CmaCh04G021160 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G021160
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Cma_Chr04: 14775151 .. 14775789 (+)
RNA-Seq Expression	CmaCh04G021160
Synteny	CmaCh04G021160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACCTCAATCGCCTCTTATCCTCTCTCTCCTATTCGCAGCCACTCTCTGTCTCCTACGCCAGGTTCCGGCCGCCGCCGCCGCCGCTGCTGATGCTGGGACCTCCGTCAACGCCACTGCCAATGGTACCGAGTTTATCCGGAGTAGCTGTGTCATTACTCTCTATCCCGACGTCTGCTACGCTTCGCTCTCTCGCTACGCAAACGACATTCAGCAGGATCCAGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACTCCCGTAGAATGGCTTCTTATGTATCCAACCTGTCTCACGTCGGCGACTACGGCGCCGATCACAGAGCAGCCTCCGCGCTTCACGATTGCTTTACGAACTTCGACGACGCCGTCGACGAGATTCGCGGCTCGCTCAACCAGATGCGCCAGATCAACGACGTCGACGTGCCGTCGTTCAGGTTTCAAATGAGCAACGTGCAGACGTGGATGAGCGCCGCGCTTACGGATCAGGAAACATGTACGGACGGATTCGAAGATGTGGCTGATGGACCGATGAAAGCGGACGTATGCGCAAAGGCGGAGAAGGTGAAGAAGCACACCAGTAATGCCCTAGCGCTGGTGAATAGCTTCGTCCAGAAAAGGATTCCGTGA

mRNA sequence

ATGAAACCTCAATCGCCTCTTATCCTCTCTCTCCTATTCGCAGCCACTCTCTGTCTCCTACGCCAGGTTCCGGCCGCCGCCGCCGCCGCTGCTGATGCTGGGACCTCCGTCAACGCCACTGCCAATGGTACCGAGTTTATCCGGAGTAGCTGTGTCATTACTCTCTATCCCGACGTCTGCTACGCTTCGCTCTCTCGCTACGCAAACGACATTCAGCAGGATCCAGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACTCCCGTAGAATGGCTTCTTATGTATCCAACCTGTCTCACGTCGGCGACTACGGCGCCGATCACAGAGCAGCCTCCGCGCTTCACGATTGCTTTACGAACTTCGACGACGCCGTCGACGAGATTCGCGGCTCGCTCAACCAGATGCGCCAGATCAACGACGTCGACGTGCCGTCGTTCAGGTTTCAAATGAGCAACGTGCAGACGTGGATGAGCGCCGCGCTTACGGATCAGGAAACATGTACGGACGGATTCGAAGATGTGGCTGATGGACCGATGAAAGCGGACGTATGCGCAAAGGCGGAGAAGGTGAAGAAGCACACCAGTAATGCCCTAGCGCTGGTGAATAGCTTCGTCCAGAAAAGGATTCCGTGA

Coding sequence (CDS)

ATGAAACCTCAATCGCCTCTTATCCTCTCTCTCCTATTCGCAGCCACTCTCTGTCTCCTACGCCAGGTTCCGGCCGCCGCCGCCGCCGCTGCTGATGCTGGGACCTCCGTCAACGCCACTGCCAATGGTACCGAGTTTATCCGGAGTAGCTGTGTCATTACTCTCTATCCCGACGTCTGCTACGCTTCGCTCTCTCGCTACGCAAACGACATTCAGCAGGATCCAGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACTCCCGTAGAATGGCTTCTTATGTATCCAACCTGTCTCACGTCGGCGACTACGGCGCCGATCACAGAGCAGCCTCCGCGCTTCACGATTGCTTTACGAACTTCGACGACGCCGTCGACGAGATTCGCGGCTCGCTCAACCAGATGCGCCAGATCAACGACGTCGACGTGCCGTCGTTCAGGTTTCAAATGAGCAACGTGCAGACGTGGATGAGCGCCGCGCTTACGGATCAGGAAACATGTACGGACGGATTCGAAGATGTGGCTGATGGACCGATGAAAGCGGACGTATGCGCAAAGGCGGAGAAGGTGAAGAAGCACACCAGTAATGCCCTAGCGCTGGTGAATAGCTTCGTCCAGAAAAGGATTCCGTGA

Protein sequence

MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNALALVNSFVQKRIP

Homology

[Close]

BLAST of CmaCh04G021160 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.4e-28
Identity = 79/201 (39.30%), Postives = 117/201 (58.21%), Query Frame = 0


Query: 10  SLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVCYASLSRYAN 69
           +L+F+A L +L     AAA+AA A           +FI++SC +T YP VC  SLS YA 
Sbjct: 7   TLVFSALLVIL-----AAASAAPA----------NQFIKTSCTLTTYPAVCEQSLSAYAK 66

Query: 70  DIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDE 129
            IQ +P  L   A+ +SL  +++  +++  L+     G   R  +A+HDC    +D++D 
Sbjct: 67  TIQNNPQELASTALQVSLTRTQQAQTFMKRLNKF--KGLKARQYAAIHDCLEEVEDSLDR 126

Query: 130 IRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVA-DGPMKADVCAK 189
           +  S ++M+ ++      F F+MSNV+TW+SAALTD+ TC DGF     DG +K  V A+
Sbjct: 127 VSRSCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQ 186

Query: 190 AEKVKKHTSNALALVNSFVQK 210
              V + TSNALALVN+F  K
Sbjct: 187 VVAVARVTSNALALVNNFAAK 190

[Close]

BLAST of CmaCh04G021160 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.4e-25
Identity = 72/187 (38.50%), Postives = 105/187 (56.15%), Query Frame = 0


Query: 26  AAAAAADAGTSVNATANGTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITI 85
           + AA   A      ++  TEFI++SC  T YPD C+ SLS YA++I++ P  L   A+ +
Sbjct: 17  STAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAV 76

Query: 86  SLANSRRMASYVSNLSHVGDY-GADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDVD 145
           S+A ++   +YVS ++   DY G   R   A+ DC     D VD +  SL +++ + + D
Sbjct: 77  SIARAKSAKTYVSEMT---DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGD 136

Query: 146 V-PSFRFQMSNVQTWMSAALTDQETCTDGFEDVA-DGPMKADVCAKAEKVKKHTSNALAL 205
               F F +SNV+TW SAALTD+  C DGF   A  G +K+ +      V + TSNALAL
Sbjct: 137 SGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALAL 196

Query: 206 VNSFVQK 210
           +N F  K
Sbjct: 197 INDFASK 200

[Close]

BLAST of CmaCh04G021160 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-22
Identity = 69/206 (33.50%), Postives = 114/206 (55.34%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           + P SP  LSL  ++           ++ +  + +S  +     ++I++SC ITLY  +C
Sbjct: 102 LSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTIC 161

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           Y SLS YA+ I+ +P  L  +A+ ++L++++  + +V N+SH G  G       A+ DC 
Sbjct: 162 YNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGG--GLTRLEVVAVADCV 221

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
               D+V  ++ S+ ++  IN  D   F   MS+V+TW+SAALT+ +TC DGF  V    
Sbjct: 222 EEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGFSLVKTA- 281

Query: 181 MKADVCAKAEKVKKHTSNALALVNSF 207
           +K  V     +V + TSNALAL+N +
Sbjct: 282 VKDLVRRHVVEVARLTSNALALINMY 304

[Close]

BLAST of CmaCh04G021160 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 7.4e-22
Identity = 70/196 (35.71%), Postives = 100/196 (51.02%), Query Frame = 0


Query: 16  TLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVCYASLSRYANDIQQDP 75
           TL L     A   A++ A  +   +     FI++SC  T YP VC  SL+ YAN IQ  P
Sbjct: 8   TLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSP 67

Query: 76  ASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLN 135
             L   A+ +++  ++    +V  L          R   A+ DC     DAVD +  S++
Sbjct: 68  RRLAETALNVTVTQAQSTKVFVWRLGRF--TSLKKREIQAVKDCIEEIHDAVDRLTMSIH 127

Query: 136 QMRQINDV-DVPSFRFQMSNVQTWMSAALTDQETCTDGFED-VADGPMKADVCAKAEKVK 195
           +++          F F MSN QTW SAALT+  TC+DGF   V DG +K  V A+   + 
Sbjct: 128 EVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLG 187

Query: 196 KHTSNALALVNSFVQK 210
           + TSNALAL+N+F +K
Sbjct: 188 RGTSNALALINAFAKK 201

[Close]

BLAST of CmaCh04G021160 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.4e-20
Identity = 70/190 (36.84%), Postives = 103/190 (54.21%), Query Frame = 0


Query: 25  AAAAAAADAGTSVNATANGTE-FIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAI 84
           A A A     ++V+   NG E  + SSC    YP +C  +LS Y+     +   L + AI
Sbjct: 11  AIAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAI 70

Query: 85  TISLANSRRMASYVSNLSHVGD-YGADHRAASALHDCFTNFDDAVDEIRGSLNQMR--QI 144
            ISL++++   S    L+ V D  G   +  +AL DC     D+VDE+  +L  ++  ++
Sbjct: 71  KISLSHAQ---SAAKKLAVVRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRV 130

Query: 145 NDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNAL 204
           +      FR+QMSN QTW SAALTD +TC DGF+ + DG +K +V     KV + TSNAL
Sbjct: 131 SGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNAL 190

Query: 205 ALVNSFVQKR 211
            +VN   + R
Sbjct: 191 YMVNQLDETR 197

[Close]

BLAST of CmaCh04G021160 vs. ExPASy TrEMBL
Match: A0A6J1JSR9 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111487516 PE=4 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.2e-110
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC
Sbjct: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF
Sbjct: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           MKADVCAKAEKVKKHTSNALALVNSFVQKRIP
Sbjct: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 212

[Close]

BLAST of CmaCh04G021160 vs. ExPASy TrEMBL
Match: A0A6J1HFF2 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111463076 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.0e-106
Identity = 207/212 (97.64%), Postives = 208/212 (98.11%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           MKPQSPLILSLLF ATLCLLRQVP AAA AAD+GTSVNATAN TEFIRSSCVITLYPDVC
Sbjct: 1   MKPQSPLILSLLFVATLCLLRQVP-AAADAADSGTSVNATANSTEFIRSSCVITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF
Sbjct: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           MKADVCAKAEKVKKHTSNALALVNSFVQKRIP
Sbjct: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 211

[Close]

BLAST of CmaCh04G021160 vs. ExPASy TrEMBL
Match: A0A5A7TTZ6 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00440 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.2e-92
Identity = 184/212 (86.79%), Postives = 188/212 (88.68%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           MKPQ PLILSLLFAATL  LR V     A  D   S NATAN  EFIR+SC ITLYPDVC
Sbjct: 1   MKPQRPLILSLLFAATLFHLRPV----FANEDTRNSSNATANCIEFIRTSCGITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           Y SLSRYANDIQQDPASLTR+AITISLANSRRMA+YVSNLSH GDYGAD RAASALHDCF
Sbjct: 61  YTSLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHAGDYGADRRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           MK DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 MKEDVCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of CmaCh04G021160 vs. ExPASy TrEMBL
Match: A0A1S3CSS6 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103504548 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.2e-92
Identity = 184/212 (86.79%), Postives = 188/212 (88.68%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           MKPQ PLILSLLFAATL  LR V     A  D   S NATAN  EFIR+SC ITLYPDVC
Sbjct: 1   MKPQRPLILSLLFAATLFHLRPV----FANEDTRNSSNATANCIEFIRTSCGITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           Y SLSRYANDIQQDPASLTR+AITISLANSRRMA+YVSNLSH GDYGAD RAASALHDCF
Sbjct: 61  YTSLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHAGDYGADRRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           MK DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 MKEDVCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of CmaCh04G021160 vs. ExPASy TrEMBL
Match: A0A6J1FD40 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111444672 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.6e-91
Identity = 181/212 (85.38%), Postives = 192/212 (90.57%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           MKPQ   I+SLLFAA L LLR VP    +AADAGT  NATA+ +EFIR+SCVITLYPDVC
Sbjct: 1   MKPQPSPIVSLLFAAGLFLLRLVP----SAADAGTFPNATADCSEFIRTSCVITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           Y SLSRYAN IQQDPASLTRVAITISLAN+RRMA+YVSNLSHVGDYGADHR +SALHDCF
Sbjct: 61  YTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP
Sbjct: 121 TNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           +K DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 IKKDVCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of CmaCh04G021160 vs. NCBI nr
Match: XP_022990704.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 409.1 bits (1050), Expect = 2.4e-110
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC
Sbjct: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF
Sbjct: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           MKADVCAKAEKVKKHTSNALALVNSFVQKRIP
Sbjct: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 212

[Close]

BLAST of CmaCh04G021160 vs. NCBI nr
Match: XP_022962658.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 396.0 bits (1016), Expect = 2.1e-106
Identity = 207/212 (97.64%), Postives = 208/212 (98.11%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           MKPQSPLILSLLF ATLCLLRQVP AAA AAD+GTSVNATAN TEFIRSSCVITLYPDVC
Sbjct: 1   MKPQSPLILSLLFVATLCLLRQVP-AAADAADSGTSVNATANSTEFIRSSCVITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF
Sbjct: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           MKADVCAKAEKVKKHTSNALALVNSFVQKRIP
Sbjct: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 211

[Close]

BLAST of CmaCh04G021160 vs. NCBI nr
Match: KAG6601869.1 (hypothetical protein SDJN03_07102, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032570.1 hypothetical protein SDJN02_06619, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 396.0 bits (1016), Expect = 2.1e-106
Identity = 207/212 (97.64%), Postives = 208/212 (98.11%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           M+PQSPLILSLLF ATLCLLRQVP AAA AADAGTSVNATAN TEFIRSSCVITLYPDVC
Sbjct: 1   MRPQSPLILSLLFVATLCLLRQVP-AAADAADAGTSVNATANSTEFIRSSCVITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF
Sbjct: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           MKADVCAKAEKVKKHTSNALALVNSFVQKRIP
Sbjct: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 211

[Close]

BLAST of CmaCh04G021160 vs. NCBI nr
Match: XP_023532575.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.4 bits (1012), Expect = 6.1e-106
Identity = 206/212 (97.17%), Postives = 207/212 (97.64%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           MKPQS LILSLLFAATLCLLRQVP  A AAADAGTSVNATAN TEFIRSSCVITLYPDVC
Sbjct: 1   MKPQSSLILSLLFAATLCLLRQVP--AVAAADAGTSVNATANSTEFIRSSCVITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           YASLSRYANDIQQDPASLTR+AITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF
Sbjct: 61  YASLSRYANDIQQDPASLTRIAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           MKADVCAKAEKVKKHTSNALALVNSFVQKRIP
Sbjct: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 210

[Close]

BLAST of CmaCh04G021160 vs. NCBI nr
Match: XP_038906561.1 (21 kDa protein [Benincasa hispida])

HSP 1 Score: 365.2 bits (936), Expect = 3.9e-97
Identity = 192/212 (90.57%), Postives = 197/212 (92.92%), Query Frame = 0


Query: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60
           MKPQ PLILSLLFAATL LL  VP  AAAA DAG S NATAN TEFIRSSC ITLYPDVC
Sbjct: 1   MKPQRPLILSLLFAATLFLLCPVP--AAAADDAGISANATANCTEFIRSSCGITLYPDVC 60

Query: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120
           Y SLSRYANDIQQDPASLTR+AITISLANSRRMA+YVSNLSHVGD+GADHRAASALHDCF
Sbjct: 61  YTSLSRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDFGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180
           TNFDDAVDEIRGSL QMRQINDVD PSF+FQMSNVQTWMSAALTDQETCTDGFEDVADGP
Sbjct: 121 TNFDDAVDEIRGSLKQMRQINDVDAPSFKFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 213
           MK DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 MKEDVCAKAEKVKKHTSNALALVNSFVEKRIP 210

[Close]

BLAST of CmaCh04G021160 vs. TAIR 10
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 184.5 bits (467), Expect = 8.9e-47
Identity = 93/182 (51.10%), Postives = 135/182 (74.18%), Query Frame = 0


Query: 36  SVNATANGTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMAS 95
           +V++ ++ ++FIR+SC  TLYPD+C++SLS +++ +  DPA L R AI+++L  +  +AS
Sbjct: 23  TVHSQSDDSDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARAAISVTLTKTLDLAS 82

Query: 96  YVSNLSHV----GDYGADH-RAASALHDCFTNFDDAVDEIRGSLNQMRQ-INDVDVPSFR 155
           Y++N++ +     + GA H  AA+  HDCF N  DAV+E++GS+ QMR+ ++   + SFR
Sbjct: 83  YLANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQMRELVSTGSLESFR 142

Query: 156 FQMSNVQTWMSAALTDQETCTDGFEDVADGPMKADVCAKAEKVKKHTSNALALVNSFVQK 212
           FQMSNVQTW+SAALTD+ETCTDGF+D+ D P K D+CA+ + VKK TSNALALVN  V K
Sbjct: 143 FQMSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDK 202

[Close]

BLAST of CmaCh04G021160 vs. TAIR 10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 9.2e-44
Identity = 96/185 (51.89%), Postives = 128/185 (69.19%), Query Frame = 0


Query: 40  TANGTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSN 99
           T N  +FIR+SC  TLYPDVC+ SLS YA+ +Q  PA L ++AI +SL+ ++  A+++S 
Sbjct: 38  TTNDLDFIRTSCNATLYPDVCFTSLSGYASAVQDSPARLAKLAIGVSLSQAKSTAAFLSK 97

Query: 100 LSH-VGDYGAD-HRAASA-LHDCFTNFDDAVDEIRGSLNQMRQIND--------VDVPSF 159
           LS     Y  D H+ ASA + DC +N +DAVDE+RGSL Q+R +N           V +F
Sbjct: 98  LSRSAAKYSGDGHQTASAVIRDCVSNVEDAVDEMRGSLRQLRDMNGRGGGTAARRSVETF 157

Query: 160 RFQMSNVQTWMSAALTDQETCTDGFEDVADGPM-KADVCAKAEKVKKHTSNALALVNSFV 213
           RFQMSNVQTWMSAALTD++TCTDGFED+ +G + K  VC + E+VK+ TSNALALVN++ 
Sbjct: 158 RFQMSNVQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRLEEVKRLTSNALALVNTYA 217

[Close]

BLAST of CmaCh04G021160 vs. TAIR 10
Match: AT1G14890.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 170.2 bits (430), Expect = 1.7e-42
Identity = 89/170 (52.35%), Postives = 122/170 (71.76%), Query Frame = 0


Query: 40  TANGTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSN 99
           T +  +FIR+SC  TLYPDVCY SL+ YA+ +Q +PA L ++AI +SL+ ++  A+Y+S 
Sbjct: 52  TTDDLDFIRTSCNTTLYPDVCYTSLAGYASAVQDNPARLAKLAIGVSLSRAKYTAAYLSK 111

Query: 100 LSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQIND--VDVPSFRFQMSNVQT 159
           LS          A++A+HDC +N  DAVD++RGSL Q+R++N      P+FRFQMSNVQT
Sbjct: 112 LSR-------RAASAAVHDCVSNVGDAVDQMRGSLRQLREMNHRRPGDPAFRFQMSNVQT 171

Query: 160 WMSAALTDQETCTDGF-EDVADGPMKADVCAKAEKVKKHTSNALALVNSF 207
           WMSAALTD+ETCTDG  E++ DG  K  +C +   VK+ TSNALALVN++
Sbjct: 172 WMSAALTDEETCTDGVTEEMEDGETKTAICDRVADVKRFTSNALALVNTY 214

[Close]

BLAST of CmaCh04G021160 vs. TAIR 10
Match: AT1G70720.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 1.9e-41
Identity = 89/174 (51.15%), Postives = 120/174 (68.97%), Query Frame = 0


Query: 41  ANGTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNL 100
           +NG++FIR +C  TLYPD+C+++LS +AN IQ D   L RVAI+++L N+  + SY+ N 
Sbjct: 31  SNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNRLARVAISLTLHNTLHLLSYLQNA 90

Query: 101 SHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDV--DVPSFRFQMSNVQTW 160
                Y  DH     L DCF N  DAVD +RGS+ QM+++      + SFRFQMSNV+TW
Sbjct: 91  -----YNRDH-PTPVLRDCFENLKDAVDGMRGSMKQMKELVSASGSIESFRFQMSNVKTW 150

Query: 161 MSAALTDQETCTDGFEDV-ADGPMKADVCAKAEKVKKHTSNALALVNSFVQKRI 212
           +SAALTD+ TCTDGF+DV  D  +K DVC++ + VKK TSNALALVN +  + I
Sbjct: 151 LSAALTDEYTCTDGFKDVHEDDSIKDDVCSRVDDVKKLTSNALALVNRYADESI 198

[Close]

BLAST of CmaCh04G021160 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 8.3e-29
Identity = 73/181 (40.33%), Postives = 101/181 (55.80%), Query Frame = 0


Query: 30  AADAGTSVNATANGTEFIRSSCVITLYPDVCYASLSRYANDIQQDPASLTRVAITISLAN 89
           AA A +   A+     FI+SSC  T YP +C  SLS YANDIQ  P  L   AI ++L+ 
Sbjct: 22  AATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSR 81

Query: 90  SRRMASYVSNLSHVGDYGADHRAASALHDCFTNFDDAVDEIRGSLNQMRQINDV-DVPSF 149
           ++    +VS L+ +   G   R   A+ DC    +D VD +  S+ +++      D   F
Sbjct: 82  AQSTKLFVSRLTRM--KGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQF 141

Query: 150 RFQMSNVQTWMSAALTDQETCTDGFED-VADGPMKADVCAKAEKVKKHTSNALALVNSFV 209
            + MSN QTW SAALTD+ TC+DGF   V DG +K  V A+   V   TSNAL+L+N+F 
Sbjct: 142 AYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFA 200

The following BLAST results are available for this feature:

BLAST of CmaCh04G021160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	2.4e-28	39.30	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	1.4e-25	38.50	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	1.1e-22	33.50	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9STY5	7.4e-22	35.71	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q84WE4	1.4e-20	36.84	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]

BLAST of CmaCh04G021160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JSR9	1.2e-110	100.00	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111487516 PE=4 SV=1	[more]
A0A6J1HFF2	1.0e-106	97.64	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111463076 PE=4 SV=1	[more]
A0A5A7TTZ6	9.2e-92	86.79	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00440...	[more]
A0A1S3CSS6	9.2e-92	86.79	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103504548 PE=4 SV=1	[more]
A0A6J1FD40	1.6e-91	85.38	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111444672 PE=4 SV=1	[more]

BLAST of CmaCh04G021160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022990704.1	2.4e-110	100.00	21 kDa protein-like [Cucurbita maxima]	[more]
XP_022962658.1	2.1e-106	97.64	21 kDa protein-like [Cucurbita moschata]	[more]
KAG6601869.1	2.1e-106	97.64	hypothetical protein SDJN03_07102, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023532575.1	6.1e-106	97.17	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_038906561.1	3.9e-97	90.57	21 kDa protein [Benincasa hispida]	[more]

BLAST of CmaCh04G021160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23205.1	8.9e-47	51.10	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G01610.1	9.2e-44	51.89	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G14890.1	1.7e-42	52.35	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G70720.1	1.9e-41	51.15	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	8.3e-29	40.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 42..202 e-value: 1.9E-42 score: 157.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..204 e-value: 1.9E-31 score: 106.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 46..202 e-value: 1.2E-38 score: 132.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 41..206 e-value: 1.7E-39 score: 137.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 45..206
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 16..209
None	No IPR available	PANTHER	PTHR31080:SF64	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 16..209
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 49..206 e-value: 1.45273E-45 score: 146.429

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G021160.1	CmaCh04G021160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity