CmaCh04G023380 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G023380
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionbiotin--protein ligase 2-like
LocationCma_Chr04: 16249132 .. 16256404 (-)
RNA-Seq ExpressionCmaCh04G023380
SyntenyCmaCh04G023380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTTAACTAACCCACTTCGCTGTTTCAGCTGGCGAGGCTCCCGCTGCTTTCATTCCATCATAAGCTCTCCCTCTTTTAGGTCTCCGATCAATACAACTGCTTCAGCGCCCACCCCAGGTTTGTTTCTTGATTGTTTCGATATATCTAGAAGTAATTTTCCTGATATTTTTGGTTCATTATGTTGATTTGAGACGAATTTTACTTTGCTGAGGTTTGTCGATCTGTCTCATAAAGATTTATGATTCTTTTCTGAAAAGAAGTTGAATAGCCATGTGGGGTGAGGATTTAGAGGATTGAAATTGGTTTTCTTCTGGAAAACAGGGACTTCCAGTAAAGTTAGTGAAAAAGGGAGATTGTTCTCTCTATTTTCTGGTTTAGCAATGGAGGCTAGTTCAACCTGTGCGTTGGTTTTGAGTGGAAAAACAGAGGCTGAGAATGAAACTGCCAAATTGCTTAAGAGGAATGACACTTTGAAGCTCCCAGACGATATCGAAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTTTGAAGGAAAGTGGTTTTCAGATTGGTTCATATATGAATTCTCTTTCTACTGATACTTTTGGTAGATTCCTCATTTGGTGTTCACGGATTCCTTCAACTCAGGATGTAATTTCTCAGTAAGTGTATGAAGACTTTTTTTCGTTTTTTGCCACATTACTTTTTCGTTCATCGACGTGCAAGCTAACTATTAGTCTGCAACAGGAACTTCAGCGATCTCCCATTGGGAGCTGTATGTGTGGCTGATGTTCAGTTCAAGGGAAGAGGTGCGTGGTGCTAATAAGCGAGACTGGTAACATTGTTTGTTAGATATGGAATTCATCTTTCTAATGTAAATCATTATGTGGATTTAAATGAAGGTCGATCAAAGAATTTGTGGGAATCTCCCCCTGGTTGCCTAATGTTTTCCTTTACCATTCAAATGGAAGATGGCCGTATAGTTCCTCTACTACAGTATGTAATATCTCTAGCTATTACTGAGGCTGTAAAAGATATTTGCGACAAAAAGGTAACCCTTGTCGTGTTCTGGCTCATATTTTCAAATCTTATTCAAATCTTTGTTAAACCGTGGCAACTCTATTATAATCAGTCTCCTAGTTTAAAATTTTGATCAACAAGTGATGTTAATTTAATGGTGCCCTGATATGCTAAATTACCAGAACTTTAGAAGGGGCTAGAGAAAAACATATGGTCATGGAAGGAGGAAACAGTTTGTTGGAAAGAATCTGGAGGAACTTAAGGAATAGATAGGGTAGATAGGGTTTTAAGTCATGTTATGAGTATTATTTTGTAAATGGGGCTGCACTTGAGTTGAGATTAATACCTCTCATATTGCAGGGTTTACCTTACATTGATTTGAAAATAAAATGGCCAAATGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTGTGCACATCAACATATAGATCAAAGAAGTTCAACGTTACTGCTGGTAATTGTGTTTGTTTTCTTCCATCCAGGCCGTTTTCCCTTTCCAAATTGAAACTTAGTCTCCATACCATACCTTACACCAATTTGAAAGGCCTAGCGTTGTTGTGATATATTTTAATTGCTTTATGTTCTTCCAGGTATAGGATTAAATGTAGACAATGATAAACCAACCACATGCTTGAATGAAGCTCTTGCGAATTTATCCAGTACGCCTTACAAGTTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAGGACTGTATGATATCTTCATAAATCAAGGTATTCATATGCCAATCTAATGTCTTTCACATGATAAGACATTGTCACATACATGCACGAATTTAGAGCTTCTCCTTCAATCCAACCTTAACAATGCTCTGGAGTTGATTGTAAGTCATGAAAATTTATAGTAGCAGCTAATACCATTTGCAAAAAGAATTTGAAATTGGCCATCTTTCTCTTCTTTTTCCCCCATAGCTGAACATGTTGGTGTGAACATCATTCTATCACTTCATGCTGTTCGATGCCTTACGGTTTTGTGCATGCTATGCATTTTGCTTCTACATATGGCATAAAGGATAGGTGAACTTCAGATGAATGAGTATCCGTGAAGGAAATCTTCCCAAGTTTTCTAGTTACATTTCCTTCAAATCTGTAAACATCATAGCTGTATGTAATATTTCCACTGGAAGGAGAGGGAGCGAGAGATAAGATATTGTTCGTTTAGGATGTAGATTTTTAATTGTGTTCTCTCGTGAAAAAGCTAGAATTTTGTAGCACGTCATGTTTATCAGTCTCAAGTTTGTTCTGCTTCAGTTTCAAAATTTCCCAGGTGACCTCATTCACTCTAAATCTGTGTAATGCCATTTTGTTTTCATCATTTCTGAATCATTAGGGTTTCAGGCTCTTGAGGAACTCTACTGTCAGACATGGTTGCACAGGTAACTCATGATGATTGATCTTTTTCTTTATATATATATTATCAACATGTGCTCTGTTCCAACATTCTCACGATTATACTTAGTTTGCATTGTCTATACATTTGTTGTCATGGGAACCAAAAACATCCTGGAAATGGACTATGTTGTTTGATCTTTTTAGCAGTTAATTTCTTTAAGCTAGTAATACTTGCTTATTTTTCTGAATCTCCATCTTTACGTAAGTCATACGTCACTAGTTTCAATTGTTTTGTTGTGTACATATACTTATGGCATTTACTGCTGTGCGAGTTATACCGACTAGTTCTTACAATTTTTTGTTACATCTTTGTACGTAGTGGGCAAAGAGTTATTGTACAGGAAAAGAAAGAAGATCAAGTAGTGGAAAATGTAGTCACTATTCAGGTACGTTATATCTGAATGATAGCTAATTCTTTGCCTTTTCTAGTGTAACAGCCCAAACCCACCGCTAGTCGATATTATCCGCTTTGACTCGTTATATATCGCCGTCAGTTTCACGATTTTTAAAACACGTCTGCTAAGGAGAGGTTTCCACACTCTTATTCCTTGTTTCATTTCCCTCTCTAACTGATGTGGGATCACATCTAGTTTCTACGCAAATGCTGCAGACTTAATCCCACTACTCTTTCTGGCTCTTCACTGTGGCTGTTTATATCTTTCCGGTTCAGAAGAAATGACCCTGTCTCCTGCAGCCAATTGTCATGTAGTTATTGCTGTTAGATAATCCTATTCCCGAAGGGCGGTCTTGTTCAAATATTCAATATCAATTTCTTGTCCAACTTTGTACTGGAGAGTGCTAAATGCACTATGTTCTATGATTTTCATGGTTTTAGAAACAGGAACTGCTTTCATGATCACAAGTTTGCTTGTTTCTTTATACAGGGGTTGACACCTACAGGATATTTGCTAGCTATTGGAGATGACAACCAAATGTGCGAGCTCCATCCCGATGGAAATAGGTTTAAGTATTTGAATGAATTCTTAATATTGATTTAGTTAATGGACTATGTTGAGCTTTAATTGACTATATGTAATGGGTCAAAGCGGACAATATCTTCTAGTGGTGGGTTTGGGCTGTTACAAATGGTATCAGAGTTAGACACCGGGCGGTGTGCCAGCGAGGACGTTGGGCCCCAAGGGGGTGGATTGTGAGATCCCACATCGATTTAAGAGAGGAACAAGTGCCAGTAAGGACGCTAGCCCTAAAGGGGAGTGGATTGTGAGATCCCACATCAATTGAAGAGGAGAATGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCTAGACGCGCTTTAAAACCTTGAGGGAAAGTTCAGAAGGAAAAGCCCAAAGAGAACAATATTTGCTAGCAGTATCTGATACAATGTGATACGTTAACAGCAAGAGTACAACATCTATGTCTATGGCATATCAATTGCTTTGTGAAACTAACTCCCAAGATCGAGAGAATTCATTGTATGCAGTTTGTCCCAAACTGGATTTTCAAACGACAAATAGGAACACTTAATAAGTCGTCTGCTTCAACCTTCCACCAAAAATCTGGTCACAACATTCTAAATTCCCCCCCCCAAATTTGAGTCAAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

ATGTTCTTAACTAACCCACTTCGCTGTTTCAGCTGGCGAGGCTCCCGCTGCTTTCATTCCATCATAAGCTCTCCCTCTTTTAGGTCTCCGATCAATACAACTGCTTCAGCGCCCACCCCAGCAATGGAGGCTAGTTCAACCTGTGCGTTGGTTTTGAGTGGAAAAACAGAGGCTGAGAATGAAACTGCCAAATTGCTTAAGAGGAATGACACTTTGAAGCTCCCAGACGATATCGAAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTTTGAAGGAAAGTGGTTTTCAGATTGGTTCATATATGAATTCTCTTTCTACTGATACTTTTGGTAGATTCCTCATTTGGTGTTCACGGATTCCTTCAACTCAGGATGGTTTACCTTACATTGATTTGAAAATAAAATGGCCAAATGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTGTGCACATCAACATATAGATCAAAGAAGTTCAACGTTACTGCTGGTATAGGATTAAATGTAGACAATGATAAACCAACCACATGCTTGAATGAAGCTCTTGCGAATTTATCCAGTACGCCTTACAAGTTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAGGACTGTATGATATCTTCATAAATCAAGGGTTTCAGGCTCTTGAGGAACTCTACTGTCAGACATGGTTGCACAGTGGGCAAAGAGTTATTGTACAGGAAAAGAAAGAAGATCAAGTAGTGGAAAATGGGTTGACACCTACAGGATATTTGCTAGCTATTGGAGATGACAACCAAATGTGCGAGCTCCATCCCGATGGAAATAGCTTAGATTTCTTCAAAGGACTGATCAAGAGCAAACTGAGCTTATCTGAACTTTACGACTGTGAAATGAGTGTAAAGGAACAGAAGGACAAAAGATTCTTGTGGGAAGGCGCCATGTCAGACCCAGAAACTGGTGGAGCATGGGCCATGCCAGAACTGTGTTGGGTGGTGGGAGTGTCGTGGTGTGTAGAGCTTGAGGTAATCAAGAGCTGCAAAAGGGGAAGTCTTACTGTAAAACTAGGTTTGTTTTCACCTTACTGTAAAACTCATTATCTCATCGCAACGTTGTTGTCAGATCTAAACTATCCTTAGAACGCTCGTTTGTTCACAACCCTAAAAACTTTCGTTAATGCTTATTCCAATTCTAGAGATCGTTCGACTTTCAAAAAGTCTATTTTAATC

Coding sequence (CDS)

ATGTTCTTAACTAACCCACTTCGCTGTTTCAGCTGGCGAGGCTCCCGCTGCTTTCATTCCATCATAAGCTCTCCCTCTTTTAGGTCTCCGATCAATACAACTGCTTCAGCGCCCACCCCAGCAATGGAGGCTAGTTCAACCTGTGCGTTGGTTTTGAGTGGAAAAACAGAGGCTGAGAATGAAACTGCCAAATTGCTTAAGAGGAATGACACTTTGAAGCTCCCAGACGATATCGAAATTTCGGTTTCTTTGCACTCAGAAAGGGATAAATCTTTGAAGGAAAGTGGTTTTCAGATTGGTTCATATATGAATTCTCTTTCTACTGATACTTTTGGTAGATTCCTCATTTGGTGTTCACGGATTCCTTCAACTCAGGATGGTTTACCTTACATTGATTTGAAAATAAAATGGCCAAATGATCTTTATGTGAATGGCCTGAAAGTTGGAGGCATTCTGTGCACATCAACATATAGATCAAAGAAGTTCAACGTTACTGCTGGTATAGGATTAAATGTAGACAATGATAAACCAACCACATGCTTGAATGAAGCTCTTGCGAATTTATCCAGTACGCCTTACAAGTTCAGAAGAGAGGATATCTTAGCATTCTTCTTTAACAAATTTGAAGGACTGTATGATATCTTCATAAATCAAGGGTTTCAGGCTCTTGAGGAACTCTACTGTCAGACATGGTTGCACAGTGGGCAAAGAGTTATTGTACAGGAAAAGAAAGAAGATCAAGTAGTGGAAAATGGGTTGACACCTACAGGATATTTGCTAGCTATTGGAGATGACAACCAAATGTGCGAGCTCCATCCCGATGGAAATAGCTTAGATTTCTTCAAAGGACTGATCAAGAGCAAACTGAGCTTATCTGAACTTTACGACTGTGAAATGAGTGTAAAGGAACAGAAGGACAAAAGATTCTTGTGGGAAGGCGCCATGTCAGACCCAGAAACTGGTGGAGCATGGGCCATGCCAGAACTGTGTTGGGTGGTGGGAGTGTCGTGGTGTGTAGAGCTTGAGGTAATCAAGAGCTGCAAAAGGGGAAGTCTTACTGTAAAACTAGGTTTGTTTTCACCTTACTGTAAAACTCATTATCTCATCGCAACGTTGTTGTCAGATCTAAACTATCCTTAG

Protein sequence

MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPAMEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGSYMNSLSTDTFGRFLIWCSRIPSTQDGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNKFEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVENGLTPTGYLLAIGDDNQMCELHPDGNSLDFFKGLIKSKLSLSELYDCEMSVKEQKDKRFLWEGAMSDPETGGAWAMPELCWVVGVSWCVELEVIKSCKRGSLTVKLGLFSPYCKTHYLIATLLSDLNYP
Homology
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BLAST of CmaCh04G023380 vs. ExPASy Swiss-Prot
Match: Q9SL92 (Biotin--protein ligase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HCS1 PE=1 SV=2)

HSP 1 Score: 292.0 bits (746), Expect = 9.9e-78
Identity = 157/330 (47.58%), Postives = 199/330 (60.30%), Query Frame = 0 

Query: 41  AMEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLK--ESGFQ 100
           AME+ ++C+LVL GK+  E E AK LK  ++LKLPD+ ++S+ L SE    +K  ++ F 
Sbjct: 38  AMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVKDDDNSFN 97

Query: 101 IGSYMNSLSTDTFGRFLIWCSRIPSTQD-------------------------------- 160
           +  +MNS+ T  FGRFLIW  R+ ST D                                
Sbjct: 98  LSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNVWE 157

Query: 161 ------------------------------------------GLPYIDLKIKWPNDLYVN 220
                                                     GLPYID+KIKWPNDLYVN
Sbjct: 158 SPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYVN 217

Query: 221 GLKVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFF 280
           GLKVGGILCTSTYRSKKFNV+ G+GLNVDN +PTTCLN  L  ++      +RE+IL  F
Sbjct: 218 GLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILGAF 277

Query: 281 FNKFEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVEN-----GLTPTGYL 290
           F+KFE  +D+F++QGF++LEELY +TWLHS QRVIV++K EDQVV+N     GLT +GYL
Sbjct: 278 FHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQGLTSSGYL 337

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BLAST of CmaCh04G023380 vs. ExPASy Swiss-Prot
Match: F4I4W2 (Biotin--protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=HCS2 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 8.7e-74
Identity = 151/329 (45.90%), Postives = 192/329 (58.36%), Query Frame = 0 

Query: 42  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLK--ESGFQI 101
           M+  ++C+LVL GK+  E +TA  LK N+ LKLPD+ ++S+ L SE    ++  +S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 102 GSYMNSLSTDTFGRFLIWCSRIPSTQD--------------------------------- 161
             +MNS+ST  FGRFLIW   + ST D                                 
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 162 -----------------------------------------GLPYIDLKIKWPNDLYVNG 221
                                                    GL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 222 LKVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFF 281
           LK+GGILCTSTYRS+KF V+ G+GLNVDN++PTTCLN  L ++       +RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 282 NKFEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVEN-----GLTPTGYLL 290
            KFE  +D+F+ QGF++LEELY +TWLHSGQRVI +EK EDQVV+N     GLT +GYLL
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLL 300

[Close]
BLAST of CmaCh04G023380 vs. ExPASy Swiss-Prot
Match: Q920N2 (Biotin--protein ligase OS=Mus musculus OX=10090 GN=Hlcs PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.7e-21
Identity = 59/173 (34.10%), Postives = 89/173 (51.45%), Query Frame = 0 

Query: 121 IPSTQDGLPYIDLKIKWPNDLYVNGL-KVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTT 180
           IP  +D    I+L++KWPND+Y + L K+GG+L  ST   + F +  G G NV N  PT 
Sbjct: 552 IPGYED----INLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTI 611

Query: 181 CLNEALANLSSTP----YKFRREDILAFFFNKFEGLYDIFINQGFQALEELYCQTWLHSG 240
           C+N+ +   +          R + ++A      E L D F +QG   +  LY + W+H G
Sbjct: 612 CINDLIEEHNKQHGAGLKPLRADCLIARAVTVLEKLIDRFQDQGPDGVLPLYYKYWVHGG 671

Query: 241 QRVIVQEKKEDQVVENGLTPTGYLLAIGDDNQMCELHPDGNSLDFFKGLIKSK 289
           Q+V +   +  Q    GL  +G+L    +D  +  +HPDGNS D  + LI  K
Sbjct: 672 QQVRLGSTEGPQASIVGLDDSGFLQVHQEDGGVVTVHPDGNSFDMLRNLIVPK 720

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BLAST of CmaCh04G023380 vs. ExPASy Swiss-Prot
Match: P50747 (Biotin--protein ligase OS=Homo sapiens OX=9606 GN=HLCS PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.9e-21
Identity = 58/173 (33.53%), Postives = 92/173 (53.18%), Query Frame = 0 

Query: 121 IPSTQDGLPYIDLKIKWPNDLYVNGL-KVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTT 180
           IP  QD    I+L++KWPND+Y + L K+GG+L  ST   + F +  G G NV N  PT 
Sbjct: 556 IPEYQD----INLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTI 615

Query: 181 CLNEALANLS----STPYKFRREDILAFFFNKFEGLYDIFINQGFQALEELYCQTWLHSG 240
           C+N+ +   +    +     R + ++A      E L   F ++G  ++  LY + W+HSG
Sbjct: 616 CINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSG 675

Query: 241 QRVIVQEKKEDQVVENGLTPTGYLLAIGDDNQMCELHPDGNSLDFFKGLIKSK 289
           Q+V +   +  +V   GL  +G+L    +  ++  +HPDGNS D  + LI  K
Sbjct: 676 QQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPK 724

[Close]
BLAST of CmaCh04G023380 vs. ExPASy Swiss-Prot
Match: O14353 (Biotin--protein ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bpl1 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 9.8e-17
Identity = 60/185 (32.43%), Postives = 89/185 (48.11%), Query Frame = 0 

Query: 127 GLPYIDLKIKWPNDLYV------------NGLKVGGILCTSTYRSKKFNVTAGIGLNVDN 186
           G   I   IKWPND+YV              +K+ GI+ TS YR    ++  G G+NV N
Sbjct: 447 GYENIPAFIKWPNDVYVRVDKGGINFQGKQYMKLSGIIVTSNYRKNVLHLVVGCGINVSN 506

Query: 187 DKPTTCLNEALANL---SSTP--YKFRREDILAFFFNKFEGLYDIFINQGFQALEELYCQ 246
             PT  LN  +      S  P   KF  E +LA   N+F+  + + + +GF  +   Y Q
Sbjct: 507 LGPTVSLNTLVDEWNKNSDNPRLEKFSFEKLLASVLNQFDRYHRLLLEEGFSLILPEYYQ 566

Query: 247 TWLHSGQRVIVQEKKEDQVVENGLTPTGYLLA--IGDDNQ----MCELHPDGNSLDFFKG 289
            WLHS Q V +    +  +++   +  G+LLA  + ++N+    +  L PDGNS D  + 
Sbjct: 567 YWLHSNQTVNLASGGK-AIIQGITSDFGFLLAQLLNENNEPTTKVVHLQPDGNSFDLMRN 626

[Close]
BLAST of CmaCh04G023380 vs. ExPASy TrEMBL
Match: A0A6J1JK17 (biotin--protein ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487622 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.2e-149
Identity = 289/387 (74.68%), Postives = 289/387 (74.68%), Query Frame = 0 

Query: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTP-------------------A 60
           MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTP                   A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLFSGLA 60

Query: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120
           MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS
Sbjct: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNSLSTDTFGRFLIWCSRIPSTQD----------------------------------- 180
           YMNSLSTDTFGRFLIWCSRIPSTQD                                   
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 ---------------------------------------GLPYIDLKIKWPNDLYVNGLK 240
                                                  GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 290
           VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

[Close]
BLAST of CmaCh04G023380 vs. ExPASy TrEMBL
Match: A0A6J1GYI6 (biotin--protein ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458666 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 3.2e-148
Identity = 286/387 (73.90%), Postives = 288/387 (74.42%), Query Frame = 0 

Query: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTP-------------------A 60
           MFLTNPLRCFSWRGSRCFHSII+SPSFRSPINTTASAPTP                   A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120
           ME SSTC+LVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNSLSTDTFGRFLIWCSRIPSTQD----------------------------------- 180
           YMNSLSTDTFGRFLIWCSRIPSTQD                                   
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 ---------------------------------------GLPYIDLKIKWPNDLYVNGLK 240
                                                  GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKEGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 290
           VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

[Close]
BLAST of CmaCh04G023380 vs. ExPASy TrEMBL
Match: A0A6J1CKH6 (biotin--protein ligase 2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011837 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 2.3e-117
Identity = 240/368 (65.22%), Postives = 252/368 (68.48%), Query Frame = 0 

Query: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPAMEASSTCALVLSGKTEAEN 60
           M L N LR FS R SR F SI++S    S    + S P PAME SSTCALVLSGKT AEN
Sbjct: 1   MLLANQLRYFSRRDSRYFFSIVNSKVVASA--RSLSVPAPAMETSSTCALVLSGKTAAEN 60

Query: 61  ETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGSYMNSLSTDTFGRFLIWCSR 120
           ETAKLLKRN+TLKLPDD  +SV LHSE DK L+E+GFQI  YMNSLSTD FGRFLIWC R
Sbjct: 61  ETAKLLKRNNTLKLPDDTGLSVFLHSEADKHLEENGFQIDLYMNSLSTDAFGRFLIWCPR 120

Query: 121 IPSTQD------------------------------------------------------ 180
           IPSTQD                                                      
Sbjct: 121 IPSTQDVISHNFSDLPLGSVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQMEDGRIVPL 180

Query: 181 --------------------GLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVTA 240
                               GLPYIDLKIKWPNDLYVNGLK+GGILCTSTYRSKKFNV+A
Sbjct: 181 LQYVVSLAITEAIKDICDKKGLPYIDLKIKWPNDLYVNGLKLGGILCTSTYRSKKFNVSA 240

Query: 241 GIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNKFEGLYDIFINQGFQALEEL 290
           GIGLNVDNDKPTTCLN AL NLSSTP KFRREDILA FFNKFE L+DIFINQGFQALEEL
Sbjct: 241 GIGLNVDNDKPTTCLNAALKNLSSTPDKFRREDILASFFNKFERLHDIFINQGFQALEEL 300

[Close]
BLAST of CmaCh04G023380 vs. ExPASy TrEMBL
Match: A0A1S3BH31 (biotin--protein ligase 2-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC103489963 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 3.6e-115
Identity = 235/387 (60.72%), Postives = 256/387 (66.15%), Query Frame = 0 

Query: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPA------------------- 60
           M L N LR F  R SR   S+++S SF S     AS PTPA                   
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120
           M+AS TC+LVLSGKT AENETAKLLKRNDTLKLPDD EISV LHSE+DK L+E+GF+I  
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNSLSTDTFGRFLIWCSRIPSTQD----------------------------------- 180
           Y+N+LSTDTFGRFLIW  R+PSTQD                                   
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 ---------------------------------------GLPYIDLKIKWPNDLYVNGLK 240
                                                  GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 290
           VGG+LCTSTYR KKFNVTAGIGLNV+NDKP+TCLNEAL +LSSTPYKFR+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

[Close]
BLAST of CmaCh04G023380 vs. ExPASy TrEMBL
Match: A0A1S3BIK3 (biotin--protein ligase 2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103489963 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 3.1e-114
Identity = 235/395 (59.49%), Postives = 256/395 (64.81%), Query Frame = 0 

Query: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPA------------------- 60
           M L N LR F  R SR   S+++S SF S     AS PTPA                   
Sbjct: 1   MLLANQLRYFGRRDSRFVFSVLNSSSFWSLSRIVASPPTPAISINERERRRLFSLSSASG 60

Query: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120
           M+AS TC+LVLSGKT AENETAKLLKRNDTLKLPDD EISV LHSE+DK L+E+GF+I  
Sbjct: 61  MDASPTCSLVLSGKTAAENETAKLLKRNDTLKLPDDTEISVLLHSEKDKPLEENGFRIDL 120

Query: 121 YMNSLSTDTFGRFLIWCSRIPSTQD----------------------------------- 180
           Y+N+LSTDTFGRFLIW  R+PSTQD                                   
Sbjct: 121 YLNALSTDTFGRFLIWSPRVPSTQDVISHNFSNLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 ---------------------------------------GLPYIDLKIKWPNDLYVNGLK 240
                                                  GLPYIDLKIKWPNDLYVN LK
Sbjct: 181 GCLMFSFTIQMEDGRTVPLLQYVISLAITEAIKDICDKEGLPYIDLKIKWPNDLYVNDLK 240

Query: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 290
           VGG+LCTSTYR KKFNVTAGIGLNV+NDKP+TCLNEAL +LSSTPYKFR+EDILAFFFNK
Sbjct: 241 VGGVLCTSTYRLKKFNVTAGIGLNVENDKPSTCLNEALTDLSSTPYKFRKEDILAFFFNK 300

[Close]
BLAST of CmaCh04G023380 vs. NCBI nr
Match: XP_022990857.1 (biotin--protein ligase 2-like [Cucurbita maxima])

HSP 1 Score: 538.5 bits (1386), Expect = 4.6e-149
Identity = 289/387 (74.68%), Postives = 289/387 (74.68%), Query Frame = 0 

Query: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTP-------------------A 60
           MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTP                   A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLFSGLA 60

Query: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120
           MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS
Sbjct: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNSLSTDTFGRFLIWCSRIPSTQD----------------------------------- 180
           YMNSLSTDTFGRFLIWCSRIPSTQD                                   
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 ---------------------------------------GLPYIDLKIKWPNDLYVNGLK 240
                                                  GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 290
           VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

[Close]
BLAST of CmaCh04G023380 vs. NCBI nr
Match: XP_022957207.1 (biotin--protein ligase 2-like [Cucurbita moschata])

HSP 1 Score: 534.6 bits (1376), Expect = 6.7e-148
Identity = 286/387 (73.90%), Postives = 288/387 (74.42%), Query Frame = 0 

Query: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTP-------------------A 60
           MFLTNPLRCFSWRGSRCFHSII+SPSFRSPINTTASAPTP                   A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120
           ME SSTC+LVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNSLSTDTFGRFLIWCSRIPSTQD----------------------------------- 180
           YMNSLSTDTFGRFLIWCSRIPSTQD                                   
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 ---------------------------------------GLPYIDLKIKWPNDLYVNGLK 240
                                                  GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKEGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 290
           VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

[Close]
BLAST of CmaCh04G023380 vs. NCBI nr
Match: XP_023517017.1 (biotin--protein ligase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 534.3 bits (1375), Expect = 8.8e-148
Identity = 285/387 (73.64%), Postives = 288/387 (74.42%), Query Frame = 0 

Query: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTP-------------------A 60
           MFLTNPLRCFSWRGSRCFHSII+SPSFRSPINTTASAPTP                   A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120
           ME SSTC+LVLSGKTEAENETAKLLKRNDTLKLPDDIEIS+SLHSERDKSLKESGFQIGS
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISISLHSERDKSLKESGFQIGS 120

Query: 121 YMNSLSTDTFGRFLIWCSRIPSTQD----------------------------------- 180
           YMNSLSTDTFGRFLIWCSRIPSTQD                                   
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 ---------------------------------------GLPYIDLKIKWPNDLYVNGLK 240
                                                  GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVVSLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 290
           VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 300

[Close]
BLAST of CmaCh04G023380 vs. NCBI nr
Match: KAG6602073.1 (Serine/threonine-protein kinase MPS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 505.8 bits (1301), Expect = 3.3e-139
Identity = 272/376 (72.34%), Postives = 276/376 (73.40%), Query Frame = 0 

Query: 12   WRGSRCFHSIISSPSFRSPINTTASAPTP-------------------AMEASSTCALVL 71
            WRGSRCFHSII+SPSFRSPINTTASAPTP                   AME SSTC+LVL
Sbjct: 845  WRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLAMETSSTCSLVL 904

Query: 72   SGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGSYMNSLSTDTFG 131
            SGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGSYMNSLSTDTFG
Sbjct: 905  SGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGSYMNSLSTDTFG 964

Query: 132  RFLIWCSRIPSTQD---------------------------------------------- 191
            RFLIWCSRIPSTQD                                              
Sbjct: 965  RFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPPGCLMFSFTIQM 1024

Query: 192  ----------------------------GLPYIDLKIKWPNDLYVNGLKVGGILCTSTYR 251
                                        GLPYIDLKIKWPNDLYVNGLKVGGILCTSTYR
Sbjct: 1025 EDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLKVGGILCTSTYR 1084

Query: 252  SKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNKFEGLYDIFINQ 290
            SKKFNVTAGIGLN+DNDKPTTCLNEALANLSSTPYKFRREDIL+FFFNKFE LYDIFINQ
Sbjct: 1085 SKKFNVTAGIGLNLDNDKPTTCLNEALANLSSTPYKFRREDILSFFFNKFERLYDIFINQ 1144

[Close]
BLAST of CmaCh04G023380 vs. NCBI nr
Match: KAG7032770.1 (Biotin--protein ligase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 456.4 bits (1173), Expect = 2.3e-124
Identity = 246/347 (70.89%), Postives = 251/347 (72.33%), Query Frame = 0 

Query: 1   MFLTNPLRCFSWRGSRCFHSIISSPSFRSPINTTASAPTP-------------------A 60
           MFLTNPLRCFSWRGSRCFHSII+SPSFRSPINTTASAPTP                   A
Sbjct: 1   MFLTNPLRCFSWRGSRCFHSIINSPSFRSPINTTASAPTPGTSSKVSEKGRLFSLLSGLA 60

Query: 61  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120
           ME SSTC+LVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS
Sbjct: 61  METSSTCSLVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLKESGFQIGS 120

Query: 121 YMNSLSTDTFGRFLIWCSRIPSTQD----------------------------------- 180
           YMNSLSTDTFGRFLIWCSRIPSTQD                                   
Sbjct: 121 YMNSLSTDTFGRFLIWCSRIPSTQDVISQNFSDLPLGAVCVADVQFKGRGRSKNLWESPP 180

Query: 181 ---------------------------------------GLPYIDLKIKWPNDLYVNGLK 240
                                                  GLPYIDLKIKWPNDLYVNGLK
Sbjct: 181 GCLMFSFTIQMEDGRIVPLLQYVISLAITEAVKDICDKKGLPYIDLKIKWPNDLYVNGLK 240

Query: 241 VGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFFNK 255
           VGGILCTSTYRSKKFNVTAGIGLN+DNDKPTTCLNEALANLSSTPYKFRREDIL+FFFNK
Sbjct: 241 VGGILCTSTYRSKKFNVTAGIGLNLDNDKPTTCLNEALANLSSTPYKFRREDILSFFFNK 300

[Close]
BLAST of CmaCh04G023380 vs. TAIR 10
Match: AT2G25710.1 (holocarboxylase synthase 1 )

HSP 1 Score: 292.0 bits (746), Expect = 7.1e-79
Identity = 157/330 (47.58%), Postives = 199/330 (60.30%), Query Frame = 0 

Query: 41  AMEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLK--ESGFQ 100
           AME+ ++C+LVL GK+  E E AK LK  ++LKLPD+ ++S+ L SE    +K  ++ F 
Sbjct: 38  AMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVKDDDNSFN 97

Query: 101 IGSYMNSLSTDTFGRFLIWCSRIPSTQD-------------------------------- 160
           +  +MNS+ T  FGRFLIW  R+ ST D                                
Sbjct: 98  LSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNVWE 157

Query: 161 ------------------------------------------GLPYIDLKIKWPNDLYVN 220
                                                     GLPYID+KIKWPNDLYVN
Sbjct: 158 SPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYVN 217

Query: 221 GLKVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFF 280
           GLKVGGILCTSTYRSKKFNV+ G+GLNVDN +PTTCLN  L  ++      +RE+IL  F
Sbjct: 218 GLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILGAF 277

Query: 281 FNKFEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVEN-----GLTPTGYL 290
           F+KFE  +D+F++QGF++LEELY +TWLHS QRVIV++K EDQVV+N     GLT +GYL
Sbjct: 278 FHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQGLTSSGYL 337

[Close]
BLAST of CmaCh04G023380 vs. TAIR 10
Match: AT2G25710.2 (holocarboxylase synthase 1 )

HSP 1 Score: 292.0 bits (746), Expect = 7.1e-79
Identity = 157/330 (47.58%), Postives = 199/330 (60.30%), Query Frame = 0 

Query: 41  AMEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLK--ESGFQ 100
           AME+ ++C+LVL GK+  E E AK LK  ++LKLPD+ ++S+ L SE    +K  ++ F 
Sbjct: 38  AMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVKDDDNSFN 97

Query: 101 IGSYMNSLSTDTFGRFLIWCSRIPSTQD-------------------------------- 160
           +  +MNS+ T  FGRFLIW  R+ ST D                                
Sbjct: 98  LSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNVWE 157

Query: 161 ------------------------------------------GLPYIDLKIKWPNDLYVN 220
                                                     GLPYID+KIKWPNDLYVN
Sbjct: 158 SPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYVN 217

Query: 221 GLKVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFF 280
           GLKVGGILCTSTYRSKKFNV+ G+GLNVDN +PTTCLN  L  ++      +RE+IL  F
Sbjct: 218 GLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILGAF 277

Query: 281 FNKFEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVEN-----GLTPTGYL 290
           F+KFE  +D+F++QGF++LEELY +TWLHS QRVIV++K EDQVV+N     GLT +GYL
Sbjct: 278 FHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQGLTSSGYL 337

[Close]
BLAST of CmaCh04G023380 vs. TAIR 10
Match: AT1G37150.2 (holocarboxylase synthetase 2 )

HSP 1 Score: 278.9 bits (712), Expect = 6.2e-75
Identity = 151/329 (45.90%), Postives = 192/329 (58.36%), Query Frame = 0 

Query: 42  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLK--ESGFQI 101
           M+  ++C+LVL GK+  E +TA  LK N+ LKLPD+ ++S+ L SE    ++  +S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 102 GSYMNSLSTDTFGRFLIWCSRIPSTQD--------------------------------- 161
             +MNS+ST  FGRFLIW   + ST D                                 
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 162 -----------------------------------------GLPYIDLKIKWPNDLYVNG 221
                                                    GL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 222 LKVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFF 281
           LK+GGILCTSTYRS+KF V+ G+GLNVDN++PTTCLN  L ++       +RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

Query: 282 NKFEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKEDQVVEN-----GLTPTGYLL 290
            KFE  +D+F+ QGF++LEELY +TWLHSGQRVI +EK EDQVV+N     GLT +GYLL
Sbjct: 241 KKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNVVTIQGLTSSGYLL 300

[Close]
BLAST of CmaCh04G023380 vs. TAIR 10
Match: AT1G37150.4 (holocarboxylase synthetase 2 )

HSP 1 Score: 240.4 bits (612), Expect = 2.4e-63
Identity = 112/168 (66.67%), Postives = 135/168 (80.36%), Query Frame = 0 

Query: 127 GLPYIDLKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALA 186
           GL Y D+KIKWPNDLY+NGLK+GGILCTSTYRS+KF V+ G+GLNVDN++PTTCLN  L 
Sbjct: 119 GLSYNDVKIKWPNDLYLNGLKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLK 178

Query: 187 NLSSTPYKFRREDILAFFFNKFEGLYDIFINQGFQALEELYCQTWLHSGQRVIVQEKKED 246
           ++       +RE+IL  FF KFE  +D+F+ QGF++LEELY +TWLHSGQRVI +EK ED
Sbjct: 179 DVCPPSNLLKREEILGAFFKKFENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNED 238

Query: 247 QVVEN-----GLTPTGYLLAIGDDNQMCELHPDGNSLDFFKGLIKSKL 290
           QVV+N     GLT +GYLLAIGDDN M ELHPDGNS DFFKGL++ KL
Sbjct: 239 QVVQNVVTIQGLTSSGYLLAIGDDNVMYELHPDGNSFDFFKGLVRRKL 286

[Close]
BLAST of CmaCh04G023380 vs. TAIR 10
Match: AT1G37150.3 (holocarboxylase synthetase 2 )

HSP 1 Score: 223.0 bits (567), Expect = 4.0e-58
Identity = 122/289 (42.21%), Postives = 161/289 (55.71%), Query Frame = 0 

Query: 42  MEASSTCALVLSGKTEAENETAKLLKRNDTLKLPDDIEISVSLHSERDKSLK--ESGFQI 101
           M+  ++C+LVL GK+  E +TA  LK N+ LKLPD+ ++S+ L SE    ++  +S F +
Sbjct: 1   MDIDASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNL 60

Query: 102 GSYMNSLSTDTFGRFLIWCSRIPSTQD--------------------------------- 161
             +MNS+ST  FGRFLIW   + ST D                                 
Sbjct: 61  SLFMNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWES 120

Query: 162 -----------------------------------------GLPYIDLKIKWPNDLYVNG 221
                                                    GL Y D+KIKWPNDLY+NG
Sbjct: 121 PKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNG 180

Query: 222 LKVGGILCTSTYRSKKFNVTAGIGLNVDNDKPTTCLNEALANLSSTPYKFRREDILAFFF 255
           LK+GGILCTSTYRS+KF V+ G+GLNVDN++PTTCLN  L ++       +RE+IL  FF
Sbjct: 181 LKIGGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFF 240

The following BLAST results are available for this feature:
BLAST of CmaCh04G023380 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SL929.9e-7847.58Biotin--protein ligase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HCS1 ... [more]
F4I4W28.7e-7445.90Biotin--protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=HCS2 PE=2 SV=1[more]
Q920N21.7e-2134.10Biotin--protein ligase OS=Mus musculus OX=10090 GN=Hlcs PE=1 SV=1[more]
P507472.9e-2133.53Biotin--protein ligase OS=Homo sapiens OX=9606 GN=HLCS PE=1 SV=1[more]
O143539.8e-1732.43Biotin--protein ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX... [more]
BLAST of CmaCh04G023380 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JK172.2e-14974.68biotin--protein ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487622 PE=3 S... [more]
A0A6J1GYI63.2e-14873.90biotin--protein ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458666 PE=3... [more]
A0A6J1CKH62.3e-11765.22biotin--protein ligase 2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A1S3BH313.6e-11560.72biotin--protein ligase 2-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC103489963... [more]
A0A1S3BIK33.1e-11459.49biotin--protein ligase 2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103489963... [more]
BLAST of CmaCh04G023380 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022990857.14.6e-14974.68biotin--protein ligase 2-like [Cucurbita maxima][more]
XP_022957207.16.7e-14873.90biotin--protein ligase 2-like [Cucurbita moschata][more]
XP_023517017.18.8e-14873.64biotin--protein ligase 2-like [Cucurbita pepo subsp. pepo][more]
KAG6602073.13.3e-13972.34Serine/threonine-protein kinase MPS1, partial [Cucurbita argyrosperma subsp. sor... [more]
KAG7032770.12.3e-12470.89Biotin--protein ligase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrospe... [more]
BLAST of CmaCh04G023380 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G25710.17.1e-7947.58holocarboxylase synthase 1 [more]
AT2G25710.27.1e-7947.58holocarboxylase synthase 1 [more]
AT1G37150.26.2e-7545.90holocarboxylase synthetase 2 [more]
AT1G37150.42.4e-6366.67holocarboxylase synthetase 2 [more]
AT1G37150.34.0e-5842.21holocarboxylase synthetase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 116..231
e-value: 2.6E-21
score: 77.8
NoneNo IPR availablePANTHERPTHR12835BIOTIN PROTEIN LIGASEcoord: 131..288
NoneNo IPR availablePANTHERPTHR12835:SF5HOLOCARBOXYLASE SYNTHETASE (BIOTIN-(PROPRIONYL-COA-CARBOXYLASE (ATP-HYDROLYSING)) LIGASE)coord: 131..288
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 116..231
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPFAMPF03099BPL_LplA_LipBcoord: 123..171
e-value: 1.5E-11
score: 44.3
IPR003142Biotin protein ligase, C-terminalPFAMPF02237BPL_Ccoord: 233..283
e-value: 9.8E-6
score: 25.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G023380.1CmaCh04G023380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
molecular_function GO:0016874 ligase activity