CmaCh04G025910 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G025910
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionTranscription initiation factor IIF subunit beta
LocationCma_Chr04: 17745485 .. 17750314 (-)
RNA-Seq ExpressionCmaCh04G025910
SyntenyCmaCh04G025910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGAAAATGCCTTTCTAACCAAAACCGTTTGTCCCATTTCCTTTCAATTTTTTTCACTTCCGTAGACGTATCATTGCTTTCCTTCCTCTTCTTCTTCTTGTGCTCTCTTCTGCAATGGACGGCGACGACAAACCCATCGACCGTGGCAGTTCCAGAAAACTCCACAGCAATAGCAGCACCGTCGCCGGAACCGACTTCTTGGAGACGAACAAGGCGGACAGAGCAATGTGGCTCTTGAAATGCCCGCAAGTTGTAACTCGCGCCCTTTCCAATTCGCCGGATGATCCTTCTCGTCCGGTCGCTAAGGTTATTGTTTCTGTTGATCCTCTGCAGTCGAATGACGATGATGATTCTTCATCTACTGAGGTATATAATGTCATCTTGCTATGGCGTTTTTGGCTTTGGATTGGGGGCTCTCTTCTTACTTCCTTGTGTGATTTATTGTATTTCTTACTTTCTTTTGCATGTTTTCCATTTTAGTTTGCCTTGTTCATTGCTTCTTGTTTTCATACTATCTTTATCTTAGGGCTTTCTGTTTACTTCTGTGCATGCATTTGCCGTTCTTTTTGTTTATAATTCGTTTTCATTGATTACTCAGTTTAATCTTTTTGTTCCTTTCTTATTTCACTGATTACGGAAACTTTTATTCTTTGTTAAAGTTTCATTGCCGTGTGGTTAGCCTAAATTGCTTTCTCACTGTTACGTTCTCGTGATGCTTTCTGTGAATGGGGTCCTTCTGGTTTACATGCTTGGTTTCCTAACAATCATATGTATTTAATTTGGACTGCGATTTTTAGAACACAGTGGATCAGGAATTTATGGAGATTGGTAAAAATAAAGTAATTTAGTGGTAGGGTTTTCTCACTTTCTACTGACTAAAGGATTTGTACGGGATGTTGTTGGTTATCACTATTGGTCCCCATTTTCTCGTGTTTAACCCGCCCTGAGCCTATGACTGAATGGTTTGGGTCCATGCTTTCCGTCATAAATCTTCCTTGTCTTTTCTTCCATATTTTTTCTAGCCACACTTTTATGTCTACAGTGCCTTATGATGAGGCAAAGGGTCCAAAGTGTTCCCAAATTAAAAACTTCAAATGTTTCCCGACACCGACCTCGTATTCCTAAAGTTATTTGTTGACTAATAAAAAGTTGTGACTTTTTGATTCAAATTCAACTTTATGTAAGTTCTACAAGTTTTCTCACAACCAGATGTTATAGGCTCATGTGATTGTCCCATAAACTTAGTTGAGATGTTTGTAAGTAGACATGGTTGATGTACAAGTATCAAAGAACATAATATCTAAATGAGTAACAATAGAATCTTTAGATTGTGAAATGCTATATGTTCCTAAACTATGTCTCTGTTTAGAATTTATTCACAGCTTTGAATTGGTGAGAGAATTTTTGCCTGTTGGTTTACTAGCGTGTTAAATAAGCAGATGCTCTGTGTAATTGTTGTGACTTGTACATGGTTTTAGCTAAAAATTACTAGACCTTCGGTTTGGCCATGTGCAAGGAGGCCCAGGTACCTTTTAGCCACAATACTATGACAGATTACCTATTTGTAATGCTAGCCATGTCATGTTAATTACTTTCTCTTAAATTATTTTTCTTGTAAGGCACATTTCCAAGATAGTTCTTAGTTATTTCTCCTTTAATAAATTGTGGAGTTATTTGTCATAGAAGATGAATACTTTATCCATGAAGTGAAAACTAATCCATATGTTGCTGAGAGCGCCTTTCAGGGCTTTGGCCTTCCAATTGTGATACAATCTTTTACTACAATTATCTCTAAAGAATGTTTACTCACAGCTCCTGTAATGGTTCTATTATTATTATTTTTTTTAATAAATCAATCACACCAAGGTACAACCTATAGAAACAATCTTATTCCAATAAGGTTGTCGTAATGGTTCTATTATTCGTCCTAACACACTGTCAATTTACTTACCTTTTTAACACTTCTTGTAGATTTTCTTATTCGTCCTAACAGATTGTCAATATGCTGACCTTTTTAACCCTTCTTGTATATTTTCTTACTTGTAAATGCAAGAAACTTTATCTTTTATTTCAAAGTTGGGCTTAAGTTTTTTTTCTCAAGGGTACTTACACTGTTTATTAGTTATACTAAAAGAGAAATTAGAGATTAATTTATATCAGCATCTGTTTGCTTGATTTCTTCAGCTTACAATGGCTCTATGATTTTAGTTTACTATGGAATTGGCTGACACCGACTCCGGAAAAGCATTAAGGGCCTACTCATTGAATATGTCGACAGATTTTATTCCGATGTCTGTATTTTCTGAGTCAGCACAAGGTTGGTTTATGTCGCTCTGGCCTGCTTATTAAAAACTGACCGATGAATATGATGCCTTCAAATGTTTACTTGTCAGTTAGATGAACTATTACTCTGATTGTGATATGAGATTCAATTTATATAATTTGTTCAACTGGTATTGACATCTTCATCAATCTTGGATGTGAATATCTATTTTTATTCTTTAGACTATATGTGCGCCAATAATTATTTTCAAAATCGTCTATACAATATTAATTTTATGGTACTAAACATCATGAGTTTTATCTCTTTCAAATATATTTGAATTAGGAAGCTTTATAGAATTCCTAGGTTTGGGCGTGAGAGATATCTCAATACGCTTCTTGCTGGTTATTTATATAGTGTGACCATTGTTCAAAAAGAAAAAGAAAAGTTCACAATCTGAGAGTTAGTATGAGAGACAGTTTCTTTAGATGAACCTGACTACAGCAAGTTCATTAGGCTTCTGTTTGCTTATATTTCCCTCCAAGTTCCATCCCTTTCTGTGTCCTTTATCTCATCAATCCTCTTTATTCCTTTTCTGTCCAACATCTAGTGTACTGAGATAGGGTAATTGATCTCGAGGCACATATTTTATCTGATGAAAACTAGAACATATTTTCAGCAGTAAGAGATGGAGTAAATAATCTCAAGGCACATGTTGATCAAATTCTGCAGAAAATATTTGTCTTTGCATACGAACAGGGTTCTTAATTATTTGCTGTCTTGACATTTATATGTTCGTTCCAGCTTTTTGAATTGAGATTCTGTAATGTTAGAAGAGGAGATTTACATTGATTGAGTTATAGTATAGAGAACTTCTAATCAAATTTCCATCTTGATCTCTATCTTCAAATTTCTGCTTATTTACTGGACCGACAAATAAAACTGATAAGTTCTGTGTACTATAGAGACTTCAAGAAATCTAGAGCAGCTATAACTTCTATTTTTCATCTTGAAATATTGAAATTCTCTGTGTACAAAGACTTTCAGAAATTTAGAGCAGCTATAACTTTTTTTTATTCTTTGAACTTATTCAACATTATTTTAGATCATTACTACAGAATATCTGCCTGATGGTATCAATTCTTATTTCAATTTAGGGAAGTTTACTGTTGAAGGAAAAATACTGAACAAATTTGACATGAAGCCGCATGATCAAAATCTTGATAGTTACGGGAAACTCTGTCGTGAAAGAACTCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTCTCATTTGTAACTTTCTGCAGCGTACTTGGGTGTTCGGTTATTTCTTTTATGATCTTAGTTCAAATTTCCAAATATTCCATACAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCAATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGTACTTATACTACTTTCTTTTCTACATTGTTTCATATATTCTCATTGTCAATTTCAATTTTGATTTCGCTCGCCGTTTCGGTTTAAGCCTGTGCTATATCTATCTAACCTCGTAGTCAGGCTGAAGCTTGTTCAAATGCTTCCAAGGATTATTTTTTACTATTTGTGTGAACAAATGTGATACAGGAGAAGAAGAAGGTGGTGAGCAAGGGATCGGAAAATAAGAGGCTGAGGAAGGAGCGTGGCGAATTGGAGAAGATCATTTTTAGGCTCTTTGAAAGACAGCCATATTGGACCTCAAAACAATTGATTCAAGAGACCGATCAACCTGAAGTAAGCTTTATCTTGAATTTGCACACTAATCTAATACTAAGTTTGCACCTATAGTTTATCACCTGGTTTTTACCTCATCGAAATGAATCATTTATTTTTTTCCTTGTGCATCATTTCAGAGCATGTTTATAAATATACATTAATCTATCAACAATCGATTTAGAGACAACCATATTGGACATATGACAACCAATATGATAATTTGTCAACTACAAGTTCATTTTTACTCTCTTTGAAAGACAACCATATTGGACATCAAAACCGTTGACTCGAGAGACCGATCGACCTGAAATAAGATTTATCTTGAATTGGTACACATGATGAAGGCTAATTTTGCACGTTTAGTTTATCATCTGACTTAGAGACAACCATATTGGACATAAGACAACCAATATGATAACTTGTAACTACAAGTTCATTTTTACTCAAAACAGTTGTTTCGAGAAACCGACCGACCTGAAATAAGATTTATTTTGAATTGGCACACCTCATGAATGCTCATCTGATTTTTGCAGCAATATATGAAAGAAATCCTTAAAGATCTCTGTGTCTACAACAACAAGGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAGTACAAGGAAACATGTGAGGAAACGAAACCGCGATAGGTCGGTTGGTTCAATGGTTTTGGTATTTGACTGTCCATTTAGTGCAAGATTTGTGTGTTTTCCTTGGTGTTTCTTTTAGTATAGTATAGGATAGGCTTTTAGGGGATTATTTCGTATTGTCGAATTTGTCGAACTCATCAGTGTAAGCACTGG

mRNA sequence

CGGAAAATGCCTTTCTAACCAAAACCGTTTGTCCCATTTCCTTTCAATTTTTTTCACTTCCGTAGACGTATCATTGCTTTCCTTCCTCTTCTTCTTCTTGTGCTCTCTTCTGCAATGGACGGCGACGACAAACCCATCGACCGTGGCAGTTCCAGAAAACTCCACAGCAATAGCAGCACCGTCGCCGGAACCGACTTCTTGGAGACGAACAAGGCGGACAGAGCAATGTGGCTCTTGAAATGCCCGCAAGTTGTAACTCGCGCCCTTTCCAATTCGCCGGATGATCCTTCTCGTCCGGTCGCTAAGGTTATTGTTTCTGTTGATCCTCTGCAGTCGAATGACGATGATGATTCTTCATCTACTGAGTTTACTATGGAATTGGCTGACACCGACTCCGGAAAAGCATTAAGGGCCTACTCATTGAATATGTCGACAGATTTTATTCCGATGTCTGTATTTTCTGAGTCAGCACAAGGGAAGTTTACTGTTGAAGGAAAAATACTGAACAAATTTGACATGAAGCCGCATGATCAAAATCTTGATAGTTACGGGAAACTCTGTCGTGAAAGAACTCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCAATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGAGAAGAAGAAGGTGGTGAGCAAGGGATCGGAAAATAAGAGGCTGAGGAAGGAGCGTGGCGAATTGGAGAAGATCATTTTTAGGCTCTTTGAAAGACAGCCATATTGGACCTCAAAACAATTGATTCAAGAGACCGATCAACCTGAACAATATATGAAAGAAATCCTTAAAGATCTCTGTGTCTACAACAACAAGGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAGTACAAGGAAACATGTGAGGAAACGAAACCGCGATAGGTCGGTTGGTTCAATGGTTTTGGTATTTGACTGTCCATTTAGTGCAAGATTTGTGTGTTTTCCTTGGTGTTTCTTTTAGTATAGTATAGGATAGGCTTTTAGGGGATTATTTCGTATTGTCGAATTTGTCGAACTCATCAGTGTAAGCACTGG

Coding sequence (CDS)

ATGGACGGCGACGACAAACCCATCGACCGTGGCAGTTCCAGAAAACTCCACAGCAATAGCAGCACCGTCGCCGGAACCGACTTCTTGGAGACGAACAAGGCGGACAGAGCAATGTGGCTCTTGAAATGCCCGCAAGTTGTAACTCGCGCCCTTTCCAATTCGCCGGATGATCCTTCTCGTCCGGTCGCTAAGGTTATTGTTTCTGTTGATCCTCTGCAGTCGAATGACGATGATGATTCTTCATCTACTGAGTTTACTATGGAATTGGCTGACACCGACTCCGGAAAAGCATTAAGGGCCTACTCATTGAATATGTCGACAGATTTTATTCCGATGTCTGTATTTTCTGAGTCAGCACAAGGGAAGTTTACTGTTGAAGGAAAAATACTGAACAAATTTGACATGAAGCCGCATGATCAAAATCTTGATAGTTACGGGAAACTCTGTCGTGAAAGAACTCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCAATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGAGAAGAAGAAGGTGGTGAGCAAGGGATCGGAAAATAAGAGGCTGAGGAAGGAGCGTGGCGAATTGGAGAAGATCATTTTTAGGCTCTTTGAAAGACAGCCATATTGGACCTCAAAACAATTGATTCAAGAGACCGATCAACCTGAACAATATATGAAAGAAATCCTTAAAGATCTCTGTGTCTACAACAACAAGGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAGTACAAGGAAACATGTGAGGAAACGAAACCGCGATAG

Protein sequence

MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSRPVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQGKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR
Homology
BLAST of CmaCh04G025910 vs. ExPASy Swiss-Prot
Match: P41900 (General transcription factor IIF subunit 2 OS=Drosophila melanogaster OX=7227 GN=TfIIFbeta PE=2 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 6.0e-16
Identity = 72/260 (27.69%), Postives = 129/260 (49.62%), Query Frame = 0

Query: 29  LETNKADRAMWLLKCPQVVTRALSNSPDD-----------PSRPVAKVIVSVDP-LQSND 88
           L+ + A R +WL+K P+ + +    +P +           P +  A+V +S+ P + + D
Sbjct: 16  LDLSNAGRGVWLVKVPKYIAQKWEKAPTNMDVGKLRINKTPGQK-AQVSLSLTPAVLALD 75

Query: 89  DDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFS--ESAQGKFTVEGKILNKFD 148
            ++   TE  ++++   + + L  +S    +D    S  S  +    K  +EG+I+ K +
Sbjct: 76  PEEKIPTEHILDVSQV-TKQTLGVFSHMAPSDGKENSTTSAAQPDNEKLYMEGRIVQKLE 135

Query: 149 MKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 208
            +P   N   Y KL  E   K+    R++Q ID +   + +P+      +    E+KK  
Sbjct: 136 CRPIADN--CYMKLKLESIRKASEPQRRVQPIDKIV-QNFKPVKD-HAHNIEYRERKK-- 195

Query: 209 SKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 268
              +E K+ R ++  +  ++F  FE+  Y+  K L++ T+QP  Y+KEILKD+C YN K 
Sbjct: 196 ---AEGKKARDDKNAVMDMLFHAFEKHQYYNIKDLVKITNQPISYLKEILKDVCDYNMKN 255

Query: 269 VHQGTYELKPEYKETCEETK 275
            H+  +ELK EY+    E K
Sbjct: 256 PHKNMWELKKEYRHYKTEEK 264

BLAST of CmaCh04G025910 vs. ExPASy Swiss-Prot
Match: Q8R0A0 (General transcription factor IIF subunit 2 OS=Mus musculus OX=10090 GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.1e-15
Identity = 51/163 (31.29%), Postives = 90/163 (55.21%), Query Frame = 0

Query: 112 MSVFSESAQGKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTG 171
           ++VF+ES+  K ++EG ++ + + +P     ++Y KL R +  +S    R  Q +D V  
Sbjct: 93  LTVFTESSSDKLSLEGIVVQRAECRPAAS--ENYMKLKRLQIEESSKPVRLSQQLDKVVT 152

Query: 172 GHMRPMPGMDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQ 231
            + +P+       +    ++K   K  + KR R ++  +  ++F  FE+  Y+  K L+ 
Sbjct: 153 TNYKPVANH---QYNIEYERK---KKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVD 212

Query: 232 ETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKETCEETK 275
            T QP  Y+KEILK++ + N KG+H+ T+ELKPEY+    E K
Sbjct: 213 ITKQPVGYLKEILKEIGIQNVKGIHKNTWELKPEYRHYQTEEK 247

BLAST of CmaCh04G025910 vs. ExPASy Swiss-Prot
Match: P13984 (General transcription factor IIF subunit 2 OS=Homo sapiens OX=9606 GN=GTF2F2 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 1.1e-14
Identity = 51/163 (31.29%), Postives = 90/163 (55.21%), Query Frame = 0

Query: 112 MSVFSESAQGKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTG 171
           ++VF+ES+  K ++EG ++ + + +P     ++Y +L R +  +S    R  Q +D V  
Sbjct: 93  LTVFTESSSDKLSLEGIVVQRAECRPAAS--ENYMRLKRLQIEESSKPVRLSQQLDKVVT 152

Query: 172 GHMRPMPGMDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQ 231
            + +P+       +    ++K   K  + KR R ++  +  ++F  FE+  Y+  K L+ 
Sbjct: 153 TNYKPVANH---QYNIEYERK---KKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVD 212

Query: 232 ETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKETCEETK 275
            T QP  Y+KEILK++ V N KG+H+ T+ELKPEY+    E K
Sbjct: 213 ITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEK 247

BLAST of CmaCh04G025910 vs. ExPASy Swiss-Prot
Match: Q01750 (General transcription factor IIF subunit 2 OS=Rattus norvegicus OX=10116 GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.5e-14
Identity = 67/252 (26.59%), Postives = 122/252 (48.41%), Query Frame = 0

Query: 33  KADRAMWLLKCPQVVTRALSNSPDDPSRPVAKVIVSVDPLQSNDDDDSSSTEFTM--ELA 92
           K +  +WL+K P+ +++  + +P             V  L+   +   +   FT+  +LA
Sbjct: 13  KQNTGVWLVKVPKYLSQQWAKAPGRG---------EVGKLRIAKNQGRTEVSFTLNEDLA 72

Query: 93  DT-DSGKALRAYSLNMSTDFI-------PMSVFSESAQGKFTVEGKILNKFDMKPHDQNL 152
           +  D G    + S      F+        ++VF+ES+  K ++EG ++ + + +P     
Sbjct: 73  NIHDIGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAAS-- 132

Query: 153 DSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSENKR 212
           ++Y KL R +  +S    R  Q  D V   + +P+       +    ++K   K  + KR
Sbjct: 133 ENYMKLKRLQIEESSKPVRLSQQADKVVTTNYKPVANH---QYNIEYERK---KKEDGKR 192

Query: 213 LRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYEL 272
            R ++  +  ++F  FE+  Y+  K L+  T QP  Y+KEILK++ + N KG+H+ T+EL
Sbjct: 193 ARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVGYLKEILKEIGIQNVKGIHKNTWEL 247

Query: 273 KPEYKETCEETK 275
           KPEY+    E K
Sbjct: 253 KPEYRHYQTEEK 247

BLAST of CmaCh04G025910 vs. ExPASy Swiss-Prot
Match: Q2T9L9 (General transcription factor IIF subunit 2 OS=Bos taurus OX=9913 GN=GTF2F2 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.3e-14
Identity = 66/252 (26.19%), Postives = 123/252 (48.81%), Query Frame = 0

Query: 33  KADRAMWLLKCPQVVTRALSNSPDDPSRPVAKVIVSVDPLQSNDDDDSSSTEFTM--ELA 92
           K +  +WL+K P+ +++  + +P             V  L+   +   +   FT+  +LA
Sbjct: 13  KQNTGVWLVKVPKYLSQQWAKAPGRG---------EVGKLRIAKNQGRTEVSFTLNEDLA 72

Query: 93  DT-DSGKALRAYSLNMSTDFI-------PMSVFSESAQGKFTVEGKILNKFDMKPHDQNL 152
           +  D G    + S      F+        ++VF+ES+  K ++EG ++ + + +P     
Sbjct: 73  NIHDIGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAAN-- 132

Query: 153 DSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSENKR 212
           ++Y +L R +  +S    R  Q +D V   + +P+       +    ++K   K  + KR
Sbjct: 133 ENYMRLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANH---QYNIEYERK---KKEDGKR 192

Query: 213 LRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYEL 272
            R ++  +  ++F  FE+  Y+  K L+  T QP  Y+K+ILK++ V N KG+H+ T+EL
Sbjct: 193 ARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVSYLKDILKEIGVQNVKGIHKNTWEL 247

Query: 273 KPEYKETCEETK 275
           KPEY+    E K
Sbjct: 253 KPEYRHYQVEEK 247

BLAST of CmaCh04G025910 vs. ExPASy TrEMBL
Match: A0A6J1JIQ3 (Transcription initiation factor IIF subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111487292 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.2e-153
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60
           MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR
Sbjct: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60

Query: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120
           PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ
Sbjct: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120

Query: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180
           GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM
Sbjct: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180

Query: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240
           DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM
Sbjct: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240

Query: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 277
           KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR
Sbjct: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 276

BLAST of CmaCh04G025910 vs. ExPASy TrEMBL
Match: A0A6J1HK34 (Transcription initiation factor IIF subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111464830 PE=3 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 9.3e-153
Identity = 275/276 (99.64%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60
           MDGDDKPIDRGSSRKLHSNSST AGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR
Sbjct: 1   MDGDDKPIDRGSSRKLHSNSSTAAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60

Query: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120
           PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ
Sbjct: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120

Query: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180
           GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM
Sbjct: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180

Query: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240
           DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM
Sbjct: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240

Query: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 277
           KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR
Sbjct: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 276

BLAST of CmaCh04G025910 vs. ExPASy TrEMBL
Match: A0A1S3CPB8 (Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC103503304 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.2e-137
Identity = 252/277 (90.97%), Postives = 262/277 (94.58%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSST-VAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPS 60
           MD D K IDR +SRK  +N++  V GTDFLETNKADRAMWLLKCPQVVTRALSNSPD PS
Sbjct: 1   MDVDGKSIDRSTSRKHQTNNNNIVVGTDFLETNKADRAMWLLKCPQVVTRALSNSPDAPS 60

Query: 61  RPVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESA 120
           RPVAKVIVSVDPLQSNDDDDSSSTEFTMELA TDSG ALR+Y+LNMSTDFIPMSVFSES+
Sbjct: 61  RPVAKVIVSVDPLQSNDDDDSSSTEFTMELASTDSGNALRSYTLNMSTDFIPMSVFSESS 120

Query: 121 QGKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPG 180
           QGKFTVEGKILNKFDMKPHDQNL+ YGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPG
Sbjct: 121 QGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPG 180

Query: 181 MDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQY 240
           MDVLSFGAAEKKKVVSKGSE KRLRKERGELEKIIF+LFERQPYWTSKQLIQETDQPEQY
Sbjct: 181 MDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQY 240

Query: 241 MKEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 277
           MKEILKDLCVYNNKGVHQGTYELKPEYKE+ EETKPR
Sbjct: 241 MKEILKDLCVYNNKGVHQGTYELKPEYKESSEETKPR 277

BLAST of CmaCh04G025910 vs. ExPASy TrEMBL
Match: A0A6J1BZ89 (Transcription initiation factor IIF subunit beta OS=Momordica charantia OX=3673 GN=LOC111006053 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 9.3e-137
Identity = 252/278 (90.65%), Postives = 262/278 (94.24%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSSTVA--GTDFLETNKADRAMWLLKCPQVVTRALSNSPDDP 60
           MD DDKPIDR SSRKL +N+++V+  GTDFLETNKADRAMWLLKCPQ VTRALSNSPD P
Sbjct: 1   MDVDDKPIDRCSSRKLQTNNNSVSGTGTDFLETNKADRAMWLLKCPQAVTRALSNSPDGP 60

Query: 61  SRPVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSES 120
           SRPVAKVIVSVDPLQSNDDDDSSSTEFTMELA TDSG +LR+YSLNMSTDFIPMSVFSES
Sbjct: 61  SRPVAKVIVSVDPLQSNDDDDSSSTEFTMELAGTDSGNSLRSYSLNMSTDFIPMSVFSES 120

Query: 121 AQGKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMP 180
             GKFTVEGKILNKFDMKPHDQNL+ YGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMP
Sbjct: 121 PPGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMP 180

Query: 181 GMDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQ 240
           GMDV SFGAAEKKKVVSKGSE KRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQ
Sbjct: 181 GMDVTSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQ 240

Query: 241 YMKEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 277
           YMKEILKDLCVYNNKGVHQGTYELKPEYKE+ +ETK R
Sbjct: 241 YMKEILKDLCVYNNKGVHQGTYELKPEYKESGDETKSR 278

BLAST of CmaCh04G025910 vs. ExPASy TrEMBL
Match: A0A1S3CPE2 (Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC103503304 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.0e-135
Identity = 252/283 (89.05%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSST-VAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPS 60
           MD D K IDR +SRK  +N++  V GTDFLETNKADRAMWLLKCPQVVTRALSNSPD PS
Sbjct: 1   MDVDGKSIDRSTSRKHQTNNNNIVVGTDFLETNKADRAMWLLKCPQVVTRALSNSPDAPS 60

Query: 61  RPVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESA 120
           RPVAKVIVSVDPLQSNDDDDSSSTEFTMELA TDSG ALR+Y+LNMSTDFIPMSVFSES+
Sbjct: 61  RPVAKVIVSVDPLQSNDDDDSSSTEFTMELASTDSGNALRSYTLNMSTDFIPMSVFSESS 120

Query: 121 Q------GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGH 180
           Q      GKFTVEGKILNKFDMKPHDQNL+ YGKLCRERTHKSMTKSRQIQVIDHVTGGH
Sbjct: 121 QDSSFVLGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGH 180

Query: 181 MRPMPGMDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQET 240
           MRPMPGMDVLSFGAAEKKKVVSKGSE KRLRKERGELEKIIF+LFERQPYWTSKQLIQET
Sbjct: 181 MRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQET 240

Query: 241 DQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 277
           DQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKE+ EETKPR
Sbjct: 241 DQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESSEETKPR 283

BLAST of CmaCh04G025910 vs. NCBI nr
Match: XP_022990432.1 (general transcription factor IIF subunit 2-like [Cucurbita maxima] >XP_023543024.1 general transcription factor IIF subunit 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543025.1 general transcription factor IIF subunit 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 550.8 bits (1418), Expect = 6.6e-153
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60
           MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR
Sbjct: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60

Query: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120
           PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ
Sbjct: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120

Query: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180
           GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM
Sbjct: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180

Query: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240
           DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM
Sbjct: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240

Query: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 277
           KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR
Sbjct: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 276

BLAST of CmaCh04G025910 vs. NCBI nr
Match: KAG6602326.1 (General transcription factor IIF subunit 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 550.1 bits (1416), Expect = 1.1e-152
Identity = 275/276 (99.64%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60
           MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR
Sbjct: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60

Query: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120
           PVAKVIVSVDP+QSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ
Sbjct: 61  PVAKVIVSVDPMQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120

Query: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180
           GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM
Sbjct: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180

Query: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240
           DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM
Sbjct: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240

Query: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 277
           KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR
Sbjct: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 276

BLAST of CmaCh04G025910 vs. NCBI nr
Match: XP_022964851.1 (general transcription factor IIF subunit 2-like [Cucurbita moschata])

HSP 1 Score: 549.3 bits (1414), Expect = 1.9e-152
Identity = 275/276 (99.64%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60
           MDGDDKPIDRGSSRKLHSNSST AGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR
Sbjct: 1   MDGDDKPIDRGSSRKLHSNSSTAAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60

Query: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120
           PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ
Sbjct: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120

Query: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180
           GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM
Sbjct: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180

Query: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240
           DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM
Sbjct: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240

Query: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 277
           KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR
Sbjct: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 276

BLAST of CmaCh04G025910 vs. NCBI nr
Match: XP_008465665.1 (PREDICTED: general transcription factor IIF subunit 2 isoform X2 [Cucumis melo])

HSP 1 Score: 497.7 bits (1280), Expect = 6.6e-137
Identity = 252/277 (90.97%), Postives = 262/277 (94.58%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSST-VAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPS 60
           MD D K IDR +SRK  +N++  V GTDFLETNKADRAMWLLKCPQVVTRALSNSPD PS
Sbjct: 1   MDVDGKSIDRSTSRKHQTNNNNIVVGTDFLETNKADRAMWLLKCPQVVTRALSNSPDAPS 60

Query: 61  RPVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESA 120
           RPVAKVIVSVDPLQSNDDDDSSSTEFTMELA TDSG ALR+Y+LNMSTDFIPMSVFSES+
Sbjct: 61  RPVAKVIVSVDPLQSNDDDDSSSTEFTMELASTDSGNALRSYTLNMSTDFIPMSVFSESS 120

Query: 121 QGKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPG 180
           QGKFTVEGKILNKFDMKPHDQNL+ YGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPG
Sbjct: 121 QGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPG 180

Query: 181 MDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQY 240
           MDVLSFGAAEKKKVVSKGSE KRLRKERGELEKIIF+LFERQPYWTSKQLIQETDQPEQY
Sbjct: 181 MDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQY 240

Query: 241 MKEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 277
           MKEILKDLCVYNNKGVHQGTYELKPEYKE+ EETKPR
Sbjct: 241 MKEILKDLCVYNNKGVHQGTYELKPEYKESSEETKPR 277

BLAST of CmaCh04G025910 vs. NCBI nr
Match: XP_038889594.1 (general transcription factor IIF subunit 2-like [Benincasa hispida] >XP_038889595.1 general transcription factor IIF subunit 2-like [Benincasa hispida] >XP_038889596.1 general transcription factor IIF subunit 2-like [Benincasa hispida])

HSP 1 Score: 497.3 bits (1279), Expect = 8.6e-137
Identity = 252/275 (91.64%), Postives = 259/275 (94.18%), Query Frame = 0

Query: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60
           MD D K IDR SSRKL  N+++V GTDFLETNKADRAMWLLKCPQVVTRALSNSPD PSR
Sbjct: 1   MDVDGKSIDRCSSRKLQINNNSVVGTDFLETNKADRAMWLLKCPQVVTRALSNSPDGPSR 60

Query: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ 120
           PVAKVIVSVDPLQSNDDDDSSSTEFTMELA TDSG  LR+YSLNMSTDFIPMSVFSES+Q
Sbjct: 61  PVAKVIVSVDPLQSNDDDDSSSTEFTMELAGTDSGNTLRSYSLNMSTDFIPMSVFSESSQ 120

Query: 121 GKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180
           GKFTVEGKILNKFDMKPHDQNL+ YGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM
Sbjct: 121 GKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM 180

Query: 181 DVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYM 240
           DVLSFGAAEKKKVVSKGSE KRLRKERGELEKIIFRLFERQ YWTSKQLIQETDQPEQYM
Sbjct: 181 DVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQSYWTSKQLIQETDQPEQYM 240

Query: 241 KEILKDLCVYNNKGVHQGTYELKPEYKETCEETKP 276
           KEILKDLCVYNNKGVHQGTYELKPEYKE+ EE KP
Sbjct: 241 KEILKDLCVYNNKGVHQGTYELKPEYKESGEEAKP 275

BLAST of CmaCh04G025910 vs. TAIR 10
Match: AT3G52270.1 (Transcription initiation factor IIF, beta subunit )

HSP 1 Score: 285.8 bits (730), Expect = 3.7e-77
Identity = 146/252 (57.94%), Postives = 189/252 (75.00%), Query Frame = 0

Query: 29  LETNKADRAMWLLKCPQVVTRALSN--------SPDDPSRPVAKVIVSVDPLQSNDDDDS 88
           LET  A+R+M L+K P +V  +L +         PDDP RP AKVI+ VDPL   D+   
Sbjct: 23  LETGLAERSMLLMKAPSLVASSLQSHSFPDDPYRPDDPYRPDAKVILGVDPLAHEDE--- 82

Query: 89  SSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQGKFTVEGKILNKFDMKPHDQ 148
             T+F MELA  DSG   R Y+L+MS DFIPM+VF ES+ GK +VEGKI NKFDM+PH++
Sbjct: 83  -GTQFVMELARADSGNMPRRYTLDMSKDFIPMNVFCESSDGKMSVEGKIKNKFDMRPHNE 142

Query: 149 NLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSEN 208
           N++SYG+LCRERT+K M K+RQIQVID+  G HMRPMPGM ++   A EKKK+ ++ SE 
Sbjct: 143 NIESYGRLCRERTNKYMGKNRQIQVIDNARGMHMRPMPGM-IIPTAAPEKKKLTNRTSEM 202

Query: 209 KRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTY 268
           KR R++R E+E+++F LFERQ  WT + LIQETDQPEQ++K++LKDLC+YNNKG +QGTY
Sbjct: 203 KRTRRDRREMEEVMFNLFERQSNWTLRLLIQETDQPEQFLKDLLKDLCIYNNKGSNQGTY 262

Query: 269 ELKPEYKETCEE 273
           ELKPEYK+  +E
Sbjct: 263 ELKPEYKKATQE 269

BLAST of CmaCh04G025910 vs. TAIR 10
Match: AT1G75510.1 (Transcription initiation factor IIF, beta subunit )

HSP 1 Score: 242.3 bits (617), Expect = 4.7e-64
Identity = 129/255 (50.59%), Postives = 174/255 (68.24%), Query Frame = 0

Query: 29  LETNKADRAMWLLKCPQVVTRA---------LSNSPDDPSRPVAKVIVSVDPLQSNDDDD 88
           L+  K+DR++WL+KCP VV +A          S +  D    +AK++  VDPL+     D
Sbjct: 7   LDIEKSDRSIWLMKCPVVVDKAWHKIAASSSSSFASSDSPPDMAKIVREVDPLR-----D 66

Query: 89  SSSTEFTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQGKFTVEGKILNKFDMKPHD 148
            S  EF M +   + G   + Y+LNM TDF+PM  FS+  QG    EGK+ +KFDMKP+ 
Sbjct: 67  DSPPEFKMYMVGAEYGNMPKCYALNMFTDFVPMGGFSDVNQGCAAAEGKVDHKFDMKPYG 126

Query: 149 QNLDSYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM-DVLSFGAAEKKKVVS-KG 208
           + ++ Y +LCRERT K+M K+RQIQVID+  G HMRPMPGM  ++S  + EK+K    K 
Sbjct: 127 ETIEEYARLCRERTSKAMVKNRQIQVIDNDRGVHMRPMPGMLGLVSSNSKEKRKPPPVKQ 186

Query: 209 SENKRLRKERGELEKIIFRLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQ 268
           +E KR R++RGELE I+F+LFE QP WT KQL+QETDQP Q++KEIL +LCVYN +G +Q
Sbjct: 187 TEVKRTRRDRGELEAIMFKLFEGQPNWTLKQLVQETDQPAQFLKEILNELCVYNKRGSNQ 246

Query: 269 GTYELKPEYKETCEE 273
           GTYELKPEYK++ E+
Sbjct: 247 GTYELKPEYKKSAED 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P419006.0e-1627.69General transcription factor IIF subunit 2 OS=Drosophila melanogaster OX=7227 GN... [more]
Q8R0A05.1e-1531.29General transcription factor IIF subunit 2 OS=Mus musculus OX=10090 GN=Gtf2f2 PE... [more]
P139841.1e-1431.29General transcription factor IIF subunit 2 OS=Homo sapiens OX=9606 GN=GTF2F2 PE=... [more]
Q017502.5e-1426.59General transcription factor IIF subunit 2 OS=Rattus norvegicus OX=10116 GN=Gtf2... [more]
Q2T9L93.3e-1426.19General transcription factor IIF subunit 2 OS=Bos taurus OX=9913 GN=GTF2F2 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JIQ33.2e-153100.00Transcription initiation factor IIF subunit beta OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1HK349.3e-15399.64Transcription initiation factor IIF subunit beta OS=Cucurbita moschata OX=3662 G... [more]
A0A1S3CPB83.2e-13790.97Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1BZ899.3e-13790.65Transcription initiation factor IIF subunit beta OS=Momordica charantia OX=3673 ... [more]
A0A1S3CPE23.0e-13589.05Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_022990432.16.6e-153100.00general transcription factor IIF subunit 2-like [Cucurbita maxima] >XP_023543024... [more]
KAG6602326.11.1e-15299.64General transcription factor IIF subunit 2, partial [Cucurbita argyrosperma subs... [more]
XP_022964851.11.9e-15299.64general transcription factor IIF subunit 2-like [Cucurbita moschata][more]
XP_008465665.16.6e-13790.97PREDICTED: general transcription factor IIF subunit 2 isoform X2 [Cucumis melo][more]
XP_038889594.18.6e-13791.64general transcription factor IIF subunit 2-like [Benincasa hispida] >XP_03888959... [more]
Match NameE-valueIdentityDescription
AT3G52270.13.7e-7757.94Transcription initiation factor IIF, beta subunit [more]
AT1G75510.14.7e-6450.59Transcription initiation factor IIF, beta subunit [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 202..268
e-value: 8.1E-31
score: 107.9
IPR040450TFIIF beta subunit, HTH domainPFAMPF02270TFIIF_betacoord: 204..267
e-value: 8.4E-22
score: 77.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR10445:SF2TRANSCRIPTION INITIATION FACTOR IIF, BETA SUBUNITcoord: 22..273
IPR003196Transcription initiation factor IIF, beta subunitPANTHERPTHR10445GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2coord: 22..273
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 203..268
IPR011039Transcription Factor IIF, Rap30/Rap74, interactionSUPERFAMILY50916Rap30/74 interaction domainscoord: 29..138

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G025910.1CmaCh04G025910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0006366 transcription by RNA polymerase II
cellular_component GO:0005674 transcription factor TFIIF complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0008168 methyltransferase activity