CmaCh04G021050 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G021050
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Polcalcin Jun o
Location	Cma_Chr04: 14718505 .. 14719074 (+)
RNA-Seq Expression	CmaCh04G021050
Synteny	CmaCh04G021050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCCGATTCCTCCAGCGATTCACCGCTCGGATCCACCACGGTTCCGATTCTCGGATCTCGAACTCCGTCTTTCAATTCCCGAGCGCAAATCGCGGAACTCGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGTAAGATCTGCTCCTCTGAACTCGGTTCCATTATGGGGAGCCTAGGCCAGCCGGCCACGGAGGAAGAGCTTCAGAACATGATCAAGGAAGTCGATGCCGACGGCGATGGCTACATTGACCTCCATGAGTTCATTGAACTCAACACCAAGGGAGTCGATTCCGACGAGGTGCTTAAGAATCTGAAGGACGCATTCTCCGTCTATGACCTTGACGGCAACGGATCCATCACGGCGGAGGAATTGCACGAGGTCTTGAAGAGTTTGGGCGACGACTGCTCGCTGGCGGATTGCCGGAACATGATCAGCGGCGTCGACAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGCCATGATGATGAGCGGTTCTCGATCTCACGGCATCAGCGATTAG

mRNA sequence

ATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCCGATTCCTCCAGCGATTCACCGCTCGGATCCACCACGGTTCCGATTCTCGGATCTCGAACTCCGTCTTTCAATTCCCGAGCGCAAATCGCGGAACTCGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGTAAGATCTGCTCCTCTGAACTCGGTTCCATTATGGGGAGCCTAGGCCAGCCGGCCACGGAGGAAGAGCTTCAGAACATGATCAAGGAAGTCGATGCCGACGGCGATGGCTACATTGACCTCCATGAGTTCATTGAACTCAACACCAAGGGAGTCGATTCCGACGAGGTGCTTAAGAATCTGAAGGACGCATTCTCCGTCTATGACCTTGACGGCAACGGATCCATCACGGCGGAGGAATTGCACGAGGTCTTGAAGAGTTTGGGCGACGACTGCTCGCTGGCGGATTGCCGGAACATGATCAGCGGCGTCGACAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGCCATGATGATGAGCGGTTCTCGATCTCACGGCATCAGCGATTAG

Coding sequence (CDS)

ATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCCGATTCCTCCAGCGATTCACCGCTCGGATCCACCACGGTTCCGATTCTCGGATCTCGAACTCCGTCTTTCAATTCCCGAGCGCAAATCGCGGAACTCGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGTAAGATCTGCTCCTCTGAACTCGGTTCCATTATGGGGAGCCTAGGCCAGCCGGCCACGGAGGAAGAGCTTCAGAACATGATCAAGGAAGTCGATGCCGACGGCGATGGCTACATTGACCTCCATGAGTTCATTGAACTCAACACCAAGGGAGTCGATTCCGACGAGGTGCTTAAGAATCTGAAGGACGCATTCTCCGTCTATGACCTTGACGGCAACGGATCCATCACGGCGGAGGAATTGCACGAGGTCTTGAAGAGTTTGGGCGACGACTGCTCGCTGGCGGATTGCCGGAACATGATCAGCGGCGTCGACAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGCCATGATGATGAGCGGTTCTCGATCTCACGGCATCAGCGATTAG

Protein sequence

MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGSRSHGISD

Homology

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BLAST of CmaCh04G021050 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 5.0e-54
Identity = 107/186 (57.53%), Postives = 139/186 (74.73%), Query Frame = 0


Query: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTP----SFNSRAQIAELEQVFKKFDVNGDGKIC 65
           +F++ + S+  S S +G      +G+ +P    + + + +I ELE VFKKFDVNGDGKI 
Sbjct: 1   MFNKNQGSNGGSSSNVG------IGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKIS 60

Query: 66  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDA 125
           S ELG+IM SLG    EEEL+  I E+D  GDGYI+  EF+ELNTKG+D ++VL+NLKDA
Sbjct: 61  SKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 120

Query: 126 FSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSG 185
           FSVYD+DGNGSI+AEELHEVL+SLGD+CS+A+CR MI GVD++GDG I F+EFK MM  G
Sbjct: 121 FSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 180

Query: 186 SRSHGI 188
           SR   +
Sbjct: 181 SRRDNV 180

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BLAST of CmaCh04G021050 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.1e-36
Identity = 87/178 (48.88%), Postives = 114/178 (64.04%), Query Frame = 0


Query: 5   SLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSE 64
           +LF R+    S S SP      P  G  +P+        E+E+VF+KFD NGDG+I  SE
Sbjct: 8   ALFRRR----SGSKSPPLPQADPASGGGSPAPTPE---EEMERVFRKFDANGDGRISRSE 67

Query: 65  LGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELN-TKGVDSDEVLKNLKDAFS 124
           LG++  SLG  AT++EL  M+ E DADGDG+I L EF  LN T   D+  V ++L+ AF 
Sbjct: 68  LGALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFR 127

Query: 125 VYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSG 182
           V+D DGNG+I+A EL  VL  LG+  ++  CR MI GVD+NGDG+ISF+EFK MM  G
Sbjct: 128 VFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGG 178

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BLAST of CmaCh04G021050 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 152.1 bits (383), Expect = 6.1e-36
Identity = 74/139 (53.24%), Postives = 101/139 (72.66%), Query Frame = 0


Query: 42  IAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEF 101
           + ELE+VFKKFD NGDGKI  SEL  I+ SLG    E E++ M++E DADGDGY+ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 102 IELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGV 161
           ++LN KG      +K+LK+AF V+D D NGSI+A EL   L+S+G+ C++ + +N+I  V
Sbjct: 84  VDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 162 DRNGDGMISFDEFKAMMMS 181
           D+NGDG+IS +EF+ MM S
Sbjct: 144 DKNGDGLISVEEFQTMMTS 158

[Close]

BLAST of CmaCh04G021050 vs. ExPASy Swiss-Prot
Match: A0T2M3 (Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 4.0e-35
Identity = 71/139 (51.08%), Postives = 100/139 (71.94%), Query Frame = 0


Query: 42  IAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEF 101
           + ELE+VFKKFD NGDGKI  SEL  I+ S+G    E E++ M++E D DGDGY+ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSMGSEVDEAEVKAMMEEADTDGDGYVSLQEF 83

Query: 102 IELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGV 161
           ++LN KG      +K+LK+AF V+D D NG+I+  EL E LKS+G+ C++ + +N+I  V
Sbjct: 84  VDLNIKGA----TVKDLKNAFKVFDRDCNGTISPAELCETLKSVGEPCTIEESKNIIHNV 143

Query: 162 DRNGDGMISFDEFKAMMMS 181
           D+NGDG+I+ +EF+ MM S
Sbjct: 144 DKNGDGLINVEEFQTMMTS 158

[Close]

BLAST of CmaCh04G021050 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.8e-33
Identity = 69/151 (45.70%), Postives = 105/151 (69.54%), Query Frame = 0


Query: 31  SRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDA 90
           S+  S N    + ++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 91  DGDGYIDLHEFIEL--NTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDD 150
           DG+G+IDL EF+ L   +    ++  +++LK+AF +YDLD NG I+A ELH V+K+LG+ 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 151 CSLADCRNMISGVDRNGDGMISFDEFKAMMM 180
           CS+ DC+ MI+ VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

[Close]

BLAST of CmaCh04G021050 vs. ExPASy TrEMBL
Match: A0A6J1JTS9 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487441 PE=4 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.8e-99
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGISD 190
           GSRSHGISD
Sbjct: 181 GSRSHGISD 189

[Close]

BLAST of CmaCh04G021050 vs. ExPASy TrEMBL
Match: A0A6J1HID9 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111463873 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.1e-99
Identity = 188/189 (99.47%), Postives = 189/189 (100.00%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGISD 190
           GSRSHG+SD
Sbjct: 181 GSRSHGLSD 189

[Close]

BLAST of CmaCh04G021050 vs. ExPASy TrEMBL
Match: A0A6J1CZ20 (probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC111015568 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 6.5e-89
Identity = 169/186 (90.86%), Postives = 178/186 (95.70%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
            SSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKD
Sbjct: 61  SSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180

Query: 181 GSRSHG 187
           GSRSHG
Sbjct: 181 GSRSHG 186

[Close]

BLAST of CmaCh04G021050 vs. ExPASy TrEMBL
Match: A0A0A0KST2 (Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.3e-85
Identity = 167/188 (88.83%), Postives = 174/188 (92.55%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKS SS+DSPLGSTT+PILGSRT       QIAELEQVF KFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRT-------QIAELEQVFNKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVD+NGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 180

Query: 181 GSRSHGIS 189
           GSRS G +
Sbjct: 181 GSRSQGFN 181

[Close]

BLAST of CmaCh04G021050 vs. ExPASy TrEMBL
Match: A0A5A7TS48 (Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3042G00010 PE=4 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 6.3e-84
Identity = 164/188 (87.23%), Postives = 172/188 (91.49%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKS  S+DSPLGSTT+PI GSRT       QIAELEQVF KFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRT-------QIAELEQVFNKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMGSLGQPATEEEL NMIKEVDADGDGYIDL EFIELNTKGVDS+EVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVD+NGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 180

Query: 181 GSRSHGIS 189
           GSRSHG +
Sbjct: 181 GSRSHGFN 181

[Close]

BLAST of CmaCh04G021050 vs. NCBI nr
Match: XP_022990613.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 371.7 bits (953), Expect = 3.8e-99
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGISD 190
           GSRSHGISD
Sbjct: 181 GSRSHGISD 189

[Close]

BLAST of CmaCh04G021050 vs. NCBI nr
Match: XP_022963580.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 6.4e-99
Identity = 188/189 (99.47%), Postives = 189/189 (100.00%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGISD 190
           GSRSHG+SD
Sbjct: 181 GSRSHGLSD 189

[Close]

BLAST of CmaCh04G021050 vs. NCBI nr
Match: XP_023513887.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.4 bits (947), Expect = 1.9e-98
Identity = 187/189 (98.94%), Postives = 188/189 (99.47%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGISD 190
           GSRSHG+ D
Sbjct: 181 GSRSHGLGD 189

[Close]

BLAST of CmaCh04G021050 vs. NCBI nr
Match: KAG6601861.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032560.1 putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 369.0 bits (946), Expect = 2.4e-98
Identity = 187/189 (98.94%), Postives = 188/189 (99.47%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
           CSSELGSIMG LGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD
Sbjct: 61  CSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRSHGISD 190
           GSRSHG+SD
Sbjct: 181 GSRSHGLSD 189

[Close]

BLAST of CmaCh04G021050 vs. NCBI nr
Match: XP_022146331.1 (probable calcium-binding protein CML25 [Momordica charantia])

HSP 1 Score: 336.7 bits (862), Expect = 1.3e-88
Identity = 169/186 (90.86%), Postives = 178/186 (95.70%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120
            SSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKD
Sbjct: 61  SSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKD 120

Query: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180

Query: 181 GSRSHG 187
           GSRSHG
Sbjct: 181 GSRSHG 186

[Close]

BLAST of CmaCh04G021050 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 212.2 bits (539), Expect = 3.5e-55
Identity = 107/186 (57.53%), Postives = 139/186 (74.73%), Query Frame = 0


Query: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTP----SFNSRAQIAELEQVFKKFDVNGDGKIC 65
           +F++ + S+  S S +G      +G+ +P    + + + +I ELE VFKKFDVNGDGKI 
Sbjct: 1   MFNKNQGSNGGSSSNVG------IGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKIS 60

Query: 66  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDA 125
           S ELG+IM SLG    EEEL+  I E+D  GDGYI+  EF+ELNTKG+D ++VL+NLKDA
Sbjct: 61  SKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 120

Query: 126 FSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSG 185
           FSVYD+DGNGSI+AEELHEVL+SLGD+CS+A+CR MI GVD++GDG I F+EFK MM  G
Sbjct: 121 FSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 180

Query: 186 SRSHGI 188
           SR   +
Sbjct: 181 SRRDNV 180

[Close]

BLAST of CmaCh04G021050 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 143.3 bits (360), Expect = 2.0e-34
Identity = 69/151 (45.70%), Postives = 105/151 (69.54%), Query Frame = 0


Query: 31  SRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDA 90
           S+  S N    + ++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 91  DGDGYIDLHEFIEL--NTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDD 150
           DG+G+IDL EF+ L   +    ++  +++LK+AF +YDLD NG I+A ELH V+K+LG+ 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 151 CSLADCRNMISGVDRNGDGMISFDEFKAMMM 180
           CS+ DC+ MI+ VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

[Close]

BLAST of CmaCh04G021050 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 142.9 bits (359), Expect = 2.6e-34
Identity = 69/142 (48.59%), Postives = 102/142 (71.83%), Query Frame = 0


Query: 44  ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIE 103
           ++++VF++FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDL EF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 104 LNTKGV----DSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMIS 163
           L   G+    ++   + +LK+AF +YDLDGNG I+A+ELH V+K+LG+ CS+ DC+ MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 164 GVDRNGDGMISFDEFKAMMMSG 182
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of CmaCh04G021050 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.4 bits (337), Expect = 9.4e-32
Identity = 72/156 (46.15%), Postives = 95/156 (60.90%), Query Frame = 0


Query: 27  PILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIK 86
           P     TP+    A   EL++VF +FD NGDGKI   ELG +  ++G   TE EL  +++
Sbjct: 6   PETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLE 65

Query: 87  EVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLG 146
           EVD D DGYI+L EF  L      + E+    +DAF +YD D NG I+A ELH+VL  LG
Sbjct: 66  EVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLNRLG 125

Query: 147 DDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGS 183
             CS+ DC  MI  VD +GDG ++F+EF+ MM S S
Sbjct: 126 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

[Close]

BLAST of CmaCh04G021050 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.4 bits (337), Expect = 9.4e-32
Identity = 72/156 (46.15%), Postives = 95/156 (60.90%), Query Frame = 0


Query: 27  PILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIK 86
           P     TP+    A   EL++VF +FD NGDGKI   ELG +  ++G   TE EL  +++
Sbjct: 6   PETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLE 65

Query: 87  EVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVYDLDGNGSITAEELHEVLKSLG 146
           EVD D DGYI+L EF  L      + E+    +DAF +YD D NG I+A ELH+VL  LG
Sbjct: 66  EVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLNRLG 125

Query: 147 DDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGS 183
             CS+ DC  MI  VD +GDG ++F+EF+ MM S S
Sbjct: 126 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

The following BLAST results are available for this feature:

BLAST of CmaCh04G021050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYK2	5.0e-54	57.53	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q8RZB5	2.1e-36	48.88	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
O64943	6.1e-36	53.24	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
A0T2M3	4.0e-35	51.08	Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1	[more]
Q9C8Y1	2.8e-33	45.70	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of CmaCh04G021050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JTS9	1.8e-99	100.00	probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114874...	[more]
A0A6J1HID9	3.1e-99	99.47	probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1CZ20	6.5e-89	90.86	probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0KST2	3.3e-85	88.83	Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1	[more]
A0A5A7TS48	6.3e-84	87.23	Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of CmaCh04G021050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022990613.1	3.8e-99	100.00	probable calcium-binding protein CML25 [Cucurbita maxima]	[more]
XP_022963580.1	6.4e-99	99.47	probable calcium-binding protein CML25 [Cucurbita moschata]	[more]
XP_023513887.1	1.9e-98	98.94	probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo]	[more]
KAG6601861.1	2.4e-98	98.94	putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022146331.1	1.3e-88	90.86	probable calcium-binding protein CML25 [Momordica charantia]	[more]

BLAST of CmaCh04G021050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	3.5e-55	57.53	EF hand calcium-binding protein family	[more]
AT1G66400.1	2.0e-34	45.70	calmodulin like 23	[more]
AT5G37770.1	2.6e-34	48.59	EF hand calcium-binding protein family	[more]
AT1G18210.1	9.4e-32	46.15	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	9.4e-32	46.15	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 80..108 e-value: 6.7E-5 score: 32.3 coord: 44..72 e-value: 4.4E-6 score: 36.2 coord: 117..145 e-value: 2.0E-5 score: 34.0 coord: 153..181 e-value: 1.9E-5 score: 34.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 44..103 e-value: 3.5E-13 score: 49.8 coord: 117..178 e-value: 4.1E-12 score: 46.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 113..148 score: 15.182896
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 149..184 score: 12.78389
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 40..75 score: 15.071314
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 76..111 score: 13.537066
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 118..179 e-value: 1.64828E-20 score: 78.7437
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 44..104 e-value: 1.02353E-19 score: 76.8177
None	No IPR available	GENE3D	1.10.238.10		coord: 31..106 e-value: 7.7E-25 score: 89.0 coord: 107..185 e-value: 4.2E-25 score: 89.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..33
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..33
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 27..184
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 27..184
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 126..138
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 53..65
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 89..101
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 162..174
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 32..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G021050.1	CmaCh04G021050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding