CmaCh04G021340 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh04G021340 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 4.0e-193
Identity = 345/620 (55.65%), Postives = 437/620 (70.48%), Query Frame = 0

Query: 2   GDGIVDAALASLGKGFDLTSDFKLKYCK------GQHRLVLLNQAQRRDLIVPGFGAVKD 61
           G  +++ A+ SLGKGFDLT+DF+LKYCK      G  RLV+L+Q Q R+L +PGFG  ++
Sbjct: 5   GGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQN 64

Query: 62  ISIDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAE 121
           +S DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FNA FGFQSG WA DA 
Sbjct: 65  VSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAA 124

Query: 122 NTKCLGLDGYFISLFNAHI-DRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLS 181
           N K LGLD   ++LFN HI +   LRL+D VR AVP+SWDP  +ARFIE+YGTH+I G+S
Sbjct: 125 NVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVS 184

Query: 182 IGGHDVVLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHH---KTKLPQ 241
           +GG DVV+VRQDKSS+L+   L+ HL +LGDQLFTG+C           HH   + K P+
Sbjct: 185 VGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPE 244

Query: 242 AFNLFDPK-LVAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFI 301
           AFN+FD K  VA+ NF S+++++GI VI +K+GG   A +H EWL TV + PDAI+F FI
Sbjct: 245 AFNVFDDKQTVAFNNF-SINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 304

Query: 302 PIISLLDRIPGKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNIT 361
           PI SLL  +PG G LSHA++LYLRYKPP+ DLQYFLDF   + WAP+HNDLP G  PN+ 
Sbjct: 305 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 364

Query: 362 TS-PYLQPNLMGPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQN 421
           ++ P L  N MGPKLYVNTT VTSE  PVTG+R +LEG K NRLAIH+QHL  T   +  
Sbjct: 365 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 424

Query: 422 NITQHNPPLWHSSLHVAD-DRYVEPLNRNKFSYVSTAPVKYDPTWATATA---TPTPAAF 481
            IT  +  +W  S  + D DRY EPLN  KFS+V T PVKYDP W   T+   +    AF
Sbjct: 425 KITDEH--IWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAF 484

Query: 482 IVTGAQLHVVKHHDSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLS 541
           IVTGAQL  VK H SK+ LHL L Y+KVS+ YVVQ +W       S KSGIFS++S  L+
Sbjct: 485 IVTGAQLE-VKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLT 544

Query: 542 GSASAPKKKPPDPATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIG 601
             +        D   V++DSG+F  GPPVP    K+VKFV+LS++ +GPQ SPGHW+V G
Sbjct: 545 SGSVHHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTG 604

Query: 602 ARLNLENGKICLHVKFGLVH 606
            RL L+ GK+CLHVKF L+H
Sbjct: 605 VRLYLDKGKLCLHVKFALLH 619

BLAST of CmaCh04G021340 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 2.2e-135
Identity = 270/605 (44.63%), Postives = 374/605 (61.82%), Query Frame = 0

Query: 7   DAALASLGKGFDLTSDFKLKYCKG---QHRLVLLNQAQRR-DLIVPGFGAVKDISIDIKC 66
           + A+ S+G G+DL  D +LKYCKG     RL+ + +     ++++PG  ++ ++S  IKC
Sbjct: 12  EVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKC 71

Query: 67  DRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCLGL 126
           D+G+R R++SDIL F QM+E FN++ S+ GKIPSG FNAMF F S CW  DA  TK L  
Sbjct: 72  DKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYTKNLAF 131

Query: 127 DGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDVVL 186
           DG FISL++  +D+  + L + V+ AVP++WDP A+ARFI+ YGTHIIV + +GG DV+ 
Sbjct: 132 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 191

Query: 187 VRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHS---KPKDHHKTKLPQAFNLFDPK 246
            +Q  SS L+P +L+  L+E+ D+ F  A       S   +     +TK  +        
Sbjct: 192 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSS 251

Query: 247 LVAYANFSSVSAKDGIIVITSKKGGSPTAS-THCEWLPTVLESPDAIHFQFIPIISLLDR 306
           L +YAN      K+  + +  ++GG+   +  H EWL TV   PD I   FIPI SLL+ 
Sbjct: 252 LGSYAN------KEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNG 311

Query: 307 IPGKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPN 366
           +PG GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +   LQ +
Sbjct: 312 VPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFS 371

Query: 367 LMGPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPL 426
             GPKLYVNTT V    +P+TG+RLYLEG +SNRLAIH+QHL + P + Q     +    
Sbjct: 372 FFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSIR 431

Query: 427 WHSSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHH 486
             S     D RY E +N   +S+V T PV+ D   +           +VTGAQLH V+ H
Sbjct: 432 QESH----DRRYYEKVNWKNYSHVCTEPVESDDDLS-----------VVTGAQLH-VESH 491

Query: 487 DSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDP 546
             K  L L L +S+V  + +V+ +    +   +PKSG+   IST +S   +A +K PP P
Sbjct: 492 GFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGL---ISTLISHHFTAAQKPPPRP 551

Query: 547 ATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLH 604
           A V ++S I+  GPPVP QA KL+KFV+ SEM +GPQ+SPG+WVV GARL +E GKI L 
Sbjct: 552 ADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLK 590

BLAST of CmaCh04G021340 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 7.1e-126
Identity = 260/613 (42.41%), Postives = 366/613 (59.71%), Query Frame = 0

Query: 7   DAALASLGKGFDLTSDFKLKYCK---GQHRLVLLNQAQRRDLIVPGFGAVKDISIDIKCD 66
           + A++ +G G+DL SD +   CK      RLV ++  + RDLI PG   V ++S  IKCD
Sbjct: 16  EKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 75

Query: 67  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCLGLD 126
           +G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN MF F S CW  DA + K L  D
Sbjct: 76  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSVKTLAYD 135

Query: 127 GYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 186
           G+FISL++  I R  L L DEV+  VP+SWD  A+A FIEKYGTH++VG+++GG DV+ V
Sbjct: 136 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 195

Query: 187 RQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKLVAY 246
           +Q + SN EP E++  L+  GD+ F   C  P     P   +  K P+  NL    L  +
Sbjct: 196 KQMRKSNHEPEEIQKMLKHWGDERF---CVDPVESKSPASVYSGK-PKEENLLQWGLQPF 255

Query: 247 ANFSSVSA-----KDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDR 306
               S +       + I+ +  ++GG     +H  WL TV ++P+ I   F+PI SLL  
Sbjct: 256 GTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSG 315

Query: 307 IPGKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPN 366
           +PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +SP LQ +
Sbjct: 316 LPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFS 375

Query: 367 LMGPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPL 426
           LMGPKLYVNT++V S  +PVTGLR +LEG K N LAIH+QHL   P  L  +      P+
Sbjct: 376 LMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPI 435

Query: 427 WHSSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHH 486
                   +  Y  P+    FS+V T PV+Y+   +  TA+      IVT A L  VK  
Sbjct: 436 EEP----VEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTAS------IVTKAWLE-VKGM 495

Query: 487 DSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDP 546
             +  L L L +S  +++   ++ W   S++ S KSG+FS IST LS   S         
Sbjct: 496 GMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRKSGVFSMISTRLSTGLSPNPATTKPQ 555

Query: 547 ATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLH 606
           + + ++S ++  GP  P++  KL+  V+  E+ +GP++ PG+WVV GA+L +E GKI + 
Sbjct: 556 SKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIK 610

Query: 607 VKFGLVHVVSETS 612
            K+ L+ V+SE S
Sbjct: 616 AKYSLLTVISEDS 610

BLAST of CmaCh04G021340 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 5.1e-108
Identity = 240/605 (39.67%), Postives = 333/605 (55.04%), Query Frame = 0

Query: 9   ALASLGKGFDLTSDFKLKYCKGQ--HRLVLLNQAQRRDLIVPGFGAVKDISIDIKCDRGD 68
           A+ +LG+GFD+TSD +L YCKG    RLV + + Q RDL +     + ++  DI C RG+
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRGN 83

Query: 69  RTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCLGLDGYF 128
               +  + +F++M+E FN +  + G IP G FNAMF + +G W +DA +TK L L GYF
Sbjct: 84  SGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALVGYF 143

Query: 129 ISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLVRQD 188
           I L++  + +  L L +E+R AVP+SWDP ++A FIE YGTHI+  ++IGG DVV +RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 189 KSSNLEPSELKSHLEELGDQLFTGA---CTFPPPHSKPKDHHKTKLPQAFNLFDPKLVAY 248
           +SS L  SE+++++ ++    F  A       P   K KD                    
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKD-------------------- 263

Query: 249 ANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIPGKG 308
                      I VI  ++GG     +H  W  TV  +PD I+  F PI+SLL+ +PG  
Sbjct: 264 -----------ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLR 323

Query: 309 FLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLMGPK 368
            L+ AI LYL YKPPI DLQYFLD+Q+ + WAP  ++L             LQ +LMGPK
Sbjct: 324 HLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPK 383

Query: 369 LYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNP---PLWH 428
           L+++  QVT   KPVTGLRL LEG K NRL+IH+QHL + P +LQ +   H P   P W 
Sbjct: 384 LFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQ 443

Query: 429 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 488
                 D R+ EP+    FS+VST+P+++  T     +       IVTGAQL  V    S
Sbjct: 444 GP-EEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLS----GVHIVTGAQLG-VWDFGS 503

Query: 489 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 548
           K  LHL LL+SKV    + ++ W     D +P               AS+ + +P  P+T
Sbjct: 504 KNVLHLKLLFSKVPGCTIRRSVW-----DHTP--------------VASSGRLEPGGPST 560

Query: 549 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 606
                        V  Q+ KL K V+ SEM KGPQD PGHW+V GA+L +E GKI L VK
Sbjct: 564 -------SSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVK 560

BLAST of CmaCh04G021340 vs. ExPASy TrEMBL
Match: A0A6J1JPD7 (MACPF domain-containing protein At1g14780 OS=Cucurbita maxima OX=3661 GN=LOC111487690 PE=4 SV=1)

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 615/615 (100.00%), Postives = 615/615 (100.00%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL
Sbjct: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 480
           SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 480

Query: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 540
           KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT
Sbjct: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 540

Query: 541 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 600
           VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK
Sbjct: 541 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 600

Query: 601 FGLVHVVSETSGDEQ 616
           FGLVHVVSETSGDEQ
Sbjct: 601 FGLVHVVSETSGDEQ 615

BLAST of CmaCh04G021340 vs. ExPASy TrEMBL
Match: A0A6J1GM16 (MACPF domain-containing protein At1g14780 OS=Cucurbita moschata OX=3662 GN=LOC111455473 PE=4 SV=1)

HSP 1 Score: 1229.9 bits (3181), Expect = 0.0e+00
Identity = 595/615 (96.75%), Postives = 606/615 (98.54%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           MGDGIVDAALASLGKGFDLTSDFKLKYCKG+HRLVLLN+AQRRDLIVPGFG VKD+SIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRRDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FNAMFGFQSGCWAMDA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRAAVP+SWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSSNLE SELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDR+P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNITTSPYLQPNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 480
           SSLHVADDRYVEPLNRNKFSYVSTAPV YDPTW  ATATPTPAAFIVTGAQLHVVKHHDS
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTW--ATATPTPAAFIVTGAQLHVVKHHDS 480

Query: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 540
           KAALHLGLLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSLSG++SAPK+KPPDPAT
Sbjct: 481 KAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLSGASSAPKEKPPDPAT 540

Query: 541 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 600
           VIVDSG+FHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLEN KICLHVK
Sbjct: 541 VIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSKICLHVK 600

Query: 601 FGLVHVVSETSGDEQ 616
           FGLVHVVSETS DEQ
Sbjct: 601 FGLVHVVSETSADEQ 613

BLAST of CmaCh04G021340 vs. ExPASy TrEMBL
Match: A0A5D3CRV5 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00330 PE=4 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 525/616 (85.23%), Postives = 567/616 (92.05%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           M D IV  AL SLGKGFDLTSDF+LKYCKG H LVLLNQ+QRRDLIVPGFGAVKD+S+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWA DA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVP++WDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSS LEPSELK+HLEELGDQLF GACTFPPP SKPKDHHK K+PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VA+ NFSSVSAKDGIIVITSK+GGSPTAS+HCEWLPTV ESPDAIHFQFIPIISLL+ IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNI+TSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQV ++NKPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ     WH
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATP-TPAAFIVTGAQLHVVKHHD 480
           SS H+ADDRYVEPLNRNKFS+V T P+KYDP WAT +    +PAAFIVTGAQL VVK HD
Sbjct: 421 SSQHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHD 480

Query: 481 SKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPA 540
           SK  LHL LLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSL+G+++A K+KPP+ A
Sbjct: 481 SKTVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPE-A 540

Query: 541 TVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHV 600
            VIVDSG+FH GPP+ +Q QKLVKFVELSEMRKGPQDSPG+W+VIGARLNLE GKICL V
Sbjct: 541 AVIVDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQV 600

Query: 601 KFGLVHVVSETSGDEQ 616
           KFGL+HV+SET  D+Q
Sbjct: 601 KFGLLHVLSETITDDQ 613

BLAST of CmaCh04G021340 vs. ExPASy TrEMBL
Match: E5GB62 (MACPF domain-containing protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 525/616 (85.23%), Postives = 567/616 (92.05%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           M D IV  AL SLGKGFDLTSDF+LKYCKG H LVLLNQ+QRRDLIVPGFGAVKD+S+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWA DA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVP++WDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSS LEPSELK+HLEELGDQLF GACTFPPP SKPKDHHK K+PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VA+ NFSSVSAKDGIIVITSK+GGSPTAS+HCEWLPTV ESPDAIHFQFIPIISLL+ IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNI+TSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQV ++NKPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ     WH
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATP-TPAAFIVTGAQLHVVKHHD 480
           SS H+ADDRYVEPLNRNKFS+V T P+KYDP WAT +    +PAAFIVTGAQL VVK HD
Sbjct: 421 SSQHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHD 480

Query: 481 SKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPA 540
           SK  LHL LLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSL+G+++A K+KPP+ A
Sbjct: 481 SKTVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPE-A 540

Query: 541 TVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHV 600
            VIVDSG+FH GPP+ +Q QKLVKFVELSEMRKGPQDSPG+W+VIGARLNLE GKICL V
Sbjct: 541 AVIVDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQV 600

Query: 601 KFGLVHVVSETSGDEQ 616
           KFGL+HV+SET  D+Q
Sbjct: 601 KFGLLHVLSETITDDQ 613

BLAST of CmaCh04G021340 vs. ExPASy TrEMBL
Match: A0A1S3CSS8 (LOW QUALITY PROTEIN: MACPF domain-containing protein At1g14780-like OS=Cucumis melo OX=3656 GN=LOC103504476 PE=4 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 525/616 (85.23%), Postives = 567/616 (92.05%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           M D IV  AL SLGKGFDLTSDF+LKYCKG H LVLLNQ+QRRDLIVPGFGAVKD+S+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWA DA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVP++WDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSS LEPSELK+HLEELGDQLF GACTFPPP SKPKDHHK K+PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VA+ NFSSVSAKDGIIVITSK+GGSPTAS+HCEWLPTV ESPDAIHFQFIPIISLL+ IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNI+TSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQV ++NKPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ     WH
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATP-TPAAFIVTGAQLHVVKHHD 480
           SS H+ADDRYVEPLNRNKFS+V T P+KYDP WAT +    +PAAFIVTGAQL VVK HD
Sbjct: 421 SSQHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHD 480

Query: 481 SKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPA 540
           SK  LHL LLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSL+G+++A K+KPP+ A
Sbjct: 481 SKXVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPE-A 540

Query: 541 TVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHV 600
            VIVDSG+FH GPP+ +Q QKLVKFVELSEMRKGPQDSPG+W+VIGARLNLE GKICL V
Sbjct: 541 AVIVDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQV 600

Query: 601 KFGLVHVVSETSGDEQ 616
           KFGL+HV+SET  D+Q
Sbjct: 601 KFGLLHVLSETITDDQ 613

BLAST of CmaCh04G021340 vs. NCBI nr
Match: XP_022990956.1 (MACPF domain-containing protein At1g14780 [Cucurbita maxima])

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 615/615 (100.00%), Postives = 615/615 (100.00%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL
Sbjct: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 480
           SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 480

Query: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 540
           KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT
Sbjct: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 540

Query: 541 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 600
           VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK
Sbjct: 541 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 600

Query: 601 FGLVHVVSETSGDEQ 616
           FGLVHVVSETSGDEQ
Sbjct: 601 FGLVHVVSETSGDEQ 615

BLAST of CmaCh04G021340 vs. NCBI nr
Match: XP_022952953.1 (MACPF domain-containing protein At1g14780 [Cucurbita moschata] >XP_022952961.1 MACPF domain-containing protein At1g14780 [Cucurbita moschata])

HSP 1 Score: 1229.9 bits (3181), Expect = 0.0e+00
Identity = 595/615 (96.75%), Postives = 606/615 (98.54%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           MGDGIVDAALASLGKGFDLTSDFKLKYCKG+HRLVLLN+AQRRDLIVPGFG VKD+SIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRRDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FNAMFGFQSGCWAMDA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRAAVP+SWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSSNLE SELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDR+P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNITTSPYLQPNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 480
           SSLHVADDRYVEPLNRNKFSYVSTAPV YDPTW  ATATPTPAAFIVTGAQLHVVKHHDS
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTW--ATATPTPAAFIVTGAQLHVVKHHDS 480

Query: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 540
           KAALHLGLLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSLSG++SAPK+KPPDPAT
Sbjct: 481 KAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLSGASSAPKEKPPDPAT 540

Query: 541 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 600
           VIVDSG+FHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLEN KICLHVK
Sbjct: 541 VIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSKICLHVK 600

Query: 601 FGLVHVVSETSGDEQ 616
           FGLVHVVSETS DEQ
Sbjct: 601 FGLVHVVSETSADEQ 613

BLAST of CmaCh04G021340 vs. NCBI nr
Match: XP_023546039.1 (MACPF domain-containing protein At1g14780 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 595/615 (96.75%), Postives = 605/615 (98.37%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLN+AQRRDLIVPGFGA+KD+SIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNEAQRRDLIVPGFGAIKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FNAMFGFQSGCWAMDA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRAAVP+SWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGQDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSSNL+PSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFD KL
Sbjct: 181 VLVRQDKSSNLQPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDTKL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDR+P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNITTSPYLQPNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQVTSE KPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH
Sbjct: 361 GPKLYVNTTQVTSEKKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 480
           SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTW  ATATPTPAAFIVTGAQLHVVKHHDS
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTW--ATATPTPAAFIVTGAQLHVVKHHDS 480

Query: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 540
           KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSG+ SAPK+KPPDPAT
Sbjct: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGATSAPKEKPPDPAT 540

Query: 541 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 600
           VIVDSG+FHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLEN KICLHVK
Sbjct: 541 VIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSKICLHVK 600

Query: 601 FGLVHVVSETSGDEQ 616
           FGLVHVVSETS DEQ
Sbjct: 601 FGLVHVVSETSADEQ 613

BLAST of CmaCh04G021340 vs. NCBI nr
Match: KAG7032583.1 (MACPF domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1228.4 bits (3177), Expect = 0.0e+00
Identity = 594/615 (96.59%), Postives = 606/615 (98.54%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           MGDGIVDAALASLGKGFDLTSDFKLKYCKG+HRLVLLN+AQRRDLIVPGFG VKD+SIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRRDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FNA+FGFQSGCWAMDA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAIFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRAAVP+SWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSSNLE SELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDR+P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNITTSPYLQPNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 480
           SSLHVADDRYVEPLNRNKFSYVSTAPV YDPTW  ATATPTPAAFIVTGAQLHVVKHHDS
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTW--ATATPTPAAFIVTGAQLHVVKHHDS 480

Query: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 540
           KAALHLGLLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSLSG++SAPK+KPPDPAT
Sbjct: 481 KAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLSGASSAPKEKPPDPAT 540

Query: 541 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 600
           VIVDSG+FHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLEN KICLHVK
Sbjct: 541 VIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSKICLHVK 600

Query: 601 FGLVHVVSETSGDEQ 616
           FGLVHVVSETS DEQ
Sbjct: 601 FGLVHVVSETSADEQ 613

BLAST of CmaCh04G021340 vs. NCBI nr
Match: KAG6601885.1 (MACPF domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 593/615 (96.42%), Postives = 605/615 (98.37%), Query Frame = 0

Query: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60
           MGDGIVDAALASLGKGFDLTSDFKLKYCKG+HRLVLLN+AQR DLIVPGFG VKD+SIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRPDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FNAMFGFQ+GCWAMDA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQTGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRAAVP+SWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240
           VLVRQDKSSNLE SELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300
           VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDR+P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNITTSPYLQPNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420
           GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 480
           SSLHVADDRYVEPLNRNKFSYVSTAPV YDPTW  ATATPTPAAFIVTGAQLHVVKHHDS
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTW--ATATPTPAAFIVTGAQLHVVKHHDS 480

Query: 481 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 540
           KAALHLGLLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSLSG++SAPK+KPPDPAT
Sbjct: 481 KAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLSGASSAPKEKPPDPAT 540

Query: 541 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 600
           VIVDSG+FHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLEN KICLHVK
Sbjct: 541 VIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSKICLHVK 600

Query: 601 FGLVHVVSETSGDEQ 616
           FGLVHVVSETS DEQ
Sbjct: 601 FGLVHVVSETSADEQ 613

BLAST of CmaCh04G021340 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 676.0 bits (1743), Expect = 2.8e-194
Identity = 345/620 (55.65%), Postives = 437/620 (70.48%), Query Frame = 0

Query: 2   GDGIVDAALASLGKGFDLTSDFKLKYCK------GQHRLVLLNQAQRRDLIVPGFGAVKD 61
           G  +++ A+ SLGKGFDLT+DF+LKYCK      G  RLV+L+Q Q R+L +PGFG  ++
Sbjct: 5   GGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQN 64

Query: 62  ISIDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAE 121
           +S DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FNA FGFQSG WA DA 
Sbjct: 65  VSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAA 124

Query: 122 NTKCLGLDGYFISLFNAHI-DRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLS 181
           N K LGLD   ++LFN HI +   LRL+D VR AVP+SWDP  +ARFIE+YGTH+I G+S
Sbjct: 125 NVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVS 184

Query: 182 IGGHDVVLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHH---KTKLPQ 241
           +GG DVV+VRQDKSS+L+   L+ HL +LGDQLFTG+C           HH   + K P+
Sbjct: 185 VGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPE 244

Query: 242 AFNLFDPK-LVAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFI 301
           AFN+FD K  VA+ NF S+++++GI VI +K+GG   A +H EWL TV + PDAI+F FI
Sbjct: 245 AFNVFDDKQTVAFNNF-SINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 304

Query: 302 PIISLLDRIPGKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNIT 361
           PI SLL  +PG G LSHA++LYLRYKPP+ DLQYFLDF   + WAP+HNDLP G  PN+ 
Sbjct: 305 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 364

Query: 362 TS-PYLQPNLMGPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQN 421
           ++ P L  N MGPKLYVNTT VTSE  PVTG+R +LEG K NRLAIH+QHL  T   +  
Sbjct: 365 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 424

Query: 422 NITQHNPPLWHSSLHVAD-DRYVEPLNRNKFSYVSTAPVKYDPTWATATA---TPTPAAF 481
            IT  +  +W  S  + D DRY EPLN  KFS+V T PVKYDP W   T+   +    AF
Sbjct: 425 KITDEH--IWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAF 484

Query: 482 IVTGAQLHVVKHHDSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLS 541
           IVTGAQL  VK H SK+ LHL L Y+KVS+ YVVQ +W       S KSGIFS++S  L+
Sbjct: 485 IVTGAQLE-VKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLT 544

Query: 542 GSASAPKKKPPDPATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIG 601
             +        D   V++DSG+F  GPPVP    K+VKFV+LS++ +GPQ SPGHW+V G
Sbjct: 545 SGSVHHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTG 604

Query: 602 ARLNLENGKICLHVKFGLVH 606
            RL L+ GK+CLHVKF L+H
Sbjct: 605 VRLYLDKGKLCLHVKFALLH 619

BLAST of CmaCh04G021340 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 484.2 bits (1245), Expect = 1.6e-136
Identity = 270/605 (44.63%), Postives = 374/605 (61.82%), Query Frame = 0

Query: 7   DAALASLGKGFDLTSDFKLKYCKG---QHRLVLLNQAQRR-DLIVPGFGAVKDISIDIKC 66
           + A+ S+G G+DL  D +LKYCKG     RL+ + +     ++++PG  ++ ++S  IKC
Sbjct: 12  EVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKC 71

Query: 67  DRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCLGL 126
           D+G+R R++SDIL F QM+E FN++ S+ GKIPSG FNAMF F S CW  DA  TK L  
Sbjct: 72  DKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYTKNLAF 131

Query: 127 DGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDVVL 186
           DG FISL++  +D+  + L + V+ AVP++WDP A+ARFI+ YGTHIIV + +GG DV+ 
Sbjct: 132 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 191

Query: 187 VRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHS---KPKDHHKTKLPQAFNLFDPK 246
            +Q  SS L+P +L+  L+E+ D+ F  A       S   +     +TK  +        
Sbjct: 192 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSS 251

Query: 247 LVAYANFSSVSAKDGIIVITSKKGGSPTAS-THCEWLPTVLESPDAIHFQFIPIISLLDR 306
           L +YAN      K+  + +  ++GG+   +  H EWL TV   PD I   FIPI SLL+ 
Sbjct: 252 LGSYAN------KEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNG 311

Query: 307 IPGKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPN 366
           +PG GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +   LQ +
Sbjct: 312 VPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFS 371

Query: 367 LMGPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPL 426
             GPKLYVNTT V    +P+TG+RLYLEG +SNRLAIH+QHL + P + Q     +    
Sbjct: 372 FFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSIR 431

Query: 427 WHSSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHH 486
             S     D RY E +N   +S+V T PV+ D   +           +VTGAQLH V+ H
Sbjct: 432 QESH----DRRYYEKVNWKNYSHVCTEPVESDDDLS-----------VVTGAQLH-VESH 491

Query: 487 DSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDP 546
             K  L L L +S+V  + +V+ +    +   +PKSG+   IST +S   +A +K PP P
Sbjct: 492 GFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGL---ISTLISHHFTAAQKPPPRP 551

Query: 547 ATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLH 604
           A V ++S I+  GPPVP QA KL+KFV+ SEM +GPQ+SPG+WVV GARL +E GKI L 
Sbjct: 552 ADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLK 590

BLAST of CmaCh04G021340 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 452.6 bits (1163), Expect = 5.1e-127
Identity = 260/613 (42.41%), Postives = 366/613 (59.71%), Query Frame = 0

Query: 7   DAALASLGKGFDLTSDFKLKYCK---GQHRLVLLNQAQRRDLIVPGFGAVKDISIDIKCD 66
           + A++ +G G+DL SD +   CK      RLV ++  + RDLI PG   V ++S  IKCD
Sbjct: 16  EKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 75

Query: 67  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCLGLD 126
           +G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN MF F S CW  DA + K L  D
Sbjct: 76  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSVKTLAYD 135

Query: 127 GYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 186
           G+FISL++  I R  L L DEV+  VP+SWD  A+A FIEKYGTH++VG+++GG DV+ V
Sbjct: 136 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 195

Query: 187 RQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKLVAY 246
           +Q + SN EP E++  L+  GD+ F   C  P     P   +  K P+  NL    L  +
Sbjct: 196 KQMRKSNHEPEEIQKMLKHWGDERF---CVDPVESKSPASVYSGK-PKEENLLQWGLQPF 255

Query: 247 ANFSSVSA-----KDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDR 306
               S +       + I+ +  ++GG     +H  WL TV ++P+ I   F+PI SLL  
Sbjct: 256 GTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSG 315

Query: 307 IPGKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPN 366
           +PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +SP LQ +
Sbjct: 316 LPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFS 375

Query: 367 LMGPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPL 426
           LMGPKLYVNT++V S  +PVTGLR +LEG K N LAIH+QHL   P  L  +      P+
Sbjct: 376 LMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPI 435

Query: 427 WHSSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHH 486
                   +  Y  P+    FS+V T PV+Y+   +  TA+      IVT A L  VK  
Sbjct: 436 EEP----VEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTAS------IVTKAWLE-VKGM 495

Query: 487 DSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDP 546
             +  L L L +S  +++   ++ W   S++ S KSG+FS IST LS   S         
Sbjct: 496 GMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRKSGVFSMISTRLSTGLSPNPATTKPQ 555

Query: 547 ATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLH 606
           + + ++S ++  GP  P++  KL+  V+  E+ +GP++ PG+WVV GA+L +E GKI + 
Sbjct: 556 SKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIK 610

Query: 607 VKFGLVHVVSETS 612
            K+ L+ V+SE S
Sbjct: 616 AKYSLLTVISEDS 610

BLAST of CmaCh04G021340 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 393.3 bits (1009), Expect = 3.6e-109
Identity = 240/605 (39.67%), Postives = 333/605 (55.04%), Query Frame = 0

Query: 9   ALASLGKGFDLTSDFKLKYCKGQ--HRLVLLNQAQRRDLIVPGFGAVKDISIDIKCDRGD 68
           A+ +LG+GFD+TSD +L YCKG    RLV + + Q RDL +     + ++  DI C RG+
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRGN 83

Query: 69  RTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCLGLDGYF 128
               +  + +F++M+E FN +  + G IP G FNAMF + +G W +DA +TK L L GYF
Sbjct: 84  SGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALVGYF 143

Query: 129 ISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLVRQD 188
           I L++  + +  L L +E+R AVP+SWDP ++A FIE YGTHI+  ++IGG DVV +RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 189 KSSNLEPSELKSHLEELGDQLFTGA---CTFPPPHSKPKDHHKTKLPQAFNLFDPKLVAY 248
           +SS L  SE+++++ ++    F  A       P   K KD                    
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKD-------------------- 263

Query: 249 ANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIPGKG 308
                      I VI  ++GG     +H  W  TV  +PD I+  F PI+SLL+ +PG  
Sbjct: 264 -----------ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLR 323

Query: 309 FLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLMGPK 368
            L+ AI LYL YKPPI DLQYFLD+Q+ + WAP  ++L             LQ +LMGPK
Sbjct: 324 HLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPK 383

Query: 369 LYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNP---PLWH 428
           L+++  QVT   KPVTGLRL LEG K NRL+IH+QHL + P +LQ +   H P   P W 
Sbjct: 384 LFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQ 443

Query: 429 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATPTPAAFIVTGAQLHVVKHHDS 488
                 D R+ EP+    FS+VST+P+++  T     +       IVTGAQL  V    S
Sbjct: 444 GP-EEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLS----GVHIVTGAQLG-VWDFGS 503

Query: 489 KAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGSASAPKKKPPDPAT 548
           K  LHL LL+SKV    + ++ W     D +P               AS+ + +P  P+T
Sbjct: 504 KNVLHLKLLFSKVPGCTIRRSVW-----DHTP--------------VASSGRLEPGGPST 560

Query: 549 VIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGKICLHVK 606
                        V  Q+ KL K V+ SEM KGPQD PGHW+V GA+L +E GKI L VK
Sbjct: 564 -------SSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVK 560

BLAST of CmaCh04G021340 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 264.6 bits (675), Expect = 2.0e-70
Identity = 140/319 (43.89%), Postives = 198/319 (62.07%), Query Frame = 0

Query: 7   DAALASLGKGFDLTSDFKLKYCKG---QHRLVLLNQAQRR-DLIVPGFGAVKDISIDIKC 66
           + A+ S+G G+DL  D +LKYCKG     RL+ + +     ++++PG  ++ ++S  IKC
Sbjct: 12  EVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKC 71

Query: 67  DRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCLGL 126
           D+G+R R++SDIL F QM+E FN++ S+ GKIPSG FNAMF F S CW  DA  TK L  
Sbjct: 72  DKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYTKNLAF 131

Query: 127 DGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDVVL 186
           DG FISL++  +D+  + L + V+ AVP++WDP A+ARFI+ YGTHIIV + +GG DV+ 
Sbjct: 132 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 191

Query: 187 VRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHS---KPKDHHKTKLPQAFNLFDPK 246
            +Q  SS L+P +L+  L+E+ D+ F  A       S   +     +TK  +        
Sbjct: 192 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSS 251

Query: 247 LVAYANFSSVSAKDGIIVITSKKGGSPTAS-THCEWLPTVLESPDAIHFQFIPIISLLDR 306
           L +YAN      K+  + +  ++GG+   +  H EWL TV   PD I   FIPI SLL+ 
Sbjct: 252 LGSYAN------KEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNG 311

Query: 307 IPGKGFLSHAINLYLRYKP 318
           +PG GFLSHAINLYLR KP
Sbjct: 312 VPGSGFLSHAINLYLRCKP 323