CmaCh04G022580 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh04G022580 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 1.2e-226
Identity = 371/549 (67.58%), Postives = 453/549 (82.51%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98
           MEDLKPRP +S+PLTP+GFLERAAT+YG+  S++Y   +YTWS+TH RCL LASS++S G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158
           I+ GHVVSV++PN P MYELHFAVPMAGAILN +N RLDARTIS LL HSESKL+FVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 159 YCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQ 218
              LI +A+ALFP +   P LV + D    E    +S  +  +FF SY+++I +GD  F+
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMAD----ESESGNSSELGKEFFCSYKDLIDRGDPDFK 180

Query: 219 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFH 278
           W+ P SEWDP++LNYTSGTTSSPKGVVHCHRG+FI   DSL++WGVPK  VYLWTL MFH
Sbjct: 181 WVMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFH 240

Query: 279 INGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLD 338
            NGWS+ WG+AAVGGTNIC+RKFD+ +I+ +I+RH VTHMC APVVLNML+N+  +  L 
Sbjct: 241 ANGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLK 300

Query: 339 RPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARL 398
             V+I+T G+PPP+AVLFR ESLGF V+HGYGLTETAG+V+ C+WK EWN LPA  +ARL
Sbjct: 301 TTVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARL 360

Query: 399 KARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDE 458
           K+RQGV T     +DVVD  TG +VK+DG ++GE+V+RGGS+M+GY KD E TAKS+T +
Sbjct: 361 KSRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTAD 420

Query: 459 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDE 518
           GWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILY++P + EAAVVARPDE
Sbjct: 421 GWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDE 480

Query: 519 FWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYV 578
           FWGETPCAFVSL++GLT+KPTE+EI+EYCR KLPR+MVPKTVVF  ELPKTSTGK++K++
Sbjct: 481 FWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFI 540

Query: 579 LKEMAMSIG 588
           L++MA  +G
Sbjct: 541 LRDMARGMG 545

BLAST of CmaCh04G022580 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 7.9e-215
Identity = 362/551 (65.70%), Postives = 446/551 (80.94%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSS- 98
           ME+LKPR  NS PLTP+GFLERAAT+YG+  S++Y D +YTWS+TH RCL++AS + S  
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 99  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQ 158
           G+++G VVSV++PN P MYEL+FAVPMAG +LN INTRL+A TIS +L HSESKLVFVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 159 AYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYK- 218
               L+ DA++LFP  T  P LVLI D+   E   + +V  R  F   YE++++KGD K 
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGE---NLTVEDREHFVCCYEDLVEKGDDKT 180

Query: 219 FQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSM 278
           F+W+RPISEWDPIVLNYTSGTTSSPKGVVH HR VF+   DSL++WGVPK  VYLWTL M
Sbjct: 181 FKWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPM 240

Query: 279 FHINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRS 338
           FH NGW ++WGIAAVGGTNIC+R+FD  +IF+LIRRH VTHMCAAP+VLNML+NS +   
Sbjct: 241 FHANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEP 300

Query: 339 LDRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQA 398
           L  PV ++TGG+PPPAAVL R ESLGF ++HGYG+TE  G+V+ C+WK EWNRLPA  QA
Sbjct: 301 LPNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQA 360

Query: 399 RLKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSIT 458
           RLK+RQGVRTAA+  VDVVD  +G SVK+DG+++GEIV++G SIM+GY K+  ATAK I 
Sbjct: 361 RLKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIR 420

Query: 459 DEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARP 518
            +GWF TGD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY++P V+EAAVVA P
Sbjct: 421 ADGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACP 480

Query: 519 DEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKK 578
           DE+WGETP AFV+L++G+  +PTE+EI+EYCR KL  FMVPK VVF  +LPKTSTGKI+K
Sbjct: 481 DEYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQK 540

Query: 579 YVLKEMAMSIG 588
           + LKE+  ++G
Sbjct: 541 FTLKEIVKTMG 548

BLAST of CmaCh04G022580 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 6.3e-212
Identity = 360/550 (65.45%), Postives = 443/550 (80.55%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYG-DTTYTWSETHHRCLQLASSLSSS 98
           ME +KP   NS PLTPIGFLERAAT+YG+  SI+YG +T YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 99  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQ 158
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 159 AYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKF 218
               L  +A+++    T+PP LV I D    EE   + V+ R  F  +Y+++I +GD  F
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMF 278
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG+F+ + DSL++W VPKN VYLWTL +F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 279 HINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSL 338
           H NGWS+ WGIAAVGGTN+C+RKFDA LI+ LIR H VTHMC APVVLNML+ + + + L
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 339 DRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQAR 398
           +RPV ILT G+PPPAAVL R ES+GF ++HGYGLTETAG+ + C+WK +WNRLPA  +AR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 399 LKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITD 458
           LKARQGVRT     +DVVD ++G+SV+++G ++GEIV+RG SIM+GY KD   T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALKN 420

Query: 459 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPD 518
            GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYTNP VNE AVVARPD
Sbjct: 421 -GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 519 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKY 578
            FWGETPCAFVSL+ GLT++PTE E+IEYCR K+P++MVPKTV FV ELPKTSTGK+ K+
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 579 VLKEMAMSIG 588
           VL+E+A  +G
Sbjct: 541 VLREIAKKMG 545

BLAST of CmaCh04G022580 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.9e-208
Identity = 353/552 (63.95%), Postives = 443/552 (80.25%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYG-DTTYTWSETHHRCLQLASSLSSS 98
           ME++KP   NS PLTPIGFLERAAT+YG+  SI+YG +T YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 99  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQ 158
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 159 AYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKF 218
               L  +A+++    T+PP LV+I D    EE   + V+    F  +Y+++I++GD  F
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMF 278
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG+F+ + DSL++W VPKN VYLWTL +F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 279 HINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSL 338
           H NGW+  WGIAAVGGTN+C+RKFDA LI+ LIR H VTHMC APVVLNML+ + +++ L
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 339 DRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQAR 398
           + PV ILT GSPPPA VL R ES+GF ++HGYGLTETAG+++ C+WK +WN LPA  +AR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 399 LKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITD 458
           LKARQGVRT     +DVVD ++G SV+++G ++GEIV+RG S+M+GY KD   T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALKN 420

Query: 459 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPD 518
            GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYT P VNE AVVARPD
Sbjct: 421 -GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 519 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKY 578
           EFWGETPCAFVSL+ G + KPTEEE++EYCR K+P++MVPKTV F+ ELPK+STGK+ K+
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 579 VLKEMAMSIGPK 590
           VL+++A  +G K
Sbjct: 541 VLRDIAKKMGDK 547

BLAST of CmaCh04G022580 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 2.0e-202
Identity = 353/550 (64.18%), Postives = 431/550 (78.36%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTT-YTWSETHHRCLQLASSLSSS 98
           MEDLKP   NS PLT +GFLERAAT+YG+  SI+YG++T YTW ET+HRCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 99  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQ 158
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 159 AYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKF 218
            Y  L  +A+ +     NPP LVLI ++   EE   + V+ R  F   Y ++I +G+  F
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANE--EEEEGGAEVTERSKFCYLYSDLITRGNPDF 180

Query: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMF 278
           +WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRG+F+   DSL +W VPK  VYLWTL +F
Sbjct: 181 KWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIF 240

Query: 279 HINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLD-NRS 338
           H NGW++ WGIAAVGGTN+C+RK  A  I+ LIR H VTHM  AP+VL +L+ S + ++ 
Sbjct: 241 HANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQP 300

Query: 339 LDRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQA 398
           L  PV  LT GS PPA VL R ESLGF V+HGYGLTETAG+++ C+WK  WNRLPA  QA
Sbjct: 301 LKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQA 360

Query: 399 RLKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSIT 458
           +LK+RQGVRT   + +DVVD ++G+SV++DG ++GEIV+RG SIM+GY K+   T  S  
Sbjct: 361 QLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSFK 420

Query: 459 DEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARP 518
           + GWF TGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE++LYTNP VNEAAVVARP
Sbjct: 421 N-GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARP 480

Query: 519 DEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKK 578
           DEFWGETPCAFVSL+ GLTRKPT++EIIEYC+ K+PR+M PKTV F+ ELPKTSTGKI K
Sbjct: 481 DEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIK 540

Query: 579 YVLKEMAMSI 587
            +LKE+A ++
Sbjct: 541 SLLKEIAKNM 544

BLAST of CmaCh04G022580 vs. ExPASy TrEMBL
Match: A0A6J1JS99 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487376 PE=4 SV=1)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 593/593 (100.00%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MYSNLTLWRPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLER 60
           MYSNLTLWRPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLER
Sbjct: 1   MYSNLTLWRPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLER 60

Query: 61  AATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120
           AATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF
Sbjct: 61  AATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120

Query: 121 AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLV 180
           AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLV
Sbjct: 121 AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLV 180

Query: 181 LIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTSS 240
           LIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTSS
Sbjct: 181 LIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTSS 240

Query: 241 PKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIRK 300
           PKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIRK
Sbjct: 241 PKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIRK 300

Query: 301 FDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIES 360
           FDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIES
Sbjct: 301 FDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIES 360

Query: 361 LGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKTG 420
           LGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKTG
Sbjct: 361 LGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKTG 420

Query: 421 KSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIKDR 480
           KSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIKDR
Sbjct: 421 KSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIKDR 480

Query: 481 SKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKPTE 540
           SKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKPTE
Sbjct: 481 SKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKPTE 540

Query: 541 EEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIGPKPSRL 594
           EEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIGPKPSRL
Sbjct: 541 EEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIGPKPSRL 593

BLAST of CmaCh04G022580 vs. ExPASy TrEMBL
Match: A0A6J1HFJ6 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111463113 PE=4 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 566/594 (95.29%), Postives = 576/594 (96.97%), Query Frame = 0

Query: 1   MYSNLTLWRPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLER 60
           MYS LTLWR IKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPR PNSAPLTPIGFLER
Sbjct: 1   MYSTLTLWRSIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRTPNSAPLTPIGFLER 60

Query: 61  AATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120
           AATIYG+ PSIIYG TTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF
Sbjct: 61  AATIYGDSPSIIYGHTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120

Query: 121 AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLV 180
           AVPMAGAILNTINTRLDARTISTLL HSESKLVFV QAYCALISDALALFP ETNPPSLV
Sbjct: 121 AVPMAGAILNTINTRLDARTISTLLSHSESKLVFVHQAYCALISDALALFPLETNPPSLV 180

Query: 181 LIDDD-AAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTS 240
           LIDDD AAAEESLSSSVSIRIDF DSYEEMIQKGD KFQWIRPISEWDPIVLNYTSGTTS
Sbjct: 181 LIDDDAAAAEESLSSSVSIRIDFVDSYEEMIQKGDSKFQWIRPISEWDPIVLNYTSGTTS 240

Query: 241 SPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIR 300
           SPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTL MFHINGWSFSWGIAAVGGTN+CIR
Sbjct: 241 SPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFHINGWSFSWGIAAVGGTNVCIR 300

Query: 301 KFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIE 360
           KFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGG+PPPAAVLFRIE
Sbjct: 301 KFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGAPPPAAVLFRIE 360

Query: 361 SLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKT 420
           SLGFDVTHGYGLTETAGMVICCSWKTEWNRLPA  QARLKARQGVRTAAVAAVDVVD +T
Sbjct: 361 SLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARLKARQGVRTAAVAAVDVVDLET 420

Query: 421 GKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIKD 480
           GKSVKQDGVSIGEIVV GGSIMVGYWKDREATAKS+TDEGWFLTGDVGVMHPDGYLEIKD
Sbjct: 421 GKSVKQDGVSIGEIVVSGGSIMVGYWKDREATAKSMTDEGWFLTGDVGVMHPDGYLEIKD 480

Query: 481 RSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKPT 540
           RSKDVIISGGENLSSVEVES+LYTNP+VNEAAVVARPDEFWGETPCAFVSLREGLTRKPT
Sbjct: 481 RSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARPDEFWGETPCAFVSLREGLTRKPT 540

Query: 541 EEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIGPKPSRL 594
           EEEII+YCRGKLPRFMVPKTVVF+AELPKTSTGKIKKYVLKE+AMSIG K SRL
Sbjct: 541 EEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYVLKEIAMSIGSKSSRL 594

BLAST of CmaCh04G022580 vs. ExPASy TrEMBL
Match: A0A6J1JKT9 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487797 PE=4 SV=1)

HSP 1 Score: 948.3 bits (2450), Expect = 1.5e-272
Identity = 469/579 (81.00%), Postives = 512/579 (88.43%), Query Frame = 0

Query: 9   RPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLERAATIYGNF 68
           +P  L F +L+ +K S HY   T SSK ISMEDLKPRP NS+PLTP+GFLERAATIYG+ 
Sbjct: 44  KPTSLMFTNLS-KKASKHY---TISSKSISMEDLKPRPANSSPLTPLGFLERAATIYGDS 103

Query: 69  PSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHFAVPMAGAI 128
           PSIIYGDTTYTWS+TH RCLQLASSLSS GIQRGH+VSVISPNTPPMYELHFAVPMAGAI
Sbjct: 104 PSIIYGDTTYTWSDTHCRCLQLASSLSSMGIQRGHIVSVISPNTPPMYELHFAVPMAGAI 163

Query: 129 LNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLVLIDDDAAA 188
           LNTINTRLDARTIS LL HSESKL+FVDQA+CALI DALALFPPET  P LVLI DDAAA
Sbjct: 164 LNTINTRLDARTISILLTHSESKLIFVDQAFCALIHDALALFPPETKRPMLVLITDDAAA 223

Query: 189 EESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTSSPKGVVHCH 248
           EES SS + I  DF DSYEEMI KGD  F+WIRP+SEWDPIVLNYTSGTTSSPKGVV CH
Sbjct: 224 EESSSSCIMIHDDFVDSYEEMILKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCH 283

Query: 249 RGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIRKFDAALIFS 308
           RG+FI   D LLEW V K SVYLWTL MFH NGWS +WG+AAVGGTNICIRKFDAALI++
Sbjct: 284 RGIFIITLDVLLEWEVQKQSVYLWTLPMFHGNGWSLTWGMAAVGGTNICIRKFDAALIYT 343

Query: 309 LIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIESLGFDVTHG 368
           LIRRHHVTH+C APVVLNMLTNS  NR  D PVKILT G+PPP+ VL R ESLGFDVTHG
Sbjct: 344 LIRRHHVTHLCGAPVVLNMLTNSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHG 403

Query: 369 YGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKTGKSVKQDGV 428
           YGLTETAG V+ CSWK EWN+LP   +ARLK+RQGVRT A+ AVDVVD KTGKSVKQDGV
Sbjct: 404 YGLTETAGPVVFCSWKAEWNQLPTKERARLKSRQGVRTVAMTAVDVVDQKTGKSVKQDGV 463

Query: 429 SIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIKDRSKDVIISG 488
           SIGEIV++GGSIM+GY KDR+AT KS+T+EGWF TGDVGVMHPDGYLEIKDRSKDVIISG
Sbjct: 464 SIGEIVLKGGSIMLGYLKDRDATTKSMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISG 523

Query: 489 GENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKPTEEEIIEYCR 548
           GENLSSVEVES+LY NP+VNEAAVVARPDEFWGETPCAFVS+REGLT+KPTEEEIIE+CR
Sbjct: 524 GENLSSVEVESVLYMNPSVNEAAVVARPDEFWGETPCAFVSIREGLTQKPTEEEIIEHCR 583

Query: 549 GKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIG 588
           GKLPRFMVPKTVVF+ ELPKTSTGKI+KY+L+EMAMS+G
Sbjct: 584 GKLPRFMVPKTVVFMTELPKTSTGKIQKYLLREMAMSMG 618

BLAST of CmaCh04G022580 vs. ExPASy TrEMBL
Match: A0A6J1E391 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430182 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.2e-266
Identity = 465/622 (74.76%), Postives = 512/622 (82.32%), Query Frame = 0

Query: 1   MYSNLTLWRPIKLTFQDLA-----------------------------------RRKQSS 60
           M S    WRP+KLTF+DL                                     +K S 
Sbjct: 1   MCSTWFRWRPVKLTFKDLTDSALVCNDYNSNFRRSTLVPIHEAKRSTYLIFTNISKKASK 60

Query: 61  HYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHH 120
           H+     SSK IS +DLKPRP NS+PLTP+GFLERAATIYG+ PSIIYGDTTYTWS+TH 
Sbjct: 61  HF---VISSKSISTQDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHR 120

Query: 121 RCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLL 180
           RCLQLASSLSS GIQRGH+VSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTIS LL
Sbjct: 121 RCLQLASSLSSIGIQRGHIVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILL 180

Query: 181 FHSESKLVFVDQAYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDS 240
            HSESKL+FVDQA+CALI DALALFPPET  P LVLI DDAAAEES SS + I  DF DS
Sbjct: 181 SHSESKLIFVDQAFCALIQDALALFPPETKRPMLVLIADDAAAEESSSSCIMIDNDFVDS 240

Query: 241 YEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVP 300
           YEEMI+KGD  F+WIRP+SEWDPIVLNYTSGTTSSPKGVV CHRG+FI   D LLEW V 
Sbjct: 241 YEEMIRKGDSDFRWIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVT 300

Query: 301 KNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVL 360
           K SVYLWTL MFH NGWS +WG+AAVGGTNIC+RKFDAALI++LIRRHHVTH+C APVVL
Sbjct: 301 KQSVYLWTLPMFHGNGWSLTWGMAAVGGTNICVRKFDAALIYTLIRRHHVTHLCGAPVVL 360

Query: 361 NMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKT 420
           NMLTNS  NR  D PVKILT G+PPP+ VL R ESLGFDVTHGYGLTETAG V+ CSWK 
Sbjct: 361 NMLTNSPTNRPFDWPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKA 420

Query: 421 EWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYW 480
           EWN+L    +ARLK+RQGVRT A+ AVDVVD KTG+SVK+DGVSIGEIV+RGGSIM+GY 
Sbjct: 421 EWNQLSTTERARLKSRQGVRTVAMTAVDVVDQKTGESVKRDGVSIGEIVLRGGSIMLGYL 480

Query: 481 KDREATAKSITDEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNP 540
           KDR+AT KS+TDEGWF +GDVGVMHPDGYLEIKDR KDVIISGGENLSSVEVES+LY NP
Sbjct: 481 KDRDATTKSMTDEGWFFSGDVGVMHPDGYLEIKDRLKDVIISGGENLSSVEVESVLYMNP 540

Query: 541 TVNEAAVVARPDEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAE 588
           +VNEAAVVARPDEFWGETPCAFVSLREGL +KPTE+EIIE+CR KLPRFMVPKTVVF+ E
Sbjct: 541 SVNEAAVVARPDEFWGETPCAFVSLREGLAQKPTEKEIIEHCREKLPRFMVPKTVVFMTE 600

BLAST of CmaCh04G022580 vs. ExPASy TrEMBL
Match: A0A6J1JUG4 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487609 PE=4 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 6.6e-265
Identity = 451/550 (82.00%), Postives = 494/550 (89.82%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98
           ME LKPRP NS+PLTPIGFLERAATIY + PSIIYG+TTYTWS+TH RCLQLASSLSS G
Sbjct: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158
           IQRGHVVSVISPNTPPMYELHFAVPM GAILNTINTRLDARTIS LL HSESKL+FVD+A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120

Query: 159 YCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQ 218
           +C LI +AL LFPPE N P LVL+ DDAAAEES SSSV I  +F DSYE MI+KGD +FQ
Sbjct: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEES-SSSVLIEGNFVDSYEGMIRKGDSEFQ 180

Query: 219 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFH 278
           WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRG FI   D LLEWGVPK SVYLWTL MFH
Sbjct: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240

Query: 279 INGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLD 338
            NGWSFSWG+AAVGGTNICIRKFDA L++SLIRRHHVTH+C APVVLNMLTNS D R LD
Sbjct: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300

Query: 339 RPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARL 398
            PVKILT G+P PA +LFRIESLGFDVTHGYGLTET G V+CCSWK EW++LPA+ +ARL
Sbjct: 301 WPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360

Query: 399 KARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDE 458
           K+RQGVRT A  AVDVVD +TG+SV +DGVS+GEIV+R GS+M+GY+KD EATAKS+T++
Sbjct: 361 KSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTED 420

Query: 459 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDE 518
           GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NP+VNEAAVVARP E
Sbjct: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHE 480

Query: 519 FWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYV 578
           FWGETPCAFVSLREGLT++PTEEEIIEYCRGKLPRFM PKTVVF+ ELPKTSTGKI+KY+
Sbjct: 481 FWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540

Query: 579 LKEMAMSIGP 589
           LKEMAMS+GP
Sbjct: 541 LKEMAMSMGP 549

BLAST of CmaCh04G022580 vs. NCBI nr
Match: XP_022990524.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 593/593 (100.00%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MYSNLTLWRPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLER 60
           MYSNLTLWRPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLER
Sbjct: 1   MYSNLTLWRPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLER 60

Query: 61  AATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120
           AATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF
Sbjct: 61  AATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120

Query: 121 AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLV 180
           AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLV
Sbjct: 121 AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLV 180

Query: 181 LIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTSS 240
           LIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTSS
Sbjct: 181 LIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTSS 240

Query: 241 PKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIRK 300
           PKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIRK
Sbjct: 241 PKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIRK 300

Query: 301 FDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIES 360
           FDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIES
Sbjct: 301 FDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIES 360

Query: 361 LGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKTG 420
           LGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKTG
Sbjct: 361 LGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKTG 420

Query: 421 KSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIKDR 480
           KSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIKDR
Sbjct: 421 KSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIKDR 480

Query: 481 SKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKPTE 540
           SKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKPTE
Sbjct: 481 SKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKPTE 540

Query: 541 EEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIGPKPSRL 594
           EEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIGPKPSRL
Sbjct: 541 EEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIGPKPSRL 593

BLAST of CmaCh04G022580 vs. NCBI nr
Match: XP_023554638.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 564/595 (94.79%), Postives = 578/595 (97.14%), Query Frame = 0

Query: 1   MYSNLTLWRPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLER 60
           MYS L+LW PIKLTFQ +AR+KQSSHYRALTKSSK ISMEDLKPR PNS+PLTPIGFLER
Sbjct: 1   MYSTLSLWSPIKLTFQVVARQKQSSHYRALTKSSKSISMEDLKPRTPNSSPLTPIGFLER 60

Query: 61  AATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120
           AATIYG+ PSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF
Sbjct: 61  AATIYGDSPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120

Query: 121 AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLV 180
           AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALI DALALFPPETNPPSLV
Sbjct: 121 AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALIFDALALFPPETNPPSLV 180

Query: 181 LIDDDAAA--EESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTT 240
           LIDDDAAA  EESLSSSVSIRIDF DSYEEMIQKGD KFQWIRPISEWDPIVLNYTSGTT
Sbjct: 181 LIDDDAAAAEEESLSSSVSIRIDFVDSYEEMIQKGDSKFQWIRPISEWDPIVLNYTSGTT 240

Query: 241 SSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICI 300
           SSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTL MFHINGWSFSWGIAAVGGTN+CI
Sbjct: 241 SSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFHINGWSFSWGIAAVGGTNVCI 300

Query: 301 RKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRI 360
           RKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLD+R LDRPVKILTGG+P PAAVLFRI
Sbjct: 301 RKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDDRPLDRPVKILTGGAPLPAAVLFRI 360

Query: 361 ESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSK 420
           ESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPA  QARLKARQGVRTAAVAAVDVVD +
Sbjct: 361 ESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARLKARQGVRTAAVAAVDVVDLE 420

Query: 421 TGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIK 480
           TGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKS+TDEGWFLTGDVGVMHPDGYLEIK
Sbjct: 421 TGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDEGWFLTGDVGVMHPDGYLEIK 480

Query: 481 DRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKP 540
           DRSKDVIISGGENLSSVEVES+LYTNP+VNEAAVVARPDEFWGETPCAFVSLREGLTRKP
Sbjct: 481 DRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARPDEFWGETPCAFVSLREGLTRKP 540

Query: 541 TEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIGPKPSRL 594
           TEEEIIEYCRGKLPRFMVPKTVVF+AELPKTSTGKIKKYVLKE+AMSIGPKPSRL
Sbjct: 541 TEEEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYVLKEIAMSIGPKPSRL 595

BLAST of CmaCh04G022580 vs. NCBI nr
Match: XP_022962703.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 566/594 (95.29%), Postives = 576/594 (96.97%), Query Frame = 0

Query: 1   MYSNLTLWRPIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRPPNSAPLTPIGFLER 60
           MYS LTLWR IKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPR PNSAPLTPIGFLER
Sbjct: 1   MYSTLTLWRSIKLTFQDLARRKQSSHYRALTKSSKPISMEDLKPRTPNSAPLTPIGFLER 60

Query: 61  AATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120
           AATIYG+ PSIIYG TTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF
Sbjct: 61  AATIYGDSPSIIYGHTTYTWSETHHRCLQLASSLSSSGIQRGHVVSVISPNTPPMYELHF 120

Query: 121 AVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQAYCALISDALALFPPETNPPSLV 180
           AVPMAGAILNTINTRLDARTISTLL HSESKLVFV QAYCALISDALALFP ETNPPSLV
Sbjct: 121 AVPMAGAILNTINTRLDARTISTLLSHSESKLVFVHQAYCALISDALALFPLETNPPSLV 180

Query: 181 LIDDD-AAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQWIRPISEWDPIVLNYTSGTTS 240
           LIDDD AAAEESLSSSVSIRIDF DSYEEMIQKGD KFQWIRPISEWDPIVLNYTSGTTS
Sbjct: 181 LIDDDAAAAEESLSSSVSIRIDFVDSYEEMIQKGDSKFQWIRPISEWDPIVLNYTSGTTS 240

Query: 241 SPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFHINGWSFSWGIAAVGGTNICIR 300
           SPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTL MFHINGWSFSWGIAAVGGTN+CIR
Sbjct: 241 SPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFHINGWSFSWGIAAVGGTNVCIR 300

Query: 301 KFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGSPPPAAVLFRIE 360
           KFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGG+PPPAAVLFRIE
Sbjct: 301 KFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLDRPVKILTGGAPPPAAVLFRIE 360

Query: 361 SLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARLKARQGVRTAAVAAVDVVDSKT 420
           SLGFDVTHGYGLTETAGMVICCSWKTEWNRLPA  QARLKARQGVRTAAVAAVDVVD +T
Sbjct: 361 SLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARLKARQGVRTAAVAAVDVVDLET 420

Query: 421 GKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDEGWFLTGDVGVMHPDGYLEIKD 480
           GKSVKQDGVSIGEIVV GGSIMVGYWKDREATAKS+TDEGWFLTGDVGVMHPDGYLEIKD
Sbjct: 421 GKSVKQDGVSIGEIVVSGGSIMVGYWKDREATAKSMTDEGWFLTGDVGVMHPDGYLEIKD 480

Query: 481 RSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDEFWGETPCAFVSLREGLTRKPT 540
           RSKDVIISGGENLSSVEVES+LYTNP+VNEAAVVARPDEFWGETPCAFVSLREGLTRKPT
Sbjct: 481 RSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARPDEFWGETPCAFVSLREGLTRKPT 540

Query: 541 EEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYVLKEMAMSIGPKPSRL 594
           EEEII+YCRGKLPRFMVPKTVVF+AELPKTSTGKIKKYVLKE+AMSIG K SRL
Sbjct: 541 EEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYVLKEIAMSIGSKSSRL 594

BLAST of CmaCh04G022580 vs. NCBI nr
Match: KAG6601998.1 (putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1063.1 bits (2748), Expect = 8.5e-307
Identity = 525/555 (94.59%), Postives = 539/555 (97.12%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98
           MEDLKPR PN APLTPIGFLERAATIY + PSIIYG TTYTWSETHHRCLQLASSLSSSG
Sbjct: 1   MEDLKPRTPNCAPLTPIGFLERAATIYRDSPSIIYGHTTYTWSETHHRCLQLASSLSSSG 60

Query: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158
           IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLL HSESKLVFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLSHSESKLVFVDQA 120

Query: 159 YCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQ 218
           YCALISDALALFPPET+PPSLVLIDD AAAEESLSSS+SIR DF DSYEEMIQKGD KFQ
Sbjct: 121 YCALISDALALFPPETDPPSLVLIDDAAAAEESLSSSISIRTDFVDSYEEMIQKGDSKFQ 180

Query: 219 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFH 278
           WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTL MFH
Sbjct: 181 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLPMFH 240

Query: 279 INGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLD 338
           INGWSFSWGIAAVGGTN+CIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDN+ LD
Sbjct: 241 INGWSFSWGIAAVGGTNVCIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNQPLD 300

Query: 339 RPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARL 398
           RPVKILTGG+PPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPA  QARL
Sbjct: 301 RPVKILTGGAPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPATEQARL 360

Query: 399 KARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDE 458
           KARQGVRTAAVAAVDVVD +TGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKS+TD+
Sbjct: 361 KARQGVRTAAVAAVDVVDLETGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSMTDK 420

Query: 459 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDE 518
           GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LYTNP+VNEAAVVAR DE
Sbjct: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPSVNEAAVVARADE 480

Query: 519 FWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYV 578
           FWGETPCAFVSLREGLTRKPTEEEII+YCRGKLPRFMVPKTVVF+AELPKTSTGKIKKYV
Sbjct: 481 FWGETPCAFVSLREGLTRKPTEEEIIKYCRGKLPRFMVPKTVVFMAELPKTSTGKIKKYV 540

Query: 579 LKEMAMSIGPKPSRL 594
           LKE+AMSIGPKPSRL
Sbjct: 541 LKEIAMSIGPKPSRL 555

BLAST of CmaCh04G022580 vs. NCBI nr
Match: XP_023553079.1 (LOW QUALITY PROTEIN: probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961.1 bits (2483), Expect = 4.5e-276
Identity = 472/550 (85.82%), Postives = 504/550 (91.64%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSSG 98
           ME+LKPRP N +PLTP+GFLERAATIYG+ PSIIY  TTYTWSETHHRCLQLASSLSS+G
Sbjct: 1   MEELKPRPANFSPLTPLGFLERAATIYGDSPSIIYAHTTYTWSETHHRCLQLASSLSSAG 60

Query: 99  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQA 158
           IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTI+TRLDARTIS LL HSESKL+FVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMAGAILNTISTRLDARTISVLLCHSESKLIFVDQA 120

Query: 159 YCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKFQ 218
           Y ALI DALALFPPE N P LVLI DDA AEES SSSV I  +F  SYEEMI+KGD  F+
Sbjct: 121 YYALIQDALALFPPEINRPMLVLIADDAVAEES-SSSVLIEGNFVGSYEEMIRKGDSDFR 180

Query: 219 WIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMFH 278
           WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRG+FI   D LLEWGVPK S +LWTL MFH
Sbjct: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFIVTLDMLLEWGVPKQSTFLWTLPMFH 240

Query: 279 INGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSLD 338
           INGWS +WG+AAVGGTNICIRKFDAALI+SLIRRHHVTHMCAAPVVLNMLTNSLDNR LD
Sbjct: 241 INGWSLTWGMAAVGGTNICIRKFDAALIYSLIRRHHVTHMCAAPVVLNMLTNSLDNRPLD 300

Query: 339 RPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQARL 398
           RPVKI TGG+PPPAAVL RIE+LGFDVTHGYGLTETAG+V CCSWK EWNRLPA   ARL
Sbjct: 301 RPVKIFTGGAPPPAAVLSRIETLGFDVTHGYGLTETAGIVTCCSWKAEWNRLPATEHARL 360

Query: 399 KARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITDE 458
           KARQGVRTAA+AAVDVVD +TGKSVKQDGVSIGEIV+RGG+IM+GYWKD EATAKS+T+E
Sbjct: 361 KARQGVRTAAMAAVDVVDLETGKSVKQDGVSIGEIVLRGGTIMIGYWKDGEATAKSMTEE 420

Query: 459 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPDE 518
           GWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNP+VNEAAVVAR DE
Sbjct: 421 GWFHTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARLDE 480

Query: 519 FWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKYV 578
           FWGETPCAFVSLREGLT+KPTEEEIIEYCRGKLPRFM PKTVVF+ ELPKTSTGKI+KY+
Sbjct: 481 FWGETPCAFVSLREGLTQKPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540

Query: 579 LKEMAMSIGP 589
           L+EM MS+GP
Sbjct: 541 LREMTMSMGP 549

BLAST of CmaCh04G022580 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 738.4 bits (1905), Expect = 4.5e-213
Identity = 360/550 (65.45%), Postives = 443/550 (80.55%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYG-DTTYTWSETHHRCLQLASSLSSS 98
           ME +KP   NS PLTPIGFLERAAT+YG+  SI+YG +T YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 99  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQ 158
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 159 AYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKF 218
               L  +A+++    T+PP LV I D    EE   + V+ R  F  +Y+++I +GD  F
Sbjct: 121 FSVDLAVEAISMM--TTDPPILVFIAD--KEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180

Query: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMF 278
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG+F+ + DSL++W VPKN VYLWTL +F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240

Query: 279 HINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSL 338
           H NGWS+ WGIAAVGGTN+C+RKFDA LI+ LIR H VTHMC APVVLNML+ + + + L
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPL 300

Query: 339 DRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQAR 398
           +RPV ILT G+PPPAAVL R ES+GF ++HGYGLTETAG+ + C+WK +WNRLPA  +AR
Sbjct: 301 NRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRAR 360

Query: 399 LKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITD 458
           LKARQGVRT     +DVVD ++G+SV+++G ++GEIV+RG SIM+GY KD   T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALKN 420

Query: 459 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPD 518
            GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYTNP VNE AVVARPD
Sbjct: 421 -GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 519 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKY 578
            FWGETPCAFVSL+ GLT++PTE E+IEYCR K+P++MVPKTV FV ELPKTSTGK+ K+
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540

Query: 579 VLKEMAMSIG 588
           VL+E+A  +G
Sbjct: 541 VLREIAKKMG 545

BLAST of CmaCh04G022580 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 726.9 bits (1875), Expect = 1.3e-209
Identity = 353/552 (63.95%), Postives = 443/552 (80.25%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYG-DTTYTWSETHHRCLQLASSLSSS 98
           ME++KP   NS PLTPIGFLERAAT+YG+  SI+YG +T YTW ET+ RCL++ASSLSS 
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 99  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQ 158
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 159 AYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKF 218
               L  +A+++    T+PP LV+I D    EE   + V+    F  +Y+++I++GD  F
Sbjct: 121 FSVDLAVEAVSMM--TTDPPILVVIAD--KEEEGGVADVADLSKFSYTYDDLIERGDPGF 180

Query: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMF 278
           +WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG+F+ + DSL++W VPKN VYLWTL +F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 279 HINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRSL 338
           H NGW+  WGIAAVGGTN+C+RKFDA LI+ LIR H VTHMC APVVLNML+ + +++ L
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPL 300

Query: 339 DRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQAR 398
           + PV ILT GSPPPA VL R ES+GF ++HGYGLTETAG+++ C+WK +WN LPA  +AR
Sbjct: 301 NHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRAR 360

Query: 399 LKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSITD 458
           LKARQGVRT     +DVVD ++G SV+++G ++GEIV+RG S+M+GY KD   T K++ +
Sbjct: 361 LKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALKN 420

Query: 459 EGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPD 518
            GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYT P VNE AVVARPD
Sbjct: 421 -GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPD 480

Query: 519 EFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKKY 578
           EFWGETPCAFVSL+ G + KPTEEE++EYCR K+P++MVPKTV F+ ELPK+STGK+ K+
Sbjct: 481 EFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKF 540

Query: 579 VLKEMAMSIGPK 590
           VL+++A  +G K
Sbjct: 541 VLRDIAKKMGDK 547

BLAST of CmaCh04G022580 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 706.8 bits (1823), Expect = 1.4e-203
Identity = 353/550 (64.18%), Postives = 431/550 (78.36%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTT-YTWSETHHRCLQLASSLSSS 98
           MEDLKP   NS PLT +GFLERAAT+YG+  SI+YG++T YTW ET+HRCL +AS+LSS 
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 99  GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVDQ 158
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 159 AYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYKF 218
            Y  L  +A+ +     NPP LVLI ++   EE   + V+ R  F   Y ++I +G+  F
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANE--EEEEGGAEVTERSKFCYLYSDLITRGNPDF 180

Query: 219 QWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSMF 278
           +WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRG+F+   DSL +W VPK  VYLWTL +F
Sbjct: 181 KWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIF 240

Query: 279 HINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLD-NRS 338
           H NGW++ WGIAAVGGTN+C+RK  A  I+ LIR H VTHM  AP+VL +L+ S + ++ 
Sbjct: 241 HANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQP 300

Query: 339 LDRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQA 398
           L  PV  LT GS PPA VL R ESLGF V+HGYGLTETAG+++ C+WK  WNRLPA  QA
Sbjct: 301 LKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQA 360

Query: 399 RLKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSIT 458
           +LK+RQGVRT   + +DVVD ++G+SV++DG ++GEIV+RG SIM+GY K+   T  S  
Sbjct: 361 QLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSFK 420

Query: 459 DEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARP 518
           + GWF TGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE++LYTNP VNEAAVVARP
Sbjct: 421 N-GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARP 480

Query: 519 DEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKK 578
           DEFWGETPCAFVSL+ GLTRKPT++EIIEYC+ K+PR+M PKTV F+ ELPKTSTGKI K
Sbjct: 481 DEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIK 540

Query: 579 YVLKEMAMSI 587
            +LKE+A ++
Sbjct: 541 SLLKEIAKNM 544

BLAST of CmaCh04G022580 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 682.6 bits (1760), Expect = 2.9e-196
Identity = 331/556 (59.53%), Postives = 427/556 (76.80%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIY-GDTTYTWSETHHRCLQLASSLSSS 98
           ME L P P NS PLT +GFL+RAA++YG+ PSI++  +T +TWSETH+RCL++AS+L+SS
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 99  --GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFV 158
             GI RG VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S LL HSESKLVFV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 159 DQAYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDY 218
           D    +++ +A++ F  +   P LVL+DDD   +E  S S S   DF D+Y+ ++++GD 
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD---QEDGSLSPSAASDFLDTYQGVMERGDS 180

Query: 219 KFQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLS 278
           +F+WIRP +EW P++LNYTSGTTSSPKGVV  HR +F+    SLL+W  P   VYLWTL 
Sbjct: 181 RFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLP 240

Query: 279 MFHINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNR 338
           MFH NGW ++WG AAVG TN+C R+ DA  I+ LI +HHVTHMCAAP+VLNMLTN    +
Sbjct: 241 MFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRK 300

Query: 339 SLDRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQ 398
            L  PV+++T G+PPPAA++ R E+LGF+V HGYGLTET G V+ C+WK EW+ L  + +
Sbjct: 301 PLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLER 360

Query: 399 ARLKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSI 458
           ARLK+RQGVRT   A VDV D +TGKSV+ DGVS+GEIV++GGS+M+GY+KD E TA  +
Sbjct: 361 ARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACM 420

Query: 459 TDEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVAR 518
            ++GWF +GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE++LYTNP V EAAVVA+
Sbjct: 421 REDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAK 480

Query: 519 PDEFWGETPCAFVSLR-----EGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTS 578
           PD+ WGETPCAFVSL+      GL    TE EI E+C+ +LP++MVP+ V+F  ELPKTS
Sbjct: 481 PDKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKTS 540

Query: 579 TGKIKKYVLKEMAMSI 587
           TGKI+K++L++MA S+
Sbjct: 541 TGKIQKFLLRQMAKSL 549

BLAST of CmaCh04G022580 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 680.2 bits (1754), Expect = 1.4e-195
Identity = 325/550 (59.09%), Postives = 423/550 (76.91%), Query Frame = 0

Query: 39  MEDLKPRPPNSAPLTPIGFLERAATIYGNFPSIIYGDTTYTWSETHHRCLQLASSLSSS- 98
           ME L P   NS PLT +GFLERAA+++G+ PS+++  T +TWSETH RCL++AS+LSS+ 
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 99  -GIQRGHVVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISTLLFHSESKLVFVD 158
            GI RG VVSVI PN P +YEL FAVPM+GA+LN IN RLDA  +S LL HSESKLVFVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 159 QAYCALISDALALFPPETNPPSLVLIDDDAAAEESLSSSVSIRIDFFDSYEEMIQKGDYK 218
               +L+ +A++ F P+   P LV+++D       + SS S  +DF D+YE  +++GD +
Sbjct: 121 HHSSSLVLEAVS-FLPKDERPRLVILNDG----NDMPSSSSADMDFLDTYEGFMERGDLR 180

Query: 219 FQWIRPISEWDPIVLNYTSGTTSSPKGVVHCHRGVFIAAFDSLLEWGVPKNSVYLWTLSM 278
           F+W+RP SEW P+VLNYTSGTTSSPKGVVH HR VF++  +SLL+W +P   VYLWTL M
Sbjct: 181 FKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPM 240

Query: 279 FHINGWSFSWGIAAVGGTNICIRKFDAALIFSLIRRHHVTHMCAAPVVLNMLTNSLDNRS 338
           FH NGWS++W  AAVG  NIC+ + D   IF+LI ++ VTHMCAAP+VLNMLTN    + 
Sbjct: 241 FHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKP 300

Query: 339 LDRPVKILTGGSPPPAAVLFRIESLGFDVTHGYGLTETAGMVICCSWKTEWNRLPAIVQA 398
           L  PVK++T G+PPPA V+ + E+LGFDV+HGYG+TET G+V+ C+ K EW+RL    +A
Sbjct: 301 LQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERA 360

Query: 399 RLKARQGVRTAAVAAVDVVDSKTGKSVKQDGVSIGEIVVRGGSIMVGYWKDREATAKSIT 458
           + K+RQG+RTA  A VDV D  +GKSVK DG ++GEIV RGGS+M+GY+KD E TA S+ 
Sbjct: 361 KQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMR 420

Query: 459 DEGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARP 518
           ++GWF TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E++LYTNP + EAAVVA+P
Sbjct: 421 EDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKP 480

Query: 519 DEFWGETPCAFVSLREGLTRKPTEEEIIEYCRGKLPRFMVPKTVVFVAELPKTSTGKIKK 578
           D+ WGETPCAFVSL+       TE EI E+C+ KLP++MVP+ VVF+ ELPKTSTGKI+K
Sbjct: 481 DKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQK 540

Query: 579 YVLKEMAMSI 587
           ++L++MA S+
Sbjct: 541 FLLRQMAKSL 544