CmaCh04G028320 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G028320
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPentatricopeptide repeat-containing protein
LocationCma_Chr04: 18935929 .. 18937881 (-)
RNA-Seq ExpressionCmaCh04G028320
SyntenyCmaCh04G028320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATACTCATGGAGTCTGCTTGGTTTGCGAGCTCATTTTCGGTGTCAGCTCCTCCTTTCATCCAACTCCTCGCACTTTCAGGTTCTTTCCAATCCGAATCTGCCATCACTTCGCTATTTTTCACTGCTTCATAAGTATCCTGTTCACGACACGAGTATCGTTAATTCTAGATACTGCACGCCTCGAATGCTCTCGTCCGAACCGGAGATTGGACAGAAGGACTCTGCTCACGCTGTTGTGTTTGATATTTTCTCTAAATGTCGGGATGTGGATGAAATTAGGAAAGGCTTAGAGTCGAGTGGTGTTGTTATTAGTCATGATTTGGTGTTGGAGGTGTTGGGGAAGCTTGAGTCGAACCCTGATGAGGCTATAAGGTTTTTCGGTTGGGTTTCGGGGGATTATGGCGAGAAACTTAGCTCCAAGTCGTATAACTTGATGCTTGGAATCCTAGGAGTTAATGGCCGTGTTGAGGAGTTTTGGGATTTGAACTGTGATATCAAGAAAAAGGGTTATGGGATATCTAAAAGTGTACAGAATAAGGTATTGGAGAAGTTTGAGAAGGATGGATTGGAGAGTGAAGCTGAGAGGTTGAGAGACGTCTTTGCATCAGGATCTATTGACAAGTCTCCCGAGAAGATGGGTTCAGTCGTTTGCAAACTTGTTAGGAAAAATGTGTGGGGAAATGATGTTGAGCAGCAATTGCGTGATATGAACGTTTCATTTTCAAGTGATATGGTTAAGATGATATTGGAGAATCTTTGTACAGAACCAGCAAAAGCATTTATATTTTTCCGGTGGATTGGTGAGAGTGGGATGTTTAAGCATGATGAACAGACTTATAATGGCATGGCAAGGGTGTTAGGTAGGGAAGATAGTATCGATAGATTTTGGAAAGTAGTTGATGAAATGAGGAGCCATGGTTACGAAATGGAGGTGGAGACATTTTCTGAGGTGTTGAGACGATTTTGTGCAAGAAGAATGATTGAGGAGGCTGTAAACTTATATGTGTTTGCAATGGCAGGAGGCAATAAGCCTTCGGTTGATTGTCTTACTTTTCTGTTAAAGAAAATAACAGTTAGCAAGCACTTAGATCTTAGTATGTTCTCAAGGGCATTGAAGATATTTACAGAGACAGGCAATGCATTGACGGATTCAATGGTTTTTGCAGTTCTCAAGTCTCTGTCTACTGTTGGTAGGATTGGAGAGTACAACGAGGTTTTAAATGCAATGAAGGAGTATGGATACGTATTTAGTGGCGGTTTGAAGAGAAAGGTAGCATATCAACTTAGTAGTACTGGAAAAAGTGATGAAGCAAATGATTTCGTGAATAGCTTAGAAGCTTCTGGCTGTAATTCAGACAACAAGACCTGGGCAGCTCTGATTGAAGGTTATTGTGTTGCTGGAGATCTTGCTAAAGCTTCTGATTGCATCCACAAAATGTTTGAAAAAGGCGTGGACTGTTGTGCTGGATATGCTTTGGATTTAGTGGTCAATGCTTACTGTCAAAAGAAACGCGAAACTGATGGTAGCCGTCTTCTCTGTGATCTTGTTGATGAAAAGCAGCTAAAACCATGGCATTCTACATATAAAGAATTGATAAACAAGCTATTGGTTCGAGGGGAATTCAGAGAAGCTTTGAAATTGTTGGGGATGATGAGAAATCATGAATTTCCACCATTTATTGACCCCTTTATTTTGTATGTATCAAAGTCTGGAACAGCTGATGATGCCATCGGCTTCCTGAAGGCCATGACATCGAAGAGTTTTCCTTCTACGACAGTATTCCTCCATTTGTTTGAAGCATTTTTCCAAGCTGGAAGGCACGGAGATGCTCAAGACTTCCTTTCAAAATGTCCAGGTTACATTCGTAATCATGCTGATGTTCTGGAGCTTTTTAATTCTACGAGGCTTGTAGAAGCTGCTTCTCCTCCCCCAAATCTGGCTTCTTAG

mRNA sequence

ATGAGATACTCATGGAGTCTGCTTGGTTTGCGAGCTCATTTTCGGTGTCAGCTCCTCCTTTCATCCAACTCCTCGCACTTTCAGGTTCTTTCCAATCCGAATCTGCCATCACTTCGCTATTTTTCACTGCTTCATAAGTATCCTGTTCACGACACGAGTATCGTTAATTCTAGATACTGCACGCCTCGAATGCTCTCGTCCGAACCGGAGATTGGACAGAAGGACTCTGCTCACGCTGTTGTGTTTGATATTTTCTCTAAATGTCGGGATGTGGATGAAATTAGGAAAGGCTTAGAGTCGAGTGGTGTTGTTATTAGTCATGATTTGGTGTTGGAGGTGTTGGGGAAGCTTGAGTCGAACCCTGATGAGGCTATAAGGTTTTTCGGTTGGGTTTCGGGGGATTATGGCGAGAAACTTAGCTCCAAGTCGTATAACTTGATGCTTGGAATCCTAGGAGTTAATGGCCGTGTTGAGGAGTTTTGGGATTTGAACTGTGATATCAAGAAAAAGGGTTATGGGATATCTAAAAGTGTACAGAATAAGGTATTGGAGAAGTTTGAGAAGGATGGATTGGAGAGTGAAGCTGAGAGGTTGAGAGACGTCTTTGCATCAGGATCTATTGACAAGTCTCCCGAGAAGATGGGTTCAGTCGTTTGCAAACTTGTTAGGAAAAATGTGTGGGGAAATGATGTTGAGCAGCAATTGCGTGATATGAACGTTTCATTTTCAAGTGATATGGTTAAGATGATATTGGAGAATCTTTGTACAGAACCAGCAAAAGCATTTATATTTTTCCGGTGGATTGGTGAGAGTGGGATGTTTAAGCATGATGAACAGACTTATAATGGCATGGCAAGGGTGTTAGGTAGGGAAGATAGTATCGATAGATTTTGGAAAGTAGTTGATGAAATGAGGAGCCATGGTTACGAAATGGAGGTGGAGACATTTTCTGAGGTGTTGAGACGATTTTGTGCAAGAAGAATGATTGAGGAGGCTGTAAACTTATATGTGTTTGCAATGGCAGGAGGCAATAAGCCTTCGGTTGATTGTCTTACTTTTCTGTTAAAGAAAATAACAGTTAGCAAGCACTTAGATCTTAGTATGTTCTCAAGGGCATTGAAGATATTTACAGAGACAGGCAATGCATTGACGGATTCAATGGTTTTTGCAGTTCTCAAGTCTCTGTCTACTGTTGGTAGGATTGGAGAGTACAACGAGGTTTTAAATGCAATGAAGGAGTATGGATACGTATTTAGTGGCGGTTTGAAGAGAAAGGTAGCATATCAACTTAGTAGTACTGGAAAAAGTGATGAAGCAAATGATTTCGTGAATAGCTTAGAAGCTTCTGGCTGTAATTCAGACAACAAGACCTGGGCAGCTCTGATTGAAGGTTATTGTGTTGCTGGAGATCTTGCTAAAGCTTCTGATTGCATCCACAAAATGTTTGAAAAAGGCGTGGACTGTTGTGCTGGATATGCTTTGGATTTAGTGGTCAATGCTTACTGTCAAAAGAAACGCGAAACTGATGGTAGCCGTCTTCTCTGTGATCTTGTTGATGAAAAGCAGCTAAAACCATGGCATTCTACATATAAAGAATTGATAAACAAGCTATTGGTTCGAGGGGAATTCAGAGAAGCTTTGAAATTGTTGGGGATGATGAGAAATCATGAATTTCCACCATTTATTGACCCCTTTATTTTGTATGTATCAAAGTCTGGAACAGCTGATGATGCCATCGGCTTCCTGAAGGCCATGACATCGAAGAGTTTTCCTTCTACGACAGTATTCCTCCATTTGTTTGAAGCATTTTTCCAAGCTGGAAGGCACGGAGATGCTCAAGACTTCCTTTCAAAATGTCCAGGTTACATTCGTAATCATGCTGATGTTCTGGAGCTTTTTAATTCTACGAGGCTTGTAGAAGCTGCTTCTCCTCCCCCAAATCTGGCTTCTTAG

Coding sequence (CDS)

ATGAGATACTCATGGAGTCTGCTTGGTTTGCGAGCTCATTTTCGGTGTCAGCTCCTCCTTTCATCCAACTCCTCGCACTTTCAGGTTCTTTCCAATCCGAATCTGCCATCACTTCGCTATTTTTCACTGCTTCATAAGTATCCTGTTCACGACACGAGTATCGTTAATTCTAGATACTGCACGCCTCGAATGCTCTCGTCCGAACCGGAGATTGGACAGAAGGACTCTGCTCACGCTGTTGTGTTTGATATTTTCTCTAAATGTCGGGATGTGGATGAAATTAGGAAAGGCTTAGAGTCGAGTGGTGTTGTTATTAGTCATGATTTGGTGTTGGAGGTGTTGGGGAAGCTTGAGTCGAACCCTGATGAGGCTATAAGGTTTTTCGGTTGGGTTTCGGGGGATTATGGCGAGAAACTTAGCTCCAAGTCGTATAACTTGATGCTTGGAATCCTAGGAGTTAATGGCCGTGTTGAGGAGTTTTGGGATTTGAACTGTGATATCAAGAAAAAGGGTTATGGGATATCTAAAAGTGTACAGAATAAGGTATTGGAGAAGTTTGAGAAGGATGGATTGGAGAGTGAAGCTGAGAGGTTGAGAGACGTCTTTGCATCAGGATCTATTGACAAGTCTCCCGAGAAGATGGGTTCAGTCGTTTGCAAACTTGTTAGGAAAAATGTGTGGGGAAATGATGTTGAGCAGCAATTGCGTGATATGAACGTTTCATTTTCAAGTGATATGGTTAAGATGATATTGGAGAATCTTTGTACAGAACCAGCAAAAGCATTTATATTTTTCCGGTGGATTGGTGAGAGTGGGATGTTTAAGCATGATGAACAGACTTATAATGGCATGGCAAGGGTGTTAGGTAGGGAAGATAGTATCGATAGATTTTGGAAAGTAGTTGATGAAATGAGGAGCCATGGTTACGAAATGGAGGTGGAGACATTTTCTGAGGTGTTGAGACGATTTTGTGCAAGAAGAATGATTGAGGAGGCTGTAAACTTATATGTGTTTGCAATGGCAGGAGGCAATAAGCCTTCGGTTGATTGTCTTACTTTTCTGTTAAAGAAAATAACAGTTAGCAAGCACTTAGATCTTAGTATGTTCTCAAGGGCATTGAAGATATTTACAGAGACAGGCAATGCATTGACGGATTCAATGGTTTTTGCAGTTCTCAAGTCTCTGTCTACTGTTGGTAGGATTGGAGAGTACAACGAGGTTTTAAATGCAATGAAGGAGTATGGATACGTATTTAGTGGCGGTTTGAAGAGAAAGGTAGCATATCAACTTAGTAGTACTGGAAAAAGTGATGAAGCAAATGATTTCGTGAATAGCTTAGAAGCTTCTGGCTGTAATTCAGACAACAAGACCTGGGCAGCTCTGATTGAAGGTTATTGTGTTGCTGGAGATCTTGCTAAAGCTTCTGATTGCATCCACAAAATGTTTGAAAAAGGCGTGGACTGTTGTGCTGGATATGCTTTGGATTTAGTGGTCAATGCTTACTGTCAAAAGAAACGCGAAACTGATGGTAGCCGTCTTCTCTGTGATCTTGTTGATGAAAAGCAGCTAAAACCATGGCATTCTACATATAAAGAATTGATAAACAAGCTATTGGTTCGAGGGGAATTCAGAGAAGCTTTGAAATTGTTGGGGATGATGAGAAATCATGAATTTCCACCATTTATTGACCCCTTTATTTTGTATGTATCAAAGTCTGGAACAGCTGATGATGCCATCGGCTTCCTGAAGGCCATGACATCGAAGAGTTTTCCTTCTACGACAGTATTCCTCCATTTGTTTGAAGCATTTTTCCAAGCTGGAAGGCACGGAGATGCTCAAGACTTCCTTTCAAAATGTCCAGGTTACATTCGTAATCATGCTGATGTTCTGGAGCTTTTTAATTCTACGAGGCTTGTAGAAGCTGCTTCTCCTCCCCCAAATCTGGCTTCTTAG

Protein sequence

MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYCTPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESNPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQNKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNVSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVRGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLFEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS
Homology
BLAST of CmaCh04G028320 vs. ExPASy Swiss-Prot
Match: Q9M891 (Pentatricopeptide repeat-containing protein At3g02490, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g02490 PE=2 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 6.5e-178
Identity = 337/655 (51.45%), Postives = 439/655 (67.02%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC 60
           MRY W  L  R++        S+ S FQV+SN    S R FS      +H+   V  R C
Sbjct: 1   MRYQWRSLLFRSYRSSPRPFLSHHSRFQVISN----STRSFSSF----LHERFGVQQRQC 60

Query: 61  -----------TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDL 120
                        R  SSE  I +K  A  VV D+FS+    DEI K L+S+ VVISH+L
Sbjct: 61  LFALRSPLASSVSRRFSSESAIEEKLPAETVVIDVFSRLNGKDEITKELDSNDVVISHEL 120

Query: 121 VLEVLGKLESNPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKK 180
            L VL +LES+PD A RFF W    Y +KLSSKSYN ML I GVNG V+EFW L  D+KK
Sbjct: 121 ALRVLRELESSPDVAGRFFKWGLEAYPQKLSSKSYNTMLRIFGVNGLVDEFWRLVDDMKK 180

Query: 181 KGYGISKSVQNKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGN 240
           KG+G+S +V+++V +KF+KDGLE++ ERL+++FASGS+D S +K+ + VCK+V K VWG 
Sbjct: 181 KGHGVSANVRDRVGDKFKKDGLENDLERLKELFASGSMDNSVDKVCNRVCKIVMKEVWGA 240

Query: 241 DVEQQLRDMNVSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLG 300
           DVE+QLRD+ + F SD+VKM+LE L  +P KA +FFRWI ESG FKHDE+TYN MARVLG
Sbjct: 241 DVEKQLRDLKLEFKSDVVKMVLEKLDVDPRKALLFFRWIDESGSFKHDEKTYNAMARVLG 300

Query: 301 REDSIDRFWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAG--GNKPS 360
           +E  +DRF  +++E+RS GYEME+ET+  V  RFC  +MI+EAV L+ FAMAG   N P+
Sbjct: 301 KEKFLDRFQHMIEEIRSAGYEMEMETYVRVSARFCQTKMIKEAVELFEFAMAGSISNTPT 360

Query: 361 VDCLTFLLKKITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEV 420
             C + LLKKI  +K LD+ +F+R LK +T  GN + D M+  VLKSL +V R G+ NEV
Sbjct: 361 PHCCSLLLKKIVTAKKLDMDLFTRTLKAYTGNGNVVPDVMLQHVLKSLRSVDRFGQSNEV 420

Query: 421 LNAMKEYGYVFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCV 480
           L AM E GYV SG L+  +A  LS  GK DEAN+ VN +EASG + D+K  A+L+EG+C 
Sbjct: 421 LKAMNEGGYVPSGDLQSVIASGLSRKGKKDEANELVNFMEASGNHLDDKAMASLVEGHCD 480

Query: 481 AGDLAKASDCIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWH 540
           A DL +AS+C  KM  K     AGYA + +V AYC   +  D  +L  +LV + QLKPWH
Sbjct: 481 AKDLEEASECFKKMIGKEGVSYAGYAFEKLVLAYCNSFQARDVYKLFSELVKQNQLKPWH 540

Query: 541 STYKELINKLLVR-----GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFL 600
           STYK ++  LL++     G F EAL LL MMRNH FPPF+DPF+ Y+S SGT+ +A  FL
Sbjct: 541 STYKIMVRNLLMKKVARDGGFEEALSLLPMMRNHGFPPFVDPFMDYLSNSGTSAEAFAFL 600

Query: 601 KAMTSKSFPSTTVFLHLFEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTR 638
           KA+TSK FPS ++ L +FEA  ++ RH +AQD LS  P YIR +A+VLELFN+ +
Sbjct: 601 KAVTSKKFPSNSMVLRVFEAMLKSARHSEAQDLLSMSPSYIRRNAEVLELFNTMK 647

BLAST of CmaCh04G028320 vs. ExPASy Swiss-Prot
Match: Q8LPF1 (Pentatricopeptide repeat-containing protein At5g15980, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g15980 PE=2 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 8.8e-175
Identity = 334/667 (50.07%), Postives = 441/667 (66.12%), Query Frame = 0

Query: 1   MRY-SWSLLGLRAHFR--------CQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHD 60
           MRY  W L+ LR++ R        C  + S +S  F    +P + +L+    L       
Sbjct: 1   MRYQQWRLMLLRSYHRSHLPYLSPCSQVTSISSRSFSSFIHPGIGALQQSEQLCPLRSPM 60

Query: 61  TSIVNSRYCTPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVL 120
           TS  N      R  SSEP + +K SA A V DIFS+    DEIRK LESSGVVIS DL L
Sbjct: 61  TSSGNLVKSVGRSFSSEPAVEEKSSAEATVIDIFSRLSGEDEIRKELESSGVVISQDLAL 120

Query: 121 EVLGKLESNPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKG 180
           +VL KLESNPD A  FF W+     E+LSSK+YN+ML ILG NG V+EFW L   +KKKG
Sbjct: 121 KVLRKLESNPDVAKSFFQWIKEASPEELSSKNYNMMLRILGGNGLVDEFWGLVDVMKKKG 180

Query: 181 YGISKSVQNKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDV 240
           +G+S +V++KV +KF+KDGLES+  RLR +F S  +D S E +   VCK+V K  WG+DV
Sbjct: 181 HGLSANVRDKVGDKFQKDGLESDLLRLRKLFTSDCLDNSAENVCDRVCKIVMKEEWGDDV 240

Query: 241 EQQLRDMNVSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGRE 300
           E+++RD+NV F SD+VKMI+E L  EP KA +FFRWI ES +FKHDE+TYN MARVLG+E
Sbjct: 241 EKRVRDLNVEFKSDLVKMIVERLDVEPRKALLFFRWIDESDLFKHDEKTYNAMARVLGKE 300

Query: 301 DSIDRFWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAG---GNKPSV 360
             +DRF  +V EMRS GYE+E+ET+  V  RFC  ++I+EAV+L+  AMAG    N P+ 
Sbjct: 301 KFLDRFQNIVVEMRSAGYEVEIETYVRVSTRFCQTKLIKEAVDLFEIAMAGSSSSNNPTP 360

Query: 361 DCLTFLLKKITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVL 420
            C   LLKKI  +K LD+ +FSRA+K++T+ GNALTDS++ +VLKSL +V R+ + NE+L
Sbjct: 361 HCFCLLLKKIVTAKILDMDLFSRAVKVYTKNGNALTDSLLKSVLKSLRSVDRVEQSNELL 420

Query: 421 NAMKEYGYVFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVA 480
             MK  GYV SG ++  +A  LS  GK DEA++FV+ +E+SG N D+K  A+L+EGYC +
Sbjct: 421 KEMKRGGYVPSGDMQSMIASSLSRKGKKDEADEFVDFMESSGNNLDDKAMASLVEGYCDS 480

Query: 481 GDLAKASDCIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHS 540
           G+L +A  C  KM        A Y+ + +V AYC K +  D  +LL   V + QLKP HS
Sbjct: 481 GNLDEALVCFEKMVGNTGVSYADYSFEKLVLAYCNKNQVRDAYKLLSAQVTKNQLKPRHS 540

Query: 541 TYKELINKLLVR-----GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLK 600
           TYK L+  LL +     G F EAL LL +M++H FPPFIDPF+ Y S +G + +A+GFLK
Sbjct: 541 TYKSLVTNLLTKKIARDGGFEEALSLLPIMKDHGFPPFIDPFMSYFSSTGKSTEALGFLK 600

Query: 601 AMTSKSFPSTTVFLHLFEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAAS 651
           AMTS +FP  +V L +FE   ++ RH +AQD LS CP YIRN+ DVLELFN+ +  E+A 
Sbjct: 601 AMTSNNFPYISVVLRVFETMMKSARHSEAQDLLSLCPNYIRNNPDVLELFNTMKPNESAV 660

BLAST of CmaCh04G028320 vs. ExPASy Swiss-Prot
Match: Q9STK5 (Pentatricopeptide repeat-containing protein At3g48250, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g48250 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.0e-90
Identity = 202/639 (31.61%), Postives = 333/639 (52.11%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC 60
           M  S ++L    H   Q+   S  S  +V  + NL S          P+   +IV SR+ 
Sbjct: 1   MYRSMAILSSLRHAYSQISTRSYLSRSKVGFSSNLSS----------PLDSFAIVPSRFL 60

Query: 61  TP-RMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120
              R  SS+P+         V+ + +SK     E+ +GL    + ++H+  + VL KLE 
Sbjct: 61  WKFRTFSSKPD----SMLQLVLENDWSK-----EVEEGLRKPDMSLTHETAIYVLRKLEK 120

Query: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQ 180
            P++A  F  WV  D G   S+  Y++ML IL     ++ FW    ++K+ G+ + +   
Sbjct: 121 YPEKAYYFLDWVLRDSGLSPSTPLYSIMLRILVQQRSMKRFWMTLREMKQGGFYLDEDTY 180

Query: 181 NKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMN 240
             +  +  K+  +++A  +   +     + +   +   V  +V K  W  +VE++L++M 
Sbjct: 181 KTIYGELSKEKSKADAVAVAHFYERMLKENAMSVVAGEVSAVVTKGDWSCEVERELQEMK 240

Query: 241 VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGM---FKHDEQTYNGMARVLGREDSIDR 300
           +  S + V  +L+ L   P KA  FF W+G  G    ++H   TYN   RVL R +S+  
Sbjct: 241 LVLSDNFVIRVLKELREHPLKALAFFHWVGGGGSSSGYQHSTVTYNAALRVLARPNSVAE 300

Query: 301 FWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLK 360
           FW VVDEM++ GY+M+++T+ +V R+F   RM+ E V LY + M G  KPS+   + LL+
Sbjct: 301 FWSVVDEMKTAGYDMDLDTYIKVSRQFQKSRMMAETVKLYEYMMDGPFKPSIQDCSLLLR 360

Query: 361 KITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGY 420
            ++ S + DL +  R  + +  TG +L+ ++   + +SL++VGR  E  E+  AM+  GY
Sbjct: 361 YLSGSPNPDLDLVFRVSRKYESTGKSLSKAVYDGIHRSLTSVGRFDEAEEITKAMRNAGY 420

Query: 421 VFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASD 480
                   ++ + L    + +EA   ++ +EA GC  D KTW  LI+G+C   +L KA  
Sbjct: 421 EPDNITYSQLVFGLCKAKRLEEARGVLDQMEAQGCFPDIKTWTILIQGHCKNNELDKALA 480

Query: 481 CIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINK 540
           C   M EKG D  +   LD++++ +    +    S  L ++V    +KPW STYK LI+K
Sbjct: 481 CFANMLEKGFDIDSN-LLDVLIDGFVIHNKFEGASIFLMEMVKNANVKPWQSTYKLLIDK 540

Query: 541 LLVRGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVF 600
           LL   +  EAL LL MM+   +P + + F  Y++K GT +DA  FL  ++SK  PS   +
Sbjct: 541 LLKIKKSEEALDLLQMMKKQNYPAYAEAFDGYLAKFGTLEDAKKFLDVLSSKDSPSFAAY 600

Query: 601 LHLFEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNS 636
            H+ EAF++ GR  DA++ L  CP + + H  + ELF +
Sbjct: 601 FHVIEAFYREGRLTDAKNLLFICPHHFKTHPKISELFGA 619

BLAST of CmaCh04G028320 vs. ExPASy Swiss-Prot
Match: Q9FMF6 (Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g64320 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 4.4e-17
Identity = 117/532 (21.99%), Postives = 214/532 (40.23%), Query Frame = 0

Query: 67  SEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESNPDEAIR 126
           S PEIG  DSA+   ++   K  D+D +R           + L+     +L  N   ++ 
Sbjct: 44  SSPEIGGTDSANE--WEKLLKPFDLDSLRNSFHKITPFQLYKLL-----ELPLNVSTSME 103

Query: 127 FFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQNKVLEKF 186
            F W     G + S   Y +++G LG NG  +    L   +K +G    +S+   ++  +
Sbjct: 104 LFSWTGSQNGYRHSFDVYQVLIGKLGANGEFKTIDRLLIQMKDEGIVFKESLFISIMRDY 163

Query: 187 EKDGLESEAERL----RDVFASGSIDKS----PEKMGSVVCKLVRKNVWGNDVEQQLRDM 246
           +K G   +  RL    R+V++     KS     E + S  C  V  NV+ + + +++   
Sbjct: 164 DKAGFPGQTTRLMLEMRNVYSCEPTFKSYNVVLEILVSGNCHKVAANVFYDMLSRKIPPT 223

Query: 247 NVSFSSDMVKMILENLC--TEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDR 306
             +F      ++++  C   E   A    R + + G    +   Y  +   L + + ++ 
Sbjct: 224 LFTFG-----VVMKAFCAVNEIDSALSLLRDMTKHGCVP-NSVIYQTLIHSLSKCNRVNE 283

Query: 307 FWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLK 366
             ++++EM   G   + ETF++V+   C    I EA  +    +  G  P      +L+ 
Sbjct: 284 ALQLLEEMFLMGCVPDAETFNDVILGLCKFDRINEAAKMVNRMLIRGFAPDDITYGYLMN 343

Query: 367 ------KITVSKHL-------DLSMFSRALKIFTETGNALTDSMVFA-VLKSLSTVGRIG 426
                 ++  +K L       ++ +F+  +  F   G       V + ++ S   V  + 
Sbjct: 344 GLCKIGRVDAAKDLFYRIPKPEIVIFNTLIHGFVTHGRLDDAKAVLSDMVTSYGIVPDVC 403

Query: 427 EYNEVLNAMKEYGYVFSG--GLKRKVAYQLSS-------------------TGKSDEAND 486
            YN ++     YGY   G  GL  +V + + +                    GK DEA +
Sbjct: 404 TYNSLI-----YGYWKEGLVGLALEVLHDMRNKGCKPNVYSYTILVDGFCKLGKIDEAYN 463

Query: 487 FVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHKMFEKGVDCCAGYALDLVVNAY 546
            +N + A G   +   +  LI  +C    + +A +   +M  KG      Y  + +++  
Sbjct: 464 VLNEMSADGLKPNTVGFNCLISAFCKEHRIPEAVEIFREMPRKGCKPDV-YTFNSLISGL 523

Query: 547 CQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVRGEFREALKLLGMM 554
           C+         LL D++ E  +     TY  LIN  L RGE +EA KL+  M
Sbjct: 524 CEVDEIKHALWLLRDMISEGVVAN-TVTYNTLINAFLRRGEIKEARKLVNEM 555

BLAST of CmaCh04G028320 vs. ExPASy Swiss-Prot
Match: Q9LSL9 (Pentatricopeptide repeat-containing protein At5g65560 OS=Arabidopsis thaliana OX=3702 GN=At5g65560 PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 6.0e-14
Identity = 112/504 (22.22%), Postives = 206/504 (40.87%), Query Frame = 0

Query: 114 LGKLESNPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYG 173
           L  L+ +P  A+ F  W+S +   K S  SY  +L +L  NG V   + +   + K    
Sbjct: 96  LFSLDLDPKTALNFSHWISQNPRYKHSVYSYASLLTLLINNGYVGVVFKIRLLMIKSCDS 155

Query: 174 ISKSVQ-NKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVE 233
           +  ++    +  K  KD    E   L+     G  +     +         K V+   +E
Sbjct: 156 VGDALYVLDLCRKMNKD----ERFELKYKLIIGCYNTLLNSLARFGLVDEMKQVYMEMLE 215

Query: 234 QQLRDMNVSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGRED 293
            ++   N+   + MV    +    E A  ++    I E+G+   D  TY  +     +  
Sbjct: 216 DKVCP-NIYTYNKMVNGYCKLGNVEEANQYV--SKIVEAGL-DPDFFTYTSLIMGYCQRK 275

Query: 294 SIDRFWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLT 353
            +D  +KV +EM   G       ++ ++   C  R I+EA++L+V        P+V   T
Sbjct: 276 DLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYT 335

Query: 354 FLLKKITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMK 413
            L+K +  S+    ++    +K   ETG          ++ SL +  +  +  E+L  M 
Sbjct: 336 VLIKSLCGSERKSEAL--NLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQML 395

Query: 414 EYGYVFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLA 473
           E G + +      +       G  ++A D V  +E+   + + +T+  LI+GYC   ++ 
Sbjct: 396 EKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYC-KSNVH 455

Query: 474 KASDCIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKE 533
           KA   ++KM E+ V        + +++  C+        RLL  L++++ L P   TY  
Sbjct: 456 KAMGVLNKMLERKV-LPDVVTYNSLIDGQCRSGNFDSAYRLL-SLMNDRGLVPDQWTYTS 515

Query: 534 LINKLLVRGEFREALKLLGMMRNHEFPPFIDPFILYVS---KSGTADDAIGFLKAMTSKS 593
           +I+ L       EA  L   +      P +  +   +    K+G  D+A   L+ M SK+
Sbjct: 516 MIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKN 575

Query: 594 -FPSTTVFLHLFEAFFQAGRHGDA 613
             P++  F  L       G+  +A
Sbjct: 576 CLPNSLTFNALIHGLCADGKLKEA 586

BLAST of CmaCh04G028320 vs. ExPASy TrEMBL
Match: A0A6J1JSU2 (pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487528 PE=4 SV=1)

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 650/650 (100.00%), Postives = 650/650 (100.00%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC 60
           MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC
Sbjct: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC 60

Query: 61  TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESN 120
           TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESN
Sbjct: 61  TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESN 120

Query: 121 PDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQN 180
           PDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQN
Sbjct: 121 PDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQN 180

Query: 181 KVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNV 240
           KVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNV
Sbjct: 181 KVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNV 240

Query: 241 SFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKV 300
           SFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKV
Sbjct: 241 SFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKV 300

Query: 301 VDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITV 360
           VDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITV
Sbjct: 301 VDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITV 360

Query: 361 SKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSG 420
           SKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSG
Sbjct: 361 SKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSG 420

Query: 421 GLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHK 480
           GLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHK
Sbjct: 421 GLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHK 480

Query: 481 MFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVR 540
           MFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVR
Sbjct: 481 MFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVR 540

Query: 541 GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLF 600
           GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLF
Sbjct: 541 GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLF 600

Query: 601 EAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 651
           EAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS
Sbjct: 601 EAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 650

BLAST of CmaCh04G028320 vs. ExPASy TrEMBL
Match: A0A6J1E5F8 (pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429656 PE=4 SV=1)

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 624/651 (95.85%), Postives = 634/651 (97.39%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYF-SLLHKYPVHDTSIVNSRY 60
           MRYSW LL LRAHFRCQLLLSSNSSHFQVLSNPNL SLRYF SLL KYPVHDTSIVNSRY
Sbjct: 1   MRYSWRLLRLRAHFRCQLLLSSNSSHFQVLSNPNLQSLRYFSSLLRKYPVHDTSIVNSRY 60

Query: 61  CTPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120
            TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES
Sbjct: 61  STPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120

Query: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQ 180
           NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCD+KKKGYGISKSVQ
Sbjct: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDMKKKGYGISKSVQ 180

Query: 181 NKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMN 240
           NKVLEKFEKDGLESEAE+LRDVFASGSIDKSPEK+GS+VCKLVRKNVWG+DVEQQLRDMN
Sbjct: 181 NKVLEKFEKDGLESEAEKLRDVFASGSIDKSPEKIGSIVCKLVRKNVWGDDVEQQLRDMN 240

Query: 241 VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWK 300
           +S SSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYN MARVLGREDSIDRFWK
Sbjct: 241 ISLSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNAMARVLGREDSIDRFWK 300

Query: 301 VVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIT 360
           VVDEMRSHGYEMEVETFSEVLRRFC R+MIEEAVNLYVFAMAGGNKPSVDCLTFLLKKI 
Sbjct: 301 VVDEMRSHGYEMEVETFSEVLRRFCKRKMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIA 360

Query: 361 VSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420
           VSKHLDLS+FSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS
Sbjct: 361 VSKHLDLSLFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420

Query: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480
           GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH
Sbjct: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480

Query: 481 KMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLV 540
           KM EKGVDCCAGY LDLVVNAYCQKKRETD SRL CDLVDEKQLKPWHSTYK LINKLLV
Sbjct: 481 KMVEKGVDCCAGYTLDLVVNAYCQKKRETDASRLFCDLVDEKQLKPWHSTYKALINKLLV 540

Query: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600
           RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL
Sbjct: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600

Query: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 651
           FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNS + VEAA+PPPNLAS
Sbjct: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSMKHVEAAAPPPNLAS 651

BLAST of CmaCh04G028320 vs. ExPASy TrEMBL
Match: A0A6J1JQW6 (pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487528 PE=4 SV=1)

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 64  MLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESNPDE 123
           MLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESNPDE
Sbjct: 1   MLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESNPDE 60

Query: 124 AIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQNKVL 183
           AIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQNKVL
Sbjct: 61  AIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQNKVL 120

Query: 184 EKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNVSFS 243
           EKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNVSFS
Sbjct: 121 EKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNVSFS 180

Query: 244 SDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKVVDE 303
           SDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKVVDE
Sbjct: 181 SDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKVVDE 240

Query: 304 MRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITVSKH 363
           MRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITVSKH
Sbjct: 241 MRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITVSKH 300

Query: 364 LDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSGGLK 423
           LDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSGGLK
Sbjct: 301 LDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSGGLK 360

Query: 424 RKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHKMFE 483
           RKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHKMFE
Sbjct: 361 RKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHKMFE 420

Query: 484 KGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVRGEF 543
           KGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVRGEF
Sbjct: 421 KGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVRGEF 480

Query: 544 REALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLFEAF 603
           REALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLFEAF
Sbjct: 481 REALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLFEAF 540

Query: 604 FQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 651
           FQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS
Sbjct: 541 FQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 587

BLAST of CmaCh04G028320 vs. ExPASy TrEMBL
Match: A0A6J1E0A6 (pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429656 PE=4 SV=1)

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 566/587 (96.42%), Postives = 576/587 (98.13%), Query Frame = 0

Query: 64  MLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESNPDE 123
           MLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESNPDE
Sbjct: 1   MLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESNPDE 60

Query: 124 AIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQNKVL 183
           AIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCD+KKKGYGISKSVQNKVL
Sbjct: 61  AIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDMKKKGYGISKSVQNKVL 120

Query: 184 EKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNVSFS 243
           EKFEKDGLESEAE+LRDVFASGSIDKSPEK+GS+VCKLVRKNVWG+DVEQQLRDMN+S S
Sbjct: 121 EKFEKDGLESEAEKLRDVFASGSIDKSPEKIGSIVCKLVRKNVWGDDVEQQLRDMNISLS 180

Query: 244 SDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKVVDE 303
           SDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYN MARVLGREDSIDRFWKVVDE
Sbjct: 181 SDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNAMARVLGREDSIDRFWKVVDE 240

Query: 304 MRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITVSKH 363
           MRSHGYEMEVETFSEVLRRFC R+MIEEAVNLYVFAMAGGNKPSVDCLTFLLKKI VSKH
Sbjct: 241 MRSHGYEMEVETFSEVLRRFCKRKMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIAVSKH 300

Query: 364 LDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSGGLK 423
           LDLS+FSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSGGLK
Sbjct: 301 LDLSLFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSGGLK 360

Query: 424 RKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHKMFE 483
           RKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHKM E
Sbjct: 361 RKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHKMVE 420

Query: 484 KGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVRGEF 543
           KGVDCCAGY LDLVVNAYCQKKRETD SRL CDLVDEKQLKPWHSTYK LINKLLVRGEF
Sbjct: 421 KGVDCCAGYTLDLVVNAYCQKKRETDASRLFCDLVDEKQLKPWHSTYKALINKLLVRGEF 480

Query: 544 REALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLFEAF 603
           REALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLFEAF
Sbjct: 481 REALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLFEAF 540

Query: 604 FQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 651
           FQAGRHGDAQDFLSKCPGYIRNHADVLELFNS + VEAA+PPPNLAS
Sbjct: 541 FQAGRHGDAQDFLSKCPGYIRNHADVLELFNSMKHVEAAAPPPNLAS 587

BLAST of CmaCh04G028320 vs. ExPASy TrEMBL
Match: A0A6J1FJW5 (pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111444701 PE=4 SV=1)

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 550/662 (83.08%), Postives = 582/662 (87.92%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYF-SLLHKYPVHD-------- 60
           MRYSW LL LRAHFR QL +SSN+SHFQV S+PNL SLR+  SLL K+PVH         
Sbjct: 1   MRYSWRLLRLRAHFRSQLRISSNASHFQVHSDPNLQSLRFLSSLLPKHPVHGTASPPISD 60

Query: 61  ---TSIVNSRYCTPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHD 120
              TSIV SRY TPR  SSE  I QK+S HAVVFDIFSK RDVDEIRK LES+G+VISHD
Sbjct: 61  SQCTSIVKSRYGTPRTFSSESVIEQKESDHAVVFDIFSKSRDVDEIRKDLESNGIVISHD 120

Query: 121 LVLEVLGKLESNPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIK 180
           LVLEVLGKLESNPD AIRFF WVSGDYGEKLSSKSYNLMLGILGVN  V EFWDLNCD+K
Sbjct: 121 LVLEVLGKLESNPDSAIRFFNWVSGDYGEKLSSKSYNLMLGILGVNDHVGEFWDLNCDMK 180

Query: 181 KKGYGISKSVQNKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWG 240
           KKGYGISK+V++KVLEKFEKDGLESEAE+LRDVFASGS DKSPEK+GS+VCKLVR NVWG
Sbjct: 181 KKGYGISKTVRDKVLEKFEKDGLESEAEKLRDVFASGSTDKSPEKIGSIVCKLVRNNVWG 240

Query: 241 NDVEQQLRDMNVSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVL 300
           +DVEQQL DMNVSFSSDMVKMILENLCT+PAKAFIFFRWIGESGMFKHDEQTYN MARVL
Sbjct: 241 DDVEQQLCDMNVSFSSDMVKMILENLCTDPAKAFIFFRWIGESGMFKHDEQTYNAMARVL 300

Query: 301 GREDSIDRFWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSV 360
           GRED IDRFWKVVDEMRSHGYEM VETF++VL RFC RRMIEEAVNLYVFAMAGGNKPSV
Sbjct: 301 GREDCIDRFWKVVDEMRSHGYEMGVETFAKVLGRFCKRRMIEEAVNLYVFAMAGGNKPSV 360

Query: 361 DCLTFLLKKITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVL 420
           DCLTFLLKKI VSKHLDL +FSR LK+FTETGN LTDSMV AVLKSLSTVGRIGEY EVL
Sbjct: 361 DCLTFLLKKIAVSKHLDLDLFSRTLKVFTETGNVLTDSMVSAVLKSLSTVGRIGEYTEVL 420

Query: 421 NAMKEYGYVFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVA 480
           N MKEYGY FSG LKRKVAY+LS TGKSDEANDF+N LEASGCN DNKTWA+LIEG C A
Sbjct: 421 NVMKEYGYEFSGSLKRKVAYRLSRTGKSDEANDFMNGLEASGCNPDNKTWASLIEGLCAA 480

Query: 481 GDLAKASDCIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHS 540
           GDLA ASDCIHKM EKG    AGYALDL++NAYCQK+RETD S LL DLVD KQLKPWHS
Sbjct: 481 GDLAMASDCIHKMVEKGDVSNAGYALDLIINAYCQKRRETDASHLLSDLVDGKQLKPWHS 540

Query: 541 TYKELINKLLVRGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSK 600
           TYK LINKLL RGEF+EALKLLGMMRNHEFPPFI+PFI YVSKSGTADDAIGFLKAMTSK
Sbjct: 541 TYKALINKLLQRGEFKEALKLLGMMRNHEFPPFIEPFISYVSKSGTADDAIGFLKAMTSK 600

Query: 601 SFPSTTVFLHLFEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNL 651
            FPST+VFLHLFEAFFQAGRHGDAQDFLSKCP  IRNHADVL LF S + VEAA+  PNL
Sbjct: 601 RFPSTSVFLHLFEAFFQAGRHGDAQDFLSKCPSDIRNHADVLNLFYSMKPVEAAA-SPNL 660

BLAST of CmaCh04G028320 vs. NCBI nr
Match: XP_022990729.1 (pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 650/650 (100.00%), Postives = 650/650 (100.00%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC 60
           MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC
Sbjct: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC 60

Query: 61  TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESN 120
           TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESN
Sbjct: 61  TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESN 120

Query: 121 PDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQN 180
           PDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQN
Sbjct: 121 PDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQN 180

Query: 181 KVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNV 240
           KVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNV
Sbjct: 181 KVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMNV 240

Query: 241 SFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKV 300
           SFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKV
Sbjct: 241 SFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWKV 300

Query: 301 VDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITV 360
           VDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITV
Sbjct: 301 VDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKITV 360

Query: 361 SKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSG 420
           SKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSG
Sbjct: 361 SKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFSG 420

Query: 421 GLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHK 480
           GLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHK
Sbjct: 421 GLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHK 480

Query: 481 MFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVR 540
           MFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVR
Sbjct: 481 MFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVR 540

Query: 541 GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLF 600
           GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLF
Sbjct: 541 GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHLF 600

Query: 601 EAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 651
           EAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS
Sbjct: 601 EAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 650

BLAST of CmaCh04G028320 vs. NCBI nr
Match: XP_022921370.1 (pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 624/651 (95.85%), Postives = 634/651 (97.39%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYF-SLLHKYPVHDTSIVNSRY 60
           MRYSW LL LRAHFRCQLLLSSNSSHFQVLSNPNL SLRYF SLL KYPVHDTSIVNSRY
Sbjct: 1   MRYSWRLLRLRAHFRCQLLLSSNSSHFQVLSNPNLQSLRYFSSLLRKYPVHDTSIVNSRY 60

Query: 61  CTPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120
            TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES
Sbjct: 61  STPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120

Query: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQ 180
           NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCD+KKKGYGISKSVQ
Sbjct: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDMKKKGYGISKSVQ 180

Query: 181 NKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMN 240
           NKVLEKFEKDGLESEAE+LRDVFASGSIDKSPEK+GS+VCKLVRKNVWG+DVEQQLRDMN
Sbjct: 181 NKVLEKFEKDGLESEAEKLRDVFASGSIDKSPEKIGSIVCKLVRKNVWGDDVEQQLRDMN 240

Query: 241 VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWK 300
           +S SSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYN MARVLGREDSIDRFWK
Sbjct: 241 ISLSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNAMARVLGREDSIDRFWK 300

Query: 301 VVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIT 360
           VVDEMRSHGYEMEVETFSEVLRRFC R+MIEEAVNLYVFAMAGGNKPSVDCLTFLLKKI 
Sbjct: 301 VVDEMRSHGYEMEVETFSEVLRRFCKRKMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIA 360

Query: 361 VSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420
           VSKHLDLS+FSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS
Sbjct: 361 VSKHLDLSLFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420

Query: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480
           GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH
Sbjct: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480

Query: 481 KMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLV 540
           KM EKGVDCCAGY LDLVVNAYCQKKRETD SRL CDLVDEKQLKPWHSTYK LINKLLV
Sbjct: 481 KMVEKGVDCCAGYTLDLVVNAYCQKKRETDASRLFCDLVDEKQLKPWHSTYKALINKLLV 540

Query: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600
           RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL
Sbjct: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600

Query: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 651
           FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNS + VEAA+PPPNLAS
Sbjct: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSMKHVEAAAPPPNLAS 651

BLAST of CmaCh04G028320 vs. NCBI nr
Match: KAG6602554.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1249.6 bits (3232), Expect = 0.0e+00
Identity = 623/651 (95.70%), Postives = 631/651 (96.93%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYF-SLLHKYPVHDTSIVNSRY 60
           MRYSW LL LRAHFRCQLLLSSNSSHFQVLSNPNL SLRYF SLLHKY VH TSIVNSRY
Sbjct: 1   MRYSWRLLRLRAHFRCQLLLSSNSSHFQVLSNPNLQSLRYFSSLLHKYSVHGTSIVNSRY 60

Query: 61  CTPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120
            TPRMLS EPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES
Sbjct: 61  RTPRMLSYEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120

Query: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQ 180
           NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCD+KKKGYGISKSVQ
Sbjct: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDMKKKGYGISKSVQ 180

Query: 181 NKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMN 240
           NKVLEKFEKDGLESEAE+LRDVFASGSIDKSPEK+GS+VCKLVRKNVWG+DVEQQLRDMN
Sbjct: 181 NKVLEKFEKDGLESEAEKLRDVFASGSIDKSPEKIGSIVCKLVRKNVWGDDVEQQLRDMN 240

Query: 241 VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWK 300
           VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYN MARVLG EDSIDRFWK
Sbjct: 241 VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNAMARVLGSEDSIDRFWK 300

Query: 301 VVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIT 360
           VVDEMRSHGYEMEVETFSEVLRRFC RRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKI 
Sbjct: 301 VVDEMRSHGYEMEVETFSEVLRRFCKRRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIA 360

Query: 361 VSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420
           VSKHLDLS+FSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS
Sbjct: 361 VSKHLDLSLFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420

Query: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480
           GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH
Sbjct: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480

Query: 481 KMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLV 540
           KM EKGVDCCAGY LDLVVNAYCQKKRETD SRL CDLVDEKQLKPW STYK LINKLLV
Sbjct: 481 KMVEKGVDCCAGYTLDLVVNAYCQKKRETDASRLFCDLVDEKQLKPWDSTYKALINKLLV 540

Query: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600
           RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL
Sbjct: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600

Query: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 651
           FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNS + VEAA+PPPNLAS
Sbjct: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSMKHVEAAAPPPNLAS 651

BLAST of CmaCh04G028320 vs. NCBI nr
Match: XP_023511958.1 (pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1248.4 bits (3229), Expect = 0.0e+00
Identity = 619/651 (95.08%), Postives = 632/651 (97.08%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYF-SLLHKYPVHDTSIVNSRY 60
           MRYSW LL LRAHFRCQLLLSSNSSHFQVLSNPNL SLRYF SLLHKYPVHDTSIVN RY
Sbjct: 1   MRYSWRLLRLRAHFRCQLLLSSNSSHFQVLSNPNLQSLRYFSSLLHKYPVHDTSIVNFRY 60

Query: 61  CTPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120
            TPRMLS E EIGQKDSAHAV+FDIFSKCRDVDEIRKGLESSG+VISHDLVLEVLGKLES
Sbjct: 61  RTPRMLSYESEIGQKDSAHAVLFDIFSKCRDVDEIRKGLESSGIVISHDLVLEVLGKLES 120

Query: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQ 180
           NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCD+KKKGYGISKSVQ
Sbjct: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDMKKKGYGISKSVQ 180

Query: 181 NKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMN 240
           +KVLEKFEKDGLESEAE+LRDVFASGSIDKSPEK+GS+VCKLVRKNVWG+DVEQQLRDMN
Sbjct: 181 DKVLEKFEKDGLESEAEKLRDVFASGSIDKSPEKIGSIVCKLVRKNVWGDDVEQQLRDMN 240

Query: 241 VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWK 300
           +SFSSDMVKMILENLCTEPAKAFIFFRWIGE+GMFKHDEQTYN MARVLGREDSIDRFWK
Sbjct: 241 ISFSSDMVKMILENLCTEPAKAFIFFRWIGENGMFKHDEQTYNAMARVLGREDSIDRFWK 300

Query: 301 VVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIT 360
           VVDEMRSHGYE+EVETFSEVLRRFC RRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKI 
Sbjct: 301 VVDEMRSHGYEIEVETFSEVLRRFCKRRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIA 360

Query: 361 VSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420
           VSKHLDLS+FSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS
Sbjct: 361 VSKHLDLSLFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420

Query: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480
           GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH
Sbjct: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480

Query: 481 KMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLV 540
           KM EKGVDCCAGY LDLVVNAYCQKKRETD SRL CDLVDEKQLKPWHSTYK LINKLLV
Sbjct: 481 KMVEKGVDCCAGYTLDLVVNAYCQKKRETDASRLFCDLVDEKQLKPWHSTYKALINKLLV 540

Query: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600
           RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL
Sbjct: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600

Query: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 651
           FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNS + VEAA PPPNLAS
Sbjct: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSMKHVEAAPPPPNLAS 651

BLAST of CmaCh04G028320 vs. NCBI nr
Match: KAG7033234.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 622/651 (95.55%), Postives = 631/651 (96.93%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYF-SLLHKYPVHDTSIVNSRY 60
           MRYSW LL LRAHFRCQLLLSSNSSHFQVLSNPNL SLRYF SLLHKY VH TSIVNSRY
Sbjct: 1   MRYSWRLLRLRAHFRCQLLLSSNSSHFQVLSNPNLQSLRYFSSLLHKYSVHGTSIVNSRY 60

Query: 61  CTPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120
            TPRMLS EPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES
Sbjct: 61  RTPRMLSYEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120

Query: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQ 180
           NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCD+KKKGYGISKSVQ
Sbjct: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDMKKKGYGISKSVQ 180

Query: 181 NKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMN 240
           NKVLEKFEKDGLESEAE+LRDVFASGSIDKSPEK+GS+VCKLVRKNVWG+DVEQQLRDMN
Sbjct: 181 NKVLEKFEKDGLESEAEKLRDVFASGSIDKSPEKIGSIVCKLVRKNVWGDDVEQQLRDMN 240

Query: 241 VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDRFWK 300
           VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYN MARVLG EDSI+RFWK
Sbjct: 241 VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNAMARVLGSEDSIERFWK 300

Query: 301 VVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIT 360
           VVDEMRSHGYEMEVETFSEVLRRFC RRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKI 
Sbjct: 301 VVDEMRSHGYEMEVETFSEVLRRFCKRRMIEEAVNLYVFAMAGGNKPSVDCLTFLLKKIA 360

Query: 361 VSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420
           VSKHLDLS+FSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS
Sbjct: 361 VSKHLDLSLFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGYVFS 420

Query: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480
           GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH
Sbjct: 421 GGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIH 480

Query: 481 KMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLV 540
           KM EKGVDCCAGY LDLVVNAYCQKKRETD SRL CDLVDEKQLKPW STYK LINKLLV
Sbjct: 481 KMVEKGVDCCAGYTLDLVVNAYCQKKRETDASRLFCDLVDEKQLKPWDSTYKALINKLLV 540

Query: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600
           RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL
Sbjct: 541 RGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVFLHL 600

Query: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAASPPPNLAS 651
           FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNS + VEAA+PPPNLAS
Sbjct: 601 FEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSMKHVEAAAPPPNLAS 651

BLAST of CmaCh04G028320 vs. TAIR 10
Match: AT3G02490.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 625.5 bits (1612), Expect = 4.6e-179
Identity = 337/655 (51.45%), Postives = 439/655 (67.02%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC 60
           MRY W  L  R++        S+ S FQV+SN    S R FS      +H+   V  R C
Sbjct: 1   MRYQWRSLLFRSYRSSPRPFLSHHSRFQVISN----STRSFSSF----LHERFGVQQRQC 60

Query: 61  -----------TPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDL 120
                        R  SSE  I +K  A  VV D+FS+    DEI K L+S+ VVISH+L
Sbjct: 61  LFALRSPLASSVSRRFSSESAIEEKLPAETVVIDVFSRLNGKDEITKELDSNDVVISHEL 120

Query: 121 VLEVLGKLESNPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKK 180
            L VL +LES+PD A RFF W    Y +KLSSKSYN ML I GVNG V+EFW L  D+KK
Sbjct: 121 ALRVLRELESSPDVAGRFFKWGLEAYPQKLSSKSYNTMLRIFGVNGLVDEFWRLVDDMKK 180

Query: 181 KGYGISKSVQNKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGN 240
           KG+G+S +V+++V +KF+KDGLE++ ERL+++FASGS+D S +K+ + VCK+V K VWG 
Sbjct: 181 KGHGVSANVRDRVGDKFKKDGLENDLERLKELFASGSMDNSVDKVCNRVCKIVMKEVWGA 240

Query: 241 DVEQQLRDMNVSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLG 300
           DVE+QLRD+ + F SD+VKM+LE L  +P KA +FFRWI ESG FKHDE+TYN MARVLG
Sbjct: 241 DVEKQLRDLKLEFKSDVVKMVLEKLDVDPRKALLFFRWIDESGSFKHDEKTYNAMARVLG 300

Query: 301 REDSIDRFWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAG--GNKPS 360
           +E  +DRF  +++E+RS GYEME+ET+  V  RFC  +MI+EAV L+ FAMAG   N P+
Sbjct: 301 KEKFLDRFQHMIEEIRSAGYEMEMETYVRVSARFCQTKMIKEAVELFEFAMAGSISNTPT 360

Query: 361 VDCLTFLLKKITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEV 420
             C + LLKKI  +K LD+ +F+R LK +T  GN + D M+  VLKSL +V R G+ NEV
Sbjct: 361 PHCCSLLLKKIVTAKKLDMDLFTRTLKAYTGNGNVVPDVMLQHVLKSLRSVDRFGQSNEV 420

Query: 421 LNAMKEYGYVFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCV 480
           L AM E GYV SG L+  +A  LS  GK DEAN+ VN +EASG + D+K  A+L+EG+C 
Sbjct: 421 LKAMNEGGYVPSGDLQSVIASGLSRKGKKDEANELVNFMEASGNHLDDKAMASLVEGHCD 480

Query: 481 AGDLAKASDCIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWH 540
           A DL +AS+C  KM  K     AGYA + +V AYC   +  D  +L  +LV + QLKPWH
Sbjct: 481 AKDLEEASECFKKMIGKEGVSYAGYAFEKLVLAYCNSFQARDVYKLFSELVKQNQLKPWH 540

Query: 541 STYKELINKLLVR-----GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFL 600
           STYK ++  LL++     G F EAL LL MMRNH FPPF+DPF+ Y+S SGT+ +A  FL
Sbjct: 541 STYKIMVRNLLMKKVARDGGFEEALSLLPMMRNHGFPPFVDPFMDYLSNSGTSAEAFAFL 600

Query: 601 KAMTSKSFPSTTVFLHLFEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTR 638
           KA+TSK FPS ++ L +FEA  ++ RH +AQD LS  P YIR +A+VLELFN+ +
Sbjct: 601 KAVTSKKFPSNSMVLRVFEAMLKSARHSEAQDLLSMSPSYIRRNAEVLELFNTMK 647

BLAST of CmaCh04G028320 vs. TAIR 10
Match: AT5G15980.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 615.1 bits (1585), Expect = 6.2e-176
Identity = 334/667 (50.07%), Postives = 441/667 (66.12%), Query Frame = 0

Query: 1   MRY-SWSLLGLRAHFR--------CQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHD 60
           MRY  W L+ LR++ R        C  + S +S  F    +P + +L+    L       
Sbjct: 1   MRYQQWRLMLLRSYHRSHLPYLSPCSQVTSISSRSFSSFIHPGIGALQQSEQLCPLRSPM 60

Query: 61  TSIVNSRYCTPRMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVL 120
           TS  N      R  SSEP + +K SA A V DIFS+    DEIRK LESSGVVIS DL L
Sbjct: 61  TSSGNLVKSVGRSFSSEPAVEEKSSAEATVIDIFSRLSGEDEIRKELESSGVVISQDLAL 120

Query: 121 EVLGKLESNPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKG 180
           +VL KLESNPD A  FF W+     E+LSSK+YN+ML ILG NG V+EFW L   +KKKG
Sbjct: 121 KVLRKLESNPDVAKSFFQWIKEASPEELSSKNYNMMLRILGGNGLVDEFWGLVDVMKKKG 180

Query: 181 YGISKSVQNKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDV 240
           +G+S +V++KV +KF+KDGLES+  RLR +F S  +D S E +   VCK+V K  WG+DV
Sbjct: 181 HGLSANVRDKVGDKFQKDGLESDLLRLRKLFTSDCLDNSAENVCDRVCKIVMKEEWGDDV 240

Query: 241 EQQLRDMNVSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGRE 300
           E+++RD+NV F SD+VKMI+E L  EP KA +FFRWI ES +FKHDE+TYN MARVLG+E
Sbjct: 241 EKRVRDLNVEFKSDLVKMIVERLDVEPRKALLFFRWIDESDLFKHDEKTYNAMARVLGKE 300

Query: 301 DSIDRFWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAG---GNKPSV 360
             +DRF  +V EMRS GYE+E+ET+  V  RFC  ++I+EAV+L+  AMAG    N P+ 
Sbjct: 301 KFLDRFQNIVVEMRSAGYEVEIETYVRVSTRFCQTKLIKEAVDLFEIAMAGSSSSNNPTP 360

Query: 361 DCLTFLLKKITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVL 420
            C   LLKKI  +K LD+ +FSRA+K++T+ GNALTDS++ +VLKSL +V R+ + NE+L
Sbjct: 361 HCFCLLLKKIVTAKILDMDLFSRAVKVYTKNGNALTDSLLKSVLKSLRSVDRVEQSNELL 420

Query: 421 NAMKEYGYVFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVA 480
             MK  GYV SG ++  +A  LS  GK DEA++FV+ +E+SG N D+K  A+L+EGYC +
Sbjct: 421 KEMKRGGYVPSGDMQSMIASSLSRKGKKDEADEFVDFMESSGNNLDDKAMASLVEGYCDS 480

Query: 481 GDLAKASDCIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHS 540
           G+L +A  C  KM        A Y+ + +V AYC K +  D  +LL   V + QLKP HS
Sbjct: 481 GNLDEALVCFEKMVGNTGVSYADYSFEKLVLAYCNKNQVRDAYKLLSAQVTKNQLKPRHS 540

Query: 541 TYKELINKLLVR-----GEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLK 600
           TYK L+  LL +     G F EAL LL +M++H FPPFIDPF+ Y S +G + +A+GFLK
Sbjct: 541 TYKSLVTNLLTKKIARDGGFEEALSLLPIMKDHGFPPFIDPFMSYFSSTGKSTEALGFLK 600

Query: 601 AMTSKSFPSTTVFLHLFEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNSTRLVEAAS 651
           AMTS +FP  +V L +FE   ++ RH +AQD LS CP YIRN+ DVLELFN+ +  E+A 
Sbjct: 601 AMTSNNFPYISVVLRVFETMMKSARHSEAQDLLSLCPNYIRNNPDVLELFNTMKPNESAV 660

BLAST of CmaCh04G028320 vs. TAIR 10
Match: AT3G48250.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 2.1e-91
Identity = 202/639 (31.61%), Postives = 333/639 (52.11%), Query Frame = 0

Query: 1   MRYSWSLLGLRAHFRCQLLLSSNSSHFQVLSNPNLPSLRYFSLLHKYPVHDTSIVNSRYC 60
           M  S ++L    H   Q+   S  S  +V  + NL S          P+   +IV SR+ 
Sbjct: 1   MYRSMAILSSLRHAYSQISTRSYLSRSKVGFSSNLSS----------PLDSFAIVPSRFL 60

Query: 61  TP-RMLSSEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLES 120
              R  SS+P+         V+ + +SK     E+ +GL    + ++H+  + VL KLE 
Sbjct: 61  WKFRTFSSKPD----SMLQLVLENDWSK-----EVEEGLRKPDMSLTHETAIYVLRKLEK 120

Query: 121 NPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQ 180
            P++A  F  WV  D G   S+  Y++ML IL     ++ FW    ++K+ G+ + +   
Sbjct: 121 YPEKAYYFLDWVLRDSGLSPSTPLYSIMLRILVQQRSMKRFWMTLREMKQGGFYLDEDTY 180

Query: 181 NKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVEQQLRDMN 240
             +  +  K+  +++A  +   +     + +   +   V  +V K  W  +VE++L++M 
Sbjct: 181 KTIYGELSKEKSKADAVAVAHFYERMLKENAMSVVAGEVSAVVTKGDWSCEVERELQEMK 240

Query: 241 VSFSSDMVKMILENLCTEPAKAFIFFRWIGESGM---FKHDEQTYNGMARVLGREDSIDR 300
           +  S + V  +L+ L   P KA  FF W+G  G    ++H   TYN   RVL R +S+  
Sbjct: 241 LVLSDNFVIRVLKELREHPLKALAFFHWVGGGGSSSGYQHSTVTYNAALRVLARPNSVAE 300

Query: 301 FWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLK 360
           FW VVDEM++ GY+M+++T+ +V R+F   RM+ E V LY + M G  KPS+   + LL+
Sbjct: 301 FWSVVDEMKTAGYDMDLDTYIKVSRQFQKSRMMAETVKLYEYMMDGPFKPSIQDCSLLLR 360

Query: 361 KITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMKEYGY 420
            ++ S + DL +  R  + +  TG +L+ ++   + +SL++VGR  E  E+  AM+  GY
Sbjct: 361 YLSGSPNPDLDLVFRVSRKYESTGKSLSKAVYDGIHRSLTSVGRFDEAEEITKAMRNAGY 420

Query: 421 VFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASD 480
                   ++ + L    + +EA   ++ +EA GC  D KTW  LI+G+C   +L KA  
Sbjct: 421 EPDNITYSQLVFGLCKAKRLEEARGVLDQMEAQGCFPDIKTWTILIQGHCKNNELDKALA 480

Query: 481 CIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINK 540
           C   M EKG D  +   LD++++ +    +    S  L ++V    +KPW STYK LI+K
Sbjct: 481 CFANMLEKGFDIDSN-LLDVLIDGFVIHNKFEGASIFLMEMVKNANVKPWQSTYKLLIDK 540

Query: 541 LLVRGEFREALKLLGMMRNHEFPPFIDPFILYVSKSGTADDAIGFLKAMTSKSFPSTTVF 600
           LL   +  EAL LL MM+   +P + + F  Y++K GT +DA  FL  ++SK  PS   +
Sbjct: 541 LLKIKKSEEALDLLQMMKKQNYPAYAEAFDGYLAKFGTLEDAKKFLDVLSSKDSPSFAAY 600

Query: 601 LHLFEAFFQAGRHGDAQDFLSKCPGYIRNHADVLELFNS 636
            H+ EAF++ GR  DA++ L  CP + + H  + ELF +
Sbjct: 601 FHVIEAFYREGRLTDAKNLLFICPHHFKTHPKISELFGA 619

BLAST of CmaCh04G028320 vs. TAIR 10
Match: AT5G64320.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 3.1e-18
Identity = 117/532 (21.99%), Postives = 214/532 (40.23%), Query Frame = 0

Query: 67  SEPEIGQKDSAHAVVFDIFSKCRDVDEIRKGLESSGVVISHDLVLEVLGKLESNPDEAIR 126
           S PEIG  DSA+   ++   K  D+D +R           + L+     +L  N   ++ 
Sbjct: 44  SSPEIGGTDSANE--WEKLLKPFDLDSLRNSFHKITPFQLYKLL-----ELPLNVSTSME 103

Query: 127 FFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYGISKSVQNKVLEKF 186
            F W     G + S   Y +++G LG NG  +    L   +K +G    +S+   ++  +
Sbjct: 104 LFSWTGSQNGYRHSFDVYQVLIGKLGANGEFKTIDRLLIQMKDEGIVFKESLFISIMRDY 163

Query: 187 EKDGLESEAERL----RDVFASGSIDKS----PEKMGSVVCKLVRKNVWGNDVEQQLRDM 246
           +K G   +  RL    R+V++     KS     E + S  C  V  NV+ + + +++   
Sbjct: 164 DKAGFPGQTTRLMLEMRNVYSCEPTFKSYNVVLEILVSGNCHKVAANVFYDMLSRKIPPT 223

Query: 247 NVSFSSDMVKMILENLC--TEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGREDSIDR 306
             +F      ++++  C   E   A    R + + G    +   Y  +   L + + ++ 
Sbjct: 224 LFTFG-----VVMKAFCAVNEIDSALSLLRDMTKHGCVP-NSVIYQTLIHSLSKCNRVNE 283

Query: 307 FWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLTFLLK 366
             ++++EM   G   + ETF++V+   C    I EA  +    +  G  P      +L+ 
Sbjct: 284 ALQLLEEMFLMGCVPDAETFNDVILGLCKFDRINEAAKMVNRMLIRGFAPDDITYGYLMN 343

Query: 367 ------KITVSKHL-------DLSMFSRALKIFTETGNALTDSMVFA-VLKSLSTVGRIG 426
                 ++  +K L       ++ +F+  +  F   G       V + ++ S   V  + 
Sbjct: 344 GLCKIGRVDAAKDLFYRIPKPEIVIFNTLIHGFVTHGRLDDAKAVLSDMVTSYGIVPDVC 403

Query: 427 EYNEVLNAMKEYGYVFSG--GLKRKVAYQLSS-------------------TGKSDEAND 486
            YN ++     YGY   G  GL  +V + + +                    GK DEA +
Sbjct: 404 TYNSLI-----YGYWKEGLVGLALEVLHDMRNKGCKPNVYSYTILVDGFCKLGKIDEAYN 463

Query: 487 FVNSLEASGCNSDNKTWAALIEGYCVAGDLAKASDCIHKMFEKGVDCCAGYALDLVVNAY 546
            +N + A G   +   +  LI  +C    + +A +   +M  KG      Y  + +++  
Sbjct: 464 VLNEMSADGLKPNTVGFNCLISAFCKEHRIPEAVEIFREMPRKGCKPDV-YTFNSLISGL 523

Query: 547 CQKKRETDGSRLLCDLVDEKQLKPWHSTYKELINKLLVRGEFREALKLLGMM 554
           C+         LL D++ E  +     TY  LIN  L RGE +EA KL+  M
Sbjct: 524 CEVDEIKHALWLLRDMISEGVVAN-TVTYNTLINAFLRRGEIKEARKLVNEM 555

BLAST of CmaCh04G028320 vs. TAIR 10
Match: AT5G65560.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 4.2e-15
Identity = 112/504 (22.22%), Postives = 206/504 (40.87%), Query Frame = 0

Query: 114 LGKLESNPDEAIRFFGWVSGDYGEKLSSKSYNLMLGILGVNGRVEEFWDLNCDIKKKGYG 173
           L  L+ +P  A+ F  W+S +   K S  SY  +L +L  NG V   + +   + K    
Sbjct: 96  LFSLDLDPKTALNFSHWISQNPRYKHSVYSYASLLTLLINNGYVGVVFKIRLLMIKSCDS 155

Query: 174 ISKSVQ-NKVLEKFEKDGLESEAERLRDVFASGSIDKSPEKMGSVVCKLVRKNVWGNDVE 233
           +  ++    +  K  KD    E   L+     G  +     +         K V+   +E
Sbjct: 156 VGDALYVLDLCRKMNKD----ERFELKYKLIIGCYNTLLNSLARFGLVDEMKQVYMEMLE 215

Query: 234 QQLRDMNVSFSSDMVKMILENLCTEPAKAFIFFRWIGESGMFKHDEQTYNGMARVLGRED 293
            ++   N+   + MV    +    E A  ++    I E+G+   D  TY  +     +  
Sbjct: 216 DKVCP-NIYTYNKMVNGYCKLGNVEEANQYV--SKIVEAGL-DPDFFTYTSLIMGYCQRK 275

Query: 294 SIDRFWKVVDEMRSHGYEMEVETFSEVLRRFCARRMIEEAVNLYVFAMAGGNKPSVDCLT 353
            +D  +KV +EM   G       ++ ++   C  R I+EA++L+V        P+V   T
Sbjct: 276 DLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYT 335

Query: 354 FLLKKITVSKHLDLSMFSRALKIFTETGNALTDSMVFAVLKSLSTVGRIGEYNEVLNAMK 413
            L+K +  S+    ++    +K   ETG          ++ SL +  +  +  E+L  M 
Sbjct: 336 VLIKSLCGSERKSEAL--NLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQML 395

Query: 414 EYGYVFSGGLKRKVAYQLSSTGKSDEANDFVNSLEASGCNSDNKTWAALIEGYCVAGDLA 473
           E G + +      +       G  ++A D V  +E+   + + +T+  LI+GYC   ++ 
Sbjct: 396 EKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYC-KSNVH 455

Query: 474 KASDCIHKMFEKGVDCCAGYALDLVVNAYCQKKRETDGSRLLCDLVDEKQLKPWHSTYKE 533
           KA   ++KM E+ V        + +++  C+        RLL  L++++ L P   TY  
Sbjct: 456 KAMGVLNKMLERKV-LPDVVTYNSLIDGQCRSGNFDSAYRLL-SLMNDRGLVPDQWTYTS 515

Query: 534 LINKLLVRGEFREALKLLGMMRNHEFPPFIDPFILYVS---KSGTADDAIGFLKAMTSKS 593
           +I+ L       EA  L   +      P +  +   +    K+G  D+A   L+ M SK+
Sbjct: 516 MIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKN 575

Query: 594 -FPSTTVFLHLFEAFFQAGRHGDA 613
             P++  F  L       G+  +A
Sbjct: 576 CLPNSLTFNALIHGLCADGKLKEA 586

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8916.5e-17851.45Pentatricopeptide repeat-containing protein At3g02490, mitochondrial OS=Arabidop... [more]
Q8LPF18.8e-17550.07Pentatricopeptide repeat-containing protein At5g15980, mitochondrial OS=Arabidop... [more]
Q9STK53.0e-9031.61Pentatricopeptide repeat-containing protein At3g48250, chloroplastic OS=Arabidop... [more]
Q9FMF64.4e-1721.99Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidop... [more]
Q9LSL96.0e-1422.22Pentatricopeptide repeat-containing protein At5g65560 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A6J1JSU20.0e+00100.00pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isofor... [more]
A0A6J1E5F80.0e+0095.85pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isofor... [more]
A0A6J1JQW60.0e+00100.00pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isofor... [more]
A0A6J1E0A60.0e+0096.42pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isofor... [more]
A0A6J1FJW50.0e+0083.08pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like OS=Cuc... [more]
Match NameE-valueIdentityDescription
XP_022990729.10.0e+00100.00pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isofor... [more]
XP_022921370.10.0e+0095.85pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like isofor... [more]
KAG6602554.10.0e+0095.70Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_023511958.10.0e+0095.08pentatricopeptide repeat-containing protein At3g02490, mitochondrial-like [Cucur... [more]
KAG7033234.10.0e+0095.55Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
Match NameE-valueIdentityDescription
AT3G02490.14.6e-17951.45Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G15980.16.2e-17650.07Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G48250.12.1e-9131.61Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G64320.13.1e-1821.99Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G65560.14.2e-1522.22Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 457..486
e-value: 6.7E-5
score: 20.8
coord: 280..310
e-value: 2.4E-4
score: 19.1
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 457..486
e-value: 2.5E-4
score: 21.1
coord: 529..556
e-value: 0.25
score: 11.7
coord: 280..308
e-value: 0.41
score: 11.0
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 277..311
score: 9.613118
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 454..488
score: 10.194067
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 232..366
e-value: 1.8E-14
score: 55.4
coord: 493..569
e-value: 7.8E-6
score: 27.3
coord: 367..492
e-value: 1.0E-12
score: 49.7
IPR044578Pentatricopeptide repeat-containing protein BIR6-likePANTHERPTHR47003OS01G0970900 PROTEINcoord: 26..643
NoneNo IPR availablePANTHERPTHR47003:SF3BNAC09G41880D PROTEINcoord: 26..643

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G028320.1CmaCh04G028320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008380 RNA splicing
molecular_function GO:0005515 protein binding