CmaCh04G023530 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G023530
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionaminoacylase-1
LocationCma_Chr04: 16323465 .. 16327983 (-)
RNA-Seq ExpressionCmaCh04G023530
SyntenyCmaCh04G023530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCGAGTTGAAGAGGCAAAAAGCCAAGTTTTGACAATCCATTCATCGGCGGTTGTCTTTACCATTCGGCAGCTCTCCGGCTTACTCAAGTTCAAAAGTTCCGACTAGCTGCAACTGCTTGATCGCCGCACCAACGGAGGCGCCCATGGCGAACTTCCGTCTCCACTGTCGGCTCTTTTCTCTTGCAGTTTTTCTTTTTCTCTTCTTATTTCCTCTCTCTTTCAGTCATTCGCAGTACGATGATGATGAATCCGATCAAAACTCTCCAATCTCTCGCTTTCAACACTACCTCAGAATCAAAACCGCTCATCCGGATCCGGATTACGCCTCCGCAGTTTCCTTTCTCGAATCTCAAGCACTGGAAATTGGTCTCCATACGAAGATTCTTGAGTTTGTTCCAGGTAAACCTCTTCTCCTTGTGACCTGGAATGGATTGAATCCTTCTTTGCCCTCGATTTTACTCAACTCTCACATGGACTCTGTCCCCGCTGAGCCCTCTAAATGGCTCTATCCGCCGTTCTCCGCCCTCCGGACCTCCGATGGAAAGATCTTCGCCCGTGGTTCTCAAGATGATAAATGTATAGCTATCCAGTATCTCGAAGCTATTCGGAACCTCCGAAGTCGGAATTTCATCCCTGACCGTACGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTTGATGGAGCTTCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGCTGAATGTTGGATTCATGATGGACGAAGGACAGGCGTCGCCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGCTGATAATTAGGGCTGAAGGAGCCCCTGGACATGGTTCTAGAATGTACGATAACAGTGCTATGGAGAATTTGATGAAGAGCGTTGAGATTATCAGTAAGTTTAGAGAGAGCCAATTCGATATGGTGAAGGCCGGTGAAACTGCTAATTCAGAAGTCATCTCTGTCAATCCTGTTTTCGTCAACGCCGGAATTCCCTCCCCCACTGTGAGTAGTCCTTGTGAAATGAGTTGCAGGGTTCAAGCCCCTCTGCCTATGTCTCTTTTCGCCATTAATATTGTGATTTGTCTCAGGGATTTGTGATGAACATGCAACCTTCGGAGGCGGAAGCCGGTTTCGATCTCAGAATACCGCCGACCGCCGACCCAGATGCCATTAGAAGAAGAATTGTGGAGGAATGGGCTCCAGCTCGACGGAACTTGACGTCTCAGGTAAGTGGCTAGCTGCCTGATTTGATTCCCAACACCATCTGGTTCCTGTTTTATTAGCTCCCATCAATAGTATTCTGTTCTTGTTCATGGCCTATGGTTCTGCAATTTAAAGAAAAAAGCTTGGAGAAGTCCTCATATGACTCGTCATTTTATCTTGCTGAAAACAGAGTAAATATGTAAGATTATGATCTTAAACCAATTTTGATGTGGAATGAATTTGAATTCTGATGGAGGATGTTTTCGTTTGTTCTTTATGTCATATTATGAAACTCGTCTACTCCATCCACAACAAAATGCACCGTGCAATGGCATGCGAATTTTCCCTTGTCATGGGAGGAATTGATTAAATTTTCTTTTTTCTTGTGAAGATTCCAAATCGGTTGGAGAGGGGAACGAAACATTCTTTATAAGGGTGTGGAAATCTCTCCCTAGCAGATGCGTTTTAAAAATCTTGAGGGAAACTCGAAAGAGAAAGCCCAAAGAGGACAATATCTGCTAGCAGTGGGTTTGAGCCGTTACAAATGGTATTAGAGCTAGACACTAGCCGATGTGCCAACGAGGAGGTTGAGCCTCGAAGGAGGCGGACACGAGATGGTGTGCCAGCAAGGACGTTGGGCCCCGAAGGGGGGGTTAATTGGGGGGTCCCACATCGATTGGAGAAATTGAACAAGTGTCAGCGAGGACGCTGGCCCCGAAGGGAGGTGGATTGTGAGATCCCACATCAGATAAAGAGGGAAATGAAGCATTTTTTATAAGGGTGTGAAAACCTCTCCCTAACAGATAGGTTTTAAAAACCTTGAAGGGAAGCCCGAAAGAAAAAGTCCAAAGAGGACAATATCTGTTGGTAGTGAGCTTGGGCCTTTACACTTCTGCCGTACGTATGCGAGATTGATTGCCTGTTTTAGGAATGTCTAGCCTTTAGCAATCTCGTTTATTTTCCAGTCTTTAAAGGGATTAGATATTCAAGTATTTGCATCATATAGCAGTCTCTTACGAATTACACACAATCTATTTGTAAGTTATCTTAGCAATGTCTGGTAGCTGAAACATAATGGATCTGATACCTGCAGATTATTGAGAAAGGACCCATAAGAGACTATTTGGGACGTCCATTAATGACGATGACTAACGATTCGAATCCATGGTGGTCTGTTTTCAAGACAGCCATCTCAGATGCTGGAGGTAAGCTTTCTAGGCCCGAAATCTTGGCTTCGACGACTGATGCACGCTTCATGAGACAGTTAGGCATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCATGAACACAACGAGGTTATACTAGACTCCATTGTGTCTCTCTTTTTTATCCATGTTCTTGCTTGATTTGCGTTACGTTCATATTAATCGTCATCTCCTTTCCTCATTCCTTGTTACAGTTCTTGAAGGACACCGTCTTCTTAAGGGGCATAAAGATTTATGAGTCCATAATCAGTGCCTTGAGTTCCTTTGTAGCGGATGCATCCCAGTAAGTGCTCCCGTGGATGGACACTGCTATTGTAAGTACTGAAGCTCTCTTTTCCACACAACGTCACAATTCATTTGTAAACATTCTCTTTCGTTTTATTACCTTTTCATGAGATTCGAATAGGGTAAATAATAAACAAAAAAAAAATAATCTTTTGTAGTATATTGAGACTGTCTCCTCTAAGCGCATCCTTCCTAAATTAACTGGGTATCAAATAACGAGATCTATTCTATAGGTCGTAAAAATGTGTCCTCGAATTCTAAGAAGTTATATAGCTATCAACAACGAAATGTATATTGCTCGAAATAGAAGTCATGTGTCGTACACCTTACTCGATATGCTAGGACAATTTGAGGGGTTAGCTTGAGCATAAACGAAGAACCGAATTACCATGCTTAGATTGGCTCTTTATAGTTCTGACGTTGAGATTCTATTTAGAGGGTCTCAATGTTAAAAGGTCAACAATGAGACGTCTATAGTATTGCAATGCTAGTAGCCAATAAGTTCTACGTACTTGCATGAACCTCAATGCTCCGGTGGGCTTCAACGGTTGGCTCTGAGGATGAAGCAGAAGGGTTTTGCAGCAGAGCTTGTGAGAATTGTAGTGATGATGATTTTTTATCCATTTTTTGGAGCAACCTAGGAATCCACAAATGAACTGTTGGCTGTTTGAGTTGGTGTTGAGCTGTTTTGCTTGTTTAAACCCTTGTTCTCAAGTCATTCTTGATCCTGTTATCTGTTCTTTCTAAGTAAATGTTGCCCAATTTTTGGCCAATTAGAAATGAACAACAAAACAAACGAAGAAACAACCTTAAAATTTGAGACCTTGAAAAAGAAGAAGAAGAGATTGTAACAGCTATCTGATATCGTCCTTTATGGACTTTTACTTCCGGACTTTCCCTCACAGTTTTAAAATGCATACACTAGGAGAGGTTTCCACATTCTTATAAGAAATGTTTCTTTCCTCTCTCTAGTGATATAGAGAGAGAGTCTTCTTGAATGGTCCATGGGCTTCCGGGCATGTATTTTGAATGCCCACGATCTAATAAAACCATTAGCAAAGCTCAAATTTACATTTTCTGAAGAAGAAAGGAAAACCGTAGGTCAGGTCTCATAGACTATGCATGCTCCAAGCAACTCAAAGCTCGTTTTCTAAACTTAGCTTACCACACTTACGTATCAAGAGTCACCACACCCATGCATAGTTTTTCGAACACCTTCCCACAAACTCAATAGCTAAAAGTGAATAAAACCGGCTCATACAAAATGTTGAGAAAAGGGTCGACAAGAGACGATTTGGGTTATGATTCGATTCGATTCTGTAGTTGAAAATGGGCAACAAAAGCACTTGGGATGTTGCCTCTTCATCTTCCAACATCTCTCCACCTTTGAGAACCATCGCTGCAGGTTCGCCCATCCTCCTAAGCCTAATCCAAATCCAAATCCAAATCCAAATCCAAACCCTTTTCTCTATTTAACATTATTTTCTCAGAAAACAAACAAAACATTGGTCTTATACGCCATGTGCCTCTTTCACTTCATCTTCTTCCACACCACACCAACAAAAATTCATGGATCTCTGCGTTCTTGTTTCTATGCAGCGTACGGAAGCTGCTCCATTGCCAGCTGAAACCAAGAGGATAAAAGGGATAGAGATGGAGATTTGGAACTATGGGAAGAACCATTGTATCTTCCTTAAATCTTCTTCCAATCCGATTATGATTGATTTTGGTGGGTCTCGATTCAAAATTGAAAGCTTAGGGTCCCGTTAG

mRNA sequence

GGCGAGTTGAAGAGGCAAAAAGCCAAGTTTTGACAATCCATTCATCGGCGGTTGTCTTTACCATTCGGCAGCTCTCCGGCTTACTCAAGTTCAAAAGTTCCGACTAGCTGCAACTGCTTGATCGCCGCACCAACGGAGGCGCCCATGGCGAACTTCCGTCTCCACTGTCGGCTCTTTTCTCTTGCAGTTTTTCTTTTTCTCTTCTTATTTCCTCTCTCTTTCAGTCATTCGCAGTACGATGATGATGAATCCGATCAAAACTCTCCAATCTCTCGCTTTCAACACTACCTCAGAATCAAAACCGCTCATCCGGATCCGGATTACGCCTCCGCAGTTTCCTTTCTCGAATCTCAAGCACTGGAAATTGGTCTCCATACGAAGATTCTTGAGTTTGTTCCAGGTAAACCTCTTCTCCTTGTGACCTGGAATGGATTGAATCCTTCTTTGCCCTCGATTTTACTCAACTCTCACATGGACTCTGTCCCCGCTGAGCCCTCTAAATGGCTCTATCCGCCGTTCTCCGCCCTCCGGACCTCCGATGGAAAGATCTTCGCCCGTGGTTCTCAAGATGATAAATGTATAGCTATCCAGTATCTCGAAGCTATTCGGAACCTCCGAAGTCGGAATTTCATCCCTGACCGTACGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTTGATGGAGCTTCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGCTGAATGTTGGATTCATGATGGACGAAGGACAGGCGTCGCCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGCTGATAATTAGGGCTGAAGGAGCCCCTGGACATGGTTCTAGAATGTACGATAACAGTGCTATGGAGAATTTGATGAAGAGCGTTGAGATTATCAGTAAGTTTAGAGAGAGCCAATTCGATATGGTGAAGGCCGGTGAAACTGCTAATTCAGAAGTCATCTCTGTCAATCCTGTTTTCGTCAACGCCGGAATTCCCTCCCCCACTGGATTTGTGATGAACATGCAACCTTCGGAGGCGGAAGCCGGTTTCGATCTCAGAATACCGCCGACCGCCGACCCAGATGCCATTAGAAGAAGAATTGTGGAGGAATGGGCTCCAGCTCGACGGAACTTGACGTCTCAGATTATTGAGAAAGGACCCATAAGAGACTATTTGGGACGTCCATTAATGACGATGACTAACGATTCGAATCCATGGTGGTCTGTTTTCAAGACAGCCATCTCAGATGCTGGAGGTAAGCTTTCTAGGCCCGAAATCTTGGCTTCGACGACTGATGCACGCTTCATGAGACAGTTAGGCATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCATGAACACAACGAGTTCTTGAAGGACACCGTCTTCTTAAGGGGCATAAAGATTTATGAGTCCATAATCAGTGCCTTGAGTTCCTTTGTAGCGGATGCATCCCAAAAACAAACAAAACATTGGTCTTATACGCCATGTGCCTCTTTCACTTCATCTTCTTCCACACCACACCAACAAAAATTCATGGATCTCTGCGTTCTTGTTTCTATGCAGCGTACGGAAGCTGCTCCATTGCCAGCTGAAACCAAGAGGATAAAAGGGATAGAGATGGAGATTTGGAACTATGGGAAGAACCATTGTATCTTCCTTAAATCTTCTTCCAATCCGATTATGATTGATTTTGGTGGGTCTCGATTCAAAATTGAAAGCTTAGGGTCCCGTTAG

Coding sequence (CDS)

ATGGCGAACTTCCGTCTCCACTGTCGGCTCTTTTCTCTTGCAGTTTTTCTTTTTCTCTTCTTATTTCCTCTCTCTTTCAGTCATTCGCAGTACGATGATGATGAATCCGATCAAAACTCTCCAATCTCTCGCTTTCAACACTACCTCAGAATCAAAACCGCTCATCCGGATCCGGATTACGCCTCCGCAGTTTCCTTTCTCGAATCTCAAGCACTGGAAATTGGTCTCCATACGAAGATTCTTGAGTTTGTTCCAGGTAAACCTCTTCTCCTTGTGACCTGGAATGGATTGAATCCTTCTTTGCCCTCGATTTTACTCAACTCTCACATGGACTCTGTCCCCGCTGAGCCCTCTAAATGGCTCTATCCGCCGTTCTCCGCCCTCCGGACCTCCGATGGAAAGATCTTCGCCCGTGGTTCTCAAGATGATAAATGTATAGCTATCCAGTATCTCGAAGCTATTCGGAACCTCCGAAGTCGGAATTTCATCCCTGACCGTACGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTTGATGGAGCTTCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGCTGAATGTTGGATTCATGATGGACGAAGGACAGGCGTCGCCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGCTGATAATTAGGGCTGAAGGAGCCCCTGGACATGGTTCTAGAATGTACGATAACAGTGCTATGGAGAATTTGATGAAGAGCGTTGAGATTATCAGTAAGTTTAGAGAGAGCCAATTCGATATGGTGAAGGCCGGTGAAACTGCTAATTCAGAAGTCATCTCTGTCAATCCTGTTTTCGTCAACGCCGGAATTCCCTCCCCCACTGGATTTGTGATGAACATGCAACCTTCGGAGGCGGAAGCCGGTTTCGATCTCAGAATACCGCCGACCGCCGACCCAGATGCCATTAGAAGAAGAATTGTGGAGGAATGGGCTCCAGCTCGACGGAACTTGACGTCTCAGATTATTGAGAAAGGACCCATAAGAGACTATTTGGGACGTCCATTAATGACGATGACTAACGATTCGAATCCATGGTGGTCTGTTTTCAAGACAGCCATCTCAGATGCTGGAGGTAAGCTTTCTAGGCCCGAAATCTTGGCTTCGACGACTGATGCACGCTTCATGAGACAGTTAGGCATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCATGAACACAACGAGTTCTTGAAGGACACCGTCTTCTTAAGGGGCATAAAGATTTATGAGTCCATAATCAGTGCCTTGAGTTCCTTTGTAGCGGATGCATCCCAAAAACAAACAAAACATTGGTCTTATACGCCATGTGCCTCTTTCACTTCATCTTCTTCCACACCACACCAACAAAAATTCATGGATCTCTGCGTTCTTGTTTCTATGCAGCGTACGGAAGCTGCTCCATTGCCAGCTGAAACCAAGAGGATAAAAGGGATAGAGATGGAGATTTGGAACTATGGGAAGAACCATTGTATCTTCCTTAAATCTTCTTCCAATCCGATTATGATTGATTTTGGTGGGTCTCGATTCAAAATTGAAAGCTTAGGGTCCCGTTAG

Protein sequence

MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADASQKQTKHWSYTPCASFTSSSSTPHQQKFMDLCVLVSMQRTEAAPLPAETKRIKGIEMEIWNYGKNHCIFLKSSSNPIMIDFGGSRFKIESLGSR
Homology
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BLAST of CmaCh04G023530 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.1e-93
Identity = 175/409 (42.79%), Postives = 246/409 (60.15%), Query Frame = 0 

Query: 34  DESDQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVT 93
           D   ++  ++ F+ YLRI T  P+PDY  A++FLE +A ++GL  + +E VPG  + ++T
Sbjct: 5   DPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVLT 64

Query: 94  WNGLNPSLPSILLNSHMDSVPAEPSKWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEA 153
           W G NPSLPSILLNSH D VP     W + PF A + S+G I+ARGSQD K ++IQYLEA
Sbjct: 65  WPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLEA 124

Query: 154 IRNLRSRNFIPDRTIHISYVPDEEIGGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEF 213
           +R L+S      RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D F
Sbjct: 125 VRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAF 184

Query: 214 RVFYADRSPWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-ETA 273
            VFY++RSPW + + + G PGH SR  +++A E L K +  I  FRE +   ++A     
Sbjct: 185 TVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHLK 244

Query: 274 NSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPAR 333
              V SVN   +  G+        N+ P+   A FD R+ P  D  A  +++      A 
Sbjct: 245 EGAVTSVNLTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQEAG 304

Query: 334 RNLTSQIIEKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARF 393
             +T +  +K         P MT T+DS+PWW+ F  A   A      PEI  + TD+R+
Sbjct: 305 EGVTFEFAQK------FTEPRMTPTDDSDPWWAAFSGACK-AMNLTLEPEIFPAATDSRY 364

Query: 394 MRQLGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSS 442
           +R +GIP LGFSPM+ TP+LLH+HNE L + +FLRG+ IY  ++SAL+S
Sbjct: 365 IRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

[Close]
BLAST of CmaCh04G023530 vs. ExPASy Swiss-Prot
Match: P37111 (Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 2.0e-92
Identity = 171/411 (41.61%), Postives = 248/411 (60.34%), Query Frame = 0 

Query: 38  QNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGL 97
           ++  ++ F+ YLRI+T  P+PDY +AV+FLE +A ++GL  + +E VPG  + ++TW G 
Sbjct: 9   EHPSVTLFRQYLRIRTVQPEPDYGAAVAFLEERARQLGLGCQKVEVVPGHVVTVLTWPGT 68

Query: 98  NPSLPSILLNSHMDSVPAEPSKWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNL 157
           NP+L SILLNSH D VP     W + PF   + +DG I+ RG+QD KC++IQYLEA+R L
Sbjct: 69  NPTLSSILLNSHTDVVPVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCVSIQYLEAVRRL 128

Query: 158 RSRNFIPDRTIHISYVPDEEIGGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFY 217
           +       RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG ASP D F VFY
Sbjct: 129 KVEGHHFPRTIHMTFVPDEEVGGHQGMELFVKRPEFQALRAGFALDEGLASPTDAFTVFY 188

Query: 218 ADRSPWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVI 277
           ++RSPW L + + G PGHGSR  +++A E L K +  I  FRE +   +++ +     V 
Sbjct: 189 SERSPWWLRVTSTGKPGHGSRFIEDTAAEKLHKVINSILAFREKEKQRLQSNQLKPGAVT 248

Query: 278 SVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTS 337
           SVN   +  G+        N+ P+   A FD R+ P  D  A   ++      A   +T 
Sbjct: 249 SVNLTMLEGGV------AYNVVPATMSACFDFRVAPDVDLKAFEEQLQSWCQAAGEGVTF 308

Query: 338 QIIEKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLG 397
           + ++K           +T T+DS+PWW+ F     D    L   EI  ++TDAR++R  G
Sbjct: 309 EFVQKWM------ETQVTSTDDSDPWWAAFSGVFKDMKLALEL-EICPASTDARYIRAAG 368

Query: 398 IPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADASQ 449
           +P LGFSPM++TP+LLH+H+E L + VFLRG+ IY  ++SAL+S  A  S+
Sbjct: 369 VPALGFSPMNHTPVLLHDHDERLHEAVFLRGVDIYTQLLSALASVPALPSE 406

[Close]
BLAST of CmaCh04G023530 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 2.9e-91
Identity = 173/408 (42.40%), Postives = 245/408 (60.05%), Query Frame = 0 

Query: 38  QNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGL 97
           ++  ++ F+ YLRI T  P+PDY SAV+FLE +A ++GL  + +E  PG  + ++TW G 
Sbjct: 9   EHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTWPGT 68

Query: 98  NPSLPSILLNSHMDSVPAEPSKWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNL 157
           NP L SILLNSH D VP     W + PF A + S+G I+ARG+QD K ++IQYLEA+R L
Sbjct: 69  NPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAVRRL 128

Query: 158 RSRNFIPDRTIHISYVPDEEIGGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFY 217
           +S      RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D F VFY
Sbjct: 129 KSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFY 188

Query: 218 ADRSPWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-ETANSEV 277
           ++RSPW + + + G PGH SR  +++A E L K V  I  FRE +   ++A        V
Sbjct: 189 SERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLKEGAV 248

Query: 278 ISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLT 337
            SVN   +  G+        N+ P+   A FD R+ P  D  A  +++      A   +T
Sbjct: 249 TSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAGEGVT 308

Query: 338 SQIIEKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL 397
            +  +K         P MT T+D++PWW+ F  A  +    L  PEI  + TD+R++R +
Sbjct: 309 FEFAQK------FTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRYIRAV 368

Query: 398 GIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVA 445
           GIP LGFSPM+ TP+LLH+HNE L + VFLRG+ IY  +++AL+S  A
Sbjct: 369 GIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALASVPA 403

[Close]
BLAST of CmaCh04G023530 vs. ExPASy Swiss-Prot
Match: Q5RFB0 (Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2)

HSP 1 Score: 335.1 bits (858), Expect = 1.5e-90
Identity = 172/413 (41.65%), Postives = 245/413 (59.32%), Query Frame = 0 

Query: 37  DQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNG 96
           +++  ++ F+ YLRI+T  P PDY +AV+F E +A ++GL  + +E  PG  + ++TW G
Sbjct: 8   EEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEERARQLGLGCQKVEVAPGYVVTVLTWPG 67

Query: 97  LNPSLPSILLNSHMDSVPAEPSKWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRN 156
            NP+L SILLNSH D VP     W + PF A + S+G I+ARG+QD KCI+IQYLEA+R 
Sbjct: 68  TNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCISIQYLEAVRR 127

Query: 157 LRSRNFIPDRTIHISYVPDEEIGGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVF 216
           L+       RTIH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VF
Sbjct: 128 LKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVF 187

Query: 217 YADRSPWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-ETANSE 276
           Y++RSPW + + + G PGH SR  +++A E L K V  I  FRE ++  +++        
Sbjct: 188 YSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVSSILAFREKEWQRLQSNPHLKEGS 247

Query: 277 VISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNL 336
           V SVN   +  G+        N+ P+   A FD R+ P  D  A   ++      A   +
Sbjct: 248 VTSVNLTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGV 307

Query: 337 TSQIIEKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQ 396
           T +  +K         P +T T+DSNPWW+ F     D    L  PEI+ + TD R++R 
Sbjct: 308 TLEFAQKWM------HPQVTPTDDSNPWWAAFSRVCKDMKLTL-EPEIMPAATDNRYIRA 367

Query: 397 LGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADASQ 449
           +GIP LGFSPM+ TP+LLH+H+E L + VFLRG+ IY  ++ AL+S  A  S+
Sbjct: 368 VGIPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPALPSE 407

[Close]
BLAST of CmaCh04G023530 vs. ExPASy Swiss-Prot
Match: Q6PTT0 (Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 3.2e-90
Identity = 168/409 (41.08%), Postives = 243/409 (59.41%), Query Frame = 0 

Query: 37  DQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNG 96
           +++  ++ F+ YLRI+T  P PDY +AV+F E  A ++GL  + +E  PG  + ++TW G
Sbjct: 8   EEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPG 67

Query: 97  LNPSLPSILLNSHMDSVPAEPSKWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRN 156
            NP+L SILLNSH D VP     W + PF A + S+G I+ RG+QD KC++IQYLEA++ 
Sbjct: 68  TNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQYLEAVKR 127

Query: 157 LRSRNFIPDRTIHISYVPDEEIGGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVF 216
           L+       RTIH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VF
Sbjct: 128 LKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPTDAFTVF 187

Query: 217 YADRSPWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-ETANSE 276
           Y++RSPW + + + G PGH SR  +++A E L K V  I  FRE ++  +++        
Sbjct: 188 YSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGS 247

Query: 277 VISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNL 336
           V SVN   +  G+        N+ P+   A FD R+ P  D  A   ++      A   +
Sbjct: 248 VTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEEQLQSWCQEAGEGV 307

Query: 337 TSQIIEKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQ 396
           T +  +K         P MT T+D++PWW+ F  A  +    L  PEI  + TD+R++R 
Sbjct: 308 TFEFAQK------FTEPRMTPTDDTDPWWAAFSGACKEMTLTL-EPEIFPAATDSRYIRA 367

Query: 397 LGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVA 445
           +GIP LGFSPM+ TP+LLH+HNE L + VFLRG+ IY  +++AL+S  A
Sbjct: 368 VGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALASVPA 403

[Close]
BLAST of CmaCh04G023530 vs. ExPASy TrEMBL
Match: A0A6J1JRT3 (aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.3e-259
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240
           FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY
Sbjct: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQ 449
           KDTVFLRGIKIYESIISALSSFVADASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]
BLAST of CmaCh04G023530 vs. ExPASy TrEMBL
Match: A0A6J1HLT6 (aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.3e-254
Identity = 439/448 (97.99%), Postives = 443/448 (98.88%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLF FPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIR+LRSRNFIPDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRAEG PGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRI EEWAPARRNLTSQIIEKGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQ 449
           KDTVFLRGIKIYESIISALSSFVADASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]
BLAST of CmaCh04G023530 vs. ExPASy TrEMBL
Match: A0A5A7URV3 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001820 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 6.1e-225
Identity = 379/449 (84.41%), Postives = 417/449 (92.87%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           M+NFRLH R FS+ ++L L  FPLSFSHSQY DDESDQ+SP+SRFQ YLRIKTAHPDPDY
Sbjct: 85  MSNFRLHHRFFSVTLYLLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDPDY 144

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL SQA EIGLHT++LEF PGKPLLLVTW G NPSLPS+LLNSHMDSVPAEPSKW
Sbjct: 145 ASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPSKW 204

Query: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180
           L+PPFSA+RTSDGKIFARGSQDDKCIAIQY+EAIRNLR+R+F+P RT+HISYVPDEEIGG
Sbjct: 205 LHPPFSAVRTSDGKIFARGSQDDKCIAIQYIEAIRNLRNRDFVPVRTVHISYVPDEEIGG 264

Query: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240
            DG +KFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW LII+A+G PGHGSRMY
Sbjct: 265 SDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSRMY 324

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIIS+FRESQF++VKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 325 DNSAMENLMKSVEIISRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 384

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLR+PPTADPDA+RRRI EEWAPA RN+T QIIEKGP+RDYLG PLMTMTNDS
Sbjct: 385 SEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTNDS 444

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNEFL
Sbjct: 445 NPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFL 504

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQK 450
            D+VF+RGIK+YES+IS+LSSF  D S +
Sbjct: 505 SDSVFIRGIKVYESLISSLSSFQEDVSSQ 533

[Close]
BLAST of CmaCh04G023530 vs. ExPASy TrEMBL
Match: A0A5D3BIQ8 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001910 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 6.1e-225
Identity = 379/449 (84.41%), Postives = 416/449 (92.65%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           M+NFRLH R FS+ ++L L  FPLSFSHSQY DDESDQ+SP+SRFQ YLRIKTAHPDPDY
Sbjct: 1   MSNFRLHHRFFSVTLYLLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL SQA EIGLHT++LEF PGKPLLLVTW G NPSLPS+LLNSHMDSVPAEPSKW
Sbjct: 61  ASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180
           L+PPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIRNLR+R+F+P RT+HISYVPDEEIGG
Sbjct: 121 LHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTVHISYVPDEEIGG 180

Query: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240
            DG +KFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW LII+A+G PGHGSRMY
Sbjct: 181 SDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQF++VKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIVSRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLR+PPTADPDA+RRRI EEWAPA RN+T QIIEKGP+RDYLG PLMTMTNDS
Sbjct: 301 SEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNEFL
Sbjct: 361 NPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQK 450
            D+VF+RGIK+YES+IS LSSF  D S +
Sbjct: 421 SDSVFIRGIKVYESLISTLSSFQEDVSSQ 449

[Close]
BLAST of CmaCh04G023530 vs. ExPASy TrEMBL
Match: A0A0A0LRI1 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005600 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.7e-222
Identity = 380/451 (84.26%), Postives = 418/451 (92.68%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFL--FPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDP 60
           M+NF LH RLFS+ ++L L L  FPLSFS SQY DDE DQ+SP+SRFQ YLRIKTAHPDP
Sbjct: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSQSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60

Query: 61  DYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPS 120
           DYASAV+FL SQA EIGLHT+ILEFV GKPLLLVTW GL+PSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGLDPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEI 180
           KW++PPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIRNLR+R+F+P RTIHISYVPDEEI
Sbjct: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180

Query: 181 GGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSR 240
           GG DGA+KFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW LII+A+G+PGHGSR
Sbjct: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNM 300
           MYDNSAMENLMKSVEI+++FRESQF++VKAGE ANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTN 360
           QPSEAEAGFDLR+PPTADPDA+RRRI EEWAPARRN+T QI+EKGPIRDYLG PLMTMTN
Sbjct: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDYLGSPLMTMTN 360

Query: 361 DSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNE 420
            SNPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNE
Sbjct: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420

Query: 421 FLKDTVFLRGIKIYESIISALSSFVADASQK 450
           FL D+VF+RGIK+YES+ISALSSF  D S +
Sbjct: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 451

[Close]
BLAST of CmaCh04G023530 vs. NCBI nr
Match: XP_022991055.1 (aminoacylase-1 [Cucurbita maxima])

HSP 1 Score: 905.2 bits (2338), Expect = 2.7e-259
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240
           FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY
Sbjct: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQ 449
           KDTVFLRGIKIYESIISALSSFVADASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]
BLAST of CmaCh04G023530 vs. NCBI nr
Match: KAG6602086.1 (Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 890.6 bits (2300), Expect = 6.9e-255
Identity = 440/452 (97.35%), Postives = 444/452 (98.23%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLF F LSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFSLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNF+PDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEG PGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL IPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQKQTK 453
           KDTVFLRGIKIYESIISALSSFVADAS   TK
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASLHPTK 452

[Close]
BLAST of CmaCh04G023530 vs. NCBI nr
Match: XP_022964785.1 (aminoacylase-1 [Cucurbita moschata])

HSP 1 Score: 888.6 bits (2295), Expect = 2.6e-254
Identity = 439/448 (97.99%), Postives = 443/448 (98.88%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLF FPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIR+LRSRNFIPDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRAEG PGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRI EEWAPARRNLTSQIIEKGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQ 449
           KDTVFLRGIKIYESIISALSSFVADASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]
BLAST of CmaCh04G023530 vs. NCBI nr
Match: KAG7032791.1 (Aminoacylase-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 888.3 bits (2294), Expect = 3.4e-254
Identity = 438/447 (97.99%), Postives = 442/447 (98.88%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLF F LSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFSLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNF+PDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEG PGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL IPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
           KDTVFLRGIKIYESIISALSSFVADAS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

[Close]
BLAST of CmaCh04G023530 vs. NCBI nr
Match: XP_023553812.1 (aminoacylase-1 [Cucurbita pepo subsp. pepo] >XP_023553821.1 aminoacylase-1 [Cucurbita pepo subsp. pepo] >XP_023553828.1 aminoacylase-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 878.2 bits (2268), Expect = 3.5e-251
Identity = 435/447 (97.32%), Postives = 439/447 (98.21%), Query Frame = 0 

Query: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAV LFLF FPLSFS S+Y DDESDQNS ISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVILFLFSFPLSFSDSKYVDDESDQNSSISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSA+ TSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNF+PDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVLTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEG PGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
           KDTVFLRGIKIYESIISALSSFVADAS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

[Close]
BLAST of CmaCh04G023530 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 636.7 bits (1641), Expect = 1.7e-182
Identity = 295/430 (68.60%), Postives = 360/430 (83.72%), Query Frame = 0 

Query: 19  LFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHT 78
           L +F + FS        S++++PI+RFQ YLR  TAHP+P+Y + +SFL +QA  IGL T
Sbjct: 10  LIIFSIIFS---LQSSSSEEDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIGLTT 69

Query: 79  KILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKWLYPPFSALRTSDGKIFAR 138
           K +EF+ GKP+LL+TW G NP+LPSIL NSH+DSVPAE  KW YPPFSA +T DG I+AR
Sbjct: 70  KTIEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHIYAR 129

Query: 139 GSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGGFDGASKFVLSKEFKELNV 198
           G+QDDKCI +QYLE+IRNL+SR F P RTIHISYVP+EEIGGFDG  KF  S EFK+LN+
Sbjct: 130 GAQDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKFAASSEFKDLNL 189

Query: 199 GFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEIISKF 258
           GF MDEGQA+PGDEFRVFYADR PW  +I+AEG PGHG+++YDNSAMENLMKSVE+IS+F
Sbjct: 190 GFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVELISRF 249

Query: 259 RESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPD 318
           RESQFD VKAG+ A SEVISVNPV++ AG P+ TGFVMNMQPSEAEAG+DLR+PP ADPD
Sbjct: 250 RESQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPMADPD 309

Query: 319 AIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKL 378
            +++RI EEWAP+ RN+T  I EKG +RD+LGRP+MT  NDSNPWWS+FK A+   GGKL
Sbjct: 310 VMKKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAMGGKL 369

Query: 379 SRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISA 438
           ++PEILASTTD+RF+R LGIP  GFSPM+NTPILLH+HNEFLKDTVF++GI++YES+ISA
Sbjct: 370 AKPEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYESVISA 429

Query: 439 LSSFVADASQ 449
           LSSF  +++Q
Sbjct: 430 LSSFEGESAQ 436

[Close]
BLAST of CmaCh04G023530 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 627.5 bits (1617), Expect = 1.0e-179
Identity = 296/430 (68.84%), Postives = 362/430 (84.19%), Query Frame = 0 

Query: 15  VFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEI 74
           +FLF F+F L  SHS+    E ++++PI+RFQ YLR  TAHP+P+Y + +SFL  QA  I
Sbjct: 10  LFLFSFIFSLQ-SHSK----EEEEDTPITRFQQYLRFNTAHPNPNYTAPISFLLDQARSI 69

Query: 75  GLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKWLYPPFSALRTSDGK 134
           GL ++ +EFVPGKP+LL+TW G N +L SIL NSH+DSVPAE  KW++PPFSA RT DG 
Sbjct: 70  GLTSRTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHRTIDGH 129

Query: 135 IFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGGFDGASKFVLSKEFK 194
           I+ARG+QDDKCI +QYLEAIRNL+SR+F P RTIHISYVP+EEIGGF G  KF  S EFK
Sbjct: 130 IYARGAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASSEFK 189

Query: 195 ELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEI 254
           +LN+GF+MDEGQASPGDEFRVFYA+R+PW L IRA G PGHG+++YDNSAMENLMKSVE+
Sbjct: 190 DLNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLMKSVEL 249

Query: 255 ISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPT 314
           IS+FRESQFD VKAG+ ANSEVISVNPV++ AG PS TGFVMNMQPSEAE G+DLR+PP 
Sbjct: 250 ISRFRESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRLPPM 309

Query: 315 ADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDA 374
           ADP A+++RI EEWAP+ RN+T  + ++G + D+LGRP+MT TND+NPWWS+FK A+   
Sbjct: 310 ADPVALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAVEAT 369

Query: 375 GGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYES 434
           GGKL++PEIL STTD+RF+R LGIPVLGFSPM NTPIL+H+HNEFLKDTVF++GI +YES
Sbjct: 370 GGKLAKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGIGVYES 429

Query: 435 IISALSSFVA 445
           +ISALSSF A
Sbjct: 430 VISALSSFEA 434

[Close]
BLAST of CmaCh04G023530 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 486.5 bits (1251), Expect = 2.8e-137
Identity = 221/408 (54.17%), Postives = 302/408 (74.02%), Query Frame = 0 

Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           +SRFQ YLRI T  P+P+Y  AV F+ SQA  + L ++ +EFV GKPLLL+ W G +P+L
Sbjct: 25  VSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSDPTL 84

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 161
           P+ LLNSH D VP E SKW + P  A     G I+ARGSQD KC+ +QYLEAIR L++  
Sbjct: 85  PAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQASG 144

Query: 162 FIPDRTIHISYVPDEEIGGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
           F P R++++S+VPDEEIGG DGA KF  S+ FK LN+  ++DEG  SP + +RVFY +RS
Sbjct: 145 FKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERS 204

Query: 222 PWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNP 281
           PW L+I+A+G PGHG+++YDNSAMENL+KS+E I +FR SQFD++KAG  A  +V+SVN 
Sbjct: 205 PWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGDVVSVNM 264

Query: 282 VFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIE 341
            F+ AG PSPTGFVMN+QPSEAEAGFD+R+PP+ D +A+ RR+VEEWAPA RN++ +  +
Sbjct: 265 AFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNMSFEFKQ 324

Query: 342 KGPIRDYLGRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVL 401
           K       G+  +T  +DSNPWW + + A+ +AGG+ S+PEI  ++TDAR+ R+ G+P  
Sbjct: 325 K-----LTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGVPAF 384

Query: 402 GFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADASQK 450
           GFSP+SNTP LLH+HNE+L    +L+GI++Y SII A +S+ + +  +
Sbjct: 385 GFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASYESKSGSR 427

[Close]
BLAST of CmaCh04G023530 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 486.5 bits (1251), Expect = 2.8e-137
Identity = 223/409 (54.52%), Postives = 303/409 (74.08%), Query Frame = 0 

Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           +SRFQ YLRI T  P+P+Y  AV F+ SQA  + L ++ +EFV GKPLLL+ W G +P+L
Sbjct: 25  VSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSDPTL 84

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 161
           P+ LLNSH D VP E SKW + P  A     G I+ARGSQD KC+ +QYLEAIR L++  
Sbjct: 85  PAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQASG 144

Query: 162 FIPDRTIHISYVPDEEIGGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
           F P R++++S+VPDEEIGG DGA KF  S+ FK LN+  ++DEG  SP + +RVFY +RS
Sbjct: 145 FKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERS 204

Query: 222 PWGLIIRAEGAPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNP 281
           PW L+I+A+G PGHG+++YDNSAMENL+KS+E I +FR SQFD++KAG  A  +V+SVN 
Sbjct: 205 PWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGDVVSVNM 264

Query: 282 VFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIE 341
            F+ AG PSPTGFVMN+QPSEAEAGFD+R+PP+ D +A+ RR+VEEWAPA RN++    E
Sbjct: 265 AFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNMS---FE 324

Query: 342 KGPIRDYL-GRPLMTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPV 401
            G  +  L G+  +T  +DSNPWW + + A+ +AGG+ S+PEI  ++TDAR+ R+ G+P 
Sbjct: 325 LGQFKQKLTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGVPA 384

Query: 402 LGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADASQK 450
            GFSP+SNTP LLH+HNE+L    +L+GI++Y SII A +S+ + +  +
Sbjct: 385 FGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASYESKSGSR 430

The following BLAST results are available for this feature:
BLAST of CmaCh04G023530 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q99JW21.1e-9342.79Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1[more]
P371112.0e-9241.61Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2[more]
Q6AYS72.9e-9142.40Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1[more]
Q5RFB01.5e-9041.65Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2[more]
Q6PTT03.2e-9041.08Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1[more]
BLAST of CmaCh04G023530 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JRT31.3e-259100.00aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1[more]
A0A6J1HLT61.3e-25497.99aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1[more]
A0A5A7URV36.1e-22584.41Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00182... [more]
A0A5D3BIQ86.1e-22584.41Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00191... [more]
A0A0A0LRI11.7e-22284.26M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005600 P... [more]
BLAST of CmaCh04G023530 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022991055.12.7e-259100.00aminoacylase-1 [Cucurbita maxima][more]
KAG6602086.16.9e-25597.35Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022964785.12.6e-25497.99aminoacylase-1 [Cucurbita moschata][more]
KAG7032791.13.4e-25497.99Aminoacylase-1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023553812.13.5e-25197.32aminoacylase-1 [Cucurbita pepo subsp. pepo] >XP_023553821.1 aminoacylase-1 [Cucu... [more]
BLAST of CmaCh04G023530 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G44820.11.7e-18268.60Peptidase M20/M25/M40 family protein [more]
AT1G44180.11.0e-17968.84Peptidase M20/M25/M40 family protein [more]
AT4G38220.12.8e-13754.17Peptidase M20/M25/M40 family protein [more]
AT4G38220.22.8e-13754.52Peptidase M20/M25/M40 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 221..326
e-value: 6.4E-7
score: 29.3
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 32..224
e-value: 1.9E-52
score: 179.6
NoneNo IPR availableGENE3D1.10.150.900coord: 361..442
e-value: 2.6E-28
score: 99.6
NoneNo IPR availableGENE3D3.30.70.360coord: 225..334
e-value: 7.3E-9
score: 37.4
NoneNo IPR availablePANTHERPTHR45892AMINOACYLASE-1coord: 25..445
NoneNo IPR availablePANTHERPTHR45892:SF3PUTATIVE-RELATEDcoord: 25..445
NoneNo IPR availableCDDcd05646M20_AcylaseI_likecoord: 39..440
e-value: 0.0
score: 632.77
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 37..440
IPR010159N-acyl-L-amino-acid amidohydrolaseTIGRFAMTIGR01880TIGR01880coord: 36..442
e-value: 6.6E-179
score: 592.7
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 105..437
e-value: 6.8E-32
score: 111.5
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 225..329

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G023530.1CmaCh04G023530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004046 aminoacylase activity
molecular_function GO:0016787 hydrolase activity