CmaCh04G020370 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G020370
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Regulator of Vps4 activity in the MVB pathway protein
Location	Cma_Chr04: 13186393 .. 13190921 (+)
RNA-Seq Expression	CmaCh04G020370
Synteny	CmaCh04G020370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCGGTGGATTAGTTGGAAAAGGATGCAGTTGACGACTGTGCCTAGATAAGTCGATCGTCAAAGATCGGCTCTCTAATACCGCCGCTGGGAGAACCGAAGCCTCATTCCATTGAAATAATTCCTCTGATATAGACTGTTCTTACTTCAGTGTTAAGAACGGGAAAGGGGAAAGGAGTATATACAGACATCTGGGTTTCATCTTCCTCGTTCAAGCGTCAATGTCACTCCTCAATCAACTCTTCAACAGAGGCGTCTTTGCGGGCAAATGGTCAGCTTTCGATTTTTCTAATTCTAAACCTCCTCACTATTCCATTAACTATGCTTGAATTTGGTTTCGGATCCTCAGCATTACTTAGAATTCATCTGGGATTGTTATCTTTCCGGGGATTTTTTGTTTGTTGGAGATCTTTTTGTGTCGTTAATTTACCAAACCCAACACCCACAAACGAAATTTCTGTTGAGATTTTGCACAAATTTTCGTTCGGCTAAACTTCGACAAAATAATATATCGACCAGTTTTCCAGTTTTTGTATAATTTCGGCAATTGAATTTTGATTCGTAGTCTTCTACCTTTATCATCTCTCGATTAGACTGGGGTTTTTCCGCACAATTTCATGTCAAAATCTATAATCAAACAAGTACTTGTAAACAGATAGCATCCCTTGCATTTTTCTGGGAATGAAAGGTCTAATATATATTGGGTGTTTTGTAGCAAAACTTGCTTGAGCTTGGCAATCTCGCGGATCAAGCTGCTGCAGAATAAGAGGGATCTACAGCTGAAGAATATGCGCAAGGAGATCGCACAATTCTTGCAGACGGGACAGGAACCAATAGCTCGGATTCGGGTACCCTTTAGAATTTTGAGATCTGTAATGTCTGTCAGTTCTATTGTTTGTTTTACTATCCCGTAAAATAACATGTGTAGTTATCTGTTTTGGCAGGTGGAGCATGTAATACGGGAACAAAACATATGGGCAGCATATGAAATTCTAGAGTTGTTTTGTGAATTTGTTCTTGCTCGTGTCCCCATCATCGAAAGCCAGAAGTAATCTATAGCTCAAAAGTTGTTTTGCGAAATTTTTTTGCTTCTTGGAGTTTCTATTTATATGTTGGTTCACATAAATTGGAACTCCCTTGGTACAATGGATATCTGATGATTCTTAGCATTTGGATCTTTAAATAAGTCATGAAGCTCTTGTTTAACTGGGTCATCTCCATTAATTTTTTGTTTGTCCCTATTAAAAGTTAATTAACTATGAAACTAACTTCTAACTGATATGTGGTTCTGAAGCTTACTTGTGATCCCCAGTCATAACCTTCTCTGTTTGTGTTGGACTTCAATATCGATTGATGCGTCCTTTACATTTTCCGGTGCATCTTTGGGCTGGGTTATGTTGTAGAATTATAAATTATGTGGTGCTTTCTTTGGTGCTGATGTCTAATTTAATAGTCTTGAGAAAATCTGTTAGCTGCAAAGTTATTTCAGTTACACTGTTCTAAGCTACGAAAGAACAAAGTGGTACCTGTTCATGCAATCAAAGGAACTAGTGTCGTGTCTATCCACCAAATTTATTCACTCAATTTTCTTGTCAGTACTAAGTTGTAATTGACATAATTTTCAACGGTAGTATGTATTTGTGAGTTCTCTTGGATAATTATGATAGGAACATCAGTAGGAATATTATTAGGGTATAAAGGGTTTATTAGTAATTAGTTAGGAAGATTGTTAGGGTATTTGATTCTAAATAGAGGGAGTAGGAAAAGAGGAAGTTAGACAATTGTTTAGTGGTTCGGGCTTCGGTGGCATCTCAAGAAAGTGGAGGTTCAAAAGTACCTCAAATACTTGGGAGTTGTCTTGCTGTTCTGTTATCTTTCATATTTCAATGTACTTCGGTTCTATCAAATTGTTGTTAAGATTAGTGTTCCAATGCTATGATGTGAAGATGAGTTCTATTAATTTGCTTGGTTTGATTTGATGCATGTGTTTTTCATTATTTGCTTAGAAACCTGTAGCCATGGCTCATCATTAAAAAAATGGTGAGGTTAATCTGTTGAGTCCTTTTCGTGCAGACCATACTAGGGGCCTTTTATGTGAATGTTCTTACTTTGTGCATTTTGTTTGTCCGTAACTAACTATTAAATTTACAATAACAGAGAATGTCCAGTCGAACTGCGGGAGGCTATTGCGAGCATAATATTTGCTGCTCCTAGATGTTCTGACCTTCCAGATCTACTGCAGTTGAAGAATTTGTTTGCTGTCAAATATGGGAAAGAATTTGTCTCTGCTATTTCTGAGCTTCGTCCTGACTCCGGTGTTAATCGATCAGTTGAGTGTGTCTCAAGTTTATCGTGAGCAGTAGTAATGTTTGTTTTTTTTAGAATAAAAGCGAAATGGTTCATTCAATTCTTTGTTTTCATTTCAGATTATTGAAAAGCTTGCAGTGAGTGCGCCTCCTGGAGAACTAAAGCTCAAGGTCATGAAGGAGATTGCAAAAGAACATGGACTCGACTGGGATTCTTCCTCCACTGCTTCTGAATTTAGTAAAACCCACGAAGATCTGCTGGTATGATTCCCAATTTGATTTTGGTGTTGATTGATCTGAAACACATTCATAGTACTATAGCGAAGTTATAAATGACATATATTCTTGCTTCTGGAAACAATTATTCCATTCTACTGACTGCATATTTCTCCATCCTTTCCCTCATATATTGGCATGATATTTCTAACTGCTGTTCCTGAAAAGCCTGAAAGGAACCGAGATTTGAACTACAGAGCTGAATTGGAAAATGCTTGTATTGGAATGAAAATGGTCTAGATTCTATGATTTTTGAGGGATCAAGAGCTCTCCTTTCTCCAGGGGATCCTCTATAAGCTTCGAACAAAACTCTGTGGCCCACCCCTGTTTTCCTATCCGTAACTGTCCTATCCGTAACTGTCCTATCCGTAACTGTCGAACCCTAACAAATTGCTGACGCACGGCTCAAAAACAGTAAGATTAACAAGTTAACTAATTCGTTCACAGTACTAGAGATTACAACTTAATTATGTTGAAAATTTTTGTTCTGTCTGCTGAAAAGAAGAATATCATTATATGAGACCCTCAATCGTGAGTACGTGCATTACGAGAGGTAGTAAGGAAAAGGACCACCAGACAATTAGTTGGTGGAACGCTCGTACCAGTGGCTGGAGGTGAGGACTAGCAATTGGAGGGAAGTATTCTGTCTTGGGAGGGTGACTTTTCTTTTGAATAGAACCGTGTTTTAGGGGCTGAGAGGTTACCAGCCATCTTGAAATTGGCTGGGAGTTTGTTTGGGTTCTTTGGGTTATTATGAACTTTGTATCTGTTTTTTAAATTTAATCAAGTTGTAAGGGAGGCAACTTACACATTATACACCTACTTTTGACTAACTAGTTTGTCAAGTTCAACCTTAAATATTCAATGTTATCAATTTGAACCCTGAACTTTAAATAGATTGCACAATTAGTATCCTCGAGTTCAAAGTTACATTTTTAGAATATTTGTGGCTGGCTATGAGCATTTTGTGACTAAACTAATCTAGTTTTACGAGAATTAAATCTTCATGTTGGGTACTTTTAGCTTGATCTGAGCAACACACTCACACTAAATGAAGTTTATTGTCATGGTAACTTATTCTGTTCTTGATGGTCTATTCTGTGGCCTTTGTCATGGGATATGCAGGGTGGACCAAAGGTAGTACATGGAGCGGCAGCGTCTCAAGCTCCCTCTCAATATAGCTCGTCCAATCCTTCCACTCCTTATGGAGAATCGCCCGTTGTGTCTGTTGTAGAAAAGCAAGAACCTGAATGCCACCATACATTCACTCGAACCAGCAATAGCGCAAGTGATATCAAACCGGGACCTAAGAATTTTGATGTCAAACCAGAAGTCGCTTCTAAATTAGATATAAGACCACCTCCATCTGATGTATTAGAGAAAGCCAGAGCTGCCATTGCTTCAGCCGAACGTGCCACTGCAGCAGCCCGAGTTGCTGCCGAGCTAGCCAATGTCAACCTTGGAGCAATGAAGCTTGAAGGAGGAGGACAAGCTTCTGAGTGCTGAAGAGAAAAAGAAGGTAATTGATACATTGTTTGATACTGGACTGGTGCATAAATTGTGTTTGAAGTTAATCGGGTGTCCAAAGATTGAAGAGTGGAGAGTGGGTCAATCCATAAGTGAAACCAAATCCGTGTAATTAATAGAGTTGCAAGGAAAATTTAATTCCAAGTATTTGTAAAATACTAAAATTCTTTGGTGATTATGGTGAAGTTCTAATGGTGCAGCAGTTTGATATAAATGCAACATTAGAGGAAAATAAGTTTGCTTAATTTAAGATAAGTTGCAGACTGAGGAAGGGTAAAGTATTCGCAATGTTCTTCCTGAAAGTATGAGCATCCATTGTAGAAATACTCATAAGTTTGGTTGAGAAATTGTGGATTGCCATCAGTTTTTGGTTAAAATGAAGCACCGTTGCCACTGTGTTTTATCCCAAGATCAACAGCGC

mRNA sequence

CTTCGGTGGATTAGTTGGAAAAGGATGCAGTTGACGACTGTGCCTAGATAAGTCGATCGTCAAAGATCGGCTCTCTAATACCGCCGCTGGGAGAACCGAAGCCTCATTCCATTGAAATAATTCCTCTGATATAGACTGTTCTTACTTCAGTGTTAAGAACGGGAAAGGGGAAAGGAGTATATACAGACATCTGGGTTTCATCTTCCTCGTTCAAGCGTCAATGTCACTCCTCAATCAACTCTTCAACAGAGGCGTCTTTGCGGGCAAATGCAAAACTTGCTTGAGCTTGGCAATCTCGCGGATCAAGCTGCTGCAGAATAAGAGGGATCTACAGCTGAAGAATATGCGCAAGGAGATCGCACAATTCTTGCAGACGGGACAGGAACCAATAGCTCGGATTCGGGTGGAGCATGTAATACGGGAACAAAACATATGGGCAGCATATGAAATTCTAGAGTTGTTTTGTGAATTTGTTCTTGCTCGTGTCCCCATCATCGAAAGCCAGAAAGAATGTCCAGTCGAACTGCGGGAGGCTATTGCGAGCATAATATTTGCTGCTCCTAGATGTTCTGACCTTCCAGATCTACTGCAGTTGAAGAATTTGTTTGCTGTCAAATATGGGAAAGAATTTGTCTCTGCTATTTCTGAGCTTCGTCCTGACTCCGGTGTTAATCGATCAATTATTGAAAAGCTTGCAGTGAGTGCGCCTCCTGGAGAACTAAAGCTCAAGGTCATGAAGGAGATTGCAAAAGAACATGGACTCGACTGGGATTCTTCCTCCACTGCTTCTGAATTTAGTAAAACCCACGAAGATCTGCTGGGTGGACCAAAGGTAGTACATGGAGCGGCAGCGTCTCAAGCTCCCTCTCAATATAGCTCGTCCAATCCTTCCACTCCTTATGGAGAATCGCCCGTTGTGTCTGTTGTAGAAAAGCAAGAACCTGAATGCCACCATACATTCACTCGAACCAGCAATAGCGCAAGTGATATCAAACCGGGACCTAAGAATTTTGATGTCAAACCAGAAGTCGCTTCTAAATTAGATATAAGACCACCTCCATCTGATGTATTAGAGAAAGCCAGAGCTGCCATTGCTTCAGCCGAACGTGCCACTGCAGCAGCCCGAGTTGCTGCCGAGCTAGCCAATGTCAACCTTGGAGCAATGAAGCTTGAAGGAGGAGGACAAGCTTCTGAGTGCTGAAGAGAAAAAGAAGGTAATTGATACATTGTTTGATACTGGACTGGTGCATAAATTGTGTTTGAAGTTAATCGGGTGTCCAAAGATTGAAGAGTGGAGAGTGGGTCAATCCATAAGTGAAACCAAATCCGTGTAATTAATAGAGTTGCAAGGAAAATTTAATTCCAAGTATTTGTAAAATACTAAAATTCTTTGGTGATTATGGTGAAGTTCTAATGGTGCAGCAGTTTGATATAAATGCAACATTAGAGGAAAATAAGTTTGCTTAATTTAAGATAAGTTGCAGACTGAGGAAGGGTAAAGTATTCGCAATGTTCTTCCTGAAAGTATGAGCATCCATTGTAGAAATACTCATAAGTTTGGTTGAGAAATTGTGGATTGCCATCAGTTTTTGGTTAAAATGAAGCACCGTTGCCACTGTGTTTTATCCCAAGATCAACAGCGC

Coding sequence (CDS)

ATGTCACTCCTCAATCAACTCTTCAACAGAGGCGTCTTTGCGGGCAAATGCAAAACTTGCTTGAGCTTGGCAATCTCGCGGATCAAGCTGCTGCAGAATAAGAGGGATCTACAGCTGAAGAATATGCGCAAGGAGATCGCACAATTCTTGCAGACGGGACAGGAACCAATAGCTCGGATTCGGGTGGAGCATGTAATACGGGAACAAAACATATGGGCAGCATATGAAATTCTAGAGTTGTTTTGTGAATTTGTTCTTGCTCGTGTCCCCATCATCGAAAGCCAGAAAGAATGTCCAGTCGAACTGCGGGAGGCTATTGCGAGCATAATATTTGCTGCTCCTAGATGTTCTGACCTTCCAGATCTACTGCAGTTGAAGAATTTGTTTGCTGTCAAATATGGGAAAGAATTTGTCTCTGCTATTTCTGAGCTTCGTCCTGACTCCGGTGTTAATCGATCAATTATTGAAAAGCTTGCAGTGAGTGCGCCTCCTGGAGAACTAAAGCTCAAGGTCATGAAGGAGATTGCAAAAGAACATGGACTCGACTGGGATTCTTCCTCCACTGCTTCTGAATTTAGTAAAACCCACGAAGATCTGCTGGGTGGACCAAAGGTAGTACATGGAGCGGCAGCGTCTCAAGCTCCCTCTCAATATAGCTCGTCCAATCCTTCCACTCCTTATGGAGAATCGCCCGTTGTGTCTGTTGTAGAAAAGCAAGAACCTGAATGCCACCATACATTCACTCGAACCAGCAATAGCGCAAGTGATATCAAACCGGGACCTAAGAATTTTGATGTCAAACCAGAAGTCGCTTCTAAATTAGATATAAGACCACCTCCATCTGATGTATTAGAGAAAGCCAGAGCTGCCATTGCTTCAGCCGAACGTGCCACTGCAGCAGCCCGAGTTGCTGCCGAGCTAGCCAATGTCAACCTTGGAGCAATGAAGCTTGAAGGAGGAGGACAAGCTTCTGAGTGCTGA

Protein sequence

MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGESPVVSVVEKQEPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC

Homology

[Close]

BLAST of CmaCh04G020370 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-24
Identity = 68/182 (37.36%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G+ A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY KE+   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

[Close]

BLAST of CmaCh04G020370 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.2e-24
Identity = 68/182 (37.36%), Postives = 108/182 (59.34%), Query Frame = 0


Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G  A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY KE+   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

[Close]

BLAST of CmaCh04G020370 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.2e-24
Identity = 68/182 (37.36%), Postives = 108/182 (59.34%), Query Frame = 0


Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G  A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY KE+   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

[Close]

BLAST of CmaCh04G020370 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.2e-24
Identity = 68/182 (37.36%), Postives = 108/182 (59.34%), Query Frame = 0


Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G  A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY KE+   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

[Close]

BLAST of CmaCh04G020370 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.7e-23
Identity = 67/182 (36.81%), Postives = 107/182 (58.79%), Query Frame = 0


Query: 7   LFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARIRVEHVI 66
           +   G  A + +  L L I+R+KLL+ K+    +  RKEIA +L  G++  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 67  REQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRC-SDLPDLLQL 126
           RE  +  A EILEL+C+ +LAR  +I+S KE    L E+++++I+AAPR  S++ +L  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 127 KNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDS 186
            +    KY K +   +        VN  ++ KL+V APP  L  + + EIAK + + ++ 
Sbjct: 121 ADQLCAKYSKGY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 187 SS 188
            S
Sbjct: 181 DS 181

[Close]

BLAST of CmaCh04G020370 vs. ExPASy TrEMBL
Match: A0A6J1I9L3 (IST1 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470489 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 7.8e-175
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGESPVVSVVEKQE 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGESPVVSVVEKQE
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGESPVVSVVEKQE 240

Query: 241 PECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATAA 300
           PECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATAA
Sbjct: 241 PECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATAA 300

Query: 301 ARVAAELANVNLGAMKLEGGGQASEC 327
           ARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 ARVAAELANVNLGAMKLEGGGQASEC 326

[Close]

BLAST of CmaCh04G020370 vs. ExPASy TrEMBL
Match: A0A6J1E6L9 (IST1 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429884 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.5e-167
Identity = 316/327 (96.64%), Postives = 318/327 (97.25%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLK+MRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGES-PVVSVVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQ PSQYSSSNPSTPYG + PVVSVVEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQVPSQYSSSNPSTPYGAAHPVVSVVEKQ 240

Query: 241 EPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATA 300
           EPECHHT TR SNS SDIKPGPKNFDVK E ASKLD RPPPSDVLEKARAAIASAERATA
Sbjct: 241 EPECHHTLTRISNSVSDIKPGPKNFDVKLEFASKLDTRPPPSDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKLEGGGQASEC 327

[Close]

BLAST of CmaCh04G020370 vs. ExPASy TrEMBL
Match: A0A6J1I6R9 (IST1 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470489 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.1e-151
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 42  MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE 101
           MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE
Sbjct: 1   MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE 60

Query: 102 LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS 161
           LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS
Sbjct: 61  LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS 120

Query: 162 APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSS 221
           APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSS
Sbjct: 121 APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSS 180

Query: 222 NPSTPYGESPVVSVVEKQEPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPS 281
           NPSTPYGESPVVSVVEKQEPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPS
Sbjct: 181 NPSTPYGESPVVSVVEKQEPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPS 240

Query: 282 DVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 327
           DVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 241 DVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 285

[Close]

BLAST of CmaCh04G020370 vs. ExPASy TrEMBL
Match: A0A6J1DR13 (IST1 homolog OS=Momordica charantia OX=3673 GN=LOC111022352 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.8e-145
Identity = 280/327 (85.63%), Postives = 299/327 (91.44%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLK+M KEIAQFLQ GQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMHKEIAQFLQAGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQ+ECPVELREAI+SIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQRECPVELREAISSIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAIS+LRPDSGVNRSIIEKL+VSAPPGELKLKVMKEIA+EHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISDLRPDSGVNRSIIEKLSVSAPPGELKLKVMKEIAQEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGESPVVSVVEKQE 240
           L+WDSS+TASEFSKTHEDLLGGPKVVHGAAASQ P Q+ SSN S      PVVS   KQE
Sbjct: 181 LNWDSSNTASEFSKTHEDLLGGPKVVHGAAASQTPLQHGSSNSSGT--ARPVVSPTGKQE 240

Query: 241 PECHHTFTRTSNSA-SDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATA 300
           P+ HHTF+  +N+  ++IKP  KNF+ KPEV +KLD  PPPSD+LEKARAAIASAERATA
Sbjct: 241 PDRHHTFSPINNTTINEIKPASKNFNFKPEVDAKLDTIPPPSDILEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAEL NVNLGAMKLEGG QAS+C
Sbjct: 301 AARVAAELTNVNLGAMKLEGGAQASDC 325

[Close]

BLAST of CmaCh04G020370 vs. ExPASy TrEMBL
Match: A0A6J1E1A6 (IST1 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429884 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.4e-144
Identity = 276/286 (96.50%), Postives = 277/286 (96.85%), Query Frame = 0


Query: 42  MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE 101
           MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE
Sbjct: 1   MRKEIAQFLQTGQEPIARIRVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVE 60

Query: 102 LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS 161
           LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS
Sbjct: 61  LREAIASIIFAAPRCSDLPDLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVS 120

Query: 162 APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSS 221
           APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQ PSQYSSS
Sbjct: 121 APPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQVPSQYSSS 180

Query: 222 NPSTPYGES-PVVSVVEKQEPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPP 281
           NPSTPYG + PVVSVVEKQEPECHHT TR SNS SDIKPGPKNFDVK E ASKLD RPPP
Sbjct: 181 NPSTPYGAAHPVVSVVEKQEPECHHTLTRISNSVSDIKPGPKNFDVKLEFASKLDTRPPP 240

Query: 282 SDVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 327
           SDVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 241 SDVLEKARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 286

[Close]

BLAST of CmaCh04G020370 vs. NCBI nr
Match: XP_022971809.1 (IST1 homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 622.9 bits (1605), Expect = 1.6e-174
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGESPVVSVVEKQE 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGESPVVSVVEKQE
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGESPVVSVVEKQE 240

Query: 241 PECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATAA 300
           PECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATAA
Sbjct: 241 PECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATAA 300

Query: 301 ARVAAELANVNLGAMKLEGGGQASEC 327
           ARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 ARVAAELANVNLGAMKLEGGGQASEC 326

[Close]

BLAST of CmaCh04G020370 vs. NCBI nr
Match: XP_023538831.1 (IST1 homolog isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602.8 bits (1553), Expect = 1.7e-168
Identity = 319/327 (97.55%), Postives = 320/327 (97.86%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLK+MRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGE-SPVVSVVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYG   PVVS VEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGALHPVVSPVEKQ 240

Query: 241 EPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATA 300
           EPECHHTFTR SNSASDIKPGPKNFDVKPEVASKLD RPPP DVLEKARAAIASAERATA
Sbjct: 241 EPECHHTFTRISNSASDIKPGPKNFDVKPEVASKLDTRPPPWDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKLEGGGQASEC 327

[Close]

BLAST of CmaCh04G020370 vs. NCBI nr
Match: KAG6601778.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 602.4 bits (1552), Expect = 2.3e-168
Identity = 318/327 (97.25%), Postives = 321/327 (98.17%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLK+MRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGES-PVVSVVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYG + PVVSVVEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGTAHPVVSVVEKQ 240

Query: 241 EPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATA 300
           EPECHHTFTR SNSASDIKPGPKN DVKPE ASKLD RPPPSDVLEKARAAIASAERATA
Sbjct: 241 EPECHHTFTRISNSASDIKPGPKNSDVKPEFASKLDTRPPPSDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMK+EGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKVEGGGQASEC 327

[Close]

BLAST of CmaCh04G020370 vs. NCBI nr
Match: XP_022921725.1 (IST1 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 597.4 bits (1539), Expect = 7.3e-167
Identity = 316/327 (96.64%), Postives = 318/327 (97.25%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLK+MRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPYGES-PVVSVVEKQ 240
           LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQ PSQYSSSNPSTPYG + PVVSVVEKQ
Sbjct: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQVPSQYSSSNPSTPYGAAHPVVSVVEKQ 240

Query: 241 EPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATA 300
           EPECHHT TR SNS SDIKPGPKNFDVK E ASKLD RPPPSDVLEKARAAIASAERATA
Sbjct: 241 EPECHHTLTRISNSVSDIKPGPKNFDVKLEFASKLDTRPPPSDVLEKARAAIASAERATA 300

Query: 301 AARVAAELANVNLGAMKLEGGGQASEC 327
           AARVAAELANVNLGAMKLEGGGQASEC
Sbjct: 301 AARVAAELANVNLGAMKLEGGGQASEC 327

[Close]

BLAST of CmaCh04G020370 vs. NCBI nr
Match: KAG7032495.1 (IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 593.2 bits (1528), Expect = 1.4e-165
Identity = 318/340 (93.53%), Postives = 321/340 (94.41%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLK+MRKEIAQFLQTGQEPIARI
Sbjct: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQTGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP
Sbjct: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRS-------------IIEKLAVSAPPGEL 180
           DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRS             IIEKLAVSAPPGEL
Sbjct: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSVECVSSLSFSSSNIIEKLAVSAPPGEL 180

Query: 181 KLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPY 240
           KLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPY
Sbjct: 181 KLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTPY 240

Query: 241 GES-PVVSVVEKQEPECHHTFTRTSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEK 300
           G + PVVSVVEKQEPECHHTFTR SNSASDIKPGPKN DVKPE ASKLD RPPPSDVLEK
Sbjct: 241 GTAHPVVSVVEKQEPECHHTFTRISNSASDIKPGPKNSDVKPEFASKLDTRPPPSDVLEK 300

Query: 301 ARAAIASAERATAAARVAAELANVNLGAMKLEGGGQASEC 327
           ARAAIASAERATAAARVAAELANVNLGAMK+EGGGQASEC
Sbjct: 301 ARAAIASAERATAAARVAAELANVNLGAMKVEGGGQASEC 340

[Close]

BLAST of CmaCh04G020370 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 358.2 bits (918), Expect = 6.9e-99
Identity = 199/317 (62.78%), Postives = 238/317 (75.08%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MSLLNQLFNRG+F  KCKT L+LAI+R+KLLQNKRD+QLK+M+KEIA FLQ GQEPIARI
Sbjct: 1   MSLLNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEHVIRE N+WAAYEILELFCEF+LARVPI+ES+KECP ELREAIASIIFAAPRCS++P
Sbjct: 61  RVEHVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVP 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           DLLQ+KNLF  KYGKEF+   SELRPDSGVNR+IIEKL+ ++P G  +LK++KEIA+E+ 
Sbjct: 121 DLLQIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYS 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTP-YGESPVVSVVEKQ 240
           L+WDSS+T +EF K+HEDLLGG K +H           S S PS   YG+S V   VE  
Sbjct: 181 LNWDSSATEAEFMKSHEDLLGGAKQIH------RQDGISESRPSQQGYGQSSVSREVESL 240

Query: 241 EPECHHTFTR--TSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERA 300
             E    F +    N  S   P  K   +     +  D R   +DV+E ARAA+ASA+RA
Sbjct: 241 PAEATQRFQKLQAQNPVSKSMPSSK---LTSAFQAPPDTRRNQTDVMEIARAALASADRA 300

Query: 301 TAAARVAAELANVNLGA 315
           TAAAR AA+L NV+ GA
Sbjct: 301 TAAARAAAQLVNVSYGA 308

[Close]

BLAST of CmaCh04G020370 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 263.1 bits (671), Expect = 3.0e-70
Identity = 130/225 (57.78%), Postives = 171/225 (76.00%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MS+L+  FN+G  A KCKT L L I RIKL++N+R+ Q+K MR+EIA+ L+TGQE  ARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEH+IRE+ + AA EILELFCE +  R+PIIE+Q+ECP++L+EAI+S+ FAAPRCSDL 
Sbjct: 61  RVEHIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLT 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHG 180
           +L Q++ LF  KYGKEFV+A SEL+PDSGVNR ++E L+V AP  E KLK++KEIA+EH 
Sbjct: 121 ELQQVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEHE 180

Query: 181 LDWDSSSTASEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPST 226
           LDWD +ST ++  K+HEDLL GPK   G +    P + +     T
Sbjct: 181 LDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKTNLT 225

[Close]

BLAST of CmaCh04G020370 vs. TAIR 10
Match: AT1G25420.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.5e-66
Identity = 143/248 (57.66%), Postives = 175/248 (70.56%), Query Frame = 0


Query: 70  NIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLPDLLQLKNLF 129
           N+WAAYEILELFCEF+LARVPI+ES+KECP ELREAIASIIFAAPRCS++PDLLQ+KNLF
Sbjct: 2   NLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLF 61

Query: 130 AVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDSSSTA 189
             KYGKEF+   SELRPDSGVNR+IIEKL+ ++P G  +LK++KEIA+E+ L+WDSS+T 
Sbjct: 62  GTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATE 121

Query: 190 SEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTP-YGESPVVSVVEKQEPECHHTFT 249
           +EF K+HEDLLGG K +H           S S PS   YG+S V   VE    E    F 
Sbjct: 122 AEFMKSHEDLLGGAKQIH------RQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQ 181

Query: 250 R--TSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATAAARVAAE 309
           +    N  S   P  K   +     +  D R   +DV+E ARAA+ASA+RATAAAR AA+
Sbjct: 182 KLQAQNPVSKSMPSSK---LTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQ 240

Query: 310 LANVNLGA 315
           L NV+ GA
Sbjct: 242 LVNVSYGA 240

[Close]

BLAST of CmaCh04G020370 vs. TAIR 10
Match: AT1G25420.3 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.5e-66
Identity = 143/248 (57.66%), Postives = 175/248 (70.56%), Query Frame = 0


Query: 70  NIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLPDLLQLKNLF 129
           N+WAAYEILELFCEF+LARVPI+ES+KECP ELREAIASIIFAAPRCS++PDLLQ+KNLF
Sbjct: 2   NLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLF 61

Query: 130 AVKYGKEFVSAISELRPDSGVNRSIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDSSSTA 189
             KYGKEF+   SELRPDSGVNR+IIEKL+ ++P G  +LK++KEIA+E+ L+WDSS+T 
Sbjct: 62  GTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATE 121

Query: 190 SEFSKTHEDLLGGPKVVHGAAASQAPSQYSSSNPSTP-YGESPVVSVVEKQEPECHHTFT 249
           +EF K+HEDLLGG K +H           S S PS   YG+S V   VE    E    F 
Sbjct: 122 AEFMKSHEDLLGGAKQIH------RQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQ 181

Query: 250 R--TSNSASDIKPGPKNFDVKPEVASKLDIRPPPSDVLEKARAAIASAERATAAARVAAE 309
           +    N  S   P  K   +     +  D R   +DV+E ARAA+ASA+RATAAAR AA+
Sbjct: 182 KLQAQNPVSKSMPSSK---LTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQ 240

Query: 310 LANVNLGA 315
           L NV+ GA
Sbjct: 242 LVNVSYGA 240

[Close]

BLAST of CmaCh04G020370 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.7e-65
Identity = 130/255 (50.98%), Postives = 171/255 (67.06%), Query Frame = 0


Query: 1   MSLLNQLFNRGVFAGKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQTGQEPIARI 60
           MS+L+  FN+G  A KCKT L L I RIKL++N+R+ Q+K MR+EIA+ L+TGQE  ARI
Sbjct: 1   MSMLDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARI 60

Query: 61  RVEHVIREQNIWAAYEILELFCEFVLARVPIIESQKECPVELREAIASIIFAAPRCSDLP 120
           RVEH+IRE+ + AA EILELFCE +  R+PIIE+Q+ECP++L+EAI+S+ FAAPRCSDL 
Sbjct: 61  RVEHIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLT 120

Query: 121 DLLQLKNLFAVKYGKEFVSAISELRPDSGVNR---------------------------- 180
           +L Q++ LF  KYGKEFV+A SEL+PDSGVNR                            
Sbjct: 121 ELQQVQILFVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNS 180

Query: 181 --SIIEKLAVSAPPGELKLKVMKEIAKEHGLDWDSSSTASEFSKTHEDLLGGPKVVHGAA 226
              ++E L+V AP  E KLK++KEIA+EH LDWD +ST ++  K+HEDLL GPK   G +
Sbjct: 181 ILQLVELLSVRAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDGPKQFGGGS 240

The following BLAST results are available for this feature:

BLAST of CmaCh04G020370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3ZBV1	1.4e-24	37.36	IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1	[more]
P53990	4.2e-24	37.36	IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1	[more]
Q9CX00	4.2e-24	37.36	IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1	[more]
Q568Z6	4.2e-24	37.36	IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1	[more]
Q5R6G8	2.7e-23	36.81	IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1	[more]

BLAST of CmaCh04G020370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I9L3	7.8e-175	100.00	IST1 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470489 PE=3 SV=1	[more]
A0A6J1E6L9	3.5e-167	96.64	IST1 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429884 PE=3 SV=1	[more]
A0A6J1I6R9	2.1e-151	100.00	IST1 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470489 PE=3 SV=1	[more]
A0A6J1DR13	3.8e-145	85.63	IST1 homolog OS=Momordica charantia OX=3673 GN=LOC111022352 PE=3 SV=1	[more]
A0A6J1E1A6	2.4e-144	96.50	IST1 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429884 PE=3 SV=1	[more]

BLAST of CmaCh04G020370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022971809.1	1.6e-174	100.00	IST1 homolog isoform X1 [Cucurbita maxima]	[more]
XP_023538831.1	1.7e-168	97.55	IST1 homolog isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG6601778.1	2.3e-168	97.25	IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022921725.1	7.3e-167	96.64	IST1 homolog isoform X1 [Cucurbita moschata]	[more]
KAG7032495.1	1.4e-165	93.53	IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CmaCh04G020370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G25420.1	6.9e-99	62.78	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G34220.2	3.0e-70	57.78	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G25420.2	3.5e-66	57.66	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G25420.3	3.5e-66	57.66	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G34220.1	1.7e-65	50.98	Regulator of Vps4 activity in the MVB pathway protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005061	Vacuolar protein sorting-associated protein Ist1	PFAM	PF03398	Ist1	coord: 18..181 e-value: 2.1E-62 score: 209.9
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	IST1 FAMILY MEMBER	coord: 14..309
IPR042277	Vacuolar protein sorting-associated protein IST1-like	GENE3D	1.20.1260.60		coord: 5..192 e-value: 5.4E-76 score: 256.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 212..229
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 210..230
None	No IPR available	PANTHER	PTHR12161:SF13	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 14..309

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G020370.1	CmaCh04G020370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport