CmaCh04G021600 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G021600
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein NRT1/ PTR FAMILY 8.3-like
LocationCma_Chr04: 15149737 .. 15154527 (+)
RNA-Seq ExpressionCmaCh04G021600
SyntenyCmaCh04G021600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCATGTGCTGATATTTATACGAGAGTCACTTTGCTTCTCCAAGTTATCCACCTTTTTATTGAAAACCTTCTGATCGGAGAAGTAGAGAAATGGGTTCTCATGAGGACGACCGAGCAATCTTGGAAGAGGGTCTCTTGCAGGTTTAATCTCTCCCTCTCTCTCTCCCTCTTTCTCTTTAGATCTTCTGCGTTTTCTGAATTTAGCTGTTTCCGGTTGTGGAATGGTCAAAGGGTGTAAATCACTTGAATCTCGTACTCATAAATACTCTGCATCCGTGATTTCATTCACTTTTTTCCTATTTTCGTTTGAATTTGTAAAGGAATATCTTCAAGATATATGGAAAAGAACATGGATGACGTTTTTGCGAATTAATTGTTTGTGTAATTAACCCATTTAAGGATTTTATGACCGATGTTTACAAGGAAAAAGATTAATGGTCAACTGTGAATGATTCGTTTTGTACAGCCAAGTCTTGATAATATTGAAGCGTTTCATGACCATTTTGAAGGCTTTTCTAATTTAGCTTGATTATGGGAGGATTGTTCTTTCCCTAAAAGTGATTTACAGTTGATAATCTGGGTGGTTGCTCAATGGTTTGATCCATTTGAACTCATGAGATGCCAATTGTGCAAGTGTACGTATACCCTTTACTTGAGTTCATTGTAACTTGAGTCTCTAACCGACAGTTCTTTGAGTTTTATCTTGTGTCTGTACCTTTGAAGCAGGCTGGATGTTGTGTGTTTAAACGTGACGTTCTTGGTTAAGTAGGAGGATTGGCCATTGATAGGTTAAAAGGTTAAATGGATTGGGATAAGAAGATATTGATGTTCAGATTTTGGGATAAGGAATGTGAAACTCGATAAGATACAACACATGTCAACTTCCTTAGCTGTTGGCACAATCATCAACTACTTTTTCTATAAGATTTCACCTCGGTTGGAGAGGGGAATGAACATTCTTTATAAGGAACGTGGAAACTTCTCCGTTCTAAAAACCTTGAGGGGAAGCTTGAAAGGGAAAGCTCAAAGAGGACAATATCTGTTAGAGGTAGGCTTGGGCTTACAAATGGTATAGGAGCCAAATACCGGGTGGTGAGGAGGTTGAGCCTTGAAGTGGGGTGGACACCAAGCGGTATGGCATGCCATTGAGGAGGCTAGGCCCCAAAGAGGAGCGGATTTGGGGTCCCACATCGATTAGAGAGGGGAACGAGTGCCAGCGAGGACATCGGGAACCAAAGGGGGTGGATTGTGAGATCCCACATCAGTTGGAGAGGGAACGAAGCATTCTTTATAAGGGTGTGAAACCTCTCTCTAGCAAACACATTTTAAAAACCTTGAGGGGAAACTCGGAAGGAAAAGCCCAAAGAGGACAATATATGCTAGCAGCGGGCTTGGACTATTACATTTGGATTGCTTATTCCAGAACTGATGGAATGAATATGCTAACCAGTTTGTAGTCCACCACATGATTGGCGTGTATAATATATAGACATCATAATTACATATGTATTATAAAGGTATTTTAGTAACGTTTTTCTCTTAACATGTCATATTACATACGTCATTTTAATATTTTTCATACTCAAGAAACGTTTAAGGGTACTTATGTTATCCATCCAACACTCCGGGGTTAACTCCTTCAAATGCTTAAAATTTTGTTTCCTTCTGTAGAATGAAAGCAATGGACTCTATACAGGCGATGGCTCAGTTGACTTCCATGGGAATCCTGTTCTCAAACAAAATACTGGAAACTGGAAAGCATGCCCATTCATTCTAGGTGATCCTCTTTCTCTTTGTGCTTTGGTAAAATTACATTAATTTTAGTACCTTAACTTAGACTTGACAATTTAAACTATGAAATCTTCCATCATTCTTCTAAAGGTATTTTTGTGTTCTTCCCATTTGGAGTTCTTTTTTCTTCCTTTTTATTCTTTACTTGACATTTCGAGCCACTTATTTGTAATACAGGGAATGAAGGCTGTGAACGATTGGCCTACTATGGGATTTCGACAAACCTTGTCACTTATCTTACTAATAAGTTACATCAAGGAAATGTTGCAGCTGCTAGAAATGTCACTACTTGGCAAGGAACTTGTTATCTTACACCCCTCATTGGAGCTATCTTGGCCGATGCTTACTGGGGAAGATATTGGACAATTGCTGCCTTTTCAACAATTTACTTCATTGTATGTTTCATCTGATGTAGATTAGAATACTAGACTTGTGTGTCAGCGTCGATATTTTCTTTTTTAGAGTGCAACTAAAGATGCAGGTTTTTCTTTACAGGGAATGTGTACGTTAACTCTCTCAGCATCGGTTCCTGCTCTAAAGCCTGCTGAATGCGTCGGGTTCATGTGCCCCCCGGCTTCTGCTGCTCAGTATATTGTATTTTTCCTTGGACTCTATCTGATTGCACTTGGGACAGGTGGAATCAAGCCATGTGTTTCATCCTTTGGAGCTGATCAGTTTGATGATTCTGATCCTGCTGAAAGGGTTAAAAAGGGCTCGTTTTTCAACTGGTTTTACTTTTCTATCAATATTGGTGCTCTTATATCAAGTAGTTTGCTAGTTTGGATACAAGACAATGCTGGGTGGGGTCTAGGATTTGGCATCCCTGCATTGTTTATGGGCCTTGCTATAATAAGTTTCTTTTCTGGCACAAAGCTCTACAGATTTCAGAAGCCGGGGGGGAGCCCAATTACGAGAATGTGCCAGGTTTTGGTTGCGTCCTTTCGTAAACGAAATCTAAAGGTTCCTGGTGACAGTAATCTTCTGTATGAGGTGCAAGATAAAAGTTCTGCCATTGAAGGAAGTCGTAAGCTCGAACACAGTAATGAACTGAGGTAACTTTCTTCACTACCTGAGATATAATCTCATAGCTGCTTTCCAAAATATTTAGAATTCACGTCGAGATTCGATTTCTAATGATTGACGAACTGATGCAGCTAACCAGTCTTCCTTTCAACATTTATGCATATGTATTGTCGACCCGATCTTCCTCGTCCCGTTTCCCAAGCAACTTCTAATATCGTGTTATTGGGATCTCCCAAATGATGATTGCAATAACAGTATTTAGCATTAGCTTCTTATATTGTAGTATTTCCTGCTGAGACATACATTTGACAGTGTTTTGATAGCTTTTCTATGCATATTTCGACCTGTTCTAATTGGTTGATATCTGGTTGGACAATTAGCAACTTTACTGTTGCACCTTGACCTCAGAAATTATGTTCGTTTGTGAGATCCTGCAATGGTTGGAGAGGGGAACGACGCATTCCTTATAAGGGTGTGGGAAACCTCTCCCTATTAGACACGTTTTAAAACTGTGAGGCTGACGGTGATACATAACGGGTCAAAGCGGACGATATTTGCTAGTAGTGGGCTTGGGTTGTTACAAATGGTATTCGAGCCAGACACCGGGCAGTGTGCTAGCGAGGACGCTGGGTCCTAAGGGAGTGGATTTTGAGATCCCACATCGATTGGAAAAGGGAACTAGCATTCCTTATAAGGGTGTGAAAACTTCTCCCTAGTAGATGTGTTTTAAAATTGTGAGGTTGACAGCGATATGTAACGGGCTAAAACGGATAATATCTGCTAGCGGTGGGCTTGGGATGTTACAAATGGTATTGGAGCCAGACATCGGGTGGTGCGCTGGCTCCCAAGAGGGGTGAATTGTGAAATCCCATATCGGATGGAGAAGGGAACGAAACATTCCTTGTTAGGGTGTGGAAACCTTTCCTTAGTAAATGCGTTTTAAAACCATAAGGTTGACTGTGATACGTGATAGACTAAAACAGACAAATATCTGAGTTGTGTTACATCATTACTTTCTGTATAGACATTCTTTGTAGGAAACGGCATCATTTTGTCTAGACATGTGCTCAGACAACTTGATTATGATTGAATTACAAGAAAACGACTTAGTGGCTATACATGTATAAAAACCGAACTGTTATTTCCTGCAGATGCCTTGACAAAGCGGCTGTCATCTCTGATGCTGAGTTGAAAAGTGGCGACTTCTCGGATCCATGGAGGCTTTGCACGGTAACACAAATCGAGGAGTTCAAGATTCTTATCCGCATGTTCCCCATATGGGCTACTGGAATTGTCTTTGCAGCCGTATACGCCCAAATGTCGACGTTATTCGTGGAGCAAGGGACAATGCTGGACAAGACCATTGGTTCTTTCCACATTCCTCCAGCCTCTCTCTCGACCTTTGATGTCGTAAGCGTCATTTTCTGGGTTCCTGTGTACGATAGATTCATAGTCCCAATCGCAAGGAAACTCACAGGAAAAGAGCGAGGATTCACCGAAATACAGCGGATGGGCATCGGCCTGTGCATATCAGTTTTTTGTATGTCAGCTGCAGCCGTGGTAGAGATCAAAAGACTGAGTCTTGCAAGAGAACTCGATTTGGTGCATAAACCAGAGGCTGTACCACTGAGCATTCTGTGGCAAATACCACAATATTTCTTGCTTGGTGCAGCAGAAGTGTTCACATTCATCGGGCAGCTCGAGTTTTTCTACGATCAATCGCCCGATGCGATGAGGAGTTTGTGCAGCGCGTTGTCTCTGTTAACCAATGCGTTGGGGAACTACCTCAGCTCATTCATTCTAACCATTGTAACTTACTTGACAACAAGAAATGGGCAGGCTGGGTGGATACCTGATAACTTGAACGAGGGGCATTTGGATTTCTTCTTCTGGATGTTGGCTGGAATGAGCTTCTTGAATTTGCTGGTTTACATTGTCTGCGCCAAACGCTACCGCCCAAAGAAGGCTTCTTCTTAA

mRNA sequence

CCCATGTGCTGATATTTATACGAGAGTCACTTTGCTTCTCCAAGTTATCCACCTTTTTATTGAAAACCTTCTGATCGGAGAAGTAGAGAAATGGGTTCTCATGAGGACGACCGAGCAATCTTGGAAGAGGGTCTCTTGCAGAATGAAAGCAATGGACTCTATACAGGCGATGGCTCAGTTGACTTCCATGGGAATCCTGTTCTCAAACAAAATACTGGAAACTGGAAAGCATGCCCATTCATTCTAGGGAATGAAGGCTGTGAACGATTGGCCTACTATGGGATTTCGACAAACCTTGTCACTTATCTTACTAATAAGTTACATCAAGGAAATGTTGCAGCTGCTAGAAATGTCACTACTTGGCAAGGAACTTGTTATCTTACACCCCTCATTGGAGCTATCTTGGCCGATGCTTACTGGGGAAGATATTGGACAATTGCTGCCTTTTCAACAATTTACTTCATTGGAATGTGTACGTTAACTCTCTCAGCATCGGTTCCTGCTCTAAAGCCTGCTGAATGCGTCGGGTTCATGTGCCCCCCGGCTTCTGCTGCTCAGTATATTGTATTTTTCCTTGGACTCTATCTGATTGCACTTGGGACAGGTGGAATCAAGCCATGTGTTTCATCCTTTGGAGCTGATCAGTTTGATGATTCTGATCCTGCTGAAAGGGTTAAAAAGGGCTCGTTTTTCAACTGGTTTTACTTTTCTATCAATATTGGTGCTCTTATATCAAGTAGTTTGCTAGTTTGGATACAAGACAATGCTGGGTGGGGTCTAGGATTTGGCATCCCTGCATTGTTTATGGGCCTTGCTATAATAAGTTTCTTTTCTGGCACAAAGCTCTACAGATTTCAGAAGCCGGGGGGGAGCCCAATTACGAGAATGTGCCAGGTTTTGGTTGCGTCCTTTCGTAAACGAAATCTAAAGGTTCCTGGTGACAGTAATCTTCTGTATGAGGTGCAAGATAAAAGTTCTGCCATTGAAGGAAGTCGTAAGCTCGAACACAGTAATGAACTGAGATGCCTTGACAAAGCGGCTGTCATCTCTGATGCTGAGTTGAAAAGTGGCGACTTCTCGGATCCATGGAGGCTTTGCACGGTAACACAAATCGAGGAGTTCAAGATTCTTATCCGCATGTTCCCCATATGGGCTACTGGAATTGTCTTTGCAGCCGTATACGCCCAAATGTCGACGTTATTCGTGGAGCAAGGGACAATGCTGGACAAGACCATTGGTTCTTTCCACATTCCTCCAGCCTCTCTCTCGACCTTTGATGTCGTAAGCGTCATTTTCTGGGTTCCTGTGTACGATAGATTCATAGTCCCAATCGCAAGGAAACTCACAGGAAAAGAGCGAGGATTCACCGAAATACAGCGGATGGGCATCGGCCTGTGCATATCAGTTTTTTGTATGTCAGCTGCAGCCGTGGTAGAGATCAAAAGACTGAGTCTTGCAAGAGAACTCGATTTGGTGCATAAACCAGAGGCTGTACCACTGAGCATTCTGTGGCAAATACCACAATATTTCTTGCTTGGTGCAGCAGAAGTGTTCACATTCATCGGGCAGCTCGAGTTTTTCTACGATCAATCGCCCGATGCGATGAGGAGTTTGTGCAGCGCGTTGTCTCTGTTAACCAATGCGTTGGGGAACTACCTCAGCTCATTCATTCTAACCATTGTAACTTACTTGACAACAAGAAATGGGCAGGCTGGGTGGATACCTGATAACTTGAACGAGGGGCATTTGGATTTCTTCTTCTGGATGTTGGCTGGAATGAGCTTCTTGAATTTGCTGGTTTACATTGTCTGCGCCAAACGCTACCGCCCAAAGAAGGCTTCTTCTTAA

Coding sequence (CDS)

ATGGGTTCTCATGAGGACGACCGAGCAATCTTGGAAGAGGGTCTCTTGCAGAATGAAAGCAATGGACTCTATACAGGCGATGGCTCAGTTGACTTCCATGGGAATCCTGTTCTCAAACAAAATACTGGAAACTGGAAAGCATGCCCATTCATTCTAGGGAATGAAGGCTGTGAACGATTGGCCTACTATGGGATTTCGACAAACCTTGTCACTTATCTTACTAATAAGTTACATCAAGGAAATGTTGCAGCTGCTAGAAATGTCACTACTTGGCAAGGAACTTGTTATCTTACACCCCTCATTGGAGCTATCTTGGCCGATGCTTACTGGGGAAGATATTGGACAATTGCTGCCTTTTCAACAATTTACTTCATTGGAATGTGTACGTTAACTCTCTCAGCATCGGTTCCTGCTCTAAAGCCTGCTGAATGCGTCGGGTTCATGTGCCCCCCGGCTTCTGCTGCTCAGTATATTGTATTTTTCCTTGGACTCTATCTGATTGCACTTGGGACAGGTGGAATCAAGCCATGTGTTTCATCCTTTGGAGCTGATCAGTTTGATGATTCTGATCCTGCTGAAAGGGTTAAAAAGGGCTCGTTTTTCAACTGGTTTTACTTTTCTATCAATATTGGTGCTCTTATATCAAGTAGTTTGCTAGTTTGGATACAAGACAATGCTGGGTGGGGTCTAGGATTTGGCATCCCTGCATTGTTTATGGGCCTTGCTATAATAAGTTTCTTTTCTGGCACAAAGCTCTACAGATTTCAGAAGCCGGGGGGGAGCCCAATTACGAGAATGTGCCAGGTTTTGGTTGCGTCCTTTCGTAAACGAAATCTAAAGGTTCCTGGTGACAGTAATCTTCTGTATGAGGTGCAAGATAAAAGTTCTGCCATTGAAGGAAGTCGTAAGCTCGAACACAGTAATGAACTGAGATGCCTTGACAAAGCGGCTGTCATCTCTGATGCTGAGTTGAAAAGTGGCGACTTCTCGGATCCATGGAGGCTTTGCACGGTAACACAAATCGAGGAGTTCAAGATTCTTATCCGCATGTTCCCCATATGGGCTACTGGAATTGTCTTTGCAGCCGTATACGCCCAAATGTCGACGTTATTCGTGGAGCAAGGGACAATGCTGGACAAGACCATTGGTTCTTTCCACATTCCTCCAGCCTCTCTCTCGACCTTTGATGTCGTAAGCGTCATTTTCTGGGTTCCTGTGTACGATAGATTCATAGTCCCAATCGCAAGGAAACTCACAGGAAAAGAGCGAGGATTCACCGAAATACAGCGGATGGGCATCGGCCTGTGCATATCAGTTTTTTGTATGTCAGCTGCAGCCGTGGTAGAGATCAAAAGACTGAGTCTTGCAAGAGAACTCGATTTGGTGCATAAACCAGAGGCTGTACCACTGAGCATTCTGTGGCAAATACCACAATATTTCTTGCTTGGTGCAGCAGAAGTGTTCACATTCATCGGGCAGCTCGAGTTTTTCTACGATCAATCGCCCGATGCGATGAGGAGTTTGTGCAGCGCGTTGTCTCTGTTAACCAATGCGTTGGGGAACTACCTCAGCTCATTCATTCTAACCATTGTAACTTACTTGACAACAAGAAATGGGCAGGCTGGGTGGATACCTGATAACTTGAACGAGGGGCATTTGGATTTCTTCTTCTGGATGTTGGCTGGAATGAGCTTCTTGAATTTGCTGGTTTACATTGTCTGCGCCAAACGCTACCGCCCAAAGAAGGCTTCTTCTTAA

Protein sequence

MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERLAYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFSTIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSSFGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMGLAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEGSRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVFAAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTGKERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYFLLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQAGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS
Homology
BLAST of CmaCh04G021600 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 7.0e-264
Identity = 442/586 (75.43%), Postives = 505/586 (86.18%), Query Frame = 0

Query: 1   MGSHEDD-RAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCER 60
           MGS E++ R ++EEGL+  E   LY  DGSVDF+GNP LK+ TGNWKACPFILGNE CER
Sbjct: 1   MGSIEEEARPLIEEGLILQEVK-LYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCER 60

Query: 61  LAYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAF 120
           LAYYGI+ NL+TYLT KLHQGNV+AA NVTTWQGTCYLTPLIGA+LADAYWGRYWTIA F
Sbjct: 61  LAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACF 120

Query: 121 STIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVS 180
           S IYFIGM  LTLSASVPALKPAEC+G  CP A+ AQY +FF GLYLIALGTGGIKPCVS
Sbjct: 121 SGIYFIGMSALTLSASVPALKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVS 180

Query: 181 SFGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFM 240
           SFGADQFDD+D  ERV+K SFFNWFYFSINIGAL+SSSLLVWIQ+N GWGLGFGIP +FM
Sbjct: 181 SFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFM 240

Query: 241 GLAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIE 300
           GLAI SFF GT LYRFQKPGGSPITR+ QV+VASFRK ++KVP D+ LLYE QDK+SAI 
Sbjct: 241 GLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIA 300

Query: 301 GSRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIV 360
           GSRK+EH+++ + LDKAAVIS+ E KSGD+S+ WRLCTVTQ+EE KILIRMFPIWA+GI+
Sbjct: 301 GSRKIEHTDDCQYLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGII 360

Query: 361 FAAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLT 420
           F+AVYAQMST+FV+QG  ++  IGSF +PPA+L TFD  SVI WVP+YDRFIVP+ARK T
Sbjct: 361 FSAVYAQMSTMFVQQGRAMNCKIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFT 420

Query: 421 GKERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQY 480
           G ++GFTEIQRMGIGL +SV CM+AAA+VEI RL +A +L LV     VP+S+LWQIPQY
Sbjct: 421 GVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQY 480

Query: 481 FLLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNG 540
           F+LGAAEVF FIGQLEFFYDQSPDAMRSLCSAL+LLTNALGNYLSS ILT+VTY TTRNG
Sbjct: 481 FILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNG 540

Query: 541 QAGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 586
           Q GWI DNLN GHLD+FFW+LAG+S +N+ VY   A RY+ KKASS
Sbjct: 541 QEGWISDNLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKKASS 585

BLAST of CmaCh04G021600 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 1.1e-221
Identity = 372/593 (62.73%), Postives = 465/593 (78.41%), Query Frame = 0

Query: 3   SHEDDRAIL--EEGLL-QNESNGLYTG------DGSVDFHGNPVLKQNTGNWKACPFILG 62
           + ED+R IL  EE LL Q E N   TG      DGS+D +GNP  K+ TGNWKACPFILG
Sbjct: 5   NEEDERRILDVEESLLTQEEVNNHLTGLSSTAEDGSIDIYGNPPSKKKTGNWKACPFILG 64

Query: 63  NEGCERLAYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRY 122
           NE CERLAYYGI+ NL+TY T++LH+ NV+AA +V  WQGTCY+TPLIGA++AD+YWGRY
Sbjct: 65  NECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYITPLIGAVIADSYWGRY 124

Query: 123 WTIAAFSTIYFIGMCTLTLSASVPALKPAECVGF---MCPPASAAQYIVFFLGLYLIALG 182
           WTIA+FS IYFIGM  LTLSAS+P LKPA C G    +C PA+  QY VFF GLYLIALG
Sbjct: 125 WTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSPATTVQYAVFFTGLYLIALG 184

Query: 183 TGGIKPCVSSFGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGL 242
           TGGIKPCVSSFGADQFDD+DP ERV+K SFFNWFYFSINIG+ ISS+LLVW+Q+N GWGL
Sbjct: 185 TGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGL 244

Query: 243 GFGIPALFMGLAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYE 302
           GF IP +FMG++I SFF GT LYRFQKPGGSPITR+CQVLVA++RK  L +P D + LYE
Sbjct: 245 GFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVAAYRKLKLNLPEDISFLYE 304

Query: 303 VQDKSSAIEGSRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRM 362
            ++K+S I GSRK++H++  + LDKAAVIS+ E KSG FS+PW+LCTVTQ+EE K LIRM
Sbjct: 305 TREKNSMIAGSRKIQHTDGYKFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRM 364

Query: 363 FPIWATGIVFAAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRF 422
           FPIWA+GIV++ +Y+Q+STLFV+QG  +++ I SF IPPAS   FD + V+  +P+YDRF
Sbjct: 365 FPIWASGIVYSVLYSQISTLFVQQGRSMNRIIRSFEIPPASFGVFDTLIVLISIPIYDRF 424

Query: 423 IVPIARKLTGKERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPL 482
           +VP  R+ TG  +G T++QRMGIGL +SV  ++AAA+VE  RL LA++         V +
Sbjct: 425 LVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVETVRLQLAQDF--------VAM 484

Query: 483 SILWQIPQYFLLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTI 542
           SI WQIPQY L+G AEVF FIG++EFFYD+SPDAMRS+CSAL+LL  A+G+YLSS ILT+
Sbjct: 485 SIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTL 544

Query: 543 VTYLTTRNGQAGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKA 584
           V Y T   G+ GW+PD+LN+GHLD+FFW+L  +  +N+ VY +   ++  KKA
Sbjct: 545 VAYFTALGGKDGWVPDDLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKKA 589

BLAST of CmaCh04G021600 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.9e-209
Identity = 353/567 (62.26%), Postives = 443/567 (78.13%), Query Frame = 0

Query: 19  ESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERLAYYGISTNLVTYLTNKLH 78
           E   +YT DG+VD H NP  K+ TGNWKAC FILGNE CERLAYYG+ TNLV YL ++L+
Sbjct: 2   EEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLN 61

Query: 79  QGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFSTIYFIGMCTLTLSASVPA 138
           QGN  AA NVT W GTCY+TPLIGA +ADAY GRYWTIA F  IY  GM  LTLSASVP 
Sbjct: 62  QGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPG 121

Query: 139 LKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSSFGADQFDDSDPAERVKKG 198
           LKP  C    C P +++Q  VFF+ LY+IALGTGGIKPCVSSFGADQFD++D  E++KK 
Sbjct: 122 LKPGNCNADTCHP-NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 181

Query: 199 SFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMGLAIISFFSGTKLYRFQKP 258
           SFFNWFYFSIN+GALI++++LVWIQ N GWG GFG+P + M +A+  FF G++ YR Q+P
Sbjct: 182 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 241

Query: 259 GGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEGSRKLEHSNELRCLDKAAV 318
           GGSP+TR+ QV+VA+FRK ++KVP D +LL+E  D  S I+GSRKL H++ L+  DKAAV
Sbjct: 242 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 301

Query: 319 ISDAE-LKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVFAAVYAQMSTLFVEQGTM 378
            S ++ +K G+  +PWRLC+VTQ+EE K +I + P+WATGIVFA VY+QMST+FV QG  
Sbjct: 302 ESQSDSIKDGEV-NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 361

Query: 379 LDKTIG-SFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTGKERGFTEIQRMGIGLC 438
           +D+ +G +F IP ASLS FD VSV+FW PVYD+FI+P+ARK T  ERGFT++QRMGIGL 
Sbjct: 362 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 421

Query: 439 ISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYFLLGAAEVFTFIGQLEF 498
           +S+F M  A V+E+ RL   +  +  +  + + +SI WQIPQY L+G AEVFTFIGQLEF
Sbjct: 422 VSIFAMITAGVLEVVRLDYVKTHN-AYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEF 481

Query: 499 FYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQAGWIPDNLNEGHLDFF 558
           FYDQ+PDAMRSLCSALSL T ALGNYLS+ ++T+V  +T +NG+ GWIPDNLN GHLD+F
Sbjct: 482 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYF 541

Query: 559 FWMLAGMSFLNLLVYIVCAKRYRPKKA 584
           F++LA +SFLN LVY+  +KRY+ KKA
Sbjct: 542 FYLLATLSFLNFLVYLWISKRYKYKKA 565

BLAST of CmaCh04G021600 vs. ExPASy Swiss-Prot
Match: Q84WG0 (Protein NRT1/ PTR FAMILY 8.4 OS=Arabidopsis thaliana OX=3702 GN=NPF8.4 PE=2 SV=2)

HSP 1 Score: 719.5 bits (1856), Expect = 3.0e-206
Identity = 354/585 (60.51%), Postives = 438/585 (74.87%), Query Frame = 0

Query: 1   MGSHEDDRAIL--EEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCE 60
           M S +++R++L  EE L+Q E   LY  DGS+D HGNP LKQ TGNWKACPFI  NE CE
Sbjct: 1   MASIDEERSLLEVEESLIQEEVK-LYAEDGSIDIHGNPPLKQTTGNWKACPFIFANECCE 60

Query: 61  RLAYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAA 120
           RLAYYGI+ NL+TY TN+LH+ NV+AAR+V TWQGTCY+TPLIGA++ADAYWGRYWTIA 
Sbjct: 61  RLAYYGIAKNLITYFTNELHETNVSAARHVMTWQGTCYITPLIGALIADAYWGRYWTIAC 120

Query: 121 FSTIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCV 180
           FS IYF GM  LTLSASVP LKPAEC+G +CPPA+  Q  V F GLYLIALGTGGIKPCV
Sbjct: 121 FSAIYFTGMVALTLSASVPGLKPAECIGSLCPPATMVQSTVLFSGLYLIALGTGGIKPCV 180

Query: 181 SSFGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALF 240
           SSFGADQFD +DP+ERV+K SFFNWFYF+INIGA +SS++LVWIQ+N GW LGF IP +F
Sbjct: 181 SSFGADQFDKTDPSERVRKASFFNWFYFTINIGAFVSSTVLVWIQENYGWELGFLIPTVF 240

Query: 241 MGLAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAI 300
           MGLA +SFF GT LYRFQKP GSPIT +CQVLVA++RK NLKVP DS       D+  A 
Sbjct: 241 MGLATMSFFFGTPLYRFQKPRGSPITSVCQVLVAAYRKSNLKVPEDST------DEGDA- 300

Query: 301 EGSRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGI 360
                                          ++PW+LCTVTQ+EE KIL+R+ PIWA+GI
Sbjct: 301 ------------------------------NTNPWKLCTVTQVEEVKILLRLVPIWASGI 360

Query: 361 VFAAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKL 420
           +F+ +++Q+ TLFV+QG  + +TIG F IPPA+L  FD  SV+  VP+YDR IVP+ R+ 
Sbjct: 361 IFSVLHSQIYTLFVQQGRCMKRTIGLFEIPPATLGMFDTASVLISVPIYDRVIVPLVRRF 420

Query: 421 TGKERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQ 480
           TG  +GFTE+QRMGIGL +SV  ++ AA+VE  RL LAR+LDLV   + VPL+I WQIPQ
Sbjct: 421 TGLAKGFTELQRMGIGLFVSVLSLTFAAIVETVRLQLARDLDLVESGDIVPLNIFWQIPQ 480

Query: 481 YFLLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRN 540
           YFL+G A VF F+G++EFFY+QSPD+MRSLCSA +LLT  LGNYLSS I+T+V YL   +
Sbjct: 481 YFLMGTAGVFFFVGRIEFFYEQSPDSMRSLCSAWALLTTTLGNYLSSLIITLVAYL---S 540

Query: 541 GQAGWIP-DNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKK 583
           G+  WIP DN+N GHLD+FFW+L  +  +N+ V++  + +Y   K
Sbjct: 541 GKDCWIPSDNINNGHLDYFFWLLVSLGSVNIPVFVFFSVKYTHMK 544

BLAST of CmaCh04G021600 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 3.1e-203
Identity = 345/567 (60.85%), Postives = 430/567 (75.84%), Query Frame = 0

Query: 19  ESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERLAYYGISTNLVTYLTNKLH 78
           +   +YT DG++D H  P  K  TG WKAC FILG E CERLAYYG+STNL+ YL  +++
Sbjct: 3   DDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMN 62

Query: 79  QGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFSTIYFIGMCTLTLSASVPA 138
             NV+A+++V+ W GTCY TPLIGA +ADAY GRYWTIA+F  IY  GM  LT+SASVP 
Sbjct: 63  MENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPG 122

Query: 139 LKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSSFGADQFDDSDPAERVKKG 198
           L P  C G  C  A+A Q  + F+ LYLIALGTGGIKPCVSSFGADQFDD+D  E+  K 
Sbjct: 123 LTPT-CSGETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKS 182

Query: 199 SFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMGLAIISFFSGTKLYRFQKP 258
           SFFNWFYF IN+GA+I+SS+LVWIQ N GWG G G+P + M +A++ FF+G+  YR QKP
Sbjct: 183 SFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKP 242

Query: 259 GGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEGSRKLEHSNELRCLDKAAV 318
           GGSP+TRM QV+VAS RK  +K+P D +LLYE QD  S+I GSRKLEH+  L   DKAAV
Sbjct: 243 GGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAV 302

Query: 319 ISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVFAAVYAQMSTLFVEQGTML 378
            ++++ K    S  W+LCTVTQ+EE K LIR+ PIWATGIVFA+VY+QM T+FV QG  L
Sbjct: 303 ETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTL 362

Query: 379 DKTIG-SFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTGKERGFTEIQRMGIGLCI 438
           D+ +G +F IP ASLS FD +SV+FW PVYD+ IVP ARK TG ERGFT++QR+GIGL I
Sbjct: 363 DQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVI 422

Query: 439 SVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYFLLGAAEVFTFIGQLEFF 498
           S+F M +A ++E+ RL+  +  +L +  E +P++I WQ+PQYFL+G AEVFTFIGQLEFF
Sbjct: 423 SIFSMVSAGILEVARLNYVQTHNL-YNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFF 482

Query: 499 YDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQAGWIPDNLNEGHLDFFF 558
           YDQ+PDAMRSLCSALSL   A GNYLS+F++T+VT +T   G+ GWI  NLN GHLD+FF
Sbjct: 483 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFF 542

Query: 559 WMLAGMSFLNLLVYIVCAKRYRPKKAS 585
           W+LAG+SFLN LVY+  AK Y  KK +
Sbjct: 543 WLLAGLSFLNFLVYLWIAKWYTYKKTT 566

BLAST of CmaCh04G021600 vs. ExPASy TrEMBL
Match: A0A6J1JTA6 (protein NRT1/ PTR FAMILY 8.3-like OS=Cucurbita maxima OX=3661 GN=LOC111487649 PE=3 SV=1)

HSP 1 Score: 1184.1 bits (3062), Expect = 0.0e+00
Identity = 585/585 (100.00%), Postives = 585/585 (100.00%), Query Frame = 0

Query: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60
           MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL
Sbjct: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60

Query: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120
           AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS
Sbjct: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120

Query: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180
           TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS
Sbjct: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180

Query: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240
           FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG
Sbjct: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240

Query: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300
           LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG
Sbjct: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300

Query: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360
           SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF
Sbjct: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360

Query: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420
           AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG
Sbjct: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420

Query: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480
           KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF
Sbjct: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480

Query: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540
           LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ
Sbjct: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540

Query: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 586
           AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS
Sbjct: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 585

BLAST of CmaCh04G021600 vs. ExPASy TrEMBL
Match: A0A6J1HCP4 (protein NRT1/ PTR FAMILY 8.3-like OS=Cucurbita moschata OX=3662 GN=LOC111462778 PE=3 SV=1)

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 584/585 (99.83%), Postives = 584/585 (99.83%), Query Frame = 0

Query: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60
           MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL
Sbjct: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60

Query: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120
           AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS
Sbjct: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120

Query: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180
           TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS
Sbjct: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180

Query: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240
           FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG
Sbjct: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240

Query: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300
           LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG
Sbjct: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300

Query: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360
           SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF
Sbjct: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360

Query: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420
           AAVYAQMSTLFVEQGTMLDKTIGSF IPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG
Sbjct: 361 AAVYAQMSTLFVEQGTMLDKTIGSFQIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420

Query: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480
           KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF
Sbjct: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480

Query: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540
           LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ
Sbjct: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540

Query: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 586
           AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS
Sbjct: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 585

BLAST of CmaCh04G021600 vs. ExPASy TrEMBL
Match: A0A5A7VM51 (Protein NRT1/ PTR FAMILY 8.3 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold174G00520 PE=3 SV=1)

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 559/584 (95.72%), Postives = 573/584 (98.12%), Query Frame = 0

Query: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60
           MGS EDDRAILEEGLLQNESN LYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL
Sbjct: 1   MGSQEDDRAILEEGLLQNESNALYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60

Query: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120
           AYYGISTNLVTYLTNKLHQGNV+AARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS
Sbjct: 61  AYYGISTNLVTYLTNKLHQGNVSAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120

Query: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180
           TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQY+VFFLGLYLIALGTGGIKPCVSS
Sbjct: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYMVFFLGLYLIALGTGGIKPCVSS 180

Query: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240
           FGADQFDD+DPAERVKKGSFFNWFYFSINIGALISSS LVWIQDNAGWGLGFGIPA+FMG
Sbjct: 181 FGADQFDDTDPAERVKKGSFFNWFYFSINIGALISSSFLVWIQDNAGWGLGFGIPAVFMG 240

Query: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300
           LAI+SFFSGTKLYRFQKPGGSPITRMCQVLVASF KRNL+VPGDSNLLYEVQDKSSAIEG
Sbjct: 241 LAIVSFFSGTKLYRFQKPGGSPITRMCQVLVASFHKRNLRVPGDSNLLYEVQDKSSAIEG 300

Query: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360
           SRKLEHS+ELRCLDKAAVISDAELKSGD+S+PWRLCTVTQ+EEFKILIRMFPIWATGIVF
Sbjct: 301 SRKLEHSDELRCLDKAAVISDAELKSGDYSNPWRLCTVTQVEEFKILIRMFPIWATGIVF 360

Query: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420
           AAVYAQMSTLFVEQGTMLDKTIGSF IPPASLSTFDVVSVIFWVPVYDRFIVPIA+K TG
Sbjct: 361 AAVYAQMSTLFVEQGTMLDKTIGSFRIPPASLSTFDVVSVIFWVPVYDRFIVPIAKKFTG 420

Query: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480
           KERGFTEIQRMGIGL ISV CMSAAAVVEIKRL LARELDLVHKPEAVPLSILWQIPQYF
Sbjct: 421 KERGFTEIQRMGIGLFISVLCMSAAAVVEIKRLELARELDLVHKPEAVPLSILWQIPQYF 480

Query: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540
           LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLT ALGNYLSSFILTIVTYLTTRNGQ
Sbjct: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTTALGNYLSSFILTIVTYLTTRNGQ 540

Query: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKAS 585
           +GWIPDNLNEGHLDFFFW+LAG+SFLNLLVY VCAKRYRPKKAS
Sbjct: 541 SGWIPDNLNEGHLDFFFWLLAGLSFLNLLVYTVCAKRYRPKKAS 584

BLAST of CmaCh04G021600 vs. ExPASy TrEMBL
Match: A0A1S3CTB9 (protein NRT1/ PTR FAMILY 8.3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103504109 PE=3 SV=1)

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 558/584 (95.55%), Postives = 573/584 (98.12%), Query Frame = 0

Query: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60
           MGS EDDRAILEEGLLQNESN L+TGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL
Sbjct: 1   MGSQEDDRAILEEGLLQNESNALFTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60

Query: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120
           AYYGISTNLVTYLTNKLHQGNV+AARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS
Sbjct: 61  AYYGISTNLVTYLTNKLHQGNVSAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120

Query: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180
           TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQY+VFFLGLYLIALGTGGIKPCVSS
Sbjct: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYMVFFLGLYLIALGTGGIKPCVSS 180

Query: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240
           FGADQFDD+DPAERVKKGSFFNWFYFSINIGALISSS LVWIQDNAGWGLGFGIPA+FMG
Sbjct: 181 FGADQFDDTDPAERVKKGSFFNWFYFSINIGALISSSFLVWIQDNAGWGLGFGIPAVFMG 240

Query: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300
           LAI+SFFSGTKLYRFQKPGGSPITRMCQVLVASF KRNL+VPGDSNLLYEVQDKSSAIEG
Sbjct: 241 LAIVSFFSGTKLYRFQKPGGSPITRMCQVLVASFHKRNLRVPGDSNLLYEVQDKSSAIEG 300

Query: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360
           SRKLEHS+ELRCLDKAAVISDAELKSGD+S+PWRLCTVTQ+EEFKILIRMFPIWATGIVF
Sbjct: 301 SRKLEHSDELRCLDKAAVISDAELKSGDYSNPWRLCTVTQVEEFKILIRMFPIWATGIVF 360

Query: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420
           AAVYAQMSTLFVEQGTMLDKTIGSF IPPASLSTFDVVSVIFWVPVYDRFIVPIA+K TG
Sbjct: 361 AAVYAQMSTLFVEQGTMLDKTIGSFRIPPASLSTFDVVSVIFWVPVYDRFIVPIAKKFTG 420

Query: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480
           KERGFTEIQRMGIGL ISV CMSAAAVVEIKRL LARELDLVHKPEAVPLSILWQIPQYF
Sbjct: 421 KERGFTEIQRMGIGLFISVLCMSAAAVVEIKRLELARELDLVHKPEAVPLSILWQIPQYF 480

Query: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540
           LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLT ALGNYLSSFILTIVTYLTTRNGQ
Sbjct: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTTALGNYLSSFILTIVTYLTTRNGQ 540

Query: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKAS 585
           +GWIPDNLNEGHLDFFFW+LAG+SFLNLLVY VCAKRYRPKKAS
Sbjct: 541 SGWIPDNLNEGHLDFFFWLLAGLSFLNLLVYTVCAKRYRPKKAS 584

BLAST of CmaCh04G021600 vs. ExPASy TrEMBL
Match: A0A0A0KMV5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G106020 PE=3 SV=1)

HSP 1 Score: 1134.4 bits (2933), Expect = 0.0e+00
Identity = 558/584 (95.55%), Postives = 570/584 (97.60%), Query Frame = 0

Query: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60
           MGS EDDRAILEEGLLQNES+ LYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL
Sbjct: 1   MGSQEDDRAILEEGLLQNESSTLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60

Query: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120
           AYYGISTNLVTYLTNKLHQGNV+AARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS
Sbjct: 61  AYYGISTNLVTYLTNKLHQGNVSAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120

Query: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180
           TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQY+VFFLGLYLIALGTGGIKPCVSS
Sbjct: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYMVFFLGLYLIALGTGGIKPCVSS 180

Query: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240
           FGADQFDD+DPAERVKKGSFFNWFYFSINIGALISSS LVWIQDNAGWGLGFGIPA+FMG
Sbjct: 181 FGADQFDDTDPAERVKKGSFFNWFYFSINIGALISSSFLVWIQDNAGWGLGFGIPAVFMG 240

Query: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300
           LAI+SFFSGTKLYRFQKPGGSPITRMCQVLVASF KRNL VP DSNLLYEVQDKSSAIEG
Sbjct: 241 LAIVSFFSGTKLYRFQKPGGSPITRMCQVLVASFHKRNLTVPSDSNLLYEVQDKSSAIEG 300

Query: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360
           SRKLEHS+ELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF
Sbjct: 301 SRKLEHSDELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360

Query: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420
           AAVYAQMSTLFVEQGTMLDKTIGSF IPPASLSTFDVVSVIFWVPVYDRFIVPIA+K TG
Sbjct: 361 AAVYAQMSTLFVEQGTMLDKTIGSFRIPPASLSTFDVVSVIFWVPVYDRFIVPIAKKFTG 420

Query: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480
           KERGFTEIQRMGIGL ISV CMSAAAVVEIKRL LARELDLVHKPEAVPLSILWQIPQYF
Sbjct: 421 KERGFTEIQRMGIGLFISVLCMSAAAVVEIKRLELARELDLVHKPEAVPLSILWQIPQYF 480

Query: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540
           LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLT ALGNYLSSFILTIVTYLTTRNGQ
Sbjct: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTTALGNYLSSFILTIVTYLTTRNGQ 540

Query: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKAS 585
           +GWIPDNLNEGHLD FFW+LAG+SFLNLLVY VCAKRYRPKKA+
Sbjct: 541 SGWIPDNLNEGHLDLFFWLLAGLSFLNLLVYTVCAKRYRPKKAT 584

BLAST of CmaCh04G021600 vs. NCBI nr
Match: XP_022990899.1 (protein NRT1/ PTR FAMILY 8.3-like [Cucurbita maxima])

HSP 1 Score: 1184.1 bits (3062), Expect = 0.0e+00
Identity = 585/585 (100.00%), Postives = 585/585 (100.00%), Query Frame = 0

Query: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60
           MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL
Sbjct: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60

Query: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120
           AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS
Sbjct: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120

Query: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180
           TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS
Sbjct: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180

Query: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240
           FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG
Sbjct: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240

Query: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300
           LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG
Sbjct: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300

Query: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360
           SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF
Sbjct: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360

Query: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420
           AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG
Sbjct: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420

Query: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480
           KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF
Sbjct: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480

Query: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540
           LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ
Sbjct: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540

Query: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 586
           AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS
Sbjct: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 585

BLAST of CmaCh04G021600 vs. NCBI nr
Match: XP_022962286.1 (protein NRT1/ PTR FAMILY 8.3-like [Cucurbita moschata] >XP_023543658.1 protein NRT1/ PTR FAMILY 8.3-like [Cucurbita pepo subsp. pepo] >KAG6601908.1 Protein NRT1/ PTR FAMILY 8.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 584/585 (99.83%), Postives = 584/585 (99.83%), Query Frame = 0

Query: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60
           MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL
Sbjct: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60

Query: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120
           AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS
Sbjct: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120

Query: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180
           TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS
Sbjct: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180

Query: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240
           FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG
Sbjct: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240

Query: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300
           LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG
Sbjct: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300

Query: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360
           SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF
Sbjct: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360

Query: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420
           AAVYAQMSTLFVEQGTMLDKTIGSF IPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG
Sbjct: 361 AAVYAQMSTLFVEQGTMLDKTIGSFQIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420

Query: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480
           KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF
Sbjct: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480

Query: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540
           LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ
Sbjct: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540

Query: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 586
           AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS
Sbjct: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 585

BLAST of CmaCh04G021600 vs. NCBI nr
Match: KAG7032606.1 (Protein NRT1/ PTR FAMILY 8.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 567/568 (99.82%), Postives = 567/568 (99.82%), Query Frame = 0

Query: 18  NESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERLAYYGISTNLVTYLTNKL 77
           NESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERLAYYGISTNLVTYLTNKL
Sbjct: 1   NESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERLAYYGISTNLVTYLTNKL 60

Query: 78  HQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFSTIYFIGMCTLTLSASVP 137
           HQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFSTIYFIGMCTLTLSASVP
Sbjct: 61  HQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFSTIYFIGMCTLTLSASVP 120

Query: 138 ALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSSFGADQFDDSDPAERVKK 197
           ALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSSFGADQFDDSDPAERVKK
Sbjct: 121 ALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSSFGADQFDDSDPAERVKK 180

Query: 198 GSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMGLAIISFFSGTKLYRFQK 257
           GSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMGLAIISFFSGTKLYRFQK
Sbjct: 181 GSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMGLAIISFFSGTKLYRFQK 240

Query: 258 PGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEGSRKLEHSNELRCLDKAA 317
           PGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEGSRKLEHSNELRCLDKAA
Sbjct: 241 PGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEGSRKLEHSNELRCLDKAA 300

Query: 318 VISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVFAAVYAQMSTLFVEQGTM 377
           VISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVFAAVYAQMSTLFVEQGTM
Sbjct: 301 VISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVFAAVYAQMSTLFVEQGTM 360

Query: 378 LDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTGKERGFTEIQRMGIGLCI 437
           LDKTIGSF IPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTGKERGFTEIQRMGIGLCI
Sbjct: 361 LDKTIGSFQIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTGKERGFTEIQRMGIGLCI 420

Query: 438 SVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYFLLGAAEVFTFIGQLEFF 497
           SVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYFLLGAAEVFTFIGQLEFF
Sbjct: 421 SVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYFLLGAAEVFTFIGQLEFF 480

Query: 498 YDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQAGWIPDNLNEGHLDFFF 557
           YDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQAGWIPDNLNEGHLDFFF
Sbjct: 481 YDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQAGWIPDNLNEGHLDFFF 540

Query: 558 WMLAGMSFLNLLVYIVCAKRYRPKKASS 586
           WMLAGMSFLNLLVYIVCAKRYRPKKASS
Sbjct: 541 WMLAGMSFLNLLVYIVCAKRYRPKKASS 568

BLAST of CmaCh04G021600 vs. NCBI nr
Match: XP_038875238.1 (protein NRT1/ PTR FAMILY 8.3 [Benincasa hispida])

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 561/584 (96.06%), Postives = 574/584 (98.29%), Query Frame = 0

Query: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60
           MGS EDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL
Sbjct: 1   MGSQEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60

Query: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120
           AYYGISTNLVTYLTNKLHQGNV+AARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS
Sbjct: 61  AYYGISTNLVTYLTNKLHQGNVSAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120

Query: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180
           TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQY+VFFLGLYLIALGTGGIKPCVSS
Sbjct: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYMVFFLGLYLIALGTGGIKPCVSS 180

Query: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240
           FGADQFDD+DPAERVKKGSFFNWFYFSINIGALISSS LVWIQDNAGWGLGFGIPALFMG
Sbjct: 181 FGADQFDDTDPAERVKKGSFFNWFYFSINIGALISSSFLVWIQDNAGWGLGFGIPALFMG 240

Query: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300
           LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASF KR+L+VP DSNLLYEV+DKSSAIEG
Sbjct: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFHKRDLRVPADSNLLYEVRDKSSAIEG 300

Query: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360
           SRKLEHS+ELRCLDKAAVISDAELKSGDFSDPWRLCTVTQ+EEFKILIRMFPIWATGIVF
Sbjct: 301 SRKLEHSDELRCLDKAAVISDAELKSGDFSDPWRLCTVTQVEEFKILIRMFPIWATGIVF 360

Query: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420
           AAVYAQMSTLFVEQGTML+KTIGSF IPPASLSTFDVVSVIFWVPVYDRFIVPIARK+TG
Sbjct: 361 AAVYAQMSTLFVEQGTMLNKTIGSFRIPPASLSTFDVVSVIFWVPVYDRFIVPIARKVTG 420

Query: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480
           KERGFTEIQRMGIGL ISV CMSAAA+VEIKRL LARELDLVHKPEAVPLSILWQIPQYF
Sbjct: 421 KERGFTEIQRMGIGLFISVLCMSAAAMVEIKRLELARELDLVHKPEAVPLSILWQIPQYF 480

Query: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540
           LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLT ALGNYLSSFILTIVTYLTTRNGQ
Sbjct: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTTALGNYLSSFILTIVTYLTTRNGQ 540

Query: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKAS 585
            GWIPDNLNEGHLDFFFW+LAG+SFLNLLVYIVCAKRYRPKKAS
Sbjct: 541 PGWIPDNLNEGHLDFFFWLLAGLSFLNLLVYIVCAKRYRPKKAS 584

BLAST of CmaCh04G021600 vs. NCBI nr
Match: KAA0067586.1 (protein NRT1/ PTR FAMILY 8.3 isoform X2 [Cucumis melo var. makuwa] >TYJ97173.1 protein NRT1/ PTR FAMILY 8.3 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 559/584 (95.72%), Postives = 573/584 (98.12%), Query Frame = 0

Query: 1   MGSHEDDRAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60
           MGS EDDRAILEEGLLQNESN LYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL
Sbjct: 1   MGSQEDDRAILEEGLLQNESNALYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERL 60

Query: 61  AYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120
           AYYGISTNLVTYLTNKLHQGNV+AARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS
Sbjct: 61  AYYGISTNLVTYLTNKLHQGNVSAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFS 120

Query: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSS 180
           TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQY+VFFLGLYLIALGTGGIKPCVSS
Sbjct: 121 TIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYMVFFLGLYLIALGTGGIKPCVSS 180

Query: 181 FGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMG 240
           FGADQFDD+DPAERVKKGSFFNWFYFSINIGALISSS LVWIQDNAGWGLGFGIPA+FMG
Sbjct: 181 FGADQFDDTDPAERVKKGSFFNWFYFSINIGALISSSFLVWIQDNAGWGLGFGIPAVFMG 240

Query: 241 LAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEG 300
           LAI+SFFSGTKLYRFQKPGGSPITRMCQVLVASF KRNL+VPGDSNLLYEVQDKSSAIEG
Sbjct: 241 LAIVSFFSGTKLYRFQKPGGSPITRMCQVLVASFHKRNLRVPGDSNLLYEVQDKSSAIEG 300

Query: 301 SRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVF 360
           SRKLEHS+ELRCLDKAAVISDAELKSGD+S+PWRLCTVTQ+EEFKILIRMFPIWATGIVF
Sbjct: 301 SRKLEHSDELRCLDKAAVISDAELKSGDYSNPWRLCTVTQVEEFKILIRMFPIWATGIVF 360

Query: 361 AAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTG 420
           AAVYAQMSTLFVEQGTMLDKTIGSF IPPASLSTFDVVSVIFWVPVYDRFIVPIA+K TG
Sbjct: 361 AAVYAQMSTLFVEQGTMLDKTIGSFRIPPASLSTFDVVSVIFWVPVYDRFIVPIAKKFTG 420

Query: 421 KERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYF 480
           KERGFTEIQRMGIGL ISV CMSAAAVVEIKRL LARELDLVHKPEAVPLSILWQIPQYF
Sbjct: 421 KERGFTEIQRMGIGLFISVLCMSAAAVVEIKRLELARELDLVHKPEAVPLSILWQIPQYF 480

Query: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQ 540
           LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLT ALGNYLSSFILTIVTYLTTRNGQ
Sbjct: 481 LLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTTALGNYLSSFILTIVTYLTTRNGQ 540

Query: 541 AGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKAS 585
           +GWIPDNLNEGHLDFFFW+LAG+SFLNLLVY VCAKRYRPKKAS
Sbjct: 541 SGWIPDNLNEGHLDFFFWLLAGLSFLNLLVYTVCAKRYRPKKAS 584

BLAST of CmaCh04G021600 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 911.0 bits (2353), Expect = 5.0e-265
Identity = 442/586 (75.43%), Postives = 505/586 (86.18%), Query Frame = 0

Query: 1   MGSHEDD-RAILEEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCER 60
           MGS E++ R ++EEGL+  E   LY  DGSVDF+GNP LK+ TGNWKACPFILGNE CER
Sbjct: 1   MGSIEEEARPLIEEGLILQEVK-LYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCER 60

Query: 61  LAYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAF 120
           LAYYGI+ NL+TYLT KLHQGNV+AA NVTTWQGTCYLTPLIGA+LADAYWGRYWTIA F
Sbjct: 61  LAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACF 120

Query: 121 STIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVS 180
           S IYFIGM  LTLSASVPALKPAEC+G  CP A+ AQY +FF GLYLIALGTGGIKPCVS
Sbjct: 121 SGIYFIGMSALTLSASVPALKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVS 180

Query: 181 SFGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFM 240
           SFGADQFDD+D  ERV+K SFFNWFYFSINIGAL+SSSLLVWIQ+N GWGLGFGIP +FM
Sbjct: 181 SFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFM 240

Query: 241 GLAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIE 300
           GLAI SFF GT LYRFQKPGGSPITR+ QV+VASFRK ++KVP D+ LLYE QDK+SAI 
Sbjct: 241 GLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIA 300

Query: 301 GSRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIV 360
           GSRK+EH+++ + LDKAAVIS+ E KSGD+S+ WRLCTVTQ+EE KILIRMFPIWA+GI+
Sbjct: 301 GSRKIEHTDDCQYLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGII 360

Query: 361 FAAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLT 420
           F+AVYAQMST+FV+QG  ++  IGSF +PPA+L TFD  SVI WVP+YDRFIVP+ARK T
Sbjct: 361 FSAVYAQMSTMFVQQGRAMNCKIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFT 420

Query: 421 GKERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQY 480
           G ++GFTEIQRMGIGL +SV CM+AAA+VEI RL +A +L LV     VP+S+LWQIPQY
Sbjct: 421 GVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQY 480

Query: 481 FLLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNG 540
           F+LGAAEVF FIGQLEFFYDQSPDAMRSLCSAL+LLTNALGNYLSS ILT+VTY TTRNG
Sbjct: 481 FILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNG 540

Query: 541 QAGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKASS 586
           Q GWI DNLN GHLD+FFW+LAG+S +N+ VY   A RY+ KKASS
Sbjct: 541 QEGWISDNLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKKASS 585

BLAST of CmaCh04G021600 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 770.8 bits (1989), Expect = 8.0e-223
Identity = 372/593 (62.73%), Postives = 465/593 (78.41%), Query Frame = 0

Query: 3   SHEDDRAIL--EEGLL-QNESNGLYTG------DGSVDFHGNPVLKQNTGNWKACPFILG 62
           + ED+R IL  EE LL Q E N   TG      DGS+D +GNP  K+ TGNWKACPFILG
Sbjct: 5   NEEDERRILDVEESLLTQEEVNNHLTGLSSTAEDGSIDIYGNPPSKKKTGNWKACPFILG 64

Query: 63  NEGCERLAYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRY 122
           NE CERLAYYGI+ NL+TY T++LH+ NV+AA +V  WQGTCY+TPLIGA++AD+YWGRY
Sbjct: 65  NECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYITPLIGAVIADSYWGRY 124

Query: 123 WTIAAFSTIYFIGMCTLTLSASVPALKPAECVGF---MCPPASAAQYIVFFLGLYLIALG 182
           WTIA+FS IYFIGM  LTLSAS+P LKPA C G    +C PA+  QY VFF GLYLIALG
Sbjct: 125 WTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSPATTVQYAVFFTGLYLIALG 184

Query: 183 TGGIKPCVSSFGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGL 242
           TGGIKPCVSSFGADQFDD+DP ERV+K SFFNWFYFSINIG+ ISS+LLVW+Q+N GWGL
Sbjct: 185 TGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGL 244

Query: 243 GFGIPALFMGLAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYE 302
           GF IP +FMG++I SFF GT LYRFQKPGGSPITR+CQVLVA++RK  L +P D + LYE
Sbjct: 245 GFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVAAYRKLKLNLPEDISFLYE 304

Query: 303 VQDKSSAIEGSRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRM 362
            ++K+S I GSRK++H++  + LDKAAVIS+ E KSG FS+PW+LCTVTQ+EE K LIRM
Sbjct: 305 TREKNSMIAGSRKIQHTDGYKFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRM 364

Query: 363 FPIWATGIVFAAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRF 422
           FPIWA+GIV++ +Y+Q+STLFV+QG  +++ I SF IPPAS   FD + V+  +P+YDRF
Sbjct: 365 FPIWASGIVYSVLYSQISTLFVQQGRSMNRIIRSFEIPPASFGVFDTLIVLISIPIYDRF 424

Query: 423 IVPIARKLTGKERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPL 482
           +VP  R+ TG  +G T++QRMGIGL +SV  ++AAA+VE  RL LA++         V +
Sbjct: 425 LVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVETVRLQLAQDF--------VAM 484

Query: 483 SILWQIPQYFLLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTI 542
           SI WQIPQY L+G AEVF FIG++EFFYD+SPDAMRS+CSAL+LL  A+G+YLSS ILT+
Sbjct: 485 SIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTL 544

Query: 543 VTYLTTRNGQAGWIPDNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKKA 584
           V Y T   G+ GW+PD+LN+GHLD+FFW+L  +  +N+ VY +   ++  KKA
Sbjct: 545 VAYFTALGGKDGWVPDDLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKKA 589

BLAST of CmaCh04G021600 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 729.6 bits (1882), Expect = 2.0e-210
Identity = 353/567 (62.26%), Postives = 443/567 (78.13%), Query Frame = 0

Query: 19  ESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERLAYYGISTNLVTYLTNKLH 78
           E   +YT DG+VD H NP  K+ TGNWKAC FILGNE CERLAYYG+ TNLV YL ++L+
Sbjct: 2   EEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLN 61

Query: 79  QGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFSTIYFIGMCTLTLSASVPA 138
           QGN  AA NVT W GTCY+TPLIGA +ADAY GRYWTIA F  IY  GM  LTLSASVP 
Sbjct: 62  QGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPG 121

Query: 139 LKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSSFGADQFDDSDPAERVKKG 198
           LKP  C    C P +++Q  VFF+ LY+IALGTGGIKPCVSSFGADQFD++D  E++KK 
Sbjct: 122 LKPGNCNADTCHP-NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 181

Query: 199 SFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMGLAIISFFSGTKLYRFQKP 258
           SFFNWFYFSIN+GALI++++LVWIQ N GWG GFG+P + M +A+  FF G++ YR Q+P
Sbjct: 182 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 241

Query: 259 GGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEGSRKLEHSNELRCLDKAAV 318
           GGSP+TR+ QV+VA+FRK ++KVP D +LL+E  D  S I+GSRKL H++ L+  DKAAV
Sbjct: 242 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 301

Query: 319 ISDAE-LKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVFAAVYAQMSTLFVEQGTM 378
            S ++ +K G+  +PWRLC+VTQ+EE K +I + P+WATGIVFA VY+QMST+FV QG  
Sbjct: 302 ESQSDSIKDGEV-NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 361

Query: 379 LDKTIG-SFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTGKERGFTEIQRMGIGLC 438
           +D+ +G +F IP ASLS FD VSV+FW PVYD+FI+P+ARK T  ERGFT++QRMGIGL 
Sbjct: 362 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 421

Query: 439 ISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYFLLGAAEVFTFIGQLEF 498
           +S+F M  A V+E+ RL   +  +  +  + + +SI WQIPQY L+G AEVFTFIGQLEF
Sbjct: 422 VSIFAMITAGVLEVVRLDYVKTHN-AYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEF 481

Query: 499 FYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQAGWIPDNLNEGHLDFF 558
           FYDQ+PDAMRSLCSALSL T ALGNYLS+ ++T+V  +T +NG+ GWIPDNLN GHLD+F
Sbjct: 482 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYF 541

Query: 559 FWMLAGMSFLNLLVYIVCAKRYRPKKA 584
           F++LA +SFLN LVY+  +KRY+ KKA
Sbjct: 542 FYLLATLSFLNFLVYLWISKRYKYKKA 565

BLAST of CmaCh04G021600 vs. TAIR 10
Match: AT2G02020.1 (Major facilitator superfamily protein )

HSP 1 Score: 719.5 bits (1856), Expect = 2.1e-207
Identity = 354/585 (60.51%), Postives = 438/585 (74.87%), Query Frame = 0

Query: 1   MGSHEDDRAIL--EEGLLQNESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCE 60
           M S +++R++L  EE L+Q E   LY  DGS+D HGNP LKQ TGNWKACPFI  NE CE
Sbjct: 1   MASIDEERSLLEVEESLIQEEVK-LYAEDGSIDIHGNPPLKQTTGNWKACPFIFANECCE 60

Query: 61  RLAYYGISTNLVTYLTNKLHQGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAA 120
           RLAYYGI+ NL+TY TN+LH+ NV+AAR+V TWQGTCY+TPLIGA++ADAYWGRYWTIA 
Sbjct: 61  RLAYYGIAKNLITYFTNELHETNVSAARHVMTWQGTCYITPLIGALIADAYWGRYWTIAC 120

Query: 121 FSTIYFIGMCTLTLSASVPALKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCV 180
           FS IYF GM  LTLSASVP LKPAEC+G +CPPA+  Q  V F GLYLIALGTGGIKPCV
Sbjct: 121 FSAIYFTGMVALTLSASVPGLKPAECIGSLCPPATMVQSTVLFSGLYLIALGTGGIKPCV 180

Query: 181 SSFGADQFDDSDPAERVKKGSFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALF 240
           SSFGADQFD +DP+ERV+K SFFNWFYF+INIGA +SS++LVWIQ+N GW LGF IP +F
Sbjct: 181 SSFGADQFDKTDPSERVRKASFFNWFYFTINIGAFVSSTVLVWIQENYGWELGFLIPTVF 240

Query: 241 MGLAIISFFSGTKLYRFQKPGGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAI 300
           MGLA +SFF GT LYRFQKP GSPIT +CQVLVA++RK NLKVP DS       D+  A 
Sbjct: 241 MGLATMSFFFGTPLYRFQKPRGSPITSVCQVLVAAYRKSNLKVPEDST------DEGDA- 300

Query: 301 EGSRKLEHSNELRCLDKAAVISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGI 360
                                          ++PW+LCTVTQ+EE KIL+R+ PIWA+GI
Sbjct: 301 ------------------------------NTNPWKLCTVTQVEEVKILLRLVPIWASGI 360

Query: 361 VFAAVYAQMSTLFVEQGTMLDKTIGSFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKL 420
           +F+ +++Q+ TLFV+QG  + +TIG F IPPA+L  FD  SV+  VP+YDR IVP+ R+ 
Sbjct: 361 IFSVLHSQIYTLFVQQGRCMKRTIGLFEIPPATLGMFDTASVLISVPIYDRVIVPLVRRF 420

Query: 421 TGKERGFTEIQRMGIGLCISVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQ 480
           TG  +GFTE+QRMGIGL +SV  ++ AA+VE  RL LAR+LDLV   + VPL+I WQIPQ
Sbjct: 421 TGLAKGFTELQRMGIGLFVSVLSLTFAAIVETVRLQLARDLDLVESGDIVPLNIFWQIPQ 480

Query: 481 YFLLGAAEVFTFIGQLEFFYDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRN 540
           YFL+G A VF F+G++EFFY+QSPD+MRSLCSA +LLT  LGNYLSS I+T+V YL   +
Sbjct: 481 YFLMGTAGVFFFVGRIEFFYEQSPDSMRSLCSAWALLTTTLGNYLSSLIITLVAYL---S 540

Query: 541 GQAGWIP-DNLNEGHLDFFFWMLAGMSFLNLLVYIVCAKRYRPKK 583
           G+  WIP DN+N GHLD+FFW+L  +  +N+ V++  + +Y   K
Sbjct: 541 GKDCWIPSDNINNGHLDYFFWLLVSLGSVNIPVFVFFSVKYTHMK 544

BLAST of CmaCh04G021600 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 709.5 bits (1830), Expect = 2.2e-204
Identity = 345/567 (60.85%), Postives = 430/567 (75.84%), Query Frame = 0

Query: 19  ESNGLYTGDGSVDFHGNPVLKQNTGNWKACPFILGNEGCERLAYYGISTNLVTYLTNKLH 78
           +   +YT DG++D H  P  K  TG WKAC FILG E CERLAYYG+STNL+ YL  +++
Sbjct: 3   DDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMN 62

Query: 79  QGNVAAARNVTTWQGTCYLTPLIGAILADAYWGRYWTIAAFSTIYFIGMCTLTLSASVPA 138
             NV+A+++V+ W GTCY TPLIGA +ADAY GRYWTIA+F  IY  GM  LT+SASVP 
Sbjct: 63  MENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPG 122

Query: 139 LKPAECVGFMCPPASAAQYIVFFLGLYLIALGTGGIKPCVSSFGADQFDDSDPAERVKKG 198
           L P  C G  C  A+A Q  + F+ LYLIALGTGGIKPCVSSFGADQFDD+D  E+  K 
Sbjct: 123 LTPT-CSGETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKS 182

Query: 199 SFFNWFYFSINIGALISSSLLVWIQDNAGWGLGFGIPALFMGLAIISFFSGTKLYRFQKP 258
           SFFNWFYF IN+GA+I+SS+LVWIQ N GWG G G+P + M +A++ FF+G+  YR QKP
Sbjct: 183 SFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKP 242

Query: 259 GGSPITRMCQVLVASFRKRNLKVPGDSNLLYEVQDKSSAIEGSRKLEHSNELRCLDKAAV 318
           GGSP+TRM QV+VAS RK  +K+P D +LLYE QD  S+I GSRKLEH+  L   DKAAV
Sbjct: 243 GGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAV 302

Query: 319 ISDAELKSGDFSDPWRLCTVTQIEEFKILIRMFPIWATGIVFAAVYAQMSTLFVEQGTML 378
            ++++ K    S  W+LCTVTQ+EE K LIR+ PIWATGIVFA+VY+QM T+FV QG  L
Sbjct: 303 ETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTL 362

Query: 379 DKTIG-SFHIPPASLSTFDVVSVIFWVPVYDRFIVPIARKLTGKERGFTEIQRMGIGLCI 438
           D+ +G +F IP ASLS FD +SV+FW PVYD+ IVP ARK TG ERGFT++QR+GIGL I
Sbjct: 363 DQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVI 422

Query: 439 SVFCMSAAAVVEIKRLSLARELDLVHKPEAVPLSILWQIPQYFLLGAAEVFTFIGQLEFF 498
           S+F M +A ++E+ RL+  +  +L +  E +P++I WQ+PQYFL+G AEVFTFIGQLEFF
Sbjct: 423 SIFSMVSAGILEVARLNYVQTHNL-YNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFF 482

Query: 499 YDQSPDAMRSLCSALSLLTNALGNYLSSFILTIVTYLTTRNGQAGWIPDNLNEGHLDFFF 558
           YDQ+PDAMRSLCSALSL   A GNYLS+F++T+VT +T   G+ GWI  NLN GHLD+FF
Sbjct: 483 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFF 542

Query: 559 WMLAGMSFLNLLVYIVCAKRYRPKKAS 585
           W+LAG+SFLN LVY+  AK Y  KK +
Sbjct: 543 WLLAGLSFLNFLVYLWIAKWYTYKKTT 566

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P460327.0e-26475.43Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z201.1e-22162.73Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Q9M3902.9e-20962.26Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q84WG03.0e-20660.51Protein NRT1/ PTR FAMILY 8.4 OS=Arabidopsis thaliana OX=3702 GN=NPF8.4 PE=2 SV=2[more]
Q9LFB83.1e-20360.85Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JTA60.0e+00100.00protein NRT1/ PTR FAMILY 8.3-like OS=Cucurbita maxima OX=3661 GN=LOC111487649 PE... [more]
A0A6J1HCP40.0e+0099.83protein NRT1/ PTR FAMILY 8.3-like OS=Cucurbita moschata OX=3662 GN=LOC111462778 ... [more]
A0A5A7VM510.0e+0095.72Protein NRT1/ PTR FAMILY 8.3 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CTB90.0e+0095.55protein NRT1/ PTR FAMILY 8.3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103504109 ... [more]
A0A0A0KMV50.0e+0095.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G106020 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022990899.10.0e+00100.00protein NRT1/ PTR FAMILY 8.3-like [Cucurbita maxima][more]
XP_022962286.10.0e+0099.83protein NRT1/ PTR FAMILY 8.3-like [Cucurbita moschata] >XP_023543658.1 protein N... [more]
KAG7032606.10.0e+0099.82Protein NRT1/ PTR FAMILY 8.3, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_038875238.10.0e+0096.06protein NRT1/ PTR FAMILY 8.3 [Benincasa hispida][more]
KAA0067586.10.0e+0095.72protein NRT1/ PTR FAMILY 8.3 isoform X2 [Cucumis melo var. makuwa] >TYJ97173.1 p... [more]
Match NameE-valueIdentityDescription
AT2G02040.15.0e-26575.43peptide transporter 2 [more]
AT1G62200.18.0e-22362.73Major facilitator superfamily protein [more]
AT3G54140.12.0e-21062.26peptide transporter 1 [more]
AT2G02020.12.1e-20760.51Major facilitator superfamily protein [more]
AT5G01180.12.2e-20460.85peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 113..544
e-value: 3.3E-136
score: 454.3
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 32..581
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 17..584
e-value: 7.3E-220
score: 732.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 41..582
NoneNo IPR availablePANTHERPTHR11654:SF64PROTEIN NRT1/ PTR FAMILY 8.3coord: 32..581
NoneNo IPR availableCDDcd17418MFS_NPF8coord: 45..579
e-value: 0.0
score: 800.916
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01023PTR2_2coord: 201..213
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 102..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G021600.1CmaCh04G021600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity