CmaCh04G020800 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G020800
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiontwo-component response regulator ORR26-like
LocationCma_Chr04: 14160800 .. 14163272 (-)
RNA-Seq ExpressionCmaCh04G020800
SyntenyCmaCh04G020800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGAAAATGGGTCATTGGGTCCCTGATTCTCTTCCTAGCTTTGCCAAAAATCTTCATATCTTGGTGGTGGATCATGAAACCTTATCTCTCAACCACTTGAGTTCCTTGCTTAGGGAGCAGTCTTACCATGGTACATAGCATCACAATATTTCCATTAATCTCTTTCACCATCCTAATTTAATTTAATGGTCTAAAGCTAATTTTATGCTTCTGTTTGTATCGGATAACATCCTAATTTTAATTTTGTCAGTAATGATCTAAAGCTAAATTTTATGCCAATTGTTTGTATCTGATAACATGTAAAGTTTATTTCTCAAACAGAAGAAAAAAAATTGGGAAATGTTAACTTGATTTTTTAATTATTTTATTGATTTTTTATTCATTTGAAAGGAAGTTCATGAGTGATTGTTTTTATTAATACTATATTTCCATATAAAGATATTTGATGAGTTGCTCAATTTCTTCTTTTTCTGGGAATTAAATTCAAAGCATAAAAGTATAAGATAGGAATGTCTTCCTTGTTTAAGTTTCTACTCGACCAATTTTGTGTATGTTGGTTCATTTATGGGACTAATTACATGAGTATCTTCAACTTTAAGTTTTTGTTTTTCCTTTTCTATGAACGAAACGTGCATGACTTGATTTTTTTTTTTTTTTTTAATTTTCAGTTACGACCACTGTATTGGGATCTATTGCTCTATCAATGATTCAAGAAAAAAGTGATGAGTTCGACCTTGTCATGGCTAACGTTAGCATGCCCGACATGGACAGTTTTTCATTCTTGCACGTGCTCCTTAAGATGAAAATTGTTGTCATTTGTAAGCTCAATTTTTCTCTACAAATCTCATTCAATTAAATGTCTTCATTATTCTCAAGCTATTTTGTTGTTGGGCAGTTATGTCATCAGTGATGAACTTGGACGTGGCTACGAAAGCATTGGCTGAAGGAGCGTGTTATTTTCTTCAGAAACCTATTTCCAAGGATGATCTCAAATATGTATGGCAACACGTGTATCGAAGGAACAGAAACATATCAAAGCTAAACCATGAAGCAAATTGCGTAGAAAAAGCCAAATATGGAAAAGAATCTGTCGGCATCCAAAACGACGACACTGTTGTTTTGTCTCAGCCCACCGATGAAGTTAGTATTAATAATAACGGTAGTATTAACTATCAACTTATGAGCTATAAAGAAGAAGGGCAGAATCAACCAACTAATTCCCATGATACCCTAGTTGCTTCATACTTCGAAGGAAAGAGATTAATGGATGACATAGAAGGAACAAGCAAGGAGAAGAGAGTTATATATTACTCAACGCCTACCAATTTTGGAGATACAAGAATTGATGAGGATAATAGAAGAATGAAGGAATATTATATTTCTAGTGATAACAGATCACGTGTCGTTTGGAATGTGGAACGACGTCGCAAATTCTCCGACGCTCTCAACAAGCTAGGAGATAAATGTAATTTCGCCTCTCTTTCCAGAGTTATTTTTATTTTTTTAGTATTTAAATTCAATAATGCATTTTTAATTGTTTTACTTACATTTTCTTCTTGTTTTGAAGGTCGTCCAAAACTTATACTGAAATTGATGAATGAACCATCCTTGACACTGCGCCAGGTAGCTAACCACCTGCAGGTATTGTTCATTCTTGTTCGCATAAAATAGAATTTGGTATTAGATTTGTTCTAACCATGCATTATCCTATCAGAAATACAAAGCACAAGTAGAGTCCATGAAAAAGGGGAGAGAAAATAAATTAGCTCCAAGGAGGGAAGCTTCCAAATTCAATTTCTCAGTCAGGACTCAACTTCCTCTTCCGTTGGTACCAAAGCACCCTCACGATGAGGCAAACAGCTCTACAAAGGGAAGTTTAACCTCTTTTATAGGAGGGGAAAGATTCCGACTTATTGCTCCTAAACCTGTGCCTAGTCCTAAACTGCCTGTTAGCGCCAACTTTGCAAATCATGGTCTCATAATGCTTGGCCAAAATTTTCAACCTGTTGATTCAAACTACAATTCAGTTCCATATATCATCAATCAGACAACTCCAAAGGTAACTTCTTCTTGTGTATTTGATGAAACTCAGTTCTCAGATAATCATCTTGATGTAGTACAGCAAGATTTGACTACTTTCAAGATTGAAAACCAAGAAGCAATGGGATGTAATGGCACTTCAGAAGGGATTCAGTTGATGTCAAACAACATGGCTTTTCCTGATTCAACCAACTTGGACAATATTGCTGCAAATTTTGGAAATGAAGGAAGCCAACAACAATCAGCCGAATTTGATTATCTCTTAAATTTTTTAGAAGATGATCCACACGAGTTTGACAGTGACTTAAATCTGAGTGACGTAGATAAGTACAGCGAGTGGCTAAAGAACACTGTTCTTGAAAATAGAAGCGGTCCAGACAGCTTTCTTGGTGACAGTGCAGAAAACTTTTCAACTGAAAACACCCAATAA

mRNA sequence

ATGCAGAAAATGGGTCATTGGGTCCCTGATTCTCTTCCTAGCTTTGCCAAAAATCTTCATATCTTGGTGGTGGATCATGAAACCTTATCTCTCAACCACTTGAGTTCCTTGCTTAGGGAGCAGTCTTACCATGTTACGACCACTGTATTGGGATCTATTGCTCTATCAATGATTCAAGAAAAAAGTGATGAGTTCGACCTTGTCATGGCTAACGTTAGCATGCCCGACATGGACAGTTTTTCATTCTTGCACGTGCTCCTTAAGATGAAAATTGTTGTCATTTTTATGTCATCAGTGATGAACTTGGACGTGGCTACGAAAGCATTGGCTGAAGGAGCGTGTTATTTTCTTCAGAAACCTATTTCCAAGGATGATCTCAAATATGTATGGCAACACGTGTATCGAAGGAACAGAAACATATCAAAGCTAAACCATGAAGCAAATTGCGTAGAAAAAGCCAAATATGGAAAAGAATCTGTCGGCATCCAAAACGACGACACTGTTGTTTTGTCTCAGCCCACCGATGAAGTTAGTATTAATAATAACGGTAGTATTAACTATCAACTTATGAGCTATAAAGAAGAAGGGCAGAATCAACCAACTAATTCCCATGATACCCTAGTTGCTTCATACTTCGAAGGAAAGAGATTAATGGATGACATAGAAGGAACAAGCAAGGAGAAGAGAGTTATATATTACTCAACGCCTACCAATTTTGGAGATACAAGAATTGATGAGGATAATAGAAGAATGAAGGAATATTATATTTCTAGTGATAACAGATCACGTGTCGTTTGGAATGTGGAACGACGTCGCAAATTCTCCGACGCTCTCAACAAGCTAGGAGATAAATGTCGTCCAAAACTTATACTGAAATTGATGAATGAACCATCCTTGACACTGCGCCAGGTAGCTAACCACCTGCAGAAATACAAAGCACAAGTAGAGTCCATGAAAAAGGGGAGAGAAAATAAATTAGCTCCAAGGAGGGAAGCTTCCAAATTCAATTTCTCAGTCAGGACTCAACTTCCTCTTCCGTTGGTACCAAAGCACCCTCACGATGAGGCAAACAGCTCTACAAAGGGAAGTTTAACCTCTTTTATAGGAGGGGAAAGATTCCGACTTATTGCTCCTAAACCTGTGCCTAGTCCTAAACTGCCTGTTAGCGCCAACTTTGCAAATCATGGTCTCATAATGCTTGGCCAAAATTTTCAACCTGTTGATTCAAACTACAATTCAGTTCCATATATCATCAATCAGACAACTCCAAAGGTAACTTCTTCTTGTGTATTTGATGAAACTCAGTTCTCAGATAATCATCTTGATGTAGTACAGCAAGATTTGACTACTTTCAAGATTGAAAACCAAGAAGCAATGGGATGTAATGGCACTTCAGAAGGGATTCAGTTGATGTCAAACAACATGGCTTTTCCTGATTCAACCAACTTGGACAATATTGCTGCAAATTTTGGAAATGAAGGAAGCCAACAACAATCAGCCGAATTTGATTATCTCTTAAATTTTTTAGAAGATGATCCACACGAGTTTGACAGTGACTTAAATCTGAGTGACGTAGATAAGTACAGCGAGTGGCTAAAGAACACTGTTCTTGAAAATAGAAGCGGTCCAGACAGCTTTCTTGGTGACAGTGCAGAAAACTTTTCAACTGAAAACACCCAATAA

Coding sequence (CDS)

ATGCAGAAAATGGGTCATTGGGTCCCTGATTCTCTTCCTAGCTTTGCCAAAAATCTTCATATCTTGGTGGTGGATCATGAAACCTTATCTCTCAACCACTTGAGTTCCTTGCTTAGGGAGCAGTCTTACCATGTTACGACCACTGTATTGGGATCTATTGCTCTATCAATGATTCAAGAAAAAAGTGATGAGTTCGACCTTGTCATGGCTAACGTTAGCATGCCCGACATGGACAGTTTTTCATTCTTGCACGTGCTCCTTAAGATGAAAATTGTTGTCATTTTTATGTCATCAGTGATGAACTTGGACGTGGCTACGAAAGCATTGGCTGAAGGAGCGTGTTATTTTCTTCAGAAACCTATTTCCAAGGATGATCTCAAATATGTATGGCAACACGTGTATCGAAGGAACAGAAACATATCAAAGCTAAACCATGAAGCAAATTGCGTAGAAAAAGCCAAATATGGAAAAGAATCTGTCGGCATCCAAAACGACGACACTGTTGTTTTGTCTCAGCCCACCGATGAAGTTAGTATTAATAATAACGGTAGTATTAACTATCAACTTATGAGCTATAAAGAAGAAGGGCAGAATCAACCAACTAATTCCCATGATACCCTAGTTGCTTCATACTTCGAAGGAAAGAGATTAATGGATGACATAGAAGGAACAAGCAAGGAGAAGAGAGTTATATATTACTCAACGCCTACCAATTTTGGAGATACAAGAATTGATGAGGATAATAGAAGAATGAAGGAATATTATATTTCTAGTGATAACAGATCACGTGTCGTTTGGAATGTGGAACGACGTCGCAAATTCTCCGACGCTCTCAACAAGCTAGGAGATAAATGTCGTCCAAAACTTATACTGAAATTGATGAATGAACCATCCTTGACACTGCGCCAGGTAGCTAACCACCTGCAGAAATACAAAGCACAAGTAGAGTCCATGAAAAAGGGGAGAGAAAATAAATTAGCTCCAAGGAGGGAAGCTTCCAAATTCAATTTCTCAGTCAGGACTCAACTTCCTCTTCCGTTGGTACCAAAGCACCCTCACGATGAGGCAAACAGCTCTACAAAGGGAAGTTTAACCTCTTTTATAGGAGGGGAAAGATTCCGACTTATTGCTCCTAAACCTGTGCCTAGTCCTAAACTGCCTGTTAGCGCCAACTTTGCAAATCATGGTCTCATAATGCTTGGCCAAAATTTTCAACCTGTTGATTCAAACTACAATTCAGTTCCATATATCATCAATCAGACAACTCCAAAGGTAACTTCTTCTTGTGTATTTGATGAAACTCAGTTCTCAGATAATCATCTTGATGTAGTACAGCAAGATTTGACTACTTTCAAGATTGAAAACCAAGAAGCAATGGGATGTAATGGCACTTCAGAAGGGATTCAGTTGATGTCAAACAACATGGCTTTTCCTGATTCAACCAACTTGGACAATATTGCTGCAAATTTTGGAAATGAAGGAAGCCAACAACAATCAGCCGAATTTGATTATCTCTTAAATTTTTTAGAAGATGATCCACACGAGTTTGACAGTGACTTAAATCTGAGTGACGTAGATAAGTACAGCGAGTGGCTAAAGAACACTGTTCTTGAAAATAGAAGCGGTCCAGACAGCTTTCTTGGTGACAGTGCAGAAAACTTTTCAACTGAAAACACCCAATAA

Protein sequence

MQKMGHWVPDSLPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTLRQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTKGSLTSFIGGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQTTPKVTSSCVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDSTNLDNIAANFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRSGPDSFLGDSAENFSTENTQ
Homology
BLAST of CmaCh04G020800 vs. ExPASy Swiss-Prot
Match: Q9C9F6 (Putative two-component response regulator-like APRR6 OS=Arabidopsis thaliana OX=3702 GN=APRR6 PE=3 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 4.2e-32
Identity = 127/452 (28.10%), Postives = 216/452 (47.79%), Query Frame = 0

Query: 12  LPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMAN 71
           +P  A ++ IL++DH+T S+  L+S+L++ S  V +  + S ALSMI+++  E  L++AN
Sbjct: 6   VPECADDISILLIDHDTASIASLTSMLQQFSKRVMSVDVASKALSMIEKQKKEIGLIIAN 65

Query: 72  VSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQ 131
           + MP +DS SFL+ LL   I +I ++  +     +  L + AC+ L KPIS DD+K +WQ
Sbjct: 66  IEMPHIDSHSFLNALLLKDIPLILINPEIKTKEPSDLLTKRACFSLDKPISNDDIKNMWQ 125

Query: 132 HVY-RRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLM 191
           HV+ ++++ + K+N                 I  D   V+ + T            YQ+ 
Sbjct: 126 HVFSKKSQELKKIN-----------------ITEDQENVMDKDT------------YQIE 185

Query: 192 SYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGT----SKEKRV--IYYSTPTNFGDTRI 251
           +++   + Q  +    L      G+R   D   T     K K +  + + T  ++    I
Sbjct: 186 AFRANLKRQRISQASLL------GRRPFIDTFSTYETFQKRKSIANVEWKTTPSYA---I 245

Query: 252 DEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDK-CRPKLILKLMNEPSLTLRQ 311
           + +N+R +  +  S  R + +WN ER  KF  A++ LG++  RPK IL++MN+P+LT RQ
Sbjct: 246 EIENKRKE--WKKSVGRRKSLWNSERHMKFIAAISILGEEDFRPKSILEIMNDPNLTHRQ 305

Query: 312 VANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHD--------- 371
           V +HLQKYKAQ++ +     +   PR E+     S+      P   K+P           
Sbjct: 306 VGSHLQKYKAQIDQI-----SYTLPRNESR----SIDKTFEYPSNYKYPFKISDLTNNLI 365

Query: 372 ---------EANSSTKGSLTSFIGGERFRLIAPKPVPSPKLPVS-----ANFANHGLIM- 429
                    E  +S   S+T F+     + I  K    PK  +      +N + HG ++ 
Sbjct: 366 VSNSLWNSLEKKNSASASITQFLFK---KPIGEKEETMPKFHIGGKLDLSNHSVHGNVLN 405

BLAST of CmaCh04G020800 vs. ExPASy Swiss-Prot
Match: Q5N6V8 (Two-component response regulator ORR26 OS=Oryza sativa subsp. japonica OX=39947 GN=RR26 PE=3 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 6.0e-31
Identity = 113/392 (28.83%), Postives = 187/392 (47.70%), Query Frame = 0

Query: 14  SFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMANVS 73
           +F   L +LVVD +   L  L  +LR+ SY VTT  L  +AL +++E+ ++FD+V+++V+
Sbjct: 5   AFPYGLRVLVVDDDPTWLKILEKMLRKCSYEVTTCGLARVALDILRERKNKFDIVISDVN 64

Query: 74  MPDMDSFSFL-HVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQH 133
           MPDMD F  L H+ L+M + VI MS         K +  GAC +L KP+   +L+ +WQH
Sbjct: 65  MPDMDGFKLLEHIGLEMDLPVIMMSIDGETSRVMKGVQHGACDYLLKPVRMKELRNIWQH 124

Query: 134 VYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLMSY 193
           VYR      K  HE   +E    G +S               D++ I             
Sbjct: 125 VYR------KKMHEVKEIE----GNDS--------------CDDLQI------------- 184

Query: 194 KEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDNRRMK 253
                                   L +  EG  ++   +   + T      +D+D+   +
Sbjct: 185 ------------------------LRNSFEGLDEKSLFMRSDSDTMRKRKDVDKDHADQE 244

Query: 254 EYYISSDNRSRVVWNVERRRKFSDALNKLG-DKCRPKLILKLMNEPSLTLRQVANHLQKY 313
               ++  ++RVVW+V+  +KF +A+N++G DK  PK IL LMN P LT   VA+HLQKY
Sbjct: 245 SSDGNTVKKARVVWSVDLHQKFVNAVNQIGFDKVGPKKILDLMNVPGLTRENVASHLQKY 304

Query: 314 KAQVESMKKGRENKL--APRREAS----KFNFSVRT---QLPLPLVPKHPHDEANSSTKG 373
           +  +  ++K  E ++  A R++ S      N ++R+   + P  +   +PH   N  T+ 
Sbjct: 305 RLYLSRLQKQNEERILGAARQDFSHKGTSENLNLRSSFQEQPSNIANGYPHASQNIQTQA 334

Query: 374 SLTSFIGGERFRLIAPKPVPSPKLPVSANFAN 395
           ++      E  +   P PVP  K  ++++ A+
Sbjct: 365 NMLD-SQLEDTKSTVPLPVPDKKRTLASDAAD 334

BLAST of CmaCh04G020800 vs. ExPASy Swiss-Prot
Match: B8B3I4 (Two-component response regulator ORR22 OS=Oryza sativa subsp. indica OX=39946 GN=RR22 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.7e-28
Identity = 102/306 (33.33%), Postives = 145/306 (47.39%), Query Frame = 0

Query: 15  FAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMANVSM 74
           F   + +L VD + + L  L +LLR   YHVT+T     AL +++E  D FDLV+++V M
Sbjct: 22  FPVGMRVLAVDDDPVCLKVLETLLRRCQYHVTSTNQAITALKLLRENRDMFDLVISDVHM 81

Query: 75  PDMDSFSFLHVL-LKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQHV 134
           PDMD F  L ++ L+M + VI +S         K +  GAC +L KP+  ++L+ +WQHV
Sbjct: 82  PDMDGFKLLELVGLEMDLPVIMLSVNGETKTVMKGITHGACDYLLKPVRIEELRNIWQHV 141

Query: 135 YRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLMSYK 194
            RR       N E N ++   + KE    Q+ DT                          
Sbjct: 142 VRRKFG----NRERNNLD---FSKECNKPQSADT-------------------------- 201

Query: 195 EEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDNRRMKE 254
           + G  QPT                  D  G S  KR   +    + GD     DN   + 
Sbjct: 202 DHGPYQPTCG--------------SSDQNGRSSRKRKELHGEDDDEGD-----DNDYQEN 261

Query: 255 YYISSDNRSRVVWNVERRRKFSDALNKLG-DKCRPKLILKLMNEPSLTLRQVANHLQKYK 314
              S+  + RVVW+VE  RKF  A+N+LG DK  PK IL+LMN   LT   VA+HLQKY+
Sbjct: 262 DEPSAAKKPRVVWSVELHRKFVAAVNQLGIDKAVPKRILELMNVEKLTRENVASHLQKYR 275

Query: 315 AQVESM 319
             ++ +
Sbjct: 322 LYLKRL 275

BLAST of CmaCh04G020800 vs. ExPASy Swiss-Prot
Match: Q5SML5 (Two-component response regulator ORR22 OS=Oryza sativa subsp. japonica OX=39947 GN=RR22 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.7e-28
Identity = 102/306 (33.33%), Postives = 145/306 (47.39%), Query Frame = 0

Query: 15  FAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMANVSM 74
           F   + +L VD + + L  L +LLR   YHVT+T     AL +++E  D FDLV+++V M
Sbjct: 22  FPVGMRVLAVDDDPVCLKVLETLLRRCQYHVTSTNQAITALKLLRENRDMFDLVISDVHM 81

Query: 75  PDMDSFSFLHVL-LKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQHV 134
           PDMD F  L ++ L+M + VI +S         K +  GAC +L KP+  ++L+ +WQHV
Sbjct: 82  PDMDGFKLLELVGLEMDLPVIMLSVNGETKTVMKGITHGACDYLLKPVRIEELRNIWQHV 141

Query: 135 YRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLMSYK 194
            RR       N E N ++   + KE    Q+ DT                          
Sbjct: 142 VRRKFG----NRERNNLD---FSKECNKPQSADT-------------------------- 201

Query: 195 EEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDNRRMKE 254
           + G  QPT                  D  G S  KR   +    + GD     DN   + 
Sbjct: 202 DHGPYQPTCG--------------SSDQNGRSSRKRKELHGEDDDEGD-----DNDYQEN 261

Query: 255 YYISSDNRSRVVWNVERRRKFSDALNKLG-DKCRPKLILKLMNEPSLTLRQVANHLQKYK 314
              S+  + RVVW+VE  RKF  A+N+LG DK  PK IL+LMN   LT   VA+HLQKY+
Sbjct: 262 DEPSAAKKPRVVWSVELHRKFVAAVNQLGIDKAVPKRILELMNVEKLTRENVASHLQKYR 275

Query: 315 AQVESM 319
             ++ +
Sbjct: 322 LYLKRL 275

BLAST of CmaCh04G020800 vs. ExPASy Swiss-Prot
Match: A2X1N2 (Two-component response regulator ORR24 OS=Oryza sativa subsp. indica OX=39946 GN=RR24 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.8e-27
Identity = 99/306 (32.35%), Postives = 143/306 (46.73%), Query Frame = 0

Query: 15  FAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMANVSM 74
           F   + +L VD +   L  L +LL    YHVTTT   + AL +++E  D+FDLV+++V M
Sbjct: 25  FPVGMRVLAVDDDPTCLKILENLLLRCQYHVTTTGQAATALKLLRENKDQFDLVISDVHM 84

Query: 75  PDMDSFSFLHVL-LKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQHV 134
           PDMD F  L ++ L+M + VI +S+        K +  GAC +L KP+  + L+ +WQHV
Sbjct: 85  PDMDGFKLLELVGLEMDLPVIMLSANGETQTVMKGITHGACDYLLKPVRLEQLRTIWQHV 144

Query: 135 YRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLMSYK 194
            RR           NC  K +         NDD                          K
Sbjct: 145 IRRK----------NCDAKNR--------GNDDDA----------------------GQK 204

Query: 195 EEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDNRRMKE 254
            +G N              EG+ +     G ++ KR    S   N GD   D D    + 
Sbjct: 205 AQGMNN-------------EGESI-----GANRNKRQSRKSRDEN-GDDGDDSDENSNEN 264

Query: 255 YYISSDNRSRVVWNVERRRKFSDALNKLG-DKCRPKLILKLMNEPSLTLRQVANHLQKYK 314
              S+  + RVVW+VE  RKF  A+N+LG +K  PK IL LMN  ++T   VA+HLQKY+
Sbjct: 265 GDSSTQKKPRVVWSVELHRKFVAAVNQLGIEKAVPKKILDLMNVENITRENVASHLQKYR 271

Query: 315 AQVESM 319
             ++ +
Sbjct: 325 LYLKRL 271

BLAST of CmaCh04G020800 vs. ExPASy TrEMBL
Match: A0A6J1GRD9 (two-component response regulator ORR26-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456349 PE=4 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 3.7e-281
Identity = 501/557 (89.95%), Postives = 517/557 (92.82%), Query Frame = 0

Query: 2   QKMGHWVPDSLPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEK 61
           Q+MGHWVPDSLPSFAKNLHILVVDHETLSL HLSSLLREQSY+VT T LGSIALSMI+E+
Sbjct: 6   QEMGHWVPDSLPSFAKNLHILVVDHETLSLKHLSSLLREQSYNVTATGLGSIALSMIKER 65

Query: 62  SDEFDLVMANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPI 121
            D+FDLVMANVSMPDMDSFSFLHVLL+M IVVIFMSSVMNLDVAT+ALAEGACYFLQKPI
Sbjct: 66  GDQFDLVMANVSMPDMDSFSFLHVLLEMNIVVIFMSSVMNLDVATRALAEGACYFLQKPI 125

Query: 122 SKDDLKYVWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINN 181
           SKDDLKYVWQHVYRRNRNISKL HEANCVEKAK GKESVGIQNDDT+VLSQ TDEVSINN
Sbjct: 126 SKDDLKYVWQHVYRRNRNISKLTHEANCVEKAKSGKESVGIQNDDTIVLSQSTDEVSINN 185

Query: 182 NGSINYQLMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGD 241
           N SINYQLMSY EE QNQPTN      ASYFEGKRL DDIEGTSKE RV YYSTPTNFGD
Sbjct: 186 NCSINYQLMSYNEEVQNQPTN------ASYFEGKRLTDDIEGTSKENRVTYYSTPTNFGD 245

Query: 242 TRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTL 301
           TRIDEDNRRMKEYYISSDN+SRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEP LTL
Sbjct: 246 TRIDEDNRRMKEYYISSDNKSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPCLTL 305

Query: 302 RQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTK 361
           RQVANHLQKYKAQVESMKKGRENKLAPRRE SKFNFSVRTQLP PLVPK PHDEANSSTK
Sbjct: 306 RQVANHLQKYKAQVESMKKGRENKLAPRRETSKFNFSVRTQLPPPLVPKQPHDEANSSTK 365

Query: 362 GSLTSFIGGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQT 421
           G  TSFIGGERFRLIAP+PVP+P LPVSANFANHGLIML QNF+ VDSNY SVPYIINQT
Sbjct: 366 GGSTSFIGGERFRLIAPRPVPNPTLPVSANFANHGLIMLDQNFRHVDSNYYSVPYIINQT 425

Query: 422 TPKVTSSCVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDST 481
           TP+VTSSCVFD TQFSDN LDVVQQDLT FKI NQEAM CNGTSEGIQLMSNNMAFP ST
Sbjct: 426 TPEVTSSCVFDGTQFSDNPLDVVQQDLTAFKIGNQEAMECNGTSEGIQLMSNNMAFPYST 485

Query: 482 NLDNIAANFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRS 541
           NLDNIAANFGNEGSQQQSAE+DYLLNFLEDDPH+FDSDLNLSDVDKYSEWLKNTVLENRS
Sbjct: 486 NLDNIAANFGNEGSQQQSAEYDYLLNFLEDDPHDFDSDLNLSDVDKYSEWLKNTVLENRS 545

Query: 542 GPDSFLGDSAENFSTEN 559
           GPDSFLGD+ ENF   N
Sbjct: 546 GPDSFLGDNTENFPMGN 556

BLAST of CmaCh04G020800 vs. ExPASy TrEMBL
Match: A0A6J1I817 (putative two-component response regulator-like APRR6 OS=Cucurbita maxima OX=3661 GN=LOC111470486 PE=4 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 6.2e-281
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 69  MANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKY 128
           MANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKY
Sbjct: 1   MANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKY 60

Query: 129 VWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQ 188
           VWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQ
Sbjct: 61  VWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQ 120

Query: 189 LMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDN 248
           LMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDN
Sbjct: 121 LMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDN 180

Query: 249 RRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTLRQVANHL 308
           RRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTLRQVANHL
Sbjct: 181 RRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTLRQVANHL 240

Query: 309 QKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTKGSLTSFI 368
           QKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTKGSLTSFI
Sbjct: 241 QKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTKGSLTSFI 300

Query: 369 GGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQTTPKVTSS 428
           GGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQTTPKVTSS
Sbjct: 301 GGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQTTPKVTSS 360

Query: 429 CVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDSTNLDNIAA 488
           CVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDSTNLDNIAA
Sbjct: 361 CVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDSTNLDNIAA 420

Query: 489 NFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRSGPDSFLG 548
           NFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRSGPDSFLG
Sbjct: 421 NFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRSGPDSFLG 480

Query: 549 DSAENFSTENTQ 561
           DSAENFSTENTQ
Sbjct: 481 DSAENFSTENTQ 492

BLAST of CmaCh04G020800 vs. ExPASy TrEMBL
Match: A0A6J1GRF3 (two-component response regulator ORR26-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456349 PE=4 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.7e-252
Identity = 451/502 (89.84%), Postives = 465/502 (92.63%), Query Frame = 0

Query: 57  MIQEKSDEFDLVMANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYF 116
           MI+E+ D+FDLVMANVSMPDMDSFSFLHVLL+M IVVIFMSSVMNLDVAT+ALAEGACYF
Sbjct: 1   MIKERGDQFDLVMANVSMPDMDSFSFLHVLLEMNIVVIFMSSVMNLDVATRALAEGACYF 60

Query: 117 LQKPISKDDLKYVWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDE 176
           LQKPISKDDLKYVWQHVYRRNRNISKL HEANCVEKAK GKESVGIQNDDT+VLSQ TDE
Sbjct: 61  LQKPISKDDLKYVWQHVYRRNRNISKLTHEANCVEKAKSGKESVGIQNDDTIVLSQSTDE 120

Query: 177 VSINNNGSINYQLMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTP 236
           VSINNN SINYQLMSY EE QNQPTN      ASYFEGKRL DDIEGTSKE RV YYSTP
Sbjct: 121 VSINNNCSINYQLMSYNEEVQNQPTN------ASYFEGKRLTDDIEGTSKENRVTYYSTP 180

Query: 237 TNFGDTRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNE 296
           TNFGDTRIDEDNRRMKEYYISSDN+SRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNE
Sbjct: 181 TNFGDTRIDEDNRRMKEYYISSDNKSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNE 240

Query: 297 PSLTLRQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEA 356
           P LTLRQVANHLQKYKAQVESMKKGRENKLAPRRE SKFNFSVRTQLP PLVPK PHDEA
Sbjct: 241 PCLTLRQVANHLQKYKAQVESMKKGRENKLAPRRETSKFNFSVRTQLPPPLVPKQPHDEA 300

Query: 357 NSSTKGSLTSFIGGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPY 416
           NSSTKG  TSFIGGERFRLIAP+PVP+P LPVSANFANHGLIML QNF+ VDSNY SVPY
Sbjct: 301 NSSTKGGSTSFIGGERFRLIAPRPVPNPTLPVSANFANHGLIMLDQNFRHVDSNYYSVPY 360

Query: 417 IINQTTPKVTSSCVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMA 476
           IINQTTP+VTSSCVFD TQFSDN LDVVQQDLT FKI NQEAM CNGTSEGIQLMSNNMA
Sbjct: 361 IINQTTPEVTSSCVFDGTQFSDNPLDVVQQDLTAFKIGNQEAMECNGTSEGIQLMSNNMA 420

Query: 477 FPDSTNLDNIAANFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTV 536
           FP STNLDNIAANFGNEGSQQQSAE+DYLLNFLEDDPH+FDSDLNLSDVDKYSEWLKNTV
Sbjct: 421 FPYSTNLDNIAANFGNEGSQQQSAEYDYLLNFLEDDPHDFDSDLNLSDVDKYSEWLKNTV 480

Query: 537 LENRSGPDSFLGDSAENFSTEN 559
           LENRSGPDSFLGD+ ENF   N
Sbjct: 481 LENRSGPDSFLGDNTENFPMGN 496

BLAST of CmaCh04G020800 vs. ExPASy TrEMBL
Match: A0A6J1GM46 (putative two-component response regulator-like APRR6 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455231 PE=4 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.8e-220
Identity = 417/579 (72.02%), Postives = 461/579 (79.62%), Query Frame = 0

Query: 1   MQKMGHWVPDSLPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQE 60
           M +M HWVPDSLPSFAK LHILVVDHETLSL HLSSLLR+QSY+VTTT LG IALSMIQE
Sbjct: 1   MHEMDHWVPDSLPSFAKKLHILVVDHETLSLKHLSSLLRQQSYNVTTTGLGCIALSMIQE 60

Query: 61  KSDEFDLVMANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKP 120
           K D+FDLVMANVSMPDMDSFSFLH+LLKM I VIFMSS MNL VATKALAEGACYFLQKP
Sbjct: 61  KGDQFDLVMANVSMPDMDSFSFLHMLLKMNITVIFMSSTMNLKVATKALAEGACYFLQKP 120

Query: 121 ISKDDLKYVWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSIN 180
           ISKDDLKYVWQHVYR N NI+KL  +ANCV+KAKYG+E VG QN + VVLSQ TD  S N
Sbjct: 121 ISKDDLKYVWQHVYRSNINIAKLTDKANCVDKAKYGQEFVGFQNGNPVVLSQSTDAASYN 180

Query: 181 NNGSINYQLMSYKEEGQNQPTNSHDTLVASYFEGKRLM-DDIEGTSKEKRVIYYSTPTNF 240
           NN SINYQLMS+ E+ QN  TNSHDTLVASYFEG+R   DD+EGT+KE+RV YYS PTNF
Sbjct: 181 NNYSINYQLMSHNEKVQNMSTNSHDTLVASYFEGERSADDDVEGTNKEERVTYYSKPTNF 240

Query: 241 GDTRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSL 300
           G T+ DEDN R KEYYIS + RSR+VWN+ERRRKFSDALNKLGDKCRPKLILKLMNEP L
Sbjct: 241 GHTKADEDNGRGKEYYISRNTRSRIVWNLERRRKFSDALNKLGDKCRPKLILKLMNEPCL 300

Query: 301 TLRQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANS- 360
           TLRQVANHLQKYKAQVES++KGR+NKLAPR EASKF+ SV TQLP P V +H + EAN+ 
Sbjct: 301 TLRQVANHLQKYKAQVESIRKGRKNKLAPRMEASKFDCSVTTQLP-PSVQRHQNHEANNS 360

Query: 361 -------------------STKGSLTSFIGGERFRLIAPKPVPSPKLPVSANFANHGLIM 420
                              ST+G  TS  GG+RFRLIAPKP  +P+L VSANF NHGL M
Sbjct: 361 IQQNHEANKSTQQNHETNKSTQGGSTSLSGGKRFRLIAPKPPLNPRLLVSANFVNHGLRM 420

Query: 421 LGQNFQPVDSNYNSVPYIINQTTPKVTSSCVFDETQFSDNH-LDVVQQDLTTFKIENQEA 480
           L  NFQ V SNYNSVPY IN+TTP+VTS   FDE QF D H +D  QQ          EA
Sbjct: 421 LDHNFQHVASNYNSVPYNINKTTPEVTSYRAFDEIQFPDIHQVDAAQQ---------LEA 480

Query: 481 MGCNGTSEGIQLMSNNMAFPDSTNLDNIAANFGNEGSQQQSAEFDYLLNFLEDDPHEFDS 540
           M   GTSEGIQLMS N+A P STNLDNIAANFGNEG QQQ AE+DYLLN LEDDP++F +
Sbjct: 481 MEFCGTSEGIQLMSKNIALPGSTNLDNIAANFGNEGIQQQFAEYDYLLNVLEDDPYDFRN 540

Query: 541 DLNLSDVDKYSEWLKNTVLENRSGPDSFLGDSAENFSTE 558
           D +LSDVDKYSEWL+NT+LE+ SG DSF+ D AENF  E
Sbjct: 541 DFSLSDVDKYSEWLRNTILEDESGLDSFINDEAENFPVE 569

BLAST of CmaCh04G020800 vs. ExPASy TrEMBL
Match: A0A6J1GL19 (putative two-component response regulator-like APRR6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455231 PE=4 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 2.4e-216
Identity = 414/579 (71.50%), Postives = 458/579 (79.10%), Query Frame = 0

Query: 1   MQKMGHWVPDSLPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQE 60
           M +M HWVPDSLPSFAK LHILVVDHETLSL HLSSLLR+QSY+VTTT LG IALSMIQE
Sbjct: 1   MHEMDHWVPDSLPSFAKKLHILVVDHETLSLKHLSSLLRQQSYNVTTTGLGCIALSMIQE 60

Query: 61  KSDEFDLVMANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKP 120
           K D+FDLVMANVSMPDMDSFSFLH+LLKM I VIFMSS MNL VATKALAEGACYFLQKP
Sbjct: 61  KGDQFDLVMANVSMPDMDSFSFLHMLLKMNITVIFMSSTMNLKVATKALAEGACYFLQKP 120

Query: 121 ISKDDLKYVWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSIN 180
           ISKDDLKYVWQHVYR N NI+KL  +ANCV+KAKYG+E VG QN + VVLSQ TD  S N
Sbjct: 121 ISKDDLKYVWQHVYRSNINIAKLTDKANCVDKAKYGQEFVGFQNGNPVVLSQSTDAASYN 180

Query: 181 NNGSINYQLMSYKEEGQNQPTNSHDTLVASYFEGKRLM-DDIEGTSKEKRVIYYSTPTNF 240
           NN SINYQLMS+ E+ QN  TNSHDTLVASYFEG+R   DD+EGT+KE+RV YYS PTNF
Sbjct: 181 NNYSINYQLMSHNEKVQNMSTNSHDTLVASYFEGERSADDDVEGTNKEERVTYYSKPTNF 240

Query: 241 GDTRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSL 300
           G T+ DEDN R KEYYIS + RSR+VWN+ERRRKFSDALNKLG   RPKLILKLMNEP L
Sbjct: 241 GHTKADEDNGRGKEYYISRNTRSRIVWNLERRRKFSDALNKLG---RPKLILKLMNEPCL 300

Query: 301 TLRQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANS- 360
           TLRQVANHLQKYKAQVES++KGR+NKLAPR EASKF+ SV TQLP P V +H + EAN+ 
Sbjct: 301 TLRQVANHLQKYKAQVESIRKGRKNKLAPRMEASKFDCSVTTQLP-PSVQRHQNHEANNS 360

Query: 361 -------------------STKGSLTSFIGGERFRLIAPKPVPSPKLPVSANFANHGLIM 420
                              ST+G  TS  GG+RFRLIAPKP  +P+L VSANF NHGL M
Sbjct: 361 IQQNHEANKSTQQNHETNKSTQGGSTSLSGGKRFRLIAPKPPLNPRLLVSANFVNHGLRM 420

Query: 421 LGQNFQPVDSNYNSVPYIINQTTPKVTSSCVFDETQFSDNH-LDVVQQDLTTFKIENQEA 480
           L  NFQ V SNYNSVPY IN+TTP+VTS   FDE QF D H +D  QQ          EA
Sbjct: 421 LDHNFQHVASNYNSVPYNINKTTPEVTSYRAFDEIQFPDIHQVDAAQQ---------LEA 480

Query: 481 MGCNGTSEGIQLMSNNMAFPDSTNLDNIAANFGNEGSQQQSAEFDYLLNFLEDDPHEFDS 540
           M   GTSEGIQLMS N+A P STNLDNIAANFGNEG QQQ AE+DYLLN LEDDP++F +
Sbjct: 481 MEFCGTSEGIQLMSKNIALPGSTNLDNIAANFGNEGIQQQFAEYDYLLNVLEDDPYDFRN 540

Query: 541 DLNLSDVDKYSEWLKNTVLENRSGPDSFLGDSAENFSTE 558
           D +LSDVDKYSEWL+NT+LE+ SG DSF+ D AENF  E
Sbjct: 541 DFSLSDVDKYSEWLRNTILEDESGLDSFINDEAENFPVE 566

BLAST of CmaCh04G020800 vs. NCBI nr
Match: XP_023550077.1 (two-component response regulator ORR26-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 988.0 bits (2553), Expect = 3.3e-284
Identity = 504/557 (90.48%), Postives = 522/557 (93.72%), Query Frame = 0

Query: 2   QKMGHWVPDSLPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEK 61
           Q+MGHWVPDSLPSFAKNLHILVVDHETLSL HLSSLLREQSY+VT T LGSIALSMI+E+
Sbjct: 3   QEMGHWVPDSLPSFAKNLHILVVDHETLSLKHLSSLLREQSYNVTATGLGSIALSMIKER 62

Query: 62  SDEFDLVMANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPI 121
            D+FDLVMANVSMPDMDSF FLHVLL+M IVVIFMSSVM+LDVAT+ALAEGACYFLQKPI
Sbjct: 63  GDQFDLVMANVSMPDMDSFWFLHVLLEMNIVVIFMSSVMDLDVATRALAEGACYFLQKPI 122

Query: 122 SKDDLKYVWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINN 181
           SKDDLKYVWQHVYRRNRNISKL HEANC EKAK GKESVGIQNDDT+VLSQ TDEVSINN
Sbjct: 123 SKDDLKYVWQHVYRRNRNISKLTHEANCGEKAKSGKESVGIQNDDTIVLSQSTDEVSINN 182

Query: 182 NGSINYQLMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGD 241
           N SINYQLMSY EE QNQPTNSHDT VASYFEGKRL DDIEGTSKEKRV YYSTPTNFGD
Sbjct: 183 NCSINYQLMSYNEEVQNQPTNSHDTQVASYFEGKRLTDDIEGTSKEKRVTYYSTPTNFGD 242

Query: 242 TRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTL 301
           TRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEP LTL
Sbjct: 243 TRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPCLTL 302

Query: 302 RQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTK 361
           RQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLP PLVPK PHDEAN ST 
Sbjct: 303 RQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPPPLVPKQPHDEANRSTD 362

Query: 362 GSLTSFIGGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQT 421
           G  TSFIGGERFRLIAP+PVP+P LPVSANFANHGLIML QN+Q V+SNY SVPYIINQT
Sbjct: 363 GGSTSFIGGERFRLIAPRPVPNPTLPVSANFANHGLIMLDQNYQHVNSNYYSVPYIINQT 422

Query: 422 TPKVTSSCVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDST 481
           TP+VTSSCVFDETQFSDN LDVVQQDLT FKIENQEAM  NGTSEGIQLMSNNMAFPDST
Sbjct: 423 TPEVTSSCVFDETQFSDNRLDVVQQDLTAFKIENQEAMEFNGTSEGIQLMSNNMAFPDST 482

Query: 482 NLDNIAANFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRS 541
           N DNIAANFGNEGSQQQSAE+DYLLNFLEDDPH+FDS+LNLSDVDKYSEWLKNTVLENRS
Sbjct: 483 NFDNIAANFGNEGSQQQSAEYDYLLNFLEDDPHDFDSNLNLSDVDKYSEWLKNTVLENRS 542

Query: 542 GPDSFLGDSAENFSTEN 559
           GPDSFLGD+AENF   N
Sbjct: 543 GPDSFLGDNAENFPMGN 559

BLAST of CmaCh04G020800 vs. NCBI nr
Match: XP_022953944.1 (two-component response regulator ORR26-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 976.9 bits (2524), Expect = 7.6e-281
Identity = 501/557 (89.95%), Postives = 517/557 (92.82%), Query Frame = 0

Query: 2   QKMGHWVPDSLPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEK 61
           Q+MGHWVPDSLPSFAKNLHILVVDHETLSL HLSSLLREQSY+VT T LGSIALSMI+E+
Sbjct: 6   QEMGHWVPDSLPSFAKNLHILVVDHETLSLKHLSSLLREQSYNVTATGLGSIALSMIKER 65

Query: 62  SDEFDLVMANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPI 121
            D+FDLVMANVSMPDMDSFSFLHVLL+M IVVIFMSSVMNLDVAT+ALAEGACYFLQKPI
Sbjct: 66  GDQFDLVMANVSMPDMDSFSFLHVLLEMNIVVIFMSSVMNLDVATRALAEGACYFLQKPI 125

Query: 122 SKDDLKYVWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINN 181
           SKDDLKYVWQHVYRRNRNISKL HEANCVEKAK GKESVGIQNDDT+VLSQ TDEVSINN
Sbjct: 126 SKDDLKYVWQHVYRRNRNISKLTHEANCVEKAKSGKESVGIQNDDTIVLSQSTDEVSINN 185

Query: 182 NGSINYQLMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGD 241
           N SINYQLMSY EE QNQPTN      ASYFEGKRL DDIEGTSKE RV YYSTPTNFGD
Sbjct: 186 NCSINYQLMSYNEEVQNQPTN------ASYFEGKRLTDDIEGTSKENRVTYYSTPTNFGD 245

Query: 242 TRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTL 301
           TRIDEDNRRMKEYYISSDN+SRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEP LTL
Sbjct: 246 TRIDEDNRRMKEYYISSDNKSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPCLTL 305

Query: 302 RQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTK 361
           RQVANHLQKYKAQVESMKKGRENKLAPRRE SKFNFSVRTQLP PLVPK PHDEANSSTK
Sbjct: 306 RQVANHLQKYKAQVESMKKGRENKLAPRRETSKFNFSVRTQLPPPLVPKQPHDEANSSTK 365

Query: 362 GSLTSFIGGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQT 421
           G  TSFIGGERFRLIAP+PVP+P LPVSANFANHGLIML QNF+ VDSNY SVPYIINQT
Sbjct: 366 GGSTSFIGGERFRLIAPRPVPNPTLPVSANFANHGLIMLDQNFRHVDSNYYSVPYIINQT 425

Query: 422 TPKVTSSCVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDST 481
           TP+VTSSCVFD TQFSDN LDVVQQDLT FKI NQEAM CNGTSEGIQLMSNNMAFP ST
Sbjct: 426 TPEVTSSCVFDGTQFSDNPLDVVQQDLTAFKIGNQEAMECNGTSEGIQLMSNNMAFPYST 485

Query: 482 NLDNIAANFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRS 541
           NLDNIAANFGNEGSQQQSAE+DYLLNFLEDDPH+FDSDLNLSDVDKYSEWLKNTVLENRS
Sbjct: 486 NLDNIAANFGNEGSQQQSAEYDYLLNFLEDDPHDFDSDLNLSDVDKYSEWLKNTVLENRS 545

Query: 542 GPDSFLGDSAENFSTEN 559
           GPDSFLGD+ ENF   N
Sbjct: 546 GPDSFLGDNTENFPMGN 556

BLAST of CmaCh04G020800 vs. NCBI nr
Match: XP_022971808.1 (putative two-component response regulator-like APRR6 [Cucurbita maxima])

HSP 1 Score: 976.1 bits (2522), Expect = 1.3e-280
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 69  MANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKY 128
           MANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKY
Sbjct: 1   MANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKY 60

Query: 129 VWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQ 188
           VWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQ
Sbjct: 61  VWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQ 120

Query: 189 LMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDN 248
           LMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDN
Sbjct: 121 LMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDN 180

Query: 249 RRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTLRQVANHL 308
           RRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTLRQVANHL
Sbjct: 181 RRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTLRQVANHL 240

Query: 309 QKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTKGSLTSFI 368
           QKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTKGSLTSFI
Sbjct: 241 QKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTKGSLTSFI 300

Query: 369 GGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQTTPKVTSS 428
           GGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQTTPKVTSS
Sbjct: 301 GGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQTTPKVTSS 360

Query: 429 CVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDSTNLDNIAA 488
           CVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDSTNLDNIAA
Sbjct: 361 CVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDSTNLDNIAA 420

Query: 489 NFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRSGPDSFLG 548
           NFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRSGPDSFLG
Sbjct: 421 NFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENRSGPDSFLG 480

Query: 549 DSAENFSTENTQ 561
           DSAENFSTENTQ
Sbjct: 481 DSAENFSTENTQ 492

BLAST of CmaCh04G020800 vs. NCBI nr
Match: KAG7032529.1 (putative two-component response regulator-like APRR6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 941.4 bits (2432), Expect = 3.5e-270
Identity = 491/558 (87.99%), Postives = 511/558 (91.58%), Query Frame = 0

Query: 2   QKMGHWVPDSLPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEK 61
           Q+MGHWVPDSLPSFAKNLHILVVDHET SL HLSSLLR+QSY+VT T LGSIALSMI+E+
Sbjct: 109 QEMGHWVPDSLPSFAKNLHILVVDHETFSLKHLSSLLRKQSYNVTATGLGSIALSMIKER 168

Query: 62  SDEFDLVMANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPI 121
            D+FDLVMANVSMPDMDSFSFLHVLL+M IVVIFMSSVMNLDVAT+ALAEGACYFLQKPI
Sbjct: 169 GDQFDLVMANVSMPDMDSFSFLHVLLEMNIVVIFMSSVMNLDVATRALAEGACYFLQKPI 228

Query: 122 SKDDLKYVWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINN 181
           SK+DLKYVWQHVYRRNRNISKL +EANC EKAK GKESVGIQNDDT+VLSQ TDEVSINN
Sbjct: 229 SKEDLKYVWQHVYRRNRNISKLTYEANCGEKAKSGKESVGIQNDDTIVLSQSTDEVSINN 288

Query: 182 NGSINYQLMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGD 241
           N SINYQLMSY EE QNQPTN      ASYFEGKRL DDIEGTSKEKRV YYSTPTNFGD
Sbjct: 289 NCSINYQLMSYNEEVQNQPTN------ASYFEGKRLTDDIEGTSKEKRVTYYSTPTNFGD 348

Query: 242 TRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNEPSLTL 301
           TRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLG   RPKLILKLMNEP LTL
Sbjct: 349 TRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLG---RPKLILKLMNEPCLTL 408

Query: 302 RQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEANSSTK 361
           RQVANHLQKYKAQVESMKKGRENKLA RREASKFNFSVRTQLP PL+PK PHDEANSSTK
Sbjct: 409 RQVANHLQKYKAQVESMKKGRENKLASRREASKFNFSVRTQLPPPLMPKQPHDEANSSTK 468

Query: 362 GSLTSFIGGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPYIINQT 421
           G  TSFIGGERFRLIAP+PV +P LPVSANFANHGLIML QNFQ VDSNY SVPYIINQ 
Sbjct: 469 GGSTSFIGGERFRLIAPRPVLNPTLPVSANFANHGLIMLDQNFQHVDSNYYSVPYIINQA 528

Query: 422 TPKVTSSCVFDETQFSD-NHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMAFPDS 481
           TP+V SSCVFDETQFS+ N LDVVQQDLT FKI NQEAM CNGTSE IQLMSNNMAFP+S
Sbjct: 529 TPEVNSSCVFDETQFSNYNPLDVVQQDLTAFKIGNQEAMECNGTSERIQLMSNNMAFPNS 588

Query: 482 TNLDNIAANFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTVLENR 541
           TNLDNIAANFGNEGSQQQSAE+DYLLNFLEDDP +FDSDLNLSDVDKYSEWLKNTVLENR
Sbjct: 589 TNLDNIAANFGNEGSQQQSAEYDYLLNFLEDDPQDFDSDLNLSDVDKYSEWLKNTVLENR 648

Query: 542 SGPDSFLGDSAENFSTEN 559
           SGPDSFLGD+AENF   N
Sbjct: 649 SGPDSFLGDNAENFPMGN 657

BLAST of CmaCh04G020800 vs. NCBI nr
Match: XP_022953954.1 (two-component response regulator ORR26-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 880.2 bits (2273), Expect = 9.6e-252
Identity = 451/502 (89.84%), Postives = 465/502 (92.63%), Query Frame = 0

Query: 57  MIQEKSDEFDLVMANVSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYF 116
           MI+E+ D+FDLVMANVSMPDMDSFSFLHVLL+M IVVIFMSSVMNLDVAT+ALAEGACYF
Sbjct: 1   MIKERGDQFDLVMANVSMPDMDSFSFLHVLLEMNIVVIFMSSVMNLDVATRALAEGACYF 60

Query: 117 LQKPISKDDLKYVWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDE 176
           LQKPISKDDLKYVWQHVYRRNRNISKL HEANCVEKAK GKESVGIQNDDT+VLSQ TDE
Sbjct: 61  LQKPISKDDLKYVWQHVYRRNRNISKLTHEANCVEKAKSGKESVGIQNDDTIVLSQSTDE 120

Query: 177 VSINNNGSINYQLMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTP 236
           VSINNN SINYQLMSY EE QNQPTN      ASYFEGKRL DDIEGTSKE RV YYSTP
Sbjct: 121 VSINNNCSINYQLMSYNEEVQNQPTN------ASYFEGKRLTDDIEGTSKENRVTYYSTP 180

Query: 237 TNFGDTRIDEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNE 296
           TNFGDTRIDEDNRRMKEYYISSDN+SRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNE
Sbjct: 181 TNFGDTRIDEDNRRMKEYYISSDNKSRVVWNVERRRKFSDALNKLGDKCRPKLILKLMNE 240

Query: 297 PSLTLRQVANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHDEA 356
           P LTLRQVANHLQKYKAQVESMKKGRENKLAPRRE SKFNFSVRTQLP PLVPK PHDEA
Sbjct: 241 PCLTLRQVANHLQKYKAQVESMKKGRENKLAPRRETSKFNFSVRTQLPPPLVPKQPHDEA 300

Query: 357 NSSTKGSLTSFIGGERFRLIAPKPVPSPKLPVSANFANHGLIMLGQNFQPVDSNYNSVPY 416
           NSSTKG  TSFIGGERFRLIAP+PVP+P LPVSANFANHGLIML QNF+ VDSNY SVPY
Sbjct: 301 NSSTKGGSTSFIGGERFRLIAPRPVPNPTLPVSANFANHGLIMLDQNFRHVDSNYYSVPY 360

Query: 417 IINQTTPKVTSSCVFDETQFSDNHLDVVQQDLTTFKIENQEAMGCNGTSEGIQLMSNNMA 476
           IINQTTP+VTSSCVFD TQFSDN LDVVQQDLT FKI NQEAM CNGTSEGIQLMSNNMA
Sbjct: 361 IINQTTPEVTSSCVFDGTQFSDNPLDVVQQDLTAFKIGNQEAMECNGTSEGIQLMSNNMA 420

Query: 477 FPDSTNLDNIAANFGNEGSQQQSAEFDYLLNFLEDDPHEFDSDLNLSDVDKYSEWLKNTV 536
           FP STNLDNIAANFGNEGSQQQSAE+DYLLNFLEDDPH+FDSDLNLSDVDKYSEWLKNTV
Sbjct: 421 FPYSTNLDNIAANFGNEGSQQQSAEYDYLLNFLEDDPHDFDSDLNLSDVDKYSEWLKNTV 480

Query: 537 LENRSGPDSFLGDSAENFSTEN 559
           LENRSGPDSFLGD+ ENF   N
Sbjct: 481 LENRSGPDSFLGDNTENFPMGN 496

BLAST of CmaCh04G020800 vs. TAIR 10
Match: AT1G68210.1 (pseudo-response regulator 6 )

HSP 1 Score: 141.0 bits (354), Expect = 3.0e-33
Identity = 127/452 (28.10%), Postives = 216/452 (47.79%), Query Frame = 0

Query: 12  LPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMAN 71
           +P  A ++ IL++DH+T S+  L+S+L++ S  V +  + S ALSMI+++  E  L++AN
Sbjct: 6   VPECADDISILLIDHDTASIASLTSMLQQFSKRVMSVDVASKALSMIEKQKKEIGLIIAN 65

Query: 72  VSMPDMDSFSFLHVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQ 131
           + MP +DS SFL+ LL   I +I ++  +     +  L + AC+ L KPIS DD+K +WQ
Sbjct: 66  IEMPHIDSHSFLNALLLKDIPLILINPEIKTKEPSDLLTKRACFSLDKPISNDDIKNMWQ 125

Query: 132 HVY-RRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLM 191
           HV+ ++++ + K+N                 I  D   V+ + T            YQ+ 
Sbjct: 126 HVFSKKSQELKKIN-----------------ITEDQENVMDKDT------------YQIE 185

Query: 192 SYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGT----SKEKRV--IYYSTPTNFGDTRI 251
           +++   + Q  +    L      G+R   D   T     K K +  + + T  ++    I
Sbjct: 186 AFRANLKRQRISQASLL------GRRPFIDTFSTYETFQKRKSIANVEWKTTPSYA---I 245

Query: 252 DEDNRRMKEYYISSDNRSRVVWNVERRRKFSDALNKLGDK-CRPKLILKLMNEPSLTLRQ 311
           + +N+R +  +  S  R + +WN ER  KF  A++ LG++  RPK IL++MN+P+LT RQ
Sbjct: 246 EIENKRKE--WKKSVGRRKSLWNSERHMKFIAAISILGEEDFRPKSILEIMNDPNLTHRQ 305

Query: 312 VANHLQKYKAQVESMKKGRENKLAPRREASKFNFSVRTQLPLPLVPKHPHD--------- 371
           V +HLQKYKAQ++ +     +   PR E+     S+      P   K+P           
Sbjct: 306 VGSHLQKYKAQIDQI-----SYTLPRNESR----SIDKTFEYPSNYKYPFKISDLTNNLI 365

Query: 372 ---------EANSSTKGSLTSFIGGERFRLIAPKPVPSPKLPVS-----ANFANHGLIM- 429
                    E  +S   S+T F+     + I  K    PK  +      +N + HG ++ 
Sbjct: 366 VSNSLWNSLEKKNSASASITQFLFK---KPIGEKEETMPKFHIGGKLDLSNHSVHGNVLN 405

BLAST of CmaCh04G020800 vs. TAIR 10
Match: AT2G25180.1 (response regulator 12 )

HSP 1 Score: 121.3 bits (303), Expect = 2.4e-27
Identity = 97/305 (31.80%), Postives = 137/305 (44.92%), Query Frame = 0

Query: 10  DSLPSFAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVM 69
           ++L  F   + +L VD +   L  L SLLR   YHVTTT     AL +++E  ++FDLV+
Sbjct: 8   EALDQFPVGMRVLAVDDDQTCLKILESLLRHCQYHVTTTNQAQKALELLRENKNKFDLVI 67

Query: 70  ANVSMPDMDSFSFLHVL-LKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKY 129
           ++V MPDMD F  L ++ L+M + VI +S+  +     K +  GAC +L KP+  ++LK 
Sbjct: 68  SDVDMPDMDGFKLLELVGLEMDLPVIMLSAHSDPKYVMKGVTHGACDYLLKPVRIEELKN 127

Query: 130 VWQHVYRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQ 189
           +WQHV R                 +++ K                      NNNG     
Sbjct: 128 IWQHVVR-----------------SRFDKNR------------------GSNNNGD---- 187

Query: 190 LMSYKEEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDN 249
               K +G                EG    D   G    KR   Y+          DED 
Sbjct: 188 ----KRDGSGN-------------EGVGNSDQNNGKGNRKRKDQYNE---------DEDE 247

Query: 250 RRMKEYYISSDNRSRVVWNVERRRKFSDALNKLG-DKCRPKLILKLMNEPSLTLRQVANH 309
            R       +  + RVVW VE  +KF  A+N+LG +K  PK IL LMN   LT   VA+H
Sbjct: 248 DRDDNDDSCAQKKQRVVWTVELHKKFVAAVNQLGYEKAMPKKILDLMNVEKLTRENVASH 247

Query: 310 LQKYK 313
           LQK++
Sbjct: 308 LQKFR 247

BLAST of CmaCh04G020800 vs. TAIR 10
Match: AT3G16857.1 (response regulator 1 )

HSP 1 Score: 119.8 bits (299), Expect = 7.1e-27
Identity = 100/300 (33.33%), Postives = 148/300 (49.33%), Query Frame = 0

Query: 15  FAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMANVSM 74
           F   L +LVVD +   L  L  +LR   Y VT      +ALS++++    FD+V+++V M
Sbjct: 33  FPSGLRVLVVDDDPTCLMILERMLRTCLYEVTKCNRAEMALSLLRKNKHGFDIVISDVHM 92

Query: 75  PDMDSFSFL-HVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQHV 134
           PDMD F  L HV L+M + VI MS+  +  V  K +  GA  +L KP+  + LK +WQHV
Sbjct: 93  PDMDGFKLLEHVGLEMDLPVIMMSADDSKSVVLKGVTHGAVDYLIKPVRMEALKNIWQHV 152

Query: 135 YRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLMSYK 194
            R+ R+   +   +  +E+    ++           +S   D V  +N+ S+N       
Sbjct: 153 VRKRRSEWSVPEHSGSIEETGERQQQQHRGGGGGAAVSGGEDAVD-DNSSSVN------- 212

Query: 195 EEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDNRRMKE 254
            EG N  ++S            R   D EG  +             GD + DED   +K 
Sbjct: 213 -EGNNWRSSS------------RKRKDEEGEEQ-------------GDDK-DEDASNLK- 272

Query: 255 YYISSDNRSRVVWNVERRRKFSDALNKLG-DKCRPKLILKLMNEPSLTLRQVANHLQKYK 313
                  + RVVW+VE  ++F  A+N+LG +K  PK IL+LMN P LT   VA+HLQKY+
Sbjct: 273 -------KPRVVWSVELHQQFVAAVNQLGVEKAVPKKILELMNVPGLTRENVASHLQKYR 289

BLAST of CmaCh04G020800 vs. TAIR 10
Match: AT3G16857.2 (response regulator 1 )

HSP 1 Score: 119.8 bits (299), Expect = 7.1e-27
Identity = 100/300 (33.33%), Postives = 148/300 (49.33%), Query Frame = 0

Query: 15  FAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMANVSM 74
           F   L +LVVD +   L  L  +LR   Y VT      +ALS++++    FD+V+++V M
Sbjct: 33  FPSGLRVLVVDDDPTCLMILERMLRTCLYEVTKCNRAEMALSLLRKNKHGFDIVISDVHM 92

Query: 75  PDMDSFSFL-HVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQHV 134
           PDMD F  L HV L+M + VI MS+  +  V  K +  GA  +L KP+  + LK +WQHV
Sbjct: 93  PDMDGFKLLEHVGLEMDLPVIMMSADDSKSVVLKGVTHGAVDYLIKPVRMEALKNIWQHV 152

Query: 135 YRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLMSYK 194
            R+ R+   +   +  +E+    ++           +S   D V  +N+ S+N       
Sbjct: 153 VRKRRSEWSVPEHSGSIEETGERQQQQHRGGGGGAAVSGGEDAVD-DNSSSVN------- 212

Query: 195 EEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDNRRMKE 254
            EG N  ++S            R   D EG  +             GD + DED   +K 
Sbjct: 213 -EGNNWRSSS------------RKRKDEEGEEQ-------------GDDK-DEDASNLK- 272

Query: 255 YYISSDNRSRVVWNVERRRKFSDALNKLG-DKCRPKLILKLMNEPSLTLRQVANHLQKYK 313
                  + RVVW+VE  ++F  A+N+LG +K  PK IL+LMN P LT   VA+HLQKY+
Sbjct: 273 -------KPRVVWSVELHQQFVAAVNQLGVEKAVPKKILELMNVPGLTRENVASHLQKYR 289

BLAST of CmaCh04G020800 vs. TAIR 10
Match: AT4G16110.1 (response regulator 2 )

HSP 1 Score: 118.2 bits (295), Expect = 2.1e-26
Identity = 100/300 (33.33%), Postives = 145/300 (48.33%), Query Frame = 0

Query: 15  FAKNLHILVVDHETLSLNHLSSLLREQSYHVTTTVLGSIALSMIQEKSDEFDLVMANVSM 74
           F  NL +LVVD +   L  L  +L    Y VT       ALS++++  + FD+V+++V M
Sbjct: 24  FPANLRVLVVDDDPTCLMILERMLMTCLYRVTKCNRAESALSLLRKNKNGFDIVISDVHM 83

Query: 75  PDMDSFSFL-HVLLKMKIVVIFMSSVMNLDVATKALAEGACYFLQKPISKDDLKYVWQHV 134
           PDMD F  L HV L+M + VI MS+  +  V  K +  GA  +L KP+  + LK +WQHV
Sbjct: 84  PDMDGFKLLEHVGLEMDLPVIMMSADDSKSVVLKGVTHGAVDYLIKPVRIEALKNIWQHV 143

Query: 135 YRRNRNISKLNHEANCVEKAKYGKESVGIQNDDTVVLSQPTDEVSINNNGSINYQLMSYK 194
            R+ RN      E N  E +    E  G   D      Q   E + NN+ S+N       
Sbjct: 144 VRKKRN------EWNVSEHSGGSIEDTGGDRD----RQQQHREDADNNSSSVN------- 203

Query: 195 EEGQNQPTNSHDTLVASYFEGKRLMDDIEGTSKEKRVIYYSTPTNFGDTRIDEDNRRMKE 254
            EG  + +             KR  ++++    +K                 ED+  +K 
Sbjct: 204 -EGNGRSSR------------KRKEEEVDDQGDDK-----------------EDSSSLK- 263

Query: 255 YYISSDNRSRVVWNVERRRKFSDALNKLG-DKCRPKLILKLMNEPSLTLRQVANHLQKYK 313
                  + RVVW+VE  ++F  A+N+LG DK  PK IL++MN P LT   VA+HLQKY+
Sbjct: 264 -------KPRVVWSVELHQQFVAAVNQLGVDKAVPKKILEMMNVPGLTRENVASHLQKYR 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9F64.2e-3228.10Putative two-component response regulator-like APRR6 OS=Arabidopsis thaliana OX=... [more]
Q5N6V86.0e-3128.83Two-component response regulator ORR26 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
B8B3I43.7e-2833.33Two-component response regulator ORR22 OS=Oryza sativa subsp. indica OX=39946 GN... [more]
Q5SML53.7e-2833.33Two-component response regulator ORR22 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
A2X1N21.8e-2732.35Two-component response regulator ORR24 OS=Oryza sativa subsp. indica OX=39946 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1GRD93.7e-28189.95two-component response regulator ORR26-like isoform X1 OS=Cucurbita moschata OX=... [more]
A0A6J1I8176.2e-281100.00putative two-component response regulator-like APRR6 OS=Cucurbita maxima OX=3661... [more]
A0A6J1GRF34.7e-25289.84two-component response regulator ORR26-like isoform X2 OS=Cucurbita moschata OX=... [more]
A0A6J1GM462.8e-22072.02putative two-component response regulator-like APRR6 isoform X1 OS=Cucurbita mos... [more]
A0A6J1GL192.4e-21671.50putative two-component response regulator-like APRR6 isoform X2 OS=Cucurbita mos... [more]
Match NameE-valueIdentityDescription
XP_023550077.13.3e-28490.48two-component response regulator ORR26-like [Cucurbita pepo subsp. pepo][more]
XP_022953944.17.6e-28189.95two-component response regulator ORR26-like isoform X1 [Cucurbita moschata][more]
XP_022971808.11.3e-280100.00putative two-component response regulator-like APRR6 [Cucurbita maxima][more]
KAG7032529.13.5e-27087.99putative two-component response regulator-like APRR6, partial [Cucurbita argyros... [more]
XP_022953954.19.6e-25289.84two-component response regulator ORR26-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G68210.13.0e-3328.10pseudo-response regulator 6 [more]
AT2G25180.12.4e-2731.80response regulator 12 [more]
AT3G16857.17.1e-2733.33response regulator 1 [more]
AT3G16857.27.1e-2733.33response regulator 1 [more]
AT4G16110.12.1e-2633.33response regulator 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001789Signal transduction response regulator, receiver domainSMARTSM00448REC_2coord: 19..130
e-value: 1.5E-13
score: 61.0
IPR001789Signal transduction response regulator, receiver domainPFAMPF00072Response_regcoord: 21..127
e-value: 9.8E-15
score: 54.7
IPR001789Signal transduction response regulator, receiver domainPROSITEPS50110RESPONSE_REGULATORYcoord: 20..134
score: 31.487852
NoneNo IPR availableGENE3D3.40.50.2300coord: 18..153
e-value: 1.2E-23
score: 85.4
NoneNo IPR availableGENE3D1.10.10.60coord: 258..318
e-value: 1.4E-16
score: 62.2
NoneNo IPR availablePANTHERPTHR43874TWO-COMPONENT RESPONSE REGULATORcoord: 15..546
NoneNo IPR availablePANTHERPTHR43874:SF87TWO-COMPONENT RESPONSE REGULATOR ARR11-LIKEcoord: 15..546
NoneNo IPR availableCDDcd17584REC_typeB_ARR-likecoord: 21..134
e-value: 4.96645E-34
score: 123.122
IPR006447Myb domain, plantsTIGRFAMTIGR01557TIGR01557coord: 262..313
e-value: 3.8E-12
score: 44.3
IPR009057Homeobox-like domain superfamilySUPERFAMILY46689Homeodomain-likecoord: 261..317
IPR011006CheY-like superfamilySUPERFAMILY52172CheY-likecoord: 17..146

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G020800.1CmaCh04G020800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000160 phosphorelay signal transduction system
biological_process GO:0016310 phosphorylation
molecular_function GO:0003677 DNA binding
molecular_function GO:0016301 kinase activity