CmaCh04G021020 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G021020
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description(1->3)-beta-glucan endohydrolase
LocationCma_Chr04: 14700606 .. 14702884 (+)
RNA-Seq ExpressionCmaCh04G021020
SyntenyCmaCh04G021020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGACACCCATGCCTATTGTTTTTGCTAATTATTTCTTGTTTGGGCCATGAAGGTCTTGTAAAGGGTGCTTTAGGGTTTGCGTGTAATTGGGGAACTCGCTCCAGCCATCCACTCCCACCTGATATTGTTGTTAAGTTGATCAAGGATAATGGGTTCAATAAAGTGAAGCTTTTTGAAGCTGATCCTGGAGCTCTTCAGGCTTTAGGAAATTCTGGTCTTCAAGTTATGCTTGGAATCCCTAATGAATTTTTGGCACCGCTTTCAAGTACTGTACGAGTTGCTGAGGAATGGGTAGCCAAAAACGTGTCTTACTTCGTTTCAAATTTTGGTACTGATATCAGGTAATGACTTTAATTAGCCCCTTGTGTTATTTCTGTTAATGTTGATATTGTTAGAAGAGCTTATGCCAATAGAGATAATATTCCTTGAATATAAACCCAACTTTCATCCAATATCTCCCCTCGAACAAAGTATGCCTCCCTTTAATTGTGGTTCGACTCTTTTTGCTTCTTTTGGAGTCTTCGAACATAGTGCACCCATTGTTCAATACTTTAGTCACTTTTTGACTATGTCTTACAACACTTTGTTCGACATTTGAGGATTCTAATGTCATGGCTAAGTTTTAGGGCATGACTCTGATATCATGTTAGTAATCACGGAATCTTCATATCTCCACGATGGTATCATATTGTTCATTTTGAGCATAAACTCTATGGCTTTGAGTTGTGATTCCTCAAAAGGCCTCATGCCAATGGAGGTAGTATTATTTGATTATAAACATATGATTATTCCCTAAATTAGCCGAGGTAAAACTTTCATCGAACTGATATTAGGGTGTTAGGAATTAAACTTGCATATATGGGTTCGATTGCGTAGGTATGTGGCTGTGGGTAACGAACCTTTCCTAAACGCCTACAACGGTTCATTTCTTCAAACCACGTTCCCGGCTATGCAAAACGTACAAGCCGCCCTAATAAAAGCCGGTTTGGGTCGACAAGTGAAAGTCACAGTCCCATTAAACGCCGACGTTTATGAGACCGAGAATGGGCTCCCTTCCGGCGGCAACTTCCGGCCGGACATCCGTGATCTTATGGTTAATATCATTAAGTTCCTCAGCAACAACGCCGCTCCTCTCACCATCAACATCTACCCCTTCCTTAGCCTCAACGCTGATCCACATTTCCCCAAGGAGTATGCCTTCTTCAACGGCAATGCCGCCCCCGTTATCGATGGCTCGATCTCTTACACCAATGTTCTTGATGCAAATTTTGACACTCTCGTCTCCGCTCTTGAGAAGAATGGCTTTTCCAACATGCCTATCATTATTGGAGAGGTCGGGTGGCCGACCGATGGAGATCCTAGCGCCAATAAAGCTAATGCTCAAAGATTCAATCAAGGTAGATTAAATTACTAGTTCCAAGTATAAATTCTTTAGAATATTAGACGAAGGTTGATATAACAACTAAATTGCTTGTATGATTTATCGATTACTAGGCCTAGTCGAACGCATCAATCGTCGGCAAGGCACACCGAAGAGGCCCGTGCCCGTAGACACCTACATCTTCGCAATCATCGACGAGGACGCAAAGAGCATTCAACCGGGGAGTTTTGAGCGACATTGGGGAGTTTTCAATAACGATGGAACAATCAAGTACTCTTTGAACATGGGGAAGGGGAGGTCCTTGGTTCCAGCCAAAGGAGTTCGATACTTGCCAAAGGAGTGGTGCGTGATGTCACCGCAAGCTAGTCTTTCGGATCCCAATCTTCCGAATAGCGTTGCATATGCGTGCGACCATGCTGATTGCACAAGCCTTAGCTACGGCTCCTCCTGCAGCCAACTTGATGCGAAAAGCAACGTTTCATATGCATTCAATGCCTATTTTCAAACAATGAACCAGAGTTCTTCCGCATGCAAATTTTCGAACCTAGCAACGGTTACCACCGTCGATCCCTCTCCGCCTAATCAGGGCAACACCAGGGATGGCTGCCGATTTGCGATTATGATCGATACTTCCAAAAGGCATCGACTTGCAAGACCGCCCAGAACTTCCTCAGCTTCACCCACAATAGCCCACATTAGCTGCTGCCATGGGATCACTCTACTTCTCGCGCTCTTCGTTTTGATTCTCAAATATTGATCTTTTATGCATCAACATTTGTTCTCAAGCCATTGGTTTGATTTTTTATGCCCACAAAATATACAATTTGTTTAAAAAAAGAAAAAGAAAAAGAAAAGAAAAAAAAAAGGGGGATACGTCTTAATACTTTTAATAAAG

mRNA sequence

ATGGGACACCCATGCCTATTGTTTTTGCTAATTATTTCTTGTTTGGGCCATGAAGGTCTTGTAAAGGGTGCTTTAGGGTTTGCGTGTAATTGGGGAACTCGCTCCAGCCATCCACTCCCACCTGATATTGTTGTTAAGTTGATCAAGGATAATGGGTTCAATAAAGTGAAGCTTTTTGAAGCTGATCCTGGAGCTCTTCAGGCTTTAGGAAATTCTGGTCTTCAAGTTATGCTTGGAATCCCTAATGAATTTTTGGCACCGCTTTCAAGTACTGTACGAGTTGCTGAGGAATGGGTAGCCAAAAACGTGTCTTACTTCGTTTCAAATTTTGGTACTGATATCAGGTATGTGGCTGTGGGTAACGAACCTTTCCTAAACGCCTACAACGGTTCATTTCTTCAAACCACGTTCCCGGCTATGCAAAACGTACAAGCCGCCCTAATAAAAGCCGGTTTGGGTCGACAAGTGAAAGTCACAGTCCCATTAAACGCCGACGTTTATGAGACCGAGAATGGGCTCCCTTCCGGCGGCAACTTCCGGCCGGACATCCGTGATCTTATGGTTAATATCATTAAGTTCCTCAGCAACAACGCCGCTCCTCTCACCATCAACATCTACCCCTTCCTTAGCCTCAACGCTGATCCACATTTCCCCAAGGAGTATGCCTTCTTCAACGGCAATGCCGCCCCCGTTATCGATGGCTCGATCTCTTACACCAATGTTCTTGATGCAAATTTTGACACTCTCGTCTCCGCTCTTGAGAAGAATGGCTTTTCCAACATGCCTATCATTATTGGAGAGGTCGGGTGGCCGACCGATGGAGATCCTAGCGCCAATAAAGCTAATGCTCAAAGATTCAATCAAGGCCTAGTCGAACGCATCAATCGTCGGCAAGGCACACCGAAGAGGCCCGTGCCCGTAGACACCTACATCTTCGCAATCATCGACGAGGACGCAAAGAGCATTCAACCGGGGAGTTTTGAGCGACATTGGGGAGTTTTCAATAACGATGGAACAATCAAGTACTCTTTGAACATGGGGAAGGGGAGGTCCTTGGTTCCAGCCAAAGGAGTTCGATACTTGCCAAAGGAGTGGTGCGTGATGTCACCGCAAGCTAGTCTTTCGGATCCCAATCTTCCGAATAGCGTTGCATATGCGTGCGACCATGCTGATTGCACAAGCCTTAGCTACGGCTCCTCCTGCAGCCAACTTGATGCGAAAAGCAACGTTTCATATGCATTCAATGCCTATTTTCAAACAATGAACCAGAGTTCTTCCGCATGCAAATTTTCGAACCTAGCAACGGTTACCACCGTCGATCCCTCTCCGCCTAATCAGGGCAACACCAGGGATGGCTGCCGATTTGCGATTATGATCGATACTTCCAAAAGGCATCGACTTGCAAGACCGCCCAGAACTTCCTCAGCTTCACCCACAATAGCCCACATTAGCTGCTGCCATGGGATCACTCTACTTCTCGCGCTCTTCGTTTTGATTCTCAAATATTGATCTTTTATGCATCAACATTTGTTCTCAAGCCATTGGTTTGATTTTTTATGCCCACAAAATATACAATTTGTTTAAAAAAAGAAAAAGAAAAAGAAAAGAAAAAAAAAAGGGGGATACGTCTTAATACTTTTAATAAAG

Coding sequence (CDS)

ATGGGACACCCATGCCTATTGTTTTTGCTAATTATTTCTTGTTTGGGCCATGAAGGTCTTGTAAAGGGTGCTTTAGGGTTTGCGTGTAATTGGGGAACTCGCTCCAGCCATCCACTCCCACCTGATATTGTTGTTAAGTTGATCAAGGATAATGGGTTCAATAAAGTGAAGCTTTTTGAAGCTGATCCTGGAGCTCTTCAGGCTTTAGGAAATTCTGGTCTTCAAGTTATGCTTGGAATCCCTAATGAATTTTTGGCACCGCTTTCAAGTACTGTACGAGTTGCTGAGGAATGGGTAGCCAAAAACGTGTCTTACTTCGTTTCAAATTTTGGTACTGATATCAGGTATGTGGCTGTGGGTAACGAACCTTTCCTAAACGCCTACAACGGTTCATTTCTTCAAACCACGTTCCCGGCTATGCAAAACGTACAAGCCGCCCTAATAAAAGCCGGTTTGGGTCGACAAGTGAAAGTCACAGTCCCATTAAACGCCGACGTTTATGAGACCGAGAATGGGCTCCCTTCCGGCGGCAACTTCCGGCCGGACATCCGTGATCTTATGGTTAATATCATTAAGTTCCTCAGCAACAACGCCGCTCCTCTCACCATCAACATCTACCCCTTCCTTAGCCTCAACGCTGATCCACATTTCCCCAAGGAGTATGCCTTCTTCAACGGCAATGCCGCCCCCGTTATCGATGGCTCGATCTCTTACACCAATGTTCTTGATGCAAATTTTGACACTCTCGTCTCCGCTCTTGAGAAGAATGGCTTTTCCAACATGCCTATCATTATTGGAGAGGTCGGGTGGCCGACCGATGGAGATCCTAGCGCCAATAAAGCTAATGCTCAAAGATTCAATCAAGGCCTAGTCGAACGCATCAATCGTCGGCAAGGCACACCGAAGAGGCCCGTGCCCGTAGACACCTACATCTTCGCAATCATCGACGAGGACGCAAAGAGCATTCAACCGGGGAGTTTTGAGCGACATTGGGGAGTTTTCAATAACGATGGAACAATCAAGTACTCTTTGAACATGGGGAAGGGGAGGTCCTTGGTTCCAGCCAAAGGAGTTCGATACTTGCCAAAGGAGTGGTGCGTGATGTCACCGCAAGCTAGTCTTTCGGATCCCAATCTTCCGAATAGCGTTGCATATGCGTGCGACCATGCTGATTGCACAAGCCTTAGCTACGGCTCCTCCTGCAGCCAACTTGATGCGAAAAGCAACGTTTCATATGCATTCAATGCCTATTTTCAAACAATGAACCAGAGTTCTTCCGCATGCAAATTTTCGAACCTAGCAACGGTTACCACCGTCGATCCCTCTCCGCCTAATCAGGGCAACACCAGGGATGGCTGCCGATTTGCGATTATGATCGATACTTCCAAAAGGCATCGACTTGCAAGACCGCCCAGAACTTCCTCAGCTTCACCCACAATAGCCCACATTAGCTGCTGCCATGGGATCACTCTACTTCTCGCGCTCTTCGTTTTGATTCTCAAATATTGA

Protein sequence

MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVGNEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFRPDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTNVLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGTPKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRYLPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQTMNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTIAHISCCHGITLLLALFVLILKY
Homology
BLAST of CmaCh04G021020 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 8.9e-159
Identity = 267/466 (57.30%), Postives = 340/466 (72.96%), Query Frame = 0

Query: 26  GFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGALQALGNSGLQVMLGIPNEFL 85
           G   NWG+++ HPLPP  VV+L+++NG  KVKLFEAD   L+AL  +G+QVM+GIPN+ L
Sbjct: 26  GIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKALSRTGIQVMVGIPNDLL 85

Query: 86  APLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVGNEPFLNAYNGSFLQTTFPAMQNVQA 145
           APL+ +V  AE WV++NVS  VS+ G DIRYVAVGNEPFL A+NG+F   T PA+QN+Q+
Sbjct: 86  APLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGTFEGITLPALQNIQS 145

Query: 146 ALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFRPDIRDLMVNIIKFLSNNAAPLTINI 205
           A+IKAGL  QVKVTVPLNADVY++ + LPS G+FRP+IRDLM+NI+KFLS+N AP TINI
Sbjct: 146 AIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLMLNIVKFLSDNGAPFTINI 205

Query: 206 YPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTNVLDANFDTLVSALEKNGFSNMPIII 265
           YPF+SL  DP+FP E+AFF+G   P+ D    Y NVLDAN+DTLV +L+KNGF N+ II+
Sbjct: 206 YPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYDTLVWSLQKNGFGNLTIIV 265

Query: 266 GEVGWPTDGDPSANKANAQRFNQGLVERINRRQGTPKRPVPVDTYIFAIIDEDAKSIQPG 325
           GEVGWPTDGD +AN   A+R+NQG + R    +GTP RP  +D Y+F +IDEDAKSIQPG
Sbjct: 266 GEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAMDAYLFGLIDEDAKSIQPG 325

Query: 326 SFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRYLPKEWCVMSPQASLSDPNLPNSVAY 385
           +FERHWG+F  DG  KY L++G G  L+PAK V YL K+WC+++P A+L DP L  SV+Y
Sbjct: 326 NFERHWGIFYIDGQPKYQLSLGSGNGLIPAKDVHYLAKKWCILAPNANLQDPQLGPSVSY 385

Query: 386 ACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQTMNQSSSACKFSNLATVTTVDPSPPN 445
           ACDHADCTSL YGSSC  L+   NVSYAFN+Y+Q  NQ  SACKF  L+ V+T DPS   
Sbjct: 386 ACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSACKFPGLSIVSTRDPSV-- 445

Query: 446 QGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTIAHISCCHGITL 492
                  C+F IMI +      +     + ++  +  +S C  I L
Sbjct: 446 -----GSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSICLYIVL 484

BLAST of CmaCh04G021020 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 558.9 bits (1439), Expect = 5.8e-158
Identity = 264/462 (57.14%), Postives = 342/462 (74.03%), Query Frame = 0

Query: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60
           MG   +L LL ++      L + A     NWGT++SHPLPPDIVV+++++NG  KVKLF+
Sbjct: 1   MGWGSVLLLLAVAL-----LCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFD 60

Query: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120
           A+   L+ALG SG++VM+GIPNE LA L+S+++ AE+WVAKNVS  +S    +IRYVAVG
Sbjct: 61  AEYDTLRALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVG 120

Query: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180
           NEPFL+ YNGS+L TTFPA++N+Q A+IKAGL  QVKVT PLNADVY++    PSGG+FR
Sbjct: 121 NEPFLSTYNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFR 180

Query: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240
            +IRDLM+ I+KFLS N  P T+NIYP++SL  +P FP +YAFF+GNA P+ DG   Y N
Sbjct: 181 ANIRDLMITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYN 240

Query: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300
           + DAN+DTLV ALEKNGF NMPIIIGE+GWPTDGD +AN   A++FNQG +  I+  +GT
Sbjct: 241 MFDANYDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGT 300

Query: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGR--SLVPAKGV 360
           P+RP P+D Y+F++IDEDAKS+QPG FERHWG+F  DG  KY+LN+G     +L+ AKGV
Sbjct: 301 PRRPGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNLGTTNTGALIQAKGV 360

Query: 361 RYLPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYF 420
           RYL ++WCVM P   L DP +  +V+YAC   DCTSL  G+SC+ LD K N+SYAFN+Y+
Sbjct: 361 RYLERKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYY 420

Query: 421 QTMNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMID 461
           Q  +Q  +ACKF N++ VT  DPS          CRF IMI+
Sbjct: 421 QIQDQLDTACKFPNISEVTKTDPSTGT-------CRFPIMIE 450

BLAST of CmaCh04G021020 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 466.1 bits (1198), Expect = 5.1e-130
Identity = 237/467 (50.75%), Postives = 310/467 (66.38%), Query Frame = 0

Query: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60
           M +   L +  +  +GH G++   LG   NWGT ++H LPP  VV+++KDN  NKVKLF+
Sbjct: 1   MSNLLALVVGFVIVIGHLGILVNGLG--VNWGTMATHKLPPKTVVQMLKDNNINKVKLFD 60

Query: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120
           AD   + AL  SGL+VM+ IPN+ L  ++S  R A++WV KNV+ +  + G +I +VAVG
Sbjct: 61  ADETTMGALAGSGLEVMVAIPNDQLKVMTSYDR-AKDWVRKNVTRYNFDGGVNITFVAVG 120

Query: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYET--ENGLPSGGN 180
           NEPFL +YNGSF+  TFPA+ N+Q AL +AGLG  VK TVPLNADVY++   N +PS G 
Sbjct: 121 NEPFLKSYNGSFINLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGR 180

Query: 181 FRPDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISY 240
           FRPDI   M  I+ FL  N AP+TINIYPFLSL  +  FP  YAFF+G A P+ D  I Y
Sbjct: 181 FRPDIIGQMTQIVDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDG-AEPINDNGIDY 240

Query: 241 TNVLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQ 300
           TNV DANFDTLVS+L+  G  +MPII+GEVGWPT+GD  AN  +A RF  GL+ R+   +
Sbjct: 241 TNVFDANFDTLVSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNK 300

Query: 301 GTPKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNM---GKGRSLVPA 360
           GTP RP  ++ Y+F ++DEDAKSI PG FERHWG+F  DG  K+ +++   G+ + L+ A
Sbjct: 301 GTPLRPTYIEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGA 360

Query: 361 KGVRYLPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFN 420
           + V YLP +WC  +P+A      L  ++ YAC  +DCT+L YGSSC+ LDA  N SYAFN
Sbjct: 361 QNVPYLPNKWCTFNPEAK-DLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFN 420

Query: 421 AYFQTMNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTS 463
            +FQ  NQ  SAC F  LAT+TT       Q  ++  C F I I  S
Sbjct: 421 MFFQVKNQDESACYFQGLATITT-------QNISQGQCNFPIQIVAS 455

BLAST of CmaCh04G021020 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 5.0e-101
Identity = 197/476 (41.39%), Postives = 283/476 (59.45%), Query Frame = 0

Query: 6   LLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGA 65
           LL L + + L   G    A+G   NWGT +SHPLPP  VV+L+K NG  KVKLF+ADP  
Sbjct: 7   LLLLAVTAGLSLTGTTVRAVGI--NWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKV 66

Query: 66  LQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVS-YFVSNFGTDIRYVAVGNEPF 125
           L+AL  S + V +GI N  L  L+++V+VAE WV  NV+ YF       I YVAVG EPF
Sbjct: 67  LRALSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRYFNGGNRVRIEYVAVGEEPF 126

Query: 126 LNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFRPDIR 185
           L +Y   +      A  N+Q AL+KA L  +VKV VP + D + +E+G PS G+FR D+ 
Sbjct: 127 LQSYGNQYKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADLN 186

Query: 186 DLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTNVLDA 245
             M+ ++ FL+ + +P  + I PFLS + + +   +++ F   A    DG  +Y N  D 
Sbjct: 187 KTMIELLSFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFDL 246

Query: 246 NFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGTPKRP 305
           ++DTLVSAL   GFS + I++ ++GWPTDG  +A    A+ F +GL+  + ++  +  RP
Sbjct: 247 SYDTLVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTASLPRP 306

Query: 306 VPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRYLPKE 365
            PV+TYI +++DED +++  G+FERHWGVF  DG  KY+ +    ++ V A+ V+YLP +
Sbjct: 307 -PVETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFNH-KNQVNAQNVQYLPPK 366

Query: 366 WCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQTMNQS 425
           WCV++    LS  N       AC  ADCTS+  G SCS +    NVSYAFN+ +Q  + S
Sbjct: 367 WCVVNNNKDLS--NASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHS 426

Query: 426 SSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI 481
           + +C F  L  +TTVDPS        D CRF+I +DTS  H  ++ P    + P +
Sbjct: 427 AESCNFGGLGLITTVDPS-------EDNCRFSIQLDTS--HSSSQTPNFFQSWPLL 467

BLAST of CmaCh04G021020 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 229.6 bits (584), Expect = 8.0e-59
Identity = 145/449 (32.29%), Postives = 244/449 (54.34%), Query Frame = 0

Query: 7   LFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGAL 66
           LFL   S L  + L+        N GT  ++   P  VV L+K    N+V+L++AD   L
Sbjct: 9   LFLFASSALSQDSLI------GVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSML 68

Query: 67  QALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVGNEPFLN 126
            A  ++G+QV++ +PN+ L  +S +   A  WV +NV+ +     T+I  +AVG+E   +
Sbjct: 69  LAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYP--ATNITTIAVGSEVLTS 128

Query: 127 AYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFRPDIRDL 186
             N + +  +  A++ +QAAL+ A L RQ+KV+ P ++ +   ++  PS   F      +
Sbjct: 129 LTNAASVLVS--ALKYIQAALVTANLDRQIKVSTPHSSTII-LDSFPPSQAFFNKTWDPV 188

Query: 187 MVNIIKFLSNNAAPLTINIYPFLS-LNADPHFPKEYAFFNGNAA--PVIDGS--ISYTNV 246
           +V ++KFL +  +PL +N+YP+   + ++   P +YA F    A    +D +  + YTNV
Sbjct: 189 IVPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNV 248

Query: 247 LDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKA---NAQRFNQGLVERINRRQ 306
            DA  D    A+    F+N+PI++ E GWP+ G PS + A   NA  +N  L++ +  + 
Sbjct: 249 FDAIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKT 308

Query: 307 GTPKRP-VPVDTYIFAIIDEDAKSIQPGSF-ERHWGVFNNDGTIKYSLNMGKGRSLVPAK 366
           GTPK P   V TYI+ + +ED +   PG   E++WG+F  +GT  Y+L +    +++   
Sbjct: 309 GTPKHPGTAVTTYIYELYNEDTR---PGPVSEKNWGLFYTNGTPVYTLRLAGAGAILAND 368

Query: 367 GVRYLPKEWCVMSPQASLSDPNLPNSVAYAC--DHADCTSLSYGSSCSQL-DAKSNVSYA 426
                 + +C+   +  +    L  ++ +AC     DC++L  G SC +  D  ++ +YA
Sbjct: 369 TTN---QTFCI--AKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYA 428

Query: 427 FNAYFQTMNQSSSACKFSNLATVTTVDPS 443
           FNAY+Q M ++S +C F  +ATVTT DPS
Sbjct: 429 FNAYYQKMGKASGSCDFKGVATVTTTDPS 438

BLAST of CmaCh04G021020 vs. ExPASy TrEMBL
Match: A0A6J1JRN7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111487729 PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.1e-295
Identity = 502/502 (100.00%), Postives = 502/502 (100.00%), Query Frame = 0

Query: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60
           MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE
Sbjct: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60

Query: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120
           ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG
Sbjct: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120

Query: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180
           NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR
Sbjct: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180

Query: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240
           PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN
Sbjct: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240

Query: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300
           VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT
Sbjct: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300

Query: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360
           PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY
Sbjct: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360

Query: 361 LPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQT 420
           LPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQT
Sbjct: 361 LPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQT 420

Query: 421 MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI 480
           MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI
Sbjct: 421 MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI 480

Query: 481 AHISCCHGITLLLALFVLILKY 503
           AHISCCHGITLLLALFVLILKY
Sbjct: 481 AHISCCHGITLLLALFVLILKY 502

BLAST of CmaCh04G021020 vs. ExPASy TrEMBL
Match: A0A6J1GQB3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111456119 PE=3 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 4.1e-292
Identity = 496/502 (98.80%), Postives = 499/502 (99.40%), Query Frame = 0

Query: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60
           MGHPCLLFLLI+SCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE
Sbjct: 1   MGHPCLLFLLIVSCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60

Query: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120
           ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG
Sbjct: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120

Query: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180
           NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR
Sbjct: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180

Query: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240
           PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN
Sbjct: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240

Query: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300
           VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT
Sbjct: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300

Query: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360
           PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY
Sbjct: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360

Query: 361 LPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQT 420
           LPK+WCVMSPQASLSDPNLPNSVAYACDHADCTSL+YGSSCSQLDAKSNVSYAFNAYFQT
Sbjct: 361 LPKQWCVMSPQASLSDPNLPNSVAYACDHADCTSLTYGSSCSQLDAKSNVSYAFNAYFQT 420

Query: 421 MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI 480
           MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKR RLARPPRTSS SP I
Sbjct: 421 MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRRRLARPPRTSSDSPRI 480

Query: 481 AHISCCHGITLLLALFVLILKY 503
           AHISCCHGITLLLALFVLILKY
Sbjct: 481 AHISCCHGITLLLALFVLILKY 502

BLAST of CmaCh04G021020 vs. ExPASy TrEMBL
Match: A0A5D3CT91 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00280 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 2.9e-253
Identity = 431/494 (87.25%), Postives = 462/494 (93.52%), Query Frame = 0

Query: 9   LLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGALQA 68
           LL++ CL     VKG  GFACNWGTRSSHPLPPDIVVKL+KDNGFNKVKLFEADPGALQA
Sbjct: 11  LLVVCCL---LFVKGGWGFACNWGTRSSHPLPPDIVVKLMKDNGFNKVKLFEADPGALQA 70

Query: 69  LGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVGNEPFLNAY 128
           LGNSGLQVMLGIPNEFLA L+S+VRVAE WVAKNVSYFVSNFGT+IRYVAVGNEPFLNAY
Sbjct: 71  LGNSGLQVMLGIPNEFLASLASSVRVAENWVAKNVSYFVSNFGTNIRYVAVGNEPFLNAY 130

Query: 129 NGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFRPDIRDLMV 188
           NGSFLQ+TFPA+QN+QAALIKAGLGRQVKVT+PLNADVYET NGLPSGGNFRPDIRDLMV
Sbjct: 131 NGSFLQSTFPALQNIQAALIKAGLGRQVKVTIPLNADVYETSNGLPSGGNFRPDIRDLMV 190

Query: 189 NIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTNVLDANFDT 248
           NIIKFL+NNA+PLTINIYPFLSLNADPHFPKEYAFF+GNAAPVIDGSISYTNVLDANFDT
Sbjct: 191 NIIKFLNNNASPLTINIYPFLSLNADPHFPKEYAFFSGNAAPVIDGSISYTNVLDANFDT 250

Query: 249 LVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGTPKRPVPVD 308
           LVSALEKNGFS+MP+IIGEVGWPTDGDP+ANKANAQRFNQGL+ERINRRQGTPKRPVP D
Sbjct: 251 LVSALEKNGFSSMPLIIGEVGWPTDGDPNANKANAQRFNQGLIERINRRQGTPKRPVPTD 310

Query: 309 TYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRYLPKEWCVM 368
           TYIFAIIDEDAKSIQPGSFERHWGVFN DGTIKYSLNMG G+ LVPAKGV+YL K+WCVM
Sbjct: 311 TYIFAIIDEDAKSIQPGSFERHWGVFNYDGTIKYSLNMGNGKQLVPAKGVQYLSKQWCVM 370

Query: 369 SPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQTMNQSSSAC 428
           SPQASLSDPN+PNSVAYACDHADCTSLSYGSSCS LDAKSNVSYAFNAYFQTMNQSS+AC
Sbjct: 371 SPQASLSDPNIPNSVAYACDHADCTSLSYGSSCSNLDAKSNVSYAFNAYFQTMNQSSNAC 430

Query: 429 KFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASP-TIAHISCCH 488
           KFSNLAT++TVDPSPPNQGN RDGCRF IMIDT+K+ RLARPPRTSS +   I   SC +
Sbjct: 431 KFSNLATISTVDPSPPNQGNNRDGCRFGIMIDTTKKRRLARPPRTSSGTRLRIGQKSCWY 490

Query: 489 GITLLLALFVLILK 502
            I+ LL L +LI+K
Sbjct: 491 WISFLLVLSILIMK 501

BLAST of CmaCh04G021020 vs. ExPASy TrEMBL
Match: A0A0A0KPC5 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G561790 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 6.4e-253
Identity = 430/504 (85.32%), Postives = 463/504 (91.87%), Query Frame = 0

Query: 1   MGHPCL--LFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKL 60
           MGHP L    LL++ CL    LVK   GFACNWGTRSSHPLPPDIVVKL+KDNGFNKVKL
Sbjct: 1   MGHPKLWGSLLLVVCCL---SLVKSGFGFACNWGTRSSHPLPPDIVVKLMKDNGFNKVKL 60

Query: 61  FEADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVA 120
           FEADPGALQALGNSGLQVMLGIPNEFLAPL+S+VRVAE WVAKNVSYF+SNFGT+IRYVA
Sbjct: 61  FEADPGALQALGNSGLQVMLGIPNEFLAPLASSVRVAENWVAKNVSYFISNFGTNIRYVA 120

Query: 121 VGNEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGN 180
           VGNEPFLNAYNGSFLQ+TFPA+QN+QAALIKAGLGRQVKVT+PLNADVYET NGLPSGGN
Sbjct: 121 VGNEPFLNAYNGSFLQSTFPALQNIQAALIKAGLGRQVKVTIPLNADVYETSNGLPSGGN 180

Query: 181 FRPDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISY 240
           FRPDIRDLMVNIIKFLSNNA PLTINIYPFLSLNADPHFPKEYAFF+GNAAPVIDGSISY
Sbjct: 181 FRPDIRDLMVNIIKFLSNNACPLTINIYPFLSLNADPHFPKEYAFFSGNAAPVIDGSISY 240

Query: 241 TNVLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQ 300
           TNVLDANFDTLVSALEKNGF++MP+IIGEVGWPTDGDPSANKANAQRFNQGL+ERINRRQ
Sbjct: 241 TNVLDANFDTLVSALEKNGFTSMPLIIGEVGWPTDGDPSANKANAQRFNQGLIERINRRQ 300

Query: 301 GTPKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGV 360
           GTPKRPVP DTYIFAI+DEDAKSIQPG+FERHWGVFN DGTIKYSLNMG G+ LVPAKGV
Sbjct: 301 GTPKRPVPTDTYIFAIVDEDAKSIQPGNFERHWGVFNYDGTIKYSLNMGNGKQLVPAKGV 360

Query: 361 RYLPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYF 420
           +YL K+WCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSC  LDAKSNVSYAFNAYF
Sbjct: 361 QYLSKQWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCGNLDAKSNVSYAFNAYF 420

Query: 421 QTMNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASP 480
           QTMNQSS+ACKFSNLAT++T+DPSPPNQGN RD C+F IMIDT+K+  LARPPRTSS + 
Sbjct: 421 QTMNQSSNACKFSNLATISTIDPSPPNQGNNRDACQFGIMIDTTKKRHLARPPRTSSGTK 480

Query: 481 -TIAHISCCHGITLLLALFVLILK 502
             I   SC + I+ LL   +LI+K
Sbjct: 481 LRIGQNSCWYWISFLLVPSILIMK 501

BLAST of CmaCh04G021020 vs. ExPASy TrEMBL
Match: E5GB65 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.2e-252
Identity = 429/494 (86.84%), Postives = 460/494 (93.12%), Query Frame = 0

Query: 9   LLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGALQA 68
           LL++ CL     VKG  GFACNWGTRSSHPLPPDIVVKL+KDNGFNKVKLFEADPGALQA
Sbjct: 11  LLVVCCL---LFVKGGWGFACNWGTRSSHPLPPDIVVKLMKDNGFNKVKLFEADPGALQA 70

Query: 69  LGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVGNEPFLNAY 128
           LGNSGLQVMLGIPNEFLA L+S+VRVAE WVAKNVSYFVSNFGT+IRYVAVGNEPFLNAY
Sbjct: 71  LGNSGLQVMLGIPNEFLASLASSVRVAENWVAKNVSYFVSNFGTNIRYVAVGNEPFLNAY 130

Query: 129 NGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFRPDIRDLMV 188
           NGSFLQ+TFPA+QN+QAALIKAGLGRQVKVT+PLNADVYET NGLPSGGNFRPDIRDLMV
Sbjct: 131 NGSFLQSTFPALQNIQAALIKAGLGRQVKVTIPLNADVYETSNGLPSGGNFRPDIRDLMV 190

Query: 189 NIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTNVLDANFDT 248
           NIIKFL+NNA+PLTINIYPFLSLNADPHFPKEYAFF+GNAAPVIDGSISYTNVLDANFDT
Sbjct: 191 NIIKFLNNNASPLTINIYPFLSLNADPHFPKEYAFFSGNAAPVIDGSISYTNVLDANFDT 250

Query: 249 LVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGTPKRPVPVD 308
           LVSALEKNGFS+MP+IIGEVGWPTDGDP+ANKANAQRFNQGL+ERINRRQGTPKRPVP D
Sbjct: 251 LVSALEKNGFSSMPLIIGEVGWPTDGDPNANKANAQRFNQGLIERINRRQGTPKRPVPTD 310

Query: 309 TYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRYLPKEWCVM 368
           TYIFAIIDEDAKSIQPGSFERHWGVFN DGTIKYSLNMG G+ LVPAKGV+YL K+WCVM
Sbjct: 311 TYIFAIIDEDAKSIQPGSFERHWGVFNYDGTIKYSLNMGNGKQLVPAKGVQYLSKQWCVM 370

Query: 369 SPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQTMNQSSSAC 428
           SPQASLSDPN+PNSVAYACDHADCTSLSYGSSC  LDAKSNVSYAFNAYFQTMNQS +AC
Sbjct: 371 SPQASLSDPNIPNSVAYACDHADCTSLSYGSSCGNLDAKSNVSYAFNAYFQTMNQSPNAC 430

Query: 429 KFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASP-TIAHISCCH 488
           KFSNLAT++TVDPSPPNQGN RDGCRF IMIDT+K+ RLARPPRTSS +   I   SC +
Sbjct: 431 KFSNLATISTVDPSPPNQGNNRDGCRFGIMIDTTKKRRLARPPRTSSGTRLRIGQKSCWY 490

Query: 489 GITLLLALFVLILK 502
            I+ LL L +LI+K
Sbjct: 491 WISFLLVLSILIMK 501

BLAST of CmaCh04G021020 vs. NCBI nr
Match: XP_022991010.1 (glucan endo-1,3-beta-glucosidase 5-like [Cucurbita maxima])

HSP 1 Score: 1023.5 bits (2645), Expect = 6.3e-295
Identity = 502/502 (100.00%), Postives = 502/502 (100.00%), Query Frame = 0

Query: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60
           MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE
Sbjct: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60

Query: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120
           ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG
Sbjct: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120

Query: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180
           NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR
Sbjct: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180

Query: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240
           PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN
Sbjct: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240

Query: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300
           VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT
Sbjct: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300

Query: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360
           PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY
Sbjct: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360

Query: 361 LPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQT 420
           LPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQT
Sbjct: 361 LPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQT 420

Query: 421 MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI 480
           MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI
Sbjct: 421 MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI 480

Query: 481 AHISCCHGITLLLALFVLILKY 503
           AHISCCHGITLLLALFVLILKY
Sbjct: 481 AHISCCHGITLLLALFVLILKY 502

BLAST of CmaCh04G021020 vs. NCBI nr
Match: XP_022953659.1 (glucan endo-1,3-beta-glucosidase 5-like [Cucurbita moschata])

HSP 1 Score: 1013.1 bits (2618), Expect = 8.5e-292
Identity = 496/502 (98.80%), Postives = 499/502 (99.40%), Query Frame = 0

Query: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60
           MGHPCLLFLLI+SCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE
Sbjct: 1   MGHPCLLFLLIVSCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60

Query: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120
           ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG
Sbjct: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120

Query: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180
           NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR
Sbjct: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180

Query: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240
           PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN
Sbjct: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240

Query: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300
           VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT
Sbjct: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300

Query: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360
           PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY
Sbjct: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360

Query: 361 LPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQT 420
           LPK+WCVMSPQASLSDPNLPNSVAYACDHADCTSL+YGSSCSQLDAKSNVSYAFNAYFQT
Sbjct: 361 LPKQWCVMSPQASLSDPNLPNSVAYACDHADCTSLTYGSSCSQLDAKSNVSYAFNAYFQT 420

Query: 421 MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI 480
           MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKR RLARPPRTSS SP I
Sbjct: 421 MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRRRLARPPRTSSDSPRI 480

Query: 481 AHISCCHGITLLLALFVLILKY 503
           AHISCCHGITLLLALFVLILKY
Sbjct: 481 AHISCCHGITLLLALFVLILKY 502

BLAST of CmaCh04G021020 vs. NCBI nr
Match: KAG6601858.1 (Glucan endo-1,3-beta-glucosidase 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032557.1 Glucan endo-1,3-beta-glucosidase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1010.0 bits (2610), Expect = 7.2e-291
Identity = 494/502 (98.41%), Postives = 498/502 (99.20%), Query Frame = 0

Query: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60
           MGHPCLLFLLI+SCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE
Sbjct: 1   MGHPCLLFLLIVSCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60

Query: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120
           ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG
Sbjct: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120

Query: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180
           NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR
Sbjct: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180

Query: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240
           PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN
Sbjct: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240

Query: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300
           VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT
Sbjct: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300

Query: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360
           PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY
Sbjct: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRY 360

Query: 361 LPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQT 420
           LPK+WCVMSPQASLSDPNLPNSVAYACDHADCTSL+YGSSCSQLDAKSNVSYAFNAYFQT
Sbjct: 361 LPKQWCVMSPQASLSDPNLPNSVAYACDHADCTSLTYGSSCSQLDAKSNVSYAFNAYFQT 420

Query: 421 MNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTI 480
           MNQSSSACKFSNLATVTTVDP+PPNQGNTRD CRFAIMIDTSKR RLARPPRTSSA P I
Sbjct: 421 MNQSSSACKFSNLATVTTVDPTPPNQGNTRDSCRFAIMIDTSKRRRLARPPRTSSAPPRI 480

Query: 481 AHISCCHGITLLLALFVLILKY 503
           AHISCCHGITLLLALFVLILKY
Sbjct: 481 AHISCCHGITLLLALFVLILKY 502

BLAST of CmaCh04G021020 vs. NCBI nr
Match: XP_038906946.1 (glucan endo-1,3-beta-glucosidase 5-like [Benincasa hispida])

HSP 1 Score: 890.2 bits (2299), Expect = 8.3e-255
Identity = 440/507 (86.79%), Postives = 469/507 (92.50%), Query Frame = 0

Query: 1   MGH---PCLLFLLIISCLGH-EGL-VKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNK 60
           MGH     LL  L++ CLG+ EGL VKG LGFACNWGTRSSHPLPPDIVVKL+KDNGF+K
Sbjct: 1   MGHRKWGSLLLALVVCCLGYEEGLFVKGGLGFACNWGTRSSHPLPPDIVVKLMKDNGFSK 60

Query: 61  VKLFEADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIR 120
           VKLFEADPGALQALGNSGLQVMLGIPNEFLAPL+S+VRVAE WV+KNVSYFVSNFGTDIR
Sbjct: 61  VKLFEADPGALQALGNSGLQVMLGIPNEFLAPLASSVRVAENWVSKNVSYFVSNFGTDIR 120

Query: 121 YVAVGNEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPS 180
           YVAVGNEPFLNAYNGSFLQTTFPA+QN+QAALIKAGLGRQVKVT+PLNADVYET NGLPS
Sbjct: 121 YVAVGNEPFLNAYNGSFLQTTFPALQNIQAALIKAGLGRQVKVTIPLNADVYETGNGLPS 180

Query: 181 GGNFRPDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGS 240
           GGNFRPDIRDLMVNIIKFLSNNA+PLTINIYPFLSLNADPHFPKEYAFF+GNAAPVIDGS
Sbjct: 181 GGNFRPDIRDLMVNIIKFLSNNASPLTINIYPFLSLNADPHFPKEYAFFSGNAAPVIDGS 240

Query: 241 ISYTNVLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERIN 300
           ISYTNVLDANFDTLVSALEKNGFS+MP+IIGEVGWPTDGDP+AN ANAQRFNQGL+ERIN
Sbjct: 241 ISYTNVLDANFDTLVSALEKNGFSSMPLIIGEVGWPTDGDPNANNANAQRFNQGLIERIN 300

Query: 301 RRQGTPKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPA 360
           RRQGTPKRPVP DTYIFAIIDEDAKSIQPGSFERHWG+FN DGTIK SLNMG  + LVPA
Sbjct: 301 RRQGTPKRPVPTDTYIFAIIDEDAKSIQPGSFERHWGIFNYDGTIKCSLNMGNRKPLVPA 360

Query: 361 KGVRYLPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFN 420
           KGV+YL K+WCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSC  LDA+SNVSYAFN
Sbjct: 361 KGVQYLSKQWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCGNLDARSNVSYAFN 420

Query: 421 AYFQTMNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSS 480
           AYFQTMNQSS+ACKFSNLAT++TVDPSP NQGN RDGCRF IMIDT+K+  LARPPRTSS
Sbjct: 421 AYFQTMNQSSNACKFSNLATISTVDPSPSNQGNGRDGCRFGIMIDTTKKRHLARPPRTSS 480

Query: 481 ASP-TIAHISCCHGITLLLALFVLILK 502
           AS   I  IS CH I  LLAL +LI+K
Sbjct: 481 ASTLRIGQISYCHWIGFLLALSILIMK 507

BLAST of CmaCh04G021020 vs. NCBI nr
Match: KAA0046409.1 (glucan endo-1,3-beta-glucosidase 6 [Cucumis melo var. makuwa] >TYK14652.1 glucan endo-1,3-beta-glucosidase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 884.0 bits (2283), Expect = 6.0e-253
Identity = 431/494 (87.25%), Postives = 462/494 (93.52%), Query Frame = 0

Query: 9   LLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGALQA 68
           LL++ CL     VKG  GFACNWGTRSSHPLPPDIVVKL+KDNGFNKVKLFEADPGALQA
Sbjct: 11  LLVVCCL---LFVKGGWGFACNWGTRSSHPLPPDIVVKLMKDNGFNKVKLFEADPGALQA 70

Query: 69  LGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVGNEPFLNAY 128
           LGNSGLQVMLGIPNEFLA L+S+VRVAE WVAKNVSYFVSNFGT+IRYVAVGNEPFLNAY
Sbjct: 71  LGNSGLQVMLGIPNEFLASLASSVRVAENWVAKNVSYFVSNFGTNIRYVAVGNEPFLNAY 130

Query: 129 NGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFRPDIRDLMV 188
           NGSFLQ+TFPA+QN+QAALIKAGLGRQVKVT+PLNADVYET NGLPSGGNFRPDIRDLMV
Sbjct: 131 NGSFLQSTFPALQNIQAALIKAGLGRQVKVTIPLNADVYETSNGLPSGGNFRPDIRDLMV 190

Query: 189 NIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTNVLDANFDT 248
           NIIKFL+NNA+PLTINIYPFLSLNADPHFPKEYAFF+GNAAPVIDGSISYTNVLDANFDT
Sbjct: 191 NIIKFLNNNASPLTINIYPFLSLNADPHFPKEYAFFSGNAAPVIDGSISYTNVLDANFDT 250

Query: 249 LVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGTPKRPVPVD 308
           LVSALEKNGFS+MP+IIGEVGWPTDGDP+ANKANAQRFNQGL+ERINRRQGTPKRPVP D
Sbjct: 251 LVSALEKNGFSSMPLIIGEVGWPTDGDPNANKANAQRFNQGLIERINRRQGTPKRPVPTD 310

Query: 309 TYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRYLPKEWCVM 368
           TYIFAIIDEDAKSIQPGSFERHWGVFN DGTIKYSLNMG G+ LVPAKGV+YL K+WCVM
Sbjct: 311 TYIFAIIDEDAKSIQPGSFERHWGVFNYDGTIKYSLNMGNGKQLVPAKGVQYLSKQWCVM 370

Query: 369 SPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQTMNQSSSAC 428
           SPQASLSDPN+PNSVAYACDHADCTSLSYGSSCS LDAKSNVSYAFNAYFQTMNQSS+AC
Sbjct: 371 SPQASLSDPNIPNSVAYACDHADCTSLSYGSSCSNLDAKSNVSYAFNAYFQTMNQSSNAC 430

Query: 429 KFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSASP-TIAHISCCH 488
           KFSNLAT++TVDPSPPNQGN RDGCRF IMIDT+K+ RLARPPRTSS +   I   SC +
Sbjct: 431 KFSNLATISTVDPSPPNQGNNRDGCRFGIMIDTTKKRRLARPPRTSSGTRLRIGQKSCWY 490

Query: 489 GITLLLALFVLILK 502
            I+ LL L +LI+K
Sbjct: 491 WISFLLVLSILIMK 501

BLAST of CmaCh04G021020 vs. TAIR 10
Match: AT5G20870.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 611.7 bits (1576), Expect = 5.3e-175
Identity = 297/479 (62.00%), Postives = 368/479 (76.83%), Query Frame = 0

Query: 7   LFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGAL 66
           L L  +S    + ++K   G ACNWGT++SHPLPP+IVVKL++DNGFNKVKLFEADPGAL
Sbjct: 10  LILFCLSIFFLQNILKNVEGLACNWGTQASHPLPPNIVVKLLRDNGFNKVKLFEADPGAL 69

Query: 67  QALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVGNEPFLN 126
           +ALG SG+QVM+GIPN+ LA ++STV  AE WV +NVS ++S +GTDIRYVAVGNEPFL 
Sbjct: 70  RALGKSGIQVMVGIPNDLLATMASTVTNAELWVQQNVSQYISRYGTDIRYVAVGNEPFLK 129

Query: 127 AYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFRPDIRDL 186
            YN  F+++T+PA+QNVQAAL+KAGLGRQVKVTVPLNADVYE+ +GLPS G+FR DI+ L
Sbjct: 130 TYNNRFVRSTYPALQNVQAALVKAGLGRQVKVTVPLNADVYESSDGLPSSGDFRSDIKTL 189

Query: 187 MVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFF-----NGNAAPVIDGSISYTNV 246
           M++I++FL+++ +P+T NIYPFLSLNADP+FP+EYAFF      G A PV+DGSISYTNV
Sbjct: 190 MISIVRFLADSVSPITFNIYPFLSLNADPNFPREYAFFPNGGGGGGAKPVVDGSISYTNV 249

Query: 247 LDANFDTLVSALEKNGF--SNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQG 306
            DANFDTLVSALEKNGF  + + II+GEVGWPTDGD +AN A AQRFNQGL+ RI + QG
Sbjct: 250 FDANFDTLVSALEKNGFDANKIEIIVGEVGWPTDGDQNANPAMAQRFNQGLLNRILQGQG 309

Query: 307 TPKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVR 366
           TP+R +  + YIF+++DEDAKSI PG FERHWG+F+ DG +KY L++G GR LVP KGVR
Sbjct: 310 TPRRRMAPEVYIFSLVDEDAKSIDPGKFERHWGIFSYDGAVKYPLSLGNGRPLVPTKGVR 369

Query: 367 YLPKEWCVMSPQASLSD-PNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYF 426
           Y  +EWCV+S QA+ +       S  YAC +ADCTSL  GSSC+ LD  +N SYAFN YF
Sbjct: 370 YQAREWCVLSTQAAGNGAATWQASATYACQNADCTSLGPGSSCAALDPTANASYAFNMYF 429

Query: 427 QTMNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTSKRHRLARPPRTSSAS 478
           Q M+    +C F+NL  VT +DPS          CRF I IDTS++     PPR S AS
Sbjct: 430 QKMDHRRGSCDFNNLGVVTKIDPS-------SGSCRFPIEIDTSRQQMSNPPPRNSGAS 481

BLAST of CmaCh04G021020 vs. TAIR 10
Match: AT4G31140.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 561.6 bits (1446), Expect = 6.3e-160
Identity = 267/466 (57.30%), Postives = 340/466 (72.96%), Query Frame = 0

Query: 26  GFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGALQALGNSGLQVMLGIPNEFL 85
           G   NWG+++ HPLPP  VV+L+++NG  KVKLFEAD   L+AL  +G+QVM+GIPN+ L
Sbjct: 26  GIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKALSRTGIQVMVGIPNDLL 85

Query: 86  APLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVGNEPFLNAYNGSFLQTTFPAMQNVQA 145
           APL+ +V  AE WV++NVS  VS+ G DIRYVAVGNEPFL A+NG+F   T PA+QN+Q+
Sbjct: 86  APLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGTFEGITLPALQNIQS 145

Query: 146 ALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFRPDIRDLMVNIIKFLSNNAAPLTINI 205
           A+IKAGL  QVKVTVPLNADVY++ + LPS G+FRP+IRDLM+NI+KFLS+N AP TINI
Sbjct: 146 AIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLMLNIVKFLSDNGAPFTINI 205

Query: 206 YPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTNVLDANFDTLVSALEKNGFSNMPIII 265
           YPF+SL  DP+FP E+AFF+G   P+ D    Y NVLDAN+DTLV +L+KNGF N+ II+
Sbjct: 206 YPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYDTLVWSLQKNGFGNLTIIV 265

Query: 266 GEVGWPTDGDPSANKANAQRFNQGLVERINRRQGTPKRPVPVDTYIFAIIDEDAKSIQPG 325
           GEVGWPTDGD +AN   A+R+NQG + R    +GTP RP  +D Y+F +IDEDAKSIQPG
Sbjct: 266 GEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAMDAYLFGLIDEDAKSIQPG 325

Query: 326 SFERHWGVFNNDGTIKYSLNMGKGRSLVPAKGVRYLPKEWCVMSPQASLSDPNLPNSVAY 385
           +FERHWG+F  DG  KY L++G G  L+PAK V YL K+WC+++P A+L DP L  SV+Y
Sbjct: 326 NFERHWGIFYIDGQPKYQLSLGSGNGLIPAKDVHYLAKKWCILAPNANLQDPQLGPSVSY 385

Query: 386 ACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQTMNQSSSACKFSNLATVTTVDPSPPN 445
           ACDHADCTSL YGSSC  L+   NVSYAFN+Y+Q  NQ  SACKF  L+ V+T DPS   
Sbjct: 386 ACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSACKFPGLSIVSTRDPSV-- 445

Query: 446 QGNTRDGCRFAIMIDTSKRHRLARPPRTSSASPTIAHISCCHGITL 492
                  C+F IMI +      +     + ++  +  +S C  I L
Sbjct: 446 -----GSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSICLYIVL 484

BLAST of CmaCh04G021020 vs. TAIR 10
Match: AT5G58090.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 558.9 bits (1439), Expect = 4.1e-159
Identity = 264/462 (57.14%), Postives = 342/462 (74.03%), Query Frame = 0

Query: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60
           MG   +L LL ++      L + A     NWGT++SHPLPPDIVV+++++NG  KVKLF+
Sbjct: 1   MGWGSVLLLLAVAL-----LCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFD 60

Query: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120
           A+   L+ALG SG++VM+GIPNE LA L+S+++ AE+WVAKNVS  +S    +IRYVAVG
Sbjct: 61  AEYDTLRALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVG 120

Query: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYETENGLPSGGNFR 180
           NEPFL+ YNGS+L TTFPA++N+Q A+IKAGL  QVKVT PLNADVY++    PSGG+FR
Sbjct: 121 NEPFLSTYNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFR 180

Query: 181 PDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTN 240
            +IRDLM+ I+KFLS N  P T+NIYP++SL  +P FP +YAFF+GNA P+ DG   Y N
Sbjct: 181 ANIRDLMITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYN 240

Query: 241 VLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGT 300
           + DAN+DTLV ALEKNGF NMPIIIGE+GWPTDGD +AN   A++FNQG +  I+  +GT
Sbjct: 241 MFDANYDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGT 300

Query: 301 PKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNMGKGR--SLVPAKGV 360
           P+RP P+D Y+F++IDEDAKS+QPG FERHWG+F  DG  KY+LN+G     +L+ AKGV
Sbjct: 301 PRRPGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNLGTTNTGALIQAKGV 360

Query: 361 RYLPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYF 420
           RYL ++WCVM P   L DP +  +V+YAC   DCTSL  G+SC+ LD K N+SYAFN+Y+
Sbjct: 361 RYLERKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYY 420

Query: 421 QTMNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMID 461
           Q  +Q  +ACKF N++ VT  DPS          CRF IMI+
Sbjct: 421 QIQDQLDTACKFPNISEVTKTDPSTGT-------CRFPIMIE 450

BLAST of CmaCh04G021020 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 468.0 bits (1203), Expect = 9.5e-132
Identity = 237/442 (53.62%), Postives = 301/442 (68.10%), Query Frame = 0

Query: 26  GFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFEADPGALQALGNSGLQVMLGIPNEFL 85
           G   NWGT ++H LPP  VV+++KDN  NKVKLF+AD   + AL  SGL+VM+ IPN+ L
Sbjct: 20  GLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPNDQL 79

Query: 86  APLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVGNEPFLNAYNGSFLQTTFPAMQNVQA 145
             + S  R A++WV KNV+ +  N G +I +VAVGNEPFL +YNGSF+  TFPA+QN+Q 
Sbjct: 80  KVMGSYDR-AKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQN 139

Query: 146 ALIKAGLGRQVKVTVPLNADVYE--TENGLPSGGNFRPDIRDLMVNIIKFLSNNAAPLTI 205
           AL +AGLG  VK TVPLNADVY+  + N +PS G FRPDI   M  I+ FL NN+AP+TI
Sbjct: 140 ALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSAPITI 199

Query: 206 NIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISYTNVLDANFDTLVSALEKNGFSNMPI 265
           NIYPFLSL  +  FP  YAFF+G A PV D  I+YTNV DANFDTLVSAL+  G  +MPI
Sbjct: 200 NIYPFLSLYGNDDFPLNYAFFDG-AKPVDDNGIAYTNVFDANFDTLVSALKAVGHGDMPI 259

Query: 266 IIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQGTPKRPVPVDTYIFAIIDEDAKSIQ 325
           I+GEVGWPT+GD  AN  +A RF  GL+ R+   +GTP RP  ++ Y+F ++DEDAKSI 
Sbjct: 260 IVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTYIEVYLFGLLDEDAKSIA 319

Query: 326 PGSFERHWGVFNNDGTIKYSLNM---GKGRSLVPAKGVRYLPKEWCVMSPQASLSDPNLP 385
           PG FERHWG+F  DG  K+ +++   G+ + L+ A+ V Y PK+WC+ + +A      L 
Sbjct: 320 PGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAK-DLTKLA 379

Query: 386 NSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFNAYFQTMNQSSSACKFSNLATVTTVD 445
            ++ YAC  +DCT+L YGSSC+ LDA  N SYAFN YFQ  NQ   AC F  LAT+TT +
Sbjct: 380 ANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLATITTKN 439

Query: 446 PSPPNQGNTRDGCRFAIMIDTS 463
            S   QG     C F I I  S
Sbjct: 440 IS---QGQ----CNFPIQIVAS 451

BLAST of CmaCh04G021020 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 466.1 bits (1198), Expect = 3.6e-131
Identity = 237/467 (50.75%), Postives = 310/467 (66.38%), Query Frame = 0

Query: 1   MGHPCLLFLLIISCLGHEGLVKGALGFACNWGTRSSHPLPPDIVVKLIKDNGFNKVKLFE 60
           M +   L +  +  +GH G++   LG   NWGT ++H LPP  VV+++KDN  NKVKLF+
Sbjct: 1   MSNLLALVVGFVIVIGHLGILVNGLG--VNWGTMATHKLPPKTVVQMLKDNNINKVKLFD 60

Query: 61  ADPGALQALGNSGLQVMLGIPNEFLAPLSSTVRVAEEWVAKNVSYFVSNFGTDIRYVAVG 120
           AD   + AL  SGL+VM+ IPN+ L  ++S  R A++WV KNV+ +  + G +I +VAVG
Sbjct: 61  ADETTMGALAGSGLEVMVAIPNDQLKVMTSYDR-AKDWVRKNVTRYNFDGGVNITFVAVG 120

Query: 121 NEPFLNAYNGSFLQTTFPAMQNVQAALIKAGLGRQVKVTVPLNADVYET--ENGLPSGGN 180
           NEPFL +YNGSF+  TFPA+ N+Q AL +AGLG  VK TVPLNADVY++   N +PS G 
Sbjct: 121 NEPFLKSYNGSFINLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGR 180

Query: 181 FRPDIRDLMVNIIKFLSNNAAPLTINIYPFLSLNADPHFPKEYAFFNGNAAPVIDGSISY 240
           FRPDI   M  I+ FL  N AP+TINIYPFLSL  +  FP  YAFF+G A P+ D  I Y
Sbjct: 181 FRPDIIGQMTQIVDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDG-AEPINDNGIDY 240

Query: 241 TNVLDANFDTLVSALEKNGFSNMPIIIGEVGWPTDGDPSANKANAQRFNQGLVERINRRQ 300
           TNV DANFDTLVS+L+  G  +MPII+GEVGWPT+GD  AN  +A RF  GL+ R+   +
Sbjct: 241 TNVFDANFDTLVSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNK 300

Query: 301 GTPKRPVPVDTYIFAIIDEDAKSIQPGSFERHWGVFNNDGTIKYSLNM---GKGRSLVPA 360
           GTP RP  ++ Y+F ++DEDAKSI PG FERHWG+F  DG  K+ +++   G+ + L+ A
Sbjct: 301 GTPLRPTYIEVYLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGA 360

Query: 361 KGVRYLPKEWCVMSPQASLSDPNLPNSVAYACDHADCTSLSYGSSCSQLDAKSNVSYAFN 420
           + V YLP +WC  +P+A      L  ++ YAC  +DCT+L YGSSC+ LDA  N SYAFN
Sbjct: 361 QNVPYLPNKWCTFNPEAK-DLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFN 420

Query: 421 AYFQTMNQSSSACKFSNLATVTTVDPSPPNQGNTRDGCRFAIMIDTS 463
            +FQ  NQ  SAC F  LAT+TT       Q  ++  C F I I  S
Sbjct: 421 MFFQVKNQDESACYFQGLATITT-------QNISQGQCNFPIQIVAS 455

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0888.9e-15957.30Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q93Z085.8e-15857.14Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q6NKW95.1e-13050.75Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q9FGH45.0e-10141.39Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
Q9ZU918.0e-5932.29Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JRN73.1e-295100.00(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111487729 PE=... [more]
A0A6J1GQB34.1e-29298.80(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111456119 P... [more]
A0A5D3CT912.9e-25387.25(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A0A0KPC56.4e-25385.32(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G561790 PE=3... [more]
E5GB653.2e-25286.84(1->3)-beta-glucan endohydrolase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022991010.16.3e-295100.00glucan endo-1,3-beta-glucosidase 5-like [Cucurbita maxima][more]
XP_022953659.18.5e-29298.80glucan endo-1,3-beta-glucosidase 5-like [Cucurbita moschata][more]
KAG6601858.17.2e-29198.41Glucan endo-1,3-beta-glucosidase 5, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_038906946.18.3e-25586.79glucan endo-1,3-beta-glucosidase 5-like [Benincasa hispida][more]
KAA0046409.16.0e-25387.25glucan endo-1,3-beta-glucosidase 6 [Cucumis melo var. makuwa] >TYK14652.1 glucan... [more]
Match NameE-valueIdentityDescription
AT5G20870.15.3e-17562.00O-Glycosyl hydrolases family 17 protein [more]
AT4G31140.16.3e-16057.30O-Glycosyl hydrolases family 17 protein [more]
AT5G58090.14.1e-15957.14O-Glycosyl hydrolases family 17 protein [more]
AT2G19440.19.5e-13253.62O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.13.6e-13150.75O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 364..455
e-value: 2.6E-30
score: 116.7
IPR012946X8 domainPFAMPF07983X8coord: 365..435
e-value: 3.2E-15
score: 56.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 27..351
e-value: 4.1E-108
score: 363.1
NoneNo IPR availableGENE3D1.20.58.1040coord: 362..464
e-value: 2.0E-9
score: 39.7
NoneNo IPR availablePANTHERPTHR32227:SF59O-GLYCOSYL HYDROLASES FAMILY 17 PROTEINcoord: 7..474
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 28..345
e-value: 3.2E-67
score: 227.4
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 261..274
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 7..474
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 30..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G021020.1CmaCh04G021020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds