CmaCh04G027340 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G027340
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Metalloendoproteinase 2-MMP-like
Location	Cma_Chr04: 18397862 .. 18399888 (-)
RNA-Seq Expression	CmaCh04G027340
Synteny	CmaCh04G027340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATTGATGTAGTTTGAATATCTCTAGTTTTTAGTCATCTTTGGTTGAGAGATGCTAATAATGGAGGCGGAGGGGGGGCTTCCATAGGTAAATCATGTGAAGTTTTTCCTATATTCTTCTCTCCATTCCATTCCATTTCATTCGTGTCACTTAAGCCTCTTCTTAGTGTTCGAGTCTTGAGAGATTAAAGAAAGTAGGTATGTATAACAAGACTCATTTAGTATATATATATATTTATGAAAACTTGGTCTCCTTTCCCATTCACTACATTTGTTTGAACCAAACCTTGGAGGGGAGCGGAGATGGAGGATATAATATCATCCGTATCAAGAGTAACTTCTCCTAATTCTCTCTATATTAATTGGCCATTGCTTCTAACCCCTGTTAATCCTGAATGGGCTAATTAAGAGAGTAATTATGGGTTATAAGTGAAAAATATAGTATCTAATAGTATGAGACATTTTGGGAAAACTAAAAGAAAAACCATGAGAGTTTGTGCTTAGAACAAATAATATCATGTCATTGTGGAGGGGTATGGTTCTCAACATGGTATTAAAGTCATGTCAATAGAATACTCAAGTGTGCGAACAAACGGTGTACTTTATTTAAGTGCTCCATTTTATTTTAGGGCTCAAGAAAAAGGAGCCAAGCCTTGATTAAGGGGATACTGTTCGAGGGCTATTTAGGCCTAAGGGGAGGGGAGGCTCCATATGAGGAAAAGATTGTTGAGAATTATTGAGAGGGAAGTTGACTAATTAGTGGCTATTGTTTTGTTTTATTGCAAAATTTTGTTATAATTTAGTAAAATTCATGTTAATGATAGATTGTCCCCGGGTGGGGTGAGATGAGGAAGCCTACACCAACTTCGTCCCAGTCTTTAATTCATTCTTTATTCCTATGAAATTCTCCATTTATTTTCACAAACAAAAGTCTTCCATACAAAAAAGTGTTGGCATAACTTAGATAATTACAACCATAGTCTCAAAATCTTAAACGATGCAATATAGAAGAAATAATATCTATAAAAATCTCCAAACTTCATAGACTCTTCAACCATTCGTTGTATGCAACGTATTTGAGACTTTTGGTGCAATTTTTGGTACCTCTGGCCTGTTATTTCTAAACAGATATCAAAATAAGCTTGGAAAGTGGTGAACATGGAGACGCCCATCCTTTCGACAGTGTTGGAGGAGTCATAGCTCGTGCCTTTTCGCCCACAGATGGACGTTACATTTTGATGGCGTTGAGTCATGGACTATTGCTGCCGTTCCTAATTCGTTTGATATGGAGACCGTGGCGTTGCATGAGATTGGACACCTTATGGGCCTTCACCACAGCTCAGTGGAAGGGGGTATTATGTTGCCGACCATCATGGGAGGAACTACCAAGGGTTTGCATGCCCACGACGTTGCAGCCATTAAGGCTTTATACAATGCTCTACTTCTTGACTTCTCTTTAATGTATTAGAATTGACTTCAGACTCACGTCTACCTCTAGCATATATTGTTTTCTTTTTCGAACTTCCCTCAAGATTTCAAAAATACGTTTGTAAGAGATAAATTTTCACTCTTATAAACAATGTTTCATTCTTCTACCAAGCCGATGAGAGATCTCACAATCTATCTCTTTTGAAATCCAATATTCTCGTTGACAATTGTTCATCTCATCAATCGATGTGGTATCTCACAATCTGCCTTCATTTCTTTTTCTTTCATTTATTGATATTTATTTGTTCTTATTTCAATCAATATAAATTTTGTGAAATTATAAATAATTAAATTTGGAATCAAATATAAATTTACACTCATCCCAAATTTAATTTGAAGTTGATAAATCCTGGATTAGAATGTCAGATTTCTTTATTATGAAAATTCCCAAATAATTATACTTAATTTTGTTAAATTGAGATATGAAATTTCGGACAAAAATAAAATTTAAATCCAAATGTTAGGCCCAAAGCCATTAAAGCTCAAGTTCAATGGTTGAGCCCAAAAGCCGTTACAGCCCAATTCATGGGTGCATGA

mRNA sequence

ATGGGATTGATTCATCTTTGGTTGAGAGATGCTAATAATGGAGGCGGAGGGGGGGCTTCCATAGATATCAAAATAAGCTTGGAAAGTGGTGAACATGGAGACGCCCATCCTTTCGACAGTGTTGGAGGAGTCATAGCTCGTGCCTTTTCGCCCACAGATGGACGTTACATTTTGATGGCGTTGAGTCATGGACTATTGCTGCCGTTCCTAATTCGTTTGATATGGAGACCGTGGCGTTGCATGAGATTGGACACCTTATGGGCCTTCACCACAGCTCAGTGGAAGGGGGTATTATGTTGCCGACCATCATGGGAGGAACTACCAAGGGTTTGCATGCCCACGACGCCCAAAGCCATTAAAGCTCAAGTTCAATGGTTGAGCCCAAAAGCCGTTACAGCCCAATTCATGGGTGCATGA

Coding sequence (CDS)

ATGGGATTGATTCATCTTTGGTTGAGAGATGCTAATAATGGAGGCGGAGGGGGGGCTTCCATAGATATCAAAATAAGCTTGGAAAGTGGTGAACATGGAGACGCCCATCCTTTCGACAGTGTTGGAGGAGTCATAGCTCGTGCCTTTTCGCCCACAGATGGACGTTACATTTTGATGGCGTTGAGTCATGGACTATTGCTGCCGTTCCTAATTCGTTTGATATGGAGACCGTGGCGTTGCATGAGATTGGACACCTTATGGGCCTTCACCACAGCTCAGTGGAAGGGGGTATTATGTTGCCGACCATCATGGGAGGAACTACCAAGGGTTTGCATGCCCACGACGCCCAAAGCCATTAAAGCTCAAGTTCAATGGTTGAGCCCAAAAGCCGTTACAGCCCAATTCATGGGTGCATGA

Protein sequence

MGLIHLWLRDANNGGGGGASIDIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMALSHGLLLPFLIRLIWRPWRCMRLDTLWAFTTAQWKGVLCCRPSWEELPRVCMPTTPKAIKAQVQWLSPKAVTAQFMGA

Homology

[Close]

BLAST of CmaCh04G027340 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 2.0e-04
Identity = 20/34 (58.82%), Postives = 24/34 (70.59%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGR 56
           DIKI   +G+HGD  PFD V GV+A  FSP +GR
Sbjct: 189 DIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGR 222

[Close]

BLAST of CmaCh04G027340 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 2.0e-04
Identity = 19/34 (55.88%), Postives = 24/34 (70.59%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGR 56
           +IKI   S  HGD +PFD  GG++  AF+PTDGR
Sbjct: 193 NIKILFASKNHGDPYPFDGPGGILGHAFAPTDGR 226

[Close]

BLAST of CmaCh04G027340 vs. ExPASy Swiss-Prot
Match: O18767 (Matrix metalloproteinase-20 OS=Bos taurus OX=9913 GN=MMP20 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 5.9e-04
Identity = 18/33 (54.55%), Postives = 25/33 (75.76%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDG 55
           DI IS E+G+HGD++PFD   G +A AF+P +G
Sbjct: 164 DIMISFETGDHGDSYPFDGPRGTLAHAFAPGEG 196

[Close]

BLAST of CmaCh04G027340 vs. ExPASy Swiss-Prot
Match: P57748 (Matrix metalloproteinase-20 OS=Mus musculus OX=10090 GN=Mmp20 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 5.9e-04
Identity = 18/33 (54.55%), Postives = 25/33 (75.76%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDG 55
           DI IS E+G+HGD++PFD   G +A AF+P +G
Sbjct: 165 DIMISFETGDHGDSYPFDGPRGTLAHAFAPGEG 197

[Close]

BLAST of CmaCh04G027340 vs. ExPASy Swiss-Prot
Match: P79287 (Matrix metalloproteinase-20 OS=Sus scrofa OX=9823 GN=MMP20 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 5.9e-04
Identity = 18/33 (54.55%), Postives = 25/33 (75.76%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDG 55
           DI IS E+G+HGD++PFD   G +A AF+P +G
Sbjct: 166 DIMISFETGDHGDSYPFDGPRGTLAHAFAPGEG 198

[Close]

BLAST of CmaCh04G027340 vs. ExPASy TrEMBL
Match: A0A0A0KS47 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.0e-06
Identity = 28/42 (66.67%), Postives = 32/42 (76.19%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMALSH 64
           DIKIS E G+HGD  PFDSVGGV+A AF+PTDGR    A+ H
Sbjct: 209 DIKISFEKGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEH 250

[Close]

BLAST of CmaCh04G027340 vs. ExPASy TrEMBL
Match: A0A5A7T7T1 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00140 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.7e-06
Identity = 27/42 (64.29%), Postives = 32/42 (76.19%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMALSH 64
           DIK+S E G+HGD  PFDSVGGV+A AF+PTDGR    A+ H
Sbjct: 178 DIKVSFERGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEH 219

[Close]

BLAST of CmaCh04G027340 vs. ExPASy TrEMBL
Match: A0A1S3C925 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498039 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.8e-06
Identity = 26/42 (61.90%), Postives = 32/42 (76.19%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMALSH 64
           DIK++ E G+HGD  PFDSVGGV+A AF+PTDGR    A+ H
Sbjct: 178 DIKVTFERGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEH 219

[Close]

BLAST of CmaCh04G027340 vs. ExPASy TrEMBL
Match: A0A6J1JQE4 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487397 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 6.5e-06
Identity = 27/39 (69.23%), Postives = 31/39 (79.49%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMA 61
           DIKI  E+G+HGD +PFD VGGVIA AFSP DGR+ L A
Sbjct: 212 DIKIGFETGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDA 250

[Close]

BLAST of CmaCh04G027340 vs. ExPASy TrEMBL
Match: A0A6J1HJ41 (metalloendoproteinase 3-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111464924 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 6.5e-06
Identity = 27/39 (69.23%), Postives = 31/39 (79.49%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMA 61
           DIKI  E+G+HGD +PFD VGGVIA AFSP DGR+ L A
Sbjct: 212 DIKIGFETGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDA 250

[Close]

BLAST of CmaCh04G027340 vs. NCBI nr
Match: XP_038891009.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 63.9 bits (154), Expect = 1.2e-06
Identity = 29/44 (65.91%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 20  SIDIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMALSH 64
           S DIKIS E G+HGD  PFDSVGGV+A AF+PTDGR    A+ H
Sbjct: 212 SADIKISFERGDHGDGSPFDSVGGVLAHAFAPTDGRLHFDAVEH 255

[Close]

BLAST of CmaCh04G027340 vs. NCBI nr
Match: XP_038889209.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 63.2 bits (152), Expect = 2.1e-06
Identity = 28/34 (82.35%), Postives = 29/34 (85.29%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGR 56
           DIKIS ESG+HGD H FD VGGVIA AFSPTDGR
Sbjct: 210 DIKISFESGDHGDGHAFDGVGGVIAHAFSPTDGR 243

[Close]

BLAST of CmaCh04G027340 vs. NCBI nr
Match: KAE8648906.1 (hypothetical protein Csa_009291 [Cucumis sativus])

HSP 1 Score: 63.2 bits (152), Expect = 2.1e-06
Identity = 28/42 (66.67%), Postives = 32/42 (76.19%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMALSH 64
           DIKIS E G+HGD  PFDSVGGV+A AF+PTDGR    A+ H
Sbjct: 154 DIKISFEKGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEH 195

[Close]

BLAST of CmaCh04G027340 vs. NCBI nr
Match: KAG5535004.1 (hypothetical protein RHGRI_022944 [Rhododendron griersonianum])

HSP 1 Score: 63.2 bits (152), Expect = 2.1e-06
Identity = 40/103 (38.83%), Postives = 53/103 (51.46%), Query Frame = 0


Query: 20  SIDIKISLESGEHGDA--HPFDSVGGVIARAFSPTDGRYILMALSHGLLLPFLIRLIWRP 79
           S ++KI     +HGD   + FD  GG +  AF PT+GR+   A     +      LIWR 
Sbjct: 91  SANLKIVFGRLDHGDGIQNAFDGPGGTVPHAFPPTNGRFHYDADESWSVGG--TPLIWRL 150

Query: 80  WRCMRLDTLWAFTTAQWKGVLCCRPSWEELPRVCMPTTPKAIK 121
           W CMRL T      AQ+  + C R    ELP+VCM TT + +K
Sbjct: 151 WLCMRLGTSLGLGIAQFGKLSCTRMLLLELPKVCMETTYRGLK 191

[Close]

BLAST of CmaCh04G027340 vs. NCBI nr
Match: XP_004142457.2 (metalloendoproteinase 3-MMP [Cucumis sativus])

HSP 1 Score: 63.2 bits (152), Expect = 2.1e-06
Identity = 28/42 (66.67%), Postives = 32/42 (76.19%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMALSH 64
           DIKIS E G+HGD  PFDSVGGV+A AF+PTDGR    A+ H
Sbjct: 212 DIKISFEKGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEH 253

[Close]

BLAST of CmaCh04G027340 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.4e-05
Identity = 20/34 (58.82%), Postives = 24/34 (70.59%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGR 56
           DIKI   +G+HGD  PFD V GV+A  FSP +GR
Sbjct: 189 DIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGR 222

[Close]

BLAST of CmaCh04G027340 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 45.1 bits (105), Expect = 5.5e-05
Identity = 20/39 (51.28%), Postives = 25/39 (64.10%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMA 61
           DI I   +G+HGD  PFD V G +A AFSP  G++ L A
Sbjct: 214 DITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDA 252

[Close]

BLAST of CmaCh04G027340 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 44.3 bits (103), Expect = 9.3e-05
Identity = 20/39 (51.28%), Postives = 26/39 (66.67%), Query Frame = 0


Query: 22  DIKISLESGEHGDAHPFDSVGGVIARAFSPTDGRYILMA 61
           D+KI   +G+HGD  PFD V G +A AF+P +GR  L A
Sbjct: 211 DLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDA 249

The following BLAST results are available for this feature:

BLAST of CmaCh04G027340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GWW6	2.0e-04	58.82	Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1	[more]
P29136	2.0e-04	55.88	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
O18767	5.9e-04	54.55	Matrix metalloproteinase-20 OS=Bos taurus OX=9913 GN=MMP20 PE=1 SV=1	[more]
P57748	5.9e-04	54.55	Matrix metalloproteinase-20 OS=Mus musculus OX=10090 GN=Mmp20 PE=2 SV=1	[more]
P79287	5.9e-04	54.55	Matrix metalloproteinase-20 OS=Sus scrofa OX=9823 GN=MMP20 PE=2 SV=1	[more]

BLAST of CmaCh04G027340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KS47	1.0e-06	66.67	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 S...	[more]
A0A5A7T7T1	1.7e-06	64.29	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C925	3.8e-06	61.90	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498039 PE=3 SV...	[more]
A0A6J1JQE4	6.5e-06	69.23	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487397 PE=...	[more]
A0A6J1HJ41	6.5e-06	69.23	metalloendoproteinase 3-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111464924 P...	[more]

BLAST of CmaCh04G027340 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891009.1	1.2e-06	65.91	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]
XP_038889209.1	2.1e-06	82.35	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]
KAE8648906.1	2.1e-06	66.67	hypothetical protein Csa_009291 [Cucumis sativus]	[more]
KAG5535004.1	2.1e-06	38.83	hypothetical protein RHGRI_022944 [Rhododendron griersonianum]	[more]
XP_004142457.2	2.1e-06	66.67	metalloendoproteinase 3-MMP [Cucumis sativus]	[more]

BLAST of CmaCh04G027340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45040.1	1.4e-05	58.82	Matrixin family protein	[more]
AT1G70170.1	5.5e-05	51.28	matrix metalloproteinase	[more]
AT4G16640.1	9.3e-05	51.28	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 9..63 e-value: 6.9E-6 score: 27.6
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 20..54 e-value: 2.0E-5 score: 24.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G027340.1	CmaCh04G027340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity