CmaCh04G027650 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G027650
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDefective in cullin neddylation protein
LocationCma_Chr04: 18601742 .. 18606818 (-)
RNA-Seq ExpressionCmaCh04G027650
SyntenyCmaCh04G027650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTTCAGTTGGACCCATTGCGTAGCATTCACAAAGAATATAAGATGAAATTGCGCAATAAACGTAATGTGTGACTGAAAAGGCAGAGGCAAAGAAGAAGGGGGGTAGAAAGAGAATCGAGAGAGAGAGGTTCCTTCTCTGTTTTCGCGTTGAGGTCTGGATTCTTCTTCCGCTTCTTGCCTCCTCGACAATCAAGACTTCCATTTCTTCTCCCTCCGTTTCTTGCAGATGAATCGCTTCGATATCTTCCAGATTTATCATCAATACTGTGGTAACAACTTCCTCCTCTTCCCCTACCGCTTCAAATTTCAGATTCTGCCTTTTTCTCTTGATTCTTGTCTGATGTCTCTTTTATTCATCATTTGCTACTGCCAATTCTCACCGAACATTGCTTTTGTTAAACCTTTCAAATCTTTCAATGTTTTCTGCCCTTTCACTATCTGTTGCAATACTTTTCTCTTCTTAGGATTTTTGGTGTTTTTCATGCAAATCTATGTCTTTGTCTTCTCGCGGATGCATGTTAACTTGGAAGATGTAATTTTGTTATAGATTTTGATGTTATATATATATATATATATATATATATATTTATCTTTCTAAAGATGGTTAGTTTCTAAGTGATTTTTTATGTTTAAGAAGTTATGATGTATTTGATTCGGAGAAGTCTGGGTGATTTTATTTTTGGGAACTGCATTAATAGTTTGGATGATAAAGAACGTTAGAAATTCAGTTTATGGTACTTATTTCGGAAAATAAGATGCATCAGGCTCAATGTCAATGTTATTTTCTGGTCAGTCAACTTAGAGAATTTGAATGAATTTTATTCTTCCAAATGAGAAAGCCCTGTCATTATTTAGGAGGGATAATCCAAGAATTAGTAATTATTGTATTATGATTTCTGATTCCACTGCAGCCCTTTAGGGGGAGAAGCTATAGACTTACCTAATTATTTCAGATCGATAAAATTTACATTATTTTTACTTACTTGTTTATAGGTATTCGATCTCAAAATGGTCTTTGTCATGGGAATGGAGGTTTTGGACATGTTGAACCACAAATGGCCAAAAGTCCAAAGCAAGCACTTGCAGAGCTCTTAATATATGTGCAATCGTCACTGCATACGGGGTATATCCACTTTGTTTTATACAACTCAATATTTCTTGTCCTTCCAAACTAAGACATTTTCTAAACATTTTTAACCAACAGACATATGAAACTAAGATAGTTTATCAACTCAATTATTAAACTAAGATGACTTTTAATATTGTAGACATGGATGTTTACTAGTTTGATTTCATTAGTTATGGTATGGTCCTGTGTTTTCTAGCAGTTCAATAAATGGAATATTACATGGAAAAAAGTTGACTTAGCTGCCTAAATACGACTTGATAAATGAGGCCATCATACGTTGTTTTCATTGCAGTATAATTTTTTTTTCTTATATTAATCTAGGTTATCTAAAAAGTATAAATTACATTTGCAGTAACTCAATCCTTCATGAACTATCAAAGCTGATGTCATATCTAAACTTGATGGTAAACCCTTCCTTCCTATCATTTTTCCTGGTTTCCTACTGTAATCTAGTTTCTTTTAATTTGGCAAGCTTTTGGGTATTGGCTGGTGGCAATATTATATTTTCAGTAAGATTCATAGTGTTTTATTCATGTGCTTCAGTTAACTTTAAGAATTTTGATTGAAAATTTTTCTGGTTTGTACTTTGAATTTGACAATTCTATTTTGTAGGTAGACTTCTCTGAATTTTCACGCTTCTATGAGTTTGTTTTCTTTGTGTGCCGAGAAAATGGTCAAAAGAATATCAGTAAGGTTCCTTCTTTTGGCTTGGATATAAAACTATGTTTTAGTTTCCTAATATGAATGATATCATGACATGTGCACTGGGTAAGTTAAAAAATTTCTTACTTTCTGATAGTTTAGTTTTTCTTCTTTTATTTGAATTACAGCCGTTGGTAGGGCAATTAAAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGGTACCATTTTCCCCCTTTTTTCTTCTCCTAATAAGGTCCCGTTTCCTTTCTTTCTTTTCACTTTGGTTGTAATGTAGTCTACAAGCTTTAAATAAAATGTATAATGATCATAATCAGGAAACCGGAATTTCCACCTGAAGTTGTTTTCATCAACGTTGGTTTATGAAATATACCTACATGCTAAATACCCATGAACGGACATTTCTATCAATGTTATTATTTGCCATTTGTAGACACGTTTGGATGCACATTCATATGTATTTTACAAAATATATGCATGTATCTCAATTTTTGAAGTTAATGAATTTGGGAAATTTTGGGGTTTTTTTTTATACCTAAGATTCTAATCTATCAAATTTCTTCTGTCTAGGTTTATGCATCCAAGTTATTTTTTTGGGTTTTATTCTTTACCTTGTTCCTTGAAATAGACGTGTTGTGGCATATGATCCAACTTATCTCAAGGATTCTAACCTAGTTAAGTTCCACAGAACAATCAGCGACACAATATTTCTGAGGATACATGGCTTCAAGTTTTGGCTTTCAGCCGTTGTGTACATGAAAATCTGGAAGGGTATGATCCTGAAGGTATTTGTATTTACATTTTTTTGTTATCTTTTAAAATATTTATTTGTGTATTAAATACGCTGAATTAAAAATGTTGAAGACTTGGGAATTACTTCAAGTAATCCGTCTCCTTATCTGTTGGAAGTTGGTCCATTGATACAATTGAATCCTATCACAGGGAAAAAGTCGCGGATAAATATATTTTGTCAATTTATGTAAGAAAATATAATACTTTTCATGGTTAATCTTCTGTTTTGGTTCTTCTACGGATGCTTGAAATATTATGACATTGCAAATATTAGTTGATCGTTTTGTTATGCTGGCATAGTATTGTTATTTTGTTTCCTCAAGTGTATGCTTAATGCTTGAAAATGTTTTTGAGGCTTGTGCATTTTTGTTTTTTAAGAAGCACATGCCTCTTCTGCGAGAGTCATACACTGCGATTTGAAGTTCAATAGTTATATGATGGCTGGAAGAATGCATCTAACAGTTGTCTGCATATTCTTCAAATATAAAAGTCCACCTTAGAATGCAATACTATAACAGCTCTGGTTGTGATAATGTAGCCCTTTATTACTTTTATCGCTAAAGCTTAAGAAAGATGCAGAAGACAAAATTTAAAGAATAGATTGATAATACAGAAAACAATTTAAGGAATAGGTTGATCCCGTTAGTGATAGTTATTACTGACGTTAAAAATGAGCTGCATAGTTTTGACTTGAGAAATTATGGATGGATACCTCCCCCCATCCTCCCGGGACTTTTATATAATACGTAGGTCTATTGTTGTTGAATTTAATTTAAATTTGTCTAATTAAATTGAGGATTATGCCTCATTTCTCTTGTAGAGGAAAGAGCCTCAAGACTACCCGTAGTCTTTTGGTGGAATGTTGGGTATGCCTGTACATAAGCATAATGTTTTTCTATTTTTCTCTACTGTACTATTTGGAGGTACTCAGTTGCATTCTTCTCTCAGGTGCTTGGCCCGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGTTTGTTTGTGGGGGATTTTACAGATGCAATGAGTTTGAAGTTGAAAGTAGTTAAATAATTCTGGCTTGGATATAGTCTCGAGTCTGACGTGGTATGGTTTAGTCTTGTACTAACACTCGATAAGCTGATAACATCTAATTATTATGCAGGCTGTCAGGATCTAATAAAATCTCAAAGTTACGTTGTAATTGTGGAGGCTCAGAATCACAGTCAGGTGCATCTGAAGATCCGTTTCCTGGTATAATAGATTACGTTCAACTGTCAAAAGAAAAAAGTACTGTTCTACAAATGGACTTCCATGCCGTGCAGAATGTTGTATGTAATAAGCTCTGTTTTACGGGTTATGATTGTGTCAGGATTAAAGATTCTCCCGGGTTTGAAGAGGAAATTACCTGAGGACTTGCAAATGGATGGGATGGTGTCTCCGAGTGATCCTACAACTGGCTCCATGGATTCGAGTCCTGTTACGAGCATCAAGAAAAGTAGATTTATGGCTTGCAAGCCAGTGAACTTGGAGATAAACGGGCCTTCATGTAATGCAGCAGCAGGTGAGAACTCAGAAATGGTTCGAAATAATGGTGCAATGGGTTGCTCCAAAGCTCCTTGTGCTGTTGAAGGTTGTCTGTCACAAGGTTTTGCTGGGCTTTTCTCGACACGTTCGTTCTTAGGATTGGATCAAGAAAGGAAATCGTCATTCACATGAGTACGAGCAATTTAGCTACCATGATTTTTACAGGTCTCATGCTCTCTTCTTTGTGATGGTACAGCATTTTTAATCGGATGAATTGATGATTTGGCAGCAAAATAGAAGAAAGGATTTTTTGAGTGTTTCTAAAGAGAACAGAAATTAGTGATTTGGCATTACGCTGTAATTGGGTCTTGGTTGGTGCATTTCAAAAAATGAATAAAAAGTGTTCAATAGTTTGTGTTTTGTTTTATAATACTCTTCTCAATCCACTTGGCTTACATGAGAGTCAAAAGGGTTTTAAAACTTCAGGTCCAAACCAGAATAGAGGCCGCCATGGTTAGGGGATTGAAGATGTGGAAAAGAGAGAAGGAACAAGGAAAGGGCCCACCGGCAACTCGAAACCTCGGAAGCTGGAGATCTGCAATGGACTGAGCTGCATTATTATTAGGTCAATCACTGACACTTTCTTTGTTTCTTTGTTTCTGAAGAAGAAGAAGAAGAAGAAGGGTGAATGAATTGAATGCGGAGGTTTGTCGTTCATCTTCCATGGCCCCCTCAATTTCCTCTCGCCTTTCACTATTATTAACTCTCATTATTCACCGCTCCTTCTCATCATCTCTATTTATTTCACCAAACTAACTCACCCTCTTCTATTTATTTCTATGTCGTTACCTTTACCTTTATCTTCCCGTAGATTTCCATAGATTTTGAGTATAGCTTTAAGTAGTCGGAATATTAATATTTCTCCTTATAT

mRNA sequence

ATGGCGGTTCAGTTGGACCCATTGCGTAGCATTCACAAAGAATATAAGATGAAATTGCGCAATAAACGTCTGGATTCTTCTTCCGCTTCTTGCCTCCTCGACAATCAAGACTTCCATTTCTTCTCCCTCCGTTTCTTGCAGATGAATCGCTTCGATATCTTCCAGATTTATCATCAATACTGTGGTATTCGATCTCAAAATGGTCTTTGTCATGGGAATGGAGGTTTTGGACATGTTGAACCACAAATGGCCAAAAGTCCAAAGCAAGCACTTGCAGAGCTCTTAATATATGTGCAATCGTCACTGCATACGGGTAACTCAATCCTTCATGAACTATCAAAGCTGATGTCATATCTAAACTTGATGGTAGACTTCTCTGAATTTTCACGCTTCTATGAGTTTGTTTTCTTTGTGTGCCGAGAAAATGGTCAAAAGAATATCACCGTTGGTAGGGCAATTAAAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGAACAATCAGCGACACAATATTTCTGAGGATACATGGCTTCAAGTTTTGGCTTTCAGCCGTTGTGTACATGAAAATCTGGAAGGGTATGATCCTGAAGGTGCTTGGCCCGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGCTGTCAGGATCTAATAAAATCTCAAAGTTACGTTGTAATTGTGGAGGCTCAGAATCACAGTCAGGTGCATCTGAAGATCCGTTTCCTGGATTAAAGATTCTCCCGGGTTTGAAGAGGAAATTACCTGAGGACTTGCAAATGGATGGGATGGTGTCTCCGAGTGATCCTACAACTGGCTCCATGGATTCGAGTCCTGTTACGAGCATCAAGAAAAGTAGATTTATGGCTTGCAAGCCAGTGAACTTGGAGATAAACGGGCCTTCATGTAATGCAGCAGCAGGTGAGAACTCAGAAATGGTTCGAAATAATGGTGCAATGGGTTGCTCCAAAGCTCCTTGTGCTGTTGAAGGTTGTCTGTCACAAGGTTTTGCTGGGCTTTTCTCGACACGTTCGTTCTTAGGATTGGATCAAGAAAGGAAATCGTCATTCACATGAGTACGAGCAATTTAGCTACCATGATTTTTACAGGTCCAAACCAGAATAGAGGCCGCCATGGTTAGGGGATTGAAGATGTGGAAAAGAGAGAAGGAACAAGGAAAGGGCCCACCGGCAACTCGAAACCTCGGAAGCTGGAGATCTGCAATGGACTGAGCTGCATTATTATTAGGTCAATCACTGACACTTTCTTTGTTTCTTTGTTTCTGAAGAAGAAGAAGAAGAAGAAGGGTGAATGAATTGAATGCGGAGGTTTGTCGTTCATCTTCCATGGCCCCCTCAATTTCCTCTCGCCTTTCACTATTATTAACTCTCATTATTCACCGCTCCTTCTCATCATCTCTATTTATTTCACCAAACTAACTCACCCTCTTCTATTTATTTCTATGTCGTTACCTTTACCTTTATCTTCCCGTAGATTTCCATAGATTTTGAGTATAGCTTTAAGTAGTCGGAATATTAATATTTCTCCTTATAT

Coding sequence (CDS)

ATGGCGGTTCAGTTGGACCCATTGCGTAGCATTCACAAAGAATATAAGATGAAATTGCGCAATAAACGTCTGGATTCTTCTTCCGCTTCTTGCCTCCTCGACAATCAAGACTTCCATTTCTTCTCCCTCCGTTTCTTGCAGATGAATCGCTTCGATATCTTCCAGATTTATCATCAATACTGTGGTATTCGATCTCAAAATGGTCTTTGTCATGGGAATGGAGGTTTTGGACATGTTGAACCACAAATGGCCAAAAGTCCAAAGCAAGCACTTGCAGAGCTCTTAATATATGTGCAATCGTCACTGCATACGGGTAACTCAATCCTTCATGAACTATCAAAGCTGATGTCATATCTAAACTTGATGGTAGACTTCTCTGAATTTTCACGCTTCTATGAGTTTGTTTTCTTTGTGTGCCGAGAAAATGGTCAAAAGAATATCACCGTTGGTAGGGCAATTAAAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGAACAATCAGCGACACAATATTTCTGAGGATACATGGCTTCAAGTTTTGGCTTTCAGCCGTTGTGTACATGAAAATCTGGAAGGGTATGATCCTGAAGGTGCTTGGCCCGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGCTGTCAGGATCTAATAAAATCTCAAAGTTACGTTGTAATTGTGGAGGCTCAGAATCACAGTCAGGTGCATCTGAAGATCCGTTTCCTGGATTAAAGATTCTCCCGGGTTTGAAGAGGAAATTACCTGAGGACTTGCAAATGGATGGGATGGTGTCTCCGAGTGATCCTACAACTGGCTCCATGGATTCGAGTCCTGTTACGAGCATCAAGAAAAGTAGATTTATGGCTTGCAAGCCAGTGAACTTGGAGATAAACGGGCCTTCATGTAATGCAGCAGCAGGTGAGAACTCAGAAATGGTTCGAAATAATGGTGCAATGGGTTGCTCCAAAGCTCCTTGTGCTGTTGAAGGTTGTCTGTCACAAGGTTTTGCTGGGCTTTTCTCGACACGTTCGTTCTTAGGATTGGATCAAGAAAGGAAATCGTCATTCACATGA

Protein sequence

MAVQLDPLRSIHKEYKMKLRNKRLDSSSASCLLDNQDFHFFSLRFLQMNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNSILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGSNKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT
Homology
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BLAST of CmaCh04G027650 vs. ExPASy Swiss-Prot
Match: Q9MBG8 (Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=AAR3 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.6e-77
Identity = 158/312 (50.64%), Postives = 194/312 (62.18%), Query Frame = 0 

Query: 50  RFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNSIL 109
           RFDIF+IY ++C IRS   LC+     G  E Q     K+A+ +LL  V++     NSI 
Sbjct: 9   RFDIFEIYRRFCAIRSGQQLCNKKPCDGE-ESQRFNLSKEAITQLLYLVENKFQARNSIF 68

Query: 110 HELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLLNQ 169
            EL KLMS L+LMVDF+EF+ FY+FVFF+CRENGQKNIT+ RAI AW+LVL GRFRLLN+
Sbjct: 69  DELFKLMSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNR 128

Query: 170 WCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGSNK 229
           WCDF+E NQRHNISEDTW QVLAFSRCVHENLEGYD EGAWPVLIDDFVEHMY + G NK
Sbjct: 129 WCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSILGPNK 188

Query: 230 ISKLRCNCGGSESQSGASED---------PFPGLKILPGLKRKLPEDLQMDGMVSPSDPT 289
            + L C CG +ES+S   ++         P  GL+ +PGLKRK  +          +D  
Sbjct: 189 DTSLFCKCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKRKTSK---------KNDEE 248

Query: 290 TGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEG 349
               D   + +   S  +  K +    N P C+                  SK+PC++E 
Sbjct: 249 EEDEDEEVLETQNSSSLLNFKRIKTS-NSPRCS------------------SKSPCSIER 291

Query: 350 CLSQGFAGLFST 353
            LSQGFA L ST
Sbjct: 309 SLSQGFASLLST 291

[Close]
BLAST of CmaCh04G027650 vs. ExPASy Swiss-Prot
Match: Q9VUQ8 (DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2)

HSP 1 Score: 104.0 bits (258), Expect = 3.7e-21
Identity = 50/124 (40.32%), Postives = 69/124 (55.65%), Query Frame = 0 

Query: 124 DFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLLNQWCDFVENNQRHNIS 183
           D  +F  FY F F   ++ GQK I +  AI  W +VL GRF+ L+ WC F+E   +  IS
Sbjct: 146 DAGKFKDFYHFTFNYAKDPGQKGIDLEMAIAYWCIVLSGRFKFLDIWCQFLEEKHKRAIS 205

Query: 184 EDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGSNKISKLRCNCGGSESQ 243
            DTW  +L F+  + + +  YD EGAWPVLIDDFVE       ++ + +      G + Q
Sbjct: 206 RDTWNLLLDFATNIDDRMSNYDSEGAWPVLIDDFVEWCQE---NDHLKEDSSPASGYQQQ 265

Query: 244 SGAS 248
           S AS
Sbjct: 266 SSAS 266

[Close]
BLAST of CmaCh04G027650 vs. ExPASy Swiss-Prot
Match: Q6PH85 (DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.2e-19
Identity = 45/125 (36.00%), Postives = 71/125 (56.80%), Query Frame = 0 

Query: 97  YVQSSLHTGNSILHELSKLMSYLNL-MVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKA 156
           ++      G   + +L  L+  L   + D ++F  FY+F F   +  GQK + +  A+  
Sbjct: 122 FLDGMTELGCDSMEKLKALLPRLEQELKDTAKFKDFYQFTFTFAKNPGQKGLDLEMAVAY 181

Query: 157 WRLVLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLID 216
           W+LVL GRF+ L+ W  F+  + + +I  DTW  +L F   + +++  YD EGAWPVLID
Sbjct: 182 WKLVLSGRFKFLDLWNTFLMEHHKRSIPRDTWNLLLDFGNMIADDMSNYDEEGAWPVLID 241

Query: 217 DFVEH 221
           DFVE+
Sbjct: 242 DFVEY 246

[Close]
BLAST of CmaCh04G027650 vs. ExPASy Swiss-Prot
Match: Q5ZKU1 (DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.5e-19
Identity = 42/92 (45.65%), Postives = 59/92 (64.13%), Query Frame = 0 

Query: 128 FSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTW 187
           F  FY+F F   +  GQK + +  AI  W LVL+GRF+ L+ W  F+  + + +I +DTW
Sbjct: 154 FKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTW 213

Query: 188 LQVLAFSRCVHENLEGYDPEGAWPVLIDDFVE 220
             +L FS  + +++  YD EGAWPVLIDDFVE
Sbjct: 214 NLLLDFSTMIADDMSNYDEEGAWPVLIDDFVE 245

[Close]
BLAST of CmaCh04G027650 vs. ExPASy Swiss-Prot
Match: Q96GG9 (DCN1-like protein 1 OS=Homo sapiens OX=9606 GN=DCUN1D1 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.5e-19
Identity = 42/92 (45.65%), Postives = 59/92 (64.13%), Query Frame = 0 

Query: 128 FSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTW 187
           F  FY+F F   +  GQK + +  AI  W LVL+GRF+ L+ W  F+  + + +I +DTW
Sbjct: 154 FKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTW 213

Query: 188 LQVLAFSRCVHENLEGYDPEGAWPVLIDDFVE 220
             +L FS  + +++  YD EGAWPVLIDDFVE
Sbjct: 214 NLLLDFSTMIADDMSNYDEEGAWPVLIDDFVE 245

[Close]
BLAST of CmaCh04G027650 vs. ExPASy TrEMBL
Match: A0A6J1JQF9 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111486895 PE=4 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.0e-186
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0 

Query: 48  MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 107
           MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS
Sbjct: 1   MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 60

Query: 108 ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 167
           ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 168 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 227
           NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 228 NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 287
           NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS
Sbjct: 181 NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 240

Query: 288 PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA 347
           PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA
Sbjct: 241 PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA 300

Query: 348 GLFSTRSFLGLDQERKSSFT 368
           GLFSTRSFLGLDQERKSSFT
Sbjct: 301 GLFSTRSFLGLDQERKSSFT 320

[Close]
BLAST of CmaCh04G027650 vs. ExPASy TrEMBL
Match: A0A6J1JRF7 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111486895 PE=4 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.6e-184
Identity = 320/332 (96.39%), Postives = 320/332 (96.39%), Query Frame = 0 

Query: 48  MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 107
           MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS
Sbjct: 1   MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 60

Query: 108 ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 167
           ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 168 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 227
           NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 228 NKISKLRCNCGGSESQSGASEDPFPG------------LKILPGLKRKLPEDLQMDGMVS 287
           NKISKLRCNCGGSESQSGASEDPFPG            LKILPGLKRKLPEDLQMDGMVS
Sbjct: 181 NKISKLRCNCGGSESQSGASEDPFPGIIDYVQLSKEKRLKILPGLKRKLPEDLQMDGMVS 240

Query: 288 PSDPTTGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAP 347
           PSDPTTGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAP
Sbjct: 241 PSDPTTGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAP 300

Query: 348 CAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT 368
           CAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT
Sbjct: 301 CAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT 332

[Close]
BLAST of CmaCh04G027650 vs. ExPASy TrEMBL
Match: A0A6J1JNF7 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111486895 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.6e-179
Identity = 312/320 (97.50%), Postives = 312/320 (97.50%), Query Frame = 0 

Query: 48  MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 107
           MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS
Sbjct: 1   MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 60

Query: 108 ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 167
           ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 168 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 227
           NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 228 NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 287
           NKISKLRCNCGGSESQS        GLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS
Sbjct: 181 NKISKLRCNCGGSESQS--------GLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 240

Query: 288 PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA 347
           PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA
Sbjct: 241 PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA 300

Query: 348 GLFSTRSFLGLDQERKSSFT 368
           GLFSTRSFLGLDQERKSSFT
Sbjct: 301 GLFSTRSFLGLDQERKSSFT 312

[Close]
BLAST of CmaCh04G027650 vs. ExPASy TrEMBL
Match: A0A6J1GSP1 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111457165 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.2e-178
Identity = 310/324 (95.68%), Postives = 314/324 (96.91%), Query Frame = 0 

Query: 48  MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 107
           MNRFDI QIYHQYCGIRSQNGLCHGNGGFGHVE QMAKS KQALAELLIYVQSSLHTGNS
Sbjct: 1   MNRFDIVQIYHQYCGIRSQNGLCHGNGGFGHVETQMAKSSKQALAELLIYVQSSLHTGNS 60

Query: 108 ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 167
           ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 168 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 227
           NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 228 NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 287
           NKISKLRC+CGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSM+ S
Sbjct: 181 NKISKLRCSCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMELS 240

Query: 288 PVTSIKKSRFMACKPVNLEINGPSCNAAAGE----NSEMVRNNGAMGCSKAPCAVEGCLS 347
           PVTSIKKSRFMACKPVNLEINGPSCNAAA      N+E+VRNNGAMGCSKAPCAVEGCLS
Sbjct: 241 PVTSIKKSRFMACKPVNLEINGPSCNAAAAAAADGNTEIVRNNGAMGCSKAPCAVEGCLS 300

Query: 348 QGFAGLFSTRSFLGLDQERKSSFT 368
           QGFAGLFSTRSFLGLDQERKSSFT
Sbjct: 301 QGFAGLFSTRSFLGLDQERKSSFT 324

[Close]
BLAST of CmaCh04G027650 vs. ExPASy TrEMBL
Match: A0A6J1GU86 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111457165 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.7e-176
Identity = 310/333 (93.09%), Postives = 314/333 (94.29%), Query Frame = 0 

Query: 48  MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 107
           MNRFDI QIYHQYCGIRSQNGLCHGNGGFGHVE QMAKS KQALAELLIYVQSSLHTGNS
Sbjct: 1   MNRFDIVQIYHQYCGIRSQNGLCHGNGGFGHVETQMAKSSKQALAELLIYVQSSLHTGNS 60

Query: 108 ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 167
           ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 168 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 227
           NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 228 NKISKLRCNCGGSESQSGASEDPFP---------GLKILPGLKRKLPEDLQMDGMVSPSD 287
           NKISKLRC+CGGSESQSGASEDPFP         GLKILPGLKRKLPEDLQMDGMVSPSD
Sbjct: 181 NKISKLRCSCGGSESQSGASEDPFPGIIDFVLLSGLKILPGLKRKLPEDLQMDGMVSPSD 240

Query: 288 PTTGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGE----NSEMVRNNGAMGCSKA 347
           PTTGSM+ SPVTSIKKSRFMACKPVNLEINGPSCNAAA      N+E+VRNNGAMGCSKA
Sbjct: 241 PTTGSMELSPVTSIKKSRFMACKPVNLEINGPSCNAAAAAAADGNTEIVRNNGAMGCSKA 300

Query: 348 PCAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT 368
           PCAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT
Sbjct: 301 PCAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT 333

[Close]
BLAST of CmaCh04G027650 vs. NCBI nr
Match: XP_022989824.1 (uncharacterized protein LOC111486895 isoform X2 [Cucurbita maxima])

HSP 1 Score: 662.5 bits (1708), Expect = 2.1e-186
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0 

Query: 48  MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 107
           MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS
Sbjct: 1   MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 60

Query: 108 ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 167
           ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 168 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 227
           NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 228 NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 287
           NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS
Sbjct: 181 NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 240

Query: 288 PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA 347
           PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA
Sbjct: 241 PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA 300

Query: 348 GLFSTRSFLGLDQERKSSFT 368
           GLFSTRSFLGLDQERKSSFT
Sbjct: 301 GLFSTRSFLGLDQERKSSFT 320

[Close]
BLAST of CmaCh04G027650 vs. NCBI nr
Match: XP_022989823.1 (uncharacterized protein LOC111486895 isoform X1 [Cucurbita maxima])

HSP 1 Score: 653.7 bits (1685), Expect = 9.6e-184
Identity = 320/332 (96.39%), Postives = 320/332 (96.39%), Query Frame = 0 

Query: 48  MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 107
           MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS
Sbjct: 1   MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 60

Query: 108 ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 167
           ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 168 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 227
           NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 228 NKISKLRCNCGGSESQSGASEDPFPG------------LKILPGLKRKLPEDLQMDGMVS 287
           NKISKLRCNCGGSESQSGASEDPFPG            LKILPGLKRKLPEDLQMDGMVS
Sbjct: 181 NKISKLRCNCGGSESQSGASEDPFPGIIDYVQLSKEKRLKILPGLKRKLPEDLQMDGMVS 240

Query: 288 PSDPTTGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAP 347
           PSDPTTGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAP
Sbjct: 241 PSDPTTGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAP 300

Query: 348 CAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT 368
           CAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT
Sbjct: 301 CAVEGCLSQGFAGLFSTRSFLGLDQERKSSFT 332

[Close]
BLAST of CmaCh04G027650 vs. NCBI nr
Match: KAG6602497.1 (DCN1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 642.1 bits (1655), Expect = 2.9e-180
Identity = 311/321 (96.88%), Postives = 315/321 (98.13%), Query Frame = 0 

Query: 47  QMNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGN 106
           +MNRFDI QIYHQYCGIRSQNGLCHGNGGFGHVE QMAKS KQALAELLIYVQSSLHTGN
Sbjct: 19  KMNRFDIVQIYHQYCGIRSQNGLCHGNGGFGHVETQMAKSSKQALAELLIYVQSSLHTGN 78

Query: 107 SILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRL 166
           SILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRL
Sbjct: 79  SILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRL 138

Query: 167 LNQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSG 226
           LNQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSG
Sbjct: 139 LNQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSG 198

Query: 227 SNKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDS 286
           SNKISKLRC+CGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDG VSPSDPTTGSM+ 
Sbjct: 199 SNKISKLRCSCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGRVSPSDPTTGSMEL 258

Query: 287 SPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGF 346
           SPVTSIKKSRFMACKPVNLEINGPSCNAAA EN+EMVRNNGAMGCSKAPCAVEGCLSQGF
Sbjct: 259 SPVTSIKKSRFMACKPVNLEINGPSCNAAADENTEMVRNNGAMGCSKAPCAVEGCLSQGF 318

Query: 347 AGLFSTRSFLGLDQERKSSFT 368
           AGLFSTRSFLGLDQERKSSFT
Sbjct: 319 AGLFSTRSFLGLDQERKSSFT 339

[Close]
BLAST of CmaCh04G027650 vs. NCBI nr
Match: XP_023533447.1 (uncharacterized protein LOC111795323 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639.0 bits (1647), Expect = 2.4e-179
Identity = 309/320 (96.56%), Postives = 314/320 (98.12%), Query Frame = 0 

Query: 48  MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 107
           MNRFDI QIYHQYCGIRSQNGLCHGNGGFGHVE QMAKS KQAL+ELLIYVQSSLHTGNS
Sbjct: 1   MNRFDIVQIYHQYCGIRSQNGLCHGNGGFGHVETQMAKSSKQALSELLIYVQSSLHTGNS 60

Query: 108 ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 167
           ILHELSKLMSYLNL VDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLTVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 168 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 227
           NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 228 NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 287
           NKISKLRC+CGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSM+ S
Sbjct: 181 NKISKLRCSCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMELS 240

Query: 288 PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA 347
           PVTSIKKSRFMACKPVNLEINGPSCNAAA EN+EMVRNNGAMGCSKAPCAVEGCLSQGFA
Sbjct: 241 PVTSIKKSRFMACKPVNLEINGPSCNAAADENTEMVRNNGAMGCSKAPCAVEGCLSQGFA 300

Query: 348 GLFSTRSFLGLDQERKSSFT 368
           GLFSTRSFLGLD+ERKSSFT
Sbjct: 301 GLFSTRSFLGLDKERKSSFT 320

[Close]
BLAST of CmaCh04G027650 vs. NCBI nr
Match: XP_022989825.1 (uncharacterized protein LOC111486895 isoform X3 [Cucurbita maxima])

HSP 1 Score: 637.9 bits (1644), Expect = 5.4e-179
Identity = 312/320 (97.50%), Postives = 312/320 (97.50%), Query Frame = 0 

Query: 48  MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 107
           MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS
Sbjct: 1   MNRFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNS 60

Query: 108 ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 167
           ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL
Sbjct: 61  ILHELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLL 120

Query: 168 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 227
           NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS
Sbjct: 121 NQWCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGS 180

Query: 228 NKISKLRCNCGGSESQSGASEDPFPGLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 287
           NKISKLRCNCGGSESQS        GLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS
Sbjct: 181 NKISKLRCNCGGSESQS--------GLKILPGLKRKLPEDLQMDGMVSPSDPTTGSMDSS 240

Query: 288 PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA 347
           PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA
Sbjct: 241 PVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEGCLSQGFA 300

Query: 348 GLFSTRSFLGLDQERKSSFT 368
           GLFSTRSFLGLDQERKSSFT
Sbjct: 301 GLFSTRSFLGLDQERKSSFT 312

[Close]
BLAST of CmaCh04G027650 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 291.2 bits (744), Expect = 1.2e-78
Identity = 158/312 (50.64%), Postives = 194/312 (62.18%), Query Frame = 0 

Query: 50  RFDIFQIYHQYCGIRSQNGLCHGNGGFGHVEPQMAKSPKQALAELLIYVQSSLHTGNSIL 109
           RFDIF+IY ++C IRS   LC+     G  E Q     K+A+ +LL  V++     NSI 
Sbjct: 9   RFDIFEIYRRFCAIRSGQQLCNKKPCDGE-ESQRFNLSKEAITQLLYLVENKFQARNSIF 68

Query: 110 HELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGRFRLLNQ 169
            EL KLMS L+LMVDF+EF+ FY+FVFF+CRENGQKNIT+ RAI AW+LVL GRFRLLN+
Sbjct: 69  DELFKLMSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNR 128

Query: 170 WCDFVENNQRHNISEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRLSGSNK 229
           WCDF+E NQRHNISEDTW QVLAFSRCVHENLEGYD EGAWPVLIDDFVEHMY + G NK
Sbjct: 129 WCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSILGPNK 188

Query: 230 ISKLRCNCGGSESQSGASED---------PFPGLKILPGLKRKLPEDLQMDGMVSPSDPT 289
            + L C CG +ES+S   ++         P  GL+ +PGLKRK  +          +D  
Sbjct: 189 DTSLFCKCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKRKTSK---------KNDEE 248

Query: 290 TGSMDSSPVTSIKKSRFMACKPVNLEINGPSCNAAAGENSEMVRNNGAMGCSKAPCAVEG 349
               D   + +   S  +  K +    N P C+                  SK+PC++E 
Sbjct: 249 EEDEDEEVLETQNSSSLLNFKRIKTS-NSPRCS------------------SKSPCSIER 291

Query: 350 CLSQGFAGLFST 353
            LSQGFA L ST
Sbjct: 309 SLSQGFASLLST 291

[Close]
BLAST of CmaCh04G027650 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 100.5 bits (249), Expect = 2.9e-21
Identity = 45/100 (45.00%), Postives = 62/100 (62.00%), Query Frame = 0 

Query: 124 DFSEFSRFYEFVFFVCRENGQKNITVGRAIKAWRLVLDGR-FRLLNQWCDFVENNQRHNI 183
           D  +F   Y F F   +E GQK++ +  AI  W+L+   R + L+  WCDF+++     I
Sbjct: 144 DEQKFHEIYNFAFGWAKEKGQKSLALDTAIGMWQLLFAEREWPLVTHWCDFLQDRHNKAI 203

Query: 184 SEDTWLQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMY 223
           S+DTW Q+L FSR V   L  YD EGAWP LID+FVE++Y
Sbjct: 204 SKDTWAQLLEFSRMVDPVLSNYDAEGAWPYLIDEFVEYLY 243

[Close]
BLAST of CmaCh04G027650 vs. TAIR 10
Match: AT1G15860.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 50.8 bits (120), Expect = 2.7e-06
Identity = 28/98 (28.57%), Postives = 53/98 (54.08%), Query Frame = 0 

Query: 126 SEFSRFYEFVFFVC-RENGQKNITVGRAIKAWRLVLDGRFRL-LNQWCDFVE-NNQRHNI 185
           S F+ FY + F  C  E  QK+I +    +   +V+   FR  ++ + ++++  N    I
Sbjct: 123 SNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQNDYKVI 182

Query: 186 SEDTWLQVLAF-SRCVHENLEGYDPEGAWPVLIDDFVE 220
           + D W+ +  F +     ++  Y+PE AWP+++D+FVE
Sbjct: 183 NMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVE 220

[Close]
BLAST of CmaCh04G027650 vs. TAIR 10
Match: AT1G15860.3 (Domain of unknown function (DUF298) )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 30/107 (28.04%), Postives = 49/107 (45.79%), Query Frame = 0 

Query: 126 SEFSRFYEFVFFVC-RENGQKNITVGRAIKAWRLVLDGRFR--------LLNQWC---DF 185
           S F+ FY + F  C  E  QK+I +    +   +V+   FR         L  W      
Sbjct: 123 SNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKVWITQKSH 182

Query: 186 VENNQRHNISEDTWLQVLAF-SRCVHENLEGYDPEGAWPVLIDDFVE 220
           +  N    I+ D W+ +  F +     ++  Y+PE AWP+++D+FVE
Sbjct: 183 IIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVE 229

The following BLAST results are available for this feature:
BLAST of CmaCh04G027650 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9MBG81.6e-7750.64Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9VUQ83.7e-2140.32DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2[more]
Q6PH851.2e-1936.00DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1[more]
Q5ZKU13.5e-1945.65DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1[more]
Q96GG93.5e-1945.65DCN1-like protein 1 OS=Homo sapiens OX=9606 GN=DCUN1D1 PE=1 SV=1[more]
BLAST of CmaCh04G027650 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JQF91.0e-186100.00Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111486... [more]
A0A6J1JRF74.6e-18496.39Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111486... [more]
A0A6J1JNF72.6e-17997.50Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111486... [more]
A0A6J1GSP12.2e-17895.68Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1GU864.7e-17693.09Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
BLAST of CmaCh04G027650 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022989824.12.1e-186100.00uncharacterized protein LOC111486895 isoform X2 [Cucurbita maxima][more]
XP_022989823.19.6e-18496.39uncharacterized protein LOC111486895 isoform X1 [Cucurbita maxima][more]
KAG6602497.12.9e-18096.88DCN1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023533447.12.4e-17996.56uncharacterized protein LOC111795323 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022989825.15.4e-17997.50uncharacterized protein LOC111486895 isoform X3 [Cucurbita maxima][more]
BLAST of CmaCh04G027650 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G28970.11.2e-7850.64Domain of unknown function (DUF298) [more]
AT3G12760.12.9e-2145.00CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP... [more]
AT1G15860.12.7e-0628.57Domain of unknown function (DUF298) [more]
AT1G15860.31.3e-0528.04Domain of unknown function (DUF298) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005176Potentiating neddylation domainPFAMPF03556Cullin_bindingcoord: 123..220
e-value: 7.2E-29
score: 100.7
IPR005176Potentiating neddylation domainPROSITEPS51229DCUN1coord: 3..222
score: 20.578547
IPR042460DCN1-like, PONY binding domainGENE3D1.10.238.200Cullin, PONY binding domaincoord: 124..231
e-value: 1.3E-34
score: 120.6
IPR014764Defective-in-cullin neddylation proteinPANTHERPTHR12281RP42 RELATEDcoord: 49..337
NoneNo IPR availablePANTHERPTHR12281:SF31DEFECTIVE IN CULLIN NEDDYLATION PROTEINcoord: 49..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G027650.1CmaCh04G027650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051443 positive regulation of ubiquitin-protein transferase activity
biological_process GO:0045116 protein neddylation
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0097602 cullin family protein binding
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0032182 ubiquitin-like protein binding