CmaCh04G022060 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G022060
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioncation/H(+) antiporter 4-like
LocationCma_Chr04: 15398326 .. 15401271 (-)
RNA-Seq ExpressionCmaCh04G022060
SyntenyCmaCh04G022060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTAAGTGAGGTAGCCGAAACAAATTTAGTTTAAAAACAGATTAAAATTAATTAAAACGAAAAAAATGATCAAAGCAAAATACCTAAATATGCTTAAAAGAACACTTTACTGTTAGAAAACAAGAACACTGTTCGTTAATCTCCACCTCATATTCCTTCTTCTTCTTCTTCTTCTTCATCTTCATCTTCATCTTCCTTATAAGCCTGTAAAAATCAGGTTTCCATAGCCATAGCTTCAAGCTTCGAAGAAACAGCCCAATTCGAAAAAATGGCTTCGAATTTCACTACTTACCAGGAGATTATGACCGCCAATAACGGCAATTTCATCACATTGTGCATGAGTTTCCCTCCAAAGGCTAGTTCCAATGGCATTTGGGATTATGTTTCTGGTTCTTCCGATACTCTTAGGTCTTCTCCTCTGCCATTGTTGGAGTGTCAGATGCTCATCATTTTTATTATCGTTACGATCCTTCATTTCTTCCTCCACTTCTTCGGCATCCCTGTTTTTGTCTCTCAAATGATTGTAAGTCTTCTTCGATGAACTTGTTTAATGTACAATTTGCCCTGGATTTTAGGGTTTTGTTTATATTAGTGGATGATTTTCAAATTTTTTTCCAAGAGTTAGATTCAAAAAACTGATGAATCTTGGCTTAGAATCTGATGATGGCTCATAGTTTCTTGTTTTCTGCTTCTTTTGTTGGTGCATTATTAACAGGCTGGTTTGATACTTGGATCATCATGGAAAGGATACTCAAATTCATTTGACAATTTCAAAGAGTATCTATTCCCCATTGTTTCTCAAGATATATTGGGATTGCTATCTGGATTTGGCTACACACTTTTTATATTTCTTGTTGGAGTTAGAATGGATTTAAATGTTGTTAAGAAGTCAGGAAAACAACCCTTGATAGGTGGTGTTTTATTAGTATTAATCTCTGCAATAATTGGCTCAATCACAGCATTTAGTTTATCAAGAGTTGACAACAGAGGTGAACTCATTAACATGGAGTTCATAGCAGCAGCCCAATCATTCACTTCATTTGCTGTTGTTCATTACCTGCTTGACTATCTCAAAATTCTGAATTCTGAAGTGGGTAGGTTAGCTCTTTCTACTGCAATTGTAGCTGATCTAACTAGTTTGAGTATCTCGTTCATCGCTACCTTCATCAGAAGCGTTCAAATTCATGGCGTTTTGAACGCTTCTATGTTTTTCACTTCGACAATCGGGTCGATTGTTTTCGTCCTGTTCATCTTTCGACCTGCAATGCTTCGGATTGCTAGATCCACACCAAATGGTAGGCCTGTGAATGATATATACATAGGCATCATTGTTCTTTTGGTATTTGTATCTATTTCAACTAATATCACTACAGGACGATCTGCTTATTCTGCTCCATTTATCTTGGGTTTGGTTGTGCCTGAAGGGCCACCATTAGGAACCAGCTTAGTGAATAGACTCGATGGCATTATTACATCGGTCTTTGTCCCACTCTTTGTCACTATTAATGTGATGAAGGCTGATCTTTCATTCCTTAGTTATAGTACAAAGTTCTTGGCTCGTTCTACGATCGTGATAATAATGACTACCGTTGCGAAAATGATCGCCTCTGTTGGTACTTCTCTGTACTTCAATATGTCTTCTTATGATGCCTTGGCTTTTGGCTTCATAATGAGTAGCAAAGGCATCATAGAGCTTGTGGGCAGCTCTTTCTTTTATGACAGCAAGGTAAGGCATGGCTTTAAATGATATAGCATTTGATATCTCTGATTTAGGAACAAAAATGAAGTAAATTTTGATCTGTTTGGTGTAAAAATTTGCAGGCCTTGACTGATCAGACTTATTCAGTGATGGTTATTGATATCTTGTTTTTCTCAACACTGATGCCTATGCTTGTGAAATGTGTATATAATCCTTCAAGGAAGTATACTCATTATAAGAGAAAAAACATTCTCAATTTGAAGCTTGACGCTGAGTTGAGAATATTAGGATGTTTTCATACACAAGAGGATGCTTCTGTTGTGCTTAATCTTCTTCATGCTTTGCACCCGACCGAGGAGTCTCCTGTTTTGTTGTATGCGCTGCACCTTGTCGAGTTGGTCGGTCGATCCTCCCCAGTTTTCATTACACATGAGCTTCATGAACAAAAGGGTTCATCTGAAGAGATGATATCAGATAATATACTTCAAATGCTTCGAAAGTATGGAAGGAGTAACGTTGTTTCGATCGAAGCATTCACAGCAATTGCCCCGAAGAGACTAATGCACGACAACATATGTACCGTAGCAATTAACAAACTTACTTCCCTTGTAATCCTTCCATTTCACCGAAGATGGACACGAGAAGGAATTGTTGAATCAGAGGATAACGCTATAAGGGCGTTAAATTGTCATGTTCTTGAGTTAGCACCGTGTTCGGTGGGAATCCTCATCGACCGTGGCTATCTTTCGAGTTACCATTCATTTGAACATTCAAATACTTCATTATTACAGGTTGCAATGGTTTTCATTGGTGGTCAAGACGATAGGGAAGCGTTTTCTTTGGCTAGACGTATGATTAAAGAGATTAACACAGCTCAACTCACAGTGATACGCCTACTTGCTGAAGATGACAACGTTAGCCATTGGGAAACGGTTCTTGACACCGAGCTGCTTAACGATGTACGGTATAGCTTTGTCGGGAGAAAAGCGGTTAGGTACGTGGAAATGCAAGCTGATGAAGGGTCAAATACAGCAGCAATAATAAGAAGCATTGGTGATTCATATGATCTTGTAATAGTTGGAAGAAGAGGTGGGGTTGAATCTCCACAGACATCAGGTTTAATGGAATGGAGTGAGTTTCCTGAGCTTGGAATTATTGGTGACATGCTTGCCTCTGCTGATTTCCATTGTAAAGCCTCCACTTTGGTGATTCAGCAACAACAACAATGCTCTTTTTACGGGCAATGA

mRNA sequence

AGTAAGTGAGGTAGCCGAAACAAATTTAGTTTAAAAACAGATTAAAATTAATTAAAACGAAAAAAATGATCAAAGCAAAATACCTAAATATGCTTAAAAGAACACTTTACTGTTAGAAAACAAGAACACTGTTCGTTAATCTCCACCTCATATTCCTTCTTCTTCTTCTTCTTCTTCATCTTCATCTTCATCTTCCTTATAAGCCTGTAAAAATCAGGTTTCCATAGCCATAGCTTCAAGCTTCGAAGAAACAGCCCAATTCGAAAAAATGGCTTCGAATTTCACTACTTACCAGGAGATTATGACCGCCAATAACGGCAATTTCATCACATTGTGCATGAGTTTCCCTCCAAAGGCTAGTTCCAATGGCATTTGGGATTATGTTTCTGGTTCTTCCGATACTCTTAGGTCTTCTCCTCTGCCATTGTTGGAGTGTCAGATGCTCATCATTTTTATTATCGTTACGATCCTTCATTTCTTCCTCCACTTCTTCGGCATCCCTGTTTTTGTCTCTCAAATGATTGCTGGTTTGATACTTGGATCATCATGGAAAGGATACTCAAATTCATTTGACAATTTCAAAGAGTATCTATTCCCCATTGTTTCTCAAGATATATTGGGATTGCTATCTGGATTTGGCTACACACTTTTTATATTTCTTGTTGGAGTTAGAATGGATTTAAATGTTGTTAAGAAGTCAGGAAAACAACCCTTGATAGGTGGTGTTTTATTAGTATTAATCTCTGCAATAATTGGCTCAATCACAGCATTTAGTTTATCAAGAGTTGACAACAGAGGTGAACTCATTAACATGGAGTTCATAGCAGCAGCCCAATCATTCACTTCATTTGCTGTTGTTCATTACCTGCTTGACTATCTCAAAATTCTGAATTCTGAAGTGGGTAGGTTAGCTCTTTCTACTGCAATTGTAGCTGATCTAACTAGTTTGAGTATCTCGTTCATCGCTACCTTCATCAGAAGCGTTCAAATTCATGGCGTTTTGAACGCTTCTATGTTTTTCACTTCGACAATCGGGTCGATTGTTTTCGTCCTGTTCATCTTTCGACCTGCAATGCTTCGGATTGCTAGATCCACACCAAATGGTAGGCCTGTGAATGATATATACATAGGCATCATTGTTCTTTTGGTATTTGTATCTATTTCAACTAATATCACTACAGGACGATCTGCTTATTCTGCTCCATTTATCTTGGGTTTGGTTGTGCCTGAAGGGCCACCATTAGGAACCAGCTTAGTGAATAGACTCGATGGCATTATTACATCGGTCTTTGTCCCACTCTTTGTCACTATTAATGTGATGAAGGCTGATCTTTCATTCCTTAGTTATAGTACAAAGTTCTTGGCTCGTTCTACGATCGTGATAATAATGACTACCGTTGCGAAAATGATCGCCTCTGTTGGTACTTCTCTGTACTTCAATATGTCTTCTTATGATGCCTTGGCTTTTGGCTTCATAATGAGTAGCAAAGGCATCATAGAGCTTGTGGGCAGCTCTTTCTTTTATGACAGCAAGGCCTTGACTGATCAGACTTATTCAGTGATGGTTATTGATATCTTGTTTTTCTCAACACTGATGCCTATGCTTGTGAAATGTGTATATAATCCTTCAAGGAAGTATACTCATTATAAGAGAAAAAACATTCTCAATTTGAAGCTTGACGCTGAGTTGAGAATATTAGGATGTTTTCATACACAAGAGGATGCTTCTGTTGTGCTTAATCTTCTTCATGCTTTGCACCCGACCGAGGAGTCTCCTGTTTTGTTGTATGCGCTGCACCTTGTCGAGTTGGTCGGTCGATCCTCCCCAGTTTTCATTACACATGAGCTTCATGAACAAAAGGGTTCATCTGAAGAGATGATATCAGATAATATACTTCAAATGCTTCGAAAGTATGGAAGGAGTAACGTTGTTTCGATCGAAGCATTCACAGCAATTGCCCCGAAGAGACTAATGCACGACAACATATGTACCGTAGCAATTAACAAACTTACTTCCCTTGTAATCCTTCCATTTCACCGAAGATGGACACGAGAAGGAATTGTTGAATCAGAGGATAACGCTATAAGGGCGTTAAATTGTCATGTTCTTGAGTTAGCACCGTGTTCGGTGGGAATCCTCATCGACCGTGGCTATCTTTCGAGTTACCATTCATTTGAACATTCAAATACTTCATTATTACAGGTTGCAATGGTTTTCATTGGTGGTCAAGACGATAGGGAAGCGTTTTCTTTGGCTAGACGTATGATTAAAGAGATTAACACAGCTCAACTCACAGTGATACGCCTACTTGCTGAAGATGACAACGTTAGCCATTGGGAAACGGTTCTTGACACCGAGCTGCTTAACGATGTACGGTATAGCTTTGTCGGGAGAAAAGCGGTTAGGTACGTGGAAATGCAAGCTGATGAAGGGTCAAATACAGCAGCAATAATAAGAAGCATTGGTGATTCATATGATCTTGTAATAGTTGGAAGAAGAGGTGGGGTTGAATCTCCACAGACATCAGGTTTAATGGAATGGAGTGAGTTTCCTGAGCTTGGAATTATTGGTGACATGCTTGCCTCTGCTGATTTCCATTGTAAAGCCTCCACTTTGGTGATTCAGCAACAACAACAATGCTCTTTTTACGGGCAATGA

Coding sequence (CDS)

ATGGCTTCGAATTTCACTACTTACCAGGAGATTATGACCGCCAATAACGGCAATTTCATCACATTGTGCATGAGTTTCCCTCCAAAGGCTAGTTCCAATGGCATTTGGGATTATGTTTCTGGTTCTTCCGATACTCTTAGGTCTTCTCCTCTGCCATTGTTGGAGTGTCAGATGCTCATCATTTTTATTATCGTTACGATCCTTCATTTCTTCCTCCACTTCTTCGGCATCCCTGTTTTTGTCTCTCAAATGATTGCTGGTTTGATACTTGGATCATCATGGAAAGGATACTCAAATTCATTTGACAATTTCAAAGAGTATCTATTCCCCATTGTTTCTCAAGATATATTGGGATTGCTATCTGGATTTGGCTACACACTTTTTATATTTCTTGTTGGAGTTAGAATGGATTTAAATGTTGTTAAGAAGTCAGGAAAACAACCCTTGATAGGTGGTGTTTTATTAGTATTAATCTCTGCAATAATTGGCTCAATCACAGCATTTAGTTTATCAAGAGTTGACAACAGAGGTGAACTCATTAACATGGAGTTCATAGCAGCAGCCCAATCATTCACTTCATTTGCTGTTGTTCATTACCTGCTTGACTATCTCAAAATTCTGAATTCTGAAGTGGGTAGGTTAGCTCTTTCTACTGCAATTGTAGCTGATCTAACTAGTTTGAGTATCTCGTTCATCGCTACCTTCATCAGAAGCGTTCAAATTCATGGCGTTTTGAACGCTTCTATGTTTTTCACTTCGACAATCGGGTCGATTGTTTTCGTCCTGTTCATCTTTCGACCTGCAATGCTTCGGATTGCTAGATCCACACCAAATGGTAGGCCTGTGAATGATATATACATAGGCATCATTGTTCTTTTGGTATTTGTATCTATTTCAACTAATATCACTACAGGACGATCTGCTTATTCTGCTCCATTTATCTTGGGTTTGGTTGTGCCTGAAGGGCCACCATTAGGAACCAGCTTAGTGAATAGACTCGATGGCATTATTACATCGGTCTTTGTCCCACTCTTTGTCACTATTAATGTGATGAAGGCTGATCTTTCATTCCTTAGTTATAGTACAAAGTTCTTGGCTCGTTCTACGATCGTGATAATAATGACTACCGTTGCGAAAATGATCGCCTCTGTTGGTACTTCTCTGTACTTCAATATGTCTTCTTATGATGCCTTGGCTTTTGGCTTCATAATGAGTAGCAAAGGCATCATAGAGCTTGTGGGCAGCTCTTTCTTTTATGACAGCAAGGCCTTGACTGATCAGACTTATTCAGTGATGGTTATTGATATCTTGTTTTTCTCAACACTGATGCCTATGCTTGTGAAATGTGTATATAATCCTTCAAGGAAGTATACTCATTATAAGAGAAAAAACATTCTCAATTTGAAGCTTGACGCTGAGTTGAGAATATTAGGATGTTTTCATACACAAGAGGATGCTTCTGTTGTGCTTAATCTTCTTCATGCTTTGCACCCGACCGAGGAGTCTCCTGTTTTGTTGTATGCGCTGCACCTTGTCGAGTTGGTCGGTCGATCCTCCCCAGTTTTCATTACACATGAGCTTCATGAACAAAAGGGTTCATCTGAAGAGATGATATCAGATAATATACTTCAAATGCTTCGAAAGTATGGAAGGAGTAACGTTGTTTCGATCGAAGCATTCACAGCAATTGCCCCGAAGAGACTAATGCACGACAACATATGTACCGTAGCAATTAACAAACTTACTTCCCTTGTAATCCTTCCATTTCACCGAAGATGGACACGAGAAGGAATTGTTGAATCAGAGGATAACGCTATAAGGGCGTTAAATTGTCATGTTCTTGAGTTAGCACCGTGTTCGGTGGGAATCCTCATCGACCGTGGCTATCTTTCGAGTTACCATTCATTTGAACATTCAAATACTTCATTATTACAGGTTGCAATGGTTTTCATTGGTGGTCAAGACGATAGGGAAGCGTTTTCTTTGGCTAGACGTATGATTAAAGAGATTAACACAGCTCAACTCACAGTGATACGCCTACTTGCTGAAGATGACAACGTTAGCCATTGGGAAACGGTTCTTGACACCGAGCTGCTTAACGATGTACGGTATAGCTTTGTCGGGAGAAAAGCGGTTAGGTACGTGGAAATGCAAGCTGATGAAGGGTCAAATACAGCAGCAATAATAAGAAGCATTGGTGATTCATATGATCTTGTAATAGTTGGAAGAAGAGGTGGGGTTGAATCTCCACAGACATCAGGTTTAATGGAATGGAGTGAGTTTCCTGAGCTTGGAATTATTGGTGACATGCTTGCCTCTGCTGATTTCCATTGTAAAGCCTCCACTTTGGTGATTCAGCAACAACAACAATGCTCTTTTTACGGGCAATGA

Protein sequence

MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLIIFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLLSGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELINMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSISTNITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSYSTKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYDSKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCFHTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISDNILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVESEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAFSLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQADEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKASTLVIQQQQQCSFYGQ
Homology
BLAST of CmaCh04G022060 vs. ExPASy Swiss-Prot
Match: Q9FYC1 (Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 2.9e-127
Identity = 286/805 (35.53%), Postives = 460/805 (57.14%), Query Frame = 0

Query: 22  LCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLIIFIIVTIL----HFFLHFFGI 81
           +C   P   SS+G+W                 +   + IIF+IVTIL    HFFL   G+
Sbjct: 21  ICGILPINPSSSGLWPSPKLPDPQANIEFWNYMFPHVQIIFLIVTILWQFFHFFLRRLGM 80

Query: 82  PVFVSQMIAGLILGSSWKGYSN------SFDNFKEYLFPIVSQDILGLLSGFGYTLFIFL 141
             F S M+ G++L  S+   +       S +++KE LF        GL+    Y +F FL
Sbjct: 81  IRFTSHMLTGILLSKSFLKENTPARKFLSTEDYKETLF--------GLVGACSYMMFWFL 140

Query: 142 VGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSR--VDNRGE----LINMEFI 201
           +GV+MDL++++ +G++ +  G+  VL+S  + ++  F + R     +GE       + FI
Sbjct: 141 MGVKMDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEPVMSFFEIIFI 200

Query: 202 AAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVL 261
              Q  +SF V+  LL  L++ NSE+GRLA+S+A+++D ++  +S +  F++ ++     
Sbjct: 201 YLIQCLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVFLKELKDDKSR 260

Query: 262 NASMFFTSTI---------GSIV----FVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVL 321
             S+F    I         G++V    F ++IFRP M  I + TP+GRPV   YI  I++
Sbjct: 261 LGSVFIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPVKKFYIYAIII 320

Query: 322 LVFVSISTNITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMK 381
           LVF S        +S +  PFILGL VP GPPLG++++ + + ++   F+P FV  +  +
Sbjct: 321 LVFGSAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFESVVFGTFLPFFVATSAEE 380

Query: 382 ADLSFLSYSTKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIEL 441
            D S L  S   L    I++ ++ + K   +   +  + M + D +A   IMS KGI E 
Sbjct: 381 IDTSILQ-SWIDLKSIVILVSVSFIVKFALTTLPAFLYGMPAKDCIALSLIMSFKGIFEF 440

Query: 442 VGSSFFYDSKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDA 501
               + Y    +   T++V+ + IL  S ++P L+K +Y+PSR Y  Y+++N+L++K ++
Sbjct: 441 GAYGYAYQRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYDPSRMYAGYEKRNMLHMKPNS 500

Query: 502 ELRILGCFHTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKG 561
           ELRIL C +  +D   ++NLL A  P+ E+PV  Y LHL+ELVG+++PV I+H L  +K 
Sbjct: 501 ELRILSCIYKTDDIRPMINLLEATCPSRENPVATYVLHLMELVGQANPVLISHRLQTRKS 560

Query: 562 SSEEMISDNILQMLRKYGRS--NVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFH 621
            +    S+N++    ++       V +  +TA++  ++MH +IC +A+N  TSL+ILPFH
Sbjct: 561 ENMSYNSENVVVSFEQFHNDFFGSVFVSTYTALSVPKMMHGDICMLALNNTTSLIILPFH 620

Query: 622 RRWTREG-IVESEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFE-HSNTSLLQVAM 681
           + W+ +G  + S+   IR LN  VL+L+PCSVGI + R         E  +N S  QV M
Sbjct: 621 QTWSADGSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRSSNGRRTIKETAANFSSYQVCM 680

Query: 682 VFIGGQDDREAFSLARRMIKEINTAQLTVIRLLAED---DNVSHWETVLDTELLNDVRYS 741
           +F+GG+DDREA SLA+RM ++ +   +TV+ L++ +   +  + W+ +LD ELL DV+ +
Sbjct: 681 LFLGGKDDREALSLAKRMARD-SRITITVVSLISSEQRANQATDWDRMLDLELLRDVKSN 740

Query: 742 FVGRKAVRYVEMQADEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELG 791
            +    + + E   ++ + T+ +++SI + YDL IVGR  G +S  T GL EWSEF ELG
Sbjct: 741 VLAGADIVFSEEVVNDANQTSQLLKSIANEYDLFIVGREKGRKSVFTEGLEEWSEFEELG 800

BLAST of CmaCh04G022060 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 6.6e-124
Identity = 279/811 (34.40%), Postives = 463/811 (57.09%), Query Frame = 0

Query: 22  LCMSFPPKASSNGIWDYVSGSSDTLR----SSPLPLLECQMLIIFIIVTILHFFLHFFGI 81
           +C   P   SSNG+W     S   +     +   P L+   LII  +   LHFFL   G+
Sbjct: 22  ICDVLPINPSSNGVWPQQKFSDPNINVHFWNYAFPHLQMIFLIISFLWQFLHFFLRRLGM 81

Query: 82  PVFVSQMIAGLILGSSWKGYSN------SFDNFKEYLFPIVSQDILGLLSGFGYTLFIFL 141
             F S M+ G++L  S+   ++      S +++KE +F         L +   Y +F FL
Sbjct: 82  IRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVF--------SLTAACSYMMFWFL 141

Query: 142 VGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSR---VDNRGELIN-MEFIA- 201
           +GV+MD  +++ +G++ +  G+  VL+S ++ S+  F   R     N    +N +E++  
Sbjct: 142 MGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVI 201

Query: 202 -AAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVL 261
            + Q  +SF VV  LL  L++ NSE+GRLA+S+A+++D ++  ++ +  F++ ++     
Sbjct: 202 YSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQTR 261

Query: 262 NASMFFTSTIGS----------IVFV---LFIFRPAMLRIARSTPNGRPVNDIYIGIIVL 321
             S+F    I            ++FV   +++FRP M  I + TP+GRPV  IY+  I++
Sbjct: 262 LGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIV 321

Query: 322 LVFVSISTNITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMK 381
           +V  S        +S +  PFILGL VP GPPLG++++ + +  I   F+P F+  +  +
Sbjct: 322 MVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFFIASSSTE 381

Query: 382 ADLSFLSYSTKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIEL 441
            D+S L +  + L    ++++ + V K I +   +L++ M   D  A   IMS KGI EL
Sbjct: 382 IDISAL-FGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMSFKGIFEL 441

Query: 442 VGSSFFYDSKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDA 501
              +  Y   ++  +T++V  + I   S ++P +++ +Y+PSR Y  Y+++N+ +LK ++
Sbjct: 442 GAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNMQHLKPNS 501

Query: 502 ELRILGCFHTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKG 561
           ELRIL C +  +D S ++NLL A+ P+ ESPV  Y LHL+ELVG+++P+FI+H+L  ++ 
Sbjct: 502 ELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISHKLQTRR- 561

Query: 562 SSEEMISDNILQMLRKYGRS--NVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFH 621
           + E   S+N+L    K+ +     V +  +TA++    MH +IC +A+N  TSL++LPFH
Sbjct: 562 TEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSLILLPFH 621

Query: 622 RRWTREG-IVESEDNAIRALNCHVLELAPCSVGILI-----DRGYLSSYHSFEHS---NT 681
           + W+ +G  + S +N IR LN  VL++APCSVG+ +      R  +SS     +    N 
Sbjct: 622 QTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTINGTVPNL 681

Query: 682 SLLQVAMVFIGGQDDREAFSLARRMIKEINTAQLTVIRLLAEDDNVSH---WETVLDTEL 741
           S   + M+F+GG+DDREA +LA RM ++     +T++RL+  D+       W+ +LD EL
Sbjct: 682 SSYNICMIFLGGKDDREAVTLATRMARD-PRINITIVRLITTDEKARENTVWDKMLDDEL 741

Query: 742 LNDVRYSFVGRKAVRYVEMQADEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEW 790
           L DV+ + +    + Y E   ++ + T++++RS+   +D+ IVGR  G  S  T GL EW
Sbjct: 742 LRDVKSNTL--VDIFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFTEGLEEW 801

BLAST of CmaCh04G022060 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 9.6e-115
Identity = 263/788 (33.38%), Postives = 435/788 (55.20%), Query Frame = 0

Query: 27  PPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLIIFIIVTILHFFLHFFGIPVFVSQMIA 86
           P   ++NG+W       D      LPL   Q+ ++ ++     F L  F  P  +S+++ 
Sbjct: 18  PSMITTNGVW-----QGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEILG 77

Query: 87  GLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLLSGFGYTLFIFLVGVRMDLNVVKKSGK 146
           G++LG S  G S     F   +FP  S  +L  ++  G   F+FLVGV MD+ VV+K+GK
Sbjct: 78  GIVLGPSVLGRST---KFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGK 137

Query: 147 QPL---IGGVLLVLISAIIGSITAFSLSR-VDNRGELINMEFIAAAQSFTSFAVVHYLLD 206
           + L   IGG++L     +IG+  +FS+ R  D+ G+   + F+  A S T+F V+  +L 
Sbjct: 138 RALTIAIGGMVLPF---LIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILA 197

Query: 207 YLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVLNASMFFTSTIGSIVFVL 266
            LK++N+E+GR+++S A+V D+ +  +  +A  +         +  +  +S +  I   +
Sbjct: 198 ELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAV-FIAVCV 257

Query: 267 FIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSISTNITTGRSAYSAPFILGLVVPEG 326
           F+ RP +  I R TP G   ++ +I +I+  V +S       G  +    F+ GLV+P G
Sbjct: 258 FVVRPGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG 317

Query: 327 PPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSYSTKFLARSTIVIIMTTVAKMIA 386
            PLG +L+ +L+  ++ + +PLF  I+ +K +++ +     +L    +VI +    K+I 
Sbjct: 318 -PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLT-LFLVIFLACAGKVIG 377

Query: 387 SVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYDSKALTDQTYSVMVIDILFFSTL 446
           +V  + +  M   + +  G ++++KG++E++  +   D K L D+T++ MV+  L  + +
Sbjct: 378 TVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGV 437

Query: 447 MPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCFHTQEDASVVLNLLHALHPTEES 506
           +  +V  +Y P +K   YKR+ I   K D+ELR+L C HT  +   ++NLL A HPT+ S
Sbjct: 438 ITPIVTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRS 497

Query: 507 PVLLYALHLVELVGRSSPVFITHELHEQKG---SSEEMISDNILQMLRKYGR-SNVVSIE 566
           P+ +Y LHLVEL GR+S + I H   +      +  +  SD+I+     Y + +  V+++
Sbjct: 498 PICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQHAAFVAVQ 557

Query: 567 AFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVESEDNAIRALNCHVLELAP 626
             TAI+P   MH+++C++A +K  S +I+PFH++ T +G +ES + A R +N ++LE +P
Sbjct: 558 PLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSP 617

Query: 627 CSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAFSLARRMIKEINTAQLTVI 686
           CSVGIL+DRG   +     +SNT  LQVA++F GG DDREA + A RM +      LTV+
Sbjct: 618 CSVGILVDRGLNGATRL--NSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGIT-LTVL 677

Query: 687 RLLAEDDNVSHWET-------------------VLDTELLNDVRYSFVGRKAVRYVEMQA 746
           R + ++D      T                    LD + +N  R      +++ Y+E   
Sbjct: 678 RFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLV 737

Query: 747 DEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCK 788
             G  T A +RS+  S+DL IVGR  G+ SP T+GL +WSE PELG IGD+LAS+DF   
Sbjct: 738 SNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAAT 788

BLAST of CmaCh04G022060 vs. ExPASy Swiss-Prot
Match: Q9FYB9 (Cation/H(+) antiporter 11 OS=Arabidopsis thaliana OX=3702 GN=CHX11 PE=2 SV=2)

HSP 1 Score: 409.5 bits (1051), Expect = 9.0e-113
Identity = 261/788 (33.12%), Postives = 426/788 (54.06%), Query Frame = 0

Query: 15  NNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLIIFIIVTILHFFLHF 74
           N   +I  C       SS G W+ +  S D +    LPLLE Q+++IF  + + H FL  
Sbjct: 2   NTTTYIGHCRISFFNISSQGFWENLK-SPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRC 61

Query: 75  FGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLLSGFGYTLFIFLVGV 134
            G+   VS MIAGLILG                   +     L  +S FG  +F FL+ V
Sbjct: 62  IGVSQIVSYMIAGLILGPQLFDILEKSSGKLSADPALDGTAALRCISVFGRLMFTFLMTV 121

Query: 135 RMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNR-----GELINMEFIAAAQ 194
           R    V   SGK P++ G++         S        +D         L     I   Q
Sbjct: 122 RTSRRVAFHSGKLPVVIGIVSFFAPLFSLSFLNLFTDNIDPHYMSLDKALAERTVIVITQ 181

Query: 195 SFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVLNASM 254
           S        Y+L  LKI+NSE+GRLALS + + D+  +    +AT  ++  IH  ++ ++
Sbjct: 182 SQILLPSTTYILLELKIINSELGRLALSASAINDMLGIFAMIVAT-TQATYIH--VSHAI 241

Query: 255 FFTSTIGSIVF---VLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSISTNITTGR 314
            +   +  I+F   V F+F+P +  I   TP  +PV DIYI  ++L  F S +  +    
Sbjct: 242 AYRDLVAVIIFFLIVFFVFKPMVQWIIDRTPEDKPVEDIYIHAVILTAFASAAYFVFFNM 301

Query: 315 SAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKAD-LSFLSYSTKFL 374
                P I+G+++PEGPPLG++L  + + +  +VF+P+ +T + M+ D L  LS  T  +
Sbjct: 302 KYVLGPLIIGIIIPEGPPLGSALEAKFERLTMNVFLPISITFSAMRCDGLRILSQFTD-I 361

Query: 375 ARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYDSKALT 434
             +  + ++  V K++A +   LY+ +   ++LA   I+S K  +E V      + K ++
Sbjct: 362 YFNIFLTLLILVIKLVACLTLCLYYKLPRSESLAVSLILSYKSFVEFVLYEAVLEEKFIS 421

Query: 435 DQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCFHTQED 494
             TY+ +++  L  + ++PM+V+ +Y+P RKY +Y++++IL+L+ ++ LRIL C H  E+
Sbjct: 422 QATYAFLILYSLLSAGIVPMVVRSMYDPKRKYVNYQKRDILHLEANSGLRILTCLHKPEN 481

Query: 495 ASVVLNLLHAL-HPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISDNILQ 554
            S  +  L     P  + P+ +  LHLV+LVG+ +P+ ++H+   ++      I    L 
Sbjct: 482 VSETIAFLQLFSSPIHDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRLHKNSYIHTANL- 541

Query: 555 MLRKYGRSNV--VSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVESE 614
             R++ + ++  V++  FTA + + LMH++ICT+A+++ TS++++P  R+WT +G+ ES+
Sbjct: 542 AFRQFMQESLESVTVTTFTAFSHENLMHEDICTLALDRTTSMIVVPSGRKWTVDGMFESD 601

Query: 615 DNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAFSL 674
           D A R LN  +L+ APCS+GIL+DRG  S        N   + V ++FIGG+DDREA SL
Sbjct: 602 DLAARQLNQSLLDRAPCSIGILVDRGQFSRKSYVTSKNRYNIDVGVLFIGGKDDREALSL 661

Query: 675 ARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQADEG 734
            +RM K     ++TVIRL+ + +  S W+ +LD E L D++ S    + + Y E      
Sbjct: 662 VKRM-KYNPRVRVTVIRLIFDHEIESEWDYILDNEGLKDLK-STESNEDILYTERIVTSV 721

Query: 735 SNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKAST 791
                 ++ + + YDL++VGR   + S   SGL EW E PELG+IGD+LA+ D + K S 
Sbjct: 722 VEVVKAVQLLAEEYDLMVVGRDHDMTSQDLSGLTEWVELPELGVIGDLLAARDLNSKVSV 781

BLAST of CmaCh04G022060 vs. ExPASy Swiss-Prot
Match: Q9FYC0 (Cation/H(+) antiporter 12 OS=Arabidopsis thaliana OX=3702 GN=CHX12 PE=2 SV=2)

HSP 1 Score: 409.5 bits (1051), Expect = 9.0e-113
Identity = 261/784 (33.29%), Postives = 424/784 (54.08%), Query Frame = 0

Query: 15  NNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLIIFIIVTILHFFLHF 74
           N  ++I  C+      SS G W+ +  S D +    LPL+E Q+L+IF+ + I+H FL  
Sbjct: 2   NTTSYIRGCIPLVFNISSFGFWENLK-SPDVIFGYSLPLMEFQILLIFVFIIIIHSFLKS 61

Query: 75  FGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLLSGFGYTLFIFLVGV 134
           FGI    S M+AGLILG               +   +     L  LS  G  +  F + V
Sbjct: 62  FGISPIPSYMLAGLILGPQLFNLREVSSRKLSWDPALDGNGPLRGLSVCGNIMLAFFMTV 121

Query: 135 RMDLNVVKKSGKQPLIGGVLLVLISAIIG-SITAFSLSRVD------NRGELINMEFIAA 194
           ++   +   +G  P++ G L  ++  + G  +       +D      N+     +  I++
Sbjct: 122 KISRRLAFNNGWLPIVIGTLSFIVPFLGGFCVRNLHTDNIDPYYMSPNKVLAERIVVISS 181

Query: 195 AQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVLNA 254
             S     VVH+L + LKILNSE+GRL LS +++ D+ + ++S  A  + + +    + A
Sbjct: 182 QSSILLPTVVHFLSE-LKILNSELGRLVLSASLINDIFASTVSIFAYLVGTYKNISPMTA 241

Query: 255 SMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSISTNITTGRS 314
                + I  I+    + RP +  I   TP G+PV D+Y+  +VL V  S + +      
Sbjct: 242 YRDLIAVIILILVAFCVLRPVVEWIVERTPEGKPVADVYVHAVVLSVIASAAYSSFFNMK 301

Query: 315 AYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSYSTKFLAR 374
               PF+LG+++PEGPP+G++L  + + +  +V +P+ +T + M+ D+  + Y    +  
Sbjct: 302 YLLGPFLLGIIIPEGPPIGSALEAKYEALTMNVLIPISITFSTMRCDVMKIVYQYDDIWY 361

Query: 375 STIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYDSKALTDQ 434
           +  ++  T   KM   +   LY  +   +A+A   ++ SK   E+      YD   ++  
Sbjct: 362 NIFLMTFTGFLKMATGMVPCLYCKIPFKEAIAASLLLCSKSFSEIFLYESTYDDSYISQA 421

Query: 435 TYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCFHTQEDAS 494
           TY+ ++   L  S ++P  +  +Y+P RKY  Y++KNI+NLK D++LRIL C H  E+ S
Sbjct: 422 TYTFLITCALINSGIIPTALAGLYDPKRKYVGYQKKNIMNLKPDSDLRILTCIHRPENIS 481

Query: 495 VVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISDNILQMLR 554
             ++ L  L     S +++  LHLV+LVG++ PV I+H     K  +  + +  I     
Sbjct: 482 AAISFLQFL----PSTIVVTVLHLVKLVGKTVPVLISH----NKQINRVVTNSYIHTANL 541

Query: 555 KYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVESEDNAIR 614
            + +   V++  FTAI  + LMHD IC VA+ + TS++I+P  R+WT +G  ESED AIR
Sbjct: 542 AFSQLESVTMTMFTAITHENLMHDEICKVALEQATSIIIVPSGRKWTVDGAFESEDEAIR 601

Query: 615 ALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAFSLARRMI 674
            LN  +L+ A CS+GIL+DRG L    S + +    + V ++FIGG+DDREA SL ++M 
Sbjct: 602 RLNESLLKSASCSIGILVDRGQL----SLKGTRKFNIDVGVIFIGGKDDREALSLVKKM- 661

Query: 675 KEINTAQLTVIRLLAEDDNVS-HWETVLDTELLNDVRYSFVGRKAVRYVEMQADEGSNTA 734
           K+    ++TVIRL+++ +  S +W+ +LD E+L D++       ++ Y E     G   A
Sbjct: 662 KQNPRVKITVIRLISDRETESTNWDYILDHEVLEDLK-DTEATNSIAYTERIVTGGPEVA 721

Query: 735 AIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKASTLVIQ 791
             +RS+ + YDL++VGR  G+ SP   GLMEW E PELG+IGD+LAS +   + S LV+Q
Sbjct: 722 TTVRSLSEDYDLMVVGRDHGMASPDFDGLMEWVELPELGVIGDLLASRELDSRVSVLVVQ 769

BLAST of CmaCh04G022060 vs. ExPASy TrEMBL
Match: A0A6J1JNK5 (cation/H(+) antiporter 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487482 PE=4 SV=1)

HSP 1 Score: 1524.6 bits (3946), Expect = 0.0e+00
Identity = 796/796 (100.00%), Postives = 796/796 (100.00%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI
Sbjct: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL
Sbjct: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI
Sbjct: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ
Sbjct: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
           IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST
Sbjct: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY
Sbjct: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
           STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD
Sbjct: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF
Sbjct: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD
Sbjct: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540

Query: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE 600
           NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE
Sbjct: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE 600

Query: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660
           SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF
Sbjct: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660

Query: 661 SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD 720
           SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD
Sbjct: 661 SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD 720

Query: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA 780
           EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA
Sbjct: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA 780

Query: 781 STLVIQQQQQCSFYGQ 797
           STLVIQQQQQCSFYGQ
Sbjct: 781 STLVIQQQQQCSFYGQ 796

BLAST of CmaCh04G022060 vs. ExPASy TrEMBL
Match: A0A6J1GPY8 (cation/H(+) antiporter 4-like OS=Cucurbita moschata OX=3662 GN=LOC111456471 PE=4 SV=1)

HSP 1 Score: 1465.3 bits (3792), Expect = 0.0e+00
Identity = 763/796 (95.85%), Postives = 780/796 (97.99%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           MASNFT YQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI
Sbjct: 1   MASNFTIYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IFIIVTILHFFLH FGIPVFVSQMIAGLILGSSWKGYS SFDNFKEYLFPI SQDILGLL
Sbjct: 61  IFIIVTILHFFLHVFGIPVFVSQMIAGLILGSSWKGYSISFDNFKEYLFPIASQDILGLL 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           SGFGYTLF+FLVGVRMDLNVVKKSGKQPLIGGVL V+ISAIIGSITAFSLSRVD RGELI
Sbjct: 121 SGFGYTLFVFLVGVRMDLNVVKKSGKQPLIGGVLSVVISAIIGSITAFSLSRVDKRGELI 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           NME+IAAAQSFTSFAVVHYLLD+LKILNSEVGRLALST IVADLTSLSISFIATFIRSVQ
Sbjct: 181 NMEYIAAAQSFTSFAVVHYLLDHLKILNSEVGRLALSTTIVADLTSLSISFIATFIRSVQ 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
           IHGVL ASM FTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST
Sbjct: 241 IHGVLKASMSFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           ++T GRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFL+Y
Sbjct: 301 HVTIGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLTY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
           STKFLARSTIVIIMTTVAKMIASVGTSLYF MSSYDALAFGFIMSSKGIIELVGSSFFYD
Sbjct: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFKMSSYDALAFGFIMSSKGIIELVGSSFFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           SKALT QTYSVMVIDILFFSTL+PMLVKCVYNPSRKY HYKRKNILNLKLDAELRILGCF
Sbjct: 421 SKALTGQTYSVMVIDILFFSTLVPMLVKCVYNPSRKYAHYKRKNILNLKLDAELRILGCF 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQED SVVLNLLHAL+PTEESPVLLY LHLVELVGRSSPVFI+HELHEQKG+SEEMISD
Sbjct: 481 HTQEDVSVVLNLLHALYPTEESPVLLYTLHLVELVGRSSPVFISHELHEQKGASEEMISD 540

Query: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE 600
           NILQMLRKYGRSNVVSIEAFTAIAPKRLMHD+ICTVAINKLTSLVILPFHRRWTREGIVE
Sbjct: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDDICTVAINKLTSLVILPFHRRWTREGIVE 600

Query: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660
           SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF
Sbjct: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660

Query: 661 SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD 720
           SLARRMIKE+NTAQLTVIRLLAEDDN+S+WETVLDTELLNDVRYSFVG KAVRY+EMQAD
Sbjct: 661 SLARRMIKEMNTAQLTVIRLLAEDDNISYWETVLDTELLNDVRYSFVGGKAVRYMEMQAD 720

Query: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA 780
           EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEW+EFPELGIIGDMLASADFHCKA
Sbjct: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWNEFPELGIIGDMLASADFHCKA 780

Query: 781 STLVIQQQQQCSFYGQ 797
           STLVIQQQQQCSFYGQ
Sbjct: 781 STLVIQQQQQCSFYGQ 796

BLAST of CmaCh04G022060 vs. ExPASy TrEMBL
Match: A0A1S3AUL2 (cation/H(+) antiporter 4-like OS=Cucumis melo OX=3656 GN=LOC103483015 PE=4 SV=1)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 560/798 (70.18%), Postives = 661/798 (82.83%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           MASNFT Y  I  + +GNF+TLC   PPK +S+GIWD+V G +  +RSSPLPLLE QML+
Sbjct: 1   MASNFTIYNNIFGSVHGNFLTLCFHTPPKINSDGIWDFVFGYASKIRSSPLPLLELQMLL 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IF ++ ILH FLH FG+PVFVSQMIAGLILGSSW+G  +SFDNFK+ +F   SQ+I+ LL
Sbjct: 61  IFFVIIILHCFLHLFGLPVFVSQMIAGLILGSSWRGSFSSFDNFKDDVFMTASQEIVSLL 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           +GFGYTLF+FL+GVRMDL+VVK+SG+Q LIGGVL ++I AI+GS+TAF  SR+    E  
Sbjct: 121 AGFGYTLFVFLIGVRMDLSVVKRSGRQSLIGGVLSIVIPAILGSLTAFGFSRLGKTHETA 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           NMEF+AA QS+TSFAVV  LLD+LKILNSEVGRL LST IVADL  LS SFI T + +VQ
Sbjct: 181 NMEFVAANQSYTSFAVVVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSFIITVVENVQ 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
             G L+  M     +GS+V V+F+FRPAML I RSTP+GRPV D YI II++LV VS +T
Sbjct: 241 SQGALSGLMTIALAVGSMVLVVFLFRPAMLWIVRSTPSGRPVPDGYICIIIVLVLVSSAT 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           +   GR+ YS PFILGL+VPEGPPLG SLVN+LD IITSVFVPLFVTI VMK DLSFL Y
Sbjct: 301 SNIMGRTVYSGPFILGLIVPEGPPLGASLVNKLDSIITSVFVPLFVTICVMKVDLSFLLY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
             +F   STIVI ++T+ K+  SVGT+LYF MSS+DALAFG IM +KGI+EL   SFFYD
Sbjct: 361 DGEFFIYSTIVIFISTIGKLAVSVGTALYFKMSSHDALAFGLIMCTKGIVELAACSFFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           S  L+ QT++V+++DIL FS LMPMLVK  Y+PSRKY+HY++KNILNLK DAEL ILGC 
Sbjct: 421 SNLLSKQTFAVLIVDILIFSILMPMLVKWFYDPSRKYSHYQKKNILNLKPDAELSILGCI 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQ+D  V+LNLL A  PTEESPV LYALHLVELVGR++PVFITHELH++K SSE M+SD
Sbjct: 481 HTQDDLPVLLNLLDASCPTEESPVSLYALHLVELVGRATPVFITHELHDKKCSSEVMVSD 540

Query: 541 NILQMLRKYGRSN--VVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGI 600
           +++QMLRKY  SN  VVSIEAFTAIAP +LMHD+ICTVA+NKLTS++ILPFHRRWTREG 
Sbjct: 541 SLIQMLRKYEMSNEGVVSIEAFTAIAPMKLMHDDICTVAVNKLTSIIILPFHRRWTREGF 600

Query: 601 VESEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDRE 660
           V+SEDN IRALNC VLE APCSVGILIDRG+L SY SF  S  SLLQVAMVFIGGQDDRE
Sbjct: 601 VDSEDNTIRALNCQVLERAPCSVGILIDRGHLLSYRSFGGSCASLLQVAMVFIGGQDDRE 660

Query: 661 AFSLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQ 720
           AFS ARRM+KE++TAQLTVIRLLAED+++SHWE VLDTELLNDV++SFVG +  RYVE +
Sbjct: 661 AFSFARRMVKELSTAQLTVIRLLAEDESISHWEMVLDTELLNDVKHSFVGGEPFRYVEKR 720

Query: 721 ADEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHC 780
           ADEGS TA+I+RS+GD YDL+IVGRR G++SPQTSGLMEW+EFPELGIIGDMLASAD H 
Sbjct: 721 ADEGSETASIVRSLGDEYDLIIVGRREGIDSPQTSGLMEWNEFPELGIIGDMLASADSHV 780

Query: 781 KASTLVIQQQQQCSFYGQ 797
           KASTLV+QQQQQ SFY Q
Sbjct: 781 KASTLVVQQQQQWSFYKQ 798

BLAST of CmaCh04G022060 vs. ExPASy TrEMBL
Match: A0A5D3BL54 (Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold446G00720 PE=4 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 560/798 (70.18%), Postives = 660/798 (82.71%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           MASNFT Y  I  + +GNF+TLC   PPK +S+GIWD+V G +  +RSSPLPLLE QML+
Sbjct: 1   MASNFTIYNNIFGSVHGNFLTLCFHTPPKINSDGIWDFVFGYAFKIRSSPLPLLELQMLL 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IF ++ ILH FLH FG+PVFVSQMIAGLILGSSW+G  +SFDNFK+ +F   SQ+I+ LL
Sbjct: 61  IFFVIIILHCFLHLFGLPVFVSQMIAGLILGSSWRGSFSSFDNFKDDVFMTASQEIVSLL 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           +GFGYTLF+FL+GVRMDL+VVK+SG+Q LIGGVL ++I AI+GS+TAF  SR+    E  
Sbjct: 121 AGFGYTLFVFLIGVRMDLSVVKRSGRQSLIGGVLSIVIPAILGSLTAFGFSRLGKTHETA 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           NMEF+AA QS+TSFAVV  LLD+LKILNSEVGRL LST IVADL  LS SFI T + +VQ
Sbjct: 181 NMEFVAANQSYTSFAVVVCLLDHLKILNSEVGRLVLSTTIVADLVGLSFSFIVTVVENVQ 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
             G L+  M     +GS+V V+F+FRPAML I RSTP+GRPV D YI II+LLV VS +T
Sbjct: 241 SQGALSGLMTIALAVGSMVLVVFLFRPAMLWIVRSTPSGRPVPDGYICIIILLVLVSSAT 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           +   GR+ YS PFILGL+VPEGPPLG SLVN+LD IITSVFVPLFVTI VMK DLSFL Y
Sbjct: 301 SNIMGRTVYSGPFILGLIVPEGPPLGASLVNKLDSIITSVFVPLFVTICVMKVDLSFLLY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
             +F   STIVI ++T+ K+  SVGT+LYF MSS+DALAFG IM +KGI+EL   SFFYD
Sbjct: 361 DGEFFIYSTIVIFISTIGKLAVSVGTALYFKMSSHDALAFGLIMCTKGIVELAACSFFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           S  L+ QT++V+++DIL FS LMPMLVK  Y+PSRKY+HY++KNILNLK DAEL ILGC 
Sbjct: 421 SNLLSKQTFAVLIVDILIFSILMPMLVKWFYDPSRKYSHYQKKNILNLKPDAELSILGCI 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQ+D  V+LNLL A  PTEESPV LYALHLVELVGR++PVFITHEL ++K SSE M+SD
Sbjct: 481 HTQDDLPVLLNLLDASCPTEESPVSLYALHLVELVGRATPVFITHELQDKKCSSEVMVSD 540

Query: 541 NILQMLRKYGRSN--VVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGI 600
           +++QMLRKY  SN  VVSIEAFTAIAP +LMHD+ICTVA+NKLTS++ILPFHRRWTREG 
Sbjct: 541 SLIQMLRKYEMSNEGVVSIEAFTAIAPMKLMHDDICTVAVNKLTSIIILPFHRRWTREGF 600

Query: 601 VESEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDRE 660
           V+SEDN IRALNC VLE APCSVGILIDRG+L SY SF  S  SLLQVAMVFIGGQDDRE
Sbjct: 601 VDSEDNTIRALNCQVLERAPCSVGILIDRGHLLSYRSFGGSCASLLQVAMVFIGGQDDRE 660

Query: 661 AFSLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQ 720
           AFS ARRM+KE++TAQLTVIRLLAED+++SHWE VLDTELLNDV++SFVG +  RYVE +
Sbjct: 661 AFSFARRMVKELSTAQLTVIRLLAEDESISHWEMVLDTELLNDVKHSFVGGEPFRYVEKR 720

Query: 721 ADEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHC 780
           ADEGS TA+I+RS+GD YDL+IVGRR G++SPQTSGLMEW+EFPELGIIGDMLASAD H 
Sbjct: 721 ADEGSETASIVRSLGDEYDLIIVGRREGIDSPQTSGLMEWNEFPELGIIGDMLASADSHV 780

Query: 781 KASTLVIQQQQQCSFYGQ 797
           KASTLV+QQQQQ SFY Q
Sbjct: 781 KASTLVVQQQQQWSFYKQ 798

BLAST of CmaCh04G022060 vs. ExPASy TrEMBL
Match: A0A6J1DUA7 (cation/H(+) antiporter 4-like OS=Momordica charantia OX=3673 GN=LOC111024501 PE=4 SV=1)

HSP 1 Score: 1035.0 bits (2675), Expect = 1.6e-298
Identity = 529/792 (66.79%), Postives = 645/792 (81.44%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           M +N+T Y EIMT  +GNFIT C++ PPK +S+GIWD V G S+  R +PLPLLE QML 
Sbjct: 1   MDTNYTIYDEIMTTESGNFITFCLTLPPKINSHGIWDSVLGHSNVERPTPLPLLELQMLT 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IF +  +LHFFL   G+PVFVSQMIAGLILGSSW+G S SFD FK++LF I SQ+ILG++
Sbjct: 61  IFCVTMLLHFFLQLLGLPVFVSQMIAGLILGSSWRGNSESFDKFKDFLFSIGSQEILGMV 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           +GFGYTLF+FL+GVRMDL VVK+SG+Q LI GVL +++ A++G + A  LSR     E  
Sbjct: 121 TGFGYTLFVFLIGVRMDLGVVKRSGRQTLITGVLSIIVPALLGLMAAVGLSRFGEEDEAA 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           N+EFIAA QS+TSFAVV  LL++LKILNSEVGRL LS++IVAD+  LS SFI + + +V 
Sbjct: 181 NLEFIAANQSYTSFAVVVSLLEHLKILNSEVGRLVLSSSIVADMVGLSFSFIVSVVENVH 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
            HGV  AS+ F  TI S+V VLF+FRP ML I RSTP+GRPV D YI +I+LLV VS  T
Sbjct: 241 SHGVFGASIGFIFTILSVVIVLFVFRPVMLWIVRSTPHGRPVQDGYICLIILLVLVSSVT 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           +   GR+ YS PF+LGLVVPEGPPLG SLVN+LDGIITSVF+PLF+TI V+KADLSF++Y
Sbjct: 301 SNIMGRTIYSGPFLLGLVVPEGPPLGASLVNKLDGIITSVFIPLFITIAVIKADLSFINY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
           S  FLA+S  VI++T + KM   VGTSLYF MSSYDALAFG IMSSKGI+EL  SS+FYD
Sbjct: 361 SGLFLAKSMTVILITIMGKMAVCVGTSLYFKMSSYDALAFGLIMSSKGIVELAASSYFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           SK L+ QT++V+V+DIL  S LMPMLVK +Y+PSRKY  Y+++NILNLK +AEL +LGC 
Sbjct: 421 SKVLSHQTFAVLVVDILIISILMPMLVKWIYDPSRKYIGYQKRNILNLKPNAELSVLGCL 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQ D  V+LNL+ A  PTE+SP+ LYALH+ ELVGR++PVFI+HEL +QKGS +E++S 
Sbjct: 481 HTQNDVPVLLNLIDASCPTEDSPISLYALHVEELVGRATPVFISHELQDQKGSYKELLSG 540

Query: 541 NILQMLRKYGRSN--VVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGI 600
           NI+QMLRKY R+N  VVSIE FTAIAP +LMH++ICT+A  KLTSL+ILPFHR+WT+EG 
Sbjct: 541 NIIQMLRKYERNNRDVVSIEVFTAIAPMKLMHEDICTLAARKLTSLIILPFHRKWTKEGY 600

Query: 601 VESEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDRE 660
           +ESEDN IRALNCHVL+ APCSVGILIDRG L+S   F HS T  LQVAM+FIGG DDRE
Sbjct: 601 IESEDNNIRALNCHVLDRAPCSVGILIDRGNLTSSLPFGHSRTPSLQVAMIFIGGPDDRE 660

Query: 661 AFSLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQ 720
           AFS A RM+K+++ AQLTVIRLLAED++VSHWE VLDTELLND+++SFVG +   Y+E +
Sbjct: 661 AFSFAXRMVKDLSIAQLTVIRLLAEDESVSHWEKVLDTELLNDMKHSFVGGERFAYLERR 720

Query: 721 ADEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHC 780
           ADEGS TAAI+RS+ D YDL+IVGRR GVESPQTSGLMEW+EFPELGI+GDMLASAD  C
Sbjct: 721 ADEGSETAAIVRSVCDEYDLIIVGRRDGVESPQTSGLMEWNEFPELGIVGDMLASADSDC 780

Query: 781 KASTLVIQQQQQ 791
           +ASTLV+QQQQQ
Sbjct: 781 RASTLVVQQQQQ 792

BLAST of CmaCh04G022060 vs. NCBI nr
Match: XP_022990661.1 (cation/H(+) antiporter 4-like [Cucurbita maxima])

HSP 1 Score: 1524.6 bits (3946), Expect = 0.0e+00
Identity = 796/796 (100.00%), Postives = 796/796 (100.00%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI
Sbjct: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL
Sbjct: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI
Sbjct: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ
Sbjct: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
           IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST
Sbjct: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY
Sbjct: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
           STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD
Sbjct: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF
Sbjct: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD
Sbjct: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540

Query: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE 600
           NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE
Sbjct: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE 600

Query: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660
           SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF
Sbjct: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660

Query: 661 SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD 720
           SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD
Sbjct: 661 SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD 720

Query: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA 780
           EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA
Sbjct: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA 780

Query: 781 STLVIQQQQQCSFYGQ 797
           STLVIQQQQQCSFYGQ
Sbjct: 781 STLVIQQQQQCSFYGQ 796

BLAST of CmaCh04G022060 vs. NCBI nr
Match: XP_022954106.1 (cation/H(+) antiporter 4-like [Cucurbita moschata])

HSP 1 Score: 1465.3 bits (3792), Expect = 0.0e+00
Identity = 763/796 (95.85%), Postives = 780/796 (97.99%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           MASNFT YQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI
Sbjct: 1   MASNFTIYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IFIIVTILHFFLH FGIPVFVSQMIAGLILGSSWKGYS SFDNFKEYLFPI SQDILGLL
Sbjct: 61  IFIIVTILHFFLHVFGIPVFVSQMIAGLILGSSWKGYSISFDNFKEYLFPIASQDILGLL 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           SGFGYTLF+FLVGVRMDLNVVKKSGKQPLIGGVL V+ISAIIGSITAFSLSRVD RGELI
Sbjct: 121 SGFGYTLFVFLVGVRMDLNVVKKSGKQPLIGGVLSVVISAIIGSITAFSLSRVDKRGELI 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           NME+IAAAQSFTSFAVVHYLLD+LKILNSEVGRLALST IVADLTSLSISFIATFIRSVQ
Sbjct: 181 NMEYIAAAQSFTSFAVVHYLLDHLKILNSEVGRLALSTTIVADLTSLSISFIATFIRSVQ 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
           IHGVL ASM FTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST
Sbjct: 241 IHGVLKASMSFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           ++T GRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFL+Y
Sbjct: 301 HVTIGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLTY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
           STKFLARSTIVIIMTTVAKMIASVGTSLYF MSSYDALAFGFIMSSKGIIELVGSSFFYD
Sbjct: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFKMSSYDALAFGFIMSSKGIIELVGSSFFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           SKALT QTYSVMVIDILFFSTL+PMLVKCVYNPSRKY HYKRKNILNLKLDAELRILGCF
Sbjct: 421 SKALTGQTYSVMVIDILFFSTLVPMLVKCVYNPSRKYAHYKRKNILNLKLDAELRILGCF 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQED SVVLNLLHAL+PTEESPVLLY LHLVELVGRSSPVFI+HELHEQKG+SEEMISD
Sbjct: 481 HTQEDVSVVLNLLHALYPTEESPVLLYTLHLVELVGRSSPVFISHELHEQKGASEEMISD 540

Query: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE 600
           NILQMLRKYGRSNVVSIEAFTAIAPKRLMHD+ICTVAINKLTSLVILPFHRRWTREGIVE
Sbjct: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDDICTVAINKLTSLVILPFHRRWTREGIVE 600

Query: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660
           SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF
Sbjct: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660

Query: 661 SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD 720
           SLARRMIKE+NTAQLTVIRLLAEDDN+S+WETVLDTELLNDVRYSFVG KAVRY+EMQAD
Sbjct: 661 SLARRMIKEMNTAQLTVIRLLAEDDNISYWETVLDTELLNDVRYSFVGGKAVRYMEMQAD 720

Query: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA 780
           EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEW+EFPELGIIGDMLASADFHCKA
Sbjct: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWNEFPELGIIGDMLASADFHCKA 780

Query: 781 STLVIQQQQQCSFYGQ 797
           STLVIQQQQQCSFYGQ
Sbjct: 781 STLVIQQQQQCSFYGQ 796

BLAST of CmaCh04G022060 vs. NCBI nr
Match: KAG6601948.1 (Cation/H(+) antiporter 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1461.8 bits (3783), Expect = 0.0e+00
Identity = 762/796 (95.73%), Postives = 777/796 (97.61%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           MASNFT YQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLE QMLI
Sbjct: 1   MASNFTIYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLEFQMLI 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IFIIVTILHFFLH FGIPVFVSQMIAGLILGSSWKGYS SFDNFKEYLFPI SQDILGLL
Sbjct: 61  IFIIVTILHFFLHVFGIPVFVSQMIAGLILGSSWKGYSISFDNFKEYLFPIASQDILGLL 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           SGFGYTLF+FLVGVRMDLNVVKKSGKQPLIGGVL V+ISAIIGS TAFSLSRVD RGELI
Sbjct: 121 SGFGYTLFVFLVGVRMDLNVVKKSGKQPLIGGVLSVVISAIIGSFTAFSLSRVDKRGELI 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           NME+IAA QSFTSFAVVHYLLD+LKILNSEVGRLALST IVADLTSLSISFIATFIRSVQ
Sbjct: 181 NMEYIAAGQSFTSFAVVHYLLDHLKILNSEVGRLALSTTIVADLTSLSISFIATFIRSVQ 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
           IHGVL ASM FTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGI+VLLVFVSIST
Sbjct: 241 IHGVLKASMSFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIVVLLVFVSIST 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           +IT GRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFL+Y
Sbjct: 301 HITIGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLTY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
           STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD
Sbjct: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           SKALT QTYSVMVIDILFFSTL+PMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF
Sbjct: 421 SKALTGQTYSVMVIDILFFSTLVPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQED SVVLNLLHAL+PTEESPVLLY LHLVELVGRSSPVFI+HELHEQKGSSEEMISD
Sbjct: 481 HTQEDVSVVLNLLHALYPTEESPVLLYTLHLVELVGRSSPVFISHELHEQKGSSEEMISD 540

Query: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE 600
           NILQMLRKYGRSNVVSIEAFTAIAPKRLMHD+ICTVAINKLTSLVILPFHRRWTREGIVE
Sbjct: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDDICTVAINKLTSLVILPFHRRWTREGIVE 600

Query: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660
           SEDNAIR LNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF
Sbjct: 601 SEDNAIRVLNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660

Query: 661 SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD 720
           S ARRMIKE+NTAQLTVIRLLAEDD++SHWETVLDTELLNDVRYSFVG KAVRYVEMQAD
Sbjct: 661 SFARRMIKEMNTAQLTVIRLLAEDDSISHWETVLDTELLNDVRYSFVGGKAVRYVEMQAD 720

Query: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA 780
           EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEW+EFPELGIIGDMLASADFHCKA
Sbjct: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWNEFPELGIIGDMLASADFHCKA 780

Query: 781 STLVIQQQQQCSFYGQ 797
           STLVIQQQQQCSFYGQ
Sbjct: 781 STLVIQQQQQCSFYGQ 796

BLAST of CmaCh04G022060 vs. NCBI nr
Match: KAG7032643.1 (Cation/H(+) antiporter 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1461.0 bits (3781), Expect = 0.0e+00
Identity = 761/796 (95.60%), Postives = 776/796 (97.49%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           MASNFT YQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLE QMLI
Sbjct: 1   MASNFTIYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLEFQMLI 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IFIIVTILHFFLH FGIPVFVSQMIAGLILGSSWKGYS SFDNFKEYLFPI SQDILGLL
Sbjct: 61  IFIIVTILHFFLHVFGIPVFVSQMIAGLILGSSWKGYSISFDNFKEYLFPIASQDILGLL 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           SGFGYTLF+FLVGVRMDLNVVKKSGKQPLIGGVL V+ISAIIGS TAFSLSRVD RGELI
Sbjct: 121 SGFGYTLFVFLVGVRMDLNVVKKSGKQPLIGGVLSVVISAIIGSFTAFSLSRVDKRGELI 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           NME+IAA QSFTSFAVVHYLLD+LKILNSEVGRLALST IVADLTSLSISFIATFIRSVQ
Sbjct: 181 NMEYIAAGQSFTSFAVVHYLLDHLKILNSEVGRLALSTTIVADLTSLSISFIATFIRSVQ 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
           IHGVL ASM FTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST
Sbjct: 241 IHGVLKASMSFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           +IT GRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFL+Y
Sbjct: 301 HITIGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLTY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
           STKFLARSTIVIIMTT+AKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD
Sbjct: 361 STKFLARSTIVIIMTTIAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           SKALT QTYSVMVIDILFFSTL+PMLVKCVYNPSRKYTHYKRKN+LNLKLDAELRILGCF
Sbjct: 421 SKALTGQTYSVMVIDILFFSTLVPMLVKCVYNPSRKYTHYKRKNVLNLKLDAELRILGCF 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQED SVVLNLLHAL+PTEESPVLLY LHLVELVGRSSPVFI+HELHEQKGSSEEMISD
Sbjct: 481 HTQEDVSVVLNLLHALYPTEESPVLLYTLHLVELVGRSSPVFISHELHEQKGSSEEMISD 540

Query: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE 600
           NILQMLRKYGRSNVVSIEAFTAIAPKRLMHD+ICTVAINKLTSLVILPFHRRWTREGIVE
Sbjct: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDDICTVAINKLTSLVILPFHRRWTREGIVE 600

Query: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660
           SEDNAIR LNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF
Sbjct: 601 SEDNAIRVLNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660

Query: 661 SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD 720
           S ARRMIKE+NTAQLTVIRLLAEDDN+SHWE VLDTELLNDVRYSFVG KAVRYVEMQAD
Sbjct: 661 SFARRMIKEMNTAQLTVIRLLAEDDNISHWEMVLDTELLNDVRYSFVGGKAVRYVEMQAD 720

Query: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA 780
           EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEW+EFPELGIIGDMLASADFHCKA
Sbjct: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWNEFPELGIIGDMLASADFHCKA 780

Query: 781 STLVIQQQQQCSFYGQ 797
           STLVIQQQQQCSFYGQ
Sbjct: 781 STLVIQQQQQCSFYGQ 796

BLAST of CmaCh04G022060 vs. NCBI nr
Match: XP_023550239.1 (cation/H(+) antiporter 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1458.4 bits (3774), Expect = 0.0e+00
Identity = 760/796 (95.48%), Postives = 776/796 (97.49%), Query Frame = 0

Query: 1   MASNFTTYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60
           MASNFT YQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI
Sbjct: 1   MASNFTIYQEIMTANNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLI 60

Query: 61  IFIIVTILHFFLHFFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLL 120
           IFIIVTILHF LH FGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPI SQDILGLL
Sbjct: 61  IFIIVTILHFLLHVFGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIASQDILGLL 120

Query: 121 SGFGYTLFIFLVGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNRGELI 180
           SGFGYTLFIFLVGVRMDLN VKKSGKQPLIGGVL V+ISAIIGSI AFSL+RVDNRGELI
Sbjct: 121 SGFGYTLFIFLVGVRMDLNAVKKSGKQPLIGGVLSVVISAIIGSIAAFSLARVDNRGELI 180

Query: 181 NMEFIAAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQ 240
           NMEFIAAAQSFTSFAVVHYLLD+LKILNSEVGR+ALST IVADLTSLSISFIATFIRSVQ
Sbjct: 181 NMEFIAAAQSFTSFAVVHYLLDHLKILNSEVGRIALSTTIVADLTSLSISFIATFIRSVQ 240

Query: 241 IHGVLNASMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSIST 300
           IHGVL ASM FTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVND+YIGIIVLLVFVS+ST
Sbjct: 241 IHGVLKASMSFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDVYIGIIVLLVFVSVST 300

Query: 301 NITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360
           +IT GRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY
Sbjct: 301 HITIGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSY 360

Query: 361 STKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420
           ST FLARS IVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD
Sbjct: 361 STTFLARSMIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYD 420

Query: 421 SKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480
           SKALT QTYSVMVIDILFFSTL+PMLVK VYNPSRKYTHYKRKNILNLKLDAELRILGCF
Sbjct: 421 SKALTGQTYSVMVIDILFFSTLVPMLVKYVYNPSRKYTHYKRKNILNLKLDAELRILGCF 480

Query: 481 HTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISD 540
           HTQED SV+LNLLHAL+PTEESPVLLY LHLVELVGRSSPVFI+HELHEQKGSSEEMISD
Sbjct: 481 HTQEDVSVLLNLLHALYPTEESPVLLYTLHLVELVGRSSPVFISHELHEQKGSSEEMISD 540

Query: 541 NILQMLRKYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVE 600
           NILQMLRKYGRSNVVSIEAFTAI PKRLMHD+ICTVAINKLTSLVILPFHRRWTREGIVE
Sbjct: 541 NILQMLRKYGRSNVVSIEAFTAIVPKRLMHDDICTVAINKLTSLVILPFHRRWTREGIVE 600

Query: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660
           SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF
Sbjct: 601 SEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAF 660

Query: 661 SLARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQAD 720
           SLARRMIKE+NTAQLTVIRLLAEDD++SHWETVLDTELLNDVRYSFVG KAVRYVEMQAD
Sbjct: 661 SLARRMIKEMNTAQLTVIRLLAEDDSISHWETVLDTELLNDVRYSFVGGKAVRYVEMQAD 720

Query: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKA 780
           EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEW+EFPELGIIGDMLASADFHCKA
Sbjct: 721 EGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWNEFPELGIIGDMLASADFHCKA 780

Query: 781 STLVIQQQQQCSFYGQ 797
           STLVIQQQQQCS YGQ
Sbjct: 781 STLVIQQQQQCSVYGQ 796

BLAST of CmaCh04G022060 vs. TAIR 10
Match: AT3G44900.1 (cation/H+ exchanger 4 )

HSP 1 Score: 457.6 bits (1176), Expect = 2.0e-128
Identity = 286/805 (35.53%), Postives = 460/805 (57.14%), Query Frame = 0

Query: 22  LCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLIIFIIVTIL----HFFLHFFGI 81
           +C   P   SS+G+W                 +   + IIF+IVTIL    HFFL   G+
Sbjct: 21  ICGILPINPSSSGLWPSPKLPDPQANIEFWNYMFPHVQIIFLIVTILWQFFHFFLRRLGM 80

Query: 82  PVFVSQMIAGLILGSSWKGYSN------SFDNFKEYLFPIVSQDILGLLSGFGYTLFIFL 141
             F S M+ G++L  S+   +       S +++KE LF        GL+    Y +F FL
Sbjct: 81  IRFTSHMLTGILLSKSFLKENTPARKFLSTEDYKETLF--------GLVGACSYMMFWFL 140

Query: 142 VGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSR--VDNRGE----LINMEFI 201
           +GV+MDL++++ +G++ +  G+  VL+S  + ++  F + R     +GE       + FI
Sbjct: 141 MGVKMDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEPVMSFFEIIFI 200

Query: 202 AAAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVL 261
              Q  +SF V+  LL  L++ NSE+GRLA+S+A+++D ++  +S +  F++ ++     
Sbjct: 201 YLIQCLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVFLKELKDDKSR 260

Query: 262 NASMFFTSTI---------GSIV----FVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVL 321
             S+F    I         G++V    F ++IFRP M  I + TP+GRPV   YI  I++
Sbjct: 261 LGSVFIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPVKKFYIYAIII 320

Query: 322 LVFVSISTNITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMK 381
           LVF S        +S +  PFILGL VP GPPLG++++ + + ++   F+P FV  +  +
Sbjct: 321 LVFGSAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFESVVFGTFLPFFVATSAEE 380

Query: 382 ADLSFLSYSTKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIEL 441
            D S L  S   L    I++ ++ + K   +   +  + M + D +A   IMS KGI E 
Sbjct: 381 IDTSILQ-SWIDLKSIVILVSVSFIVKFALTTLPAFLYGMPAKDCIALSLIMSFKGIFEF 440

Query: 442 VGSSFFYDSKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDA 501
               + Y    +   T++V+ + IL  S ++P L+K +Y+PSR Y  Y+++N+L++K ++
Sbjct: 441 GAYGYAYQRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYDPSRMYAGYEKRNMLHMKPNS 500

Query: 502 ELRILGCFHTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKG 561
           ELRIL C +  +D   ++NLL A  P+ E+PV  Y LHL+ELVG+++PV I+H L  +K 
Sbjct: 501 ELRILSCIYKTDDIRPMINLLEATCPSRENPVATYVLHLMELVGQANPVLISHRLQTRKS 560

Query: 562 SSEEMISDNILQMLRKYGRS--NVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFH 621
            +    S+N++    ++       V +  +TA++  ++MH +IC +A+N  TSL+ILPFH
Sbjct: 561 ENMSYNSENVVVSFEQFHNDFFGSVFVSTYTALSVPKMMHGDICMLALNNTTSLIILPFH 620

Query: 622 RRWTREG-IVESEDNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFE-HSNTSLLQVAM 681
           + W+ +G  + S+   IR LN  VL+L+PCSVGI + R         E  +N S  QV M
Sbjct: 621 QTWSADGSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRSSNGRRTIKETAANFSSYQVCM 680

Query: 682 VFIGGQDDREAFSLARRMIKEINTAQLTVIRLLAED---DNVSHWETVLDTELLNDVRYS 741
           +F+GG+DDREA SLA+RM ++ +   +TV+ L++ +   +  + W+ +LD ELL DV+ +
Sbjct: 681 LFLGGKDDREALSLAKRMARD-SRITITVVSLISSEQRANQATDWDRMLDLELLRDVKSN 740

Query: 742 FVGRKAVRYVEMQADEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELG 791
            +    + + E   ++ + T+ +++SI + YDL IVGR  G +S  T GL EWSEF ELG
Sbjct: 741 VLAGADIVFSEEVVNDANQTSQLLKSIANEYDLFIVGREKGRKSVFTEGLEEWSEFEELG 800

BLAST of CmaCh04G022060 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 446.4 bits (1147), Expect = 4.7e-125
Identity = 279/811 (34.40%), Postives = 463/811 (57.09%), Query Frame = 0

Query: 22  LCMSFPPKASSNGIWDYVSGSSDTLR----SSPLPLLECQMLIIFIIVTILHFFLHFFGI 81
           +C   P   SSNG+W     S   +     +   P L+   LII  +   LHFFL   G+
Sbjct: 22  ICDVLPINPSSNGVWPQQKFSDPNINVHFWNYAFPHLQMIFLIISFLWQFLHFFLRRLGM 81

Query: 82  PVFVSQMIAGLILGSSWKGYSN------SFDNFKEYLFPIVSQDILGLLSGFGYTLFIFL 141
             F S M+ G++L  S+   ++      S +++KE +F         L +   Y +F FL
Sbjct: 82  IRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVF--------SLTAACSYMMFWFL 141

Query: 142 VGVRMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSR---VDNRGELIN-MEFIA- 201
           +GV+MD  +++ +G++ +  G+  VL+S ++ S+  F   R     N    +N +E++  
Sbjct: 142 MGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVI 201

Query: 202 -AAQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVL 261
            + Q  +SF VV  LL  L++ NSE+GRLA+S+A+++D ++  ++ +  F++ ++     
Sbjct: 202 YSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQTR 261

Query: 262 NASMFFTSTIGS----------IVFV---LFIFRPAMLRIARSTPNGRPVNDIYIGIIVL 321
             S+F    I            ++FV   +++FRP M  I + TP+GRPV  IY+  I++
Sbjct: 262 LGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIV 321

Query: 322 LVFVSISTNITTGRSAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMK 381
           +V  S        +S +  PFILGL VP GPPLG++++ + +  I   F+P F+  +  +
Sbjct: 322 MVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFFIASSSTE 381

Query: 382 ADLSFLSYSTKFLARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIEL 441
            D+S L +  + L    ++++ + V K I +   +L++ M   D  A   IMS KGI EL
Sbjct: 382 IDISAL-FGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMSFKGIFEL 441

Query: 442 VGSSFFYDSKALTDQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDA 501
              +  Y   ++  +T++V  + I   S ++P +++ +Y+PSR Y  Y+++N+ +LK ++
Sbjct: 442 GAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNMQHLKPNS 501

Query: 502 ELRILGCFHTQEDASVVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKG 561
           ELRIL C +  +D S ++NLL A+ P+ ESPV  Y LHL+ELVG+++P+FI+H+L  ++ 
Sbjct: 502 ELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISHKLQTRR- 561

Query: 562 SSEEMISDNILQMLRKYGRS--NVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFH 621
           + E   S+N+L    K+ +     V +  +TA++    MH +IC +A+N  TSL++LPFH
Sbjct: 562 TEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSLILLPFH 621

Query: 622 RRWTREG-IVESEDNAIRALNCHVLELAPCSVGILI-----DRGYLSSYHSFEHS---NT 681
           + W+ +G  + S +N IR LN  VL++APCSVG+ +      R  +SS     +    N 
Sbjct: 622 QTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTINGTVPNL 681

Query: 682 SLLQVAMVFIGGQDDREAFSLARRMIKEINTAQLTVIRLLAEDDNVSH---WETVLDTEL 741
           S   + M+F+GG+DDREA +LA RM ++     +T++RL+  D+       W+ +LD EL
Sbjct: 682 SSYNICMIFLGGKDDREAVTLATRMARD-PRINITIVRLITTDEKARENTVWDKMLDDEL 741

Query: 742 LNDVRYSFVGRKAVRYVEMQADEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEW 790
           L DV+ + +    + Y E   ++ + T++++RS+   +D+ IVGR  G  S  T GL EW
Sbjct: 742 LRDVKSNTL--VDIFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFTEGLEEW 801

BLAST of CmaCh04G022060 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 416.0 bits (1068), Expect = 6.8e-116
Identity = 263/788 (33.38%), Postives = 435/788 (55.20%), Query Frame = 0

Query: 27  PPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLIIFIIVTILHFFLHFFGIPVFVSQMIA 86
           P   ++NG+W       D      LPL   Q+ ++ ++     F L  F  P  +S+++ 
Sbjct: 18  PSMITTNGVW-----QGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEILG 77

Query: 87  GLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLLSGFGYTLFIFLVGVRMDLNVVKKSGK 146
           G++LG S  G S     F   +FP  S  +L  ++  G   F+FLVGV MD+ VV+K+GK
Sbjct: 78  GIVLGPSVLGRST---KFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGK 137

Query: 147 QPL---IGGVLLVLISAIIGSITAFSLSR-VDNRGELINMEFIAAAQSFTSFAVVHYLLD 206
           + L   IGG++L     +IG+  +FS+ R  D+ G+   + F+  A S T+F V+  +L 
Sbjct: 138 RALTIAIGGMVLPF---LIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILA 197

Query: 207 YLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVLNASMFFTSTIGSIVFVL 266
            LK++N+E+GR+++S A+V D+ +  +  +A  +         +  +  +S +  I   +
Sbjct: 198 ELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAV-FIAVCV 257

Query: 267 FIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSISTNITTGRSAYSAPFILGLVVPEG 326
           F+ RP +  I R TP G   ++ +I +I+  V +S       G  +    F+ GLV+P G
Sbjct: 258 FVVRPGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG 317

Query: 327 PPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSYSTKFLARSTIVIIMTTVAKMIA 386
            PLG +L+ +L+  ++ + +PLF  I+ +K +++ +     +L    +VI +    K+I 
Sbjct: 318 -PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLT-LFLVIFLACAGKVIG 377

Query: 387 SVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYDSKALTDQTYSVMVIDILFFSTL 446
           +V  + +  M   + +  G ++++KG++E++  +   D K L D+T++ MV+  L  + +
Sbjct: 378 TVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGV 437

Query: 447 MPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCFHTQEDASVVLNLLHALHPTEES 506
           +  +V  +Y P +K   YKR+ I   K D+ELR+L C HT  +   ++NLL A HPT+ S
Sbjct: 438 ITPIVTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRS 497

Query: 507 PVLLYALHLVELVGRSSPVFITHELHEQKG---SSEEMISDNILQMLRKYGR-SNVVSIE 566
           P+ +Y LHLVEL GR+S + I H   +      +  +  SD+I+     Y + +  V+++
Sbjct: 498 PICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQHAAFVAVQ 557

Query: 567 AFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVESEDNAIRALNCHVLELAP 626
             TAI+P   MH+++C++A +K  S +I+PFH++ T +G +ES + A R +N ++LE +P
Sbjct: 558 PLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSP 617

Query: 627 CSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAFSLARRMIKEINTAQLTVI 686
           CSVGIL+DRG   +     +SNT  LQVA++F GG DDREA + A RM +      LTV+
Sbjct: 618 CSVGILVDRGLNGATRL--NSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGIT-LTVL 677

Query: 687 RLLAEDDNVSHWET-------------------VLDTELLNDVRYSFVGRKAVRYVEMQA 746
           R + ++D      T                    LD + +N  R      +++ Y+E   
Sbjct: 678 RFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLV 737

Query: 747 DEGSNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCK 788
             G  T A +RS+  S+DL IVGR  G+ SP T+GL +WSE PELG IGD+LAS+DF   
Sbjct: 738 SNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAAT 788

BLAST of CmaCh04G022060 vs. TAIR 10
Match: AT3G44920.1 (cation/H+ exchanger 11 )

HSP 1 Score: 409.5 bits (1051), Expect = 6.4e-114
Identity = 261/788 (33.12%), Postives = 426/788 (54.06%), Query Frame = 0

Query: 15  NNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLIIFIIVTILHFFLHF 74
           N   +I  C       SS G W+ +  S D +    LPLLE Q+++IF  + + H FL  
Sbjct: 2   NTTTYIGHCRISFFNISSQGFWENLK-SPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRC 61

Query: 75  FGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLLSGFGYTLFIFLVGV 134
            G+   VS MIAGLILG                   +     L  +S FG  +F FL+ V
Sbjct: 62  IGVSQIVSYMIAGLILGPQLFDILEKSSGKLSADPALDGTAALRCISVFGRLMFTFLMTV 121

Query: 135 RMDLNVVKKSGKQPLIGGVLLVLISAIIGSITAFSLSRVDNR-----GELINMEFIAAAQ 194
           R    V   SGK P++ G++         S        +D         L     I   Q
Sbjct: 122 RTSRRVAFHSGKLPVVIGIVSFFAPLFSLSFLNLFTDNIDPHYMSLDKALAERTVIVITQ 181

Query: 195 SFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVLNASM 254
           S        Y+L  LKI+NSE+GRLALS + + D+  +    +AT  ++  IH  ++ ++
Sbjct: 182 SQILLPSTTYILLELKIINSELGRLALSASAINDMLGIFAMIVAT-TQATYIH--VSHAI 241

Query: 255 FFTSTIGSIVF---VLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSISTNITTGR 314
            +   +  I+F   V F+F+P +  I   TP  +PV DIYI  ++L  F S +  +    
Sbjct: 242 AYRDLVAVIIFFLIVFFVFKPMVQWIIDRTPEDKPVEDIYIHAVILTAFASAAYFVFFNM 301

Query: 315 SAYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKAD-LSFLSYSTKFL 374
                P I+G+++PEGPPLG++L  + + +  +VF+P+ +T + M+ D L  LS  T  +
Sbjct: 302 KYVLGPLIIGIIIPEGPPLGSALEAKFERLTMNVFLPISITFSAMRCDGLRILSQFTD-I 361

Query: 375 ARSTIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYDSKALT 434
             +  + ++  V K++A +   LY+ +   ++LA   I+S K  +E V      + K ++
Sbjct: 362 YFNIFLTLLILVIKLVACLTLCLYYKLPRSESLAVSLILSYKSFVEFVLYEAVLEEKFIS 421

Query: 435 DQTYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCFHTQED 494
             TY+ +++  L  + ++PM+V+ +Y+P RKY +Y++++IL+L+ ++ LRIL C H  E+
Sbjct: 422 QATYAFLILYSLLSAGIVPMVVRSMYDPKRKYVNYQKRDILHLEANSGLRILTCLHKPEN 481

Query: 495 ASVVLNLLHAL-HPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISDNILQ 554
            S  +  L     P  + P+ +  LHLV+LVG+ +P+ ++H+   ++      I    L 
Sbjct: 482 VSETIAFLQLFSSPIHDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRLHKNSYIHTANL- 541

Query: 555 MLRKYGRSNV--VSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVESE 614
             R++ + ++  V++  FTA + + LMH++ICT+A+++ TS++++P  R+WT +G+ ES+
Sbjct: 542 AFRQFMQESLESVTVTTFTAFSHENLMHEDICTLALDRTTSMIVVPSGRKWTVDGMFESD 601

Query: 615 DNAIRALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAFSL 674
           D A R LN  +L+ APCS+GIL+DRG  S        N   + V ++FIGG+DDREA SL
Sbjct: 602 DLAARQLNQSLLDRAPCSIGILVDRGQFSRKSYVTSKNRYNIDVGVLFIGGKDDREALSL 661

Query: 675 ARRMIKEINTAQLTVIRLLAEDDNVSHWETVLDTELLNDVRYSFVGRKAVRYVEMQADEG 734
            +RM K     ++TVIRL+ + +  S W+ +LD E L D++ S    + + Y E      
Sbjct: 662 VKRM-KYNPRVRVTVIRLIFDHEIESEWDYILDNEGLKDLK-STESNEDILYTERIVTSV 721

Query: 735 SNTAAIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKAST 791
                 ++ + + YDL++VGR   + S   SGL EW E PELG+IGD+LA+ D + K S 
Sbjct: 722 VEVVKAVQLLAEEYDLMVVGRDHDMTSQDLSGLTEWVELPELGVIGDLLAARDLNSKVSV 781

BLAST of CmaCh04G022060 vs. TAIR 10
Match: AT3G44910.1 (cation/H+ exchanger 12 )

HSP 1 Score: 409.5 bits (1051), Expect = 6.4e-114
Identity = 261/784 (33.29%), Postives = 424/784 (54.08%), Query Frame = 0

Query: 15  NNGNFITLCMSFPPKASSNGIWDYVSGSSDTLRSSPLPLLECQMLIIFIIVTILHFFLHF 74
           N  ++I  C+      SS G W+ +  S D +    LPL+E Q+L+IF+ + I+H FL  
Sbjct: 2   NTTSYIRGCIPLVFNISSFGFWENLK-SPDVIFGYSLPLMEFQILLIFVFIIIIHSFLKS 61

Query: 75  FGIPVFVSQMIAGLILGSSWKGYSNSFDNFKEYLFPIVSQDILGLLSGFGYTLFIFLVGV 134
           FGI    S M+AGLILG               +   +     L  LS  G  +  F + V
Sbjct: 62  FGISPIPSYMLAGLILGPQLFNLREVSSRKLSWDPALDGNGPLRGLSVCGNIMLAFFMTV 121

Query: 135 RMDLNVVKKSGKQPLIGGVLLVLISAIIG-SITAFSLSRVD------NRGELINMEFIAA 194
           ++   +   +G  P++ G L  ++  + G  +       +D      N+     +  I++
Sbjct: 122 KISRRLAFNNGWLPIVIGTLSFIVPFLGGFCVRNLHTDNIDPYYMSPNKVLAERIVVISS 181

Query: 195 AQSFTSFAVVHYLLDYLKILNSEVGRLALSTAIVADLTSLSISFIATFIRSVQIHGVLNA 254
             S     VVH+L + LKILNSE+GRL LS +++ D+ + ++S  A  + + +    + A
Sbjct: 182 QSSILLPTVVHFLSE-LKILNSELGRLVLSASLINDIFASTVSIFAYLVGTYKNISPMTA 241

Query: 255 SMFFTSTIGSIVFVLFIFRPAMLRIARSTPNGRPVNDIYIGIIVLLVFVSISTNITTGRS 314
                + I  I+    + RP +  I   TP G+PV D+Y+  +VL V  S + +      
Sbjct: 242 YRDLIAVIILILVAFCVLRPVVEWIVERTPEGKPVADVYVHAVVLSVIASAAYSSFFNMK 301

Query: 315 AYSAPFILGLVVPEGPPLGTSLVNRLDGIITSVFVPLFVTINVMKADLSFLSYSTKFLAR 374
               PF+LG+++PEGPP+G++L  + + +  +V +P+ +T + M+ D+  + Y    +  
Sbjct: 302 YLLGPFLLGIIIPEGPPIGSALEAKYEALTMNVLIPISITFSTMRCDVMKIVYQYDDIWY 361

Query: 375 STIVIIMTTVAKMIASVGTSLYFNMSSYDALAFGFIMSSKGIIELVGSSFFYDSKALTDQ 434
           +  ++  T   KM   +   LY  +   +A+A   ++ SK   E+      YD   ++  
Sbjct: 362 NIFLMTFTGFLKMATGMVPCLYCKIPFKEAIAASLLLCSKSFSEIFLYESTYDDSYISQA 421

Query: 435 TYSVMVIDILFFSTLMPMLVKCVYNPSRKYTHYKRKNILNLKLDAELRILGCFHTQEDAS 494
           TY+ ++   L  S ++P  +  +Y+P RKY  Y++KNI+NLK D++LRIL C H  E+ S
Sbjct: 422 TYTFLITCALINSGIIPTALAGLYDPKRKYVGYQKKNIMNLKPDSDLRILTCIHRPENIS 481

Query: 495 VVLNLLHALHPTEESPVLLYALHLVELVGRSSPVFITHELHEQKGSSEEMISDNILQMLR 554
             ++ L  L     S +++  LHLV+LVG++ PV I+H     K  +  + +  I     
Sbjct: 482 AAISFLQFL----PSTIVVTVLHLVKLVGKTVPVLISH----NKQINRVVTNSYIHTANL 541

Query: 555 KYGRSNVVSIEAFTAIAPKRLMHDNICTVAINKLTSLVILPFHRRWTREGIVESEDNAIR 614
            + +   V++  FTAI  + LMHD IC VA+ + TS++I+P  R+WT +G  ESED AIR
Sbjct: 542 AFSQLESVTMTMFTAITHENLMHDEICKVALEQATSIIIVPSGRKWTVDGAFESEDEAIR 601

Query: 615 ALNCHVLELAPCSVGILIDRGYLSSYHSFEHSNTSLLQVAMVFIGGQDDREAFSLARRMI 674
            LN  +L+ A CS+GIL+DRG L    S + +    + V ++FIGG+DDREA SL ++M 
Sbjct: 602 RLNESLLKSASCSIGILVDRGQL----SLKGTRKFNIDVGVIFIGGKDDREALSLVKKM- 661

Query: 675 KEINTAQLTVIRLLAEDDNVS-HWETVLDTELLNDVRYSFVGRKAVRYVEMQADEGSNTA 734
           K+    ++TVIRL+++ +  S +W+ +LD E+L D++       ++ Y E     G   A
Sbjct: 662 KQNPRVKITVIRLISDRETESTNWDYILDHEVLEDLK-DTEATNSIAYTERIVTGGPEVA 721

Query: 735 AIIRSIGDSYDLVIVGRRGGVESPQTSGLMEWSEFPELGIIGDMLASADFHCKASTLVIQ 791
             +RS+ + YDL++VGR  G+ SP   GLMEW E PELG+IGD+LAS +   + S LV+Q
Sbjct: 722 TTVRSLSEDYDLMVVGRDHGMASPDFDGLMEWVELPELGVIGDLLASRELDSRVSVLVVQ 769

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FYC12.9e-12735.53Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1[more]
Q9FFB86.6e-12434.40Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Q9SIT59.6e-11533.38Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1[more]
Q9FYB99.0e-11333.12Cation/H(+) antiporter 11 OS=Arabidopsis thaliana OX=3702 GN=CHX11 PE=2 SV=2[more]
Q9FYC09.0e-11333.29Cation/H(+) antiporter 12 OS=Arabidopsis thaliana OX=3702 GN=CHX12 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1JNK50.0e+00100.00cation/H(+) antiporter 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487482 PE=4 S... [more]
A0A6J1GPY80.0e+0095.85cation/H(+) antiporter 4-like OS=Cucurbita moschata OX=3662 GN=LOC111456471 PE=4... [more]
A0A1S3AUL20.0e+0070.18cation/H(+) antiporter 4-like OS=Cucumis melo OX=3656 GN=LOC103483015 PE=4 SV=1[more]
A0A5D3BL540.0e+0070.18Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A6J1DUA71.6e-29866.79cation/H(+) antiporter 4-like OS=Momordica charantia OX=3673 GN=LOC111024501 PE=... [more]
Match NameE-valueIdentityDescription
XP_022990661.10.0e+00100.00cation/H(+) antiporter 4-like [Cucurbita maxima][more]
XP_022954106.10.0e+0095.85cation/H(+) antiporter 4-like [Cucurbita moschata][more]
KAG6601948.10.0e+0095.73Cation/H(+) antiporter 4, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7032643.10.0e+0095.60Cation/H(+) antiporter 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023550239.10.0e+0095.48cation/H(+) antiporter 4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G44900.12.0e-12835.53cation/H+ exchanger 4 [more]
AT5G22900.14.7e-12534.40cation/H+ exchanger 3 [more]
AT2G13620.16.8e-11633.38cation/hydrogen exchanger 15 [more]
AT3G44920.16.4e-11433.12cation/H+ exchanger 11 [more]
AT3G44910.16.4e-11433.29cation/H+ exchanger 12 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 60..446
e-value: 2.1E-29
score: 102.5
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 54..459
e-value: 4.1E-49
score: 169.4
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 20..790
NoneNo IPR availablePANTHERPTHR32468:SF17CATION/H(+) ANTIPORTER 4coord: 20..790

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G022060.1CmaCh04G022060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010628 positive regulation of gene expression
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016592 mediator complex
cellular_component GO:0005667 transcription regulator complex
molecular_function GO:0015299 solute:proton antiporter activity