CmaCh04G028490 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G028490
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein NETWORKED 4A-like
LocationCma_Chr04: 19014842 .. 19016588 (-)
RNA-Seq ExpressionCmaCh04G028490
SyntenyCmaCh04G028490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTCGGAATGTTGAGCAGATGCTGAAGGCGATGAAAGAAAATAGTGATAATTTTCCGAAAAGTGCTGATATGGATTCTCAAGTTGAGGAGTTCTCTCGCTTGTATCAATCGTTGGTTGAAAACGTGTTATCGCCGGAGCTACAATTGCAGGTACCTGTTTATTCTGATTGTGGATCTCCTCGAGGCACTCCGGAATTAAGTTTCAACCAAAAACAGGATTTCAATATGTCCAGCAATAGAGGCTTGGATGTCTCTTTTGATTCAGGTGGTGGTAGCTCCAGCCTTTCTCTGAAGGACGGAAGTGAATCTTCTTCTTCTTCATCGGACTCTGAATTAGAATCTGTCGACAATCACTATGCGGTTTCACGTGATCAAAGAGATGGTCAAGGGCAGAAGAAAAAACTTCTTGAGCTACAAACTGAGCTTCCTCCTAAAATTAGAGGGGCATTATGGGGGGATGAGGAAGACAAGGTAAATTATGATGAGTTGTACGAAAGAATCACCAGGCTTGAGGAAGAACTAAAAGTTTCAAATATAAAGCTTCAGTCGTCTGAGAATGAGGTAGCTAGATTAAAGAGTGAGGTTGAGAAGAATGAAACAGCAATTTTGCTATCAGAAGGCTTGCAGGCTCAGCTTGAGTCGGCTGAAAAAGACAAGCAACAAATGGAGACCGATCTCCAAGTAGAAAAAGGGCGAGTTCTGGAGCTGGAACGTCAAATAGAACAATTGGAAACTCGGAGCTTAGAATCTAATTCCAAAATTGAGAGATTGAGTGAAGAGATAGAATCATGCAAAGAAATTATCAAGAGTTCAGATGACAAGACCACAAGGCTTACGCATGAACTCGAAACTACAAAATCTGACCACCATTTTCAGATCAAGGAATTGGAAACTGCTTTTCAAGTTTCACAGGAGCGATTCCATGCTGAGAAAGAACAGATGCAGACAGATATTCTCAGGCGAATTGAAGCTGATAAAACTGAAATGAGAGCCCTCCACAGCGCTCACGAGACAGCTTTGCAAAGCGAGATTAGTCAGTTAAAGGAAGAACTTTTTTCAAGAAGCGAAAGTTTGGCAGCCTTGAATAGAACCCACGACGAACTCAAGCTCAAATATGATATGCTAATGGCTGAGAAAGATGACATGAGTGCCATGGTTAACACACTTCTAGCAGAAAAAGAATCCAGAAAATCTCAGATCCAAGAATTAGAGGATCACCTTCAAAATCTACAAGCAGAGAAGGAGGGCTTGATTGCAGGATTTGAAAGCCAGAACAAACAAATTGATGATTTGAAATCGAAACTCTCAGCGTTGCAGGAAGAAGTGAAAATTCAAAAAACTATGTTAGAGGATGGGGCTAAGGGGAAAAAGGAGGCCATAAGGCAGCTCTGTTTCTCTTTGGAGCACTACAGAAGTGGCTATCAAGAGCTTCGTGAAGCATTGATTGGGCACAAGCCGCGTCCAGCACTCTCTGCGTTGGTCGGTTGCTTGTAAGATCCCTCGAACTTACTACCATGTCCGATTTTCTGTTCTATATGCTTTTTTTTCTAATCTTGTATCCTTTTTTTTGAGTTTATGAGCATCATTATTTAGTTGATTTTTTAACGTAGGTCAAGAACAATTGAATATTATGTTAAAAAATTTAGACCAGTTGTAAGGATGGACATTTGACGGCTTTGGTTTAACATTGAGCATCCAAAAAGCTGCATATATTTGTTGGAGCATTATCATGCTTCCACCTGCA

mRNA sequence

ATGACTCGGAATGTTGAGCAGATGCTGAAGGCGATGAAAGAAAATAGTGATAATTTTCCGAAAAGTGCTGATATGGATTCTCAAGTTGAGGAGTTCTCTCGCTTGTATCAATCGTTGGTTGAAAACGTGTTATCGCCGGAGCTACAATTGCAGGTACCTGTTTATTCTGATTGTGGATCTCCTCGAGGCACTCCGGAATTAAGTTTCAACCAAAAACAGGATTTCAATATGTCCAGCAATAGAGGCTTGGATGTCTCTTTTGATTCAGGTGGTGGTAGCTCCAGCCTTTCTCTGAAGGACGGAAGTGAATCTTCTTCTTCTTCATCGGACTCTGAATTAGAATCTGTCGACAATCACTATGCGGTTTCACGTGATCAAAGAGATGGTCAAGGGCAGAAGAAAAAACTTCTTGAGCTACAAACTGAGCTTCCTCCTAAAATTAGAGGGGCATTATGGGGGGATGAGGAAGACAAGGTAAATTATGATGAGTTGTACGAAAGAATCACCAGGCTTGAGGAAGAACTAAAAGTTTCAAATATAAAGCTTCAGTCGTCTGAGAATGAGGTAGCTAGATTAAAGAGTGAGGTTGAGAAGAATGAAACAGCAATTTTGCTATCAGAAGGCTTGCAGGCTCAGCTTGAGTCGGCTGAAAAAGACAAGCAACAAATGGAGACCGATCTCCAAGTAGAAAAAGGGCGAGTTCTGGAGCTGGAACGTCAAATAGAACAATTGGAAACTCGGAGCTTAGAATCTAATTCCAAAATTGAGAGATTGAGTGAAGAGATAGAATCATGCAAAGAAATTATCAAGAGTTCAGATGACAAGACCACAAGGCTTACGCATGAACTCGAAACTACAAAATCTGACCACCATTTTCAGATCAAGGAATTGGAAACTGCTTTTCAAGTTTCACAGGAGCGATTCCATGCTGAGAAAGAACAGATGCAGACAGATATTCTCAGGCGAATTGAAGCTGATAAAACTGAAATGAGAGCCCTCCACAGCGCTCACGAGACAGCTTTGCAAAGCGAGATTAGTCAGTTAAAGGAAGAACTTTTTTCAAGAAGCGAAAGTTTGGCAGCCTTGAATAGAACCCACGACGAACTCAAGCTCAAATATGATATGCTAATGGCTGAGAAAGATGACATGAGTGCCATGGTTAACACACTTCTAGCAGAAAAAGAATCCAGAAAATCTCAGATCCAAGAATTAGAGGATCACCTTCAAAATCTACAAGCAGAGAAGGAGGGCTTGATTGCAGGATTTGAAAGCCAGAACAAACAAATTGATGATTTGAAATCGAAACTCTCAGCGTTGCAGGAAGAAGTGAAAATTCAAAAAACTATGTTAGAGGATGGGGCTAAGGGGAAAAAGGAGGCCATAAGGCAGCTCTGTTTCTCTTTGGAGCACTACAGAAGTGGCTATCAAGAGCTTCGTGAAGCATTGATTGGGCACAAGCCGCGTCCAGCACTCTCTGCGTTGGTCGGTTGCTTGTAAGATCCCTCGAACTTACTACCATGTCCGATTTTCTGTTCTATATGCTTTTTTTTCTAATCTTGTATCCTTTTTTTTGAGTTTATGAGCATCATTATTTAGTTGATTTTTTAACGTAGGTCAAGAACAATTGAATATTATGTTAAAAAATTTAGACCAGTTGTAAGGATGGACATTTGACGGCTTTGGTTTAACATTGAGCATCCAAAAAGCTGCATATATTTGTTGGAGCATTATCATGCTTCCACCTGCA

Coding sequence (CDS)

ATGACTCGGAATGTTGAGCAGATGCTGAAGGCGATGAAAGAAAATAGTGATAATTTTCCGAAAAGTGCTGATATGGATTCTCAAGTTGAGGAGTTCTCTCGCTTGTATCAATCGTTGGTTGAAAACGTGTTATCGCCGGAGCTACAATTGCAGGTACCTGTTTATTCTGATTGTGGATCTCCTCGAGGCACTCCGGAATTAAGTTTCAACCAAAAACAGGATTTCAATATGTCCAGCAATAGAGGCTTGGATGTCTCTTTTGATTCAGGTGGTGGTAGCTCCAGCCTTTCTCTGAAGGACGGAAGTGAATCTTCTTCTTCTTCATCGGACTCTGAATTAGAATCTGTCGACAATCACTATGCGGTTTCACGTGATCAAAGAGATGGTCAAGGGCAGAAGAAAAAACTTCTTGAGCTACAAACTGAGCTTCCTCCTAAAATTAGAGGGGCATTATGGGGGGATGAGGAAGACAAGGTAAATTATGATGAGTTGTACGAAAGAATCACCAGGCTTGAGGAAGAACTAAAAGTTTCAAATATAAAGCTTCAGTCGTCTGAGAATGAGGTAGCTAGATTAAAGAGTGAGGTTGAGAAGAATGAAACAGCAATTTTGCTATCAGAAGGCTTGCAGGCTCAGCTTGAGTCGGCTGAAAAAGACAAGCAACAAATGGAGACCGATCTCCAAGTAGAAAAAGGGCGAGTTCTGGAGCTGGAACGTCAAATAGAACAATTGGAAACTCGGAGCTTAGAATCTAATTCCAAAATTGAGAGATTGAGTGAAGAGATAGAATCATGCAAAGAAATTATCAAGAGTTCAGATGACAAGACCACAAGGCTTACGCATGAACTCGAAACTACAAAATCTGACCACCATTTTCAGATCAAGGAATTGGAAACTGCTTTTCAAGTTTCACAGGAGCGATTCCATGCTGAGAAAGAACAGATGCAGACAGATATTCTCAGGCGAATTGAAGCTGATAAAACTGAAATGAGAGCCCTCCACAGCGCTCACGAGACAGCTTTGCAAAGCGAGATTAGTCAGTTAAAGGAAGAACTTTTTTCAAGAAGCGAAAGTTTGGCAGCCTTGAATAGAACCCACGACGAACTCAAGCTCAAATATGATATGCTAATGGCTGAGAAAGATGACATGAGTGCCATGGTTAACACACTTCTAGCAGAAAAAGAATCCAGAAAATCTCAGATCCAAGAATTAGAGGATCACCTTCAAAATCTACAAGCAGAGAAGGAGGGCTTGATTGCAGGATTTGAAAGCCAGAACAAACAAATTGATGATTTGAAATCGAAACTCTCAGCGTTGCAGGAAGAAGTGAAAATTCAAAAAACTATGTTAGAGGATGGGGCTAAGGGGAAAAAGGAGGCCATAAGGCAGCTCTGTTTCTCTTTGGAGCACTACAGAAGTGGCTATCAAGAGCTTCGTGAAGCATTGATTGGGCACAAGCCGCGTCCAGCACTCTCTGCGTTGGTCGGTTGCTTGTAA

Protein sequence

MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGSPRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELESVDNHYAVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQIEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETAFQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLAALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELREALIGHKPRPALSALVGCL
Homology
BLAST of CmaCh04G028490 vs. ExPASy Swiss-Prot
Match: F4KEW8 (Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 6.6e-21
Identity = 151/542 (27.86%), Postives = 265/542 (48.89%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQ--------VEEFSRLYQSLV---ENVL----- 60
           M R+V++M+K ++E++D+F K A+M  Q        V+EF R+Y++L    EN+      
Sbjct: 44  MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 103

Query: 61  -SP-ELQLQVPVYSDCGSPRGTPELSFNQKQDFNM--SSNR--GLDVSFDSGGGSSSLSL 120
            SP ELQ Q    SD  +   +   + N+        S  R  G +    +GG  S L  
Sbjct: 104 GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 163

Query: 121 KDGSESSSSSSDSELE----SVDNHYAVSRDQRDGQGQKKKLLELQTEL---PPKIRGAL 180
           KDG + S+S +DSELE    SV N+        D Q   K++++L+ EL     ++R  L
Sbjct: 164 KDG-DDSASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRMQL 223

Query: 181 WGDEE-------DKVNYDELYERITRLEEELKVSNIKLQSSENEVARLKSEVEKNETAIL 240
            G+ E        +  + +   ++   E+ELK  N KLQ+SE+++  LKS++ +   + L
Sbjct: 224 EGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARYLPSGL 283

Query: 241 LSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQ---IEQLETRSLESNSKIERLSEE 300
             E  +    + E D + +  +L++   R+ E E+Q   + +   +S   ++K++ L + 
Sbjct: 284 DDEQSEGAASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQDM 343

Query: 301 IESCKEII-----KSSDDK--TTRLTHELETTKS-----DHHFQIKELETAFQVSQERFH 360
           +ES ++       K+S DK    +L   +   KS     DH  +I++L+TA   ++E+  
Sbjct: 344 LESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDH--EIRDLKTALSDAEEKIF 403

Query: 361 AEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLAALNRTHDEL 420
            EK Q++ DI + +E        +H              +++ F   E  A +    DE 
Sbjct: 404 PEKAQVKADIAKLLE------EKIH--------------RDDQFKELE--ANVRYLEDER 463

Query: 421 KLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQI 480
           +   +  + E++ + + +  L  EK  +   I+ L   +  L++E    I+   S+ K  
Sbjct: 464 RKVNNEKIEEEEKLKSEIEVLTLEKVEKGRCIETLSRKVSELESE----ISRLGSEIKAR 523

Query: 481 DDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELREALIGHKP-R 491
           DD   +   +++EV+ Q+  LE+ A+ K+E IRQLCFSL++ R  Y+ LR A  GH P R
Sbjct: 524 DD---RTMEMEKEVEKQRRELEEVAEEKREVIRQLCFSLDYSRDEYKRLRIAFSGHPPTR 553

BLAST of CmaCh04G028490 vs. ExPASy Swiss-Prot
Match: Q84VY2 (Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 4.3e-20
Identity = 143/518 (27.61%), Postives = 234/518 (45.17%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQ--------VEEFSRLYQSLVENVLSPELQLQV 60
           M   V  MLK ++E++D+F K A M  Q        VEEF R+Y++L E       +LQ 
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  PVYSDCGSPRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSE 120
              S+  S + + E+S   K+  +                SS    +D S  + S SDS+
Sbjct: 104 NHTSEIQS-QSSLEISSPTKEKLSRRQ-------------SSHKEEEDSSSLTDSGSDSD 163

Query: 121 LESVDNHYAVSRDQRDGQGQKKKLLELQTEL-PPKIRGALWGDEEDKVNYDELYERITRL 180
                  ++ + D+   +   +++ EL+ EL   K +  L  +  D  N  +L  +IT  
Sbjct: 164 -------HSSANDEDGDEALIRRMAELELELQETKQKLLLQQESVDGDNNVDLLHKITTY 223

Query: 181 EEELKVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEK 240
           E ELK +N K++  E+E+A LK++++ +  +    + L A+ +S + DK+  + D    K
Sbjct: 224 EGELKEANEKMRMHEDEIANLKNQLQ-SFMSFDTEDHLGAEQKSVDLDKEDTKEDAVATK 283

Query: 241 GRVLELERQIEQLETRSLESNSKIERLSEEIESCK---EIIKSSDDKTTRLTHELETTKS 300
              LE E  I         +  K++   +E  S K   EI K++++K   L HELE  + 
Sbjct: 284 VLALEEELSI---------AKEKLQHFEKETYSLKNELEIGKAAEEKLKSLQHELELAQR 343

Query: 301 DHHFQIKELETAFQVSQERFHAEKEQMQTDILRRIEADK-TEMRALHSAHETALQS---E 360
           D    I +L      + E+    K Q +  +++    D+  E+RAL +A   A Q    E
Sbjct: 344 DADTYINKL------NAEKKEVLKLQERLAMVKTSLQDRDNEIRALKTAVSDAEQKIFPE 403

Query: 361 ISQLKEELFSRSESLAALNRTHDELKLKYDMLMAEKDD--------------MSAMVNTL 420
            +Q+K E+    E  + L     EL+    ++  EK +              M    N L
Sbjct: 404 KAQIKGEMSKMLEERSQLGEQLRELESHIRLIKEEKAETEEKLRGGTEKISGMRDESNVL 463

Query: 421 LAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQIDDLKSKLSALQEEVKIQKTML 480
             E   R+ +I+E E H++ L  E+                L+ + S L EEV+  +   
Sbjct: 464 REEIGKREEKIKETEKHMEELHMEQV--------------RLRRRSSELTEEVERTRVSA 510

Query: 481 EDGAKGKKEAIRQLCFSLEHYRSGYQELREALIGHKPR 489
            + A+ K+EAIRQLC SL+HYR GY  L   + GHK +
Sbjct: 524 SEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSK 510

BLAST of CmaCh04G028490 vs. ExPASy Swiss-Prot
Match: F4JZY1 (COP1-interactive protein 1 OS=Arabidopsis thaliana OX=3702 GN=CIP1 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 5.4e-07
Identity = 96/387 (24.81%), Postives = 181/387 (46.77%), Query Frame = 0

Query: 101 GSESSSSSSDSELESVDNHYAVSRDQRDGQGQKKKLLELQT-ELPPKIRGALWGDEEDKV 160
           G  SSSSSSDS+     +H +  + +R+G G+ +K +EL T  L  +I  A     + K 
Sbjct: 92  GESSSSSSSDSD----SDHSSKRKVKRNGNGKVEKDVELVTGALKQQIEAANLEIADLKG 151

Query: 161 NYDELYERITRLEEELKVSNIKLQSSENEVARLKSEVEK--NETAILLSEG--------- 220
                 E    ++ EL+++ +KL+ SE   ++LK E EK  +E +I LS+          
Sbjct: 152 KLTTTVEEKEAVDSELELALMKLKESEEISSKLKLETEKLEDEKSIALSDNRELHQKLEV 211

Query: 221 -------LQAQLESAEKDKQQMETDLQVEKGRVLELERQIEQLETRSLESNSKIERLSEE 280
                  L  +LE  +K++ +++T+      R  E E+  E  +T S       ++L +E
Sbjct: 212 AGKTETDLNQKLEDIKKERDELQTERDNGIKRFQEAEKVAEDWKTTS-------DQLKDE 271

Query: 281 IESCKEIIKSSDDKTTRLTHELETTKSDH---HFQIKELETAFQVSQ---ERFHAEKEQM 340
             + K+ +++S+ + + LT  + + + ++     ++ E+    Q  Q   +   +E  +M
Sbjct: 272 TSNLKQQLEASEQRVSELTSGMNSAEEENKSLSLKVSEISDVIQQGQTTIQELISELGEM 331

Query: 341 QTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLA----ALNRTHDELKL 400
           + +  +  E++ + +  LH  HE    S++ +L+  + S  + +A    +LN   +E KL
Sbjct: 332 K-EKYKEKESEHSSLVELHKTHERESSSQVKELEAHIESSEKLVADFTQSLNNAEEEKKL 391

Query: 401 KYDMLMAEKDDMSAMVNT---LLAE----KESRKSQIQEL-------EDHLQNLQAEKEG 445
               +    +++    NT   L++E    KES   + +EL       E H ++       
Sbjct: 392 LSQKIAELSNEIQEAQNTMQELMSESGQLKESHSVKERELFSLRDIHEIHQRDSSTRASE 451

BLAST of CmaCh04G028490 vs. ExPASy Swiss-Prot
Match: P10587 (Myosin-11 OS=Gallus gallus OX=9031 GN=MYH11 PE=1 SV=4)

HSP 1 Score: 55.5 bits (132), Expect = 2.1e-06
Identity = 86/331 (25.98%), Postives = 156/331 (47.13%), Query Frame = 0

Query: 163  ELYERITRLEEELKVSNIKLQS-----SENEVARLKSEVEKNETAILLSEGLQAQLESAE 222
            EL ++ T+L EE  +   KLQ+     +E E  R++   +K E   +L E ++A++E  E
Sbjct: 883  ELEQKHTQLCEEKNLLQEKLQAETELYAEAEEMRVRLAAKKQELEEILHE-MEARIEEEE 942

Query: 223  KDKQQMETDLQVEKGRVLELERQIEQ-------LETRSLESNSKIERLSEEI-------- 282
            +  QQ++ + +  + ++L+LE Q+E+       L+   + ++ KI+++ ++I        
Sbjct: 943  ERSQQLQAEKKKMQQQMLDLEEQLEEEEAARQKLQLEKVTADGKIKKMEDDILIMEDQNN 1002

Query: 283  ---ESCKEIIKSSDDKTTRLTHE------LETTKSDHHFQIKELETAFQVSQERFHAEKE 342
               +  K + +   D TT L  E      L   K+ H   I ELE   +  +E+   E E
Sbjct: 1003 KLTKERKLLEERVSDLTTNLAEEEEKAKNLTKLKNKHESMISELEVRLK-KEEKSRQELE 1062

Query: 343  QMQTDILRRIEADKTEMRALHSAHE--TALQSEISQLKEELFSRSESL-AALNRTHDELK 402
            +    I R++E + +++      HE    LQ++I++LK +L  + E L AAL R  DE  
Sbjct: 1063 K----IKRKLEGESSDL------HEQIAELQAQIAELKAQLAKKEEELQAALARLEDE-- 1122

Query: 403  LKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQID 460
                                 ++K +   +I+ELE H+ +LQ + E   A      KQ  
Sbjct: 1123 --------------------TSQKNNALKKIRELESHISDLQEDLESEKAARNKAEKQKR 1179

BLAST of CmaCh04G028490 vs. ExPASy Swiss-Prot
Match: O08638 (Myosin-11 OS=Mus musculus OX=10090 GN=Myh11 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.9e-05
Identity = 88/362 (24.31%), Postives = 170/362 (46.96%), Query Frame = 0

Query: 122  VSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNIK 181
            V+R + + Q +++++ ++ TE   K    L   +E +  + +L E  T L+E+L+     
Sbjct: 847  VTRQEEEMQAKEEEMQKI-TERQQKAETEL---KELEQKHTQLAEEKTLLQEQLQAETEL 906

Query: 182  LQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQI 241
               +E+E  R++   +K E   +L E ++A+LE  E  +QQ++ + +    ++L+LE Q+
Sbjct: 907  Y--AESEEMRVRLAAKKQELEEILHE-MEARLEEEEDRRQQLQAERKKMAQQMLDLEEQL 966

Query: 242  EQ-------LETRSLESNSKIERLSEEI-----------ESCKEIIKSSDDKTTRLTHEL 301
            E+       L+   + + +KI++L ++I           +  K + +   D TT L  E 
Sbjct: 967  EEEEAARQKLQLEKVTAEAKIKKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEE 1026

Query: 302  ETTKSDHHFQIKELETAFQVSQERFHAEKEQMQTDIL-RRIEADKTEMRALHSAHE--TA 361
            E  K+    + K      ++       EK + + + L R++E D ++       HE    
Sbjct: 1027 EKAKNLTKLKSKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDF------HEQIAD 1086

Query: 362  LQSEISQLKEELFSRSESL-AALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKS 421
            LQ++I++LK +L  + E L AAL R  +E                      +A+K +   
Sbjct: 1087 LQAQIAELKMQLAKKEEELQAALARLDEE----------------------IAQKNNALK 1146

Query: 422  QIQELEDHLQNLQAEKEGLIAGFESQNKQIDDLKSKLSALQEEVK--IQKTMLEDGAKGK 460
            +I+ELE H+ +LQ + +   A      KQ  DL  +L AL+ E++  +  T  +   + K
Sbjct: 1147 KIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAK 1173

BLAST of CmaCh04G028490 vs. ExPASy TrEMBL
Match: A0A6J1JQP9 (protein NETWORKED 4A-like OS=Cucurbita maxima OX=3661 GN=LOC111486963 PE=4 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.3e-261
Identity = 498/498 (100.00%), Postives = 498/498 (100.00%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS
Sbjct: 32  MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 91

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELESVDNHY 120
           PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELESVDNHY
Sbjct: 92  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELESVDNHY 151

Query: 121 AVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNI 180
           AVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNI
Sbjct: 152 AVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNI 211

Query: 181 KLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQ 240
           KLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQ
Sbjct: 212 KLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQ 271

Query: 241 IEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETA 300
           IEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETA
Sbjct: 272 IEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETA 331

Query: 301 FQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLA 360
           FQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLA
Sbjct: 332 FQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLA 391

Query: 361 ALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIA 420
           ALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIA
Sbjct: 392 ALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIA 451

Query: 421 GFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELRE 480
           GFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELRE
Sbjct: 452 GFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELRE 511

Query: 481 ALIGHKPRPALSALVGCL 499
           ALIGHKPRPALSALVGCL
Sbjct: 512 ALIGHKPRPALSALVGCL 529

BLAST of CmaCh04G028490 vs. ExPASy TrEMBL
Match: A0A6J1EC39 (protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111431199 PE=4 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 9.9e-246
Identity = 475/503 (94.43%), Postives = 485/503 (96.42%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLV NVLSPELQLQVPVYSDCGS
Sbjct: 32  MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVGNVLSPELQLQVPVYSDCGS 91

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE-----SSSSSSDSELES 120
           P+GTPELS NQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE     SSSSSSDSELES
Sbjct: 92  PQGTPELSLNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSSSSSSDSELES 151

Query: 121 VDNHYAVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEEL 180
           VDN YAVSRDQRDGQGQKKKLLELQTELP KIRGALWGDEEDKVNYDELYERI RLEEEL
Sbjct: 152 VDNPYAVSRDQRDGQGQKKKLLELQTELPSKIRGALWGDEEDKVNYDELYERIARLEEEL 211

Query: 181 KVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVL 240
           KVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDL+VEK RVL
Sbjct: 212 KVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLRVEKRRVL 271

Query: 241 ELERQIEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIK 300
           ELERQIEQLETRSLESNSKIERLSEEIE+CKEIIKSSDDKTTRLTHELETTKS+HHFQIK
Sbjct: 272 ELERQIEQLETRSLESNSKIERLSEEIETCKEIIKSSDDKTTRLTHELETTKSNHHFQIK 331

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSR 360
           ELETAFQVSQERFHAEKEQMQ DILR++EADKTEMRALH+AHETALQ EISQLKEELFSR
Sbjct: 332 ELETAFQVSQERFHAEKEQMQIDILRQVEADKTEMRALHNAHETALQREISQLKEELFSR 391

Query: 361 SESLAALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEK 420
           SESLAALNRTHDELKLKYDMLMAEKDD+SAMVNTLLA+KESRKSQ+QELEDHLQ LQAEK
Sbjct: 392 SESLAALNRTHDELKLKYDMLMAEKDDISAMVNTLLADKESRKSQVQELEDHLQILQAEK 451

Query: 421 EGLIAGFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGY 480
           EGLIA  ESQNKQIDDLKSKLS LQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGY
Sbjct: 452 EGLIARSESQNKQIDDLKSKLSELQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGY 511

Query: 481 QELREALIGHKPRPALSALVGCL 499
           QELREA IGHKPRPALSALVGCL
Sbjct: 512 QELREAFIGHKPRPALSALVGCL 534

BLAST of CmaCh04G028490 vs. ExPASy TrEMBL
Match: A0A6J1JXC2 (protein NETWORKED 4A OS=Cucurbita maxima OX=3661 GN=LOC111489163 PE=4 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 6.5e-189
Identity = 374/497 (75.25%), Postives = 432/497 (86.92%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           M R+VEQMLK +K+NS+NFPKSADMDSQVEEFSRLYQSL+EN+LSPELQ QVP YSDCGS
Sbjct: 32  MNRSVEQMLKTIKDNSNNFPKSADMDSQVEEFSRLYQSLLENLLSPELQPQVPFYSDCGS 91

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELE----SV 120
           P+ TPELS +QKQ FN+S NRGLD+S DSGGGSSSLSLKDG+ SSSSSSDSE E    SV
Sbjct: 92  PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGTGSSSSSSDSESESFSSSV 151

Query: 121 DNHYAVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELK 180
           DN+Y VSR +RDGQG+KKK L L+TEL   I+GA W  EEDK+NYDEL+E+I R+EEELK
Sbjct: 152 DNNYVVSRAERDGQGRKKKFLGLETEL-SNIKGAFWVGEEDKLNYDELHEKIARIEEELK 211

Query: 181 VSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLE 240
           VSN KLQSSENEVARLKSE+EKNETA+ LSEGLQAQLESAEKDKQ+M  DLQVEK RV+E
Sbjct: 212 VSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVEKRRVVE 271

Query: 241 LERQIEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKE 300
           LE+QIEQLETR L+S+SKIE L EE+E  +EI+KSSDDK  RLTHELE TKSDH  QIKE
Sbjct: 272 LEQQIEQLETRVLQSDSKIEILIEELEMSREILKSSDDKIARLTHELEITKSDHRLQIKE 331

Query: 301 LETAFQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRS 360
           LE+AFQVSQE F+AEKEQMQ D+LR++EADKTE+RALH++H T+LQ EISQLKEELF +S
Sbjct: 332 LESAFQVSQEIFNAEKEQMQADVLRQVEADKTEIRALHNSHLTSLQGEISQLKEELFVKS 391

Query: 361 ESLAALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKE 420
           +SL+ALN  HDELKLKY+MLMAEKD+ +AMV+TL+A+KESR+S + ELE HLQ+LQ E+E
Sbjct: 392 QSLSALNSNHDELKLKYNMLMAEKDEATAMVHTLVADKESRESHVHELESHLQHLQEEEE 451

Query: 421 GLIAGFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQ 480
           GLIAG E QNKQIDDLK +LS +QEEV  Q  M+EDGA+ K+EAIRQLCFSLEHYRSGY+
Sbjct: 452 GLIAGSEIQNKQIDDLKLRLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGYK 511

Query: 481 ELREALIGHKPRPALSA 494
           ELREA IGHKPR  LSA
Sbjct: 512 ELREAFIGHKPRQVLSA 527

BLAST of CmaCh04G028490 vs. ExPASy TrEMBL
Match: A0A6J1FN40 (protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111445431 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 3.0e-186
Identity = 372/496 (75.00%), Postives = 424/496 (85.48%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           M R+VEQMLK +K+N DNFPKSADMDSQVEEFSRLYQSL+EN+LSPELQ QVP YSDCGS
Sbjct: 32  MNRSVEQMLKTIKDNCDNFPKSADMDSQVEEFSRLYQSLLENLLSPELQPQVPFYSDCGS 91

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELE----SV 120
           P+ TPELS +QKQ FN+S NRGLD+S DSGGGSSSLSLKDG+ SSSSSSDSE E    SV
Sbjct: 92  PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGTGSSSSSSDSESESFSSSV 151

Query: 121 DNHYAVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELK 180
           DN+Y VSR +RDGQG KKK L L+TEL   I GA W  EEDK+NY EL+E+I R+EEELK
Sbjct: 152 DNNYVVSRAERDGQGPKKKFLGLETEL-SNIEGAFWVGEEDKLNYHELHEKIARIEEELK 211

Query: 181 VSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLE 240
           VSN KLQSSENEVARLKSE+EKNETA+ LSEGLQAQLESAEKDKQ+M  DLQV K RV+E
Sbjct: 212 VSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVLKRRVVE 271

Query: 241 LERQIEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKE 300
           LE+QIEQLETR L+S+SKIE L EE+E  +EI+KSSDDK  RLTHELE TKSDH  QIKE
Sbjct: 272 LEQQIEQLETRVLQSDSKIEILIEELEMSREILKSSDDKIARLTHELEITKSDHRLQIKE 331

Query: 301 LETAFQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRS 360
           LE+AFQVSQE F+AEKEQ+Q D+LR++EADKTEMRALH++H T+LQ EISQLKEEL  +S
Sbjct: 332 LESAFQVSQELFNAEKEQVQADVLRQVEADKTEMRALHNSHLTSLQGEISQLKEELLVKS 391

Query: 361 ESLAALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKE 420
           +SL+ALN  HDELKLKYDMLMAEKD+ +AMV+TL+A+KE R+S + ELE HLQ+LQ EKE
Sbjct: 392 QSLSALNSNHDELKLKYDMLMAEKDEATAMVHTLVADKEYRESHVHELESHLQHLQEEKE 451

Query: 421 GLIAGFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQ 480
           GLIAG ESQNKQIDDLK  LS +QEEV  Q  M+EDGA+ K+EAIRQLCFSLEHYRSGY+
Sbjct: 452 GLIAGSESQNKQIDDLKLGLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGYK 511

Query: 481 ELREALIGHKPRPALS 493
           ELREA IGHKPR  LS
Sbjct: 512 ELREAFIGHKPRQVLS 526

BLAST of CmaCh04G028490 vs. ExPASy TrEMBL
Match: A0A0A0KXR2 (NAB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G645160 PE=4 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 2.6e-185
Identity = 369/499 (73.95%), Postives = 427/499 (85.57%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           M R+VEQMLK +KENSD+FP+S DMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS
Sbjct: 120 MNRSVEQMLKTIKENSDSFPESVDMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 179

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELE------ 120
           P+GTPELS +QKQ FN+SSNRGLD+SFDSGGGSSSLSLKDG+ESSSSSS S+ E      
Sbjct: 180 PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 239

Query: 121 SVDNHYAVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEE 180
           SVDN+Y VSR +RDGQG KKKLL +++EL P I+G  W  EE KVNYDEL+++I ++EEE
Sbjct: 240 SVDNNYVVSRAERDGQGLKKKLLAIESEL-PNIKGGFWVGEEVKVNYDELHDKIAKIEEE 299

Query: 181 LKVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRV 240
           LKVSN KLQSSENEV RLKSEVEKNETAILLSEGLQAQLES EKDKQ MET+LQV+K   
Sbjct: 300 LKVSNAKLQSSENEVTRLKSEVEKNETAILLSEGLQAQLESVEKDKQVMETELQVKK--- 359

Query: 241 LELERQIEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQI 300
               ++IEQLETR  +S+SKIERL +++E  KE++KSS+DK TRLTHELE TKSDHH QI
Sbjct: 360 ----KKIEQLETRISQSDSKIERLIKDLEISKEMLKSSNDKITRLTHELENTKSDHHIQI 419

Query: 301 KELETAFQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFS 360
           KELETAFQVSQERFHAEKEQM+TDILR++EA+KTE +ALH++  T  Q EISQLKEEL  
Sbjct: 420 KELETAFQVSQERFHAEKEQMETDILRQVEAEKTETKALHNSQLTMYQGEISQLKEELSV 479

Query: 361 RSESLAALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAE 420
           RSESLA LNR HDELKLKYDM+MAEKD+ SA+V +LLA+KESR+S + EL+  L++LQ +
Sbjct: 480 RSESLADLNRNHDELKLKYDMVMAEKDEASAVVLSLLADKESRESHVNELKGQLKHLQVD 539

Query: 421 KEGLIAGFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSG 480
           K  LI G E QNK+IDDLK +LS L EEVK+QKTM+EDGA+GK+EAIRQLCFSLEHYR+G
Sbjct: 540 KRRLIEGSERQNKEIDDLKLRLSELGEEVKMQKTMIEDGAEGKREAIRQLCFSLEHYRNG 599

Query: 481 YQELREALIGHKPRPALSA 494
           YQELREA I HKPRP LSA
Sbjct: 600 YQELREAFIAHKPRPVLSA 610

BLAST of CmaCh04G028490 vs. NCBI nr
Match: XP_022989923.1 (protein NETWORKED 4A-like [Cucurbita maxima] >XP_022989924.1 protein NETWORKED 4A-like [Cucurbita maxima])

HSP 1 Score: 911.8 bits (2355), Expect = 2.7e-261
Identity = 498/498 (100.00%), Postives = 498/498 (100.00%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS
Sbjct: 32  MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 91

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELESVDNHY 120
           PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELESVDNHY
Sbjct: 92  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELESVDNHY 151

Query: 121 AVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNI 180
           AVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNI
Sbjct: 152 AVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNI 211

Query: 181 KLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQ 240
           KLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQ
Sbjct: 212 KLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQ 271

Query: 241 IEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETA 300
           IEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETA
Sbjct: 272 IEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETA 331

Query: 301 FQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLA 360
           FQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLA
Sbjct: 332 FQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLA 391

Query: 361 ALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIA 420
           ALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIA
Sbjct: 392 ALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIA 451

Query: 421 GFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELRE 480
           GFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELRE
Sbjct: 452 GFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELRE 511

Query: 481 ALIGHKPRPALSALVGCL 499
           ALIGHKPRPALSALVGCL
Sbjct: 512 ALIGHKPRPALSALVGCL 529

BLAST of CmaCh04G028490 vs. NCBI nr
Match: KAG7033248.1 (Protein NETWORKED 4A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 864.0 bits (2231), Expect = 6.3e-247
Identity = 477/503 (94.83%), Postives = 487/503 (96.82%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLV NVLSPELQLQVPVYSDCGS
Sbjct: 32  MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVGNVLSPELQLQVPVYSDCGS 91

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE-----SSSSSSDSELES 120
           P+GTPELS NQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE     SSSSSSDSELES
Sbjct: 92  PQGTPELSLNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSSSSSSDSELES 151

Query: 121 VDNHYAVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEEL 180
           VDN YAVSRDQRDGQGQKKKLLELQTELP KIRGALWGDEEDKVNYDELYERITRLEEEL
Sbjct: 152 VDNPYAVSRDQRDGQGQKKKLLELQTELPSKIRGALWGDEEDKVNYDELYERITRLEEEL 211

Query: 181 KVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVL 240
           KVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEK RVL
Sbjct: 212 KVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKRRVL 271

Query: 241 ELERQIEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIK 300
           ELERQIEQLETRSLESNSKIERLSEEIE+CKEIIKSSDDKTTRLTHELETTKSDHHFQIK
Sbjct: 272 ELERQIEQLETRSLESNSKIERLSEEIETCKEIIKSSDDKTTRLTHELETTKSDHHFQIK 331

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSR 360
           ELETAFQVSQERFH+EKEQMQ DILR++EADKTEMRALH+AHETALQSEISQLKEELFSR
Sbjct: 332 ELETAFQVSQERFHSEKEQMQIDILRQVEADKTEMRALHNAHETALQSEISQLKEELFSR 391

Query: 361 SESLAALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEK 420
           +ESLAALNRTHDELKLKYDMLMAEKDD+SAMVNTLLA+KESRKSQ+QELEDHLQ LQAEK
Sbjct: 392 NESLAALNRTHDELKLKYDMLMAEKDDISAMVNTLLADKESRKSQVQELEDHLQILQAEK 451

Query: 421 EGLIAGFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGY 480
           EGLIA  ESQNKQIDDLKSKLS LQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGY
Sbjct: 452 EGLIARSESQNKQIDDLKSKLSELQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGY 511

Query: 481 QELREALIGHKPRPALSALVGCL 499
           QELREA IGHKPRPALSALVGCL
Sbjct: 512 QELREAFIGHKPRPALSALVGCL 534

BLAST of CmaCh04G028490 vs. NCBI nr
Match: XP_023553367.1 (protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo] >XP_023553438.1 protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo] >XP_023553521.1 protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.1 bits (2226), Expect = 2.4e-246
Identity = 476/500 (95.20%), Postives = 485/500 (97.00%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS
Sbjct: 32  MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 91

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE--SSSSSSDSELESVDN 120
           PRGTPELS NQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE  SSSSSSDSELESVDN
Sbjct: 92  PRGTPELSLNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSSSDSELESVDN 151

Query: 121 HYAVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEELKVS 180
           HYAVSRDQRDGQGQKKKLLELQTELP KIRGALWGDEEDKVNYDELYERITRLEEELKVS
Sbjct: 152 HYAVSRDQRDGQGQKKKLLELQTELPSKIRGALWGDEEDKVNYDELYERITRLEEELKVS 211

Query: 181 NIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELE 240
           NIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEK RVLELE
Sbjct: 212 NIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKRRVLELE 271

Query: 241 RQIEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELE 300
           RQIEQLET SLESNSKIERLS EIE+CKEIIKSSDDKTTRLTHELETTKSDHHF+IKELE
Sbjct: 272 RQIEQLETWSLESNSKIERLSGEIETCKEIIKSSDDKTTRLTHELETTKSDHHFRIKELE 331

Query: 301 TAFQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSES 360
           TAFQVSQERFHAEKEQMQ DILR++EADKTEMRALH+AHETALQ+EISQLKEELFSRSES
Sbjct: 332 TAFQVSQERFHAEKEQMQIDILRQVEADKTEMRALHNAHETALQNEISQLKEELFSRSES 391

Query: 361 LAALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGL 420
           LAALNRTHDELKLKYDMLMAEKDD+SAMVNTLLA+KESRKSQ+ ELEDHLQ LQAEKEGL
Sbjct: 392 LAALNRTHDELKLKYDMLMAEKDDISAMVNTLLADKESRKSQVHELEDHLQILQAEKEGL 451

Query: 421 IAGFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQEL 480
           IA  ESQNKQIDDLKSKLS LQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQEL
Sbjct: 452 IARSESQNKQIDDLKSKLSELQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQEL 511

Query: 481 REALIGHKPRPALSALVGCL 499
           REA I HKPRPALSALVGCL
Sbjct: 512 REAFIEHKPRPALSALVGCL 531

BLAST of CmaCh04G028490 vs. NCBI nr
Match: XP_022923535.1 (protein NETWORKED 4A-like [Cucurbita moschata] >XP_022923544.1 protein NETWORKED 4A-like [Cucurbita moschata] >XP_022923552.1 protein NETWORKED 4A-like [Cucurbita moschata])

HSP 1 Score: 859.0 bits (2218), Expect = 2.0e-245
Identity = 475/503 (94.43%), Postives = 485/503 (96.42%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLV NVLSPELQLQVPVYSDCGS
Sbjct: 32  MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVGNVLSPELQLQVPVYSDCGS 91

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE-----SSSSSSDSELES 120
           P+GTPELS NQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE     SSSSSSDSELES
Sbjct: 92  PQGTPELSLNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSSSSSSDSELES 151

Query: 121 VDNHYAVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEEL 180
           VDN YAVSRDQRDGQGQKKKLLELQTELP KIRGALWGDEEDKVNYDELYERI RLEEEL
Sbjct: 152 VDNPYAVSRDQRDGQGQKKKLLELQTELPSKIRGALWGDEEDKVNYDELYERIARLEEEL 211

Query: 181 KVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVL 240
           KVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDL+VEK RVL
Sbjct: 212 KVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLRVEKRRVL 271

Query: 241 ELERQIEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIK 300
           ELERQIEQLETRSLESNSKIERLSEEIE+CKEIIKSSDDKTTRLTHELETTKS+HHFQIK
Sbjct: 272 ELERQIEQLETRSLESNSKIERLSEEIETCKEIIKSSDDKTTRLTHELETTKSNHHFQIK 331

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSR 360
           ELETAFQVSQERFHAEKEQMQ DILR++EADKTEMRALH+AHETALQ EISQLKEELFSR
Sbjct: 332 ELETAFQVSQERFHAEKEQMQIDILRQVEADKTEMRALHNAHETALQREISQLKEELFSR 391

Query: 361 SESLAALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEK 420
           SESLAALNRTHDELKLKYDMLMAEKDD+SAMVNTLLA+KESRKSQ+QELEDHLQ LQAEK
Sbjct: 392 SESLAALNRTHDELKLKYDMLMAEKDDISAMVNTLLADKESRKSQVQELEDHLQILQAEK 451

Query: 421 EGLIAGFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGY 480
           EGLIA  ESQNKQIDDLKSKLS LQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGY
Sbjct: 452 EGLIARSESQNKQIDDLKSKLSELQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGY 511

Query: 481 QELREALIGHKPRPALSALVGCL 499
           QELREA IGHKPRPALSALVGCL
Sbjct: 512 QELREAFIGHKPRPALSALVGCL 534

BLAST of CmaCh04G028490 vs. NCBI nr
Match: KAG6602571.1 (hypothetical protein SDJN03_07804, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 806.6 bits (2082), Expect = 1.2e-229
Identity = 456/504 (90.48%), Postives = 466/504 (92.46%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLV NVLSPELQL          
Sbjct: 226 MTRNVEQMLKAMKENSDNFPKSADMDSQVEEFSRLYQSLVGNVLSPELQL---------- 285

Query: 61  PRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE------SSSSSSDSELE 120
                       QDFNMSSNRGLDVSFDSGGGSSSLSLKDGSE      SSSSSSDSELE
Sbjct: 286 ------------QDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSSSSSSSDSELE 345

Query: 121 SVDNHYAVSRDQRDGQGQKKKLLELQTELPPKIRGALWGDEEDKVNYDELYERITRLEEE 180
           SVDN YAVSRDQRDGQGQKKKLLELQTELP KIRGALWGDEEDKVNYDE+YERITRLEEE
Sbjct: 346 SVDNPYAVSRDQRDGQGQKKKLLELQTELPSKIRGALWGDEEDKVNYDEVYERITRLEEE 405

Query: 181 LKVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRV 240
           LKVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEK RV
Sbjct: 406 LKVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKRRV 465

Query: 241 LELERQIEQLETRSLESNSKIERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQI 300
           LELERQIEQLETRSLESNSKIERLSEEIE+CKEIIKSSDDKTTRLTHELETTKSDHHFQI
Sbjct: 466 LELERQIEQLETRSLESNSKIERLSEEIETCKEIIKSSDDKTTRLTHELETTKSDHHFQI 525

Query: 301 KELETAFQVSQERFHAEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFS 360
           KELETAFQVSQERFH+EKEQMQ DILR++EADKTEMRALH+AHETALQSEISQLKEELFS
Sbjct: 526 KELETAFQVSQERFHSEKEQMQIDILRQVEADKTEMRALHNAHETALQSEISQLKEELFS 585

Query: 361 RSESLAALNRTHDELKLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAE 420
           R+ESLAALNRTHDELKLKYDMLMAEKDD+SAMVNTLLA+KESRKSQ+QELEDHLQ LQAE
Sbjct: 586 RNESLAALNRTHDELKLKYDMLMAEKDDISAMVNTLLADKESRKSQVQELEDHLQILQAE 645

Query: 421 KEGLIAGFESQNKQIDDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSG 480
           KEGLIA  ESQNKQIDDLKSKLS LQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSG
Sbjct: 646 KEGLIARSESQNKQIDDLKSKLSELQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSG 705

Query: 481 YQELREALIGHKPRPALSALVGCL 499
           YQELREA IGHKPRPALSALVGCL
Sbjct: 706 YQELREAFIGHKPRPALSALVGCL 707

BLAST of CmaCh04G028490 vs. TAIR 10
Match: AT5G05180.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G10880.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 145.6 bits (366), Expect = 1.1e-34
Identity = 133/444 (29.95%), Postives = 225/444 (50.68%), Query Frame = 0

Query: 78  SSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELESVDNHYAVSRDQRDGQGQKKKLL 137
           +SN  +D+S D  G S  ++ K  S++ +S+ D     V  ++  +    D     +   
Sbjct: 15  NSNSFVDISTDHNGSSPPVTPK--SDTVASNFDQGSSDVSGNHDSNSSYSDLDSDTEAFY 74

Query: 138 ELQTE--LPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNIKLQSSENEVARLKSE 197
                  + P    +     E +++Y+EL ++  + EEEL+ +++KLQ  E E+ +LK E
Sbjct: 75  SSFNHHLVSPGSMDSHDLSPEKQMSYEELMKKYVQCEEELRTTSLKLQEFEQEIEKLK-E 134

Query: 198 VEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQIEQLETRSLESNSKI 257
            EK E+ +L  E L+ + E A+ +    +  ++ E+ RVLE++RQ+  LET   + + K 
Sbjct: 135 TEKKESVVLFGEYLRGEREIAQGEIAIRDIAIETERKRVLEVQRQVVDLETELSDLSFKF 194

Query: 258 ERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETAFQVSQERFHAEKEQM 317
           E L  E E  ++ +  S                    +I +L       Q+ F  EK ++
Sbjct: 195 EHLVNEHEVSRDCLDVS------------------FSEISKLREMLCDCQQNFSIEKTKL 254

Query: 318 QTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLAALNRTHDELKLKYDM 377
             D ++  EA+K EM+      E  LQ+EIS LK +L +R E + ALN+  D+ KL+YDM
Sbjct: 255 -VDQIKHSEAEKMEMQ----RKEVELQAEISALKTDLATRGEHIEALNKDFDKHKLRYDM 314

Query: 378 LMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQIDDLKSK 437
           LMAEKD + A V+ L AE  SR  QIQ++E+ L  L  ++  L++   +    +++LK+ 
Sbjct: 315 LMAEKDGVCAEVDNLKAEMRSRDIQIQQMEEQLNQLVYKQTELVSESGNAKNTVEELKAV 374

Query: 438 LSALQEEV------------------------KIQKTMLEDGAKGKKEAIRQLCFSLEHY 496
           +  L+ EV                        ++Q+  +  G + K+EAIRQLCFSL+HY
Sbjct: 375 VKELEIEVELQSKAKKTVEELRATVWEMEKHAELQRNAISQGEEEKREAIRQLCFSLDHY 432

BLAST of CmaCh04G028490 vs. TAIR 10
Match: AT5G05180.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G10880.1); Has 50151 Blast hits to 28081 proteins in 1838 species: Archae - 860; Bacteria - 6013; Metazoa - 25867; Fungi - 3836; Plants - 2236; Viruses - 90; Other Eukaryotes - 11249 (source: NCBI BLink). )

HSP 1 Score: 136.0 bits (341), Expect = 8.5e-32
Identity = 130/444 (29.28%), Postives = 217/444 (48.87%), Query Frame = 0

Query: 78  SSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSELESVDNHYAVSRDQRDGQGQKKKLL 137
           +SN  +D+S D  G S  ++ K  S++ +S+ D     V  ++  +    D     +   
Sbjct: 15  NSNSFVDISTDHNGSSPPVTPK--SDTVASNFDQGSSDVSGNHDSNSSYSDLDSDTEAFY 74

Query: 138 ELQTE--LPPKIRGALWGDEEDKVNYDELYERITRLEEELKVSNIKLQSSENEVARLKSE 197
                  + P    +     E +++Y+EL ++  + EEEL+ +++KLQ  E E+ +LK E
Sbjct: 75  SSFNHHLVSPGSMDSHDLSPEKQMSYEELMKKYVQCEEELRTTSLKLQEFEQEIEKLK-E 134

Query: 198 VEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQIEQLETRSLESNSKI 257
            EK E+ +L  E L+ + E A+ +    +  ++ E+ RVLE++RQ+  LET   + + K 
Sbjct: 135 TEKKESVVLFGEYLRGEREIAQGEIAIRDIAIETERKRVLEVQRQVVDLETELSDLSFKF 194

Query: 258 ERLSEEIESCKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETAFQVSQERFHAEKEQM 317
           E L  E                   HE                       + F  EK ++
Sbjct: 195 EHLVNE-------------------HE-----------------------QNFSIEKTKL 254

Query: 318 QTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLAALNRTHDELKLKYDM 377
             D ++  EA+K EM+      E  LQ+EIS LK +L +R E + ALN+  D+ KL+YDM
Sbjct: 255 -VDQIKHSEAEKMEMQ----RKEVELQAEISALKTDLATRGEHIEALNKDFDKHKLRYDM 314

Query: 378 LMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQIDDLKSK 437
           LMAEKD + A V+ L AE  SR  QIQ++E+ L  L  ++  L++   +    +++LK+ 
Sbjct: 315 LMAEKDGVCAEVDNLKAEMRSRDIQIQQMEEQLNQLVYKQTELVSESGNAKNTVEELKAV 374

Query: 438 LSALQEEV------------------------KIQKTMLEDGAKGKKEAIRQLCFSLEHY 496
           +  L+ EV                        ++Q+  +  G + K+EAIRQLCFSL+HY
Sbjct: 375 VKELEIEVELQSKAKKTVEELRATVWEMEKHAELQRNAISQGEEEKREAIRQLCFSLDHY 408

BLAST of CmaCh04G028490 vs. TAIR 10
Match: AT5G58320.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 103.6 bits (257), Expect = 4.7e-22
Identity = 151/542 (27.86%), Postives = 265/542 (48.89%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQ--------VEEFSRLYQSLV---ENVL----- 60
           M R+V++M+K ++E++D+F K A+M  Q        V+EF R+Y++L    EN+      
Sbjct: 44  MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 103

Query: 61  -SP-ELQLQVPVYSDCGSPRGTPELSFNQKQDFNM--SSNR--GLDVSFDSGGGSSSLSL 120
            SP ELQ Q    SD  +   +   + N+        S  R  G +    +GG  S L  
Sbjct: 104 GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 163

Query: 121 KDGSESSSSSSDSELE----SVDNHYAVSRDQRDGQGQKKKLLELQTEL---PPKIRGAL 180
           KDG + S+S +DSELE    SV N+        D Q   K++++L+ EL     ++R  L
Sbjct: 164 KDG-DDSASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRMQL 223

Query: 181 WGDEE-------DKVNYDELYERITRLEEELKVSNIKLQSSENEVARLKSEVEKNETAIL 240
            G+ E        +  + +   ++   E+ELK  N KLQ+SE+++  LKS++ +   + L
Sbjct: 224 EGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARYLPSGL 283

Query: 241 LSEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQ---IEQLETRSLESNSKIERLSEE 300
             E  +    + E D + +  +L++   R+ E E+Q   + +   +S   ++K++ L + 
Sbjct: 284 DDEQSEGAASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQDM 343

Query: 301 IESCKEII-----KSSDDK--TTRLTHELETTKS-----DHHFQIKELETAFQVSQERFH 360
           +ES ++       K+S DK    +L   +   KS     DH  +I++L+TA   ++E+  
Sbjct: 344 LESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDH--EIRDLKTALSDAEEKIF 403

Query: 361 AEKEQMQTDILRRIEADKTEMRALHSAHETALQSEISQLKEELFSRSESLAALNRTHDEL 420
            EK Q++ DI + +E        +H              +++ F   E  A +    DE 
Sbjct: 404 PEKAQVKADIAKLLE------EKIH--------------RDDQFKELE--ANVRYLEDER 463

Query: 421 KLKYDMLMAEKDDMSAMVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQI 480
           +   +  + E++ + + +  L  EK  +   I+ L   +  L++E    I+   S+ K  
Sbjct: 464 RKVNNEKIEEEEKLKSEIEVLTLEKVEKGRCIETLSRKVSELESE----ISRLGSEIKAR 523

Query: 481 DDLKSKLSALQEEVKIQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELREALIGHKP-R 491
           DD   +   +++EV+ Q+  LE+ A+ K+E IRQLCFSL++ R  Y+ LR A  GH P R
Sbjct: 524 DD---RTMEMEKEVEKQRRELEEVAEEKREVIRQLCFSLDYSRDEYKRLRIAFSGHPPTR 553

BLAST of CmaCh04G028490 vs. TAIR 10
Match: AT2G30500.1 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 100.9 bits (250), Expect = 3.0e-21
Identity = 143/518 (27.61%), Postives = 234/518 (45.17%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQ--------VEEFSRLYQSLVENVLSPELQLQV 60
           M   V  MLK ++E++D+F K A M  Q        VEEF R+Y++L E       +LQ 
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  PVYSDCGSPRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSE 120
              S+  S + + E+S   K+  +                SS    +D S  + S SDS+
Sbjct: 104 NHTSEIQS-QSSLEISSPTKEKLSRRQ-------------SSHKEEEDSSSLTDSGSDSD 163

Query: 121 LESVDNHYAVSRDQRDGQGQKKKLLELQTEL-PPKIRGALWGDEEDKVNYDELYERITRL 180
                  ++ + D+   +   +++ EL+ EL   K +  L  +  D  N  +L  +IT  
Sbjct: 164 -------HSSANDEDGDEALIRRMAELELELQETKQKLLLQQESVDGDNNVDLLHKITTY 223

Query: 181 EEELKVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEK 240
           E ELK +N K++  E+E+A LK++++ +  +    + L A+ +S + DK+  + D    K
Sbjct: 224 EGELKEANEKMRMHEDEIANLKNQLQ-SFMSFDTEDHLGAEQKSVDLDKEDTKEDAVATK 283

Query: 241 GRVLELERQIEQLETRSLESNSKIERLSEEIESCK---EIIKSSDDKTTRLTHELETTKS 300
              LE E  I         +  K++   +E  S K   EI K++++K   L HELE  + 
Sbjct: 284 VLALEEELSI---------AKEKLQHFEKETYSLKNELEIGKAAEEKLKSLQHELELAQR 343

Query: 301 DHHFQIKELETAFQVSQERFHAEKEQMQTDILRRIEADK-TEMRALHSAHETALQS---E 360
           D    I +L      + E+    K Q +  +++    D+  E+RAL +A   A Q    E
Sbjct: 344 DADTYINKL------NAEKKEVLKLQERLAMVKTSLQDRDNEIRALKTAVSDAEQKIFPE 403

Query: 361 ISQLKEELFSRSESLAALNRTHDELKLKYDMLMAEKDD--------------MSAMVNTL 420
            +Q+K E+    E  + L     EL+    ++  EK +              M    N L
Sbjct: 404 KAQIKGEMSKMLEERSQLGEQLRELESHIRLIKEEKAETEEKLRGGTEKISGMRDESNVL 463

Query: 421 LAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQIDDLKSKLSALQEEVKIQKTML 480
             E   R+ +I+E E H++ L  E+                L+ + S L EEV+  +   
Sbjct: 464 REEIGKREEKIKETEKHMEELHMEQV--------------RLRRRSSELTEEVERTRVSA 510

Query: 481 EDGAKGKKEAIRQLCFSLEHYRSGYQELREALIGHKPR 489
            + A+ K+EAIRQLC SL+HYR GY  L   + GHK +
Sbjct: 524 SEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSK 510

BLAST of CmaCh04G028490 vs. TAIR 10
Match: AT2G30500.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 100.9 bits (250), Expect = 3.0e-21
Identity = 143/518 (27.61%), Postives = 234/518 (45.17%), Query Frame = 0

Query: 1   MTRNVEQMLKAMKENSDNFPKSADMDSQ--------VEEFSRLYQSLVENVLSPELQLQV 60
           M   V  MLK ++E++D+F K A M  Q        VEEF R+Y++L E       +LQ 
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  PVYSDCGSPRGTPELSFNQKQDFNMSSNRGLDVSFDSGGGSSSLSLKDGSESSSSSSDSE 120
              S+  S + + E+S   K+  +                SS    +D S  + S SDS+
Sbjct: 104 NHTSEIQS-QSSLEISSPTKEKLSRRQ-------------SSHKEEEDSSSLTDSGSDSD 163

Query: 121 LESVDNHYAVSRDQRDGQGQKKKLLELQTEL-PPKIRGALWGDEEDKVNYDELYERITRL 180
                  ++ + D+   +   +++ EL+ EL   K +  L  +  D  N  +L  +IT  
Sbjct: 164 -------HSSANDEDGDEALIRRMAELELELQETKQKLLLQQESVDGDNNVDLLHKITTY 223

Query: 181 EEELKVSNIKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQQMETDLQVEK 240
           E ELK +N K++  E+E+A LK++++ +  +    + L A+ +S + DK+  + D    K
Sbjct: 224 EGELKEANEKMRMHEDEIANLKNQLQ-SFMSFDTEDHLGAEQKSVDLDKEDTKEDAVATK 283

Query: 241 GRVLELERQIEQLETRSLESNSKIERLSEEIESCK---EIIKSSDDKTTRLTHELETTKS 300
              LE E  I         +  K++   +E  S K   EI K++++K   L HELE  + 
Sbjct: 284 VLALEEELSI---------AKEKLQHFEKETYSLKNELEIGKAAEEKLKSLQHELELAQR 343

Query: 301 DHHFQIKELETAFQVSQERFHAEKEQMQTDILRRIEADK-TEMRALHSAHETALQS---E 360
           D    I +L      + E+    K Q +  +++    D+  E+RAL +A   A Q    E
Sbjct: 344 DADTYINKL------NAEKKEVLKLQERLAMVKTSLQDRDNEIRALKTAVSDAEQKIFPE 403

Query: 361 ISQLKEELFSRSESLAALNRTHDELKLKYDMLMAEKDD--------------MSAMVNTL 420
            +Q+K E+    E  + L     EL+    ++  EK +              M    N L
Sbjct: 404 KAQIKGEMSKMLEERSQLGEQLRELESHIRLIKEEKAETEEKLRGGTEKISGMRDESNVL 463

Query: 421 LAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQIDDLKSKLSALQEEVKIQKTML 480
             E   R+ +I+E E H++ L  E+                L+ + S L EEV+  +   
Sbjct: 464 REEIGKREEKIKETEKHMEELHMEQV--------------RLRRRSSELTEEVERTRVSA 510

Query: 481 EDGAKGKKEAIRQLCFSLEHYRSGYQELREALIGHKPR 489
            + A+ K+EAIRQLC SL+HYR GY  L   + GHK +
Sbjct: 524 SEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSK 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4KEW86.6e-2127.86Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1[more]
Q84VY24.3e-2027.61Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1[more]
F4JZY15.4e-0724.81COP1-interactive protein 1 OS=Arabidopsis thaliana OX=3702 GN=CIP1 PE=1 SV=1[more]
P105872.1e-0625.98Myosin-11 OS=Gallus gallus OX=9031 GN=MYH11 PE=1 SV=4[more]
O086383.9e-0524.31Myosin-11 OS=Mus musculus OX=10090 GN=Myh11 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JQP91.3e-261100.00protein NETWORKED 4A-like OS=Cucurbita maxima OX=3661 GN=LOC111486963 PE=4 SV=1[more]
A0A6J1EC399.9e-24694.43protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111431199 PE=4 SV=... [more]
A0A6J1JXC26.5e-18975.25protein NETWORKED 4A OS=Cucurbita maxima OX=3661 GN=LOC111489163 PE=4 SV=1[more]
A0A6J1FN403.0e-18675.00protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111445431 PE=4 SV=... [more]
A0A0A0KXR22.6e-18573.95NAB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G645160 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_022989923.12.7e-261100.00protein NETWORKED 4A-like [Cucurbita maxima] >XP_022989924.1 protein NETWORKED 4... [more]
KAG7033248.16.3e-24794.83Protein NETWORKED 4A [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023553367.12.4e-24695.20protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo] >XP_023553438.1 protein N... [more]
XP_022923535.12.0e-24594.43protein NETWORKED 4A-like [Cucurbita moschata] >XP_022923544.1 protein NETWORKED... [more]
KAG6602571.11.2e-22990.48hypothetical protein SDJN03_07804, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT5G05180.11.1e-3429.95unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G05180.28.5e-3229.28unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G58320.24.7e-2227.86Kinase interacting (KIP1-like) family protein [more]
AT2G30500.13.0e-2127.61Kinase interacting (KIP1-like) family protein [more]
AT2G30500.23.0e-2127.61Kinase interacting (KIP1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 206..275
NoneNo IPR availableCOILSCoilCoilcoord: 154..202
NoneNo IPR availableCOILSCoilCoilcoord: 387..449
NoneNo IPR availableCOILSCoilCoilcoord: 359..379
NoneNo IPR availableGENE3D1.10.287.1490coord: 316..489
e-value: 1.9E-6
score: 29.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..116
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR32258:SF14TROPOMYOSINcoord: 3..318
coord: 319..492
NoneNo IPR availablePANTHERPTHR32258PROTEIN NETWORKED 4Acoord: 3..318
coord: 319..492

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G028490.1CmaCh04G028490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003779 actin binding