGGCTGTGGATTCATAACCACTAAGAGCTTGAAGAGGATGCTGAGTAGGCTTGGGGAATCCAGGAGCATCGAGGAATGCAAGAAAATGATAGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTTAAGTTCATGATGTCCTGATTTTCTTCCATTTTTATGTGTTCTGAACTTAGATTTTTGGTCACCAGTTATATACGATTTACTCTTTCTCCTTGTGTTCTTCAATGGTTTGCATTCTCTCTCATAAACTAAAAACTAAGTATAAAATGAACGCTCATCAGCTTACTAAATTACATAAGAACTGCAAGTGAAG

Coding sequence (CDS)

ATGTTTAAAAGGGGTAACATGGGCATTTCCCACGTTTTCCGCCCTTCTCCAAATGGTCATCCACCTGCTCCACGAGTTGACGGCAACCACCATTTTCATTTGCCTACTTCTTCGCCGGTCAAACTGCGAATTATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGTGACGGGAAGCTATCTCTCCCGGAGTTGCAGCGGTGCATGGCGGTCGTCGGGGGTAGCTTGACACTCGAGGAGGCGGAGAAAGTGGTGGAGAATTTGGATTCCGACGGCGATGGGTTGGTGAGCTGGGCTGAATTTGTTGCGTTTATGGAAGAAGTAGGAGAGGAAGAGAAAGTGAGTGATTTGATGGAGGCGTTCAGAATGTATGAAATGGATGGCTGTGGATTCATAACCACTAAGAGCTTGAAGAGGATGCTGAGTAGGCTTGGGGAATCCAGGAGCATCGAGGAATGCAAGAAAATGATAGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTTAAGTTCATGATGTCCTGA

Protein sequence

MFKRGNMGISHVFRPSPNGHPPAPRVDGNHHFHLPTSSPVKLRIMDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS

Homology

[Close]

BLAST of CmaCh04G029650 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.2e-30
Identity = 74/139 (53.24%), Postives = 97/139 (69.78%), Query Frame = 0


Query: 49  ALYEKVFKQVDGDGDGKLSLPELQRCM-AVVGGSLTLEEAEKVVENLDSDGDGLVSWAEF 108
           A + +VF   D D DGK+S  EL+ CM A +G  ++ EEAE +V + D+D DGL+   EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 109 --VAFMEEVG-EEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 168
             +A   E+G EEE+   LMEAFRMYEM+G G IT  SLKRMLS+LG  + IEEC+ MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 169 KFDLNSDGVLNFDEFKFMM 184
           +FDL+ DGV++F+EFK MM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

[Close]

BLAST of CmaCh04G029650 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 9.2e-29
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = 0


Query: 54  VFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFM-- 113
           VF  +D + DGK+S  ELQ C++++GG+L+  E E+VV+  D DGDG + + EF+  M  
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 114 EEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 173
           E+  +EE+  +L EAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 174 GVLNFDEFKFMM 184
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of CmaCh04G029650 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 6.0e-28
Identity = 67/136 (49.26%), Postives = 94/136 (69.12%), Query Frame = 0


Query: 54  VFKQVDGDGDGKLSLPELQRCM-AVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFME 113
           +F   D DGDG++S  EL+ CM   +G  ++ EEA ++V ++D+DGDGL+  AEFV  ++
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 114 ----EVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 173
               E  +E + + L EAF MYEM+G G IT  SL+RML RLG  + I++C+ MI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 174 NSDGVLNFDEFKFMMS 185
           N DGVL+FDEFK MM+
Sbjct: 135 NGDGVLSFDEFKIMMN 150

[Close]

BLAST of CmaCh04G029650 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.3e-26
Identity = 64/138 (46.38%), Postives = 89/138 (64.49%), Query Frame = 0


Query: 46  DKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWA 105
           DK    E VF  +D + DG++S  ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 106 EFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 165
           EF   ++   EEEK  +L  AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 166 FDLNSDGVLNFDEFKFMM 184
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of CmaCh04G029650 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.6e-26
Identity = 66/135 (48.89%), Postives = 87/135 (64.44%), Query Frame = 0


Query: 52  EKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFM 111
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V+  D DGDG++   EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 112 ---EEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 171
              +E  EEEK   +MEAFRMY  DG   IT  SLK ML +LGESR+ ++CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 172 NSDGVLNFDEFKFMM 184
           N+DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]

BLAST of CmaCh04G029650 vs. ExPASy TrEMBL
Match: A0A6J1JS06 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111487817 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.7e-71
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
           AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMMS 185
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of CmaCh04G029650 vs. ExPASy TrEMBL
Match: A0A6J1GWQ9 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111457555 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.7e-70
Identity = 139/140 (99.29%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
           AEFVAFME VGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMMS 185
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of CmaCh04G029650 vs. ExPASy TrEMBL
Match: A0A6J1JZS3 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111488916 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.4e-64
Identity = 128/141 (90.78%), Postives = 133/141 (94.33%), Query Frame = 0


Query: 44  IMDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVS 103
           +MDK+ALYEKVFKQVDGDGDGKLS  ELQRCMA VGGSLTLEEAE  VENLD+DGDGL+ 
Sbjct: 1   MMDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMAVENLDTDGDGLMG 60

Query: 104 WAEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMI 163
           W EFVAF+EEVGEEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMI
Sbjct: 61  WGEFVAFVEEVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMI 120

Query: 164 AKFDLNSDGVLNFDEFKFMMS 185
           AKFDLNSDGVLNFDEFKFMMS
Sbjct: 121 AKFDLNSDGVLNFDEFKFMMS 141

[Close]

BLAST of CmaCh04G029650 vs. ExPASy TrEMBL
Match: A0A6J1BWC8 (putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC111006309 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.5e-63
Identity = 127/140 (90.71%), Postives = 130/140 (92.86%), Query Frame = 0


Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDK+ALYEKVF QVDGDGDGKLS PELQ+CMA VGGSLTLEEAE VV NLDSDGDGLV W
Sbjct: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
           AEFVAF+E V EEEKV DL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMMS 185
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of CmaCh04G029650 vs. ExPASy TrEMBL
Match: A0A6J1FET5 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111444836 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.0e-62
Identity = 126/141 (89.36%), Postives = 131/141 (92.91%), Query Frame = 0


Query: 44  IMDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVS 103
           +MDK+ALYEKVFKQVDGDGDGKLS  ELQRCMA VGGSLTLEEAE VVENLD+DGDGL+ 
Sbjct: 1   MMDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMG 60

Query: 104 WAEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMI 163
           W EFVAF+E VGEEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMI
Sbjct: 61  WGEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMI 120

Query: 164 AKFDLNSDGVLNFDEFKFMMS 185
           AKFDLNSDGVLNFDEF FMMS
Sbjct: 121 AKFDLNSDGVLNFDEFMFMMS 141

[Close]

BLAST of CmaCh04G029650 vs. NCBI nr
Match: KAG6578374.1 (Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 280.8 bits (717), Expect = 8.5e-72
Identity = 142/167 (85.03%), Postives = 149/167 (89.22%), Query Frame = 0


Query: 18   NGHPPAPRVDGNHHFHLPTSSPVKLRIMDKIALYEKVFKQVDGDGDGKLSLPELQRCMAV 77
            NG P A R+DGN HFH PT+  VK R+MDK+ALYEKVFKQVDGDGDGKLS  ELQRCMA 
Sbjct: 1282 NGRPSASRLDGNCHFHSPTNPAVKPRMMDKMALYEKVFKQVDGDGDGKLSPLELQRCMAG 1341

Query: 78   VGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGF 137
            VGGSLTLEEAE VVENLD+DGDGL+ W EFVAF+E VGEEEKVSDL EAFRMYEMDGCGF
Sbjct: 1342 VGGSLTLEEAEMVVENLDTDGDGLMGWGEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGF 1401

Query: 138  ITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS 185
            ITTKSLKRMLSRLGESRSIEEC KMIAKFDLNSDGVLNFDEF FMMS
Sbjct: 1402 ITTKSLKRMLSRLGESRSIEECNKMIAKFDLNSDGVLNFDEFMFMMS 1448

[Close]

BLAST of CmaCh04G029650 vs. NCBI nr
Match: XP_022991130.1 (putative calcium-binding protein CML19 [Cucurbita maxima] >XP_022991131.1 putative calcium-binding protein CML19 [Cucurbita maxima])

HSP 1 Score: 278.1 bits (710), Expect = 5.5e-71
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
           AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMMS 185
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of CmaCh04G029650 vs. NCBI nr
Match: XP_022955599.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 putative calcium-binding protein CML19 [Cucurbita moschata] >KAG6602674.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033361.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 275.4 bits (703), Expect = 3.6e-70
Identity = 139/140 (99.29%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
           AEFVAFME VGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMMS 185
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of CmaCh04G029650 vs. NCBI nr
Match: XP_023543960.1 (putative calcium-binding protein CML19 [Cucurbita pepo subsp. pepo] >XP_023543968.1 putative calcium-binding protein CML19 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 273.5 bits (698), Expect = 1.4e-69
Identity = 138/140 (98.57%), Postives = 138/140 (98.57%), Query Frame = 0


Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDKIALYEKVFKQVDGDGDG LSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGNLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
           AEFVAFME VGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMMS 185
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of CmaCh04G029650 vs. NCBI nr
Match: XP_022992628.1 (putative calcium-binding protein CML19 [Cucurbita maxima])

HSP 1 Score: 255.0 bits (650), Expect = 5.0e-64
Identity = 128/141 (90.78%), Postives = 133/141 (94.33%), Query Frame = 0


Query: 44  IMDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVS 103
           +MDK+ALYEKVFKQVDGDGDGKLS  ELQRCMA VGGSLTLEEAE  VENLD+DGDGL+ 
Sbjct: 1   MMDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMAVENLDTDGDGLMG 60

Query: 104 WAEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMI 163
           W EFVAF+EEVGEEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMI
Sbjct: 61  WGEFVAFVEEVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMI 120

Query: 164 AKFDLNSDGVLNFDEFKFMMS 185
           AKFDLNSDGVLNFDEFKFMMS
Sbjct: 121 AKFDLNSDGVLNFDEFKFMMS 141

[Close]

BLAST of CmaCh04G029650 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 128.3 bits (321), Expect = 6.6e-30
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = 0


Query: 54  VFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFM-- 113
           VF  +D + DGK+S  ELQ C++++GG+L+  E E+VV+  D DGDG + + EF+  M  
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 114 EEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 173
           E+  +EE+  +L EAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 174 GVLNFDEFKFMM 184
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of CmaCh04G029650 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 119.4 bits (298), Expect = 3.0e-27
Identity = 64/138 (46.38%), Postives = 89/138 (64.49%), Query Frame = 0


Query: 46  DKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWA 105
           DK    E VF  +D + DG++S  ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 106 EFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 165
           EF   ++   EEEK  +L  AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 166 FDLNSDGVLNFDEFKFMM 184
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of CmaCh04G029650 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 119.4 bits (298), Expect = 3.0e-27
Identity = 64/138 (46.38%), Postives = 89/138 (64.49%), Query Frame = 0


Query: 46  DKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWA 105
           DK    E VF  +D + DG++S  ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 106 EFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 165
           EF   ++   EEEK  +L  AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 166 FDLNSDGVLNFDEFKFMM 184
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of CmaCh04G029650 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 119.0 bits (297), Expect = 4.0e-27
Identity = 66/135 (48.89%), Postives = 87/135 (64.44%), Query Frame = 0


Query: 52  EKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFM 111
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V+  D DGDG++   EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 112 ---EEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 171
              +E  EEEK   +MEAFRMY  DG   IT  SLK ML +LGESR+ ++CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 172 NSDGVLNFDEFKFMM 184
           N+DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]

BLAST of CmaCh04G029650 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 112.1 bits (279), Expect = 4.9e-25
Identity = 59/138 (42.75%), Postives = 89/138 (64.49%), Query Frame = 0


Query: 52  EKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVA-F 111
           +KVF++ D +GDGK+S+ EL+  +  +  + + EE   +++  D DG+G +   EFVA F
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 112 MEEVG----EEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKF 171
              +G        VSDL EAF +Y++DG G I+ K L  ++  LGE  S+++CKKMI+K 
Sbjct: 79  QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKV 138

Query: 172 DLNSDGVLNFDEFKFMMS 185
           D++ DG +NFDEFK MMS
Sbjct: 139 DIDGDGCVNFDEFKKMMS 156

The following BLAST results are available for this feature:

BLAST of CmaCh04G029650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYJ8	2.2e-30	53.24	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FIH9	9.2e-29	50.76	Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1	[more]
Q8RYK0	6.0e-28	49.26	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE6	4.3e-26	46.38	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q9SRE7	5.6e-26	48.89	Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1	[more]

BLAST of CmaCh04G029650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JS06	2.7e-71	100.00	putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114878...	[more]
A0A6J1GWQ9	1.7e-70	99.29	putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1JZS3	2.4e-64	90.78	putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114889...	[more]
A0A6J1BWC8	3.5e-63	90.71	putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1FET5	1.0e-62	89.36	putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of CmaCh04G029650 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6578374.1	8.5e-72	85.03	Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022991130.1	5.5e-71	100.00	putative calcium-binding protein CML19 [Cucurbita maxima] >XP_022991131.1 putati...	[more]
XP_022955599.1	3.6e-70	99.29	putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 puta...	[more]
XP_023543960.1	1.4e-69	98.57	putative calcium-binding protein CML19 [Cucurbita pepo subsp. pepo] >XP_02354396...	[more]
XP_022992628.1	5.0e-64	90.78	putative calcium-binding protein CML19 [Cucurbita maxima]	[more]

BLAST of CmaCh04G029650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42380.1	6.6e-30	50.76	calmodulin like 37	[more]
AT1G76650.1	3.0e-27	46.38	calmodulin-like 38	[more]
AT1G76650.3	3.0e-27	46.38	calmodulin-like 38	[more]
AT1G76640.1	4.0e-27	48.89	Calcium-binding EF-hand family protein	[more]
AT5G37770.1	4.9e-25	42.75	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 158..184 e-value: 0.26 score: 20.3 coord: 86..114 e-value: 1.6 score: 16.0 coord: 122..150 e-value: 0.73 score: 18.9 coord: 50..78 e-value: 0.041 score: 23.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 50..112 e-value: 1.4E-13 score: 51.0 coord: 122..183 e-value: 5.9E-11 score: 42.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 82..117 score: 13.592857
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 118..153 score: 11.02648
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 154..184 score: 14.318138
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 46..81 score: 13.425486
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 122..184 e-value: 6.14047E-16 score: 66.8025
None	No IPR available	GENE3D	1.10.238.10		coord: 116..184 e-value: 4.7E-19 score: 70.4
None	No IPR available	GENE3D	1.10.238.10		coord: 32..115 e-value: 8.4E-18 score: 66.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR10891:SF858	EF HAND CALCIUM-BINDING FAMILY PROTEIN	coord: 46..184
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 46..184
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 167..179
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 59..71
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 95..107
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 48..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G029650.1	CmaCh04G029650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding