CmaCh04G020660 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G020660
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionMannose-6-phosphate isomerase
LocationCma_Chr04: 13864585 .. 13868434 (-)
RNA-Seq ExpressionCmaCh04G020660
SyntenyCmaCh04G020660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGACTACCTCCAGCTATGCTAACCGCTCTCTCCTTCTCTATCTTTCTCTGCTTATATAAGTTATAACGAACACAGGATCCTGAACCTTCTTCAATTCCCAAGAGACTCTCAGTTTCTTCTTCTGAGGCTTTTGATTTTTTTATTTTTGATTTACGTCCATTTTTGTTGTTCTTGGTGATGAGCAATGGCCGAAGAAGATGATAAGAGATTGAAGAAAAGGAAGAAGAAGAGGTTGATAGGCGCCGACAGGAGGAGGCGTCTCCTTCGTCTCAAATGCTGTGTGCAGAACTACGATTGGGGGGTTCTAGGGTGTAATTCACAAGTTGCGAGGATGTTTTTGCTCAATTCGGGCTCCTACAGTGCCGATCCGGATGAATGTTACGCGGAGTTCTGGATTGGAACGCATAAATCGGGTCCGTCTTTCGTTTTTTTTGGACGGGACTATAATGCCGCTGCAATTGGATCTAAACCCTTGAGTCTCAAGGACTGGATCACGGTCGATCCGGGCGCTGTGCTTGGTGATAAGGTTGCGCGGAAATGGGGAGGCGATCTCCCCTTCCTCTTCAAGGTGCGGTTTCTTCTCTCTTAGATCCTAAATGTGTTTTGTCGGTGTACTTGATTCCTTTCAAGATCACTTTAATGGAAAGAAAATCCACATTGTGTCGGTGATAGTTAATCAGGATGCTTAGAATTTGCTGTTAAATGATATTAATGTTTCGTAAGCGAATAAGAAATTGTGTAAAGAATTATCTACTGAATTTGTGCTCCATCTCTTTAAATTGTCAGTTCCATTAGTTTCACGAACCTCAATTACCGTTCTTATTTTACTTGACGTTAATGTTTAGAGCTCAGTTGGACTGCAACTTGATGATTTCTTATTGGGAGCATAGGTGCTGTCGGTAGAGAAGGCTTTATCTATTCAGGCTCATCCGGATAAAGAGTTAGCGAGGGCTTTGCACGAAGCTCAACCGAGCATCTATAAAGATGACAATCACAAGCCAGAAATGGCTTTAGCATTGACTGGGTTTGAGGCTCTTTGTGGGTTCATCAGTTCTAAGGTACAACAATATAGCAGCAAAGGGTAGTTCTTCTGGGGCTTTGAATTTTTTCTTAAAACTTCTTTACAGCACAAATATTCTTGTTTTTCCTGATGGTTAAGTGAAAACATCTTAATGATTCTTACTCTGTTCTTGTTACGCAAAATGAATGAAAATTCTTTATAAATTCCTTTGGTTGTTCTTATTTCATTATATTTTCAGGAGCTCAAGGGGGTGCTTAGTAGCGTTCCTGAGATAGTAGAATTAGTCCAAGGTGCAGATGCAGAGAAGTTCTCACACGATAGTGAGCAAAATGGGAAGGAGAAAGTGAAATCACTTTTTGAGTCCATATTTGGTCAGATCATGTCATCTAACAAGGCCATGGTTCGTGAGGCTATATGTAAACTAAAAAGGCGACTTAGTTTGGAGAAAAAGGTAACTGAACTTATATATCTCTGCAACTTATCTTCTTAGCAGGACTTGTTATGTTTTATTCTCAAGACTTGTTATGTTAAAACATTAAGCATGTAGAAGGTTTCAGTCTCTAATACTGTATGCTTATGCAAATTATTGATGGAAGAAAAGCAGCTAATCTGAATTTAAAGGTGGCTGGTGAATTCTCTTACATATCATTATATAATAAGTTTTTTTTTCTTTTAGTTCAACAATATTTGGGGTGGGGGATTCGAAGCAAGTGCAGCATTGCTCTTCGATTAAAATTTACACTTAGTAGCAGGACATTGATTTACATATTTTAGCATTGTGGGGATCTTATAATGTAGGGCAGATCTAAAACTTGAACAACCAATAGGACCGATTAACTGCTCAATACCTTCCGATAATCATCCAACAAATATTTAGCTTTTAGCATGACAAAGTTTTAGGTAAGAAAAATTAGAAATGCATAGCTGAGTGTATTAAATTATTCTAGAATCTAAACCATGTGGAGTCAATAAATCAGTTTATTAATATTCACAATTTTCTGATTGTTTATTGCTTGGAGAACATTGAATATTATAAACCAATTGTGATATGATTCCTGGGAGAATGTAAACAGGCAAACAGAATTGATGAACCAATGAATAGATGGTCTATCTAGAGCATTGTAGAGTATCCCATGTTTGCCAATCTGAGCAGTGCATTCTTGGTAATTAGTAGGATATACGAACCTCTTTAAGCCTTTTTCTAATAGATGGTGACCGCTCAGGAGCAAGATCACATTCTATTTAAAGTTATTTCAGTGAATATCGATCATCATTAGCAAGTTCTGTGATTTATTTAGCTTAACCTGTTCTTCCTTAGCTTCATCAAACTCTGCAAAGCGTTGTTTGTTTGTGTTCAATTTTTTTATTACTTTTTGCGAATTCTCATATCATTACATTTTTTTTTTTTGATCAGAAGAGGCAACTGTCTACCAAAGAACAATTGGTTCTCAGACTAGAAGCTCAATATCCTGCTGATGTCGGTATACTAGCAGCATTTTTTCTAAACTATGTGAAGTTAAAACCAGGCGAAGCGTTATATCTTGGTCCGAATGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAATGTTGTTCGTGCCGGCCTTACCTCTAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGTATCACAGAATTTATACATGTGCTGGTGTTTTATAATGCTTCGATTCTAGCATATTGGCTCTTGTTTTCCATGTCCTCATTTCTTAAGGCCCCTCTACAGTCATACTTCTTTTTTACCTGTTCTACTGCTTACCAGCAGAAAGTAGTGAAAAACGCCTCAATAGTACGATCTCGAAGGACTCCATCCATATTAATAATACATCATTGTTATCCATTGAGAGATAGGGGAAGCTAGTGGGAATACTAATCTGGTTTTGAAACAAAATTTGACTCATTCTTCTTGGTTTTTACCTCCTCTAAGCTGCCCTGCCCTGCGCCTTAGAGCTTCAATGACAATATTTCTCATAATCAGTTCCTGCAACGTGCAGGGGTTTCCCGAAATCTTGCAAGGAGTTTCGTTGAATGAATACACACGAAAGTACGCCCCTCCTTTTGATGAATTTGAAGTTGATCGCTGTATTCTTCCAGTAGCTGCATCTGTGTCCTTCCCCTCTGTCCCAGGACCATCTCTGTTTCTGTTAATGTCTGGAAAAGGGACAATTGTGACAGGCTATTCTGAAGAAAGTACTTTTCAAGAGGGTGAGGTTCTGTTTGTTCCAGCATATATGGAGGTCAGTGTAACAGCTGCAGCTACAGAATTACATATGTACAGGGCTGGGGTCAACAACAGATTCTTTAGAGACTTGTGATTTACAAGTACAAGTGAATAAATTAAGAATGTACAAGGAAAATAGAGGTTACAAGAAGAATTGTTCAAGCACACACAAATTTGTTGCATTTACTGCCCTTTATGAGTTCATTTTTATGGATTAAATTGCATCAAAGTTGTGCCATATACATTTGTTTAAGAAGAATATGAATAGGTTATCAAGTTGTGTTAAAGTTCATGATAGCGTTGAGCAAAATATTGCTTCATTTATTGTTGTCCTATCAGTTTATCAGCTGTCAACGGTGAATATTCATAAGAGTTCTTTGTTTGGGAGTATTAAATTTAAGGCTCCCTTCCTATCCTCGTTTCAAGAGAAGTCAAGAGGCCACAGGTTTTGATGTAGGGATTGTTTTTAAAATAATATATTACTATTTTGGTTAAGGCCATTTGGTTGTGTTTTGATTTTTTTTTGATAAATTGATGGGTTTTGGTAGGGG

mRNA sequence

CTGACTACCTCCAGCTATGCTAACCGCTCTCTCCTTCTCTATCTTTCTCTGCTTATATAAGTTATAACGAACACAGGATCCTGAACCTTCTTCAATTCCCAAGAGACTCTCAGTTTCTTCTTCTGAGGCTTTTGATTTTTTTATTTTTGATTTACGTCCATTTTTGTTGTTCTTGGTGATGAGCAATGGCCGAAGAAGATGATAAGAGATTGAAGAAAAGGAAGAAGAAGAGGTTGATAGGCGCCGACAGGAGGAGGCGTCTCCTTCGTCTCAAATGCTGTGTGCAGAACTACGATTGGGGGGTTCTAGGGTGTAATTCACAAGTTGCGAGGATGTTTTTGCTCAATTCGGGCTCCTACAGTGCCGATCCGGATGAATGTTACGCGGAGTTCTGGATTGGAACGCATAAATCGGGTCCGTCTTTCGTTTTTTTTGGACGGGACTATAATGCCGCTGCAATTGGATCTAAACCCTTGAGTCTCAAGGACTGGATCACGGTCGATCCGGGCGCTGTGCTTGGTGATAAGGTTGCGCGGAAATGGGGAGGCGATCTCCCCTTCCTCTTCAAGGTGCTGTCGGTAGAGAAGGCTTTATCTATTCAGGCTCATCCGGATAAAGAGTTAGCGAGGGCTTTGCACGAAGCTCAACCGAGCATCTATAAAGATGACAATCACAAGCCAGAAATGGCTTTAGCATTGACTGGGTTTGAGGCTCTTTGTGGGTTCATCAGTTCTAAGGAGCTCAAGGGGGTGCTTAGTAGCGTTCCTGAGATAGTAGAATTAGTCCAAGGTGCAGATGCAGAGAAGTTCTCACACGATAGTGAGCAAAATGGGAAGGAGAAAGTGAAATCACTTTTTGAGTCCATATTTGGTCAGATCATGTCATCTAACAAGGCCATGGTTCGTGAGGCTATATGTAAACTAAAAAGGCGACTTAGTTTGGAGAAAAAGAAGAGGCAACTGTCTACCAAAGAACAATTGGTTCTCAGACTAGAAGCTCAATATCCTGCTGATGTCGGTATACTAGCAGCATTTTTTCTAAACTATGTGAAGTTAAAACCAGGCGAAGCGTTATATCTTGGTCCGAATGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAATGTTGTTCGTGCCGGCCTTACCTCTAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGGGTTTCCCGAAATCTTGCAAGGAGTTTCGTTGAATGAATACACACGAAAGTACGCCCCTCCTTTTGATGAATTTGAAGTTGATCGCTGTATTCTTCCAGTAGCTGCATCTGTGTCCTTCCCCTCTGTCCCAGGACCATCTCTGTTTCTGTTAATGTCTGGAAAAGGGACAATTGTGACAGGCTATTCTGAAGAAAGTACTTTTCAAGAGGGTGAGGTTCTGTTTGTTCCAGCATATATGGAGGTCAGTGTAACAGCTGCAGCTACAGAATTACATATGTACAGGGCTGGGGTCAACAACAGATTCTTTAGAGACTTGTGATTTACAAGTACAAGTGAATAAATTAAGAATGTACAAGGAAAATAGAGGTTACAAGAAGAATTGTTCAAGCACACACAAATTTGTTGCATTTACTGCCCTTTATGAGTTCATTTTTATGGATTAAATTGCATCAAAGTTGTGCCATATACATTTGTTTAAGAAGAATATGAATAGGTTATCAAGTTGTGTTAAAGTTCATGATAGCGTTGAGCAAAATATTGCTTCATTTATTGTTGTCCTATCAGTTTATCAGCTGTCAACGGTGAATATTCATAAGAGTTCTTTGTTTGGGAGTATTAAATTTAAGGCTCCCTTCCTATCCTCGTTTCAAGAGAAGTCAAGAGGCCACAGGTTTTGATGTAGGGATTGTTTTTAAAATAATATATTACTATTTTGGTTAAGGCCATTTGGTTGTGTTTTGATTTTTTTTTGATAAATTGATGGGTTTTGGTAGGGG

Coding sequence (CDS)

ATGGCCGAAGAAGATGATAAGAGATTGAAGAAAAGGAAGAAGAAGAGGTTGATAGGCGCCGACAGGAGGAGGCGTCTCCTTCGTCTCAAATGCTGTGTGCAGAACTACGATTGGGGGGTTCTAGGGTGTAATTCACAAGTTGCGAGGATGTTTTTGCTCAATTCGGGCTCCTACAGTGCCGATCCGGATGAATGTTACGCGGAGTTCTGGATTGGAACGCATAAATCGGGTCCGTCTTTCGTTTTTTTTGGACGGGACTATAATGCCGCTGCAATTGGATCTAAACCCTTGAGTCTCAAGGACTGGATCACGGTCGATCCGGGCGCTGTGCTTGGTGATAAGGTTGCGCGGAAATGGGGAGGCGATCTCCCCTTCCTCTTCAAGGTGCTGTCGGTAGAGAAGGCTTTATCTATTCAGGCTCATCCGGATAAAGAGTTAGCGAGGGCTTTGCACGAAGCTCAACCGAGCATCTATAAAGATGACAATCACAAGCCAGAAATGGCTTTAGCATTGACTGGGTTTGAGGCTCTTTGTGGGTTCATCAGTTCTAAGGAGCTCAAGGGGGTGCTTAGTAGCGTTCCTGAGATAGTAGAATTAGTCCAAGGTGCAGATGCAGAGAAGTTCTCACACGATAGTGAGCAAAATGGGAAGGAGAAAGTGAAATCACTTTTTGAGTCCATATTTGGTCAGATCATGTCATCTAACAAGGCCATGGTTCGTGAGGCTATATGTAAACTAAAAAGGCGACTTAGTTTGGAGAAAAAGAAGAGGCAACTGTCTACCAAAGAACAATTGGTTCTCAGACTAGAAGCTCAATATCCTGCTGATGTCGGTATACTAGCAGCATTTTTTCTAAACTATGTGAAGTTAAAACCAGGCGAAGCGTTATATCTTGGTCCGAATGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAATGTTGTTCGTGCCGGCCTTACCTCTAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGGGTTTCCCGAAATCTTGCAAGGAGTTTCGTTGAATGAATACACACGAAAGTACGCCCCTCCTTTTGATGAATTTGAAGTTGATCGCTGTATTCTTCCAGTAGCTGCATCTGTGTCCTTCCCCTCTGTCCCAGGACCATCTCTGTTTCTGTTAATGTCTGGAAAAGGGACAATTGTGACAGGCTATTCTGAAGAAAGTACTTTTCAAGAGGGTGAGGTTCTGTTTGTTCCAGCATATATGGAGGTCAGTGTAACAGCTGCAGCTACAGAATTACATATGTACAGGGCTGGGGTCAACAACAGATTCTTTAGAGACTTGTGA

Protein sequence

MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSADPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVREAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFVPAYMEVSVTAAATELHMYRAGVNNRFFRDL
Homology
BLAST of CmaCh04G020660 vs. ExPASy Swiss-Prot
Match: Q9M884 (Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.9e-128
Identity = 237/424 (55.90%), Postives = 311/424 (73.35%), Query Frame = 0

Query: 23  RRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSADPDECYAEFWIGTHKSGPSFVF 82
           RRRL RL+C V++YDWG +G +S V R++  NS  Y  DP   YAE W+GTH+SGPS   
Sbjct: 17  RRRLRRLRCSVKDYDWGKIGSDSLVYRVYAANS-DYEIDPTRPYAELWMGTHESGPS--- 76

Query: 83  FGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQAHP 142
               Y   A GS  ++L+ WIT +P + LG++V  KWG DLPFLFKVLSV + LSIQAHP
Sbjct: 77  ----YLEDADGSNGVTLRSWITENPKS-LGNRVLEKWGCDLPFLFKVLSVARPLSIQAHP 136

Query: 143 DKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQG 202
           DK+LA+ +H+A P++YKDDNHKPEMALA T FEALCGFI  +ELK V+ ++PEI ELV  
Sbjct: 137 DKKLAKKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEELVGS 196

Query: 203 ADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVREAICKLKRRLSLEKKKRQLSTK 262
            +A +    +E + +EKVKS+  +IF  +MS++    ++ + KLKRRL +E ++RQL+ K
Sbjct: 197 EEANQVFCITEHD-EEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQLTDK 256

Query: 263 EQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGPNEPHAYISGECIECMATSDNVV 322
           E+LVL+LE QYP D+G+++AFF NYVKL PGEALYLG NEPHAY+ GEC+E MATSDNVV
Sbjct: 257 ERLVLKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATSDNVV 316

Query: 323 RAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPVAASV 382
           RAGLTSK  D+Q L SML+YK G+PEIL+G  +  Y  +Y PPF+EFEVD C LP  AS 
Sbjct: 317 RAGLTSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPSGAST 376

Query: 383 SFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFVPAYMEVSVTAAATELHMYRAGV 442
            FPSVPGPSL L++ G+G + T  S +     G+VLFVPA  E+ +  ++++L +YRAG+
Sbjct: 377 VFPSVPGPSLLLVLQGEGRMSTEASADG-ISMGDVLFVPADTEIHL-RSSSDLKLYRAGI 428

Query: 443 NNRF 447
           N+RF
Sbjct: 437 NSRF 428

BLAST of CmaCh04G020660 vs. ExPASy Swiss-Prot
Match: Q9FZH5 (Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 7.3e-128
Identity = 233/423 (55.08%), Postives = 302/423 (71.39%), Query Frame = 0

Query: 28  RLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSADPDECYAEFWIGTHKSGPSFVFFGRDY 87
           RL+C V+NY+WG LG  S VAR+   N+G    D +  YAEFW+GTH+SGPS V FG  +
Sbjct: 24  RLRCFVKNYEWGKLGPESLVARLQEANTGQ-RVDSEIPYAEFWMGTHESGPSHVEFGSGH 83

Query: 88  NAAAIGSKPLSLKDWITVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQAHPDKELA 147
             +      ++LK W+  +P  +LG KV  KWG DLPFLFKVLSV KALSIQAHP+K LA
Sbjct: 84  GVS--DKCMVTLKSWVLDNPN-LLGSKVVDKWGCDLPFLFKVLSVTKALSIQAHPNKALA 143

Query: 148 RALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQGADAEK 207
             LH   P +Y+D+NHKPE+ALA+T F+ALCGF++ KELK V+++VPEI ELV    A++
Sbjct: 144 EKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVGSKAADQ 203

Query: 208 FSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVREAICKLKRRLSLEKKKRQLSTKEQLVL 267
             +  E +  E++KS+   IF Q+MS++    ++ + ++K RL LE K R+LS KE+LVL
Sbjct: 204 IFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSEKEKLVL 263

Query: 268 RLEAQYPADVGILAAFFLNYVKLKPGEALYLGPNEPHAYISGECIECMATSDNVVRAGLT 327
            LE QY  D+G+++AFF NYVKL PGEALYL  NEPHAYISG+C+ECMA SDNVVRAGLT
Sbjct: 264 ELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNVVRAGLT 323

Query: 328 SKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPVAASVSFPSV 387
            K RDVQ L SML YK G+PEIL+G  L  Y  +Y PPFDEFEVD C LP   S  FP+V
Sbjct: 324 PKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKSTVFPAV 383

Query: 388 PGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFVPAYMEVSVTAAATELHMYRAGVNNRFF 447
           PGPS++L++ GKG + TG S +     G+VLFVPA +E+ VT  +  + +YRAGV++RFF
Sbjct: 384 PGPSVYLVIEGKGQLRTG-SSKVLVNRGDVLFVPADIEIHVTGESDVMKLYRAGVSSRFF 441

Query: 448 RDL 451
           + L
Sbjct: 444 QTL 441

BLAST of CmaCh04G020660 vs. ExPASy Swiss-Prot
Match: Q9HFU4 (Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=MAN1 PE=3 SV=2)

HSP 1 Score: 280.4 bits (716), Expect = 3.6e-74
Identity = 179/436 (41.06%), Postives = 238/436 (54.59%), Query Frame = 0

Query: 33  VQNYDWGVLGCNSQVARMFLLNSGSYSADPDECYAEFWIGTHKSGPSFVFFGRDYNAAAI 92
           + +YDWG  G  S  A++   +   +S D D+ YAE W+GTH + PS +           
Sbjct: 12  INSYDWGKKGSASLAAQLATTSIPDFSIDEDKAYAELWMGTHPNNPSRL----------- 71

Query: 93  GSKPLSLKDWITVDPGAVLGDKVARKW----GGDLPFLFKVLSVEKALSIQAHPDKELAR 152
            S    L + +   P  ++G  V+ K+     G LPFLFKVLS+  ALSIQAHPDK LA+
Sbjct: 72  -SDNTLLSEHLKSHP-ELIGSSVSSKFEDCKDGSLPFLFKVLSIGTALSIQAHPDKPLAK 131

Query: 153 ALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQGADAEKF 212
            L + +P +YKD NHKPEMA+ALT F A   F+    L   L +VPE+ E V  +  E  
Sbjct: 132 KLFDEKPDVYKDPNHKPEMAIALTPFLAFLNFLPLSVLLLHLLTVPELQEFVDSSLTESL 191

Query: 213 SHD----------------SEQNGKEKVKSLFESIFGQIMSSNKAMVREAICKLKRRLSL 272
           +                  +E     + K + + IF  +MS++K +V EAI KL +R   
Sbjct: 192 ASSLGLPTSQPPDTSLFKPTESPATAEQKDILKQIFAALMSADKKLVEEAISKLIKRYQA 251

Query: 273 EKK-KRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGPNEPHAYISGEC 332
           ++  K    +   L LRL  QYP DVG+L  F LN V+LK GEA +LG NEPHAYI G+ 
Sbjct: 252 KRDIKENEKSLVDLALRLNDQYPGDVGVLCVFLLNVVELKRGEAAFLGANEPHAYIEGDI 311

Query: 333 IECMATSDNVVRAGLTSKKRDVQALLSMLNYK--QGFPEILQGVSL---NEYTRKYAPPF 392
           IECMATSDNVVRAGLT K RDV  L+SML Y+   G  ++LQ       ++ T+ Y PP 
Sbjct: 312 IECMATSDNVVRAGLTPKLRDVDTLVSMLTYEAAPGNKQLLQPTPFQKGDDTTKLYDPPI 371

Query: 393 DEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFVPAYMEV 443
            EF V R  L      S   V GPSL ++  G+G +  G ++ S F  G+V+FV A  EV
Sbjct: 372 AEFSVLRTELSKGMKTSHRPVEGPSLCVITEGEGVVRNG-NDRSEFVRGDVIFVGAGKEV 431

BLAST of CmaCh04G020660 vs. ExPASy Swiss-Prot
Match: Q9GP38 (Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis OX=6211 GN=PMIH PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.3e-73
Identity = 179/453 (39.51%), Postives = 250/453 (55.19%), Query Frame = 0

Query: 26  LLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSADPDECYAEFWIGTHKSGPSFVFFGR 85
           +LRLKC  Q YDWG +G NS V ++ + +     +  D  YAE W+GTH SG S ++   
Sbjct: 1   MLRLKCASQRYDWGKVGVNSIVYKLQVASENPDISHVDLPYAELWMGTHPSGSSCLWDNP 60

Query: 86  DYNAAA-IGSKPLSLKDWITVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQAHPDK 145
               AA I   P S            LG      +G  LPFLFKVLSV KALSIQAHPDK
Sbjct: 61  HVTLAAHIQENPAS------------LGKPSLIYFGRRLPFLFKVLSVAKALSIQAHPDK 120

Query: 146 ELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVEL----- 205
           ++A  LH  QP +YKD +HKPEMA+ALT FEAL GF    ++   + + PE+ EL     
Sbjct: 121 KMAVRLHAEQPDLYKDGSHKPEMAIALTDFEALLGFRPLNQILAFIQAFPELAELTTLEL 180

Query: 206 --------VQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVREAICKLKRRLS 265
                   VQ    ++   +  ++  EKV+S  +S+  +  + +K++    +      L 
Sbjct: 181 PSPEEKRDVQPPSIKQLYSNLMRSSPEKVESTIKSLLNRFTTGSKSVCDPPL----SILG 240

Query: 266 LEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGPNEPHAYISGEC 325
           ++ ++  +     L LRL   +P DVG L+ FFLNY++LK GEA++L  N PHAY+SG+C
Sbjct: 241 VDLQEEDVQALVDLFLRLTQAFPGDVGCLSIFFLNYIRLKSGEAIFLKANTPHAYLSGDC 300

Query: 326 IECMATSDNVVRAGLTSKKRDVQALLSMLNY-------KQGFPEIL---QGVSLNEYTRK 385
           +ECMA SDNVVRAGLT K +DV+ LL ML+Y       + G  + +   +G+S+    + 
Sbjct: 301 VECMANSDNVVRAGLTPKFKDVERLLEMLDYTPLTDSLRLGATQPIPTPEGISM----KS 360

Query: 386 YAPPFDEFEVDRCILPV-AASVSFPSVPGPSLFLLMSGKGTIV---TG--YSEESTFQEG 445
           + PP  EF VD       +   S PSVP  S+ L + G+GTI    TG   S+E+ F  G
Sbjct: 361 FIPPVSEFAVDVIQFDAESRGFSLPSVPTASILLFLHGQGTITCPSTGEACSQETKFGPG 420

BLAST of CmaCh04G020660 vs. ExPASy Swiss-Prot
Match: Q54PA0 (Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=mpi PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.0e-73
Identity = 174/454 (38.33%), Postives = 254/454 (55.95%), Query Frame = 0

Query: 21  DRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSADPDEC--YAEFWIGTHKSGP 80
           +    ++ LKC  QNY+WG  G NS VA++    +   S    E   YAE W+G H S P
Sbjct: 16  NNNNNIVLLKCVSQNYEWGKYGSNSTVAKLLKGYAKECSDIIKETIPYAELWMGDHVSAP 75

Query: 81  SFVFFGRDYNAAAIGSKPLSLKDWI--------------TVDPGAVLGDKVARKWGGDLP 140
           S V +          +K L L+++I              +    ++ G+ V +++G D P
Sbjct: 76  SKVEY---------KNKELKLREYIDTVQKEINEKSSTSSSSSSSIRGEIVEKRFGNDFP 135

Query: 141 FLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSK 200
           FLFKVLS+  ALSIQAHPD +LA+ L +  P+IYKD  HKPE+A+A T FEALC F    
Sbjct: 136 FLFKVLSIRTALSIQAHPDSQLAQVLFKKYPNIYKDPYHKPEIAIATTPFEALCSFRPLS 195

Query: 201 ELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVREAIC 260
           E++  + ++PE           K S  +     +  K   +SI   ++ ++  ++   + 
Sbjct: 196 EIQSFIDTIPEF----------KNSLPNNLIKLDDCKEYLKSIVTSLLKADGLLISNNLK 255

Query: 261 KLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGPNEPH 320
           +L  RL+ EK++ +    ++LVL+L +QYP DVG+  A+ LNY+ LKPGEAL+LG  EPH
Sbjct: 256 ELNNRLN-EKREEERDDLDRLVLKLYSQYPGDVGVFFAYILNYIVLKPGEALFLGAGEPH 315

Query: 321 AYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL-----QGVSLNEYT 380
           AYISG+C+ECMA SDNVVRAGLT K +DV  L  ML Y+ G P+++       +SL  Y 
Sbjct: 316 AYISGDCVECMAPSDNVVRAGLTPKLKDVDTLGDMLTYRTGRPDLVVPQQRPDLSLTNY- 375

Query: 381 RKYAPPFDEFEVDRCIL-PVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQE---G 440
           R Y PP DEF+V+   L     +++  S  GPS+ L+ SG  +I    S +ST      G
Sbjct: 376 RCYQPPVDEFQVEYYHLDDCCKNINVYSSKGPSIVLVYSGNLSI-ENKSNQSTLNNLHTG 435

Query: 441 EVLFVPAYMEVSV--TAAATELHMYRAGVNNRFF 448
            +LFVPA  +     + ++  + +Y A V+NR F
Sbjct: 436 SILFVPANTDYQFIQSDSSIPVSIYVASVSNRIF 447

BLAST of CmaCh04G020660 vs. ExPASy TrEMBL
Match: A0A6J1I443 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111470445 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 9.5e-256
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120
           DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120

Query: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180
           GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF
Sbjct: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180

Query: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR 240
           ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR
Sbjct: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR 240

Query: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300
           EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP
Sbjct: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300

Query: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360
           NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR
Sbjct: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360

Query: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV 420
           KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV
Sbjct: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV 420

Query: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 451
           PAYMEVSVTAAATELHMYRAGVNNRFFRDL
Sbjct: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 450

BLAST of CmaCh04G020660 vs. ExPASy TrEMBL
Match: A0A6J1E4R0 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111429906 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 9.8e-253
Identity = 444/450 (98.67%), Postives = 447/450 (99.33%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           MAEEDDKRLKKRKKKRLIGADRR+RL+RLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRKRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120
           DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120

Query: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180
           GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF
Sbjct: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180

Query: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR 240
           ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIF QIMSSNKAMVR
Sbjct: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAMVR 240

Query: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300
           EAICKLKRRLSLEKKKRQLS KEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP
Sbjct: 241 EAICKLKRRLSLEKKKRQLSAKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300

Query: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360
           NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR
Sbjct: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360

Query: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV 420
           KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEE TFQEGEVLFV
Sbjct: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEEITFQEGEVLFV 420

Query: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 451
           PAYMEVSVTAAATELHMYRAGVNNRFF+DL
Sbjct: 421 PAYMEVSVTAAATELHMYRAGVNNRFFKDL 450

BLAST of CmaCh04G020660 vs. ExPASy TrEMBL
Match: A0A6J1FJN3 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444656 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.1e-231
Identity = 407/452 (90.04%), Postives = 427/452 (94.47%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           M EEDD RLKKRKKKRL+GADRR+RLLRLKC VQNYDWGV+GCNSQVAR+F LNSGS + 
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYN--AAAIGSKPLSLKDWITVDPGAVLGDKVARK 120
           DP ECYAEFWIGTHKSGPSFV FGRDYN  AAA+GS  +SLKDWI+VDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIY+DDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYEDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAM 240
           GFISSKELKGVLSSVPEIVELVQ  D EKFSHDS Q+GKEKVK LFESIF QIMSSNKA+
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDTEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKAI 240

Query: 241 VREAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYL 300
           VREAICKLKRRLS EKKKRQLS KEQL+LRLE QYPADVGILAAFFLNYVKLKPGEALY+
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRD+QALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDIQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVL 420
           TRKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL++SGKGTI+TGYSEEST QEGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSVTAAATELHMYRAGVNNRFFRDL 451
           FVPAYMEVS+T+ ATELH+YRAGVNNRFF DL
Sbjct: 421 FVPAYMEVSITSGATELHVYRAGVNNRFFGDL 452

BLAST of CmaCh04G020660 vs. ExPASy TrEMBL
Match: A0A6J1K047 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111489838 PE=3 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 5.8e-229
Identity = 404/451 (89.58%), Postives = 424/451 (94.01%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           M EEDD RLKKRKKKRL+GADRR+RLLRLKC VQNYDWGV+GCNSQVAR+F LNSGS + 
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYN--AAAIGSKPLSLKDWITVDPGAVLGDKVARK 120
           DP ECYAEFWIGTHKSGPSFV FGRDYN  AAA+GS  +SLKDWI+VDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGG+LPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC
Sbjct: 121 WGGELPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAM 240
           GFISSKELKGVLSSVPEIVELVQ  DAEKFSHDS Q+GKEKVK LFESIF QIMSSNK +
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYL 300
           VR+AICKLKRRLS EKKKRQLS KEQL+LRLE QYPADVGILAAFFLNYVKLKPGEALY+
Sbjct: 241 VRDAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVL 420
           TRKYAPPFDEFEVDRCILP AASVSF SVPGPSLFL++SGKGTI+TGYSEEST QEGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFASVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSVTAAATELHMYRAGVNNRFFRD 450
           FVPAYMEVS+T+ ATEL +YRAGVNNRFF D
Sbjct: 421 FVPAYMEVSITSGATELRLYRAGVNNRFFGD 451

BLAST of CmaCh04G020660 vs. ExPASy TrEMBL
Match: A0A0A0KL16 (Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_5G167200 PE=3 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.4e-227
Identity = 399/451 (88.47%), Postives = 426/451 (94.46%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           M EEDDK+L+K +KKRL GADRR+RLLRLKCCVQNYDWGV+GCNSQVAR+F LNSG  + 
Sbjct: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNA-AAIGSKPLSLKDWITVDPGAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFV +GRD NA AA GSKPLSLKD I+VDP AVLGDKVARKW
Sbjct: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLS+EKALSIQAHPDK+LAR+L+EAQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180

Query: 181 FISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMV 240
           FISSKELK VLSSVPEIVELVQ ADAEKFSHDSEQ+GKEKVK LFESIF QIMSSNK +V
Sbjct: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240

Query: 241 REAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLG 300
           REA+CKLKRRLSLEKKKRQLS KEQL+LRLE+QYPADVGILAA+FLNYV+LKPGEALY+G
Sbjct: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLF 420
           RKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL+MSGKGTI+TGYSEE+T QEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420

Query: 421 VPAYMEVSVTAAATELHMYRAGVNNRFFRDL 451
           VPAYMEVS+TA A ELHMYRAG+NNRFFRDL
Sbjct: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451

BLAST of CmaCh04G020660 vs. NCBI nr
Match: XP_022971761.1 (mannose-6-phosphate isomerase 1-like [Cucurbita maxima])

HSP 1 Score: 892.1 bits (2304), Expect = 2.0e-255
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120
           DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120

Query: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180
           GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF
Sbjct: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180

Query: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR 240
           ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR
Sbjct: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR 240

Query: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300
           EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP
Sbjct: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300

Query: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360
           NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR
Sbjct: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360

Query: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV 420
           KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV
Sbjct: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV 420

Query: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 451
           PAYMEVSVTAAATELHMYRAGVNNRFFRDL
Sbjct: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 450

BLAST of CmaCh04G020660 vs. NCBI nr
Match: XP_023511752.1 (mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 884.0 bits (2283), Expect = 5.4e-253
Identity = 446/450 (99.11%), Postives = 448/450 (99.56%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           MAEEDDKRLKKRKKKRLIGADRRRRL+RLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRRRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120
           DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120

Query: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180
           GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF
Sbjct: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180

Query: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR 240
           ISSKELKGVLSSVPEIVELVQ ADAEKFSHDSEQNGKEKVKSLFESIF QIMSSNKAMVR
Sbjct: 181 ISSKELKGVLSSVPEIVELVQVADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAMVR 240

Query: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300
           EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP
Sbjct: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300

Query: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360
           NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR
Sbjct: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360

Query: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV 420
           KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEE+TFQEGEVLFV
Sbjct: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEETTFQEGEVLFV 420

Query: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 451
           PAYMEVSVTAAATELHMYRAGVNNRFFRDL
Sbjct: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 450

BLAST of CmaCh04G020660 vs. NCBI nr
Match: XP_022921753.1 (mannose-6-phosphate isomerase 1-like [Cucurbita moschata])

HSP 1 Score: 882.1 bits (2278), Expect = 2.0e-252
Identity = 444/450 (98.67%), Postives = 447/450 (99.33%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           MAEEDDKRLKKRKKKRLIGADRR+RL+RLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRKRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120
           DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120

Query: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180
           GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF
Sbjct: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180

Query: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR 240
           ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIF QIMSSNKAMVR
Sbjct: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAMVR 240

Query: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300
           EAICKLKRRLSLEKKKRQLS KEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP
Sbjct: 241 EAICKLKRRLSLEKKKRQLSAKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300

Query: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360
           NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR
Sbjct: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360

Query: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV 420
           KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEE TFQEGEVLFV
Sbjct: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEEITFQEGEVLFV 420

Query: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 451
           PAYMEVSVTAAATELHMYRAGVNNRFF+DL
Sbjct: 421 PAYMEVSVTAAATELHMYRAGVNNRFFKDL 450

BLAST of CmaCh04G020660 vs. NCBI nr
Match: KAG6601810.1 (Mannose-6-phosphate isomerase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 879.8 bits (2272), Expect = 1.0e-251
Identity = 442/450 (98.22%), Postives = 447/450 (99.33%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           MAEEDDKRLKKRKKKRLIGADRR+RL+RLKCCVQNYDWGVLGCNSQVARMFLLNSGSY+A
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRKRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYTA 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120
           DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120

Query: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180
           GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF
Sbjct: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180

Query: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR 240
           ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIF QIMSSNKAMVR
Sbjct: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAMVR 240

Query: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300
           EAICKLKRRLSLEKKKRQLS KEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP
Sbjct: 241 EAICKLKRRLSLEKKKRQLSAKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300

Query: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360
           NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR
Sbjct: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360

Query: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV 420
           KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTI+TGYSEE TFQEGEVLFV
Sbjct: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIMTGYSEEITFQEGEVLFV 420

Query: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 451
           PAYMEVSVTAAATELHMYRAGVNNRFF+DL
Sbjct: 421 PAYMEVSVTAAATELHMYRAGVNNRFFKDL 450

BLAST of CmaCh04G020660 vs. NCBI nr
Match: KAG7032518.1 (Mannose-6-phosphate isomerase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 878.6 bits (2269), Expect = 2.2e-251
Identity = 441/450 (98.00%), Postives = 447/450 (99.33%), Query Frame = 0

Query: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60
           MAEEDDKRLKKRKKKRLIGADRR+RL+RLKCCVQNYDWGVLGCNSQVARMFLLNSGSY+A
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRKRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYTA 60

Query: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120
           DPD+CYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG
Sbjct: 61  DPDDCYAEFWIGTHKSGPSFVFFGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWG 120

Query: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180
           GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF
Sbjct: 121 GDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGF 180

Query: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVR 240
           ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIF QIMSSNKAMVR
Sbjct: 181 ISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAMVR 240

Query: 241 EAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300
           EAICKLKRRLSLEKKKRQLS KEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP
Sbjct: 241 EAICKLKRRLSLEKKKRQLSAKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGP 300

Query: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360
           NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR
Sbjct: 301 NEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTR 360

Query: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFV 420
           KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTI+TGYSEE TFQEGEVLFV
Sbjct: 361 KYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIMTGYSEEITFQEGEVLFV 420

Query: 421 PAYMEVSVTAAATELHMYRAGVNNRFFRDL 451
           PAYMEVSVTAAATELHMYRAGVNNRFF+DL
Sbjct: 421 PAYMEVSVTAAATELHMYRAGVNNRFFKDL 450

BLAST of CmaCh04G020660 vs. TAIR 10
Match: AT3G02570.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 460.7 bits (1184), Expect = 1.4e-129
Identity = 237/424 (55.90%), Postives = 311/424 (73.35%), Query Frame = 0

Query: 23  RRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSADPDECYAEFWIGTHKSGPSFVF 82
           RRRL RL+C V++YDWG +G +S V R++  NS  Y  DP   YAE W+GTH+SGPS   
Sbjct: 17  RRRLRRLRCSVKDYDWGKIGSDSLVYRVYAANS-DYEIDPTRPYAELWMGTHESGPS--- 76

Query: 83  FGRDYNAAAIGSKPLSLKDWITVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQAHP 142
               Y   A GS  ++L+ WIT +P + LG++V  KWG DLPFLFKVLSV + LSIQAHP
Sbjct: 77  ----YLEDADGSNGVTLRSWITENPKS-LGNRVLEKWGCDLPFLFKVLSVARPLSIQAHP 136

Query: 143 DKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQG 202
           DK+LA+ +H+A P++YKDDNHKPEMALA T FEALCGFI  +ELK V+ ++PEI ELV  
Sbjct: 137 DKKLAKKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEELVGS 196

Query: 203 ADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVREAICKLKRRLSLEKKKRQLSTK 262
            +A +    +E + +EKVKS+  +IF  +MS++    ++ + KLKRRL +E ++RQL+ K
Sbjct: 197 EEANQVFCITEHD-EEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQLTDK 256

Query: 263 EQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLGPNEPHAYISGECIECMATSDNVV 322
           E+LVL+LE QYP D+G+++AFF NYVKL PGEALYLG NEPHAY+ GEC+E MATSDNVV
Sbjct: 257 ERLVLKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATSDNVV 316

Query: 323 RAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPVAASV 382
           RAGLTSK  D+Q L SML+YK G+PEIL+G  +  Y  +Y PPF+EFEVD C LP  AS 
Sbjct: 317 RAGLTSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPSGAST 376

Query: 383 SFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFVPAYMEVSVTAAATELHMYRAGV 442
            FPSVPGPSL L++ G+G + T  S +     G+VLFVPA  E+ +  ++++L +YRAG+
Sbjct: 377 VFPSVPGPSLLLVLQGEGRMSTEASADG-ISMGDVLFVPADTEIHL-RSSSDLKLYRAGI 428

Query: 443 NNRF 447
           N+RF
Sbjct: 437 NSRF 428

BLAST of CmaCh04G020660 vs. TAIR 10
Match: AT1G67070.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 458.8 bits (1179), Expect = 5.2e-129
Identity = 233/423 (55.08%), Postives = 302/423 (71.39%), Query Frame = 0

Query: 28  RLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSADPDECYAEFWIGTHKSGPSFVFFGRDY 87
           RL+C V+NY+WG LG  S VAR+   N+G    D +  YAEFW+GTH+SGPS V FG  +
Sbjct: 24  RLRCFVKNYEWGKLGPESLVARLQEANTGQ-RVDSEIPYAEFWMGTHESGPSHVEFGSGH 83

Query: 88  NAAAIGSKPLSLKDWITVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQAHPDKELA 147
             +      ++LK W+  +P  +LG KV  KWG DLPFLFKVLSV KALSIQAHP+K LA
Sbjct: 84  GVS--DKCMVTLKSWVLDNPN-LLGSKVVDKWGCDLPFLFKVLSVTKALSIQAHPNKALA 143

Query: 148 RALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQGADAEK 207
             LH   P +Y+D+NHKPE+ALA+T F+ALCGF++ KELK V+++VPEI ELV    A++
Sbjct: 144 EKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVGSKAADQ 203

Query: 208 FSHDSEQNGKEKVKSLFESIFGQIMSSNKAMVREAICKLKRRLSLEKKKRQLSTKEQLVL 267
             +  E +  E++KS+   IF Q+MS++    ++ + ++K RL LE K R+LS KE+LVL
Sbjct: 204 IFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSEKEKLVL 263

Query: 268 RLEAQYPADVGILAAFFLNYVKLKPGEALYLGPNEPHAYISGECIECMATSDNVVRAGLT 327
            LE QY  D+G+++AFF NYVKL PGEALYL  NEPHAYISG+C+ECMA SDNVVRAGLT
Sbjct: 264 ELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNVVRAGLT 323

Query: 328 SKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPVAASVSFPSV 387
            K RDVQ L SML YK G+PEIL+G  L  Y  +Y PPFDEFEVD C LP   S  FP+V
Sbjct: 324 PKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKSTVFPAV 383

Query: 388 PGPSLFLLMSGKGTIVTGYSEESTFQEGEVLFVPAYMEVSVTAAATELHMYRAGVNNRFF 447
           PGPS++L++ GKG + TG S +     G+VLFVPA +E+ VT  +  + +YRAGV++RFF
Sbjct: 384 PGPSVYLVIEGKGQLRTG-SSKVLVNRGDVLFVPADIEIHVTGESDVMKLYRAGVSSRFF 441

Query: 448 RDL 451
           + L
Sbjct: 444 QTL 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8841.9e-12855.90Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=... [more]
Q9FZH57.3e-12855.08Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=... [more]
Q9HFU43.6e-7441.06Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotyp... [more]
Q9GP382.3e-7339.51Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis OX=6211 GN... [more]
Q54PA03.0e-7338.33Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=m... [more]
Match NameE-valueIdentityDescription
A0A6J1I4439.5e-256100.00Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111470445 PE=3 S... [more]
A0A6J1E4R09.8e-25398.67Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111429906 PE=3... [more]
A0A6J1FJN32.1e-23190.04Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444656 PE=3... [more]
A0A6J1K0475.8e-22989.58Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111489838 PE=3 S... [more]
A0A0A0KL161.4e-22788.47Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_5G167200 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_022971761.12.0e-255100.00mannose-6-phosphate isomerase 1-like [Cucurbita maxima][more]
XP_023511752.15.4e-25399.11mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022921753.12.0e-25298.67mannose-6-phosphate isomerase 1-like [Cucurbita moschata][more]
KAG6601810.11.0e-25198.22Mannose-6-phosphate isomerase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7032518.12.2e-25198.00Mannose-6-phosphate isomerase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT3G02570.11.4e-12955.90Mannose-6-phosphate isomerase, type I [more]
AT1G67070.15.2e-12955.08Mannose-6-phosphate isomerase, type I [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 252..272
NoneNo IPR availableGENE3D1.10.441.10Phosphomannose Isomerase, domain 2coord: 182..348
e-value: 2.4E-132
score: 444.0
NoneNo IPR availablePIRSRPIRSR001480-1PIRSR001480-1coord: 34..441
e-value: 2.6E-125
score: 416.6
NoneNo IPR availablePIRSRPIRSR001480-2PIRSR001480-2coord: 34..441
e-value: 2.6E-125
score: 416.6
NoneNo IPR availablePANTHERPTHR10309:SF10MANNOSE-6-PHOSPHATE ISOMERASEcoord: 17..448
NoneNo IPR availableCDDcd07011cupin_PMI_type_I_Ncoord: 30..342
e-value: 5.1593E-113
score: 330.666
IPR016305Mannose-6-phosphate isomerasePRINTSPR00714MAN6PISMRASEcoord: 285..304
score: 54.0
coord: 304..323
score: 70.0
coord: 65..80
score: 53.12
coord: 323..342
score: 52.0
coord: 29..47
score: 45.26
coord: 158..181
score: 68.33
coord: 269..284
score: 38.12
IPR016305Mannose-6-phosphate isomerasePIRSFPIRSF001480PMIcoord: 23..448
e-value: 2.1E-128
score: 427.1
IPR016305Mannose-6-phosphate isomerasePANTHERPTHR10309MANNOSE-6-PHOSPHATE ISOMERASEcoord: 17..448
IPR001250Mannose-6-phosphate isomerase, type IPFAMPF01238PMI_typeIcoord: 26..405
e-value: 9.2E-102
score: 341.0
IPR001250Mannose-6-phosphate isomerase, type ITIGRFAMTIGR00218TIGR00218coord: 26..433
e-value: 4.0E-74
score: 247.9
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 26..440
e-value: 2.4E-132
score: 444.0
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 30..324
e-value: 2.4E-132
score: 444.0
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00965PMI_I_1coord: 158..166
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 25..443

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G020660.1CmaCh04G020660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000032 cell wall mannoprotein biosynthetic process
biological_process GO:0009298 GDP-mannose biosynthetic process
biological_process GO:0006486 protein glycosylation
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004476 mannose-6-phosphate isomerase activity
molecular_function GO:0008270 zinc ion binding