CmaCh04G027160 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G027160
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPotassium transporter
LocationCma_Chr04: 18329852 .. 18338611 (+)
RNA-Seq ExpressionCmaCh04G027160
SyntenyCmaCh04G027160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCCGAACTTGATCTGGGGTTATCCTGAAAAGGTATTTTCATCTGTTATTTCAACAGATGGTTCTAGAAATCTGATCTTCTTGGCTTTATTTTGTTGCAGAGGGATTCATGGAGGACAATTTTCACTTTGGCTTATCAGGCTTTGGGGGTTGTTTATGGGGATTTGAGCACTTCTCCGCTGTATGTGTACAAAAGCACTTTTGCAAAAAACCTTCAGCACTCTGAGACGAATGAGGAGGTTTATGGGGTTCTTTGCTTTGTGTTTTGGACTTTGACACTTATCCCTTTACTTAAATATGTGTTCATAGTTCTTAAAGCTGATGATAATGGCGAAGGAGGAACTTTTGCTCTGTATTCACTGCTCTGCCGCCATGCTCGCCTTAGCTCCCTTCCAAATCAACAGCTTGCAGATGAGGAGCTTTCTGCTTATAAAAGAGATACACCTGAACCCACTAGTAATTTTAGTTTCAGTTCTTGTTTGAAAGCAACTCTGGAGAAATGTAAAGTTTTGCAGAGAATCTTGCTTGTTCTTGCCTTGATTGGGACTTGCATGGTTATCGGTGATGGAGTTCTCACACCAGCAATTTCAGGTGGCTGATTTGGTTTTTATGTTTTATTAGATGAAAAAATGTTATTCTAAGTTGGGAGGTTTTTGTTTTTTGTTCATATTGTGCAGTCTTTTCAGCTGTGTCAGGGGTTGAGTTATCCATGGCTAAGGAACATCACCAATGTAAGTTTCTTTTTCAGTTCATCCCTGAATTTTGTGATACCTTGATGGATCTGCTTCTACTGTTCCAATGGAGGCCAATTGATCTGTATTAGCTTTTGTATTATCTTTGTATCAACATGTGAGATCTCACATCGGTTAGAGAGGGAAACGAAACATTCTTTATAAAAATATGGAAATCTCTCCCTAGTAGACGCGTTTTAAAATCGTGAGGCCAACAGTGATACGTAACGAGCTAAAACAGACAATACCTGCTAGCGATGGGCTTGAACTGTTGCAAACGGTATCAGAGCTAGCCAGCCCCCAGGGGGTGGATTGTGAGATCTCACATAAGCTGGAGAGGGGAACAAAGTATTTTTTATAAGGGCGTGGAAGCCTCTCCCTAGTAGACGCGTTTTAAAACTGTGAGGCTAACGACGATACGTAACGAACTAAAGCGGACAATATCTGCTAGTGGTGGGCTTGAGTTGTTGCAAATGGTATCAGAGCCAGCCAATGAGGACGCTGGGCCCCTAAGGGGTGGATTGTGAGATCCCACATCGGCTGGAGAGGGGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAATCGTGAGGTTGACGACGATACGTAACGAGCCAAAGCGGACAATATCTGTTAGTGGTGAGCTTGAGCTATTACATTAAAATTTTAGATTCGATATCTTCTCAAATGCGAGGAAGTCAATGAGTAGAATTGCTGGTTTGGTTAGATATAGTCTTGAATAAGATGATCAAATTTGTAAAATGGTTTGGTGGAGTCGGCTAGTCTCTTGCTTTTGTCGTACTTTTTGGTAGCAACTCTTGAATGCTCATAAACATTGCACAAATGTAGGCAAGATTTTGATCATATGAAACAAAATTCCTGTTCTTATTGCCTTGTGATTTTGTTCTCTCATCTTTCCTTGTAGTAGCATTTACTTGTAACTTTTTTTGGTGTTTATTTCGAAAGAATGTTCCGTTCTGTTGCAGATATTAAAGTTCCTCTGGCATGTGCCATTTTGGTATTCTTGTTTGCATTACAACATTACGGCACGCATCGGGTCGGGTTCCTGTTTGCTCCAGTTGTAATTGTATGGCTTCTATGCATCAGTGCCATTGGTTTGTACAATATCTTCTATTGGAATCCTCTTGTTTACAAAGCACTCTCCCCATATTACATGTATAAGTTCTTGAAAAAAACCAAAAAGCAAGGTTGGATGGCGTTGGGCGGAGTCCTCTTGTGTATAACGGGTACGATGATGTTAGATCCAACCGCAATCAATTCTTTACGTCTGTTTCCTTTGTTTCTGATATGTTCGATTATGATTTTCAGGCTCGGAAGCTATGTTTGCTGATCTTGGACACTTCTCACATTTTTCTATCAAGGTACTAATCTGAAGCAAAACAATCCACGATAATATGATATTGTCTACTTTGAGTATAATCCCCCGAGGCTTTACTTTTGGCTATTCCATTGCCCTCATACCAATACAGATAGTGTTCCTTACTTATAAACCCTCGATCTTCCTCTTAATTAGCTAATGTGGGACTACTACTCTCAATAATCCTCAACAATCCTCCCCTCGAACAAAGTACACCATATAGCCTCCTCTGAGACCTCCTTCTCTAGAGCTCTCGAACAGCCTCCCCTTAATCGATACTCAACTCCTTCTTTGGAGTCCTTGAACAAAGTACACCCTTTGTTCGACACTTGAGACGCTCGACTCCTTCTGTCGAACAAAGTACACCCTTTATTCGATACTTAAGTCACTTTTGACTACACCTTCGAGACTCACAACTTCTTGGTTTGACACTTGAGGATTCTATTGACATTGCTAAGCTAATCGCATGACTCTGATACCATGTTAGGAACCATGATCTTCCACAATGGTATGATATTATCCACTTTAAGCATAAGCTCTAAAGGCTTTGCTTTTGGTTCTCCCAAAAGGCCCCATACCAATGGAGACACAGTGTTACTAACTTATAAACCCATAATCTTCCTCTTAATTAGCCAAGGTAGGACTACTCCTCTCAATAATCCTCAACACTCTTTAGCTTTTCAGGTTACGAGCACAATATTTCAACGTTTTCGTAACAATAAGTCACAATTCTGCAGCTTGCTTTCACTTTTGTGGTTTATCCATCCTTAGTTCTTGCATATATGGGACAAGCTGCTTATCTTTCTAAGCATCACAATCTCGACAACGATTATCGAATCGGATTCTATGTTTCTGTTCCTGGTAGGTTGGTTTTAGTATTACCTTTCTTTTTAGTTGAACCAAATAGGCCAATATGAAGTAATGTAAGGAGCTGCCTACGTCTTTTTGCAGAGAAAGTAAGATGGCCTGTTCTTGCAATAGCAATACTTGCAGCAGTTGTGGGCAGTCAAGCAATCATCACGGGCACGTTTTCGATCATCAAACAATGTTCTTCTTTGGGTTGTTTTCCAAGAGTAAAGATCGTTCATACGTCTTCGAAAATACATGGCCAGATTTACATTCCCCTCATCAACTGGATACTAATGATATTATGTTTAGCTGTTACCATTGGATTTCGAGAGACAGAACGCTTGGGCAATGCAGCAGGTATTTGTTCATTGCTAATACTTTTTATCATCTGTTTCTTGAACTTAACGACAAGTCGGTCTGTTTGTTTATCATTCAGGGTTGGCAGTTATTACCGTTATGCTAGTCTCGACGTGCCTAATGTCACTTGTTATTGTGTTATGTTGGCATAAAAGTGTCCTCCTAGCCATAGCCTTCCTAGTCTTCTCTGGCTCCATAGAAGCTCTCTACTTTTCTGCCTCTCTTATGAAGTTCTTTGAAGGGGCTTGGGTTCCTATTGCACTTTCACTGACATTCCTTGTGGTTATGTATGTTTGGCACTATGGCACTCTAAAGAAATATGAGGCTGATGTTGAAAACAAAGTCCCTATAAATTGGCTCCTTGGTATAGGTCCCAAAATCGGGATCGTTCGGGTTCGGGGGATCGGCCTTGTACATACCGAACTCGTTTCAGGGATCCCTTCCATTTTCTCTCATTTTGTCACAAACATTCCAGCCTTCCACGAAATTCTAGTCTTCCTCTGCATCAAATCTGTCACAATGCCTCATGTGAGGCCTGAGGAAAGGTTCTTGGTAGGAAGAGTTGGGCCGAAGGAGTATCGACTCTACCGCTGCATTGCACGATATGGCTACCGTGATGTGCACAAGGATGACTTGGAATTCGAGAGAGACCTCGCCTGCAGCATTGCAGAATTCATCCGATCCGAGAAAACAGAATGCAACACTTTCAGACACAATGACTTGGATGACAATGACGACAACGAAAGAATGAGAGTGATTGGGACTTCATCAACTCAACTAAACGGTATACAAATGTGTGAAAACGAATCATGTTCTTTGCCGATCATCGACAGTTCAGAGATAAGGTCGGAGAGGTTGAGGAGGAAAAGAGTAAGGTTCGTTGTACCCGAAAGTACGAAAATGGATGTCGATACGAGGAGAGAGCTGCAAGAGTTGATGGAAGCAAGGGAGGCAGGAATGGCATTCATAATGGGACATTCATACGTGAAGGCAAAGAAAGGATCAGGTTGGATGAAGAAGGTTGTGCTTAATTATGGGTATGATTTCTTGAGGAAAAACTCAAGAGGCCCATCTTATGCTTTGAGTATTCCTAATGCTTCTACTCTTGAGGTTGGTGTGGTTTATTATGTTTAAAAAATTGTAGTTTTGTGTATTCTTTTTTAAATATGAAACAATTATGAGCGGTTGAATTTAGCCCTAATTTGAAAGTTTAGGAACTTATTAAATATATTTTAGTGTCTTAATTAGATTAATTACATAAATTTTATAATTTTTGAAATAAAAATAAAAAAACATGAATTTTGATGGGTATTTAAAGCAATGGATCATGGATTCACAAAACCCAACAAAGAAGGAAATGGATGGTTCATATATATATATATATATATAAATACATACTTTGGTTAAAGTACAATTTGAGCATTAAAGATTAATTAATAGATTTTTCAAAGAAAACATTCTTTGAGAGAGAAAGATTAGAGGTGTATTACTGTTATTAATATTATTCCACACAATAATTGAGATCTGAAATGGAAAGGCACGTGATGGGCTGAGGACAACAAATCATTAAAAAACAAAAAACATTTTTCTAACATTTTTATTAAATTGATATCCTAATTACACTCTAAGTTTAGTTTGATCTAATAATATAATACTTGAATCGAATTGGATGAGTTTATTCAAATTTTTGTATGATATGGCCAATCAGATCGATTTTTCATTGAATGTTTGAATCGTCCCCTCATAACAAAGCTGAAGTTTACAAAAACTTGATTTTGAAGTTTTACGGGTTTTGGACCAAAAGCACAGGGAATGTTTCTTTATGTAGTCTTCTACCTTCTTTCTCTTTGTCCGAAAAAGTGATTTCTTAGGTTATTTTTTTAATAAAATTGAATTATATTCGAGTTGATGTTGATGGTAATGGATTAATATGTTTGGGTGTGTGCTGGCTGGTTGGTCTCACTCTACGCTCTACTGGCCCTTCGGCTCTGTTTCCGCCTTGGTTTTGTTTTGGGTTTTGGCCTTACTACCTCAATTTCTTTTTCACTCATTGGGACAGCTCTATCTCGTCAATTGCATCTTCTAAAACCACTAAACCTTCTTTGTCTCTTTCACTGTCATTCCTTCTGAGAAAAGGGTACCACATGGTTGATATGTAGAGGCCAAAACCGGTCAACTTTCTTTTCCTCTTTACTCCACTCTTCCTCTATATGTGTACCTTCAATCCCCCAAGTTTTTGAGGGTTTTCCCTCCTCAATCTTCCTCATTCTCGACAATGGCCAGGATTTCCCTCGCTTTTGCTCTCGTTTTTCTTCTTCTTTTTGGTGGGGTTTCTCCTGCTTCTGCTTCGCCTCCTGGAAGTAAGTGTATTTGATGATTATGATGACGATGAGGAAGAAGTAATTTTGAGAAGAACCCATGTTCTTAATCTTATGATTTTTGCATTTTCCCCCTCTTTAAATGCTGTCTATGGCTCACTCAGCTTACGGCATTTCCACTTTTTGTGCAGAAATTGTTCGTGGGGTTGTTTCCAATGTGGTTTCTTCTCTTGTGAAGAAGTTATGGTCGATGAAATCCACTGCCAAAACTGGTATGGATCTTTCTTAGCTGCCTTGGATAGGAAGTGGTCTGTAATTTTTTAACTGGGTTTTCTGCTTAATCTTCTCAGCTGTTTCCAGCCGTTCGATGATGAAATTTGAGAGTGGATATACGGTTGAGACGGTGTTCGATGGCAGTAAGCTGGGAATCGATCCTTATTCGGTCGAGATGTCGCCTAGTGGGGAGCTGCTGATCTTAGATGCTGAAAACAGTAACATCTATAAGATCTCAATGCCATTGTCTCGATGTAAGCATCCTTTTGGGTCACCATTTTTATGCCACATGATGAACATTGAGCTCATTATTTTTATGACACATGATTGGAAGTTTCAAATGGAAGCTTGTGTTTTTACTTTCTTGAGCTCTTGATTATTTTTTCTAATAGATTATTGTACTTTTGTTTTGAAGAAGGATCCACTTTTTGGAGTACGATGTACTTTTCTTAGTTCTGTCTCTTCTTGGCACTAGATGGTATCAGTTTTGGTCCTTTATTTCTTCCAAGCTTTGTTAGGCTAAAAAGAGAAGGCTATTCACCAAAAGTTGTGGCATACATTGGTCATTATCTGAATAAGACTTGGTAGTATGTTTTATGAAAGGATCTGTATGCATTACCTATACTTTTCTGATGAATTTGCTGCAATCTCCTCTACTGGGTGATGTGCAGATAGCAGACCAAAACTGGTTTCAGGATCTGCAGAAGGATACCCCGGACATGTTGATGGCCATCCAAGAGAAGCGAGGATGAATCATCCGAAAGGGCTTACTTTGGATGAAAGGGGAAATATATACATTGCAGATACAATGAATATGGCTATCAGGAAGATTAGTGACACTGGTATATCTACAAATAATCAGGATAGTTCGATATTCGATATTCGATTTTGTAGTAGTATCCAAATTAATATCTGTCTGGTTTAAATGTCTACGCGCATGGAACTCCTATCAGTGTTGAGTTCCATAGGGTGAAAACTCTTATATATCTCAAATAACTGCAGGGGTTACAACTATTGCTGGTGGAAGATGGAACCAAGGGAGTGGTCATATTGATGGTCCTAGTGAAGATGCAAAGTTCTCAAATGATTTTGATGTTGTTTATGTGGGAAGTAGCTGCTCTCTTTTGGTCATAGACAGAGGAAACAAGGCGATTCGAGAGATTGAACTTAACTACGACGACTGCAATACTCAATACGCTGATAGTCTCAACTTGGGTAGAACGAAAAACCTTACTCCTGCTATGCTCTTCATCTCTGCTATTTCTTCTTATGGTAACATGAAAAGTGTACAATCTCTATCTGCAGGGGTAGTGTTGTTGGTCGCTGCTGGTTTGTTCGGCTATCTGCTTGCTCTGCTCCAACGTAGAGTGCAAGCAGTGTTTTCTTCACCAAAGGTAGATCATTCCACAAAATTCATATTTTTTTCTCGTTTCAAAGAAATAACTATGTTGTCTCAAATATACATACATTGTTATGAACATCTGGGTGCTGCCATTTTGCGGATGGTAGTGGGTTGTGATTTTGTAAGTCTGATATTGTCCTCTTTGGGCAGATTTCCCTTTTGAGCTTTCCCTCAAGGTTTTTAAACACGTCTGTTAGGGAAAGTCCAAAGAGGACAATATCTGGTAGTGGTGGGCTTGAGTTGTTATAAATGATGTTAGAGCTAGACACAAGGCGGTGTGTCAACGAGGACTCTAGGGTCCCAAAGGAAGTGGATTGTAAGATTCCACATTGGTTGGAGAGGGGAATGAAATATTTCTTATAAGGGTATGGAAATCTCTCCCTAATAGACGCGTTTTAAAACCTCGAGGGAACCCATAAGAGAAAGCCGAAAGAGGACAATATGTATTAGCGGTGGGCTTAGACTGTTACAATAATGGTGTCAAAGCTTCTTGGAATTGGACTCTCGTTTCTGTTATGTTACAAATAGCTACTTTTCCTTCTGTCTTTGTGTTAAGAAAATTAATTTGATTGGAAATCTTCATTTGGATGAAAATGAAATGGACTCTTAATGCTTCGTGTTTGGATGGTAGGATGAGGATGTAAAAAGTCATCAAATGAAGAAGGCTGCACAAGCTGCCCAATATCAAAGACCTCCTCTCAAGTCAGTCAGGCCTTCCTTGATTCCGAGTGAAGATGAACCAGAGAAACTGGAGGAAGGATTCTTCGGTTCGCTTGGGAGGCTTTTCGTCAACAGTGGCTCGTCTGTAGCCGATATTGTTGGGGGATTTTTGCCTGGATTTCGAAGGAAGCCTCTCAATAACCAGATCCATCAGCAGTTTCAACAAGTCAACAGACATCCAAACTCTTGGCCCTTGCAAGAAAGTTTTGTGATTCCAGATGAAGACGAGCCACCATCCATTGAAACGAAAACTCCGACAATAAAAAAAACGTATCCATTTATGACACAAGACCTCGATCGATCTCATCAGTTCAAGCCAAACAGAAACTACTTCAATGGGTGGGACAATGGTGGTGAATTCCATCAACAGCAGCAACAGCCGCAGCAGATACATCATCAACAGCAGCAGATACAGCAGCAGCCGCAGCAGCAACAAATACAGCAGCAGCAGCAGCAGATGCAACATCATCAGCAGCATCATTATCAGCAATATCAGCAATATCACCATCGACAATACTCTGCAGGCCCGACAACATACTACGAGAAGAATTGCGAGACGAAAGAAATTGTGTTTGGTGCAGTTCAAGAACAAGATGGACGCCGTGAAGCTATGGTGATCAAGGCTGTTGATTATGGAGATCCAAGATATAACCATCACAACATTCGAGCTCGGTACAACTATACTGGTAATCCCAACTCTTATTGA

mRNA sequence

ATGGATCCGAACTTGATCTGGGGTTATCCTGAAAAGAGGGATTCATGGAGGACAATTTTCACTTTGGCTTATCAGGCTTTGGGGGTTGTTTATGGGGATTTGAGCACTTCTCCGCTGTATGTGTACAAAAGCACTTTTGCAAAAAACCTTCAGCACTCTGAGACGAATGAGGAGGTTTATGGGGTTCTTTGCTTTGTGTTTTGGACTTTGACACTTATCCCTTTACTTAAATATGTGTTCATAGTTCTTAAAGCTGATGATAATGGCGAAGGAGGAACTTTTGCTCTGTATTCACTGCTCTGCCGCCATGCTCGCCTTAGCTCCCTTCCAAATCAACAGCTTGCAGATGAGGAGCTTTCTGCTTATAAAAGAGATACACCTGAACCCACTAGTAATTTTAGTTTCAGTTCTTGTTTGAAAGCAACTCTGGAGAAATGTAAAGTTTTGCAGAGAATCTTGCTTGTTCTTGCCTTGATTGGGACTTGCATGGTTATCGGTGATGGAGTTCTCACACCAGCAATTTCAGTCTTTTCAGCTGTGTCAGGGGTTGAGTTATCCATGGCTAAGGAACATCACCAATATATTAAAGTTCCTCTGGCATGTGCCATTTTGGTATTCTTGTTTGCATTACAACATTACGGCACGCATCGGGTCGGGTTCCTGTTTGCTCCAGTTGTAATTGTATGGCTTCTATGCATCAGTGCCATTGGTTTGTACAATATCTTCTATTGGAATCCTCTTGTTTACAAAGCACTCTCCCCATATTACATGTATAAGTTCTTGAAAAAAACCAAAAAGCAAGGTTGGATGGCGTTGGGCGGAGTCCTCTTGTGTATAACGGGCTCGGAAGCTATGTTTGCTGATCTTGGACACTTCTCACATTTTTCTATCAAGCTTGCTTTCACTTTTGTGGTTTATCCATCCTTAGTTCTTGCATATATGGGACAAGCTGCTTATCTTTCTAAGCATCACAATCTCGACAACGATTATCGAATCGGATTCTATGTTTCTGTTCCTGAGAAAGTAAGATGGCCTGTTCTTGCAATAGCAATACTTGCAGCAGTTGTGGGCAGTCAAGCAATCATCACGGGCACGTTTTCGATCATCAAACAATGTTCTTCTTTGGGTTGTTTTCCAAGAGTAAAGATCGTTCATACGTCTTCGAAAATACATGGCCAGATTTACATTCCCCTCATCAACTGGATACTAATGATATTATGTTTAGCTGTTACCATTGGATTTCGAGAGACAGAACGCTTGGGCAATGCAGCAGGGTTGGCAGTTATTACCGTTATGCTAGTCTCGACGTGCCTAATGTCACTTGTTATTGTGTTATGTTGGCATAAAAGTGTCCTCCTAGCCATAGCCTTCCTAGTCTTCTCTGGCTCCATAGAAGCTCTCTACTTTTCTGCCTCTCTTATGAAGTTCTTTGAAGGGGCTTGGGTTCCTATTGCACTTTCACTGACATTCCTTGTGGTTATGTATGTTTGGCACTATGGCACTCTAAAGAAATATGAGGCTGATGTTGAAAACAAAGTCCCTATAAATTGGCTCCTTGGTATAGGTCCCAAAATCGGGATCGTTCGGGTTCGGGGGATCGGCCTTGTACATACCGAACTCGTTTCAGGGATCCCTTCCATTTTCTCTCATTTTGTCACAAACATTCCAGCCTTCCACGAAATTCTAGTCTTCCTCTGCATCAAATCTGTCACAATGCCTCATGTGAGGCCTGAGGAAAGGTTCTTGGTAGGAAGAGTTGGGCCGAAGGAGTATCGACTCTACCGCTGCATTGCACGATATGGCTACCGTGATGTGCACAAGGATGACTTGGAATTCGAGAGAGACCTCGCCTGCAGCATTGCAGAATTCATCCGATCCGAGAAAACAGAATGCAACACTTTCAGACACAATGACTTGGATGACAATGACGACAACGAAAGAATGAGAGTGATTGGGACTTCATCAACTCAACTAAACGGTATACAAATGTGTGAAAACGAATCATGTTCTTTGCCGATCATCGACAGTTCAGAGATAAGGTCGGAGAGGTTGAGGAGGAAAAGAGTAAGGTTCGTTGTACCCGAAAGTACGAAAATGGATGTCGATACGAGGAGAGAGCTGCAAGAGTTGATGGAAGCAAGGGAGGCAGGAATGGCATTCATAATGGGACATTCATACGTGAAGGCAAAGAAAGGATCAGAGGCCAAAACCGGTCAACTTTCTTTTCCTCTTTACTCCACTCTTCCTCTATATGTGTACCTTCAATCCCCCAAGTTTTTGAGGGTTTTCCCTCCTCAATCTTCCTCATTCTCGACAATGGCCAGGATTTCCCTCGCTTTTGCTCTCGTTTTTCTTCTTCTTTTTGGTGGGGTTTCTCCTGCTTCTGCTTCGCCTCCTGGAAAAATTGTTCGTGGGGTTGTTTCCAATGTGGTTTCTTCTCTTGTGAAGAAGTTATGGTCGATGAAATCCACTGCCAAAACTGCTGTTTCCAGCCGTTCGATGATGAAATTTGAGAGTGGATATACGGTTGAGACGGTGTTCGATGGCAGTAAGCTGGGAATCGATCCTTATTCGGTCGAGATGTCGCCTAGTGGGGAGCTGCTGATCTTAGATGCTGAAAACAGTAACATCTATAAGATCTCAATGCCATTGTCTCGATATAGCAGACCAAAACTGGTTTCAGGATCTGCAGAAGGATACCCCGGACATGTTGATGGCCATCCAAGAGAAGCGAGGATGAATCATCCGAAAGGGCTTACTTTGGATGAAAGGGGAAATATATACATTGCAGATACAATGAATATGGCTATCAGGAAGATTAGTGACACTGGGGTTACAACTATTGCTGGTGGAAGATGGAACCAAGGGAGTGGTCATATTGATGGTCCTAGTGAAGATGCAAAGTTCTCAAATGATTTTGATGTTGTTTATGTGGGAAGTAGCTGCTCTCTTTTGGTCATAGACAGAGGAAACAAGGCGATTCGAGAGATTGAACTTAACTACGACGACTGCAATACTCAATACGCTGATAGTCTCAACTTGGGGGTAGTGTTGTTGGTCGCTGCTGGTTTGTTCGGCTATCTGCTTGCTCTGCTCCAACGTAGAGTGCAAGCAGTGTTTTCTTCACCAAAGGATGAGGATGTAAAAAGTCATCAAATGAAGAAGGCTGCACAAGCTGCCCAATATCAAAGACCTCCTCTCAAGTCAGTCAGGCCTTCCTTGATTCCGAGTGAAGATGAACCAGAGAAACTGGAGGAAGGATTCTTCGGTTCGCTTGGGAGGCTTTTCGTCAACAGTGGCTCGTCTGTAGCCGATATTGTTGGGGGATTTTTGCCTGGATTTCGAAGGAAGCCTCTCAATAACCAGATCCATCAGCAGTTTCAACAAGTCAACAGACATCCAAACTCTTGGCCCTTGCAAGAAAGTTTTGTGATTCCAGATGAAGACGAGCCACCATCCATTGAAACGAAAACTCCGACAATAAAAAAAACGTATCCATTTATGACACAAGACCTCGATCGATCTCATCAGTTCAAGCCAAACAGAAACTACTTCAATGGGTGGGACAATGGTGGTGAATTCCATCAACAGCAGCAACAGCCGCAGCAGATACATCATCAACAGCAGCAGATACAGCAGCAGCCGCAGCAGCAACAAATACAGCAGCAGCAGCAGCAGATGCAACATCATCAGCAGCATCATTATCAGCAATATCAGCAATATCACCATCGACAATACTCTGCAGGCCCGACAACATACTACGAGAAGAATTGCGAGACGAAAGAAATTGTGTTTGGTGCAGTTCAAGAACAAGATGGACGCCGTGAAGCTATGGTGATCAAGGCTGTTGATTATGGAGATCCAAGATATAACCATCACAACATTCGAGCTCGGTACAACTATACTGGTAATCCCAACTCTTATTGA

Coding sequence (CDS)

ATGGATCCGAACTTGATCTGGGGTTATCCTGAAAAGAGGGATTCATGGAGGACAATTTTCACTTTGGCTTATCAGGCTTTGGGGGTTGTTTATGGGGATTTGAGCACTTCTCCGCTGTATGTGTACAAAAGCACTTTTGCAAAAAACCTTCAGCACTCTGAGACGAATGAGGAGGTTTATGGGGTTCTTTGCTTTGTGTTTTGGACTTTGACACTTATCCCTTTACTTAAATATGTGTTCATAGTTCTTAAAGCTGATGATAATGGCGAAGGAGGAACTTTTGCTCTGTATTCACTGCTCTGCCGCCATGCTCGCCTTAGCTCCCTTCCAAATCAACAGCTTGCAGATGAGGAGCTTTCTGCTTATAAAAGAGATACACCTGAACCCACTAGTAATTTTAGTTTCAGTTCTTGTTTGAAAGCAACTCTGGAGAAATGTAAAGTTTTGCAGAGAATCTTGCTTGTTCTTGCCTTGATTGGGACTTGCATGGTTATCGGTGATGGAGTTCTCACACCAGCAATTTCAGTCTTTTCAGCTGTGTCAGGGGTTGAGTTATCCATGGCTAAGGAACATCACCAATATATTAAAGTTCCTCTGGCATGTGCCATTTTGGTATTCTTGTTTGCATTACAACATTACGGCACGCATCGGGTCGGGTTCCTGTTTGCTCCAGTTGTAATTGTATGGCTTCTATGCATCAGTGCCATTGGTTTGTACAATATCTTCTATTGGAATCCTCTTGTTTACAAAGCACTCTCCCCATATTACATGTATAAGTTCTTGAAAAAAACCAAAAAGCAAGGTTGGATGGCGTTGGGCGGAGTCCTCTTGTGTATAACGGGCTCGGAAGCTATGTTTGCTGATCTTGGACACTTCTCACATTTTTCTATCAAGCTTGCTTTCACTTTTGTGGTTTATCCATCCTTAGTTCTTGCATATATGGGACAAGCTGCTTATCTTTCTAAGCATCACAATCTCGACAACGATTATCGAATCGGATTCTATGTTTCTGTTCCTGAGAAAGTAAGATGGCCTGTTCTTGCAATAGCAATACTTGCAGCAGTTGTGGGCAGTCAAGCAATCATCACGGGCACGTTTTCGATCATCAAACAATGTTCTTCTTTGGGTTGTTTTCCAAGAGTAAAGATCGTTCATACGTCTTCGAAAATACATGGCCAGATTTACATTCCCCTCATCAACTGGATACTAATGATATTATGTTTAGCTGTTACCATTGGATTTCGAGAGACAGAACGCTTGGGCAATGCAGCAGGGTTGGCAGTTATTACCGTTATGCTAGTCTCGACGTGCCTAATGTCACTTGTTATTGTGTTATGTTGGCATAAAAGTGTCCTCCTAGCCATAGCCTTCCTAGTCTTCTCTGGCTCCATAGAAGCTCTCTACTTTTCTGCCTCTCTTATGAAGTTCTTTGAAGGGGCTTGGGTTCCTATTGCACTTTCACTGACATTCCTTGTGGTTATGTATGTTTGGCACTATGGCACTCTAAAGAAATATGAGGCTGATGTTGAAAACAAAGTCCCTATAAATTGGCTCCTTGGTATAGGTCCCAAAATCGGGATCGTTCGGGTTCGGGGGATCGGCCTTGTACATACCGAACTCGTTTCAGGGATCCCTTCCATTTTCTCTCATTTTGTCACAAACATTCCAGCCTTCCACGAAATTCTAGTCTTCCTCTGCATCAAATCTGTCACAATGCCTCATGTGAGGCCTGAGGAAAGGTTCTTGGTAGGAAGAGTTGGGCCGAAGGAGTATCGACTCTACCGCTGCATTGCACGATATGGCTACCGTGATGTGCACAAGGATGACTTGGAATTCGAGAGAGACCTCGCCTGCAGCATTGCAGAATTCATCCGATCCGAGAAAACAGAATGCAACACTTTCAGACACAATGACTTGGATGACAATGACGACAACGAAAGAATGAGAGTGATTGGGACTTCATCAACTCAACTAAACGGTATACAAATGTGTGAAAACGAATCATGTTCTTTGCCGATCATCGACAGTTCAGAGATAAGGTCGGAGAGGTTGAGGAGGAAAAGAGTAAGGTTCGTTGTACCCGAAAGTACGAAAATGGATGTCGATACGAGGAGAGAGCTGCAAGAGTTGATGGAAGCAAGGGAGGCAGGAATGGCATTCATAATGGGACATTCATACGTGAAGGCAAAGAAAGGATCAGAGGCCAAAACCGGTCAACTTTCTTTTCCTCTTTACTCCACTCTTCCTCTATATGTGTACCTTCAATCCCCCAAGTTTTTGAGGGTTTTCCCTCCTCAATCTTCCTCATTCTCGACAATGGCCAGGATTTCCCTCGCTTTTGCTCTCGTTTTTCTTCTTCTTTTTGGTGGGGTTTCTCCTGCTTCTGCTTCGCCTCCTGGAAAAATTGTTCGTGGGGTTGTTTCCAATGTGGTTTCTTCTCTTGTGAAGAAGTTATGGTCGATGAAATCCACTGCCAAAACTGCTGTTTCCAGCCGTTCGATGATGAAATTTGAGAGTGGATATACGGTTGAGACGGTGTTCGATGGCAGTAAGCTGGGAATCGATCCTTATTCGGTCGAGATGTCGCCTAGTGGGGAGCTGCTGATCTTAGATGCTGAAAACAGTAACATCTATAAGATCTCAATGCCATTGTCTCGATATAGCAGACCAAAACTGGTTTCAGGATCTGCAGAAGGATACCCCGGACATGTTGATGGCCATCCAAGAGAAGCGAGGATGAATCATCCGAAAGGGCTTACTTTGGATGAAAGGGGAAATATATACATTGCAGATACAATGAATATGGCTATCAGGAAGATTAGTGACACTGGGGTTACAACTATTGCTGGTGGAAGATGGAACCAAGGGAGTGGTCATATTGATGGTCCTAGTGAAGATGCAAAGTTCTCAAATGATTTTGATGTTGTTTATGTGGGAAGTAGCTGCTCTCTTTTGGTCATAGACAGAGGAAACAAGGCGATTCGAGAGATTGAACTTAACTACGACGACTGCAATACTCAATACGCTGATAGTCTCAACTTGGGGGTAGTGTTGTTGGTCGCTGCTGGTTTGTTCGGCTATCTGCTTGCTCTGCTCCAACGTAGAGTGCAAGCAGTGTTTTCTTCACCAAAGGATGAGGATGTAAAAAGTCATCAAATGAAGAAGGCTGCACAAGCTGCCCAATATCAAAGACCTCCTCTCAAGTCAGTCAGGCCTTCCTTGATTCCGAGTGAAGATGAACCAGAGAAACTGGAGGAAGGATTCTTCGGTTCGCTTGGGAGGCTTTTCGTCAACAGTGGCTCGTCTGTAGCCGATATTGTTGGGGGATTTTTGCCTGGATTTCGAAGGAAGCCTCTCAATAACCAGATCCATCAGCAGTTTCAACAAGTCAACAGACATCCAAACTCTTGGCCCTTGCAAGAAAGTTTTGTGATTCCAGATGAAGACGAGCCACCATCCATTGAAACGAAAACTCCGACAATAAAAAAAACGTATCCATTTATGACACAAGACCTCGATCGATCTCATCAGTTCAAGCCAAACAGAAACTACTTCAATGGGTGGGACAATGGTGGTGAATTCCATCAACAGCAGCAACAGCCGCAGCAGATACATCATCAACAGCAGCAGATACAGCAGCAGCCGCAGCAGCAACAAATACAGCAGCAGCAGCAGCAGATGCAACATCATCAGCAGCATCATTATCAGCAATATCAGCAATATCACCATCGACAATACTCTGCAGGCCCGACAACATACTACGAGAAGAATTGCGAGACGAAAGAAATTGTGTTTGGTGCAGTTCAAGAACAAGATGGACGCCGTGAAGCTATGGTGATCAAGGCTGTTGATTATGGAGATCCAAGATATAACCATCACAACATTCGAGCTCGGTACAACTATACTGGTAATCCCAACTCTTATTGA

Protein sequence

MDPNLIWGYPEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTLTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEPTSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAKEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVYKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCENESCSLPIIDSSEIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIMGHSYVKAKKGSEAKTGQLSFPLYSTLPLYVYLQSPKFLRVFPPQSSSFSTMARISLAFALVFLLLFGGVSPASASPPGKIVRGVVSNVVSSLVKKLWSMKSTAKTAVSSRSMMKFESGYTVETVFDGSKLGIDPYSVEMSPSGELLILDAENSNIYKISMPLSRYSRPKLVSGSAEGYPGHVDGHPREARMNHPKGLTLDERGNIYIADTMNMAIRKISDTGVTTIAGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGSSCSLLVIDRGNKAIREIELNYDDCNTQYADSLNLGVVLLVAAGLFGYLLALLQRRVQAVFSSPKDEDVKSHQMKKAAQAAQYQRPPLKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSGSSVADIVGGFLPGFRRKPLNNQIHQQFQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPTIKKTYPFMTQDLDRSHQFKPNRNYFNGWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQIQQQQQQMQHHQQHHYQQYQQYHHRQYSAGPTTYYEKNCETKEIVFGAVQEQDGRREAMVIKAVDYGDPRYNHHNIRARYNYTGNPNSY
Homology
BLAST of CmaCh04G027160 vs. ExPASy Swiss-Prot
Match: Q8W4I4 (Potassium transporter 6 OS=Arabidopsis thaliana OX=3702 GN=POT6 PE=2 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 1.3e-310
Identity = 530/732 (72.40%), Postives = 637/732 (87.02%), Query Frame = 0

Query: 12  KRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTLT 71
           K++SWRT+ TLAYQ+LGVVYGDLS SPLYVYKSTFA+++ HSE+NEE++GVL F+FWT+T
Sbjct: 12  KKESWRTVLTLAYQSLGVVYGDLSISPLYVYKSTFAEDIHHSESNEEIFGVLSFIFWTIT 71

Query: 72  LIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEPTS 131
           L+PLLKYVFIVL+ADDNGEGGTFALYSLLCRHAR++SLP+ QLADE+L  YK D+   +S
Sbjct: 72  LVPLLKYVFIVLRADDNGEGGTFALYSLLCRHARVNSLPSCQLADEQLIEYKTDSIGSSS 131

Query: 132 --NFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAK 191
                F++ LK+TLEK  VLQ+ILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSM+K
Sbjct: 132 MPQSGFAASLKSTLEKHGVLQKILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMSK 191

Query: 192 EHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVY 251
           EHH+YI++P AC IL+ LFALQHYGTHRVGFLFAPV+++WL+CISAIG+YNIF+WNP VY
Sbjct: 192 EHHKYIELPAACVILIGLFALQHYGTHRVGFLFAPVILLWLMCISAIGVYNIFHWNPHVY 251

Query: 252 KALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSL 311
           +ALSPYYMYKFLKKT+ +GWM+LGG+LLCITGSEAMFADLGHFS  SIK+AFT +VYPSL
Sbjct: 252 QALSPYYMYKFLKKTQSRGWMSLGGILLCITGSEAMFADLGHFSQLSIKIAFTSLVYPSL 311

Query: 312 VLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSII 371
           +LAYMGQAAYLS+HH ++++Y IGFYVSVPE++RWPVL IAILAAVVGSQAIITGTFSII
Sbjct: 312 ILAYMGQAAYLSQHHIIESEYNIGFYVSVPERLRWPVLVIAILAAVVGSQAIITGTFSII 371

Query: 372 KQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVI 431
           KQCS+LGCFP+VKIVHTSSKIHGQIYIP INWILM+LCLAVTIGFR+T+RLGNA+GLAVI
Sbjct: 372 KQCSALGCFPKVKIVHTSSKIHGQIYIPEINWILMVLCLAVTIGFRDTKRLGNASGLAVI 431

Query: 432 TVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLT 491
           TVMLV+TCLMSLVIVLCWHKSV+ AI F+VF G+IE+LYFSASL+KF EGAWVPIAL+  
Sbjct: 432 TVMLVTTCLMSLVIVLCWHKSVIFAIVFVVFFGTIESLYFSASLIKFLEGAWVPIALAFC 491

Query: 492 FLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFS 551
           FL+ M  WHYGTLK+YE DV+NKV +NWLL +   +GI RVRG+GL+HTELVSG+P+IFS
Sbjct: 492 FLLAMCTWHYGTLKRYEYDVQNKVSVNWLLSLSQTLGIARVRGLGLIHTELVSGVPAIFS 551

Query: 552 HFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDD 611
           HFVTN+PAFH++LVFLC+KSV +PHVRP+ERFLVGR+GPKE+R+YRCI R+GYRDVHKDD
Sbjct: 552 HFVTNLPAFHQVLVFLCVKSVPVPHVRPQERFLVGRIGPKEFRIYRCIVRFGYRDVHKDD 611

Query: 612 LEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQ-MCENES 671
            EFE DL CSIAEFIR+E     T       + +D++RM V+GT ST + GI+   E++ 
Sbjct: 612 FEFEGDLVCSIAEFIRTEAETAATAAET---NGEDDDRMSVVGTCSTYMQGIEDHYESDI 671

Query: 672 CSLPIIDSSEIRSERLR-------RKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFI 731
                  +SEIRS + +       +KRVRFVVPE+ K++ +TR+EL EL EARE G+A+I
Sbjct: 672 DDPDKPGTSEIRSPKPKKKSKSKVKKRVRFVVPETPKIEKETRQELMELTEAREGGVAYI 731

Query: 732 MGHSYVKAKKGS 734
           MG++Y+KAK GS
Sbjct: 732 MGNAYMKAKPGS 740

BLAST of CmaCh04G027160 vs. ExPASy Swiss-Prot
Match: Q9M7J9 (Potassium transporter 8 OS=Arabidopsis thaliana OX=3702 GN=POT8 PE=2 SV=2)

HSP 1 Score: 1059.3 bits (2738), Expect = 3.5e-308
Identity = 527/731 (72.09%), Postives = 626/731 (85.64%), Query Frame = 0

Query: 10  PEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWT 69
           P K++SW T+ TLAYQ+LGVVYGDL+TSPLYVYKSTFA+++ HSETNEE++GVL  +FWT
Sbjct: 11  PVKKESWWTVLTLAYQSLGVVYGDLATSPLYVYKSTFAEDITHSETNEEIFGVLSLIFWT 70

Query: 70  LTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEP 129
           LTLIPL+KYVFIVL+ADDNGEGGTFALYSLLCRHAR+SSLPN QLADE+LS YK+++ E 
Sbjct: 71  LTLIPLVKYVFIVLRADDNGEGGTFALYSLLCRHARISSLPNFQLADEDLSEYKKNSGEN 130

Query: 130 TSNFSFSS-CLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMA 189
                     LK TLEK K LQ +LLVLALIGTCMVIGDGVLTPAISVFSAVSG+ELSM+
Sbjct: 131 PMRLKVPGWSLKNTLEKHKFLQNMLLVLALIGTCMVIGDGVLTPAISVFSAVSGLELSMS 190

Query: 190 KEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLV 249
           K+ HQY++VP+ CAIL+ LF+LQHYGTHR+GF+FAP+V+ WLLCIS IG+YNIF+WNP V
Sbjct: 191 KQQHQYVEVPVVCAILILLFSLQHYGTHRLGFVFAPIVLAWLLCISTIGVYNIFHWNPHV 250

Query: 250 YKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPS 309
           YKALSPYY+YKFLKKT+K+GWM+LGG+LLCITGSEAMFADLGHF+  SI++AFTF VYPS
Sbjct: 251 YKALSPYYIYKFLKKTRKRGWMSLGGILLCITGSEAMFADLGHFTQLSIQIAFTFAVYPS 310

Query: 310 LVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSI 369
           L+LAYMGQAAYLSKHH L +DYRIGFYVSVPE++RWPVLAIAILAAVVGSQAIITGTFSI
Sbjct: 311 LILAYMGQAAYLSKHHVLQSDYRIGFYVSVPEQIRWPVLAIAILAAVVGSQAIITGTFSI 370

Query: 370 IKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAV 429
           IKQC+SLGCFP+VKIVHTSS++HGQIYIP INW LM+LCLAVT+GFR+T+ + NA+GLAV
Sbjct: 371 IKQCTSLGCFPKVKIVHTSSRMHGQIYIPEINWTLMLLCLAVTVGFRDTKHISNASGLAV 430

Query: 430 ITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSL 489
           ITVMLV+TCLMSLVIVLCW KS L A+AF+ F G+IE LYFSASL+KF EGAWVP+ALS 
Sbjct: 431 ITVMLVTTCLMSLVIVLCWRKSSLYALAFIFFFGTIEVLYFSASLIKFLEGAWVPVALSF 490

Query: 490 TFLVVMYVWHYGTLKKYEADVENKVPINWLLGI--GPKIGIVRVRGIGLVHTELVSGIPS 549
            FL++MYVWHYGTLK+YE DV+NKV INWLL +     +GIVRV GIG+++TELVSGIP+
Sbjct: 491 IFLLIMYVWHYGTLKRYEFDVQNKVSINWLLTLFGSSNLGIVRVHGIGVINTELVSGIPA 550

Query: 550 IFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVH 609
           IFSHF+TN+PAFH+++VFLC+KSV +PHV+PEERFLVGRVGPKEYRLYRCIARYGYRDVH
Sbjct: 551 IFSHFITNLPAFHQVVVFLCVKSVPVPHVKPEERFLVGRVGPKEYRLYRCIARYGYRDVH 610

Query: 610 KDDLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCEN 669
           KDD+EFE DL CSIAEFIRS+K     +  +  +++  NER+ V+  SS+ L G+Q+ E+
Sbjct: 611 KDDVEFENDLICSIAEFIRSDKP--LNYSPDPENESGINERLTVVAASSSNLEGVQIYED 670

Query: 670 ESCSLPIIDSSE----IRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIM 729
           +        SS     +      +KRVRFV+PES ++D     EL EL EAREAGMAFIM
Sbjct: 671 DGSDKQEPSSSSEVIMVAPSPRFKKRVRFVLPESARIDRSAEEELTELTEAREAGMAFIM 730

Query: 730 GHSYVKAKKGS 734
           GHSYV+AK GS
Sbjct: 731 GHSYVRAKSGS 739

BLAST of CmaCh04G027160 vs. ExPASy Swiss-Prot
Match: Q67VS5 (Potassium transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK10 PE=2 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 7.2e-285
Identity = 510/782 (65.22%), Postives = 604/782 (77.24%), Query Frame = 0

Query: 12  KRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTLT 71
           +R  WR   +LAYQ+LGVVYGDLSTSPLYVYK+ FA+++QHSETNEE+ GVL FVFWTLT
Sbjct: 26  RRLPWRMTMSLAYQSLGVVYGDLSTSPLYVYKAAFAEDIQHSETNEEILGVLSFVFWTLT 85

Query: 72  LIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLP------NQQLADEE--LSAYK 131
           L+PLLKYV +VL+ADDNGEGGTFALYSLLCRHAR + LP        +  DE+  L A  
Sbjct: 86  LVPLLKYVCVVLRADDNGEGGTFALYSLLCRHARAALLPPGGGGGGGEPGDEDQFLDAGA 145

Query: 132 RDTPEPTSNF----------SFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPA 191
                   N             ++ ++  LE+ KVLQR+LLVLAL+GTCMVIGDGVLTPA
Sbjct: 146 DKKAAANGNALALSGRGGGGGAAAGVRRLLERHKVLQRVLLVLALVGTCMVIGDGVLTPA 205

Query: 192 ISVFSAVSGVELSMAKEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCI 251
           ISVFSAVSG+ELSM K  H+Y++VP+AC +LV LF LQHYGTHRVGFLFAP+VI WLLCI
Sbjct: 206 ISVFSAVSGLELSMEKHQHKYVEVPIACFVLVCLFCLQHYGTHRVGFLFAPIVITWLLCI 265

Query: 252 SAIGLYNIFYWNPLVYKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFS 311
           S IG+YNI +W P VY+ALSPYYMYKFLKKT++ GWM+LGG+LLCITGSEAMFADLGHF+
Sbjct: 266 SMIGVYNIVHWEPNVYRALSPYYMYKFLKKTQRGGWMSLGGILLCITGSEAMFADLGHFN 325

Query: 312 HFSIKLAFTFVVYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILA 371
             SI++AFT +VYPSL+LAYMGQAAYL KHH +++DYRIGFYVSVPEK+RWPVLAIAILA
Sbjct: 326 QLSIQIAFTCMVYPSLILAYMGQAAYLCKHHIIESDYRIGFYVSVPEKIRWPVLAIAILA 385

Query: 372 AVVGSQAIITGTFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIG 431
           AVVGSQA+ITGTFS+IKQC++LGCFPRVKIVHTS K+HGQIYIP INWILMILCLA+TIG
Sbjct: 386 AVVGSQAVITGTFSMIKQCTALGCFPRVKIVHTSDKVHGQIYIPEINWILMILCLAITIG 445

Query: 432 FRETERLGNAAGLAVITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASL 491
           FR+T+ LGNA+GLAVITVMLV+TCLMSLVIVLCWHKS+ LA  F++F G+IEALYFSASL
Sbjct: 446 FRDTKHLGNASGLAVITVMLVTTCLMSLVIVLCWHKSIFLAFGFIIFFGTIEALYFSASL 505

Query: 492 MKFFEGAWVPIALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGI 551
           +KF EGAWVPI L+  F+ +M +WHYGT+KKYE D++NKV INWLLG+ P +GIVRVRGI
Sbjct: 506 IKFREGAWVPIVLAFIFMAIMCIWHYGTIKKYEFDLQNKVSINWLLGLSPNLGIVRVRGI 565

Query: 552 GLVHTELVSGIPSIFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRL 611
           GL+HTEL SGIP+IFSHFVTN+PAFH++L+FLCIK+V +PHV PEERFLVGR+GPKEYR+
Sbjct: 566 GLIHTELDSGIPAIFSHFVTNLPAFHQVLIFLCIKNVPIPHVSPEERFLVGRIGPKEYRI 625

Query: 612 YRCIARYGYRDVHKDDLEFERDLACSIAEFIRS-------------EKTECNTFRHNDLD 671
           YRCI RYGY DVHKDD EFE++L CS+AEFIRS              K  C         
Sbjct: 626 YRCIVRYGYHDVHKDDQEFEKELVCSVAEFIRSGAAAAADAAASSKPKNVCGGGAEE--S 685

Query: 672 DNDDNERMRVIGTSSTQLNGIQMCENESCSLPIIDSS--------------------EIR 731
           + ++ ERM VI + S ++    M E+     P  + +                    EI 
Sbjct: 686 EKEEEERMSVIPSGSIRM----MEEDGGAGAPSSEDTVGGSGSGSGRGSSRGGGGAREIM 745

Query: 732 SER--------LRRKRVRFVVP-ESTKMDVDTRRELQELMEAREAGMAFIMGHSYVKAKK 734
           S            RKRVRFV+P  S + D   R ELQELM+AREAGMAFI+GHSYVKAK 
Sbjct: 746 SPSPSPPPVVVAPRKRVRFVLPAASPRPDAGVREELQELMDAREAGMAFILGHSYVKAKS 801

BLAST of CmaCh04G027160 vs. ExPASy Swiss-Prot
Match: Q6YWQ4 (Potassium transporter 25 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK25 PE=2 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 2.8e-281
Identity = 494/725 (68.14%), Postives = 590/725 (81.38%), Query Frame = 0

Query: 13  RDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFA-KNLQHSETNEEVYGVLCFVFWTLT 72
           R+SW     LAYQ+LGVVYGD++TSPLYVYKS FA  ++QHS  NEE+YGVL FVFWTLT
Sbjct: 21  RESWGASLLLAYQSLGVVYGDVATSPLYVYKSAFAGDDIQHSAGNEEIYGVLSFVFWTLT 80

Query: 73  LIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEPTS 132
           LI L+KYV IVL+ADD GEGGTFALYSL+CRH R   LP    A +EL+   R      S
Sbjct: 81  LISLVKYVLIVLRADDGGEGGTFALYSLICRHVRAGLLPGG--AGDELAVGGRRDARAMS 140

Query: 133 NFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAKEH 192
                  L+A LE+ +VLQR+LL+ AL+GTCMVIGDGVLTPA+SV+SAVSG+ELSM  EH
Sbjct: 141 R------LRAMLERYRVLQRLLLLFALLGTCMVIGDGVLTPAVSVYSAVSGLELSMEHEH 200

Query: 193 HQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVYKA 252
           H+Y+++P+ CAIL+ LFALQHYGTHRVGF+FAP+V VWLLCISAIG+YNI +WN  VY+A
Sbjct: 201 HKYVQLPVTCAILIGLFALQHYGTHRVGFIFAPIVCVWLLCISAIGVYNIVHWNHHVYRA 260

Query: 253 LSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSLVL 312
           LSPYYMY+FLKKT+  GWM+LGG+LLC+TGSEAM+ADLGHFS  SIK+AF  VVYP+LVL
Sbjct: 261 LSPYYMYQFLKKTQTGGWMSLGGILLCVTGSEAMYADLGHFSQSSIKIAFMSVVYPALVL 320

Query: 313 AYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSIIKQ 372
           AYMGQAAY+S+HH+ +N Y IGFYVSVPEK+RWPVL IAILAAVVGSQA+ITGTFSIIKQ
Sbjct: 321 AYMGQAAYISQHHSFENAYHIGFYVSVPEKLRWPVLVIAILAAVVGSQAVITGTFSIIKQ 380

Query: 373 CSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVITV 432
           CSSL CFP VKIVHTSS +HGQIYIP INWILMILCLAVT+GFR T+ L NA GLAVITV
Sbjct: 381 CSSLSCFPGVKIVHTSSTVHGQIYIPEINWILMILCLAVTLGFRNTKHLANAQGLAVITV 440

Query: 433 MLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLTFL 492
           MLV+TCLMSLVIVLCW+KS+ LA+ FL+F G+IE LYFSASL+KF EGAWVPI LS  F+
Sbjct: 441 MLVTTCLMSLVIVLCWNKSIFLALGFLIFFGTIEVLYFSASLVKFHEGAWVPITLSFIFM 500

Query: 493 VVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFSHF 552
           +VM VWHYGT+KKYE D +NKV +NWLL +GP +GIVRVRGIGL+HTELVSGIP+IFSHF
Sbjct: 501 IVMCVWHYGTIKKYEFDFQNKVSVNWLLNLGPSLGIVRVRGIGLIHTELVSGIPAIFSHF 560

Query: 553 VTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDLE 612
           VTN+PAFH++LVFLC+KSV +PHV+PEERFLVGR+GPKEYRLYR I RYGYRDV KDD+E
Sbjct: 561 VTNLPAFHQVLVFLCVKSVPVPHVQPEERFLVGRIGPKEYRLYRVIVRYGYRDVQKDDIE 620

Query: 613 FERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQM-CENESCS 672
           FE+DL  SIAEFIRS  +      HN + ++ D    ++   S    NGI +  E+    
Sbjct: 621 FEKDLVSSIAEFIRSGDS-----HHNGVLEDTDKSCEKLSSIS----NGIPLWMEDGEVD 680

Query: 673 LPIIDSSEIRSERL--RRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIMGHSYVK 732
                  E  ++ +   RK+ RFV+P++ ++D + RRELQELM+AREAGM+FI+GHSY+K
Sbjct: 681 ASASPHKETDTQIISPNRKKARFVLPKNAQVDSEVRRELQELMDAREAGMSFILGHSYMK 728

Query: 733 AKKGS 734
           AK GS
Sbjct: 741 AKSGS 728

BLAST of CmaCh04G027160 vs. ExPASy Swiss-Prot
Match: Q5Z6K9 (Potassium transporter 24 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK24 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 5.0e-270
Identity = 482/728 (66.21%), Postives = 580/728 (79.67%), Query Frame = 0

Query: 16  WRTIFTLAYQALGVVYGDLSTSPLYVYKSTFA-KNLQHSETNEEVYGVLCFVFWTLTLIP 75
           WR    LAYQ+LGVVYG+++ +PLYVY+S FA  +++HS  NEE+YG L  VFWTLTL+P
Sbjct: 19  WREEAVLAYQSLGVVYGEVAAAPLYVYRSAFAGGDIEHSAGNEEIYGALSLVFWTLTLVP 78

Query: 76  LLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLP--NQQLADEELSAYKRDTPEPTSN 135
           L KYV +VL+ADD GEGGTFALYSL+CR  R   LP      A EEL A         + 
Sbjct: 79  LAKYVLLVLRADDAGEGGTFALYSLICRRVRAGLLPPCAAAAAGEELDA-------AGAA 138

Query: 136 FSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAKEHH 195
            +  S ++A LE+ +VLQR+LL+LAL+GTCMVIGDGVLTPA+SVFSAVSG+ELSM K+ H
Sbjct: 139 AAPVSAVRAALERHRVLQRLLLLLALLGTCMVIGDGVLTPAVSVFSAVSGLELSMDKDQH 198

Query: 196 QYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVYKAL 255
           +YI +P+ C ILV LFALQHYGTHRVGFLFAP+V +WLLCIS IG+YNI +WNP VY+AL
Sbjct: 199 KYILLPITCVILVCLFALQHYGTHRVGFLFAPIVCLWLLCISIIGVYNIIHWNPHVYQAL 258

Query: 256 SPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSLVLA 315
           SPYYMYKFL+KT+  GWM+LGG+LLC+TGSEAM+ADLGHF+  SIK+AFT +VYP+LVLA
Sbjct: 259 SPYYMYKFLRKTQTGGWMSLGGILLCVTGSEAMYADLGHFTQNSIKMAFTLLVYPALVLA 318

Query: 316 YMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSIIKQC 375
           YMGQAAY+S+HHN ++   IGFYVSVPEK+RWPVL IAILA+VVGSQAIITGTFSIIKQC
Sbjct: 319 YMGQAAYISRHHNFEDGSHIGFYVSVPEKIRWPVLGIAILASVVGSQAIITGTFSIIKQC 378

Query: 376 SSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVITVM 435
           SSL CFPRVKIVHTSS +HGQIYIP INWILMILCL+VTIGFR+T+ L NA GLAVITVM
Sbjct: 379 SSLNCFPRVKIVHTSSTVHGQIYIPEINWILMILCLSVTIGFRDTKHLTNAQGLAVITVM 438

Query: 436 LVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLTFLV 495
           LV+TCLMSLVI+LCW+KS++ A++FL+F G+IE +YF+ASL+KF EGAWVP+ LS  F++
Sbjct: 439 LVTTCLMSLVILLCWNKSIVYALSFLLFFGAIEVIYFAASLVKFHEGAWVPVTLSFIFMM 498

Query: 496 VMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFSHFV 555
           VM VWHYGT KKYE DV+NKV I+WLL IGP +GIVRVRGIGL+HTEL+SGIP+IFSHFV
Sbjct: 499 VMCVWHYGTKKKYEFDVQNKVSISWLLNIGPSLGIVRVRGIGLIHTELMSGIPAIFSHFV 558

Query: 556 TNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDLEF 615
           TN+PAFH++LVFLCIKSV++PHV+PEERFLVGR+GPK+YR+YR I RYGYRDV KDD+EF
Sbjct: 559 TNLPAFHQVLVFLCIKSVSVPHVQPEERFLVGRIGPKKYRIYRVIVRYGYRDVQKDDVEF 618

Query: 616 ERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCENE----- 675
           E+DL  SIAEFIR   +  N+F         D       G S     G+ + E E     
Sbjct: 619 EKDLVSSIAEFIRCADSNQNSFM--------DGASHSCEGLSFIS-KGLPLEEEEGEFDG 678

Query: 676 SCSLPIIDSSEIRSERL--RRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIMGHS 734
           S S       EI       + KRVRF +P+ TK+D + R ELQELMEAREAGM+FI G S
Sbjct: 679 SDSTGSSAHKEINPNTTAPKPKRVRFALPKDTKIDREVRGELQELMEAREAGMSFITGRS 730

BLAST of CmaCh04G027160 vs. ExPASy TrEMBL
Match: A0A498J1T4 (Potassium transporter OS=Malus domestica OX=3750 GN=DVH24_031797 PE=3 SV=1)

HSP 1 Score: 1777.7 bits (4603), Expect = 0.0e+00
Identity = 935/1340 (69.78%), Postives = 1101/1340 (82.16%), Query Frame = 0

Query: 9    YPEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFW 68
            +P K+DSW+T+ TLAYQ+LGVVYGDLSTSPLYVYKSTFA+++ HSETNEE++GVL FVFW
Sbjct: 10   HPIKKDSWKTLLTLAYQSLGVVYGDLSTSPLYVYKSTFAEDIHHSETNEEIFGVLSFVFW 69

Query: 69   TLTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPE 128
            TLTLIPL+KYVFIVL+ADDNGEGGTFALYSLL RHAR+SSLPN QLADEELS Y +D   
Sbjct: 70   TLTLIPLVKYVFIVLRADDNGEGGTFALYSLLSRHARVSSLPNCQLADEELSEYTKDGVV 129

Query: 129  PTSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMA 188
            PT+N +F S LK+TLEK KVL+++LLVLALIGTCMVIGDGVLTPAISVFSAVSG+ELSM+
Sbjct: 130  PTANSAFGSSLKSTLEKHKVLKKVLLVLALIGTCMVIGDGVLTPAISVFSAVSGLELSMS 189

Query: 189  KEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLV 248
            K+ H+Y++VP+AC IL+FLFALQHYGTHRVGFLFAP+VI WLLCIS+IG+YNIF+WNP V
Sbjct: 190  KQQHRYVEVPVACVILIFLFALQHYGTHRVGFLFAPIVITWLLCISSIGVYNIFHWNPHV 249

Query: 249  YKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPS 308
            Y+ALSPYYMYKFLKKT+K GWM+LGG+LLC+TGSEAMFADLGHFS  SIK+AFTFVVYPS
Sbjct: 250  YQALSPYYMYKFLKKTRKGGWMSLGGILLCMTGSEAMFADLGHFSQTSIKIAFTFVVYPS 309

Query: 309  LVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSI 368
            L+LAYMGQAAYLS+HH + +DYRIGFY SVPEK+RWPVLAIAILAAVVGSQAIITGTFSI
Sbjct: 310  LILAYMGQAAYLSRHHIVASDYRIGFYESVPEKIRWPVLAIAILAAVVGSQAIITGTFSI 369

Query: 369  IKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAV 428
            IKQCS+LGCFPRVKI+HTSSKIHGQIYIP INW LM+LCLAVTIGFR+T+ +GNA+GLAV
Sbjct: 370  IKQCSALGCFPRVKIIHTSSKIHGQIYIPEINWTLMLLCLAVTIGFRDTKSMGNASGLAV 429

Query: 429  ITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSL 488
            ITVMLV+TCLMSLVIVLCWHKS+  A+ F++F GS+EALYFSASL+KF EGAWVP+ALS 
Sbjct: 430  ITVMLVTTCLMSLVIVLCWHKSLFWAVCFILFFGSLEALYFSASLIKFREGAWVPVALSF 489

Query: 489  TFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIF 548
             FL+VMYVW YGT+KKYE DV+NKV INWLL +GP +GIVRVRGIGL+HTELVSGIP+IF
Sbjct: 490  IFLMVMYVWQYGTIKKYEFDVQNKVSINWLLSLGPTLGIVRVRGIGLIHTELVSGIPAIF 549

Query: 549  SHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKD 608
            SHFVTN+PAFH+++VFLCIKSV +PHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKD
Sbjct: 550  SHFVTNLPAFHQVVVFLCIKSVPVPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKD 609

Query: 609  DLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCENES 668
            D+EFERDL CSIAEFIRSE+ ECN       +  +D+E+M V+GTSS+ L+GI+M  +E+
Sbjct: 610  DMEFERDLVCSIAEFIRSERPECNL----SPEQLEDDEKMTVVGTSSSNLDGIRMSVDEA 669

Query: 669  CSLPIIDSS---EIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIMGHS 728
                I  +S   EIR     +KRVRFVVPE+ ++D +   ELQELMEAREAGMAFI+GHS
Sbjct: 670  DFSEIASTSELQEIRPTERAKKRVRFVVPETPRIDREAVEELQELMEAREAGMAFILGHS 729

Query: 729  YVKAKKGS-------------------EAKTGQLSFPLYSTLPL-YVY-----LQSP--- 788
            YVKAK+GS                      +  LS P  STL +  VY     +QS    
Sbjct: 730  YVKAKRGSSFMKKIVINFGYDFLRRNFRGPSYALSIPHASTLEVGMVYHSRWAMQSASDE 789

Query: 789  ------------KFLRVFPPQSSSFSTMARISLAFALVFLLLFGGVSP-ASASPPGKIVR 848
                        K++        S ++M R  +A  LVFLLL G  S  ASA+PP KIV 
Sbjct: 790  KVTEHCLWSLLLKWISYKLRPFKSSASMVRNWVALLLVFLLLSGDFSSGASATPPAKIVT 849

Query: 849  GVVSNVVSSLVKKLWSMKSTAKT--AVSSRSMMKFESGYTVETVFDGSKLGIDPYSVEMS 908
            GVVSNVVS+LVK LWS+KST KT  AVSSRSM+KFE GYTV+TVFDGSKLGI+P+SVE+S
Sbjct: 850  GVVSNVVSALVKWLWSLKSTTKTSSAVSSRSMIKFEGGYTVDTVFDGSKLGIEPHSVELS 909

Query: 909  PSGELLILDAENSNIYKISMPLSRYSRPKLVSGSAEGYPGHVDGHPREARMNHPKGLTLD 968
            P+GELL+LD ENSNIYKISMPLSRYSRPKL+SGS EGY GHVDG PR+ARMNHPKGLT+D
Sbjct: 910  PNGELLVLDFENSNIYKISMPLSRYSRPKLISGSPEGYSGHVDGRPRDARMNHPKGLTVD 969

Query: 969  ERGNIYIADTMNMAIRKISDTGVTTIAGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGSSC 1028
            +RGNIYIADTMNMAIRKI+D GVTTIAGG+W +G GH+DGPSEDAKFS+DFDV+YVGSSC
Sbjct: 970  DRGNIYIADTMNMAIRKITDAGVTTIAGGKWGRGGGHVDGPSEDAKFSSDFDVIYVGSSC 1029

Query: 1029 SLLVIDRGNKAIREIELNYDDCNTQYADSLNLGVVLLVAAGLFGYLLALLQRRVQAVFSS 1088
            SLLVIDRGN+AIREI+L+YDDCN  Y  +  LG+ +L+AA  FGY+LALLQRRVQA+FSS
Sbjct: 1030 SLLVIDRGNQAIREIQLHYDDCNDHYDGTFGLGIAMLIAAAFFGYMLALLQRRVQAMFSS 1089

Query: 1089 PKDEDVKSHQ-MKKAAQAAQYQRPPLKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSGS 1148
                 ++  + + +AA  A YQRPP KSVRP+LIP+EDEPEKL++GFFGSLG++ +N+G 
Sbjct: 1090 NDTSYLQDQRTIHRAATVAPYQRPP-KSVRPALIPNEDEPEKLDDGFFGSLGKIVLNTGL 1149

Query: 1149 SVADIVGGFLPGFRRKPLNNQIHQQFQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPTI 1208
            SV++I GG   GFR KP    I QQ+ Q N+H N+WP Q+S+V+PDEDEPPS+ET++ T 
Sbjct: 1150 SVSEIFGGIFTGFRSKPRQYHIQQQYHQANKHSNAWPTQDSYVVPDEDEPPSLETRSSTP 1209

Query: 1209 KKTYPFMTQDLDRSHQFKPNRNYFNGWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQIQ 1268
             KTYP+MT+DL++    K ++ Y+N W+  GE+ QQQQ   Q+  QQQQ+Q Q Q    Q
Sbjct: 1210 NKTYPYMTKDLEKPRHSKQSQAYYNSWE--GEYQQQQQLLTQL-QQQQQLQTQLQ----Q 1269

Query: 1269 QQQQQMQHHQQHHYQQYQQYHHRQYSAGPTTYYEKNCETKEIVFGAVQEQDGRREAMVIK 1302
            QQQ QMQ  QQH        HHR YS  PTTYYE++ ET EIVFGAVQEQDGRREA+VIK
Sbjct: 1270 QQQLQMQLQQQH--------HHRHYSTNPTTYYEESSETNEIVFGAVQEQDGRREAVVIK 1329

BLAST of CmaCh04G027160 vs. ExPASy TrEMBL
Match: A0A7J7CZX4 (Potassium transporter OS=Tripterygium wilfordii OX=458696 GN=HS088_TW12G00842 PE=3 SV=1)

HSP 1 Score: 1732.2 bits (4485), Expect = 0.0e+00
Identity = 889/1312 (67.76%), Postives = 1059/1312 (80.72%), Query Frame = 0

Query: 5    LIWGYPEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLC 64
            +I G P KRDSW+T+ TLAYQ+LGVVYGDLSTSPLYV+KSTFA+++QHSETNEE++GVL 
Sbjct: 6    VIQGNPIKRDSWKTVLTLAYQSLGVVYGDLSTSPLYVFKSTFAEDIQHSETNEEIFGVLS 65

Query: 65   FVFWTLTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKR 124
            F+FWTLTLIPL+KYVFIVL+ADDNGEGGTFALYSLLCRHAR+SSLPN QLADEELS YK+
Sbjct: 66   FIFWTLTLIPLVKYVFIVLRADDNGEGGTFALYSLLCRHARVSSLPNCQLADEELSEYKK 125

Query: 125  DTPEPTSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVE 184
            D     ++    S LK+T EKC+VL+++LLVLALIGTCMVIGDGVLTPAISVFSAVSG+E
Sbjct: 126  DEVVSNASRDIGSSLKSTFEKCRVLRKVLLVLALIGTCMVIGDGVLTPAISVFSAVSGLE 185

Query: 185  LSMAKEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYW 244
            LSM+KE HQY++VP+ACAILV LFA+QH+GTHRVGFLFAPVVI WLLC+SAIGLYNI +W
Sbjct: 186  LSMSKEQHQYVEVPVACAILVLLFAIQHFGTHRVGFLFAPVVITWLLCVSAIGLYNIVHW 245

Query: 245  NPLVYKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFV 304
            NP VY+ALSPYYMYKFLKKT+++GWM+LGG+LLCITGSEAMFADLGHFS  SIK+AFTFV
Sbjct: 246  NPQVYQALSPYYMYKFLKKTQRRGWMSLGGILLCITGSEAMFADLGHFSQLSIKIAFTFV 305

Query: 305  VYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITG 364
            VYPSL+LAYMGQAAYLSKHH +++DY+IGFYVSVPEK+RWP+LAIAILAAVVGSQA+ITG
Sbjct: 306  VYPSLILAYMGQAAYLSKHHIIESDYQIGFYVSVPEKIRWPILAIAILAAVVGSQAVITG 365

Query: 365  TFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAA 424
            TFS+IKQCS+L CFPRVKIVHTSSK+HGQIYIP INW LM+LCLAVT+GFR+T+ LGNAA
Sbjct: 366  TFSVIKQCSALSCFPRVKIVHTSSKMHGQIYIPEINWTLMLLCLAVTVGFRDTKHLGNAA 425

Query: 425  GLAVITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPI 484
            GLAVITVMLV+TCLMSLVIV CWHKS+ LA+ F+ F GSIEALYFSASL+KF EGAWVPI
Sbjct: 426  GLAVITVMLVTTCLMSLVIVFCWHKSIFLALCFIFFFGSIEALYFSASLIKFLEGAWVPI 485

Query: 485  ALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGI 544
            ALSL   +VM VWHYGT+KKYE DV+NKV +NW+L +GP +GIVRVRGIGL+HTELVSGI
Sbjct: 486  ALSLILFIVMCVWHYGTIKKYEFDVQNKVSVNWILSLGPSLGIVRVRGIGLLHTELVSGI 545

Query: 545  PSIFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRD 604
            P+IFSHFVTN+PAFH+++VFLC+KSV +PHVRPEERFLVGRVGP+EYRLYRCIARYGYRD
Sbjct: 546  PAIFSHFVTNLPAFHQVVVFLCVKSVPVPHVRPEERFLVGRVGPREYRLYRCIARYGYRD 605

Query: 605  VHKDDLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMC 664
            +HKDD+EFE+DL CSIAEFIRSE  ECN      ++D  D+ RM V+GTSS+   GI+MC
Sbjct: 606  IHKDDMEFEKDLVCSIAEFIRSEHPECNI----SVEDVGDDGRMTVVGTSSSNFEGIRMC 665

Query: 665  EN--ESCSLPIIDSSEIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIM 724
            E+  E       +  EI+S  + RKRVRF+VP+S ++D+  R EL+ELMEAREAG+AFI+
Sbjct: 666  EDDAEVSETGASELREIKSPEIGRKRVRFLVPQSPQVDIQAREELRELMEAREAGLAFIL 725

Query: 725  GHSYVKAKKGSEAKTGQLSFPLYSTLPLYVYLQSPKFLRVFP-----PQSSSFSTMARIS 784
            GHSYV+ K+GS      +    Y  L      + P +    P      Q+    TM R  
Sbjct: 726  GHSYVRTKRGSSLMKKIIINFGYDFLRR--NCRGPNYALSIPHASTLEQTRRNPTMCRNW 785

Query: 785  LAFALVFLLLFGGVSPASASPPGKIVRGVVSNVVSSLVKKLWSMKSTAKTAVSSRSMMKF 844
            +A  LVF+LL G  S  SA PP KIV G+VSNVV+ LVK LWS+KS  KT VSSRS+MKF
Sbjct: 786  VALGLVFVLLSGEFSSVSAIPPAKIVSGIVSNVVAGLVKWLWSLKSNTKTTVSSRSLMKF 845

Query: 845  ESGYTVETVFDGSKLGIDPYSVEMSPSGELLILDAENSNIYKISMPLSRYSRPKLVSGSA 904
            E GY V+TVFDGSKLGI PYS++MSP+G+LL+LD+ENSNIY+I MPLSRY+RPKLV+GS 
Sbjct: 846  EDGYIVDTVFDGSKLGIQPYSLDMSPNGDLLVLDSENSNIYRIPMPLSRYARPKLVAGSP 905

Query: 905  EGYPGHVDGHPREARMNHPKGLTLDERGNIYIADTMNMAIRKISDTGVTTIAGGRWNQGS 964
            EGY GHVDG  REAR+NHPKGLT+D+RGN+Y+ADT+NMAIRKISD+GVTTIAGG+W +G 
Sbjct: 906  EGYSGHVDGRLREARLNHPKGLTVDDRGNVYVADTLNMAIRKISDSGVTTIAGGKWTRGG 965

Query: 965  GHIDGPSEDAKFSNDFDVVYVGSSCSLLVIDRGNKAIREIELNYDDCNTQYADSLNLGVV 1024
            GH+DGPSEDAKFSNDFDV Y+ SSCSLLVIDRGN+AIREI+L  DDC+ QY  + +LG+ 
Sbjct: 966  GHVDGPSEDAKFSNDFDVFYIRSSCSLLVIDRGNQAIREIQLLSDDCSYQYDGNFHLGIS 1025

Query: 1025 LLVAAGLFGYLLALLQRRVQAVFSSPKDEDVKSHQMKKAAQAAQYQRPPLKSVRPSLIPS 1084
            +LVAAG FGY+LALLQ RV+A+FSS  D    S   KKA     YQR P  SVRP LIP 
Sbjct: 1026 VLVAAGFFGYILALLQYRVRAMFSSKND----SRTYKKAMPMPPYQRVP-NSVRPPLIPP 1085

Query: 1085 EDEPEKLEEGFFGSLGRLFVNSGSSVADIVGGFLPGFRRKPLNNQIHQQ-FQQVNRHPNS 1144
            + EPE  +EGFF S+GRLF+N+GSS A+I G     FR+K  N Q  QQ ++Q  +H N+
Sbjct: 1086 DIEPEISDEGFFTSIGRLFINTGSSAAEIFGTIFSVFRKKSPNYQYFQQPYEQQFKHSNT 1145

Query: 1145 WPLQESFVIPDEDEPPSIETKTPTIKKTYPFMTQDLDRSHQFKPNRNYFNGWDNGGEFHQ 1204
            W +QES+VIP EDEPPSIE+++PT KKTYPFM +DL++SH  K +   + GWD   E++Q
Sbjct: 1146 WSIQESYVIPHEDEPPSIESRSPTPKKTYPFMAKDLEKSHHLKQSHGSYGGWD--VEYYQ 1205

Query: 1205 QQQQPQQIHHQQQQIQQQPQQQQIQQQQQQMQHHQQHHYQQYQQYHHRQYSAGPTTYYEK 1264
            QQQ  +Q HH Q Q QQQ                            HR  ++ P T+YE+
Sbjct: 1206 QQQLQRQNHHPQHQQQQQ----------------------------HRHSASNPKTFYEQ 1265

Query: 1265 NCETKEIVFGAVQEQDGRREAMVIKAVDYGDPRYNHHNIRARYNYTGNPNSY 1309
            +CET EIVFGAVQEQ+GRREAMVIKAV+YGDP  NH NIR R NYTG  + Y
Sbjct: 1266 SCETNEIVFGAVQEQEGRREAMVIKAVNYGDPTNNHQNIRPRLNYTGRSHGY 1276

BLAST of CmaCh04G027160 vs. ExPASy TrEMBL
Match: A0A7J6H6S0 (Potassium transporter OS=Cannabis sativa OX=3483 GN=G4B88_015521 PE=3 SV=1)

HSP 1 Score: 1550.0 bits (4012), Expect = 0.0e+00
Identity = 822/1317 (62.41%), Postives = 978/1317 (74.26%), Query Frame = 0

Query: 11   EKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTL 70
            +K+DSW+T+ TLAYQ+LG+VYGDLSTSPLYVYKSTFA+++QHS+TNEE+YGVL FVFWTL
Sbjct: 13   KKKDSWKTVLTLAYQSLGIVYGDLSTSPLYVYKSTFAEDIQHSDTNEEIYGVLSFVFWTL 72

Query: 71   TLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYK-RDTPEP 130
            TLIPL+KYVFIVL+A+DNGEGGTFALYSLLCRHAR+SSLPN Q+AD++LS Y  +D    
Sbjct: 73   TLIPLVKYVFIVLRANDNGEGGTFALYSLLCRHARVSSLPNCQVADQDLSEYNTKDGVVS 132

Query: 131  TSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAK 190
             +  +    LK+TLEK +VLQ +LLVLALIGTCMVIGDGVLTPAIS              
Sbjct: 133  PTKTTAGLTLKSTLEKYRVLQTVLLVLALIGTCMVIGDGVLTPAIS-------------- 192

Query: 191  EHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVY 250
                 ++VP+ACA+L+FLFALQHYGTH+VGFLFAP+VI WL CISAIG+YNI +WNP VY
Sbjct: 193  ----DVEVPVACAVLIFLFALQHYGTHKVGFLFAPIVITWLFCISAIGVYNIIHWNPHVY 252

Query: 251  KALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSL 310
            +ALSPYYMYKFLKKT++ GWM+LGG+LLCITGSEAMFADLGHFS  SI++AFTFVVYPSL
Sbjct: 253  QALSPYYMYKFLKKTQRGGWMSLGGILLCITGSEAMFADLGHFSQLSIQIAFTFVVYPSL 312

Query: 311  VLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSII 370
            +LAYMGQAAYLS HH+L+ DYRIGFYVSVPEK+RWPVLAIAILAAVVGSQA+ITGTFSII
Sbjct: 313  ILAYMGQAAYLSMHHDLETDYRIGFYVSVPEKIRWPVLAIAILAAVVGSQAVITGTFSII 372

Query: 371  KQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVI 430
            KQC++LGCFP+VKIVHTSSK+HGQIYIP INW LM+LCL+VTIGFR+T+R+GNA+GLAVI
Sbjct: 373  KQCTALGCFPKVKIVHTSSKVHGQIYIPEINWTLMLLCLSVTIGFRDTKRMGNASGLAVI 432

Query: 431  TVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLT 490
            TVMLV+TCLMSLV+VLCWHKS+ LAI F+ F GSIEALYFSASL+KF EGAWVP+ALS  
Sbjct: 433  TVMLVTTCLMSLVMVLCWHKSIFLAICFIFFFGSIEALYFSASLIKFREGAWVPVALSFI 492

Query: 491  FLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFS 550
            FLVVMYVWHYGT KKYE+DV+NKV INWLLG+GP +GIVRVRGIGL+HTELVSGIP++FS
Sbjct: 493  FLVVMYVWHYGTQKKYESDVQNKVSINWLLGLGPNLGIVRVRGIGLIHTELVSGIPAVFS 552

Query: 551  HFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDD 610
            HFVTN+PAFH+++VFLC+KSV +PHVRPEERFLVGRVGPKEYRLYRCIARYGY D+HKDD
Sbjct: 553  HFVTNLPAFHQVVVFLCVKSVPVPHVRPEERFLVGRVGPKEYRLYRCIARYGYHDIHKDD 612

Query: 611  LEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCEN-ES 670
            LEFE+DL  SIAEFIRSE+ EC+      L++++ +E+M V+GT+S+ L GI++ E+   
Sbjct: 613  LEFEKDLVRSIAEFIRSERPECDM----RLEESESDEKMTVVGTASSNLKGIRLSEDGGD 672

Query: 671  CSLPIIDSS---EIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIMGHS 730
            CS     S+   EIRS +  RKRVRFV+PES ++D +T++ELQELMEAREAGMAFI+GHS
Sbjct: 673  CSERAGTSAELQEIRSPKKVRKRVRFVLPESPRIDRETQKELQELMEAREAGMAFILGHS 732

Query: 731  YVKAKKGS-------------------EAKTGQLSFPLYSTLPLYVYLQSPKFLRVFPPQ 790
            YV+AK GS                      +  LSFP  STL + +         V    
Sbjct: 733  YVRAKSGSGFMKRLVINIGYDFLRRNFRGPSYALSFPHASTLEVGMVYHKRWHNNVCGVS 792

Query: 791  SSSFSTMARISLAFALVFLLLFGGVSPASASPPGKIVRGVVSNVVSSLVKKLWSMKSTAK 850
              S +TMAR   A  L+ ++   G +  SA+PP KIV G+VSNVVS+LVK LWS+KST K
Sbjct: 793  YLSGTTMARSLTALLLILVIFSSGYASVSATPPAKIVSGIVSNVVSALVKWLWSLKSTPK 852

Query: 851  TAVSSRSMMKFESGYTVETVFDGSKLGIDPYSVEMSPSGELLILDAENSNIYKISMPLSR 910
            TA+SSRSMMKFESGYTVETVFDGSKLGI+PYSVE+SPSG+LL+LD+EN+           
Sbjct: 853  TAISSRSMMKFESGYTVETVFDGSKLGIEPYSVEVSPSGQLLVLDSENN----------- 912

Query: 911  YSRPKLVSGSAEGYPGHVDGHPREARMNHPKGLTLDERGNIYIADTMNMAIRKISDTGVT 970
             SRPKLV+GS EGY GHVDG PREAR+NHPKGLT D++GNIYIADTMNMAIRKISDTGVT
Sbjct: 913  -SRPKLVAGSPEGYAGHVDGKPREARLNHPKGLTADDKGNIYIADTMNMAIRKISDTGVT 972

Query: 971  TIAGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGSSCSLLVIDRGNKAIREIELNYDDCNT 1030
            TIAGG+W QG  H+DGPSEDAKFS+DFDV+Y+GSSCSLL+IDRGN+AIREI+LN DDC+ 
Sbjct: 973  TIAGGKWGQGGDHVDGPSEDAKFSDDFDVIYMGSSCSLLIIDRGNQAIREIQLNDDDCSY 1032

Query: 1031 QYADSLNLGVVLLVAAGLFGYLLALLQRRVQAVFSSPKDEDVKSHQMKKAAQAAQYQRPP 1090
            QY  S +LG+ +LVAA  FGY+LALLQRRV+A+FSS   EDV++  +K+    + YQ+PP
Sbjct: 1033 QYDSSFHLGIAMLVAAAFFGYMLALLQRRVRAMFSS--QEDVRT-PIKRTMPMSPYQKPP 1092

Query: 1091 LKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSGSSVADIVGGFLPGFRRKPLNNQIHQQ 1150
             KSVRP LIP+EDE E+ EEG FGS GRL +N+GSS+A+I GG   GFR KP + QI  Q
Sbjct: 1093 -KSVRPPLIPAEDEYERSEEGIFGSFGRLVLNTGSSMAEIFGGLFSGFRSKPRHYQIQHQ 1152

Query: 1151 FQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPTIKKTYPFMTQDLDRSHQFKPNRNYFN 1210
            F Q +++ NSWP+QESF                                           
Sbjct: 1153 FHQQSKYSNSWPMQESF------------------------------------------- 1163

Query: 1211 GWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQIQQQQQQMQHHQQHHYQQYQQYHHRQY 1270
                                                                        
Sbjct: 1213 ------------------------------------------------------------ 1163

Query: 1271 SAGPTTYYEKNCETKEIVFGAVQEQDGRREAMVIKAVDYGDPRYNHHNIRARYNYTG 1304
                                     DGRREA+VIKAVDYGDP YNHHNIR R NYTG
Sbjct: 1273 -------------------------DGRREAVVIKAVDYGDPVYNHHNIRPRLNYTG 1163

BLAST of CmaCh04G027160 vs. ExPASy TrEMBL
Match: A0A565CLI0 (Potassium transporter OS=Arabis nemorensis OX=586526 GN=ANE_LOCUS24969 PE=3 SV=1)

HSP 1 Score: 1471.4 bits (3808), Expect = 0.0e+00
Identity = 806/1321 (61.01%), Postives = 980/1321 (74.19%), Query Frame = 0

Query: 12   KRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTLT 71
            K++SW T+ TLAYQ+LGVVYGDL+TSPLYVYKSTFA+++QHSETNEE++GVL  VFWTLT
Sbjct: 14   KKESWWTVLTLAYQSLGVVYGDLATSPLYVYKSTFAEDIQHSETNEEIFGVLSLVFWTLT 73

Query: 72   LIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEPTS 131
            LIPL+KYVFIVL+ADDNGEGGTFALYSLLCRHAR+SSLPN QLADE+LS YK+D      
Sbjct: 74   LIPLVKYVFIVLRADDNGEGGTFALYSLLCRHARISSLPNFQLADEDLSEYKKDYGANPR 133

Query: 132  NFSFSS-CLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAKE 191
            N   +   LK  LEK KVLQ +LLVLALIGTCMVIGDGVLTPAISVFSAVSG+ELSM+KE
Sbjct: 134  NLKVTGWSLKGVLEKYKVLQNMLLVLALIGTCMVIGDGVLTPAISVFSAVSGLELSMSKE 193

Query: 192  HHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVYK 251
             HQY++VP+ CAIL+ LF+LQHYGTHR+GFLFAP+V+ WLLCIS IG+YNIF+WNP VYK
Sbjct: 194  QHQYVEVPVVCAILILLFSLQHYGTHRLGFLFAPIVLAWLLCISTIGVYNIFHWNPHVYK 253

Query: 252  ALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSLV 311
            ALSPYY+YKFLKKT+K+GWM+LGG+LLCITGSEAMFADLGHF+  SI++AFTF VYPSL+
Sbjct: 254  ALSPYYIYKFLKKTRKRGWMSLGGILLCITGSEAMFADLGHFTQLSIQIAFTFAVYPSLI 313

Query: 312  LAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSIIK 371
            LAYMGQAAYLSKHH L+ DYR+GFYVSVPE++RWPVLAIAILAAVVGSQAIITGTFSIIK
Sbjct: 314  LAYMGQAAYLSKHHFLETDYRVGFYVSVPERIRWPVLAIAILAAVVGSQAIITGTFSIIK 373

Query: 372  QCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVIT 431
            QC+SLGCFP+VKI+HTSSK+HGQIYIP INW LM+LCLAVT+GFR+T+ + NA+GLAVIT
Sbjct: 374  QCTSLGCFPKVKIMHTSSKMHGQIYIPEINWTLMLLCLAVTVGFRDTKHISNASGLAVIT 433

Query: 432  VMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLTF 491
            VMLV+TCLMSLVIVLCWHK+ + A+AF+ F G+IEALYFSASL+KF EGAWVP+ALS  F
Sbjct: 434  VMLVTTCLMSLVIVLCWHKNSIFALAFIFFFGTIEALYFSASLIKFLEGAWVPLALSFIF 493

Query: 492  LVVMYVWHYGTLKKYEADVENKVPINWLLGI--GPKIGIVRVRGIGLVHTELVSGIPSIF 551
            L++MYVWHYGTLK+YE DV+NKV INWLL +     +GIVRVRGIG+++TELVSGIP+IF
Sbjct: 494  LLIMYVWHYGTLKRYEFDVQNKVSINWLLTLFGSSNLGIVRVRGIGVINTELVSGIPAIF 553

Query: 552  SHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKD 611
            SHFVTN+PAFH+I+VFLC+KSV +PHV+PEERFLVGRVGPKE+RLYRCIARYGYRDVHKD
Sbjct: 554  SHFVTNLPAFHQIVVFLCVKSVPVPHVKPEERFLVGRVGPKEHRLYRCIARYGYRDVHKD 613

Query: 612  DLEFERDLACSIAEFIRSEKT-ECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMC--- 671
            DLEFE DL CSIAEFIRS+K  +C   + N+      NER+ V+  SS+ L G+Q+    
Sbjct: 614  DLEFEHDLICSIAEFIRSDKPFDCLADQENEC---GTNERLTVVAASSSNLEGVQIYADD 673

Query: 672  ------ENESCSLPIIDSSE--IRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREA 731
                  E  S S  I+ + E  + S  + +KRVRFV+PES ++D  T  EL ELMEAREA
Sbjct: 674  GGFNKPEPSSSSEVIVVAVEPQVPSPGI-KKRVRFVLPESARIDRSTEEELNELMEAREA 733

Query: 732  GMAFIMGHSYVKAKKGSEA-KTGQLSFPLYSTLPLYVYLQSPKFLRVFPPQSSSFSTMAR 791
            GMAFIMGHSYV+AK GS   K   ++F        Y +L+       +   +   ST+  
Sbjct: 734  GMAFIMGHSYVRAKSGSSVMKKIAINFG-------YDFLRRNSRGPCYGLSTPHASTLEV 793

Query: 792  ISLAFALVFLLLFGGVSPASASPPGKIVRGVVSNVVSSLVKKLWSMK------STAKTAV 851
            +S                 +  PP KIV G+V+NV S L K LWS++      +T K+ V
Sbjct: 794  VS-----------------AKPPPVKIVSGLVTNVASLLWKWLWSLQTSTTTTTTTKSGV 853

Query: 852  SSRSMMKFESGYTVETVFDGSKLGIDPYSVEMSPS-GELLILDAENSNIYKISMPLSRYS 911
            SSRSM+K+ESGY +ETVFDGSKLGI+PY++++SP+ GEL++LD+ENSNI+KISMPLSRY 
Sbjct: 854  SSRSMVKYESGYNIETVFDGSKLGIEPYAIDVSPNGGELIVLDSENSNIHKISMPLSRYG 913

Query: 912  RPKLVSGSAEGYPGHVDGHPREARMNHPKGLTLDERGNIYIADTMNMAIRKISDTGVTTI 971
            +PKLVSGS EGY GHVDG  +EA+MN P+GL +D+ GNIY+ADT NMAIRKISD GV+TI
Sbjct: 914  KPKLVSGSQEGYTGHVDGKLKEAKMNRPRGLAIDDSGNIYVADTNNMAIRKISDDGVSTI 973

Query: 972  -AGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGSSCSLLVIDRGNKAIREIELNYDDCNTQ 1031
             AGGRW+ GS       E  +FS+DFD++YV SSCSLLVIDRGN+ I+EI+L+  DC+  
Sbjct: 974  AAGGRWSSGS-----KEESMRFSDDFDLIYVSSSCSLLVIDRGNQMIKEIQLHDHDCSHH 1033

Query: 1032 Y----ADSLNLGVVLLVAAGLFGYLLALLQRRVQAVFSSPKDEDVKSHQMKKAAQAAQYQ 1091
                  D   LG  LLVAAG FGY+LALL RRV+++FSS +  D K H  K+    A YQ
Sbjct: 1034 EPEPDTDLQYLGTALLVAAGFFGYMLALLVRRVRSLFSSSR-HDNKRHVAKQNMTMAPYQ 1093

Query: 1092 RPPLKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSGSSVADIVGGFLPGFRRKPLNNQI 1151
            R P + VR  LIP + EPEK EEGF GSLG+L V +GSSV++++ G   G    P N Q 
Sbjct: 1094 RYP-RPVRQPLIPPQHEPEK-EEGFLGSLGKLVVKTGSSVSEMMTGSRNGI---PQNYQY 1153

Query: 1152 HQQFQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPTIKKTYPFMTQDLDRSHQFKPNRN 1211
            H Q     + PN WP+QESF IP+ED PP++E ++  I    P++     R      NR+
Sbjct: 1154 HHQ-----QEPNQWPVQESFAIPEEDGPPALEPRSGIISDK-PYL-----RPQGTNQNRS 1213

Query: 1212 YFNGWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQIQQQQQQMQHHQQHHYQQYQQYHH 1271
            Y                                                  YQ Y QY +
Sbjct: 1214 Y--------------------------------------------------YQDYDQYQN 1234

Query: 1272 RQ-YSAGPTTYYEKNCETKEIVFGAVQEQDGRREAMVIKAVDYGDPRYNHHNIRARYNYT 1304
            +Q  +   T  +E+N E  EIVFGAVQEQDGRREAMVIKAVD+ +   +  N+R R NY 
Sbjct: 1274 QQKRNVNDTASFEENREKNEIVFGAVQEQDGRREAMVIKAVDFNESMNDQRNLRPRINYM 1234

BLAST of CmaCh04G027160 vs. ExPASy TrEMBL
Match: A0A6J1JNB7 (Potassium transporter OS=Cucurbita maxima OX=3661 GN=LOC111487413 PE=3 SV=1)

HSP 1 Score: 1449.9 bits (3752), Expect = 0.0e+00
Identity = 733/733 (100.00%), Postives = 733/733 (100.00%), Query Frame = 0

Query: 1   MDPNLIWGYPEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVY 60
           MDPNLIWGYPEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVY
Sbjct: 1   MDPNLIWGYPEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVY 60

Query: 61  GVLCFVFWTLTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELS 120
           GVLCFVFWTLTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELS
Sbjct: 61  GVLCFVFWTLTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELS 120

Query: 121 AYKRDTPEPTSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAV 180
           AYKRDTPEPTSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAV
Sbjct: 121 AYKRDTPEPTSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAV 180

Query: 181 SGVELSMAKEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYN 240
           SGVELSMAKEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYN
Sbjct: 181 SGVELSMAKEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYN 240

Query: 241 IFYWNPLVYKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLA 300
           IFYWNPLVYKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLA
Sbjct: 241 IFYWNPLVYKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLA 300

Query: 301 FTFVVYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQA 360
           FTFVVYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQA
Sbjct: 301 FTFVVYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQA 360

Query: 361 IITGTFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERL 420
           IITGTFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERL
Sbjct: 361 IITGTFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERL 420

Query: 421 GNAAGLAVITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGA 480
           GNAAGLAVITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGA
Sbjct: 421 GNAAGLAVITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGA 480

Query: 481 WVPIALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTEL 540
           WVPIALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTEL
Sbjct: 481 WVPIALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTEL 540

Query: 541 VSGIPSIFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARY 600
           VSGIPSIFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARY
Sbjct: 541 VSGIPSIFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARY 600

Query: 601 GYRDVHKDDLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNG 660
           GYRDVHKDDLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNG
Sbjct: 601 GYRDVHKDDLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNG 660

Query: 661 IQMCENESCSLPIIDSSEIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAF 720
           IQMCENESCSLPIIDSSEIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAF
Sbjct: 661 IQMCENESCSLPIIDSSEIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAF 720

Query: 721 IMGHSYVKAKKGS 734
           IMGHSYVKAKKGS
Sbjct: 721 IMGHSYVKAKKGS 733

BLAST of CmaCh04G027160 vs. NCBI nr
Match: KAG6602446.1 (Potassium transporter 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1911.0 bits (4949), Expect = 0.0e+00
Identity = 993/1048 (94.75%), Postives = 1002/1048 (95.61%), Query Frame = 0

Query: 297  IKLAFTFVVYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVV 356
            ++LAFTFVVYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVV
Sbjct: 2    LQLAFTFVVYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVV 61

Query: 357  GSQAIITGTFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRE 416
            GSQAIITGTFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRE
Sbjct: 62   GSQAIITGTFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRE 121

Query: 417  TERLGNAAGLAVITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKF 476
            TERLGNAAGLAVITVMLVSTCLMSLVIVLCWHK+V LAIAFLVF GSIE LYFSASLMKF
Sbjct: 122  TERLGNAAGLAVITVMLVSTCLMSLVIVLCWHKTVFLAIAFLVFFGSIETLYFSASLMKF 181

Query: 477  FEGAWVPIALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLV 536
            FEGAWVPIALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLV
Sbjct: 182  FEGAWVPIALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLV 241

Query: 537  HTELVSGIPSIFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRC 596
            HTELVSGIPSIFSHFVTN+PAFH+ILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRC
Sbjct: 242  HTELVSGIPSIFSHFVTNVPAFHQILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRC 301

Query: 597  IARYGYRDVHKDDLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSST 656
            IARYGYRDVHKDDLEFERDLACSIAEFIRSEKTECNT RH DLDDNDDNERMRVIGTSST
Sbjct: 302  IARYGYRDVHKDDLEFERDLACSIAEFIRSEKTECNTSRHEDLDDNDDNERMRVIGTSST 361

Query: 657  QLNGIQMCENESCSLPIIDSSEIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREA 716
            QLNGI+MCENESCSLPI  SSEIRSERLR+KRVRFV+PESTKMDVDTRRELQELMEAREA
Sbjct: 362  QLNGIRMCENESCSLPITSSSEIRSERLRKKRVRFVLPESTKMDVDTRRELQELMEAREA 421

Query: 717  GMAFIMGHSYVKAKKGS------------------------------------EAKTGQL 776
            GMAFIMGHSYVKAKKGS                                    EAKTGQL
Sbjct: 422  GMAFIMGHSYVKAKKGSGWMKKVVLNYGYDFLRKNSRGPSYALSIPNASTLEVEAKTGQL 481

Query: 777  SFPLYSTLPLYVYLQSPKFLRVFPPQSSSFSTMARISLAFALVFLLLFGGVSPASASPPG 836
            SFPLY TLPLYVYLQSPKFLRVFPPQSSSFSTMARISLAFALVFLLLFGGVSPASASPPG
Sbjct: 482  SFPLYCTLPLYVYLQSPKFLRVFPPQSSSFSTMARISLAFALVFLLLFGGVSPASASPPG 541

Query: 837  KIVRGVVSNVVSSLVKKLWSMKSTAKTAVSSRSMMKFESGYTVETVFDGSKLGIDPYSVE 896
            KIVRGVVSNVVSSLVKKLWSMKSTAKTAVSSRSMMKFESGYTVETVFDGSKLGIDPYSVE
Sbjct: 542  KIVRGVVSNVVSSLVKKLWSMKSTAKTAVSSRSMMKFESGYTVETVFDGSKLGIDPYSVE 601

Query: 897  MSPSGELLILDAENSNIYKISMPLSRYSRPKLVSGSAEGYPGHVDGHPREARMNHPKGLT 956
            MSPSGELLILDAENSNIYKISMPLSRYSRPKLVSGSAEGYPGHVDGHPREARMNHPKGLT
Sbjct: 602  MSPSGELLILDAENSNIYKISMPLSRYSRPKLVSGSAEGYPGHVDGHPREARMNHPKGLT 661

Query: 957  LDERGNIYIADTMNMAIRKISDTGVTTIAGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGS 1016
            LDERGNIYIADTMNMAIRKISDTGVTTIAGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGS
Sbjct: 662  LDERGNIYIADTMNMAIRKISDTGVTTIAGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGS 721

Query: 1017 SCSLLVIDRGNKAIREIELNYDDCNTQYADSLNLGVVLLVAAGLFGYLLALLQRRVQAVF 1076
            SCSLLVIDRGNKAIREIELNYDDCNTQYADSLNLGVVLLVAAGLFGYLLALLQRRVQAV 
Sbjct: 722  SCSLLVIDRGNKAIREIELNYDDCNTQYADSLNLGVVLLVAAGLFGYLLALLQRRVQAVL 781

Query: 1077 SSPKDEDVKSHQMKKAAQAAQYQRPPLKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSG 1136
            SSPKDEDV+SHQMKKAAQAAQYQRPPLKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSG
Sbjct: 782  SSPKDEDVRSHQMKKAAQAAQYQRPPLKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSG 841

Query: 1137 SSVADIVGGFLPGFRRKPLNNQIHQQFQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPT 1196
            SSVADIVGGFLPGFRRKPLNNQIHQQFQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPT
Sbjct: 842  SSVADIVGGFLPGFRRKPLNNQIHQQFQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPT 901

Query: 1197 IKKTYPFMTQDLDRSHQFKPNRNYFNGWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQI 1256
            IKKTYPFMTQDLDRSHQFKPNRNYFNGWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQI
Sbjct: 902  IKKTYPFMTQDLDRSHQFKPNRNYFNGWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQI 961

Query: 1257 QQQQQQMQHHQQHHYQQYQQYHHRQYSAGPTTYYEKNCETKEIVFGAVQEQDGRREAMVI 1309
             QQQQQMQHHQQHHYQQYQQYHHRQYSAGPTTYYEKNCETKEIVFGAVQEQDGRREAMVI
Sbjct: 962  -QQQQQMQHHQQHHYQQYQQYHHRQYSAGPTTYYEKNCETKEIVFGAVQEQDGRREAMVI 1021

BLAST of CmaCh04G027160 vs. NCBI nr
Match: RXH89440.1 (hypothetical protein DVH24_031797 [Malus domestica])

HSP 1 Score: 1777.7 bits (4603), Expect = 0.0e+00
Identity = 935/1340 (69.78%), Postives = 1101/1340 (82.16%), Query Frame = 0

Query: 9    YPEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFW 68
            +P K+DSW+T+ TLAYQ+LGVVYGDLSTSPLYVYKSTFA+++ HSETNEE++GVL FVFW
Sbjct: 10   HPIKKDSWKTLLTLAYQSLGVVYGDLSTSPLYVYKSTFAEDIHHSETNEEIFGVLSFVFW 69

Query: 69   TLTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPE 128
            TLTLIPL+KYVFIVL+ADDNGEGGTFALYSLL RHAR+SSLPN QLADEELS Y +D   
Sbjct: 70   TLTLIPLVKYVFIVLRADDNGEGGTFALYSLLSRHARVSSLPNCQLADEELSEYTKDGVV 129

Query: 129  PTSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMA 188
            PT+N +F S LK+TLEK KVL+++LLVLALIGTCMVIGDGVLTPAISVFSAVSG+ELSM+
Sbjct: 130  PTANSAFGSSLKSTLEKHKVLKKVLLVLALIGTCMVIGDGVLTPAISVFSAVSGLELSMS 189

Query: 189  KEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLV 248
            K+ H+Y++VP+AC IL+FLFALQHYGTHRVGFLFAP+VI WLLCIS+IG+YNIF+WNP V
Sbjct: 190  KQQHRYVEVPVACVILIFLFALQHYGTHRVGFLFAPIVITWLLCISSIGVYNIFHWNPHV 249

Query: 249  YKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPS 308
            Y+ALSPYYMYKFLKKT+K GWM+LGG+LLC+TGSEAMFADLGHFS  SIK+AFTFVVYPS
Sbjct: 250  YQALSPYYMYKFLKKTRKGGWMSLGGILLCMTGSEAMFADLGHFSQTSIKIAFTFVVYPS 309

Query: 309  LVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSI 368
            L+LAYMGQAAYLS+HH + +DYRIGFY SVPEK+RWPVLAIAILAAVVGSQAIITGTFSI
Sbjct: 310  LILAYMGQAAYLSRHHIVASDYRIGFYESVPEKIRWPVLAIAILAAVVGSQAIITGTFSI 369

Query: 369  IKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAV 428
            IKQCS+LGCFPRVKI+HTSSKIHGQIYIP INW LM+LCLAVTIGFR+T+ +GNA+GLAV
Sbjct: 370  IKQCSALGCFPRVKIIHTSSKIHGQIYIPEINWTLMLLCLAVTIGFRDTKSMGNASGLAV 429

Query: 429  ITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSL 488
            ITVMLV+TCLMSLVIVLCWHKS+  A+ F++F GS+EALYFSASL+KF EGAWVP+ALS 
Sbjct: 430  ITVMLVTTCLMSLVIVLCWHKSLFWAVCFILFFGSLEALYFSASLIKFREGAWVPVALSF 489

Query: 489  TFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIF 548
             FL+VMYVW YGT+KKYE DV+NKV INWLL +GP +GIVRVRGIGL+HTELVSGIP+IF
Sbjct: 490  IFLMVMYVWQYGTIKKYEFDVQNKVSINWLLSLGPTLGIVRVRGIGLIHTELVSGIPAIF 549

Query: 549  SHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKD 608
            SHFVTN+PAFH+++VFLCIKSV +PHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKD
Sbjct: 550  SHFVTNLPAFHQVVVFLCIKSVPVPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKD 609

Query: 609  DLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCENES 668
            D+EFERDL CSIAEFIRSE+ ECN       +  +D+E+M V+GTSS+ L+GI+M  +E+
Sbjct: 610  DMEFERDLVCSIAEFIRSERPECNL----SPEQLEDDEKMTVVGTSSSNLDGIRMSVDEA 669

Query: 669  CSLPIIDSS---EIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIMGHS 728
                I  +S   EIR     +KRVRFVVPE+ ++D +   ELQELMEAREAGMAFI+GHS
Sbjct: 670  DFSEIASTSELQEIRPTERAKKRVRFVVPETPRIDREAVEELQELMEAREAGMAFILGHS 729

Query: 729  YVKAKKGS-------------------EAKTGQLSFPLYSTLPL-YVY-----LQSP--- 788
            YVKAK+GS                      +  LS P  STL +  VY     +QS    
Sbjct: 730  YVKAKRGSSFMKKIVINFGYDFLRRNFRGPSYALSIPHASTLEVGMVYHSRWAMQSASDE 789

Query: 789  ------------KFLRVFPPQSSSFSTMARISLAFALVFLLLFGGVSP-ASASPPGKIVR 848
                        K++        S ++M R  +A  LVFLLL G  S  ASA+PP KIV 
Sbjct: 790  KVTEHCLWSLLLKWISYKLRPFKSSASMVRNWVALLLVFLLLSGDFSSGASATPPAKIVT 849

Query: 849  GVVSNVVSSLVKKLWSMKSTAKT--AVSSRSMMKFESGYTVETVFDGSKLGIDPYSVEMS 908
            GVVSNVVS+LVK LWS+KST KT  AVSSRSM+KFE GYTV+TVFDGSKLGI+P+SVE+S
Sbjct: 850  GVVSNVVSALVKWLWSLKSTTKTSSAVSSRSMIKFEGGYTVDTVFDGSKLGIEPHSVELS 909

Query: 909  PSGELLILDAENSNIYKISMPLSRYSRPKLVSGSAEGYPGHVDGHPREARMNHPKGLTLD 968
            P+GELL+LD ENSNIYKISMPLSRYSRPKL+SGS EGY GHVDG PR+ARMNHPKGLT+D
Sbjct: 910  PNGELLVLDFENSNIYKISMPLSRYSRPKLISGSPEGYSGHVDGRPRDARMNHPKGLTVD 969

Query: 969  ERGNIYIADTMNMAIRKISDTGVTTIAGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGSSC 1028
            +RGNIYIADTMNMAIRKI+D GVTTIAGG+W +G GH+DGPSEDAKFS+DFDV+YVGSSC
Sbjct: 970  DRGNIYIADTMNMAIRKITDAGVTTIAGGKWGRGGGHVDGPSEDAKFSSDFDVIYVGSSC 1029

Query: 1029 SLLVIDRGNKAIREIELNYDDCNTQYADSLNLGVVLLVAAGLFGYLLALLQRRVQAVFSS 1088
            SLLVIDRGN+AIREI+L+YDDCN  Y  +  LG+ +L+AA  FGY+LALLQRRVQA+FSS
Sbjct: 1030 SLLVIDRGNQAIREIQLHYDDCNDHYDGTFGLGIAMLIAAAFFGYMLALLQRRVQAMFSS 1089

Query: 1089 PKDEDVKSHQ-MKKAAQAAQYQRPPLKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSGS 1148
                 ++  + + +AA  A YQRPP KSVRP+LIP+EDEPEKL++GFFGSLG++ +N+G 
Sbjct: 1090 NDTSYLQDQRTIHRAATVAPYQRPP-KSVRPALIPNEDEPEKLDDGFFGSLGKIVLNTGL 1149

Query: 1149 SVADIVGGFLPGFRRKPLNNQIHQQFQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPTI 1208
            SV++I GG   GFR KP    I QQ+ Q N+H N+WP Q+S+V+PDEDEPPS+ET++ T 
Sbjct: 1150 SVSEIFGGIFTGFRSKPRQYHIQQQYHQANKHSNAWPTQDSYVVPDEDEPPSLETRSSTP 1209

Query: 1209 KKTYPFMTQDLDRSHQFKPNRNYFNGWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQIQ 1268
             KTYP+MT+DL++    K ++ Y+N W+  GE+ QQQQ   Q+  QQQQ+Q Q Q    Q
Sbjct: 1210 NKTYPYMTKDLEKPRHSKQSQAYYNSWE--GEYQQQQQLLTQL-QQQQQLQTQLQ----Q 1269

Query: 1269 QQQQQMQHHQQHHYQQYQQYHHRQYSAGPTTYYEKNCETKEIVFGAVQEQDGRREAMVIK 1302
            QQQ QMQ  QQH        HHR YS  PTTYYE++ ET EIVFGAVQEQDGRREA+VIK
Sbjct: 1270 QQQLQMQLQQQH--------HHRHYSTNPTTYYEESSETNEIVFGAVQEQDGRREAVVIK 1329

BLAST of CmaCh04G027160 vs. NCBI nr
Match: KAF5739633.1 (potassium transporter 8-like [Tripterygium wilfordii])

HSP 1 Score: 1732.2 bits (4485), Expect = 0.0e+00
Identity = 889/1312 (67.76%), Postives = 1059/1312 (80.72%), Query Frame = 0

Query: 5    LIWGYPEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLC 64
            +I G P KRDSW+T+ TLAYQ+LGVVYGDLSTSPLYV+KSTFA+++QHSETNEE++GVL 
Sbjct: 6    VIQGNPIKRDSWKTVLTLAYQSLGVVYGDLSTSPLYVFKSTFAEDIQHSETNEEIFGVLS 65

Query: 65   FVFWTLTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKR 124
            F+FWTLTLIPL+KYVFIVL+ADDNGEGGTFALYSLLCRHAR+SSLPN QLADEELS YK+
Sbjct: 66   FIFWTLTLIPLVKYVFIVLRADDNGEGGTFALYSLLCRHARVSSLPNCQLADEELSEYKK 125

Query: 125  DTPEPTSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVE 184
            D     ++    S LK+T EKC+VL+++LLVLALIGTCMVIGDGVLTPAISVFSAVSG+E
Sbjct: 126  DEVVSNASRDIGSSLKSTFEKCRVLRKVLLVLALIGTCMVIGDGVLTPAISVFSAVSGLE 185

Query: 185  LSMAKEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYW 244
            LSM+KE HQY++VP+ACAILV LFA+QH+GTHRVGFLFAPVVI WLLC+SAIGLYNI +W
Sbjct: 186  LSMSKEQHQYVEVPVACAILVLLFAIQHFGTHRVGFLFAPVVITWLLCVSAIGLYNIVHW 245

Query: 245  NPLVYKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFV 304
            NP VY+ALSPYYMYKFLKKT+++GWM+LGG+LLCITGSEAMFADLGHFS  SIK+AFTFV
Sbjct: 246  NPQVYQALSPYYMYKFLKKTQRRGWMSLGGILLCITGSEAMFADLGHFSQLSIKIAFTFV 305

Query: 305  VYPSLVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITG 364
            VYPSL+LAYMGQAAYLSKHH +++DY+IGFYVSVPEK+RWP+LAIAILAAVVGSQA+ITG
Sbjct: 306  VYPSLILAYMGQAAYLSKHHIIESDYQIGFYVSVPEKIRWPILAIAILAAVVGSQAVITG 365

Query: 365  TFSIIKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAA 424
            TFS+IKQCS+L CFPRVKIVHTSSK+HGQIYIP INW LM+LCLAVT+GFR+T+ LGNAA
Sbjct: 366  TFSVIKQCSALSCFPRVKIVHTSSKMHGQIYIPEINWTLMLLCLAVTVGFRDTKHLGNAA 425

Query: 425  GLAVITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPI 484
            GLAVITVMLV+TCLMSLVIV CWHKS+ LA+ F+ F GSIEALYFSASL+KF EGAWVPI
Sbjct: 426  GLAVITVMLVTTCLMSLVIVFCWHKSIFLALCFIFFFGSIEALYFSASLIKFLEGAWVPI 485

Query: 485  ALSLTFLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGI 544
            ALSL   +VM VWHYGT+KKYE DV+NKV +NW+L +GP +GIVRVRGIGL+HTELVSGI
Sbjct: 486  ALSLILFIVMCVWHYGTIKKYEFDVQNKVSVNWILSLGPSLGIVRVRGIGLLHTELVSGI 545

Query: 545  PSIFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRD 604
            P+IFSHFVTN+PAFH+++VFLC+KSV +PHVRPEERFLVGRVGP+EYRLYRCIARYGYRD
Sbjct: 546  PAIFSHFVTNLPAFHQVVVFLCVKSVPVPHVRPEERFLVGRVGPREYRLYRCIARYGYRD 605

Query: 605  VHKDDLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMC 664
            +HKDD+EFE+DL CSIAEFIRSE  ECN      ++D  D+ RM V+GTSS+   GI+MC
Sbjct: 606  IHKDDMEFEKDLVCSIAEFIRSEHPECNI----SVEDVGDDGRMTVVGTSSSNFEGIRMC 665

Query: 665  EN--ESCSLPIIDSSEIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIM 724
            E+  E       +  EI+S  + RKRVRF+VP+S ++D+  R EL+ELMEAREAG+AFI+
Sbjct: 666  EDDAEVSETGASELREIKSPEIGRKRVRFLVPQSPQVDIQAREELRELMEAREAGLAFIL 725

Query: 725  GHSYVKAKKGSEAKTGQLSFPLYSTLPLYVYLQSPKFLRVFP-----PQSSSFSTMARIS 784
            GHSYV+ K+GS      +    Y  L      + P +    P      Q+    TM R  
Sbjct: 726  GHSYVRTKRGSSLMKKIIINFGYDFLRR--NCRGPNYALSIPHASTLEQTRRNPTMCRNW 785

Query: 785  LAFALVFLLLFGGVSPASASPPGKIVRGVVSNVVSSLVKKLWSMKSTAKTAVSSRSMMKF 844
            +A  LVF+LL G  S  SA PP KIV G+VSNVV+ LVK LWS+KS  KT VSSRS+MKF
Sbjct: 786  VALGLVFVLLSGEFSSVSAIPPAKIVSGIVSNVVAGLVKWLWSLKSNTKTTVSSRSLMKF 845

Query: 845  ESGYTVETVFDGSKLGIDPYSVEMSPSGELLILDAENSNIYKISMPLSRYSRPKLVSGSA 904
            E GY V+TVFDGSKLGI PYS++MSP+G+LL+LD+ENSNIY+I MPLSRY+RPKLV+GS 
Sbjct: 846  EDGYIVDTVFDGSKLGIQPYSLDMSPNGDLLVLDSENSNIYRIPMPLSRYARPKLVAGSP 905

Query: 905  EGYPGHVDGHPREARMNHPKGLTLDERGNIYIADTMNMAIRKISDTGVTTIAGGRWNQGS 964
            EGY GHVDG  REAR+NHPKGLT+D+RGN+Y+ADT+NMAIRKISD+GVTTIAGG+W +G 
Sbjct: 906  EGYSGHVDGRLREARLNHPKGLTVDDRGNVYVADTLNMAIRKISDSGVTTIAGGKWTRGG 965

Query: 965  GHIDGPSEDAKFSNDFDVVYVGSSCSLLVIDRGNKAIREIELNYDDCNTQYADSLNLGVV 1024
            GH+DGPSEDAKFSNDFDV Y+ SSCSLLVIDRGN+AIREI+L  DDC+ QY  + +LG+ 
Sbjct: 966  GHVDGPSEDAKFSNDFDVFYIRSSCSLLVIDRGNQAIREIQLLSDDCSYQYDGNFHLGIS 1025

Query: 1025 LLVAAGLFGYLLALLQRRVQAVFSSPKDEDVKSHQMKKAAQAAQYQRPPLKSVRPSLIPS 1084
            +LVAAG FGY+LALLQ RV+A+FSS  D    S   KKA     YQR P  SVRP LIP 
Sbjct: 1026 VLVAAGFFGYILALLQYRVRAMFSSKND----SRTYKKAMPMPPYQRVP-NSVRPPLIPP 1085

Query: 1085 EDEPEKLEEGFFGSLGRLFVNSGSSVADIVGGFLPGFRRKPLNNQIHQQ-FQQVNRHPNS 1144
            + EPE  +EGFF S+GRLF+N+GSS A+I G     FR+K  N Q  QQ ++Q  +H N+
Sbjct: 1086 DIEPEISDEGFFTSIGRLFINTGSSAAEIFGTIFSVFRKKSPNYQYFQQPYEQQFKHSNT 1145

Query: 1145 WPLQESFVIPDEDEPPSIETKTPTIKKTYPFMTQDLDRSHQFKPNRNYFNGWDNGGEFHQ 1204
            W +QES+VIP EDEPPSIE+++PT KKTYPFM +DL++SH  K +   + GWD   E++Q
Sbjct: 1146 WSIQESYVIPHEDEPPSIESRSPTPKKTYPFMAKDLEKSHHLKQSHGSYGGWD--VEYYQ 1205

Query: 1205 QQQQPQQIHHQQQQIQQQPQQQQIQQQQQQMQHHQQHHYQQYQQYHHRQYSAGPTTYYEK 1264
            QQQ  +Q HH Q Q QQQ                            HR  ++ P T+YE+
Sbjct: 1206 QQQLQRQNHHPQHQQQQQ----------------------------HRHSASNPKTFYEQ 1265

Query: 1265 NCETKEIVFGAVQEQDGRREAMVIKAVDYGDPRYNHHNIRARYNYTGNPNSY 1309
            +CET EIVFGAVQEQ+GRREAMVIKAV+YGDP  NH NIR R NYTG  + Y
Sbjct: 1266 SCETNEIVFGAVQEQEGRREAMVIKAVNYGDPTNNHQNIRPRLNYTGRSHGY 1276

BLAST of CmaCh04G027160 vs. NCBI nr
Match: KAF4390631.1 (hypothetical protein G4B88_015521, partial [Cannabis sativa])

HSP 1 Score: 1550.0 bits (4012), Expect = 0.0e+00
Identity = 822/1317 (62.41%), Postives = 978/1317 (74.26%), Query Frame = 0

Query: 11   EKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTL 70
            +K+DSW+T+ TLAYQ+LG+VYGDLSTSPLYVYKSTFA+++QHS+TNEE+YGVL FVFWTL
Sbjct: 13   KKKDSWKTVLTLAYQSLGIVYGDLSTSPLYVYKSTFAEDIQHSDTNEEIYGVLSFVFWTL 72

Query: 71   TLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYK-RDTPEP 130
            TLIPL+KYVFIVL+A+DNGEGGTFALYSLLCRHAR+SSLPN Q+AD++LS Y  +D    
Sbjct: 73   TLIPLVKYVFIVLRANDNGEGGTFALYSLLCRHARVSSLPNCQVADQDLSEYNTKDGVVS 132

Query: 131  TSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAK 190
             +  +    LK+TLEK +VLQ +LLVLALIGTCMVIGDGVLTPAIS              
Sbjct: 133  PTKTTAGLTLKSTLEKYRVLQTVLLVLALIGTCMVIGDGVLTPAIS-------------- 192

Query: 191  EHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVY 250
                 ++VP+ACA+L+FLFALQHYGTH+VGFLFAP+VI WL CISAIG+YNI +WNP VY
Sbjct: 193  ----DVEVPVACAVLIFLFALQHYGTHKVGFLFAPIVITWLFCISAIGVYNIIHWNPHVY 252

Query: 251  KALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSL 310
            +ALSPYYMYKFLKKT++ GWM+LGG+LLCITGSEAMFADLGHFS  SI++AFTFVVYPSL
Sbjct: 253  QALSPYYMYKFLKKTQRGGWMSLGGILLCITGSEAMFADLGHFSQLSIQIAFTFVVYPSL 312

Query: 311  VLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSII 370
            +LAYMGQAAYLS HH+L+ DYRIGFYVSVPEK+RWPVLAIAILAAVVGSQA+ITGTFSII
Sbjct: 313  ILAYMGQAAYLSMHHDLETDYRIGFYVSVPEKIRWPVLAIAILAAVVGSQAVITGTFSII 372

Query: 371  KQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVI 430
            KQC++LGCFP+VKIVHTSSK+HGQIYIP INW LM+LCL+VTIGFR+T+R+GNA+GLAVI
Sbjct: 373  KQCTALGCFPKVKIVHTSSKVHGQIYIPEINWTLMLLCLSVTIGFRDTKRMGNASGLAVI 432

Query: 431  TVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLT 490
            TVMLV+TCLMSLV+VLCWHKS+ LAI F+ F GSIEALYFSASL+KF EGAWVP+ALS  
Sbjct: 433  TVMLVTTCLMSLVMVLCWHKSIFLAICFIFFFGSIEALYFSASLIKFREGAWVPVALSFI 492

Query: 491  FLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFS 550
            FLVVMYVWHYGT KKYE+DV+NKV INWLLG+GP +GIVRVRGIGL+HTELVSGIP++FS
Sbjct: 493  FLVVMYVWHYGTQKKYESDVQNKVSINWLLGLGPNLGIVRVRGIGLIHTELVSGIPAVFS 552

Query: 551  HFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDD 610
            HFVTN+PAFH+++VFLC+KSV +PHVRPEERFLVGRVGPKEYRLYRCIARYGY D+HKDD
Sbjct: 553  HFVTNLPAFHQVVVFLCVKSVPVPHVRPEERFLVGRVGPKEYRLYRCIARYGYHDIHKDD 612

Query: 611  LEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCEN-ES 670
            LEFE+DL  SIAEFIRSE+ EC+      L++++ +E+M V+GT+S+ L GI++ E+   
Sbjct: 613  LEFEKDLVRSIAEFIRSERPECDM----RLEESESDEKMTVVGTASSNLKGIRLSEDGGD 672

Query: 671  CSLPIIDSS---EIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIMGHS 730
            CS     S+   EIRS +  RKRVRFV+PES ++D +T++ELQELMEAREAGMAFI+GHS
Sbjct: 673  CSERAGTSAELQEIRSPKKVRKRVRFVLPESPRIDRETQKELQELMEAREAGMAFILGHS 732

Query: 731  YVKAKKGS-------------------EAKTGQLSFPLYSTLPLYVYLQSPKFLRVFPPQ 790
            YV+AK GS                      +  LSFP  STL + +         V    
Sbjct: 733  YVRAKSGSGFMKRLVINIGYDFLRRNFRGPSYALSFPHASTLEVGMVYHKRWHNNVCGVS 792

Query: 791  SSSFSTMARISLAFALVFLLLFGGVSPASASPPGKIVRGVVSNVVSSLVKKLWSMKSTAK 850
              S +TMAR   A  L+ ++   G +  SA+PP KIV G+VSNVVS+LVK LWS+KST K
Sbjct: 793  YLSGTTMARSLTALLLILVIFSSGYASVSATPPAKIVSGIVSNVVSALVKWLWSLKSTPK 852

Query: 851  TAVSSRSMMKFESGYTVETVFDGSKLGIDPYSVEMSPSGELLILDAENSNIYKISMPLSR 910
            TA+SSRSMMKFESGYTVETVFDGSKLGI+PYSVE+SPSG+LL+LD+EN+           
Sbjct: 853  TAISSRSMMKFESGYTVETVFDGSKLGIEPYSVEVSPSGQLLVLDSENN----------- 912

Query: 911  YSRPKLVSGSAEGYPGHVDGHPREARMNHPKGLTLDERGNIYIADTMNMAIRKISDTGVT 970
             SRPKLV+GS EGY GHVDG PREAR+NHPKGLT D++GNIYIADTMNMAIRKISDTGVT
Sbjct: 913  -SRPKLVAGSPEGYAGHVDGKPREARLNHPKGLTADDKGNIYIADTMNMAIRKISDTGVT 972

Query: 971  TIAGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGSSCSLLVIDRGNKAIREIELNYDDCNT 1030
            TIAGG+W QG  H+DGPSEDAKFS+DFDV+Y+GSSCSLL+IDRGN+AIREI+LN DDC+ 
Sbjct: 973  TIAGGKWGQGGDHVDGPSEDAKFSDDFDVIYMGSSCSLLIIDRGNQAIREIQLNDDDCSY 1032

Query: 1031 QYADSLNLGVVLLVAAGLFGYLLALLQRRVQAVFSSPKDEDVKSHQMKKAAQAAQYQRPP 1090
            QY  S +LG+ +LVAA  FGY+LALLQRRV+A+FSS   EDV++  +K+    + YQ+PP
Sbjct: 1033 QYDSSFHLGIAMLVAAAFFGYMLALLQRRVRAMFSS--QEDVRT-PIKRTMPMSPYQKPP 1092

Query: 1091 LKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSGSSVADIVGGFLPGFRRKPLNNQIHQQ 1150
             KSVRP LIP+EDE E+ EEG FGS GRL +N+GSS+A+I GG   GFR KP + QI  Q
Sbjct: 1093 -KSVRPPLIPAEDEYERSEEGIFGSFGRLVLNTGSSMAEIFGGLFSGFRSKPRHYQIQHQ 1152

Query: 1151 FQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPTIKKTYPFMTQDLDRSHQFKPNRNYFN 1210
            F Q +++ NSWP+QESF                                           
Sbjct: 1153 FHQQSKYSNSWPMQESF------------------------------------------- 1163

Query: 1211 GWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQIQQQQQQMQHHQQHHYQQYQQYHHRQY 1270
                                                                        
Sbjct: 1213 ------------------------------------------------------------ 1163

Query: 1271 SAGPTTYYEKNCETKEIVFGAVQEQDGRREAMVIKAVDYGDPRYNHHNIRARYNYTG 1304
                                     DGRREA+VIKAVDYGDP YNHHNIR R NYTG
Sbjct: 1273 -------------------------DGRREAVVIKAVDYGDPVYNHHNIRPRLNYTG 1163

BLAST of CmaCh04G027160 vs. NCBI nr
Match: KAG6398247.1 (hypothetical protein SASPL_139702 [Salvia splendens])

HSP 1 Score: 1503.4 bits (3891), Expect = 0.0e+00
Identity = 812/1334 (60.87%), Postives = 981/1334 (73.54%), Query Frame = 0

Query: 10   PEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWT 69
            P KR+SWRT+ TLAYQ+LGVVYGDLSTSPLYVYKSTFA+++QHSETNEE+YGVL FVFWT
Sbjct: 21   PIKRESWRTVLTLAYQSLGVVYGDLSTSPLYVYKSTFAEDIQHSETNEEIYGVLSFVFWT 80

Query: 70   LTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEP 129
            LTLIPLLKYVFIVL+ADDNGEGGTFALYSLLCRHAR+S+LPN QLADE+L  YK+D    
Sbjct: 81   LTLIPLLKYVFIVLRADDNGEGGTFALYSLLCRHARVSTLPNGQLADEDLYEYKKDGIAS 140

Query: 130  TSNFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAK 189
             +       L++TLEK +VLQR+LL+LAL GTCMVIGDGVLTPAISVFSAVSG+EL ++K
Sbjct: 141  GNFRGLGLRLRSTLEKHRVLQRMLLILALFGTCMVIGDGVLTPAISVFSAVSGLELVVSK 200

Query: 190  EHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVY 249
             HHQYI+VP+AC ILVFLF LQHYGTHR+G LFAPVVI WL CISAIG+YNI +WNP VY
Sbjct: 201  HHHQYIEVPVACVILVFLFYLQHYGTHRIGLLFAPVVITWLFCISAIGVYNIIHWNPHVY 260

Query: 250  KALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSL 309
            KALSPYYMYKFL+KT++ GWM+LGG+LLCITGSEAMFADLGHFS  SIK+AF+ VVYPSL
Sbjct: 261  KALSPYYMYKFLRKTQRDGWMSLGGILLCITGSEAMFADLGHFSQMSIKIAFSCVVYPSL 320

Query: 310  VLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSII 369
            +LAYMGQAAYLSKHH  ++DY+IGFYVSVPEK+RWPVLAIAILAAVVGSQAIITGTFSII
Sbjct: 321  ILAYMGQAAYLSKHHINESDYQIGFYVSVPEKLRWPVLAIAILAAVVGSQAIITGTFSII 380

Query: 370  KQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVI 429
            KQ                                       TIGFR+T+ + NA+GLAVI
Sbjct: 381  KQ--------------------------------------FTIGFRDTKHISNASGLAVI 440

Query: 430  TVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLT 489
            TVMLV+TCLMSLVI+LCW +SVL A+ F++F GSIEALYFSASL+KF EGAWVPIALS T
Sbjct: 441  TVMLVTTCLMSLVIILCWKRSVLFALGFILFFGSIEALYFSASLIKFREGAWVPIALSFT 500

Query: 490  FLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFS 549
            FL +MY WHYGTLKKYE DV+NKV INWLLG+ P +GIVRVRGIGL+HTELVSGIP+IFS
Sbjct: 501  FLAIMYTWHYGTLKKYEFDVQNKVSINWLLGLSPNLGIVRVRGIGLIHTELVSGIPAIFS 560

Query: 550  HFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDD 609
            HFVTN+PAFH++LV  CIK V +PHVR +ERFLVGRVGPKEYR+YRCIARYGYRD   DD
Sbjct: 561  HFVTNLPAFHQVLVLFCIKYVPVPHVRSDERFLVGRVGPKEYRVYRCIARYGYRDTIIDD 620

Query: 610  LEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCE---- 669
            +EFE+DL CSIAEFIRSE +ECN    + L   ++ E+M VIGTSS+   G+++ E    
Sbjct: 621  VEFEKDLVCSIAEFIRSEVSECNGSSEHTL---ENEEKMTVIGTSSSHAKGVKLWEDDGG 680

Query: 670  -----NESCSLPIIDSSEIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAF 729
                 ++  +    +  EIRS    RKRVRF+VPES +++ + R EL+ELMEAREAGMAF
Sbjct: 681  GDLQSSDDATTSTNELGEIRSPVGPRKRVRFLVPESPEIEKEERTELRELMEAREAGMAF 740

Query: 730  IMGHSYVKAKKGSEAKTGQLSFPLYSTLPLYVYLQSPKFLRVFPPQSS-SFSTMARIS-L 789
            I+GH YVKAK GS      +    Y  L      ++P +   FP  S+     M R +  
Sbjct: 741  ILGHCYVKAKTGSSLMKRLVVNVGYDFLRR--NSRAPTYASSFPRASTLEVGEMGRKNCF 800

Query: 790  AFALVFLLLFGGVSPASASPPGKIVRGVVSNVVSSLVKKLWSMKSTAKTAVSSRSMMKFE 849
              A++ L+   G S  SASP  KIV GVV+ VVS++ K+LWS+KS  KT+ SSRSMMKFE
Sbjct: 801  VLAVILLIFLAGCSSVSASPATKIVGGVVTKVVSTIFKRLWSLKSAIKTSTSSRSMMKFE 860

Query: 850  SGYTVETVFDGSKLGIDPYSVEMSPSGELLILDAENSNIYKISMPLSRY----------- 909
             GYTVETVFDGSK GI+PY+VE+SP  E+LILD+ NSNIYK+  PLSR+           
Sbjct: 861  GGYTVETVFDGSKHGIEPYTVEISPDEEVLILDSLNSNIYKVPTPLSRFDGLVLLVITSC 920

Query: 910  -----------SRPKLVSGSAEGYPGHVDGHPREARMNHPKGLTLDERGNIYIADTMNMA 969
                       SRPKL++GSAEGY GHVDG  R AR+NHP+GLT+D+ GNIY+ADTMNMA
Sbjct: 921  VAFEVKVVIFDSRPKLLAGSAEGYNGHVDGKLRHARLNHPRGLTVDDSGNIYVADTMNMA 980

Query: 970  IRKISDTGVTTIAGGRWNQGSGHIDGPSEDAKFSNDFDVVYVGSSCSLLVIDRGNKAIRE 1029
            IRKISD+GV TIAGG+  +G GHIDGPSEDA+FS+DFD+VYVGS+CSLLVIDRGN+AIRE
Sbjct: 981  IRKISDSGVVTIAGGKSGRGGGHIDGPSEDARFSDDFDLVYVGSTCSLLVIDRGNQAIRE 1040

Query: 1030 IELNYDDCNTQYADSLNLGVVLLVAAGLFGYLLALLQRRVQAVFSSPKDEDVKSHQMKKA 1089
            I+L+  DC+ +  ++L+LG+ +L AA  FGY+LALLQRRV ++ S    ++ K +     
Sbjct: 1041 IQLHEHDCSHEEDNNLDLGIAVLAAACFFGYMLALLQRRVASMLSYDNPKEPKPYATGVP 1100

Query: 1090 AQAAQYQRPPLKSVRPSLIPSEDEPEKLEEGFFGSLGRLFVNSGSSVADIVGGFLPGFRR 1149
                Q Q P  KSV P LIP+EDE +K E+G F SLG+L V++GSSVA++ GG   GF++
Sbjct: 1101 PAPYQRQAPHAKSVLPPLIPTEDEYDKQEDGLFTSLGKLVVHTGSSVAELFGGLFSGFKK 1160

Query: 1150 KPL--------NNQIHQQFQQVNRHPNSWPLQESFVIPDEDEPPSIETKTPTIKKTYPFM 1209
            KP+         +Q H   Q  +RH  +WP+QESFV+P EDEPP +E + PT +K YP+ 
Sbjct: 1161 KPVVINHMPAYQHQQHHHHQYQSRHGGAWPVQESFVVPHEDEPPPLEAREPTPRKNYPY- 1220

Query: 1210 TQDLDRSHQFKPNRNYFNGWDNGGEFHQQQQQPQQIHHQQQQIQQQPQQQQIQQQQQQMQ 1269
             +D ++    K  R Y+N W++ G  H+ QQ   Q H                      Q
Sbjct: 1221 -KDAEKMRLSKQGRPYYNQWNDHG--HEYQQPQLQSH----------------------Q 1273

Query: 1270 HHQQHHYQQYQQYHHRQYSAGPTTYYE-KNCETKEIVFGAVQEQDGRREAMVIKAVDYGD 1302
            HHQ+H              A P TYYE +NCET EIVFGAVQEQ+GRREAMVIKAVDY D
Sbjct: 1281 HHQKHR------------PASPQTYYEQQNCETNEIVFGAVQEQEGRREAMVIKAVDYTD 1273

BLAST of CmaCh04G027160 vs. TAIR 10
Match: AT1G70300.1 (K+ uptake permease 6 )

HSP 1 Score: 1067.4 bits (2759), Expect = 9.3e-312
Identity = 530/732 (72.40%), Postives = 637/732 (87.02%), Query Frame = 0

Query: 12  KRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTLT 71
           K++SWRT+ TLAYQ+LGVVYGDLS SPLYVYKSTFA+++ HSE+NEE++GVL F+FWT+T
Sbjct: 12  KKESWRTVLTLAYQSLGVVYGDLSISPLYVYKSTFAEDIHHSESNEEIFGVLSFIFWTIT 71

Query: 72  LIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEPTS 131
           L+PLLKYVFIVL+ADDNGEGGTFALYSLLCRHAR++SLP+ QLADE+L  YK D+   +S
Sbjct: 72  LVPLLKYVFIVLRADDNGEGGTFALYSLLCRHARVNSLPSCQLADEQLIEYKTDSIGSSS 131

Query: 132 --NFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAK 191
                F++ LK+TLEK  VLQ+ILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSM+K
Sbjct: 132 MPQSGFAASLKSTLEKHGVLQKILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMSK 191

Query: 192 EHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVY 251
           EHH+YI++P AC IL+ LFALQHYGTHRVGFLFAPV+++WL+CISAIG+YNIF+WNP VY
Sbjct: 192 EHHKYIELPAACVILIGLFALQHYGTHRVGFLFAPVILLWLMCISAIGVYNIFHWNPHVY 251

Query: 252 KALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSL 311
           +ALSPYYMYKFLKKT+ +GWM+LGG+LLCITGSEAMFADLGHFS  SIK+AFT +VYPSL
Sbjct: 252 QALSPYYMYKFLKKTQSRGWMSLGGILLCITGSEAMFADLGHFSQLSIKIAFTSLVYPSL 311

Query: 312 VLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSII 371
           +LAYMGQAAYLS+HH ++++Y IGFYVSVPE++RWPVL IAILAAVVGSQAIITGTFSII
Sbjct: 312 ILAYMGQAAYLSQHHIIESEYNIGFYVSVPERLRWPVLVIAILAAVVGSQAIITGTFSII 371

Query: 372 KQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVI 431
           KQCS+LGCFP+VKIVHTSSKIHGQIYIP INWILM+LCLAVTIGFR+T+RLGNA+GLAVI
Sbjct: 372 KQCSALGCFPKVKIVHTSSKIHGQIYIPEINWILMVLCLAVTIGFRDTKRLGNASGLAVI 431

Query: 432 TVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLT 491
           TVMLV+TCLMSLVIVLCWHKSV+ AI F+VF G+IE+LYFSASL+KF EGAWVPIAL+  
Sbjct: 432 TVMLVTTCLMSLVIVLCWHKSVIFAIVFVVFFGTIESLYFSASLIKFLEGAWVPIALAFC 491

Query: 492 FLVVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFS 551
           FL+ M  WHYGTLK+YE DV+NKV +NWLL +   +GI RVRG+GL+HTELVSG+P+IFS
Sbjct: 492 FLLAMCTWHYGTLKRYEYDVQNKVSVNWLLSLSQTLGIARVRGLGLIHTELVSGVPAIFS 551

Query: 552 HFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDD 611
           HFVTN+PAFH++LVFLC+KSV +PHVRP+ERFLVGR+GPKE+R+YRCI R+GYRDVHKDD
Sbjct: 552 HFVTNLPAFHQVLVFLCVKSVPVPHVRPQERFLVGRIGPKEFRIYRCIVRFGYRDVHKDD 611

Query: 612 LEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQ-MCENES 671
            EFE DL CSIAEFIR+E     T       + +D++RM V+GT ST + GI+   E++ 
Sbjct: 612 FEFEGDLVCSIAEFIRTEAETAATAAET---NGEDDDRMSVVGTCSTYMQGIEDHYESDI 671

Query: 672 CSLPIIDSSEIRSERLR-------RKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFI 731
                  +SEIRS + +       +KRVRFVVPE+ K++ +TR+EL EL EARE G+A+I
Sbjct: 672 DDPDKPGTSEIRSPKPKKKSKSKVKKRVRFVVPETPKIEKETRQELMELTEAREGGVAYI 731

Query: 732 MGHSYVKAKKGS 734
           MG++Y+KAK GS
Sbjct: 732 MGNAYMKAKPGS 740

BLAST of CmaCh04G027160 vs. TAIR 10
Match: AT5G14880.1 (Potassium transporter family protein )

HSP 1 Score: 1059.3 bits (2738), Expect = 2.5e-309
Identity = 527/731 (72.09%), Postives = 626/731 (85.64%), Query Frame = 0

Query: 10  PEKRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWT 69
           P K++SW T+ TLAYQ+LGVVYGDL+TSPLYVYKSTFA+++ HSETNEE++GVL  +FWT
Sbjct: 11  PVKKESWWTVLTLAYQSLGVVYGDLATSPLYVYKSTFAEDITHSETNEEIFGVLSLIFWT 70

Query: 70  LTLIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEP 129
           LTLIPL+KYVFIVL+ADDNGEGGTFALYSLLCRHAR+SSLPN QLADE+LS YK+++ E 
Sbjct: 71  LTLIPLVKYVFIVLRADDNGEGGTFALYSLLCRHARISSLPNFQLADEDLSEYKKNSGEN 130

Query: 130 TSNFSFSS-CLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMA 189
                     LK TLEK K LQ +LLVLALIGTCMVIGDGVLTPAISVFSAVSG+ELSM+
Sbjct: 131 PMRLKVPGWSLKNTLEKHKFLQNMLLVLALIGTCMVIGDGVLTPAISVFSAVSGLELSMS 190

Query: 190 KEHHQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLV 249
           K+ HQY++VP+ CAIL+ LF+LQHYGTHR+GF+FAP+V+ WLLCIS IG+YNIF+WNP V
Sbjct: 191 KQQHQYVEVPVVCAILILLFSLQHYGTHRLGFVFAPIVLAWLLCISTIGVYNIFHWNPHV 250

Query: 250 YKALSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPS 309
           YKALSPYY+YKFLKKT+K+GWM+LGG+LLCITGSEAMFADLGHF+  SI++AFTF VYPS
Sbjct: 251 YKALSPYYIYKFLKKTRKRGWMSLGGILLCITGSEAMFADLGHFTQLSIQIAFTFAVYPS 310

Query: 310 LVLAYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSI 369
           L+LAYMGQAAYLSKHH L +DYRIGFYVSVPE++RWPVLAIAILAAVVGSQAIITGTFSI
Sbjct: 311 LILAYMGQAAYLSKHHVLQSDYRIGFYVSVPEQIRWPVLAIAILAAVVGSQAIITGTFSI 370

Query: 370 IKQCSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAV 429
           IKQC+SLGCFP+VKIVHTSS++HGQIYIP INW LM+LCLAVT+GFR+T+ + NA+GLAV
Sbjct: 371 IKQCTSLGCFPKVKIVHTSSRMHGQIYIPEINWTLMLLCLAVTVGFRDTKHISNASGLAV 430

Query: 430 ITVMLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSL 489
           ITVMLV+TCLMSLVIVLCW KS L A+AF+ F G+IE LYFSASL+KF EGAWVP+ALS 
Sbjct: 431 ITVMLVTTCLMSLVIVLCWRKSSLYALAFIFFFGTIEVLYFSASLIKFLEGAWVPVALSF 490

Query: 490 TFLVVMYVWHYGTLKKYEADVENKVPINWLLGI--GPKIGIVRVRGIGLVHTELVSGIPS 549
            FL++MYVWHYGTLK+YE DV+NKV INWLL +     +GIVRV GIG+++TELVSGIP+
Sbjct: 491 IFLLIMYVWHYGTLKRYEFDVQNKVSINWLLTLFGSSNLGIVRVHGIGVINTELVSGIPA 550

Query: 550 IFSHFVTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVH 609
           IFSHF+TN+PAFH+++VFLC+KSV +PHV+PEERFLVGRVGPKEYRLYRCIARYGYRDVH
Sbjct: 551 IFSHFITNLPAFHQVVVFLCVKSVPVPHVKPEERFLVGRVGPKEYRLYRCIARYGYRDVH 610

Query: 610 KDDLEFERDLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCEN 669
           KDD+EFE DL CSIAEFIRS+K     +  +  +++  NER+ V+  SS+ L G+Q+ E+
Sbjct: 611 KDDVEFENDLICSIAEFIRSDKP--LNYSPDPENESGINERLTVVAASSSNLEGVQIYED 670

Query: 670 ESCSLPIIDSSE----IRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIM 729
           +        SS     +      +KRVRFV+PES ++D     EL EL EAREAGMAFIM
Sbjct: 671 DGSDKQEPSSSSEVIMVAPSPRFKKRVRFVLPESARIDRSAEEELTELTEAREAGMAFIM 730

Query: 730 GHSYVKAKKGS 734
           GHSYV+AK GS
Sbjct: 731 GHSYVRAKSGS 739

BLAST of CmaCh04G027160 vs. TAIR 10
Match: AT2G40540.1 (potassium transporter 2 )

HSP 1 Score: 923.7 bits (2386), Expect = 1.7e-268
Identity = 467/743 (62.85%), Postives = 587/743 (79.00%), Query Frame = 0

Query: 12  KRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTLT 71
           K++SWR++  LAYQ+LGVVYGDLS SPLYV+KSTFA+++QHSETNEE+YGV+ FVFWTLT
Sbjct: 15  KKESWRSVLLLAYQSLGVVYGDLSISPLYVFKSTFAEDIQHSETNEEIYGVMSFVFWTLT 74

Query: 72  LIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEPTS 131
           L+PLLKYVFIVL+ADDNGEGGTFALYSL+CRH ++S LPN+Q++DE LS YK + P P  
Sbjct: 75  LVPLLKYVFIVLRADDNGEGGTFALYSLICRHVKVSLLPNRQVSDEALSTYKLEHP-PEK 134

Query: 132 NFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAKEH 191
           N    SC+K  LEK K L   LL+L L+GTCMVIGDG+LTPAISVFSAVSG+EL+M+KEH
Sbjct: 135 N--HDSCVKRYLEKHKWLHTALLLLVLLGTCMVIGDGLLTPAISVFSAVSGLELNMSKEH 194

Query: 192 HQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVYKA 251
           HQY  +P+ C ILV LF+LQH+GTHRVGF+FAP+V+ WLLCIS IGLYNI  WNP +YKA
Sbjct: 195 HQYAVIPITCFILVCLFSLQHFGTHRVGFVFAPIVLTWLLCISGIGLYNIIQWNPHIYKA 254

Query: 252 LSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSLVL 311
           LSP YM+ FL+KT+  GWM+LGG+LLCITG+EAMFADLGHF++ +I++AFTF+VYP+L+L
Sbjct: 255 LSPTYMFMFLRKTRVSGWMSLGGILLCITGAEAMFADLGHFNYAAIQIAFTFLVYPALIL 314

Query: 312 AYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSIIKQ 371
           AYMGQAAYLS+HH+  + + IGFYVSVP+ + WPVLA+AILA+VVGSQAII+GTFSII Q
Sbjct: 315 AYMGQAAYLSRHHH--SAHAIGFYVSVPKCLHWPVLAVAILASVVGSQAIISGTFSIINQ 374

Query: 372 CSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVITV 431
             SLGCFPRVK++HTS K+HGQIYIP INW+LMILC+AVTIGFR+ + LGNA+GLAV+ V
Sbjct: 375 SQSLGCFPRVKVIHTSDKMHGQIYIPEINWMLMILCIAVTIGFRDVKHLGNASGLAVMAV 434

Query: 432 MLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLTFL 491
           MLV+TCL SLVIVLCWHK  +LA+AFL+F GSIE LYFSASL KF EGAW+PI LSL F+
Sbjct: 435 MLVTTCLTSLVIVLCWHKPPILALAFLLFFGSIELLYFSASLTKFREGAWLPILLSLIFM 494

Query: 492 VVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFSHF 551
           ++M+VWHY T+KKYE D++NKV + WLL +GP +GI RV GIGLV T+L SGIP+ FS F
Sbjct: 495 IIMFVWHYTTIKKYEFDLQNKVSLEWLLALGPSLGISRVPGIGLVFTDLTSGIPANFSRF 554

Query: 552 VTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDLE 611
           VTN+PAFH +LVF+C+KSV +P V P ER+LVGRVGP ++R YRCI RYGYRDVH+D   
Sbjct: 555 VTNLPAFHRVLVFVCVKSVPVPFVPPAERYLVGRVGPVDHRSYRCIVRYGYRDVHQDVDS 614

Query: 612 FERDLACSIAEFIRSE-----KTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCEN 671
           FE +L   +A+FIR +     + E +  R    +++    R+ VIGT + ++      E+
Sbjct: 615 FETELVSKLADFIRYDWHKRTQQEDDNARSVQSNESSSESRLAVIGTVAYEIEDNLQPES 674

Query: 672 ESCSLPIIDSSEIRSERLRR------KRVRFVVPE----------STKMDVDTRRELQEL 731
            S     ++S E   +          +RVRF V E          S + D + R EL++L
Sbjct: 675 VSIGFSTVESMEDVIQMAEPAPTATIRRVRFAVEENSYEDEGSTSSAEADAELRSELRDL 734

Query: 732 MEAREAGMAFIMGHSYVKAKKGS 734
           + A+EAG AFI+GHS+VKAK+GS
Sbjct: 735 LAAQEAGTAFILGHSHVKAKQGS 752

BLAST of CmaCh04G027160 vs. TAIR 10
Match: AT2G40540.2 (potassium transporter 2 )

HSP 1 Score: 923.7 bits (2386), Expect = 1.7e-268
Identity = 467/743 (62.85%), Postives = 587/743 (79.00%), Query Frame = 0

Query: 12  KRDSWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTLT 71
           K++SWR++  LAYQ+LGVVYGDLS SPLYV+KSTFA+++QHSETNEE+YGV+ FVFWTLT
Sbjct: 15  KKESWRSVLLLAYQSLGVVYGDLSISPLYVFKSTFAEDIQHSETNEEIYGVMSFVFWTLT 74

Query: 72  LIPLLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEPTS 131
           L+PLLKYVFIVL+ADDNGEGGTFALYSL+CRH ++S LPN+Q++DE LS YK + P P  
Sbjct: 75  LVPLLKYVFIVLRADDNGEGGTFALYSLICRHVKVSLLPNRQVSDEALSTYKLEHP-PEK 134

Query: 132 NFSFSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAKEH 191
           N    SC+K  LEK K L   LL+L L+GTCMVIGDG+LTPAISVFSAVSG+EL+M+KEH
Sbjct: 135 N--HDSCVKRYLEKHKWLHTALLLLVLLGTCMVIGDGLLTPAISVFSAVSGLELNMSKEH 194

Query: 192 HQYIKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVYKA 251
           HQY  +P+ C ILV LF+LQH+GTHRVGF+FAP+V+ WLLCIS IGLYNI  WNP +YKA
Sbjct: 195 HQYAVIPITCFILVCLFSLQHFGTHRVGFVFAPIVLTWLLCISGIGLYNIIQWNPHIYKA 254

Query: 252 LSPYYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSLVL 311
           LSP YM+ FL+KT+  GWM+LGG+LLCITG+EAMFADLGHF++ +I++AFTF+VYP+L+L
Sbjct: 255 LSPTYMFMFLRKTRVSGWMSLGGILLCITGAEAMFADLGHFNYAAIQIAFTFLVYPALIL 314

Query: 312 AYMGQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSIIKQ 371
           AYMGQAAYLS+HH+  + + IGFYVSVP+ + WPVLA+AILA+VVGSQAII+GTFSII Q
Sbjct: 315 AYMGQAAYLSRHHH--SAHAIGFYVSVPKCLHWPVLAVAILASVVGSQAIISGTFSIINQ 374

Query: 372 CSSLGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVITV 431
             SLGCFPRVK++HTS K+HGQIYIP INW+LMILC+AVTIGFR+ + LGNA+GLAV+ V
Sbjct: 375 SQSLGCFPRVKVIHTSDKMHGQIYIPEINWMLMILCIAVTIGFRDVKHLGNASGLAVMAV 434

Query: 432 MLVSTCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLTFL 491
           MLV+TCL SLVIVLCWHK  +LA+AFL+F GSIE LYFSASL KF EGAW+PI LSL F+
Sbjct: 435 MLVTTCLTSLVIVLCWHKPPILALAFLLFFGSIELLYFSASLTKFREGAWLPILLSLIFM 494

Query: 492 VVMYVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFSHF 551
           ++M+VWHY T+KKYE D++NKV + WLL +GP +GI RV GIGLV T+L SGIP+ FS F
Sbjct: 495 IIMFVWHYTTIKKYEFDLQNKVSLEWLLALGPSLGISRVPGIGLVFTDLTSGIPANFSRF 554

Query: 552 VTNIPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDLE 611
           VTN+PAFH +LVF+C+KSV +P V P ER+LVGRVGP ++R YRCI RYGYRDVH+D   
Sbjct: 555 VTNLPAFHRVLVFVCVKSVPVPFVPPAERYLVGRVGPVDHRSYRCIVRYGYRDVHQDVDS 614

Query: 612 FERDLACSIAEFIRSE-----KTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCEN 671
           FE +L   +A+FIR +     + E +  R    +++    R+ VIGT + ++      E+
Sbjct: 615 FETELVSKLADFIRYDWHKRTQQEDDNARSVQSNESSSESRLAVIGTVAYEIEDNLQPES 674

Query: 672 ESCSLPIIDSSEIRSERLRR------KRVRFVVPE----------STKMDVDTRRELQEL 731
            S     ++S E   +          +RVRF V E          S + D + R EL++L
Sbjct: 675 VSIGFSTVESMEDVIQMAEPAPTATIRRVRFAVEENSYEDEGSTSSAEADAELRSELRDL 734

Query: 732 MEAREAGMAFIMGHSYVKAKKGS 734
           + A+EAG AFI+GHS+VKAK+GS
Sbjct: 735 LAAQEAGTAFILGHSHVKAKQGS 752

BLAST of CmaCh04G027160 vs. TAIR 10
Match: AT2G30070.1 (potassium transporter 1 )

HSP 1 Score: 756.9 bits (1953), Expect = 2.7e-218
Identity = 394/719 (54.80%), Postives = 505/719 (70.24%), Query Frame = 0

Query: 15  SWRTIFTLAYQALGVVYGDLSTSPLYVYKSTFAKNLQHSETNEEVYGVLCFVFWTLTLIP 74
           S   + TLAYQ+LGV+YGDLSTSPLYVYK+TF+  L   E +EE++GV  F+FWT TLI 
Sbjct: 21  SCANVLTLAYQSLGVIYGDLSTSPLYVYKTTFSGKLSLHEDDEEIFGVFSFIFWTFTLIA 80

Query: 75  LLKYVFIVLKADDNGEGGTFALYSLLCRHARLSSLPNQQLADEELSAYKRDTPEPTSNFS 134
           L KYVFIVL ADDNGEGGTFALYSLLCR+A+LS LPN Q  DE+LS Y   +P  T    
Sbjct: 81  LFKYVFIVLSADDNGEGGTFALYSLLCRYAKLSILPNHQEMDEKLSTYATGSPGETRQ-- 140

Query: 135 FSSCLKATLEKCKVLQRILLVLALIGTCMVIGDGVLTPAISVFSAVSGVELSMAKEHHQY 194
            S+ +K+  EK    Q+ LL+  L+GTCM IGD VLTP ISV SAVSGV+L +   H  Y
Sbjct: 141 -SAAVKSFFEKHPKSQKCLLLFVLLGTCMAIGDSVLTPTISVLSAVSGVKLKIPNLHENY 200

Query: 195 IKVPLACAILVFLFALQHYGTHRVGFLFAPVVIVWLLCISAIGLYNIFYWNPLVYKALSP 254
           + V +AC ILV +F++Q YGTHRV F+FAP+   WLL IS+IG+YN   WNP +  ALSP
Sbjct: 201 V-VIIACIILVAIFSVQRYGTHRVAFIFAPISTAWLLSISSIGVYNTIKWNPRIVSALSP 260

Query: 255 YYMYKFLKKTKKQGWMALGGVLLCITGSEAMFADLGHFSHFSIKLAFTFVVYPSLVLAYM 314
            YMYKFL+ T  +GW++LGGV+L ITG E MFADLGHFS  SIK+AF+F VYP L+LAYM
Sbjct: 261 VYMYKFLRSTGVEGWVSLGGVVLSITGVETMFADLGHFSSLSIKVAFSFFVYPCLILAYM 320

Query: 315 GQAAYLSKHHNLDNDYRIGFYVSVPEKVRWPVLAIAILAAVVGSQAIITGTFSIIKQCSS 374
           G+AA+LSKHH    D +  FY ++PE V WPV  +A  AAVVGSQA+I+ TFSII QC +
Sbjct: 321 GEAAFLSKHH---EDIQQSFYKAIPEPVFWPVFIVATFAAVVGSQAVISATFSIISQCCA 380

Query: 375 LGCFPRVKIVHTSSKIHGQIYIPLINWILMILCLAVTIGFRETERLGNAAGLAVITVMLV 434
           L CFPRVKI+HTSSKIHGQIYIP +NW+LM LCLAVTIG R+T  +G+A GLAV +VMLV
Sbjct: 381 LDCFPRVKIIHTSSKIHGQIYIPEVNWMLMCLCLAVTIGLRDTNMMGHAYGLAVTSVMLV 440

Query: 435 STCLMSLVIVLCWHKSVLLAIAFLVFSGSIEALYFSASLMKFFEGAWVPIALSLTFLVVM 494
           +TCLM+LV+ + W + ++  +AF+VF GSIE LYFS+ + K  EG W+PI LSLTF+ VM
Sbjct: 441 TTCLMTLVMTIVWKQRIITVLAFVVFFGSIELLYFSSCVYKVPEGGWIPILLSLTFMAVM 500

Query: 495 YVWHYGTLKKYEADVENKVPINWLLGIGPKIGIVRVRGIGLVHTELVSGIPSIFSHFVTN 554
           Y+W+YGT KK+E DVENKV ++ ++ +GP IG+VRV GIGLV++ LV+G+P++F HFVTN
Sbjct: 501 YIWNYGTTKKHEFDVENKVSMDRIVSLGPSIGMVRVPGIGLVYSNLVTGVPAVFGHFVTN 560

Query: 555 IPAFHEILVFLCIKSVTMPHVRPEERFLVGRVGPKEYRLYRCIARYGYRDVHKDDLEFER 614
           +PAFH+ILVF+C+KSV +P+V  EERF++ RVGPKEY ++R + RYGYRDV ++  +FE 
Sbjct: 561 LPAFHKILVFVCVKSVQVPYVGEEERFVISRVGPKEYGMFRSVVRYGYRDVPREMYDFES 620

Query: 615 DLACSIAEFIRSEKTECNTFRHNDLDDNDDNERMRVIGTSSTQLNGIQMCENESCSLPII 674
            L  +I EF+ +E                                            P +
Sbjct: 621 RLVSAIVEFVETE--------------------------------------------PGL 670

Query: 675 DSSEIRSERLRRKRVRFVVPESTKMDVDTRRELQELMEAREAGMAFIMGHSYVKAKKGS 734
           +  E+ S  +RRK                + E  E+MEA+EAG+A+I+GHSY KAK+ S
Sbjct: 681 EEEEMSS--VRRK----------------KEECMEIMEAKEAGVAYILGHSYAKAKQSS 670

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W4I41.3e-31072.40Potassium transporter 6 OS=Arabidopsis thaliana OX=3702 GN=POT6 PE=2 SV=1[more]
Q9M7J93.5e-30872.09Potassium transporter 8 OS=Arabidopsis thaliana OX=3702 GN=POT8 PE=2 SV=2[more]
Q67VS57.2e-28565.22Potassium transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK10 PE=2 ... [more]
Q6YWQ42.8e-28168.14Potassium transporter 25 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK25 PE=2 ... [more]
Q5Z6K95.0e-27066.21Potassium transporter 24 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK24 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A498J1T40.0e+0069.78Potassium transporter OS=Malus domestica OX=3750 GN=DVH24_031797 PE=3 SV=1[more]
A0A7J7CZX40.0e+0067.76Potassium transporter OS=Tripterygium wilfordii OX=458696 GN=HS088_TW12G00842 PE... [more]
A0A7J6H6S00.0e+0062.41Potassium transporter OS=Cannabis sativa OX=3483 GN=G4B88_015521 PE=3 SV=1[more]
A0A565CLI00.0e+0061.01Potassium transporter OS=Arabis nemorensis OX=586526 GN=ANE_LOCUS24969 PE=3 SV=1[more]
A0A6J1JNB70.0e+00100.00Potassium transporter OS=Cucurbita maxima OX=3661 GN=LOC111487413 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6602446.10.0e+0094.75Potassium transporter 8, partial [Cucurbita argyrosperma subsp. sororia][more]
RXH89440.10.0e+0069.78hypothetical protein DVH24_031797 [Malus domestica][more]
KAF5739633.10.0e+0067.76potassium transporter 8-like [Tripterygium wilfordii][more]
KAF4390631.10.0e+0062.41hypothetical protein G4B88_015521, partial [Cannabis sativa][more]
KAG6398247.10.0e+0060.87hypothetical protein SASPL_139702 [Salvia splendens][more]
Match NameE-valueIdentityDescription
AT1G70300.19.3e-31272.40K+ uptake permease 6 [more]
AT5G14880.12.5e-30972.09Potassium transporter family protein [more]
AT2G40540.11.7e-26862.85potassium transporter 2 [more]
AT2G40540.21.7e-26862.85potassium transporter 2 [more]
AT2G30070.12.7e-21854.80potassium transporter 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003855Potassium transporterPFAMPF02705K_transcoord: 24..604
e-value: 5.5E-188
score: 625.8
IPR003855Potassium transporterTIGRFAMTIGR00794TIGR00794coord: 22..734
e-value: 3.4E-244
score: 810.3
IPR003855Potassium transporterPANTHERPTHR30540OSMOTIC STRESS POTASSIUM TRANSPORTERcoord: 11..734
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 810..998
e-value: 6.8E-30
score: 106.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1184..1237
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1043..1079
NoneNo IPR availablePANTHERPTHR30540:SF98POTASSIUM TRANSPORTER 6coord: 11..734
NoneNo IPR availableSUPERFAMILY101898NHL repeatcoord: 842..999

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G027160.1CmaCh04G027160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071805 potassium ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015079 potassium ion transmembrane transporter activity