CmaCh04G029320 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G029320
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionrop guanine nucleotide exchange factor 5
LocationCma_Chr04: 19348709 .. 19351325 (+)
RNA-Seq ExpressionCmaCh04G029320
SyntenyCmaCh04G029320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGTAACCTTTAGGAGAGAAACAGAGAGAGAAATTTGAAATCCTTCCGCCATGTTTGAACCCGGTGAACCATCCAAGCTTCTTTAAAAAACCCACTGTGCTCACTCTTTTCCTTCCCTTTCGGGGTCTCGAAATCGCTCTTCTTTCCCAAAAATGGAACCTACTTCGGTCACAAGAAGCGAAAATACTCACAAAATCGGTGAATCTTTTGGTTCCAATGCTGTTGGAACCGAGTCGTTTACTGACTCCAGTGCTGTCTCAGGGGAGAACAGCTCAAGCAGGTCGAGCTCCGACAATTCCGCCGGCGACTTAAAGACCACCGACTGTTCTTCACCGCCTCAACTCGGCTGGCCCATCCGCAAGGCTCAGCTTCGCAGAAGTTCAAATTCCAAAGCTCGTGAAGTTGAACAAAAGCCCAATTTCAACGACTCAAAGCTCAACAAAACGGTTCCTGAAGTTCCTGAAGTTTCAGGTTAGTTTGATTTCATTCTCTGTTTAAAACCCATTTTGATTTTGGGGAGCTGATGAAGTTTTTGATGAACAGAGATGGAGATGATGAAGGAGAGATTTGCAAAACTGTTGCTTGGAGAAGACATGTCAGGCTCCGGTAAAGGGGTTTCCACTGCTTTGGCCATTTCAAACTCCATTACCAATCTCTGTGGTAAGCTTTTATCTATGAAACTTCCTTTCTTTTCTGCTTTAATGGCTATTTACACTGTTTTGTTCATCTTGAAGCTACCATTTTTGGGCAACTATGGAGACTCGAACCCTTGCCAAAGGAGAAGAAATCAATGTGGAGAAGAGAAATAGAGTGGCTTCTTTCTGTGAGTGACCATATAGTTGAATTAATCCCCTCTTTCCAAACATTCCCTGATGGAAGCAAGTTGGAGGTTATGACTTCCAGGCCAAGATCTGATATCTTCATCAATCTTCCAGCTCTCAGAAAATTGGACAACATGCTGCTTGTAAGATTACCCTTGTTTTTGTTGTTCATTACACTGTTCAATTCATGATATTCTTGGCCTTGTTTATAGGAAATTCTAGAGAGCTTCGTCGATACCAAGTTTTGGTATGTCGATCAAGGCATCATCGCTTCCAACGGCGATGGATCGTCGTCTTTTCGAAAAATAGTTCAAAGGCAACAGGAAAAGTGGTGGCTACCGGTGCCTCGAGTCCCGCCCGGCGGTCTTAGTGAAGATTCAAGAAAGCAATTGCATCATACTAGAGACTGCACAAACCAGATCTTGAAGGCTGCAATGGCTATCAATAATGTTGCCTTGGCTAACATGGAGGTGCCTGAATCATACTTGGAGACACTTCCTAAGGTTTGGATGGCAGTGTTTTTAAATTTTTGTGTAAAACATAATGCCTTGAAGAACTGCTTGACTTTTGAAACTTTGCTGCAGAATGGGAGAGCTTGTTTAGGGGATGTTATATACCGGTATATTACGTCGGAGCGTTTTTCATCTGAGAATTTGCTCGATTGCCTCGACTTATCCTCCGAACATGTTGCTCTCGATGTTGCCAATCGTGTCGAGGCTGCGATCTATGTCTGGCGGCGTCGAGCTCATTCGAAACCATTGATCAATCCAGCTCGTTCTACTGCAAGATCATCTTGGGAGATGGTGAAGGACCTAATGATTGATGGTGACAAGAGAGAGTTCTTAGCTAAAAGAGCTGAGGGCCTCTTGAATTCATTGAAGCAACGATTTCCTACACTAACACAAACCACTTTAGACACTAGCAAGATCCAATTCAACAAGGTACCAATGTTCACTCTTACACCTTGTTGTTGTTATCATTCATTTCATATTCATATCATGTAAAGCACATTTCAGGACGTTGGAAAGTCCATTCTTGAGAGCTACTCAAGAGTGCTAGAGAGTTTTGCATTCAACATCATTGCTCGAATCGACGACTTGCTTCACGTCGATGACTTAACTAAGCATTCGGATCGGTTGACATCAGTCGGGACGGTTAATGTAGTTGGTCATAAGAAGGGCGCTTACTCGGTGCATCTCTCGAGCACTCCATATAAACTCGCATCCACAACTCCATTGGTTAGTCCAGCCAAAACGGAGAGAGTGCCGTTTCTGAACAACAAGAACATAGGCAAGCCTCCTCGGCGCGGGTTCGGAGTGAAAAGAGTGTTGACGAATTATCTCGGCGTCGATACGAAAGCGAAAACGTGCATAGATTCGATTGAAGGGGCTGCTTCAATTCAGAACATTGTTGTGAATGGCAGTTCAGAACGGAGAAACAGTTCTTCTGCAGATCAAAGTGTGGTGAGCTGCAGCAAATTGCTGGCTGACAAGATCAAATAGTCAGTGCAGGAGGATAAAGAGGAAGAACATAGTAGTATACAGAAATAGAGTAGAATCAATTTGTTGTAAAAACTCTTTCGGTGAGAAATTATTTGTATTTATTTGATGATGCTTGAGTTCTGCTTTTTTAGAACCTAAAGCTGTTTTTTCTAGAATTGCTTGTATTGTTGTATTGTGCTATATTGTTCCTGACCAACTTTTTATTTATTTTATTTGTTTTGTTTTTTTCCGCTCACAAAAACTATCCCATCCCTTCCCACCCGAGCCTCACTCGATGGTCGAGTAGATATGTTG

mRNA sequence

CAGTAACCTTTAGGAGAGAAACAGAGAGAGAAATTTGAAATCCTTCCGCCATGTTTGAACCCGGTGAACCATCCAAGCTTCTTTAAAAAACCCACTGTGCTCACTCTTTTCCTTCCCTTTCGGGGTCTCGAAATCGCTCTTCTTTCCCAAAAATGGAACCTACTTCGGTCACAAGAAGCGAAAATACTCACAAAATCGGTGAATCTTTTGGTTCCAATGCTGTTGGAACCGAGTCGTTTACTGACTCCAGTGCTGTCTCAGGGGAGAACAGCTCAAGCAGGTCGAGCTCCGACAATTCCGCCGGCGACTTAAAGACCACCGACTGTTCTTCACCGCCTCAACTCGGCTGGCCCATCCGCAAGGCTCAGCTTCGCAGAAGTTCAAATTCCAAAGCTCGTGAAGTTGAACAAAAGCCCAATTTCAACGACTCAAAGCTCAACAAAACGGTTCCTGAAGTTCCTGAAGTTTCAGAGATGGAGATGATGAAGGAGAGATTTGCAAAACTGTTGCTTGGAGAAGACATGTCAGGCTCCGGTAAAGGGGTTTCCACTGCTTTGGCCATTTCAAACTCCATTACCAATCTCTGTGCTACCATTTTTGGGCAACTATGGAGACTCGAACCCTTGCCAAAGGAGAAGAAATCAATGTGGAGAAGAGAAATAGAGTGGCTTCTTTCTGTGAGTGACCATATAGTTGAATTAATCCCCTCTTTCCAAACATTCCCTGATGGAAGCAAGTTGGAGGTTATGACTTCCAGGCCAAGATCTGATATCTTCATCAATCTTCCAGCTCTCAGAAAATTGGACAACATGCTGCTTGAAATTCTAGAGAGCTTCGTCGATACCAAGTTTTGGTATGTCGATCAAGGCATCATCGCTTCCAACGGCGATGGATCGTCGTCTTTTCGAAAAATAGTTCAAAGGCAACAGGAAAAGTGGTGGCTACCGGTGCCTCGAGTCCCGCCCGGCGGTCTTAGTGAAGATTCAAGAAAGCAATTGCATCATACTAGAGACTGCACAAACCAGATCTTGAAGGCTGCAATGGCTATCAATAATGTTGCCTTGGCTAACATGGAGGTGCCTGAATCATACTTGGAGACACTTCCTAAGAATGGGAGAGCTTGTTTAGGGGATGTTATATACCGGTATATTACGTCGGAGCGTTTTTCATCTGAGAATTTGCTCGATTGCCTCGACTTATCCTCCGAACATGTTGCTCTCGATGTTGCCAATCGTGTCGAGGCTGCGATCTATGTCTGGCGGCGTCGAGCTCATTCGAAACCATTGATCAATCCAGCTCGTTCTACTGCAAGATCATCTTGGGAGATGGTGAAGGACCTAATGATTGATGGTGACAAGAGAGAGTTCTTAGCTAAAAGAGCTGAGGGCCTCTTGAATTCATTGAAGCAACGATTTCCTACACTAACACAAACCACTTTAGACACTAGCAAGATCCAATTCAACAAGGACGTTGGAAAGTCCATTCTTGAGAGCTACTCAAGAGTGCTAGAGAGTTTTGCATTCAACATCATTGCTCGAATCGACGACTTGCTTCACGTCGATGACTTAACTAAGCATTCGGATCGGTTGACATCAGTCGGGACGGTTAATGTAGTTGGTCATAAGAAGGGCGCTTACTCGGTGCATCTCTCGAGCACTCCATATAAACTCGCATCCACAACTCCATTGGTTAGTCCAGCCAAAACGGAGAGAGTGCCGTTTCTGAACAACAAGAACATAGGCAAGCCTCCTCGGCGCGGGTTCGGAGTGAAAAGAGTGTTGACGAATTATCTCGGCGTCGATACGAAAGCGAAAACGTGCATAGATTCGATTGAAGGGGCTGCTTCAATTCAGAACATTGTTGTGAATGGCAGTTCAGAACGGAGAAACAGTTCTTCTGCAGATCAAAGTGTGGTGAGCTGCAGCAAATTGCTGGCTGACAAGATCAAATAGTCAGTGCAGGAGGATAAAGAGGAAGAACATAGTAGTATACAGAAATAGAGTAGAATCAATTTGTTGTAAAAACTCTTTCGGTGAGAAATTATTTGTATTTATTTGATGATGCTTGAGTTCTGCTTTTTTAGAACCTAAAGCTGTTTTTTCTAGAATTGCTTGTATTGTTGTATTGTGCTATATTGTTCCTGACCAACTTTTTATTTATTTTATTTGTTTTGTTTTTTTCCGCTCACAAAAACTATCCCATCCCTTCCCACCCGAGCCTCACTCGATGGTCGAGTAGATATGTTG

Coding sequence (CDS)

ATGGAACCTACTTCGGTCACAAGAAGCGAAAATACTCACAAAATCGGTGAATCTTTTGGTTCCAATGCTGTTGGAACCGAGTCGTTTACTGACTCCAGTGCTGTCTCAGGGGAGAACAGCTCAAGCAGGTCGAGCTCCGACAATTCCGCCGGCGACTTAAAGACCACCGACTGTTCTTCACCGCCTCAACTCGGCTGGCCCATCCGCAAGGCTCAGCTTCGCAGAAGTTCAAATTCCAAAGCTCGTGAAGTTGAACAAAAGCCCAATTTCAACGACTCAAAGCTCAACAAAACGGTTCCTGAAGTTCCTGAAGTTTCAGAGATGGAGATGATGAAGGAGAGATTTGCAAAACTGTTGCTTGGAGAAGACATGTCAGGCTCCGGTAAAGGGGTTTCCACTGCTTTGGCCATTTCAAACTCCATTACCAATCTCTGTGCTACCATTTTTGGGCAACTATGGAGACTCGAACCCTTGCCAAAGGAGAAGAAATCAATGTGGAGAAGAGAAATAGAGTGGCTTCTTTCTGTGAGTGACCATATAGTTGAATTAATCCCCTCTTTCCAAACATTCCCTGATGGAAGCAAGTTGGAGGTTATGACTTCCAGGCCAAGATCTGATATCTTCATCAATCTTCCAGCTCTCAGAAAATTGGACAACATGCTGCTTGAAATTCTAGAGAGCTTCGTCGATACCAAGTTTTGGTATGTCGATCAAGGCATCATCGCTTCCAACGGCGATGGATCGTCGTCTTTTCGAAAAATAGTTCAAAGGCAACAGGAAAAGTGGTGGCTACCGGTGCCTCGAGTCCCGCCCGGCGGTCTTAGTGAAGATTCAAGAAAGCAATTGCATCATACTAGAGACTGCACAAACCAGATCTTGAAGGCTGCAATGGCTATCAATAATGTTGCCTTGGCTAACATGGAGGTGCCTGAATCATACTTGGAGACACTTCCTAAGAATGGGAGAGCTTGTTTAGGGGATGTTATATACCGGTATATTACGTCGGAGCGTTTTTCATCTGAGAATTTGCTCGATTGCCTCGACTTATCCTCCGAACATGTTGCTCTCGATGTTGCCAATCGTGTCGAGGCTGCGATCTATGTCTGGCGGCGTCGAGCTCATTCGAAACCATTGATCAATCCAGCTCGTTCTACTGCAAGATCATCTTGGGAGATGGTGAAGGACCTAATGATTGATGGTGACAAGAGAGAGTTCTTAGCTAAAAGAGCTGAGGGCCTCTTGAATTCATTGAAGCAACGATTTCCTACACTAACACAAACCACTTTAGACACTAGCAAGATCCAATTCAACAAGGACGTTGGAAAGTCCATTCTTGAGAGCTACTCAAGAGTGCTAGAGAGTTTTGCATTCAACATCATTGCTCGAATCGACGACTTGCTTCACGTCGATGACTTAACTAAGCATTCGGATCGGTTGACATCAGTCGGGACGGTTAATGTAGTTGGTCATAAGAAGGGCGCTTACTCGGTGCATCTCTCGAGCACTCCATATAAACTCGCATCCACAACTCCATTGGTTAGTCCAGCCAAAACGGAGAGAGTGCCGTTTCTGAACAACAAGAACATAGGCAAGCCTCCTCGGCGCGGGTTCGGAGTGAAAAGAGTGTTGACGAATTATCTCGGCGTCGATACGAAAGCGAAAACGTGCATAGATTCGATTGAAGGGGCTGCTTCAATTCAGAACATTGTTGTGAATGGCAGTTCAGAACGGAGAAACAGTTCTTCTGCAGATCAAAGTGTGGTGAGCTGCAGCAAATTGCTGGCTGACAAGATCAAATAG

Protein sequence

MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSSPPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGIIASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAINNVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGFGVKRVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK
Homology
BLAST of CmaCh04G029320 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 6.8e-182
Identity = 349/586 (59.56%), Postives = 430/586 (73.38%), Query Frame = 0

Query: 26  TESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSSPPQ----LGWPIRKAQLRRSSNSKA 85
           T S  D    S E SS+  +S  S+        S PP     LGWPIRKA  R++S    
Sbjct: 19  TPSMEDILTESKERSSTSGASYESSSTTTVASSSPPPPPSQILGWPIRKASFRKNSKESV 78

Query: 86  REVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLLGEDMSGSGKGVSTALAISNSI 145
               +K   +D    K   +    +++EMMKERFAKLLLGEDMSGSGKGV TALAISN+I
Sbjct: 79  NFDHKKLTLHDDSGFKA--KEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALAISNAI 138

Query: 146 TNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHIVELIPSFQTFPDGSKLEVMTS 205
           TNLCATIFGQLWRLEPL  EKK MWRRE+EW+LSVSDHIVEL PS QT+PDG+K EVMT 
Sbjct: 139 TNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTC 198

Query: 206 RPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGIIASNGDGSSSFRKIVQRQQEK 265
           RPR D+FINLPALRKLDNMLL+IL SF  T+FWYVDQGI+AS  DGS+SFR+ +QRQ+EK
Sbjct: 199 RPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRKIQRQEEK 258

Query: 266 WWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAINNVALANMEVPESYLETLPKNG 325
           WWLPVPR+ P GL+E++R +L+H R+C  QILKAAMAIN++AL  M+VP+SYLETLPKNG
Sbjct: 259 WWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNG 318

Query: 326 RACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPLI-- 385
           R+CLGDVIYRY+TS++FS+E+LLDCLDLSSEH+ALD+ANRVEA+IYVWRRR  +K  +  
Sbjct: 319 RSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNN 378

Query: 386 NPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKD 445
           N + +T + +WEMVK+LM  GDKR  L +R+E LL  LKQRFP+LTQT+LD SKIQ+NKD
Sbjct: 379 NTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWNKD 438

Query: 446 VGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD---RLTSVGTVNVVGHKKGA-- 505
           +GKSILESYSR LES A NIIARIDDLL+VDDLTK SD    L+S    +++ HKK    
Sbjct: 439 IGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVSSIIAHKKVVPL 498

Query: 506 -YSVHLSSTPYKLA-STTP-----LVSPAKTERVPFLNNKNIGK-----PPRRGFGVKRV 565
            Y +  S TPY+ + STTP     ++SP K ER    ++K+  K      P RG+GV+RV
Sbjct: 499 PYLISASGTPYRTSFSTTPGFSPSMISPKKGERRTPYSSKDTNKIIEKGLPSRGYGVRRV 558

Query: 566 LTNYLGVDTKAKTCIDSIEGA--ASIQNIVVNGSSE-RRNSSSADQ 586
           L NYLG+++K K C++  + A  A I  I  +   E +RNS+S  Q
Sbjct: 559 LNNYLGMESKLKICVNPSDNADTAVINQISKDVEEEKKRNSTSVHQ 602

BLAST of CmaCh04G029320 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 6.9e-166
Identity = 312/505 (61.78%), Postives = 375/505 (74.26%), Query Frame = 0

Query: 97  KTVPEVPEVSEMEMMKERFAKLLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLE 156
           K +     VSE+EMMKERF+KLLLGEDMSGSG GV TALAISN+ITNLCAT+FGQLWRLE
Sbjct: 60  KKIRSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLE 119

Query: 157 PLPKEKKSMWRREIEWLLSVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRK 216
           PLP EKK MWRRE+EWLL VSDHIVE+ P++QTFPDG+KLE+MT RPRSD+++NLPALRK
Sbjct: 120 PLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRK 179

Query: 217 LDNMLLEILESFVDTKFWYVDQGIIA--SNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGL 276
           LDNMLLEIL+SF +T+FWYVDQGI+A  S  DGSSSFRK  QRQ++KWWLPVPRV PGGL
Sbjct: 180 LDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGL 239

Query: 277 SEDSRKQLHHTRDCTNQILKAAMAINNVALANMEVPESYLETLPKNGRACLGDVIYRYIT 336
            E+SRKQL H RDCTNQILKAAMAIN++ LA+ME+PESYLE+LP+ GR+CLGD+IYRYI+
Sbjct: 240 QENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYIS 299

Query: 337 SERFSSENLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVK 396
           S++FS E LLDCLDLSSEH A+++ANRVE++IY+W +R +SKP  N      ++SWEMVK
Sbjct: 300 SDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATN-----TKTSWEMVK 359

Query: 397 DLMIDGDKREFLAKRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLES 456
           +LM+D DK E +A RAE LL SLKQRFP L QT LD SKIQ+NKD+GKSILESYSRVLES
Sbjct: 360 ELMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLES 419

Query: 457 FAFNIIARIDDLLHVDDLTKHSDRLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVS 516
            AFNI+ARIDDLL VDDLT+HS         N       + +V +S+       TTP  S
Sbjct: 420 LAFNIVARIDDLLFVDDLTRHSSDQIPTTLGNNGNDAPKSIAVPVSN------YTTPSYS 479

Query: 517 PAKTERVPFLNNKNIGKPPR-------RGFGVKRVLTNYLGVDTKAKTCIDSIEGAASIQ 576
           P+K E        +I  PP            VKRVLT Y+             +    ++
Sbjct: 480 PSKQEL-----RSSITVPPSPSRFKIPHSSSVKRVLTAYV------------TKNEPRLK 534

Query: 577 NIVVNGSSERRNSSSADQSVVSCSK 593
           N+ +  SS  R+SSS   S+  C K
Sbjct: 540 NLPLERSS--RSSSSERLSLEKCMK 534

BLAST of CmaCh04G029320 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 7.6e-149
Identity = 314/593 (52.95%), Postives = 399/593 (67.28%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSNAVG-----TESFTDSSAVSGENSSSRSSSDNSAGDLKT 60
           ME  S    E + + GE+     +G     TES TDSS  S   SSS   S +    +  
Sbjct: 1   MEDNSCIGFEGSKRFGET-SKRIIGLIDSVTESTTDSSLSS---SSSGVGSSSGRSSVAE 60

Query: 61  TDCSSPPQ----LGWPIRKAQLRRSSNSKAREVEQK-PNFNDSKLNKTVPEVPEVSEMEM 120
              SSPP     LGWP+ +   R+SS    ++   K  +F   ++        E SE+E+
Sbjct: 61  RSVSSPPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDFGFKRVG------TETSEIEL 120

Query: 121 MKERFAKLLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREI 180
           +KER AKLLLGEDMSGSG+GV  ALAISN+ITNL A I GQ WRLEP+P EKK MWRREI
Sbjct: 121 LKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREI 180

Query: 181 EWLLSVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVD 240
           E LLSVSDHIVEL+PSFQ FP+G+K+EVM  RPRSD+F  LPALRKLDNML+EIL+SF +
Sbjct: 181 EVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGE 240

Query: 241 TKFWYVDQGIIASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTN 300
           T+FWYVDQGI+A+    S+SFR+      +KWWLP+PRVP  GL+E +RK+L HTR+ TN
Sbjct: 241 TEFWYVDQGIVAAESARSNSFRE----DGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTN 300

Query: 301 QILKAAMAINNVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLS 360
           QILKA M+IN++ALA MEVP+SYLE LPKNGR+CLGD +YR ITS+ FS+++LL+ +DLS
Sbjct: 301 QILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLS 360

Query: 361 SEHVALDVANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMI---DGDKREFLA 420
           SE   +++ANRVEA++YVWRRRAHS+ LI+  RST+     +VK++M+   DGDKRE  A
Sbjct: 361 SELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTRWGMIVKEMMMHQTDGDKREIFA 420

Query: 421 KRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLL 480
           +RAE LL  LKQRFP L QT LDTSKIQ+NKDVGKSILESYSRVLES A++I  RI+++L
Sbjct: 421 ERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVL 480

Query: 481 HVDDLTKH---------SDRLTSV---------GTVNVVGHKKGAYSVHLS--STPYKLA 540
            +DD++K          SD+L  +         G++         +SV  S  STPY+  
Sbjct: 481 FMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTL 540

Query: 541 STT---------PLVSPAK----TERVPFLNNKNIGKPPRRGFGVKRVLTNYL 548
           S +         PL+SP       ER PFL+ +NI +  R GFG K+ L NYL
Sbjct: 541 SFSASTPSYSPMPLISPINGGRGGERAPFLSGRNIRE--RCGFGPKKALANYL 577

BLAST of CmaCh04G029320 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 480.7 bits (1236), Expect = 2.4e-134
Identity = 248/420 (59.05%), Postives = 318/420 (75.71%), Query Frame = 0

Query: 104 EVSEMEMMKERFAKLLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKK 163
           ++SE+EMMKERFAKLLLGEDMSG GKGV TALAISN+ITNL AT+FG+LWRLEPL  +KK
Sbjct: 87  DLSEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKK 146

Query: 164 SMWRREIEWLLSVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLE 223
           +MWRRE+EWLL VSD IVELIPS Q FP G   E+M +RPRSD++ NLPAL+KLD ML++
Sbjct: 147 AMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPALKKLDAMLID 206

Query: 224 ILESFVDTKFWYVDQGIIASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLH 283
           +L++F DT+FWY D+GI+  + D  S       RQ++KWWLP P+VPP GLSE++RK+L 
Sbjct: 207 MLDAFSDTEFWYTDRGIVLGDCDKDSYNSPASVRQEDKWWLPCPKVPPNGLSEEARKKLQ 266

Query: 284 HTRDCTNQILKAAMAINNVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENL 343
             RD  NQILKAA+AIN+  LA ME+P+ YLETLPK+G+ CLG++IY+Y+T+ +FS E L
Sbjct: 267 QCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYLTANKFSPECL 326

Query: 344 LDCLDLSSEHVALDVANRVEAAIYVWRR---RAHSKPLINPARSTARSSW-EMVKDLMID 403
           LDCLDLSSEH  L++ANR+EAA++VWR+   R H K           SSW   VK L+ D
Sbjct: 327 LDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKK-----QAKLKLSSWGGKVKGLVND 386

Query: 404 GDKREFLAKRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNI 463
            ++ +FL +RAE LL SL+ RFP L QTTLD +KIQ+NKDVG+SILESYSRV+ES AFNI
Sbjct: 387 NERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNI 446

Query: 464 IARIDDLLHVDDLTKHSDRLT---SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPA 517
            ARIDD+L+VDD  + S  +T   S+ ++N +  +K A+SV   S+P+     TP +S A
Sbjct: 447 TARIDDVLYVDDAMRRSISVTESLSLFSINGLNPQK-AFSV--QSSPHGSPFATPALSVA 498

BLAST of CmaCh04G029320 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 2.2e-116
Identity = 251/490 (51.22%), Postives = 327/490 (66.73%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSN---AVGTESFTD---SSAVSGENSSSRSSSDNSAGDLK 60
           ME  S     + H+   S   N   AV T  ++     S V     S  +S  +   D  
Sbjct: 1   MENLSNPDENDDHQSPRSIDQNDQSAVETPVYSTMSIDSFVYPRTCSETTSGFSDQIDET 60

Query: 61  TTDCSSPPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKER 120
            + CS      WP+    L  S +SK     +      S     V E+ E  E+E MKER
Sbjct: 61  NSFCSEASPCNWPV----LTESKSSKCLSGLEM----QSNECLVVQEISE-PELETMKER 120

Query: 121 FAKLLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLL 180
           FAKLLLGEDMSGSGKGV TA+ ISN+ITNL AT+FGQ  RLEPL  EK+++W+RE+  LL
Sbjct: 121 FAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALWKREMNCLL 180

Query: 181 SVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFW 240
           SV D+IVE IP  Q   +G+ +EVM SRPR+DI+INLPALRKLD+ML+E L+SF +T+FW
Sbjct: 181 SVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQNTEFW 240

Query: 241 YVDQGIIASNGDGSS--SFRK-IVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQ 300
           Y ++G ++     SS  SFRK IVQR++EKWWLPVP VP  GLS+ +RKQL + R+ TNQ
Sbjct: 241 YAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNKRESTNQ 300

Query: 301 ILKAAMAINNVALANMEVPESYLETLPKNGRACLGDVIYRYIT-SERFSSENLLDCLDLS 360
           I KAAMAIN+  L+ ME+P+SY+ TLPK G++ +GD IYRY++ S RF  E LLDCL++S
Sbjct: 301 IHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLLDCLNIS 360

Query: 361 SEHVALDVANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMID---GDKREFLA 420
           SEH A+ +A+RVEA++Y WRR++          S +++SW MVKDLM      DK   +A
Sbjct: 361 SEHEAVQLADRVEASMYTWRRKS--------CLSNSKNSWNMVKDLMSTTERTDKNYVMA 420

Query: 421 KRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLL 478
           +RAE LL  LKQR+P L+QT+LD  KIQ+NKDVGK++LESYSRVLE  AFNI+A IDD+L
Sbjct: 421 ERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNIVAWIDDVL 473

BLAST of CmaCh04G029320 vs. ExPASy TrEMBL
Match: A0A6J1JJ40 (rop guanine nucleotide exchange factor 5 OS=Cucurbita maxima OX=3661 GN=LOC111487404 PE=4 SV=1)

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 599/599 (100.00%), Postives = 599/599 (100.00%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60
           MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS
Sbjct: 1   MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60

Query: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLL 120
           PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLL
Sbjct: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLL 120

Query: 121 GEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHI 180
           GEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHI
Sbjct: 121 GEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHI 180

Query: 181 VELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGI 240
           VELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGI
Sbjct: 181 VELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGI 240

Query: 241 IASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAIN 300
           IASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAIN
Sbjct: 241 IASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAIN 300

Query: 301 NVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVAN 360
           NVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVAN
Sbjct: 301 NVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVAN 360

Query: 361 RVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQR 420
           RVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQR
Sbjct: 361 RVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQR 420

Query: 421 FPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLT 480
           FPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLT
Sbjct: 421 FPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLT 480

Query: 481 SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGFGVK 540
           SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGFGVK
Sbjct: 481 SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGFGVK 540

Query: 541 RVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK 600
           RVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK
Sbjct: 541 RVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK 599

BLAST of CmaCh04G029320 vs. ExPASy TrEMBL
Match: A0A6J1HHM7 (rop guanine nucleotide exchange factor 5 OS=Cucurbita moschata OX=3662 GN=LOC111463022 PE=4 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 585/602 (97.18%), Postives = 591/602 (98.17%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60
           MEP SVTRSENTHK+GESFGSN VGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS
Sbjct: 1   MEPASVTRSENTHKVGESFGSNVVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60

Query: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKT---VPEVPEVSEMEMMKERFAK 120
           PPQLGWPIRKAQLRRSSNSKAREVEQ PN NDSKLNKT   VPEVPEVSEMEMMKERFAK
Sbjct: 61  PPQLGWPIRKAQLRRSSNSKAREVEQNPNSNDSKLNKTVPEVPEVPEVSEMEMMKERFAK 120

Query: 121 LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVS 180
           LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKS+WRREIEWLLSVS
Sbjct: 121 LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSVWRREIEWLLSVS 180

Query: 181 DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVD 240
           DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDT+FWYVD
Sbjct: 181 DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVD 240

Query: 241 QGIIASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM 300
           QGIIAS+ DGSSSF+KIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM
Sbjct: 241 QGIIASDSDGSSSFQKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM 300

Query: 301 AINNVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD 360
           AINNVALA+MEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD
Sbjct: 301 AINNVALADMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD 360

Query: 361 VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL 420
           VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL
Sbjct: 361 VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL 420

Query: 421 KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD 480
           KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD
Sbjct: 421 KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD 480

Query: 481 RLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGF 540
           RLTSVGTVNVVGHKKGAYSVHLSSTPYKL STTPLVSPAKTERVPFLNNKNI KPPRRGF
Sbjct: 481 RLTSVGTVNVVGHKKGAYSVHLSSTPYKLTSTTPLVSPAKTERVPFLNNKNISKPPRRGF 540

Query: 541 GVKRVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADK 600
           GVKRVLTNYLGVDTKAKTCIDSIEGAASI NIVVNGSSERRNSSSADQSVVSCSKLLADK
Sbjct: 541 GVKRVLTNYLGVDTKAKTCIDSIEGAASIHNIVVNGSSERRNSSSADQSVVSCSKLLADK 600

BLAST of CmaCh04G029320 vs. ExPASy TrEMBL
Match: A0A6J1C216 (rop guanine nucleotide exchange factor 5 OS=Momordica charantia OX=3673 GN=LOC111006698 PE=4 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.9e-293
Identity = 528/602 (87.71%), Postives = 557/602 (92.52%), Query Frame = 0

Query: 5   SVTRSENTHKIGESFGSNAVGTE-SFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSSPPQ 64
           SV RSEN  KI  SFGSN VGTE S T+SSA SGE+SSSRSSSD S  D+K  +CSSPPQ
Sbjct: 4   SVKRSENAPKISGSFGSNGVGTESSLTESSAASGESSSSRSSSDTSGDDVKAGECSSPPQ 63

Query: 65  LGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLLGED 124
           LGWPIRKAQL +SSNSK REVEQKPN +DSKL+KT    P+VSEMEMMKERF+KLLLGED
Sbjct: 64  LGWPIRKAQLCKSSNSKVREVEQKPNSSDSKLSKT---APKVSEMEMMKERFSKLLLGED 123

Query: 125 MSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHIVEL 184
           MSGSGKGV TALAISN+ITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLL VSDHIVEL
Sbjct: 124 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLCVSDHIVEL 183

Query: 185 IPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGIIAS 244
           IPSFQTFPDGSKLEVMTSR RSDIFINLPALRKLDNMLLEILESFVDT+FWYVDQGI+AS
Sbjct: 184 IPSFQTFPDGSKLEVMTSRSRSDIFINLPALRKLDNMLLEILESFVDTEFWYVDQGIVAS 243

Query: 245 NGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAINNVA 304
           +GDGSSSFRKIVQRQQEKWWLPVPRVP GGL EDSRKQLHHTRDCTNQILKA +AINN+A
Sbjct: 244 DGDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVVAINNIA 303

Query: 305 LANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVANRVE 364
           LA+MEVPESYLETLPKNGRACLGDVIYRYITSE FSSE LLDCLDLSSEHVALDVANRVE
Sbjct: 304 LADMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVE 363

Query: 365 AAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQRFPT 424
           AAIYVWRRRAHSKP INP RSTARSSWEMVKDLMIDGDKREFLA+RAEGLL+SLKQRFP+
Sbjct: 364 AAIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPS 423

Query: 425 LTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLTSVG 484
           LTQTTLDTSKIQFNKDVGKSILESYSRVLES AFNIIARIDDLL+VDDLTKHSDRLTSVG
Sbjct: 424 LTQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSDRLTSVG 483

Query: 485 TVNVVGHKKGAYSVHLSSTPYKLASTT------PLVSPAKTERVPFLNNKNIGKPPRRGF 544
           TVNVVGHKK AYSVHLSSTPYKL +TT      PLVSPAK+ER+PFLNN+N+GKP RRGF
Sbjct: 484 TVNVVGHKKAAYSVHLSSTPYKLTATTPSFSPAPLVSPAKSERMPFLNNRNVGKPIRRGF 543

Query: 545 GVKRVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADK 600
           GVKR LTNYLGV+TKAKTC++SIEG ASIQNIVVN SSERR SSSADQSVVSCSKLL+DK
Sbjct: 544 GVKRALTNYLGVETKAKTCLNSIEGPASIQNIVVNESSERRKSSSADQSVVSCSKLLSDK 602

BLAST of CmaCh04G029320 vs. ExPASy TrEMBL
Match: A0A0A0K7N2 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G008860 PE=4 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.5e-285
Identity = 515/601 (85.69%), Postives = 548/601 (91.18%), Query Frame = 0

Query: 5   SVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSSPPQL 64
           SV  ++N  KI  SFGS   GTE FT+SSAVSG++SSSRSSSDNSAG     +CSSPPQL
Sbjct: 4   SVNTTDNARKIATSFGSIGAGTELFTESSAVSGQSSSSRSSSDNSAG-----ECSSPPQL 63

Query: 65  GWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLLGEDM 124
           GWPIRKAQL +SSNS+ REVEQKP+  DSK +KT   V +VSEME+MKERFAKLLLGEDM
Sbjct: 64  GWPIRKAQLCKSSNSRVREVEQKPSSTDSKPSKT---VTKVSEMELMKERFAKLLLGEDM 123

Query: 125 SGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHIVELI 184
           SGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMW+RE+EWLL VSDHIVELI
Sbjct: 124 SGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWKRELEWLLCVSDHIVELI 183

Query: 185 PSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGIIASN 244
           PSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DT+FWYVDQGI++S+
Sbjct: 184 PSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFADTEFWYVDQGIVSSD 243

Query: 245 GDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAINNVAL 304
           GDGSSSFRKIVQRQQEKWWLPVPRVP GGL EDSRKQLHHTRDCTNQILKA MAINN+AL
Sbjct: 244 GDGSSSFRKIVQRQQEKWWLPVPRVPAGGLGEDSRKQLHHTRDCTNQILKAVMAINNIAL 303

Query: 305 ANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVANRVEA 364
            +MEVPESYLETLPKNGRACLGDVIYRYITSE FSSE LLDCLDLSSEHVALDVANRVEA
Sbjct: 304 NDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVEA 363

Query: 365 AIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQRFPTL 424
           AIYVWRRRAHSKP INP RSTARSSWEMVKDLMIDGDKREFLA+RAEGLL+SLKQRFP+L
Sbjct: 364 AIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPSL 423

Query: 425 TQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLTSVGT 484
           TQTTLDTSKIQFNKDVGKSILESYSRVLES AFNIIARIDDLL+VDDLTKHS+RLTS  T
Sbjct: 424 TQTTLDTSKIQFNKDVGKSILESYSRVLESLAFNIIARIDDLLYVDDLTKHSNRLTSAAT 483

Query: 485 VNVVGHKKGAYSVHLSSTPYKLASTT------PLVSPAKTERVPFLNNKNIGKPPRRGFG 544
           VNVVGHKK AYSVHLSSTPYKL STT      PLVSPAKTERVPFLNNK   KPPRRGFG
Sbjct: 484 VNVVGHKKAAYSVHLSSTPYKLTSTTPSFSPAPLVSPAKTERVPFLNNKITSKPPRRGFG 543

Query: 545 VKRVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKI 600
           VKR LTNYLGV+TK KTC +SIEGAASIQNI+VN +SE+R SSSADQSV+SCSKLLA+K+
Sbjct: 544 VKRALTNYLGVETKPKTCTNSIEGAASIQNIIVNENSEQRKSSSADQSVISCSKLLAEKM 596

BLAST of CmaCh04G029320 vs. ExPASy TrEMBL
Match: A0A5D3DL37 (Rop guanine nucleotide exchange factor 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G001260 PE=4 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.6e-285
Identity = 512/601 (85.19%), Postives = 550/601 (91.51%), Query Frame = 0

Query: 5   SVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSSPPQL 64
           SV  ++N  KI  SFGS   GTE FT+SSAVSG++SSSRSSSDNSAG     +CSSPPQL
Sbjct: 4   SVKTTDNARKIAASFGSIGAGTELFTESSAVSGQSSSSRSSSDNSAG-----ECSSPPQL 63

Query: 65  GWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLLGEDM 124
           GWPIRKAQL +SSNS+ REVEQKP+ NDSK +KT   V +VSEME+MKERFAKLLLGEDM
Sbjct: 64  GWPIRKAQLCKSSNSRVREVEQKPSSNDSKPSKT---VTKVSEMELMKERFAKLLLGEDM 123

Query: 125 SGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHIVELI 184
           SGSGKGVSTALAISNSITNLCAT+FGQLWRLEPLPKEKKSMW+RE+EWLL VSDHIVELI
Sbjct: 124 SGSGKGVSTALAISNSITNLCATVFGQLWRLEPLPKEKKSMWKRELEWLLCVSDHIVELI 183

Query: 185 PSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGIIASN 244
           PSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESF DT+FWYVDQGI++S+
Sbjct: 184 PSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFADTEFWYVDQGIVSSD 243

Query: 245 GDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAINNVAL 304
           GDGSSSFRKIVQRQQEKWWLPVPRVP GGL+EDSRKQLHHTRDCTNQILKA MAINN+AL
Sbjct: 244 GDGSSSFRKIVQRQQEKWWLPVPRVPAGGLAEDSRKQLHHTRDCTNQILKAVMAINNIAL 303

Query: 305 ANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVANRVEA 364
            +MEVPESYLETLPKNGRACLGDVIYRYITSE FSSE LLDCLDLSSEHVALDVANRVEA
Sbjct: 304 NDMEVPESYLETLPKNGRACLGDVIYRYITSEHFSSEYLLDCLDLSSEHVALDVANRVEA 363

Query: 365 AIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQRFPTL 424
           AIYVWRRRAHSKP INP RSTARSSWEMVKDLMIDGDKREFLA+RAEGLL+SLKQRFP+L
Sbjct: 364 AIYVWRRRAHSKPQINPTRSTARSSWEMVKDLMIDGDKREFLAERAEGLLHSLKQRFPSL 423

Query: 425 TQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLTSVGT 484
           TQTTLDTSKIQFNKD+GKSILESYSRVLES A+NIIARIDDLL+VDDLTKHS+RLTS  T
Sbjct: 424 TQTTLDTSKIQFNKDIGKSILESYSRVLESLAYNIIARIDDLLYVDDLTKHSNRLTSAAT 483

Query: 485 VNVVGHKKGAYSVHLSSTPYKLASTT------PLVSPAKTERVPFLNNKNIGKPPRRGFG 544
           VNVVGHKK AYSVHLSSTPYKL STT      PLVSPAKTERVPFLNNK   KPPRRGFG
Sbjct: 484 VNVVGHKKAAYSVHLSSTPYKLTSTTPSFSPAPLVSPAKTERVPFLNNKITSKPPRRGFG 543

Query: 545 VKRVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKI 600
           VKR LTNYLGV+TK +TC +SIEGAASIQNI+VN +SE+R SSSADQSV+SCSKLLA+K+
Sbjct: 544 VKRALTNYLGVETKPRTCTNSIEGAASIQNIIVNENSEQRKSSSADQSVISCSKLLAEKM 596

BLAST of CmaCh04G029320 vs. NCBI nr
Match: XP_022990562.1 (rop guanine nucleotide exchange factor 5 [Cucurbita maxima])

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 599/599 (100.00%), Postives = 599/599 (100.00%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60
           MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS
Sbjct: 1   MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60

Query: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLL 120
           PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLL
Sbjct: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLL 120

Query: 121 GEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHI 180
           GEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHI
Sbjct: 121 GEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHI 180

Query: 181 VELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGI 240
           VELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGI
Sbjct: 181 VELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGI 240

Query: 241 IASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAIN 300
           IASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAIN
Sbjct: 241 IASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAIN 300

Query: 301 NVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVAN 360
           NVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVAN
Sbjct: 301 NVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVAN 360

Query: 361 RVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQR 420
           RVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQR
Sbjct: 361 RVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQR 420

Query: 421 FPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLT 480
           FPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLT
Sbjct: 421 FPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLT 480

Query: 481 SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGFGVK 540
           SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGFGVK
Sbjct: 481 SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGFGVK 540

Query: 541 RVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK 600
           RVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK
Sbjct: 541 RVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK 599

BLAST of CmaCh04G029320 vs. NCBI nr
Match: KAG7033326.1 (Rop guanine nucleotide exchange factor 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 587/602 (97.51%), Postives = 592/602 (98.34%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60
           MEP SVTRSENTHK+GESFGSN VGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS
Sbjct: 1   MEPASVTRSENTHKVGESFGSNVVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60

Query: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKT---VPEVPEVSEMEMMKERFAK 120
           PPQLGWPIRKAQLRRSSNSKAREVEQKPN NDSKLNKT   VPEVPEVSEMEMMKERFAK
Sbjct: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNSNDSKLNKTVPEVPEVPEVSEMEMMKERFAK 120

Query: 121 LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVS 180
           LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKS+WRREIEWLLSVS
Sbjct: 121 LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSVWRREIEWLLSVS 180

Query: 181 DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVD 240
           DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDT+FWYVD
Sbjct: 181 DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVD 240

Query: 241 QGIIASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM 300
           QGIIAS+ DGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM
Sbjct: 241 QGIIASDSDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM 300

Query: 301 AINNVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD 360
           AINNVALA+MEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD
Sbjct: 301 AINNVALADMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD 360

Query: 361 VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL 420
           VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL
Sbjct: 361 VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL 420

Query: 421 KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD 480
           KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD
Sbjct: 421 KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD 480

Query: 481 RLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGF 540
           RLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNI KPPRRGF
Sbjct: 481 RLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNISKPPRRGF 540

Query: 541 GVKRVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADK 600
           GVKRVLTNYLGVDTK KTCIDSIEGAASI NIVVNGSSERRNSSSADQSVVSCSKLLADK
Sbjct: 541 GVKRVLTNYLGVDTKVKTCIDSIEGAASIHNIVVNGSSERRNSSSADQSVVSCSKLLADK 600

BLAST of CmaCh04G029320 vs. NCBI nr
Match: KAG6602639.1 (Rop guanine nucleotide exchange factor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 586/602 (97.34%), Postives = 591/602 (98.17%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60
           MEP SVTRSENTHK+GESFGSN VGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS
Sbjct: 1   MEPASVTRSENTHKVGESFGSNVVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60

Query: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKT---VPEVPEVSEMEMMKERFAK 120
           PPQLGWPIRKAQLR SSNSKAREVEQKPN NDSKLNKT   VPEVPEVSEMEMMKERFAK
Sbjct: 61  PPQLGWPIRKAQLRISSNSKAREVEQKPNSNDSKLNKTVPEVPEVPEVSEMEMMKERFAK 120

Query: 121 LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVS 180
           LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKS+WRREIEWLLSVS
Sbjct: 121 LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSVWRREIEWLLSVS 180

Query: 181 DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVD 240
           DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDT+FWYVD
Sbjct: 181 DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVD 240

Query: 241 QGIIASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM 300
           QGIIAS+ DGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM
Sbjct: 241 QGIIASDSDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM 300

Query: 301 AINNVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD 360
           AINNVALA+MEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD
Sbjct: 301 AINNVALADMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD 360

Query: 361 VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL 420
           VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL
Sbjct: 361 VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL 420

Query: 421 KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD 480
           KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD
Sbjct: 421 KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD 480

Query: 481 RLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGF 540
           RLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNI KPPRRGF
Sbjct: 481 RLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNISKPPRRGF 540

Query: 541 GVKRVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADK 600
           GVKRVLTNYLGVDTK KTCIDSIEGAASI NIVVNGSSERRNSSSADQSVVSCSKLLADK
Sbjct: 541 GVKRVLTNYLGVDTKVKTCIDSIEGAASIHNIVVNGSSERRNSSSADQSVVSCSKLLADK 600

BLAST of CmaCh04G029320 vs. NCBI nr
Match: XP_022962629.1 (rop guanine nucleotide exchange factor 5 [Cucurbita moschata])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 585/602 (97.18%), Postives = 591/602 (98.17%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60
           MEP SVTRSENTHK+GESFGSN VGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS
Sbjct: 1   MEPASVTRSENTHKVGESFGSNVVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60

Query: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKT---VPEVPEVSEMEMMKERFAK 120
           PPQLGWPIRKAQLRRSSNSKAREVEQ PN NDSKLNKT   VPEVPEVSEMEMMKERFAK
Sbjct: 61  PPQLGWPIRKAQLRRSSNSKAREVEQNPNSNDSKLNKTVPEVPEVPEVSEMEMMKERFAK 120

Query: 121 LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVS 180
           LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKS+WRREIEWLLSVS
Sbjct: 121 LLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSVWRREIEWLLSVS 180

Query: 181 DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVD 240
           DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDT+FWYVD
Sbjct: 181 DHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTEFWYVD 240

Query: 241 QGIIASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM 300
           QGIIAS+ DGSSSF+KIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM
Sbjct: 241 QGIIASDSDGSSSFQKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAM 300

Query: 301 AINNVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD 360
           AINNVALA+MEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD
Sbjct: 301 AINNVALADMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALD 360

Query: 361 VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL 420
           VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL
Sbjct: 361 VANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSL 420

Query: 421 KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD 480
           KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD
Sbjct: 421 KQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD 480

Query: 481 RLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGF 540
           RLTSVGTVNVVGHKKGAYSVHLSSTPYKL STTPLVSPAKTERVPFLNNKNI KPPRRGF
Sbjct: 481 RLTSVGTVNVVGHKKGAYSVHLSSTPYKLTSTTPLVSPAKTERVPFLNNKNISKPPRRGF 540

Query: 541 GVKRVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADK 600
           GVKRVLTNYLGVDTKAKTCIDSIEGAASI NIVVNGSSERRNSSSADQSVVSCSKLLADK
Sbjct: 541 GVKRVLTNYLGVDTKAKTCIDSIEGAASIHNIVVNGSSERRNSSSADQSVVSCSKLLADK 600

BLAST of CmaCh04G029320 vs. NCBI nr
Match: XP_023515241.1 (rop guanine nucleotide exchange factor 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 582/599 (97.16%), Postives = 587/599 (98.00%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSNAVGTESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSS 60
           MEP SVTRSEN HKIGESFGSNAVGTESFTDSSAVSGEN SSRSSSDNSAGDLKTTDCSS
Sbjct: 1   MEPASVTRSENAHKIGESFGSNAVGTESFTDSSAVSGENGSSRSSSDNSAGDLKTTDCSS 60

Query: 61  PPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLL 120
           PPQLGWPIRKAQLRRSSNSKAREVEQ PN NDSKLNKT   VPEVSEMEMMKERFAKLLL
Sbjct: 61  PPQLGWPIRKAQLRRSSNSKAREVEQNPNSNDSKLNKT---VPEVSEMEMMKERFAKLLL 120

Query: 121 GEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHI 180
           GEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPK+KKSMWRREIEWLLSVSDHI
Sbjct: 121 GEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKDKKSMWRREIEWLLSVSDHI 180

Query: 181 VELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGI 240
           VELIPSFQTFPDGSKLEVMTSRPRSDIF+NLPALRKLDNMLLEILESFVDT+FWYVDQGI
Sbjct: 181 VELIPSFQTFPDGSKLEVMTSRPRSDIFMNLPALRKLDNMLLEILESFVDTEFWYVDQGI 240

Query: 241 IASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAIN 300
           IAS+GDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAIN
Sbjct: 241 IASDGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAIN 300

Query: 301 NVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVAN 360
           NVALA+MEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVAN
Sbjct: 301 NVALADMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVAN 360

Query: 361 RVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQR 420
           RVEAAIYVWRRRAHSKPLINPARSTARSSW MVKDLMIDGDKREFLAKRAE LLNSLKQR
Sbjct: 361 RVEAAIYVWRRRAHSKPLINPARSTARSSWAMVKDLMIDGDKREFLAKRAESLLNSLKQR 420

Query: 421 FPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLT 480
           FPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLT
Sbjct: 421 FPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSDRLT 480

Query: 481 SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNIGKPPRRGFGVK 540
           SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNI K PRRGFGVK
Sbjct: 481 SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPAKTERVPFLNNKNISKAPRRGFGVK 540

Query: 541 RVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK 600
           RVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK
Sbjct: 541 RVLTNYLGVDTKAKTCIDSIEGAASIQNIVVNGSSERRNSSSADQSVVSCSKLLADKIK 596

BLAST of CmaCh04G029320 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 638.6 bits (1646), Expect = 4.9e-183
Identity = 349/586 (59.56%), Postives = 430/586 (73.38%), Query Frame = 0

Query: 26  TESFTDSSAVSGENSSSRSSSDNSAGDLKTTDCSSPPQ----LGWPIRKAQLRRSSNSKA 85
           T S  D    S E SS+  +S  S+        S PP     LGWPIRKA  R++S    
Sbjct: 19  TPSMEDILTESKERSSTSGASYESSSTTTVASSSPPPPPSQILGWPIRKASFRKNSKESV 78

Query: 86  REVEQKPNFNDSKLNKTVPEVPEVSEMEMMKERFAKLLLGEDMSGSGKGVSTALAISNSI 145
               +K   +D    K   +    +++EMMKERFAKLLLGEDMSGSGKGV TALAISN+I
Sbjct: 79  NFDHKKLTLHDDSGFKA--KEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALAISNAI 138

Query: 146 TNLCATIFGQLWRLEPLPKEKKSMWRREIEWLLSVSDHIVELIPSFQTFPDGSKLEVMTS 205
           TNLCATIFGQLWRLEPL  EKK MWRRE+EW+LSVSDHIVEL PS QT+PDG+K EVMT 
Sbjct: 139 TNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTC 198

Query: 206 RPRSDIFINLPALRKLDNMLLEILESFVDTKFWYVDQGIIASNGDGSSSFRKIVQRQQEK 265
           RPR D+FINLPALRKLDNMLL+IL SF  T+FWYVDQGI+AS  DGS+SFR+ +QRQ+EK
Sbjct: 199 RPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRKIQRQEEK 258

Query: 266 WWLPVPRVPPGGLSEDSRKQLHHTRDCTNQILKAAMAINNVALANMEVPESYLETLPKNG 325
           WWLPVPR+ P GL+E++R +L+H R+C  QILKAAMAIN++AL  M+VP+SYLETLPKNG
Sbjct: 259 WWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNG 318

Query: 326 RACLGDVIYRYITSERFSSENLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPLI-- 385
           R+CLGDVIYRY+TS++FS+E+LLDCLDLSSEH+ALD+ANRVEA+IYVWRRR  +K  +  
Sbjct: 319 RSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNN 378

Query: 386 NPARSTARSSWEMVKDLMIDGDKREFLAKRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKD 445
           N + +T + +WEMVK+LM  GDKR  L +R+E LL  LKQRFP+LTQT+LD SKIQ+NKD
Sbjct: 379 NTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWNKD 438

Query: 446 VGKSILESYSRVLESFAFNIIARIDDLLHVDDLTKHSD---RLTSVGTVNVVGHKKGA-- 505
           +GKSILESYSR LES A NIIARIDDLL+VDDLTK SD    L+S    +++ HKK    
Sbjct: 439 IGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVSSIIAHKKVVPL 498

Query: 506 -YSVHLSSTPYKLA-STTP-----LVSPAKTERVPFLNNKNIGK-----PPRRGFGVKRV 565
            Y +  S TPY+ + STTP     ++SP K ER    ++K+  K      P RG+GV+RV
Sbjct: 499 PYLISASGTPYRTSFSTTPGFSPSMISPKKGERRTPYSSKDTNKIIEKGLPSRGYGVRRV 558

Query: 566 LTNYLGVDTKAKTCIDSIEGA--ASIQNIVVNGSSE-RRNSSSADQ 586
           L NYLG+++K K C++  + A  A I  I  +   E +RNS+S  Q
Sbjct: 559 LNNYLGMESKLKICVNPSDNADTAVINQISKDVEEEKKRNSTSVHQ 602

BLAST of CmaCh04G029320 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 585.5 bits (1508), Expect = 4.9e-167
Identity = 312/505 (61.78%), Postives = 375/505 (74.26%), Query Frame = 0

Query: 97  KTVPEVPEVSEMEMMKERFAKLLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLE 156
           K +     VSE+EMMKERF+KLLLGEDMSGSG GV TALAISN+ITNLCAT+FGQLWRLE
Sbjct: 60  KKIRSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLE 119

Query: 157 PLPKEKKSMWRREIEWLLSVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRK 216
           PLP EKK MWRRE+EWLL VSDHIVE+ P++QTFPDG+KLE+MT RPRSD+++NLPALRK
Sbjct: 120 PLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRK 179

Query: 217 LDNMLLEILESFVDTKFWYVDQGIIA--SNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGL 276
           LDNMLLEIL+SF +T+FWYVDQGI+A  S  DGSSSFRK  QRQ++KWWLPVPRV PGGL
Sbjct: 180 LDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGL 239

Query: 277 SEDSRKQLHHTRDCTNQILKAAMAINNVALANMEVPESYLETLPKNGRACLGDVIYRYIT 336
            E+SRKQL H RDCTNQILKAAMAIN++ LA+ME+PESYLE+LP+ GR+CLGD+IYRYI+
Sbjct: 240 QENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYIS 299

Query: 337 SERFSSENLLDCLDLSSEHVALDVANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVK 396
           S++FS E LLDCLDLSSEH A+++ANRVE++IY+W +R +SKP  N      ++SWEMVK
Sbjct: 300 SDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATN-----TKTSWEMVK 359

Query: 397 DLMIDGDKREFLAKRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLES 456
           +LM+D DK E +A RAE LL SLKQRFP L QT LD SKIQ+NKD+GKSILESYSRVLES
Sbjct: 360 ELMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLES 419

Query: 457 FAFNIIARIDDLLHVDDLTKHSDRLTSVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVS 516
            AFNI+ARIDDLL VDDLT+HS         N       + +V +S+       TTP  S
Sbjct: 420 LAFNIVARIDDLLFVDDLTRHSSDQIPTTLGNNGNDAPKSIAVPVSN------YTTPSYS 479

Query: 517 PAKTERVPFLNNKNIGKPPR-------RGFGVKRVLTNYLGVDTKAKTCIDSIEGAASIQ 576
           P+K E        +I  PP            VKRVLT Y+             +    ++
Sbjct: 480 PSKQEL-----RSSITVPPSPSRFKIPHSSSVKRVLTAYV------------TKNEPRLK 534

Query: 577 NIVVNGSSERRNSSSADQSVVSCSK 593
           N+ +  SS  R+SSS   S+  C K
Sbjct: 540 NLPLERSS--RSSSSERLSLEKCMK 534

BLAST of CmaCh04G029320 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 528.9 bits (1361), Expect = 5.4e-150
Identity = 314/593 (52.95%), Postives = 399/593 (67.28%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSNAVG-----TESFTDSSAVSGENSSSRSSSDNSAGDLKT 60
           ME  S    E + + GE+     +G     TES TDSS  S   SSS   S +    +  
Sbjct: 1   MEDNSCIGFEGSKRFGET-SKRIIGLIDSVTESTTDSSLSS---SSSGVGSSSGRSSVAE 60

Query: 61  TDCSSPPQ----LGWPIRKAQLRRSSNSKAREVEQK-PNFNDSKLNKTVPEVPEVSEMEM 120
              SSPP     LGWP+ +   R+SS    ++   K  +F   ++        E SE+E+
Sbjct: 61  RSVSSPPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDFGFKRVG------TETSEIEL 120

Query: 121 MKERFAKLLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREI 180
           +KER AKLLLGEDMSGSG+GV  ALAISN+ITNL A I GQ WRLEP+P EKK MWRREI
Sbjct: 121 LKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREI 180

Query: 181 EWLLSVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVD 240
           E LLSVSDHIVEL+PSFQ FP+G+K+EVM  RPRSD+F  LPALRKLDNML+EIL+SF +
Sbjct: 181 EVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGE 240

Query: 241 TKFWYVDQGIIASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTN 300
           T+FWYVDQGI+A+    S+SFR+      +KWWLP+PRVP  GL+E +RK+L HTR+ TN
Sbjct: 241 TEFWYVDQGIVAAESARSNSFRE----DGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTN 300

Query: 301 QILKAAMAINNVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENLLDCLDLS 360
           QILKA M+IN++ALA MEVP+SYLE LPKNGR+CLGD +YR ITS+ FS+++LL+ +DLS
Sbjct: 301 QILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLS 360

Query: 361 SEHVALDVANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMI---DGDKREFLA 420
           SE   +++ANRVEA++YVWRRRAHS+ LI+  RST+     +VK++M+   DGDKRE  A
Sbjct: 361 SELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTRWGMIVKEMMMHQTDGDKREIFA 420

Query: 421 KRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLL 480
           +RAE LL  LKQRFP L QT LDTSKIQ+NKDVGKSILESYSRVLES A++I  RI+++L
Sbjct: 421 ERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVL 480

Query: 481 HVDDLTKH---------SDRLTSV---------GTVNVVGHKKGAYSVHLS--STPYKLA 540
            +DD++K          SD+L  +         G++         +SV  S  STPY+  
Sbjct: 481 FMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTL 540

Query: 541 STT---------PLVSPAK----TERVPFLNNKNIGKPPRRGFGVKRVLTNYL 548
           S +         PL+SP       ER PFL+ +NI +  R GFG K+ L NYL
Sbjct: 541 SFSASTPSYSPMPLISPINGGRGGERAPFLSGRNIRE--RCGFGPKKALANYL 577

BLAST of CmaCh04G029320 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 480.7 bits (1236), Expect = 1.7e-135
Identity = 248/420 (59.05%), Postives = 318/420 (75.71%), Query Frame = 0

Query: 104 EVSEMEMMKERFAKLLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKK 163
           ++SE+EMMKERFAKLLLGEDMSG GKGV TALAISN+ITNL AT+FG+LWRLEPL  +KK
Sbjct: 87  DLSEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKK 146

Query: 164 SMWRREIEWLLSVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLE 223
           +MWRRE+EWLL VSD IVELIPS Q FP G   E+M +RPRSD++ NLPAL+KLD ML++
Sbjct: 147 AMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANLPALKKLDAMLID 206

Query: 224 ILESFVDTKFWYVDQGIIASNGDGSSSFRKIVQRQQEKWWLPVPRVPPGGLSEDSRKQLH 283
           +L++F DT+FWY D+GI+  + D  S       RQ++KWWLP P+VPP GLSE++RK+L 
Sbjct: 207 MLDAFSDTEFWYTDRGIVLGDCDKDSYNSPASVRQEDKWWLPCPKVPPNGLSEEARKKLQ 266

Query: 284 HTRDCTNQILKAAMAINNVALANMEVPESYLETLPKNGRACLGDVIYRYITSERFSSENL 343
             RD  NQILKAA+AIN+  LA ME+P+ YLETLPK+G+ CLG++IY+Y+T+ +FS E L
Sbjct: 267 QCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIYQYLTANKFSPECL 326

Query: 344 LDCLDLSSEHVALDVANRVEAAIYVWRR---RAHSKPLINPARSTARSSW-EMVKDLMID 403
           LDCLDLSSEH  L++ANR+EAA++VWR+   R H K           SSW   VK L+ D
Sbjct: 327 LDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKK-----QAKLKLSSWGGKVKGLVND 386

Query: 404 GDKREFLAKRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNI 463
            ++ +FL +RAE LL SL+ RFP L QTTLD +KIQ+NKDVG+SILESYSRV+ES AFNI
Sbjct: 387 NERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRVMESMAFNI 446

Query: 464 IARIDDLLHVDDLTKHSDRLT---SVGTVNVVGHKKGAYSVHLSSTPYKLASTTPLVSPA 517
            ARIDD+L+VDD  + S  +T   S+ ++N +  +K A+SV   S+P+     TP +S A
Sbjct: 447 TARIDDVLYVDDAMRRSISVTESLSLFSINGLNPQK-AFSV--QSSPHGSPFATPALSVA 498

BLAST of CmaCh04G029320 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 421.0 bits (1081), Expect = 1.6e-117
Identity = 251/490 (51.22%), Postives = 327/490 (66.73%), Query Frame = 0

Query: 1   MEPTSVTRSENTHKIGESFGSN---AVGTESFTD---SSAVSGENSSSRSSSDNSAGDLK 60
           ME  S     + H+   S   N   AV T  ++     S V     S  +S  +   D  
Sbjct: 1   MENLSNPDENDDHQSPRSIDQNDQSAVETPVYSTMSIDSFVYPRTCSETTSGFSDQIDET 60

Query: 61  TTDCSSPPQLGWPIRKAQLRRSSNSKAREVEQKPNFNDSKLNKTVPEVPEVSEMEMMKER 120
            + CS      WP+    L  S +SK     +      S     V E+ E  E+E MKER
Sbjct: 61  NSFCSEASPCNWPV----LTESKSSKCLSGLEM----QSNECLVVQEISE-PELETMKER 120

Query: 121 FAKLLLGEDMSGSGKGVSTALAISNSITNLCATIFGQLWRLEPLPKEKKSMWRREIEWLL 180
           FAKLLLGEDMSGSGKGV TA+ ISN+ITNL AT+FGQ  RLEPL  EK+++W+RE+  LL
Sbjct: 121 FAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALWKREMNCLL 180

Query: 181 SVSDHIVELIPSFQTFPDGSKLEVMTSRPRSDIFINLPALRKLDNMLLEILESFVDTKFW 240
           SV D+IVE IP  Q   +G+ +EVM SRPR+DI+INLPALRKLD+ML+E L+SF +T+FW
Sbjct: 181 SVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQNTEFW 240

Query: 241 YVDQGIIASNGDGSS--SFRK-IVQRQQEKWWLPVPRVPPGGLSEDSRKQLHHTRDCTNQ 300
           Y ++G ++     SS  SFRK IVQR++EKWWLPVP VP  GLS+ +RKQL + R+ TNQ
Sbjct: 241 YAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNKRESTNQ 300

Query: 301 ILKAAMAINNVALANMEVPESYLETLPKNGRACLGDVIYRYIT-SERFSSENLLDCLDLS 360
           I KAAMAIN+  L+ ME+P+SY+ TLPK G++ +GD IYRY++ S RF  E LLDCL++S
Sbjct: 301 IHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLLDCLNIS 360

Query: 361 SEHVALDVANRVEAAIYVWRRRAHSKPLINPARSTARSSWEMVKDLMID---GDKREFLA 420
           SEH A+ +A+RVEA++Y WRR++          S +++SW MVKDLM      DK   +A
Sbjct: 361 SEHEAVQLADRVEASMYTWRRKS--------CLSNSKNSWNMVKDLMSTTERTDKNYVMA 420

Query: 421 KRAEGLLNSLKQRFPTLTQTTLDTSKIQFNKDVGKSILESYSRVLESFAFNIIARIDDLL 478
           +RAE LL  LKQR+P L+QT+LD  KIQ+NKDVGK++LESYSRVLE  AFNI+A IDD+L
Sbjct: 421 ERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNIVAWIDDVL 473

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4K2956.8e-18259.56Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9LZN06.9e-16661.78Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9M0567.6e-14952.95Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q93ZY22.4e-13459.05Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
A4IJ272.2e-11651.22Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Match NameE-valueIdentityDescription
A0A6J1JJ400.0e+00100.00rop guanine nucleotide exchange factor 5 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1HHM70.0e+0097.18rop guanine nucleotide exchange factor 5 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1C2169.9e-29387.71rop guanine nucleotide exchange factor 5 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A0A0K7N21.5e-28585.69PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G008860 PE=4 ... [more]
A0A5D3DL372.6e-28585.19Rop guanine nucleotide exchange factor 5 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
Match NameE-valueIdentityDescription
XP_022990562.10.0e+00100.00rop guanine nucleotide exchange factor 5 [Cucurbita maxima][more]
KAG7033326.10.0e+0097.51Rop guanine nucleotide exchange factor 5, partial [Cucurbita argyrosperma subsp.... [more]
KAG6602639.10.0e+0097.34Rop guanine nucleotide exchange factor 5, partial [Cucurbita argyrosperma subsp.... [more]
XP_022962629.10.0e+0097.18rop guanine nucleotide exchange factor 5 [Cucurbita moschata][more]
XP_023515241.10.0e+0097.16rop guanine nucleotide exchange factor 5-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G05940.14.9e-18359.56ROP guanine nucleotide exchange factor 5 [more]
AT5G02010.14.9e-16761.78RHO guanyl-nucleotide exchange factor 7 [more]
AT3G55660.15.4e-15052.95ROP (rho of plants) guanine nucleotide exchange factor 6 [more]
AT4G38430.11.7e-13559.05rho guanyl-nucleotide exchange factor 1 [more]
AT4G00460.21.6e-11751.22RHO guanyl-nucleotide exchange factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005512PRONE domainPFAMPF03759PRONEcoord: 106..474
e-value: 6.6E-184
score: 610.8
IPR005512PRONE domainPROSITEPS51334PRONEcoord: 98..480
score: 120.889221
NoneNo IPR availableGENE3D1.20.58.2010PRONE domain, subdomain 1coord: 342..480
e-value: 5.7E-18
score: 67.0
coord: 101..341
e-value: 5.7E-108
score: 361.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..96
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..90
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..62
NoneNo IPR availablePANTHERPTHR33101:SF1ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 5coord: 30..564
IPR038937Rop guanine nucleotide exchange factorPANTHERPTHR33101ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1coord: 30..564

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G029320.1CmaCh04G029320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity
molecular_function GO:0008289 lipid binding