CmaCh04G024030 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G024030
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionwall-associated receptor kinase 3-like
LocationCma_Chr04: 16611088 .. 16613213 (-)
RNA-Seq ExpressionCmaCh04G024030
SyntenyCmaCh04G024030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACCCATCATGGCATTCATGGTGATGATGATCATAGCCATATTATCAACACCCTTGGCCGTGGCAGCATCTCAAGCCTTACCCGGGTGTGAAGAACGGTGTGGCGACGTGCACATTCCATATCCATTCGGCATCAAAGGGTGCTATTTCAATCAAAATTCCTCGGTCAATTTCTCGGTCACTTGCCACAAAACTGATGGCAACCGACCTCTAAAGGCGTTTCTAATGCATACCCATATTAGGATTATCAACCTATCTGCTAACGGTGAGCTTCATGCATTGGGGAGGGTAGTGCAAAATTGTTACAGGGACGCATTTACGAGGGTTTCCAATCTTTCTGTGCCGGACATGTACCCACTTTCTCGTTCCAAAAATAAGTTCATTACCCTTGGGTGCAATCATGTTGGGTTAATCATGGGGGATTACTTAGGGGGTGACTTTGAAAGTGGGTGTATTTCTGGGAGTGGGTGCTGTCAGTTGGAGATTCCAGATGGGTTAAGAAATTTGAGATTGGCGGTGGGTGAGTTGTTGAATTCCACTGACGTATGGAACTTCAATCCCTGTTGGTATGCTTTTATAATCGAAGCTGAGAGGTTCAAGTTTTTGTCAAGTTTTATTAAGAATTTTAAAGATAAGGAGGTTGAGGTTGTGCTTGGTTGGGGCATTAGAAACGAATTAGAACTTGATTGTGGACTAAACGCCCGAAGGAATAATAGTATCTTTAATGGAACTCATTATCGTTGCCAATGCTTGGATGGTTACGGGGGAATCCATATCTCACTCAAGGATGTCAAGGTAAAGGCTTATTTATCTTTCGATTGGATGGTGAGATCTCATATGGATTAAAAAGGAAAACATAACATTTCTTATAAACATGTGGAATCTCTCTCTAGAAGACGCGTTTTAAAACTGTGAGGCTGACAGCTATACATAACAGGCCAAAACGGACAATATCTGCTAGCGGTAGGCTTGAGCTGTTACAAATAGTATCAGAGTTAAATGTTGATCCCCCAAGGGGGTGGATTGTGAGATCTCACATCCGTTGTAGAGGGGAACAAAGCATTTAAGGGTGTGGAAACCTCTACTAGCAAACGAGTTTTAAAACCGTGAGTTTGACGGCGATGCGTAATAGGCCAAAACAACAATATTGGTGGACTTGGGTAATTACAAATGGTATTAGAGCTAGACACCAGACAGTGTGCTAGCGAGGACGCTAGCCCCCCTAGTAGGTGGATTGTGTGATCCCATATCGGTTGGAGAGAAGAACGAAGTATTCCTTATAAGGGCGTGGAAACCTCTCCCTAGAAAACTCGTTTTAAAATCGTGAGGTTGACGGCGATACATAACGAGATAAAACGGATAATATCGGTGGACTTGAGATGTTACAAATGGTATCAGAGCTAGACACCAAATGGTGTGTCAGTGAGGACGCTAGCTCCCAAGGGGTGAATTGTAAAACCCTACCTCGATGGGAGGAAACTAAACCTTTTTTATAAATGTGTGAAAATTTTTCTCAAGCACATATCAAAATCAACTGTTTGATGATTTTAAAATAAGTTTTATTAGGATGGGTAACCTTAACCCTAAATTTGGAACCCTTTTTAGATGTGGATGAATGCAAGTATGCACGGCTAAATGACTGTATGCACAAGGCCAAGTGTATTAACACTGAAGGAAACTATACCTGTCGTTGTCCTAAGAACTTCCATGGAGATGGAAGAAAAAGGGGAGAAGGTTGCAACCCAGATTCCACCTCTTCCATTCCTATCATCATTGGTAAGTTTTTGTATGTTCTTCACCCATGTTTTTCACTATAATATACTAAACAAACATACAAATAACATGCAGGAATTGGGTTAGGCCTCGCAGTTCTACTAATTGCCACCACAACCATATACTTATGCTACAAGAAGTTGAAGTTCATCAAACAAAAACAGCGTTTTTTCCACAAAAACGGAGCCTTTGTGCTTCAACGACAGCTTTCTCAATGCAATTCACCTAAAGACATCGTAAGAATCTTCAGCCAACAAGAGTTGGAGAAGGCCACCAACAACTACGCCCAAGACACCATTGCTGGCAAAGGTGGCTACGGTACCGTTTACAAAGGTGTCTTGGAGGATTGA

mRNA sequence

ATGAAACCCATCATGGCATTCATGGTGATGATGATCATAGCCATATTATCAACACCCTTGGCCGTGGCAGCATCTCAAGCCTTACCCGGGTGTGAAGAACGGTGTGGCGACGTGCACATTCCATATCCATTCGGCATCAAAGGGTGCTATTTCAATCAAAATTCCTCGGTCAATTTCTCGGTCACTTGCCACAAAACTGATGGCAACCGACCTCTAAAGGCGTTTCTAATGCATACCCATATTAGGATTATCAACCTATCTGCTAACGGTGAGCTTCATGCATTGGGGAGGGTAGTGCAAAATTGTTACAGGGACGCATTTACGAGGGTTTCCAATCTTTCTGTGCCGGACATGTACCCACTTTCTCGTTCCAAAAATAAGTTCATTACCCTTGGGTGCAATCATGTTGGGTTAATCATGGGGGATTACTTAGGGGGTGACTTTGAAAGTGGGTGTATTTCTGGGAGTGGGTGCTGTCAGTTGGAGATTCCAGATGGGTTAAGAAATTTGAGATTGGCGGTGGGTGAGTTGTTGAATTCCACTGACGTATGGAACTTCAATCCCTGTTGGTATGCTTTTATAATCGAAGCTGAGAGGTTCAAGTTTTTGTCAAGTTTTATTAAGAATTTTAAAGATAAGGAGGTTGAGGTTGTGCTTGGTTGGGGCATTAGAAACGAATTAGAACTTGATTGTGGACTAAACGCCCGAAGGAATAATAGTATCTTTAATGGAACTCATTATCGTTGCCAATGCTTGGATGGTTACGGGGGAATCCATATCTCACTCAAGGATGTCAAGAAGACGCGTTTTAAAACTGTGAGGCTGACAGCTATACATAACAGGCCAAAACGGACAATATCTGCTAGCGATGTGGATGAATGCAAGTATGCACGGCTAAATGACTGTATGCACAAGGCCAAGTGTATTAACACTGAAGGAAACTATACCTGTCGTTGTCCTAAGAACTTCCATGGAGATGGAAGAAAAAGGGGAGAAGGTTGCAACCCAGATTCCACCTCTTCCATTCCTATCATCATTGGAATTGGGTTAGGCCTCGCAGTTCTACTAATTGCCACCACAACCATATACTTATGCTACAAGAAGTTGAAGTTCATCAAACAAAAACAGCGTTTTTTCCACAAAAACGGAGCCTTTGTGCTTCAACGACAGCTTTCTCAATGCAATTCACCTAAAGACATCGTAAGAATCTTCAGCCAACAAGAGTTGGAGAAGGCCACCAACAACTACGCCCAAGACACCATTGCTGGCAAAGGTGGCTACGGTACCGTTTACAAAGGTGTCTTGGAGGATTGA

Coding sequence (CDS)

ATGAAACCCATCATGGCATTCATGGTGATGATGATCATAGCCATATTATCAACACCCTTGGCCGTGGCAGCATCTCAAGCCTTACCCGGGTGTGAAGAACGGTGTGGCGACGTGCACATTCCATATCCATTCGGCATCAAAGGGTGCTATTTCAATCAAAATTCCTCGGTCAATTTCTCGGTCACTTGCCACAAAACTGATGGCAACCGACCTCTAAAGGCGTTTCTAATGCATACCCATATTAGGATTATCAACCTATCTGCTAACGGTGAGCTTCATGCATTGGGGAGGGTAGTGCAAAATTGTTACAGGGACGCATTTACGAGGGTTTCCAATCTTTCTGTGCCGGACATGTACCCACTTTCTCGTTCCAAAAATAAGTTCATTACCCTTGGGTGCAATCATGTTGGGTTAATCATGGGGGATTACTTAGGGGGTGACTTTGAAAGTGGGTGTATTTCTGGGAGTGGGTGCTGTCAGTTGGAGATTCCAGATGGGTTAAGAAATTTGAGATTGGCGGTGGGTGAGTTGTTGAATTCCACTGACGTATGGAACTTCAATCCCTGTTGGTATGCTTTTATAATCGAAGCTGAGAGGTTCAAGTTTTTGTCAAGTTTTATTAAGAATTTTAAAGATAAGGAGGTTGAGGTTGTGCTTGGTTGGGGCATTAGAAACGAATTAGAACTTGATTGTGGACTAAACGCCCGAAGGAATAATAGTATCTTTAATGGAACTCATTATCGTTGCCAATGCTTGGATGGTTACGGGGGAATCCATATCTCACTCAAGGATGTCAAGAAGACGCGTTTTAAAACTGTGAGGCTGACAGCTATACATAACAGGCCAAAACGGACAATATCTGCTAGCGATGTGGATGAATGCAAGTATGCACGGCTAAATGACTGTATGCACAAGGCCAAGTGTATTAACACTGAAGGAAACTATACCTGTCGTTGTCCTAAGAACTTCCATGGAGATGGAAGAAAAAGGGGAGAAGGTTGCAACCCAGATTCCACCTCTTCCATTCCTATCATCATTGGAATTGGGTTAGGCCTCGCAGTTCTACTAATTGCCACCACAACCATATACTTATGCTACAAGAAGTTGAAGTTCATCAAACAAAAACAGCGTTTTTTCCACAAAAACGGAGCCTTTGTGCTTCAACGACAGCTTTCTCAATGCAATTCACCTAAAGACATCGTAAGAATCTTCAGCCAACAAGAGTTGGAGAAGGCCACCAACAACTACGCCCAAGACACCATTGCTGGCAAAGGTGGCTACGGTACCGTTTACAAAGGTGTCTTGGAGGATTGA

Protein sequence

MKPIMAFMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIKGCYFNQNSSVNFSVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDAFTRVSNLSVPDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCISGSGCCQLEIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVVLGWGIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNRPKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIVRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED
Homology
BLAST of CmaCh04G024030 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 173.7 bits (439), Expect = 4.5e-42
Identity = 135/465 (29.03%), Postives = 210/465 (45.16%), Query Frame = 0

Query: 9   VMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNFSVTCHKTD 68
           +++I  +  T L     Q    C+ +CG+V I YPFGI  GCY+  +   NF++TC   +
Sbjct: 9   LVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDD--NFNLTCVVEE 68

Query: 69  GNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCY---RDAFTRVSNLSVPDMYPLSRS 128
                   L+   I++ N+S +G +  L      CY    +         +   + LS S
Sbjct: 69  ------KLLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETNGTALGYQLGSSFSLS-S 128

Query: 129 KNKFITLGCNHVGLIMGDYLGGDFESGCIS-------------GSGCCQLE--------- 188
            NKF  +GCN + L +  +   ++ +GC+S             G GCC  E         
Sbjct: 129 NNKFTLVGCNALSL-LSTFGKQNYSTGCLSLCNSQPEANGRCNGVGCCTTEDFSVPFDSD 188

Query: 189 -IPDGLRNLRLAVGELLN--STDVWNFNPCWYAFIIEAERFKFLSS-FIKNFKD-KEVEV 248
               G   LR  V   L+  +T V+ FNPC YAF++E  +F F SS  +KN ++     V
Sbjct: 189 TFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTRFPV 248

Query: 249 VLGWGIRNELELDCGLN--ARRNNSIFNGT---HYRCQCLDGYGGIHISLKDVKKTRFKT 308
            L W I N+     G      +N+S +N T    Y C+C +GY G               
Sbjct: 249 ALDWSIGNQTCEQAGSTRICGKNSSCYNSTTRNGYICKCNEGYDG--------------- 308

Query: 309 VRLTAIHNRPKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGE 368
                    P R+    D+DEC  +  ++C     C N +G + C+CP    G       
Sbjct: 309 --------NPYRSEGCKDIDEC-ISDTHNCSDPKTCRNRDGGFDCKCPS---GYDLNSSM 368

Query: 369 GCNPDSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLS 428
            C         I + I +G+ VLL+A   I    K+ K+ K +++FF +NG  +L ++LS
Sbjct: 369 SCTRPEYKRTRIFLVIIIGVLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLS 428

Query: 429 QCNSPKDIVRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
                    +IF+++ +++ATN Y +  I G+GG GTVYKG+L D
Sbjct: 429 GAGLSNIDFKIFTEEGMKEATNGYDESRILGQGGQGTVYKGILPD 436

BLAST of CmaCh04G024030 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.3e-41
Identity = 124/435 (28.51%), Postives = 205/435 (47.13%), Query Frame = 0

Query: 31  CEERCGDVHIPYPFGIK-GCYFNQNSSVNFSVTCHKTDGNRPLKAFLMHTHIRIINLSAN 90
           C+ RCG+V + YPFG   GCY+  + S  F++TC++ +        L   ++ +IN+S +
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDES--FNLTCNEQEK-------LFFGNMPVINMSLS 88

Query: 91  GELHALGRVVQN--CYRDAFTR---VSNLSVPDMYPLSRSKNKFITLGCNHVGLIMGDYL 150
           G+L    R+V++  CY     +   ++  +    + LS   N+F  +GCN    +    +
Sbjct: 89  GQLRV--RLVRSRVCYDSQGKQTDYIAQRTTLGNFTLS-ELNRFTVVGCNSYAFLRTSGV 148

Query: 151 GGDFESGCI-------------SGSGCCQLEIPDGLRNLRLAVGELLNSTDVWNFNPCWY 210
              + +GCI             SG GCCQ+ +P G   +R+      N   V  FNPC Y
Sbjct: 149 -EKYSTGCISICDSATTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTY 208

Query: 211 AFIIEAERFKFLS-SFIKNFKD-KEVEVVLGWGIRN------ELELDCGLNARRNNSIFN 270
           AF++E   F F +   + N ++     VVL W I +      E    CG N+   +S   
Sbjct: 209 AFLVEDGMFDFHALEDLNNLRNVTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDST-G 268

Query: 271 GTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNRPKRTISASDVDECKYARLNDCM 330
           GT Y C+CL+G+ G                        P       D++EC  +R N C 
Sbjct: 269 GTGYNCKCLEGFEG-----------------------NPYLPNGCQDINECISSRHN-CS 328

Query: 331 HKAKCINTEGNYTCRCPKNFHGDG-RKRGEGCNPDSTSSIPIIIGIGLGLAVLLIATTTI 390
             + C NT+G++ C CP  +  D          P+      I +G  +G +V+++  + +
Sbjct: 329 EHSTCENTKGSFNCNCPSGYRKDSLNSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCL 388

Query: 391 YLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIVRIFSQQELEKATNNYAQDTIA 438
               K  K  + +Q+FF +NG  +L +++S        V+IF+++ +++ATN Y +  I 
Sbjct: 389 QQKIKHRKNTELRQKFFEQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRIL 425

BLAST of CmaCh04G024030 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 5.6e-40
Identity = 126/459 (27.45%), Postives = 209/459 (45.53%), Query Frame = 0

Query: 7   FMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGI-KGCYFNQNSSVNFSVTCHK 66
           F+V +  ++  T L     Q    C+ +CG++ I YPFGI  GCY+  N S  FS+TC +
Sbjct: 8   FLVAIFFSLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNES--FSITCKE 67

Query: 67  TDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDAFTRVSNLSVPDMYPLSRS- 126
              +RP     + + I + N + +G+L  L      CY +   +    S   +  LS S 
Sbjct: 68  ---DRP----HVLSDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSA 127

Query: 127 KNKFITLGCNHVGLIMGDYLG-GDFESGCIS-------------GSGCCQLEI--PDGLR 186
            NK   +GCN + L+  D  G  ++ + C+S             G GCC++++  P    
Sbjct: 128 NNKLTAVGCNALSLL--DTFGMQNYSTACLSLCDSPPEADGECNGRGCCRVDVSAPLDSY 187

Query: 187 NLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSS-FIKNFKD-KEVEVVLGWGIRNE 246
                 G + + T   +F+PC YAF++E ++F F S+  + N ++     V+L W + N+
Sbjct: 188 TFETTSGRIKHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQ 247

Query: 247 LELDCGLNA--RRNNSIFNGT---HYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNR 306
                G  +    N++  + T    Y C+C +G+ G                        
Sbjct: 248 TCEQVGSTSICGGNSTCLDSTPRNGYICRCNEGFDG-----------------------N 307

Query: 307 PKRTISASDVDECKYA---RLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDS 366
           P  +    DV+EC  +     ++C     C N  G + C+C   +  D       C    
Sbjct: 308 PYLSAGCQDVNECTTSSTIHRHNCSDPKTCRNKVGGFYCKCQSGYRLDTTTM--SCKRKE 367

Query: 367 TSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPK 426
            +   I++   +G  V+L+    I    K LK  K +++FF +NG  +L ++LS      
Sbjct: 368 FAWTTILLVTTIGFLVILLGVACIQQRMKHLKDTKLREQFFEQNGGGMLTQRLSGAGPSN 427

Query: 427 DIVRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
             V+IF++  ++KATN YA+  I G+GG GTVYKG+L D
Sbjct: 428 VDVKIFTEDGMKKATNGYAESRILGQGGQGTVYKGILPD 430

BLAST of CmaCh04G024030 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 5.6e-40
Identity = 133/476 (27.94%), Postives = 214/476 (44.96%), Query Frame = 0

Query: 1   MKPIMAFMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNF 60
           MK    F++ +   +  T L  A  Q    C+ RCGDV I YPFGI  GCY+  + S  F
Sbjct: 1   MKVHSLFLMAIFFYLAYTQLVKA--QPRDDCQTRCGDVPIDYPFGISTGCYYPGDDS--F 60

Query: 61  SVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHAL--------GRVVQNCYRDAFTRVS 120
           ++TC +   N       + ++I ++N + +G+L  L         +   N +   + R+ 
Sbjct: 61  NITCEEDKPN-------VLSNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLD 120

Query: 121 NLSVPDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCIS-------------GSGC 180
           NLS           NKF  +GCN    ++  +   ++ +GC+S             G GC
Sbjct: 121 NLSF-------SPNNKFTLVGCN-AWALLSTFGIQNYSTGCMSLCDTPPPPNSKCNGVGC 180

Query: 181 CQLEIPDGLRNLRLAV--GELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKD---- 240
           C+ E+   L + R+        N T V +FNPC YAF +E   F F  S +++ KD    
Sbjct: 181 CRTEVSIPLDSHRIETQPSRFENMTSVEHFNPCSYAFFVEDGMFNF--SSLEDLKDLRNV 240

Query: 241 KEVEVVLGWGIRNE-LELDCGLNARRNNSI----FNGTHYRCQCLDGYGGIHISLKDVKK 300
               V+L W I N+  E   G N    NS       G  Y C+CL G+ G          
Sbjct: 241 TRFPVLLDWSIGNQTCEQVVGRNICGGNSTCFDSTRGKGYNCKCLQGFDG---------- 300

Query: 301 TRFKTVRLTAIHNRPKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDG 360
                         P  +    D++EC   R+++C   + C NT G++ C+CP     D 
Sbjct: 301 -------------NPYLSDGCQDINECT-TRIHNCSDTSTCENTLGSFHCQCPSG--SDL 360

Query: 361 RKRGEGC------NPDSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHK 420
                 C       P       +++G  +G  ++L+  + I    +  K  + +Q+FF +
Sbjct: 361 NTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHRKNTELRQQFFEQ 420

Query: 421 NGAFVLQRQLSQCNSPKDIVRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
           NG  +L ++LS        V+IF+++ +++AT+ Y +  I G+GG GTVYKG+L+D
Sbjct: 421 NGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVYKGILQD 429

BLAST of CmaCh04G024030 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.3e-36
Identity = 125/455 (27.47%), Postives = 203/455 (44.62%), Query Frame = 0

Query: 20  LAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNFSVTCHKTDGNRPLKAFLMH 79
           + +   Q LP C E+CG+V + YPFG   GC+  ++ S N S           +   L +
Sbjct: 17  MQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSC----------VNENLFY 76

Query: 80  THIRIINLSANGELHALGRVVQNCY--RDAFTRVSNLSVPDMYPLSRSKNKFIT-LGCNH 139
             + ++ +S + +L  L      CY  +  F +       ++  L+ S N  IT LGCN 
Sbjct: 77  KGLEVVEISHSSQLRVLYPASYICYNSKGKFAK-GTYYWSNLGNLTLSGNNTITALGCNS 136

Query: 140 VGLIMGDYLGGDFESGCIS--------------GSGCCQLEIPDGLRNLRLAVGELLNST 199
              +  +    +   GCIS              G GCCQ  +P G   L +      N T
Sbjct: 137 YAFVSSNGTRRN-SVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLIVRSYRFDNDT 196

Query: 200 DVWNFN--PCWYAFIIEAERFKFLS----SFIKNFKDKEVEVVLGWGIRNEL-----ELD 259
            V   +   C YAF++E  +FK+ +    S+++N ++    VVL W IR E      E  
Sbjct: 197 SVQPISEGQCIYAFLVENGKFKYNASDKYSYLQN-RNVGFPVVLDWSIRGETCGQVGEKK 256

Query: 260 CGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNRPKRTISASD 319
           CG+N   +NS  +G  Y C+C  G+ G                        P       D
Sbjct: 257 CGVNGICSNSA-SGIGYTCKCKGGFQG-----------------------NPYLQNGCQD 316

Query: 320 VDECKYA---RLNDCMHKAKCINTEGNYTCRCPKNFH-----GDGRKRGEGCNPDSTSSI 379
           ++EC  A     ++C   + C N  G++ C C   +         + +G   NP+     
Sbjct: 317 INECTTANPIHKHNCSGDSTCENKLGHFRCNCRSRYELNTTTNTCKPKG---NPEYVEWT 376

Query: 380 PIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIVR 438
            I++G  +G  V+L+A + I    K  K  + +Q+FF +NG  +L ++LS        V+
Sbjct: 377 TIVLGTTIGFLVILLAISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVK 431

BLAST of CmaCh04G024030 vs. ExPASy TrEMBL
Match: A0A6J1H8D6 (uncharacterized protein LOC111460588 OS=Cucurbita moschata OX=3662 GN=LOC111460588 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 7.5e-181
Identity = 329/456 (72.15%), Postives = 356/456 (78.07%), Query Frame = 0

Query: 1    MKPIMAFMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNF 60
            MKPIMAF+VMM IAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK GCY NQ    NF
Sbjct: 1098 MKPIMAFLVMMNIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIKEGCYLNQ----NF 1157

Query: 61   SVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDAFTRV----SNLSV 120
            S+ C KTD N P KAFLMHT+I ++N+  NGELH L  +V+NCY D  + +    S LSV
Sbjct: 1158 SIACDKTDRNGPPKAFLMHTNISVMNIFTNGELHVLQPIVRNCYSDGGSYLDMNDSYLSV 1217

Query: 121  PDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCI--------------SGSGCCQL 180
            PDMYPLSRSKNKFIT+GCNHVGLI+GDYLGGDFESGCI              SGSGCCQL
Sbjct: 1218 PDMYPLSRSKNKFITIGCNHVGLILGDYLGGDFESGCISMCTKKSSVADGSCSGSGCCQL 1277

Query: 181  EIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVVLGW 240
            EIP GL NLRLAVGELLN T++   +PC YAFIIEAERF+FLSS+I  F+D++V VVL W
Sbjct: 1278 EIPKGLTNLRLAVGELLNYTEIPKLSPCGYAFIIEAERFRFLSSYIDKFEDEKVVVVLSW 1337

Query: 241  GIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNR 300
            GIRNEL+ +CG N  R  SIFNGT Y CQCLDGY G                        
Sbjct: 1338 GIRNELKFECGSNTAR-ISIFNGTQYGCQCLDGYEG-----------------------N 1397

Query: 301  PKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDSTSS 360
            P       DV+ECKY  LNDCMHKAKCINTEGNYTCRCPKNFHGDGRK GEGC  +STSS
Sbjct: 1398 PYLRHGCQDVNECKYGWLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKGGEGCTKNSTSS 1457

Query: 361  IPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIV 420
            IPIIIGIGLGL VLLIA TTIYLCYKKLKFIKQKQRFFHKNG FVLQRQLSQCNSPKDIV
Sbjct: 1458 IPIIIGIGLGLVVLLIAITTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIV 1517

Query: 421  RIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
            RIFSQ+ELEKATNNYA+DTIAGKGGYGTVYKGVL+D
Sbjct: 1518 RIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDD 1525

BLAST of CmaCh04G024030 vs. ExPASy TrEMBL
Match: A0A6J1JGR1 (wall-associated receptor kinase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111486860 PE=4 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 6.3e-172
Identity = 320/459 (69.72%), Postives = 352/459 (76.69%), Query Frame = 0

Query: 1   MKPIMAFMVMMI-IAILSTPLAVAASQALPGCEERCGDVHIPYPFGI-KGCYFNQNSSVN 60
           +KPIMAFMVMM+ I ILST LAV AS+ALPGC+E CG VHIPYPFGI KGCY NQ    N
Sbjct: 5   IKPIMAFMVMMMKIGILSTALAVEASEALPGCDEWCGKVHIPYPFGIKKGCYLNQ----N 64

Query: 61  FSVTCHKTDGNRPLK--AFLMHTHIRIINLSANGELHALGRVVQNCYR--DAFTRV--SN 120
           FS+TCHKTD N P K  AFLMHT+IR++N+S NGELH L  +V+NCY   D +  +  ++
Sbjct: 65  FSITCHKTDLNGPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETD 124

Query: 121 LSVPDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCI--------------SGSGC 180
           LSVP MYPLSRSKNKFIT+GCNHVGLI+GD  G + ESGCI              SGSGC
Sbjct: 125 LSVPTMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRISVVDGLCSGSGC 184

Query: 181 CQLEIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVV 240
           CQLEIP GL  L LAVGELLN T++  F+PC YAFIIEA RF+FLSS+I  F++KEVEVV
Sbjct: 185 CQLEIPKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEVV 244

Query: 241 LGWGIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAI 300
           L WGIR ELELDCGLNARRN+SIFNGTHYRCQCLDGY G                     
Sbjct: 245 LSWGIRKELELDCGLNARRNSSIFNGTHYRCQCLDGYEG--------------------- 304

Query: 301 HNRPKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDS 360
              P       DVDECKY  LNDC HK KC NTEGNYTC CPKNFHGDGRK G+GC  +S
Sbjct: 305 --NPYLRHGCQDVDECKYPWLNDCKHKDKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNS 364

Query: 361 TSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPK 420
           TSSIPIIIGIG+GL VLLIATTTIYL YKKLKFIKQKQ FF KNG FVLQRQLSQCNSPK
Sbjct: 365 TSSIPIIIGIGVGLPVLLIATTTIYLGYKKLKFIKQKQLFFQKNGGFVLQRQLSQCNSPK 424

Query: 421 DIVRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
           DIVRIFSQ+ELEKATNNYAQDTIAGKGGYGTV+KGVL+D
Sbjct: 425 DIVRIFSQEELEKATNNYAQDTIAGKGGYGTVFKGVLDD 436

BLAST of CmaCh04G024030 vs. ExPASy TrEMBL
Match: A0A6J1H6B0 (uncharacterized protein LOC111460594 OS=Cucurbita moschata OX=3662 GN=LOC111460594 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.9e-168
Identity = 311/457 (68.05%), Postives = 345/457 (75.49%), Query Frame = 0

Query: 1    MKPIMAFMVMMI-IAILSTPLAVAASQALPGCEERCGDVHIPYPFGI-KGCYFNQNSSVN 60
            +KPIMAFMVMM+ IAILST LAVAASQALPGC+E CG VHIPYPFGI KGCY NQ    N
Sbjct: 999  IKPIMAFMVMMMKIAILSTALAVAASQALPGCDEWCGKVHIPYPFGIKKGCYLNQ----N 1058

Query: 61   FSVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDAFTRV----SNLS 120
            FS+TCHKTD NRP KA LMHT+I ++N+S NGELH L  +V+NCY      +    +++S
Sbjct: 1059 FSITCHKTDRNRPPKALLMHTNISVMNISTNGELHVLRPIVRNCYSHGDNYLDMNDADIS 1118

Query: 121  VPDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCI--------------SGSGCCQ 180
            VPDM+PLSRSKNKFIT+GCNHVGLI+GD  G + ES CI              SGSGCCQ
Sbjct: 1119 VPDMFPLSRSKNKFITIGCNHVGLILGDDQGTNDESWCISVCTRKSSVVDGLCSGSGCCQ 1178

Query: 181  LEIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVVLG 240
            L+IP G   L LAVGEL N  +V  F+PC YAFIIEA RFKFLS +I  F+++EVEVVL 
Sbjct: 1179 LDIPKGFTKLSLAVGELFNYPEVRKFSPCGYAFIIEAARFKFLSRYIDKFEEEEVEVVLS 1238

Query: 241  WGIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHN 300
            WGIRN+L+ +CG N  R NSIFNGT YRC+CLDGY G                       
Sbjct: 1239 WGIRNQLKFECGSNTTR-NSIFNGTQYRCKCLDGYEG----------------------- 1298

Query: 301  RPKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDSTS 360
             P       DVDEC Y  LNDC HK KC NTEGNYTC CPKNFHGDGRK G+GC  +STS
Sbjct: 1299 NPYLPHGCQDVDECTYPWLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTS 1358

Query: 361  SIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDI 420
            SIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNG FVLQRQLSQCNSPKDI
Sbjct: 1359 SIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDI 1418

Query: 421  VRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
            VRIFSQ+ELEKATNNYA+DTIAGKGGYGTVYKGVL+D
Sbjct: 1419 VRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDD 1427

BLAST of CmaCh04G024030 vs. ExPASy TrEMBL
Match: A0A6J1JL32 (putative wall-associated receptor kinase-like 16 OS=Cucurbita maxima OX=3661 GN=LOC111486859 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 5.8e-157
Identity = 297/456 (65.13%), Postives = 330/456 (72.37%), Query Frame = 0

Query: 1   MKPIMAFMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNF 60
           MK IMAFMVMM IAILST LA+ ASQALP C++RCGDV IPYPFGIK GCY NQ    NF
Sbjct: 1   MKHIMAFMVMMEIAILSTALAMVASQALPRCDDRCGDVQIPYPFGIKEGCYLNQ----NF 60

Query: 61  SVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDA----FTRVSNLSV 120
           S+TC+KTD N P KA+LM T+I + N+S NGELH L  VV+ CY  A        SN SV
Sbjct: 61  SITCNKTDRNGPPKAYLMQTNISVTNISTNGELHVLQPVVRTCYDKANGSFVPNESNFSV 120

Query: 121 PDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCI--------------SGSGCCQL 180
           P M+P+S +KNKF+T+GCN  GLI G      + SGCI              SGSGCCQL
Sbjct: 121 PAMFPISGTKNKFVTIGCNTAGLIAGYLQESLYGSGCISLCLNKSGVVNGSCSGSGCCQL 180

Query: 181 EIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVVLGW 240
           EIP+GL NL LAV  LLN  DV  FN C YAF+IEA+RF+FLS +I+NF ++EVEVVL W
Sbjct: 181 EIPNGLTNLSLAVTGLLNFADVRTFNHCGYAFVIEADRFQFLSRYIENF-EEEVEVVLDW 240

Query: 241 GIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNR 300
           GIRNE  ++CG NARR NSIFNGT YRCQCLDGY G                        
Sbjct: 241 GIRNETRIECGSNARRTNSIFNGTQYRCQCLDGYEG-----------------------N 300

Query: 301 PKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDSTSS 360
           P       DVDECKY  LNDC HK KC NTEGNYTC CPKNFHGDGRK G+GC  +STSS
Sbjct: 301 PYLRHGCQDVDECKYPWLNDCKHKDKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTSS 360

Query: 361 IPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIV 420
           IPIIIGIG+GL VLLIATTTIYL YKKLKFIKQKQ FF KNG FVLQRQLSQ NSP D+V
Sbjct: 361 IPIIIGIGVGLPVLLIATTTIYLGYKKLKFIKQKQLFFQKNGGFVLQRQLSQWNSPNDMV 420

Query: 421 RIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
           RIFSQ+ELEKATNNYAQDTIAGKGG+GTVYKGVL+D
Sbjct: 421 RIFSQEELEKATNNYAQDTIAGKGGFGTVYKGVLDD 428

BLAST of CmaCh04G024030 vs. ExPASy TrEMBL
Match: A0A0A0KDF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G351870 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 1.0e-121
Identity = 228/452 (50.44%), Postives = 295/452 (65.27%), Query Frame = 0

Query: 8   MVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNFSVTCHKT 67
           ++ +++ ILST + VA+SQALP C+E CGDV IPYPFG+K GCY NQ     FS+TC+KT
Sbjct: 8   VIRVVVIILSTAIVVASSQALPDCDEWCGDVQIPYPFGVKQGCYLNQ----TFSITCNKT 67

Query: 68  DGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDAFT------RVSNLSVPDMYP 127
               P KAFLM+T+I + N+S NGELH L  +V++CY            V++L VP M+P
Sbjct: 68  VS--PPKAFLMNTNISVTNISLNGELHILQPIVRDCYEQGIVIGSSVPTVTDLLVPAMFP 127

Query: 128 LSRSKNKFITLGCNHVGLIMGDYLGGDFESGCIS--------------GSGCCQLEIPDG 187
           ++  KNKFI +GC+  GLI G+  G  + SGCIS              G+GCC+LEIP+ 
Sbjct: 128 IADGKNKFIAIGCDTFGLIGGELNGSGYVSGCISMCLNESMIGNDTCLGNGCCELEIPNE 187

Query: 188 LRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVVLGWGIRNE 247
           L NL+L VG   N + V +FNPC YAF++  E F+F S +I++F+D EVEVV+GW I N 
Sbjct: 188 LMNLKLLVGSFFNHSSVKDFNPCGYAFVVGNEGFEFDSKYIRSFQDVEVEVVVGWAIGNG 247

Query: 248 LELDCGLNARRNNSIFNGTH-YRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNRPKRT 307
               CGLN++RN S  N  H +RCQCL+G+ G                        P   
Sbjct: 248 SNYVCGLNSKRNYSFSNDGHEFRCQCLEGFQG-----------------------NPYLP 307

Query: 308 ISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDSTSSIPII 367
               D+DECK   LN C + +KC+NT GNYTC+CPKNF GDGR  G GC  DS + IPII
Sbjct: 308 QGCQDIDECKDETLNQCKYNSKCVNTIGNYTCKCPKNFKGDGRNEGVGCTRDSKTFIPII 367

Query: 368 IGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIVRIFS 427
           IG+G+G  V +I +T I+L YKK KFIK+K++FF +NG FVLQRQLSQ  SP ++VR+F+
Sbjct: 368 IGVGVGFTVFVIGSTWIFLGYKKWKFIKRKEKFFEENGGFVLQRQLSQWQSPNEMVRVFT 427

Query: 428 QQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
           Q+ELEKAT +Y   TI GKGGYGTVYKGVLED
Sbjct: 428 QEELEKATKHYDNSTIVGKGGYGTVYKGVLED 430

BLAST of CmaCh04G024030 vs. NCBI nr
Match: XP_022959549.1 (uncharacterized protein LOC111460588 [Cucurbita moschata])

HSP 1 Score: 643.3 bits (1658), Expect = 1.5e-180
Identity = 329/456 (72.15%), Postives = 356/456 (78.07%), Query Frame = 0

Query: 1    MKPIMAFMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNF 60
            MKPIMAF+VMM IAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK GCY NQ    NF
Sbjct: 1098 MKPIMAFLVMMNIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIKEGCYLNQ----NF 1157

Query: 61   SVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDAFTRV----SNLSV 120
            S+ C KTD N P KAFLMHT+I ++N+  NGELH L  +V+NCY D  + +    S LSV
Sbjct: 1158 SIACDKTDRNGPPKAFLMHTNISVMNIFTNGELHVLQPIVRNCYSDGGSYLDMNDSYLSV 1217

Query: 121  PDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCI--------------SGSGCCQL 180
            PDMYPLSRSKNKFIT+GCNHVGLI+GDYLGGDFESGCI              SGSGCCQL
Sbjct: 1218 PDMYPLSRSKNKFITIGCNHVGLILGDYLGGDFESGCISMCTKKSSVADGSCSGSGCCQL 1277

Query: 181  EIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVVLGW 240
            EIP GL NLRLAVGELLN T++   +PC YAFIIEAERF+FLSS+I  F+D++V VVL W
Sbjct: 1278 EIPKGLTNLRLAVGELLNYTEIPKLSPCGYAFIIEAERFRFLSSYIDKFEDEKVVVVLSW 1337

Query: 241  GIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNR 300
            GIRNEL+ +CG N  R  SIFNGT Y CQCLDGY G                        
Sbjct: 1338 GIRNELKFECGSNTAR-ISIFNGTQYGCQCLDGYEG-----------------------N 1397

Query: 301  PKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDSTSS 360
            P       DV+ECKY  LNDCMHKAKCINTEGNYTCRCPKNFHGDGRK GEGC  +STSS
Sbjct: 1398 PYLRHGCQDVNECKYGWLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKGGEGCTKNSTSS 1457

Query: 361  IPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIV 420
            IPIIIGIGLGL VLLIA TTIYLCYKKLKFIKQKQRFFHKNG FVLQRQLSQCNSPKDIV
Sbjct: 1458 IPIIIGIGLGLVVLLIAITTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIV 1517

Query: 421  RIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
            RIFSQ+ELEKATNNYA+DTIAGKGGYGTVYKGVL+D
Sbjct: 1518 RIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDD 1525

BLAST of CmaCh04G024030 vs. NCBI nr
Match: XP_023512405.1 (wall-associated receptor kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 631.7 bits (1628), Expect = 4.7e-177
Identity = 322/456 (70.61%), Postives = 354/456 (77.63%), Query Frame = 0

Query: 1   MKPIMAFMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNF 60
           MKPIMAF+V+M IAI+STP AVAASQALPGCEERCGDVHIPYPFGIK GCY NQ    NF
Sbjct: 5   MKPIMAFLVIMNIAIISTPFAVAASQALPGCEERCGDVHIPYPFGIKEGCYLNQ----NF 64

Query: 61  SVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDAFTRV----SNLSV 120
           S+ C KTD N P KAFLMHT+I ++N+  NGELH L  +V+NCYRD  + +    S LSV
Sbjct: 65  SIACDKTDRNGPPKAFLMHTNISVMNIFTNGELHVLQPIVRNCYRDGGSYLDMNDSYLSV 124

Query: 121 PDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCI--------------SGSGCCQL 180
           PDMYPLSRSKNKFIT+GCNHVGLI+GDYLGGDFESGCI              SGSGCCQL
Sbjct: 125 PDMYPLSRSKNKFITIGCNHVGLILGDYLGGDFESGCISMCTKKSSVADGSCSGSGCCQL 184

Query: 181 EIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVVLGW 240
           EIP GL N+RLAVGELLN T++   +PC YAFIIEAERF+FLSS+I  F++++V VVL W
Sbjct: 185 EIPKGLTNVRLAVGELLNYTEIPKLSPCGYAFIIEAERFRFLSSYIDKFEEEKVVVVLSW 244

Query: 241 GIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNR 300
           GIRNEL+ +CG N  R  SIFNGT Y CQCLDGY G                        
Sbjct: 245 GIRNELKFECGSNTAR-ISIFNGTQYGCQCLDGYEG-----------------------N 304

Query: 301 PKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDSTSS 360
           P       DV+ECKY  LNDCM KAKCINTEGNYTCRCPKNFHGDGR+ GEGC  +STSS
Sbjct: 305 PYLRHGCQDVNECKYGWLNDCMPKAKCINTEGNYTCRCPKNFHGDGREGGEGCTKNSTSS 364

Query: 361 IPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIV 420
           IPIIIGIGLGL VLLIA TTIYLCYKKLKFIKQKQRFFHKNG FVLQRQLSQCNSPKDIV
Sbjct: 365 IPIIIGIGLGLVVLLIAITTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIV 424

Query: 421 RIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
           RIFSQ+ELEKATNNYA+DTIAG GGYGTVYKGVL+D
Sbjct: 425 RIFSQEELEKATNNYAKDTIAGNGGYGTVYKGVLDD 432

BLAST of CmaCh04G024030 vs. NCBI nr
Match: XP_023542638.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111802455 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615.5 bits (1586), Expect = 3.4e-172
Identity = 316/456 (69.30%), Postives = 350/456 (76.75%), Query Frame = 0

Query: 1    MKPIMAFMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGI-KGCYFNQNSSVNF 60
            +KPIM F+VMM IAILSTPL V AS+ALPGC+E CG VHIPYPFGI KGCY NQ    NF
Sbjct: 737  IKPIMTFLVMMNIAILSTPLVVEASEALPGCDEWCGKVHIPYPFGIKKGCYLNQ----NF 796

Query: 61   SVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYR--DAFTRV--SNLSV 120
            S+TCHKT G    +AFLMHT+IR++N+S NGELH L  +V+NCY   D +  +  ++LSV
Sbjct: 797  SITCHKT-GPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETDLSV 856

Query: 121  PDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCI--------------SGSGCCQL 180
            P MYPLSRSKNKFIT+GCNHVGLI+GD  G + ESGCI              SGSGCCQL
Sbjct: 857  PTMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRSSVVDGLCSGSGCCQL 916

Query: 181  EIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVVLGW 240
            +IP GL NL LAVGELLN T++  F+PC YAFIIEA+RF+FLSS+I  F++KEVEVVL W
Sbjct: 917  DIPKGLTNLSLAVGELLNYTEIRKFSPCGYAFIIEAKRFQFLSSYIDKFEEKEVEVVLSW 976

Query: 241  GIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNR 300
            GIR ELELDCGLNARRNNSIF+ THYRCQCLDGY G                        
Sbjct: 977  GIRKELELDCGLNARRNNSIFSETHYRCQCLDGYQG-----------------------N 1036

Query: 301  PKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDSTSS 360
            P       DVDEC Y  LNDC HK KC NTEGNYTC CPKNFHGDGRK G+GC  + TSS
Sbjct: 1037 PYLPQGCQDVDECTYPWLNDCEHKDKCSNTEGNYTCHCPKNFHGDGRKGGKGCTQNPTSS 1096

Query: 361  IPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIV 420
            IPIIIGIGLGL VLLIA TTIYLCYKKLKFIKQKQRFFHKNG FVLQRQLSQCNSPKDIV
Sbjct: 1097 IPIIIGIGLGLVVLLIAITTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDIV 1156

Query: 421  RIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
            RIFSQ+ELEKATNNYA+DTIAGKGGYGTVYKGVL+D
Sbjct: 1157 RIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDD 1164

BLAST of CmaCh04G024030 vs. NCBI nr
Match: XP_022989772.1 (wall-associated receptor kinase 3-like [Cucurbita maxima])

HSP 1 Score: 613.6 bits (1581), Expect = 1.3e-171
Identity = 320/459 (69.72%), Postives = 352/459 (76.69%), Query Frame = 0

Query: 1   MKPIMAFMVMMI-IAILSTPLAVAASQALPGCEERCGDVHIPYPFGI-KGCYFNQNSSVN 60
           +KPIMAFMVMM+ I ILST LAV AS+ALPGC+E CG VHIPYPFGI KGCY NQ    N
Sbjct: 5   IKPIMAFMVMMMKIGILSTALAVEASEALPGCDEWCGKVHIPYPFGIKKGCYLNQ----N 64

Query: 61  FSVTCHKTDGNRPLK--AFLMHTHIRIINLSANGELHALGRVVQNCYR--DAFTRV--SN 120
           FS+TCHKTD N P K  AFLMHT+IR++N+S NGELH L  +V+NCY   D +  +  ++
Sbjct: 65  FSITCHKTDLNGPPKAEAFLMHTNIRVMNISTNGELHVLRPIVRNCYSYGDNYLDMNETD 124

Query: 121 LSVPDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCI--------------SGSGC 180
           LSVP MYPLSRSKNKFIT+GCNHVGLI+GD  G + ESGCI              SGSGC
Sbjct: 125 LSVPTMYPLSRSKNKFITIGCNHVGLILGDDQGTNVESGCISVCTRRISVVDGLCSGSGC 184

Query: 181 CQLEIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVV 240
           CQLEIP GL  L LAVGELLN T++  F+PC YAFIIEA RF+FLSS+I  F++KEVEVV
Sbjct: 185 CQLEIPKGLTKLSLAVGELLNYTEIRKFSPCGYAFIIEARRFQFLSSYIDKFEEKEVEVV 244

Query: 241 LGWGIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAI 300
           L WGIR ELELDCGLNARRN+SIFNGTHYRCQCLDGY G                     
Sbjct: 245 LSWGIRKELELDCGLNARRNSSIFNGTHYRCQCLDGYEG--------------------- 304

Query: 301 HNRPKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDS 360
              P       DVDECKY  LNDC HK KC NTEGNYTC CPKNFHGDGRK G+GC  +S
Sbjct: 305 --NPYLRHGCQDVDECKYPWLNDCKHKDKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNS 364

Query: 361 TSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPK 420
           TSSIPIIIGIG+GL VLLIATTTIYL YKKLKFIKQKQ FF KNG FVLQRQLSQCNSPK
Sbjct: 365 TSSIPIIIGIGVGLPVLLIATTTIYLGYKKLKFIKQKQLFFQKNGGFVLQRQLSQCNSPK 424

Query: 421 DIVRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
           DIVRIFSQ+ELEKATNNYAQDTIAGKGGYGTV+KGVL+D
Sbjct: 425 DIVRIFSQEELEKATNNYAQDTIAGKGGYGTVFKGVLDD 436

BLAST of CmaCh04G024030 vs. NCBI nr
Match: XP_022959563.1 (uncharacterized protein LOC111460594 [Cucurbita moschata])

HSP 1 Score: 602.1 bits (1551), Expect = 3.9e-168
Identity = 311/457 (68.05%), Postives = 345/457 (75.49%), Query Frame = 0

Query: 1    MKPIMAFMVMMI-IAILSTPLAVAASQALPGCEERCGDVHIPYPFGI-KGCYFNQNSSVN 60
            +KPIMAFMVMM+ IAILST LAVAASQALPGC+E CG VHIPYPFGI KGCY NQ    N
Sbjct: 999  IKPIMAFMVMMMKIAILSTALAVAASQALPGCDEWCGKVHIPYPFGIKKGCYLNQ----N 1058

Query: 61   FSVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDAFTRV----SNLS 120
            FS+TCHKTD NRP KA LMHT+I ++N+S NGELH L  +V+NCY      +    +++S
Sbjct: 1059 FSITCHKTDRNRPPKALLMHTNISVMNISTNGELHVLRPIVRNCYSHGDNYLDMNDADIS 1118

Query: 121  VPDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCI--------------SGSGCCQ 180
            VPDM+PLSRSKNKFIT+GCNHVGLI+GD  G + ES CI              SGSGCCQ
Sbjct: 1119 VPDMFPLSRSKNKFITIGCNHVGLILGDDQGTNDESWCISVCTRKSSVVDGLCSGSGCCQ 1178

Query: 181  LEIPDGLRNLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKDKEVEVVLG 240
            L+IP G   L LAVGEL N  +V  F+PC YAFIIEA RFKFLS +I  F+++EVEVVL 
Sbjct: 1179 LDIPKGFTKLSLAVGELFNYPEVRKFSPCGYAFIIEAARFKFLSRYIDKFEEEEVEVVLS 1238

Query: 241  WGIRNELELDCGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHN 300
            WGIRN+L+ +CG N  R NSIFNGT YRC+CLDGY G                       
Sbjct: 1239 WGIRNQLKFECGSNTTR-NSIFNGTQYRCKCLDGYEG----------------------- 1298

Query: 301  RPKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDSTS 360
             P       DVDEC Y  LNDC HK KC NTEGNYTC CPKNFHGDGRK G+GC  +STS
Sbjct: 1299 NPYLPHGCQDVDECTYPWLNDCEHKNKCSNTEGNYTCHCPKNFHGDGRKGGKGCTKNSTS 1358

Query: 361  SIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDI 420
            SIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNG FVLQRQLSQCNSPKDI
Sbjct: 1359 SIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGGFVLQRQLSQCNSPKDI 1418

Query: 421  VRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
            VRIFSQ+ELEKATNNYA+DTIAGKGGYGTVYKGVL+D
Sbjct: 1419 VRIFSQEELEKATNNYAKDTIAGKGGYGTVYKGVLDD 1427

BLAST of CmaCh04G024030 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 173.7 bits (439), Expect = 3.2e-43
Identity = 135/465 (29.03%), Postives = 210/465 (45.16%), Query Frame = 0

Query: 9   VMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNFSVTCHKTD 68
           +++I  +  T L     Q    C+ +CG+V I YPFGI  GCY+  +   NF++TC   +
Sbjct: 9   LVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDD--NFNLTCVVEE 68

Query: 69  GNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCY---RDAFTRVSNLSVPDMYPLSRS 128
                   L+   I++ N+S +G +  L      CY    +         +   + LS S
Sbjct: 69  ------KLLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETNGTALGYQLGSSFSLS-S 128

Query: 129 KNKFITLGCNHVGLIMGDYLGGDFESGCIS-------------GSGCCQLE--------- 188
            NKF  +GCN + L +  +   ++ +GC+S             G GCC  E         
Sbjct: 129 NNKFTLVGCNALSL-LSTFGKQNYSTGCLSLCNSQPEANGRCNGVGCCTTEDFSVPFDSD 188

Query: 189 -IPDGLRNLRLAVGELLN--STDVWNFNPCWYAFIIEAERFKFLSS-FIKNFKD-KEVEV 248
               G   LR  V   L+  +T V+ FNPC YAF++E  +F F SS  +KN ++     V
Sbjct: 189 TFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTRFPV 248

Query: 249 VLGWGIRNELELDCGLN--ARRNNSIFNGT---HYRCQCLDGYGGIHISLKDVKKTRFKT 308
            L W I N+     G      +N+S +N T    Y C+C +GY G               
Sbjct: 249 ALDWSIGNQTCEQAGSTRICGKNSSCYNSTTRNGYICKCNEGYDG--------------- 308

Query: 309 VRLTAIHNRPKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGE 368
                    P R+    D+DEC  +  ++C     C N +G + C+CP    G       
Sbjct: 309 --------NPYRSEGCKDIDEC-ISDTHNCSDPKTCRNRDGGFDCKCPS---GYDLNSSM 368

Query: 369 GCNPDSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLS 428
            C         I + I +G+ VLL+A   I    K+ K+ K +++FF +NG  +L ++LS
Sbjct: 369 SCTRPEYKRTRIFLVIIIGVLVLLLAAICIQHATKQRKYTKLRRQFFEQNGGGMLIQRLS 428

Query: 429 QCNSPKDIVRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
                    +IF+++ +++ATN Y +  I G+GG GTVYKG+L D
Sbjct: 429 GAGLSNIDFKIFTEEGMKEATNGYDESRILGQGGQGTVYKGILPD 436

BLAST of CmaCh04G024030 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 172.2 bits (435), Expect = 9.4e-43
Identity = 124/435 (28.51%), Postives = 205/435 (47.13%), Query Frame = 0

Query: 31  CEERCGDVHIPYPFGIK-GCYFNQNSSVNFSVTCHKTDGNRPLKAFLMHTHIRIINLSAN 90
           C+ RCG+V + YPFG   GCY+  + S  F++TC++ +        L   ++ +IN+S +
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDES--FNLTCNEQEK-------LFFGNMPVINMSLS 88

Query: 91  GELHALGRVVQN--CYRDAFTR---VSNLSVPDMYPLSRSKNKFITLGCNHVGLIMGDYL 150
           G+L    R+V++  CY     +   ++  +    + LS   N+F  +GCN    +    +
Sbjct: 89  GQLRV--RLVRSRVCYDSQGKQTDYIAQRTTLGNFTLS-ELNRFTVVGCNSYAFLRTSGV 148

Query: 151 GGDFESGCI-------------SGSGCCQLEIPDGLRNLRLAVGELLNSTDVWNFNPCWY 210
              + +GCI             SG GCCQ+ +P G   +R+      N   V  FNPC Y
Sbjct: 149 -EKYSTGCISICDSATTKNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTY 208

Query: 211 AFIIEAERFKFLS-SFIKNFKD-KEVEVVLGWGIRN------ELELDCGLNARRNNSIFN 270
           AF++E   F F +   + N ++     VVL W I +      E    CG N+   +S   
Sbjct: 209 AFLVEDGMFDFHALEDLNNLRNVTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDST-G 268

Query: 271 GTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNRPKRTISASDVDECKYARLNDCM 330
           GT Y C+CL+G+ G                        P       D++EC  +R N C 
Sbjct: 269 GTGYNCKCLEGFEG-----------------------NPYLPNGCQDINECISSRHN-CS 328

Query: 331 HKAKCINTEGNYTCRCPKNFHGDG-RKRGEGCNPDSTSSIPIIIGIGLGLAVLLIATTTI 390
             + C NT+G++ C CP  +  D          P+      I +G  +G +V+++  + +
Sbjct: 329 EHSTCENTKGSFNCNCPSGYRKDSLNSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCL 388

Query: 391 YLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIVRIFSQQELEKATNNYAQDTIA 438
               K  K  + +Q+FF +NG  +L +++S        V+IF+++ +++ATN Y +  I 
Sbjct: 389 QQKIKHRKNTELRQKFFEQNGGGMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRIL 425

BLAST of CmaCh04G024030 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 166.8 bits (421), Expect = 3.9e-41
Identity = 133/476 (27.94%), Postives = 214/476 (44.96%), Query Frame = 0

Query: 1   MKPIMAFMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNF 60
           MK    F++ +   +  T L  A  Q    C+ RCGDV I YPFGI  GCY+  + S  F
Sbjct: 1   MKVHSLFLMAIFFYLAYTQLVKA--QPRDDCQTRCGDVPIDYPFGISTGCYYPGDDS--F 60

Query: 61  SVTCHKTDGNRPLKAFLMHTHIRIINLSANGELHAL--------GRVVQNCYRDAFTRVS 120
           ++TC +   N       + ++I ++N + +G+L  L         +   N +   + R+ 
Sbjct: 61  NITCEEDKPN-------VLSNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLD 120

Query: 121 NLSVPDMYPLSRSKNKFITLGCNHVGLIMGDYLGGDFESGCIS-------------GSGC 180
           NLS           NKF  +GCN    ++  +   ++ +GC+S             G GC
Sbjct: 121 NLSF-------SPNNKFTLVGCN-AWALLSTFGIQNYSTGCMSLCDTPPPPNSKCNGVGC 180

Query: 181 CQLEIPDGLRNLRLAV--GELLNSTDVWNFNPCWYAFIIEAERFKFLSSFIKNFKD---- 240
           C+ E+   L + R+        N T V +FNPC YAF +E   F F  S +++ KD    
Sbjct: 181 CRTEVSIPLDSHRIETQPSRFENMTSVEHFNPCSYAFFVEDGMFNF--SSLEDLKDLRNV 240

Query: 241 KEVEVVLGWGIRNE-LELDCGLNARRNNSI----FNGTHYRCQCLDGYGGIHISLKDVKK 300
               V+L W I N+  E   G N    NS       G  Y C+CL G+ G          
Sbjct: 241 TRFPVLLDWSIGNQTCEQVVGRNICGGNSTCFDSTRGKGYNCKCLQGFDG---------- 300

Query: 301 TRFKTVRLTAIHNRPKRTISASDVDECKYARLNDCMHKAKCINTEGNYTCRCPKNFHGDG 360
                         P  +    D++EC   R+++C   + C NT G++ C+CP     D 
Sbjct: 301 -------------NPYLSDGCQDINECT-TRIHNCSDTSTCENTLGSFHCQCPSG--SDL 360

Query: 361 RKRGEGC------NPDSTSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHK 420
                 C       P       +++G  +G  ++L+  + I    +  K  + +Q+FF +
Sbjct: 361 NTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHRKNTELRQQFFEQ 420

Query: 421 NGAFVLQRQLSQCNSPKDIVRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
           NG  +L ++LS        V+IF+++ +++AT+ Y +  I G+GG GTVYKG+L+D
Sbjct: 421 NGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVYKGILQD 429

BLAST of CmaCh04G024030 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 166.8 bits (421), Expect = 3.9e-41
Identity = 126/459 (27.45%), Postives = 209/459 (45.53%), Query Frame = 0

Query: 7   FMVMMIIAILSTPLAVAASQALPGCEERCGDVHIPYPFGI-KGCYFNQNSSVNFSVTCHK 66
           F+V +  ++  T L     Q    C+ +CG++ I YPFGI  GCY+  N S  FS+TC +
Sbjct: 8   FLVAIFFSLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNES--FSITCKE 67

Query: 67  TDGNRPLKAFLMHTHIRIINLSANGELHALGRVVQNCYRDAFTRVSNLSVPDMYPLSRS- 126
              +RP     + + I + N + +G+L  L      CY +   +    S   +  LS S 
Sbjct: 68  ---DRP----HVLSDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSA 127

Query: 127 KNKFITLGCNHVGLIMGDYLG-GDFESGCIS-------------GSGCCQLEI--PDGLR 186
            NK   +GCN + L+  D  G  ++ + C+S             G GCC++++  P    
Sbjct: 128 NNKLTAVGCNALSLL--DTFGMQNYSTACLSLCDSPPEADGECNGRGCCRVDVSAPLDSY 187

Query: 187 NLRLAVGELLNSTDVWNFNPCWYAFIIEAERFKFLSS-FIKNFKD-KEVEVVLGWGIRNE 246
                 G + + T   +F+PC YAF++E ++F F S+  + N ++     V+L W + N+
Sbjct: 188 TFETTSGRIKHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQ 247

Query: 247 LELDCGLNA--RRNNSIFNGT---HYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNR 306
                G  +    N++  + T    Y C+C +G+ G                        
Sbjct: 248 TCEQVGSTSICGGNSTCLDSTPRNGYICRCNEGFDG-----------------------N 307

Query: 307 PKRTISASDVDECKYA---RLNDCMHKAKCINTEGNYTCRCPKNFHGDGRKRGEGCNPDS 366
           P  +    DV+EC  +     ++C     C N  G + C+C   +  D       C    
Sbjct: 308 PYLSAGCQDVNECTTSSTIHRHNCSDPKTCRNKVGGFYCKCQSGYRLDTTTM--SCKRKE 367

Query: 367 TSSIPIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPK 426
            +   I++   +G  V+L+    I    K LK  K +++FF +NG  +L ++LS      
Sbjct: 368 FAWTTILLVTTIGFLVILLGVACIQQRMKHLKDTKLREQFFEQNGGGMLTQRLSGAGPSN 427

Query: 427 DIVRIFSQQELEKATNNYAQDTIAGKGGYGTVYKGVLED 438
             V+IF++  ++KATN YA+  I G+GG GTVYKG+L D
Sbjct: 428 VDVKIFTEDGMKKATNGYAESRILGQGGQGTVYKGILPD 430

BLAST of CmaCh04G024030 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 155.6 bits (392), Expect = 9.1e-38
Identity = 125/455 (27.47%), Postives = 203/455 (44.62%), Query Frame = 0

Query: 20  LAVAASQALPGCEERCGDVHIPYPFGIK-GCYFNQNSSVNFSVTCHKTDGNRPLKAFLMH 79
           + +   Q LP C E+CG+V + YPFG   GC+  ++ S N S           +   L +
Sbjct: 17  MQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSC----------VNENLFY 76

Query: 80  THIRIINLSANGELHALGRVVQNCY--RDAFTRVSNLSVPDMYPLSRSKNKFIT-LGCNH 139
             + ++ +S + +L  L      CY  +  F +       ++  L+ S N  IT LGCN 
Sbjct: 77  KGLEVVEISHSSQLRVLYPASYICYNSKGKFAK-GTYYWSNLGNLTLSGNNTITALGCNS 136

Query: 140 VGLIMGDYLGGDFESGCIS--------------GSGCCQLEIPDGLRNLRLAVGELLNST 199
              +  +    +   GCIS              G GCCQ  +P G   L +      N T
Sbjct: 137 YAFVSSNGTRRN-SVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLIVRSYRFDNDT 196

Query: 200 DVWNFN--PCWYAFIIEAERFKFLS----SFIKNFKDKEVEVVLGWGIRNEL-----ELD 259
            V   +   C YAF++E  +FK+ +    S+++N ++    VVL W IR E      E  
Sbjct: 197 SVQPISEGQCIYAFLVENGKFKYNASDKYSYLQN-RNVGFPVVLDWSIRGETCGQVGEKK 256

Query: 260 CGLNARRNNSIFNGTHYRCQCLDGYGGIHISLKDVKKTRFKTVRLTAIHNRPKRTISASD 319
           CG+N   +NS  +G  Y C+C  G+ G                        P       D
Sbjct: 257 CGVNGICSNSA-SGIGYTCKCKGGFQG-----------------------NPYLQNGCQD 316

Query: 320 VDECKYA---RLNDCMHKAKCINTEGNYTCRCPKNFH-----GDGRKRGEGCNPDSTSSI 379
           ++EC  A     ++C   + C N  G++ C C   +         + +G   NP+     
Sbjct: 317 INECTTANPIHKHNCSGDSTCENKLGHFRCNCRSRYELNTTTNTCKPKG---NPEYVEWT 376

Query: 380 PIIIGIGLGLAVLLIATTTIYLCYKKLKFIKQKQRFFHKNGAFVLQRQLSQCNSPKDIVR 438
            I++G  +G  V+L+A + I    K  K  + +Q+FF +NG  +L ++LS        V+
Sbjct: 377 TIVLGTTIGFLVILLAISCIEHKMKNTKDTELRQQFFEQNGGGMLMQRLSGAGPSNVDVK 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMN84.5e-4229.03Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 S... [more]
Q9LMP11.3e-4128.51Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 S... [more]
Q391915.6e-4027.45Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 S... [more]
Q9LMN75.6e-4027.94Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 S... [more]
Q9LMN61.3e-3627.47Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1H8D67.5e-18172.15uncharacterized protein LOC111460588 OS=Cucurbita moschata OX=3662 GN=LOC1114605... [more]
A0A6J1JGR16.3e-17269.72wall-associated receptor kinase 3-like OS=Cucurbita maxima OX=3661 GN=LOC1114868... [more]
A0A6J1H6B01.9e-16868.05uncharacterized protein LOC111460594 OS=Cucurbita moschata OX=3662 GN=LOC1114605... [more]
A0A6J1JL325.8e-15765.13putative wall-associated receptor kinase-like 16 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A0A0KDF01.0e-12150.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G351870 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022959549.11.5e-18072.15uncharacterized protein LOC111460588 [Cucurbita moschata][more]
XP_023512405.14.7e-17770.61wall-associated receptor kinase 1-like [Cucurbita pepo subsp. pepo][more]
XP_023542638.13.4e-17269.30LOW QUALITY PROTEIN: uncharacterized protein LOC111802455 [Cucurbita pepo subsp.... [more]
XP_022989772.11.3e-17169.72wall-associated receptor kinase 3-like [Cucurbita maxima][more]
XP_022959563.13.9e-16868.05uncharacterized protein LOC111460594 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G21240.13.2e-4329.03wall associated kinase 3 [more]
AT1G21270.19.4e-4328.51wall-associated kinase 2 [more]
AT1G21230.13.9e-4127.94wall associated kinase 5 [more]
AT1G21250.13.9e-4127.45cell wall-associated kinase [more]
AT1G21210.19.1e-3827.47wall associated kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001881EGF-like calcium-binding domainSMARTSM00179egfca_6coord: 290..335
e-value: 5.3E-7
score: 39.3
IPR001881EGF-like calcium-binding domainPFAMPF07645EGF_CAcoord: 290..327
e-value: 2.1E-8
score: 34.2
NoneNo IPR availableGENE3D2.10.25.10Laminincoord: 276..330
e-value: 1.9E-11
score: 45.6
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 380..437
e-value: 2.7E-8
score: 35.5
NoneNo IPR availablePANTHERPTHR27005WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21coord: 16..437
NoneNo IPR availablePANTHERPTHR27005:SF341WALL-ASSOCIATED RECEPTOR KINASE 2-LIKE ISOFORM X2coord: 16..437
NoneNo IPR availableCDDcd00054EGF_CAcoord: 290..326
e-value: 7.11606E-8
score: 46.4758
NoneNo IPR availableSUPERFAMILY57196EGF/Laminincoord: 287..326
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 31..84
e-value: 7.4E-7
score: 29.5
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 290..317
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 308..319
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 290..326
score: 13.864076

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G024030.1CmaCh04G024030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004672 protein kinase activity