CmaCh04G020270 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G020270
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionbasic form of pathogenesis-related protein 1-like
LocationCma_Chr04: 12876535 .. 12878873 (+)
RNA-Seq ExpressionCmaCh04G020270
SyntenyCmaCh04G020270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGACTCAAACCCTTCGGATCATGGCCGCTTTCATGGTCACTTTCATTTTCCTCCTCGCCCTAACCAAAGCTCAAAACGCCCCCGGTGACTACCTCGCGCTTCACAACCGAGCTCGAGCCGAGGTCGGCGTCGGGCCCATGCAATGGAGCAACACCGTGGCCGCGTACGCTCAAGCCTATGCGGAAAAAAGAAAGGGTGACTGCGCCATGATTCACTCAACCGGGCCGTACGGGGAAAACATAGCCGCGGGCTACTACCCTGAGTTCACCGGGGCGGATGCAGTGAAGCTGTGGGCGAACGAGAAGCCGCTGTATGATCATGCATCGAATAAATGCGTGGGTGGTGAATGTGGGCACTACACTCAGATGGTGTGGCGGAGCTCGGTGCGGCTTGGATGTGCTAGAGTGCCTTGCAAGGCTAATTCTCAGTTTGTTGTTTGCAATTATGATCCTCCAGGCAACTATATTGGGGAAAAGCCTTATGATTCTTGGGTGGTATGAAGAGATTTGGTTTTAAAATTTTTATGAATATTAATAAAGTTTGTGAGAGAATCATGAATTTATTTATTGAAATAAAACTACGATATTGTCTACAATAGTATTATATTGTCTAGAGCATAAACTCTTGTTGCTGTGTTTTGACTTCCTTACTTATAAACTCATGATCATAGAGTCTCCCCTCTCATTAAGTACACCACATAGAGCCACATTTGAAGCCTATAGATTACCTATGATCATAGAGACTCCCCTCGAACAAAATACACCATAGAGCCTCCCTCGAACAAAATACACCATAGTGCCTCCCCTTAAGTCGATGGATTTCTAAAAGAAACTCCCTTTAATCGAGACTCGACTTCTTTCTTCGGAGACCTTGAACAAAATACGAAATTTGTTCGACACTTTTAACTACGATTTTTAGGCTTACAACTTTTTCGTTCGACATACCTAAGTTAAGTTCATAGTTTGATACCATGTAACTAATCACGAATCTCTATAATGTTATATGATATTTGTCAACTTTGAACATAATATCATGGGTTTGCTTCGAGCTCCCACAAGAAGTCATGTTAATGAAAATGTATTCTTTAGTTATAAATCTATGATCATTTCCGAACAGTCTTCAACACTGAGAGCATCAGCCACCAGCCAATTGTTGTTCCAAGTCACCCGAAAGAAATGCCCTCGGCGGAGATGCGGAAAACCAGTTTAAGGTGTTTTCAGCCTCCAAATCGAGAGAACCACGACGACGGCGATTAACTCCGCTGCAGTATGATACCCACACAGGAGACGAAGACTCACTGCCACAACCTATCGCCGTTTACCAGCTCTGAATATGGAGACCAACGGGGCCGGAGTGACTAAAGGACACCCGCTGCTCAAAAGGCGACCATTTTTTTTCTTACTTTACTTATTGGCACTCCATTCGTCTTTTTCTCGACTTCGTGGGAAAAAAAACAATAATAATAAATAATCAAAATGGTAAAATCAAAGAAATGCCTGTTTTTGTTGAATTACTTAAATTCCCTTTTATCGAAATACTGAAATACTGAAATTTGATAGGTACTGTGATCATTGGGCAATGCTCATGGAAAACTTCTTTAGTACTGGGTTTGGTGGAGAATGTGATTCCTGCACCAATATGACACATTGTTTTAACCGATACCAAGAAGAAGCACATTGATGATCAGAAATTGAAAGATTTCAAAGCAAAGAATTATCTATTTCAAGCATTAGAACGTTCGATCTTGGAGACAACCCTCAACAAGTATACAGAAACTCAATTCATCATACCTGATGATGACGTTGAGAATCCATTGACTTATAAACAGGCAATGAATGATGAAGATAGAGATCTGTGGATCAAAGCCATGAATCTTGAAATGGAGTCAATGTACTTCAATTCAGTCTGGGAACTTGTAGATCAACAAGATGGGGTAAAACCTATTGGTTGCAAATGGATCTATAAGAGGAAACGAGACCAAACCGGTAAGGTACAAACCTTTAAAGCTCGTCTTGTAGAAAAGGGTTATACCCAAAGAGAGGGGGTGGACTATGAAGAAACCTTCTCTCCTGTTGCTATGCTTAAATCGATTAGAATACTCTTGTCTATTGCCACATTTTATGATTATGAAATTTGGCAAATGGATGTCAAGATAGCCTTTCTAAAGGGCAATCTTGAAGAGAGTATCTATATTGCTCAACCAGATGGGTTCATTAAACAAGATCACAAGCATAGGGTTTGCAAGCTTAAAAGATCCATTTATGGGTTGAAGCAAGCATCTCGATCCTGGAATATAAAGTTTGATACCGCGATCAAATCTTATGGCTCGAAATAG

mRNA sequence

ATGTGGACTCAAACCCTTCGGATCATGGCCGCTTTCATGGTCACTTTCATTTTCCTCCTCGCCCTAACCAAAGCTCAAAACGCCCCCGGTGACTACCTCGCGCTTCACAACCGAGCTCGAGCCGAGGTCGGCGTCGGGCCCATGCAATGGAGCAACACCGTGGCCGCGTACGCTCAAGCCTATGCGGAAAAAAGAAAGGGTGACTGCGCCATGATTCACTCAACCGGGCCGTACGGGGAAAACATAGCCGCGGGCTACTACCCTGAGTTCACCGGGGCGGATGCAGTGAAGCTGTGGGCGAACGAGAAGCCGCTGTATGATCATGCATCGAATAAATGCGTGGGTGGTGAATGTGGGCACTACACTCAGATGGTGTGGCGGAGCTCGGTGCGGCTTGGATGTGCTAGAGTGCCTTGCAAGGCTAATTCTCAGTTTGTTGTTTGCAATTATGATCCTCCAGGCAACTATATTGGGGAAAAGCCTTATGATTCTTGGGTGGCAATGAATGATGAAGATAGAGATCTGTGGATCAAAGCCATGAATCTTGAAATGGAGTCAATGTACTTCAATTCAGTCTGGGAACTTGTAGATCAACAAGATGGGGTAAAACCTATTGGTTGCAAATGGATCTATAAGAGGAAACGAGACCAAACCGGTAAGGTACAAACCTTTAAAGCTCGTCTTGTAGAAAAGGGTTATACCCAAAGAGAGGGGGTGGACTATGAAGAAACCTTCTCTCCTGTTGCTATGCTTAAATCGATTAGAATACTCTTGTCTATTGCCACATTTTATGATTATGAAATTTGGCAAATGGATGTCAAGATAGCCTTTCTAAAGGGCAATCTTGAAGAGAGTATCTATATTGCTCAACCAGATGGGTTCATTAAACAAGATCACAAGCATAGGGTTTGCAAGCTTAAAAGATCCATTTATGGGTTGAAGCAAGCATCTCGATCCTGGAATATAAAGTTTGATACCGCGATCAAATCTTATGGCTCGAAATAG

Coding sequence (CDS)

ATGTGGACTCAAACCCTTCGGATCATGGCCGCTTTCATGGTCACTTTCATTTTCCTCCTCGCCCTAACCAAAGCTCAAAACGCCCCCGGTGACTACCTCGCGCTTCACAACCGAGCTCGAGCCGAGGTCGGCGTCGGGCCCATGCAATGGAGCAACACCGTGGCCGCGTACGCTCAAGCCTATGCGGAAAAAAGAAAGGGTGACTGCGCCATGATTCACTCAACCGGGCCGTACGGGGAAAACATAGCCGCGGGCTACTACCCTGAGTTCACCGGGGCGGATGCAGTGAAGCTGTGGGCGAACGAGAAGCCGCTGTATGATCATGCATCGAATAAATGCGTGGGTGGTGAATGTGGGCACTACACTCAGATGGTGTGGCGGAGCTCGGTGCGGCTTGGATGTGCTAGAGTGCCTTGCAAGGCTAATTCTCAGTTTGTTGTTTGCAATTATGATCCTCCAGGCAACTATATTGGGGAAAAGCCTTATGATTCTTGGGTGGCAATGAATGATGAAGATAGAGATCTGTGGATCAAAGCCATGAATCTTGAAATGGAGTCAATGTACTTCAATTCAGTCTGGGAACTTGTAGATCAACAAGATGGGGTAAAACCTATTGGTTGCAAATGGATCTATAAGAGGAAACGAGACCAAACCGGTAAGGTACAAACCTTTAAAGCTCGTCTTGTAGAAAAGGGTTATACCCAAAGAGAGGGGGTGGACTATGAAGAAACCTTCTCTCCTGTTGCTATGCTTAAATCGATTAGAATACTCTTGTCTATTGCCACATTTTATGATTATGAAATTTGGCAAATGGATGTCAAGATAGCCTTTCTAAAGGGCAATCTTGAAGAGAGTATCTATATTGCTCAACCAGATGGGTTCATTAAACAAGATCACAAGCATAGGGTTTGCAAGCTTAAAAGATCCATTTATGGGTTGAAGCAAGCATCTCGATCCTGGAATATAAAGTTTGATACCGCGATCAAATCTTATGGCTCGAAATAG

Protein sequence

MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGHYTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWVAMNDEDRDLWIKAMNLEMESMYFNSVWELVDQQDGVKPIGCKWIYKRKRDQTGKVQTFKARLVEKGYTQREGVDYEETFSPVAMLKSIRILLSIATFYDYEIWQMDVKIAFLKGNLEESIYIAQPDGFIKQDHKHRVCKLKRSIYGLKQASRSWNIKFDTAIKSYGSK
Homology
[Close]
BLAST of CmaCh04G020270 vs. ExPASy Swiss-Prot
Match: P11670 (Basic form of pathogenesis-related protein 1 OS=Nicotiana tabacum OX=4097 PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.8e-52
Identity = 94/157 (59.87%), Postives = 115/157 (73.25%), Query Frame = 0 

Query: 6   LRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKR 65
           L  + A  +TF  L+  +KAQN+P DYL  HN AR +VGVGPM W N +AAYAQ YA +R
Sbjct: 4   LTTIVACFITFAILIHSSKAQNSPQDYLNPHNAARRQVGVGPMTWDNRLAAYAQNYANQR 63

Query: 66  KGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGHYTQMV 125
            GDC MIHS GPYGEN+AA  +P+   A AVK+W +EK  YD+ SN CVGG CGHYTQ+V
Sbjct: 64  IGDCGMIHSHGPYGENLAAA-FPQLNAAGAVKMWVDEKRFYDYNSNSCVGGVCGHYTQVV 123

Query: 126 WRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPY 163
           WR+SVRLGCARV       F+ CNYDPPGN+IG++P+
Sbjct: 124 WRNSVRLGCARVRSNNGWFFITCNYDPPGNFIGQRPF 159

[Close]
BLAST of CmaCh04G020270 vs. ExPASy Swiss-Prot
Match: P33154 (Pathogenesis-related protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g14610 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.2e-45
Identity = 84/155 (54.19%), Postives = 111/155 (71.61%), Query Frame = 0 

Query: 12  FMVTFIFLLAL----TKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKRKG 71
           F++ F+ L+      +KAQ++P DYL +HN+AR  VGVGPMQW   VAAYA++YAE+ +G
Sbjct: 9   FLIVFVALVGALVLPSKAQDSPQDYLRVHNQARGAVGVGPMQWDERVAAYARSYAEQLRG 68

Query: 72  DCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGHYTQMVWR 131
           +C +IHS GPYGEN+A G   + +G  AV +W +EK  Y++A+N C  G CGHYTQ+VWR
Sbjct: 69  NCRLIHSGGPYGENLAWG-SGDLSGVSAVNMWVSEKANYNYAANTC-NGVCGHYTQVVWR 128

Query: 132 SSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPY 163
            SVRLGCA+V C      + CNYDP GNY+ EKPY
Sbjct: 129 KSVRLGCAKVRCNNGGTIISCNYDPRGNYVNEKPY 161

[Close]
BLAST of CmaCh04G020270 vs. ExPASy Swiss-Prot
Match: P08299 (Pathogenesis-related protein 1A OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.7e-44
Identity = 82/156 (52.56%), Postives = 105/156 (67.31%), Query Frame = 0 

Query: 8   IMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKRKG 67
           ++ + ++ F+ +    +AQN+  DYL  HN ARA+VGV P+ W + VAAYAQ YA +   
Sbjct: 13  LLVSTLLLFLVISHSCRAQNSQQDYLDAHNTARADVGVEPLTWDDQVAAYAQNYASQLAA 72

Query: 68  DCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGE-CGHYTQMVW 127
           DC ++HS G YGEN+A G     T A AV++W +EK  YDH SN C  G+ CGHYTQ+VW
Sbjct: 73  DCNLVHSHGQYGENLAEGSGDFMTAAKAVEMWVDEKQYYDHDSNTCAQGQVCGHYTQVVW 132

Query: 128 RSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPY 163
           R+SVR+GCARV C      V CNYDPPGNY GE PY
Sbjct: 133 RNSVRVGCARVQCNNGGYVVSCNYDPPGNYRGESPY 168

[Close]
BLAST of CmaCh04G020270 vs. ExPASy Swiss-Prot
Match: Q04108 (Pathogenesis-related leaf protein 4 OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.1e-43
Identity = 85/156 (54.49%), Postives = 114/156 (73.08%), Query Frame = 0 

Query: 11  AFMVTFIFLLAL---TKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKRKG 70
           + ++T + +LA+    +AQN+P DYLA+HN ARA+VGVGPM W   +A+ AQ YA  R G
Sbjct: 7   SLLLTCLMVLAIFHSCEAQNSPQDYLAVHNDARAQVGVGPMSWDANLASRAQNYANSRAG 66

Query: 71  DCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGE-CGHYTQMVW 130
           DC +IHS    GEN+A G   +FTG  AV+LW +E+P Y++A+N+CVGG+ CGHYTQ+VW
Sbjct: 67  DCNLIHSGA--GENLAKG-GGDFTGRAAVQLWVSERPDYNYATNQCVGGKMCGHYTQVVW 126

Query: 131 RSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPY 163
           R+SVRLGC R  C     F+ CNYDP GN++GE+PY
Sbjct: 127 RNSVRLGCGRARCNNGWWFISCNYDPVGNWVGERPY 159

[Close]
BLAST of CmaCh04G020270 vs. ExPASy Swiss-Prot
Match: P07053 (Pathogenesis-related protein 1B OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.8e-43
Identity = 81/155 (52.26%), Postives = 103/155 (66.45%), Query Frame = 0 

Query: 9   MAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKRKGD 68
           + + ++ F+ +   + AQN+  DYL  HN ARA+VGV P+ W N VAAYAQ Y  +   D
Sbjct: 14  LVSTLLLFLIISHSSHAQNSQQDYLDAHNTARADVGVEPLTWDNGVAAYAQNYVSQLAAD 73

Query: 69  CAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGE-CGHYTQMVWR 128
           C ++HS G YGEN+A G     T A AV++W +EK  YDH SN C  G+ CGHYTQ+VWR
Sbjct: 74  CNLVHSHGQYGENLAQGSGDFMTAAKAVEMWVDEKQYYDHDSNTCAQGQVCGHYTQVVWR 133

Query: 129 SSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPY 163
           +SVR+GCARV C      V CNYDPPGN IG+ PY
Sbjct: 134 NSVRVGCARVKCNNGGYVVSCNYDPPGNVIGQSPY 168

[Close]
BLAST of CmaCh04G020270 vs. ExPASy TrEMBL
Match: A0A6J1I475 (basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111470480 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 6.3e-95
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 166

[Close]
BLAST of CmaCh04G020270 vs. ExPASy TrEMBL
Match: A0A6J1ICC8 (basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111474223 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 6.3e-95
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 28  MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 87

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 88  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 147

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 148 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 193

[Close]
BLAST of CmaCh04G020270 vs. ExPASy TrEMBL
Match: A0A6J1IEK2 (basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111473362 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 6.3e-95
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 166

[Close]
BLAST of CmaCh04G020270 vs. ExPASy TrEMBL
Match: A0A6J1IC05 (basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111473363 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.8e-94
Identity = 165/166 (99.40%), Postives = 165/166 (99.40%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 28  MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 87

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIA GYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 88  YAEKRKGDCAMIHSTGPYGENIAVGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 147

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 148 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 193

[Close]
BLAST of CmaCh04G020270 vs. ExPASy TrEMBL
Match: A0A6J1IIB6 (basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111473367 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.8e-94
Identity = 165/166 (99.40%), Postives = 166/166 (100.00%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 28  MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 87

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 88  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 147

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YT+MVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 148 YTKMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 193

[Close]
BLAST of CmaCh04G020270 vs. NCBI nr
Match: XP_022971803.1 (basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022971804.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022971806.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022974680.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022974686.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022974689.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022974690.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022975182.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022975183.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima])

HSP 1 Score: 357.5 bits (916), Expect = 1.3e-94
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 28  MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 87

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 88  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 147

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 148 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 193

[Close]
BLAST of CmaCh04G020270 vs. NCBI nr
Match: XP_022971801.1 (basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022971802.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022974677.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022974684.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022974688.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022975326.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_022975679.1 basic form of pathogenesis-related protein 1-like [Cucurbita maxima])

HSP 1 Score: 357.5 bits (916), Expect = 1.3e-94
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 166

[Close]
BLAST of CmaCh04G020270 vs. NCBI nr
Match: XP_022974675.1 (basic form of pathogenesis-related protein 1-like, partial [Cucurbita maxima])

HSP 1 Score: 357.5 bits (916), Expect = 1.3e-94
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 166

[Close]
BLAST of CmaCh04G020270 vs. NCBI nr
Match: XP_022974676.1 (basic form of pathogenesis-related protein 1-like [Cucurbita maxima])

HSP 1 Score: 355.9 bits (912), Expect = 3.8e-94
Identity = 165/166 (99.40%), Postives = 165/166 (99.40%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 28  MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 87

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIA GYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 88  YAEKRKGDCAMIHSTGPYGENIAVGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 147

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 148 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 193

[Close]
BLAST of CmaCh04G020270 vs. NCBI nr
Match: XP_022974679.1 (basic form of pathogenesis-related protein 1-like [Cucurbita maxima])

HSP 1 Score: 355.9 bits (912), Expect = 3.8e-94
Identity = 165/166 (99.40%), Postives = 166/166 (100.00%), Query Frame = 0 

Query: 1   MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 60
           MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA
Sbjct: 28  MWTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQA 87

Query: 61  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 120
           YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH
Sbjct: 88  YAEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGH 147

Query: 121 YTQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 167
           YT+MVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV
Sbjct: 148 YTKMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPYDSWV 193

[Close]
BLAST of CmaCh04G020270 vs. TAIR 10
Match: AT4G33720.1 (CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein )

HSP 1 Score: 186.8 bits (473), Expect = 2.8e-47
Identity = 86/148 (58.11%), Postives = 108/148 (72.97%), Query Frame = 0 

Query: 16  FIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKRKGDCAMIHST 75
           F+ L+   KAQ++P D+LA+HNRARAEVGVGP++W   VAAYA+ YA +RKGDCAM HS+
Sbjct: 17  FLVLIVHLKAQDSPQDFLAVHNRARAEVGVGPLRWDEKVAAYARNYANQRKGDCAMKHSS 76

Query: 76  GPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCV-GGECGHYTQMVWRSSVRLGC 135
           G YGENIA       TG  AV +W +E+  YD+ SN C    +CGHYTQ+VWR+S RLGC
Sbjct: 77  GSYGENIAWS-SGSMTGVAAVDMWVDEQFDYDYDSNTCAWDKQCGHYTQVVWRNSERLGC 136

Query: 136 ARVPCKANSQFVVCNYDPPGNYIGEKPY 163
           A+V C     F+ CNYDPPGN++GE PY
Sbjct: 137 AKVRCNNGQTFITCNYDPPGNWVGEWPY 163

[Close]
BLAST of CmaCh04G020270 vs. TAIR 10
Match: AT2G14610.1 (pathogenesis-related gene 1 )

HSP 1 Score: 185.3 bits (469), Expect = 8.2e-47
Identity = 84/155 (54.19%), Postives = 111/155 (71.61%), Query Frame = 0 

Query: 12  FMVTFIFLLAL----TKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKRKG 71
           F++ F+ L+      +KAQ++P DYL +HN+AR  VGVGPMQW   VAAYA++YAE+ +G
Sbjct: 9   FLIVFVALVGALVLPSKAQDSPQDYLRVHNQARGAVGVGPMQWDERVAAYARSYAEQLRG 68

Query: 72  DCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGHYTQMVWR 131
           +C +IHS GPYGEN+A G   + +G  AV +W +EK  Y++A+N C  G CGHYTQ+VWR
Sbjct: 69  NCRLIHSGGPYGENLAWG-SGDLSGVSAVNMWVSEKANYNYAANTC-NGVCGHYTQVVWR 128

Query: 132 SSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPY 163
            SVRLGCA+V C      + CNYDP GNY+ EKPY
Sbjct: 129 KSVRLGCAKVRCNNGGTIISCNYDPRGNYVNEKPY 161

[Close]
BLAST of CmaCh04G020270 vs. TAIR 10
Match: AT1G50060.1 (CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 6.9e-46
Identity = 83/151 (54.97%), Postives = 102/151 (67.55%), Query Frame = 0 

Query: 13  MVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKRKGDCAMI 72
           +V   FL+  T AQN P DYL  HN ARA+VGV  + W  T+AAYA  Y+  RK DC ++
Sbjct: 11  IVAISFLVVATNAQNTPQDYLNSHNTARAQVGVPNVVWDTTLAAYALNYSNFRKADCNLV 70

Query: 73  HSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGG-ECGHYTQMVWRSSVR 132
           HS GPYGEN+A G    F+   AVKLW +EKP Y +A N C GG +C HYTQ+VWR SV+
Sbjct: 71  HSNGPYGENLAKGSSSSFSAISAVKLWVDEKPYYSYAYNNCTGGKQCLHYTQVVWRDSVK 130

Query: 133 LGCARVPCKANSQFVVCNYDPPGNYIGEKPY 163
           +GCARV C     FV CNY+ PGN++GE PY
Sbjct: 131 IGCARVQCTNTWWFVSCNYNSPGNWVGEYPY 161

[Close]
BLAST of CmaCh04G020270 vs. TAIR 10
Match: AT2G14580.1 (basic pathogenesis-related protein 1 )

HSP 1 Score: 176.4 bits (446), Expect = 3.8e-44
Identity = 82/161 (50.93%), Postives = 111/161 (68.94%), Query Frame = 0 

Query: 2   WTQTLRIMAAFMVTFIFLLALTKAQNAPGDYLALHNRARAEVGVGPMQWSNTVAAYAQAY 61
           +++ L I+AA +   +  L   KAQ++  DY+  HN+AR+++GVGPMQW   +AAYA+ Y
Sbjct: 6   YSRILIILAALVGALVVPL---KAQDSQQDYVNAHNQARSQIGVGPMQWDEGLAAYARNY 65

Query: 62  AEKRKGDCAMIHSTGPYGENIAAGYYPEFTGADAVKLWANEKPLYDHASNKCVGGECGHY 121
           A + KGDC ++HS GPYGEN+A     + +G  AV LW NEK  Y++ +N C  G CGHY
Sbjct: 66  ANQLKGDCRLVHSRGPYGENLAKS-GGDLSGVAAVNLWVNEKANYNYDTNTC-NGVCGHY 125

Query: 122 TQMVWRSSVRLGCARVPCKANSQFVVCNYDPPGNYIGEKPY 163
           TQ+VWR+SVRLGCA+V C      + CNYDPPGNY  +KPY
Sbjct: 126 TQVVWRNSVRLGCAKVRCNNGGTIISCNYDPPGNYANQKPY 161

[Close]
BLAST of CmaCh04G020270 vs. TAIR 10
Match: AT2G19990.1 (pathogenesis-related protein-1-like )

HSP 1 Score: 173.3 bits (438), Expect = 3.2e-43
Identity = 80/136 (58.82%), Postives = 100/136 (73.53%), Query Frame = 0 

Query: 29  PGDYLALHNRARAEVGVGPMQWSNTVAAYAQAYAEKRKGDCAMIHSTGPYGENIAAGYYP 88
           P + L +HN+ARA VGVGPM W+ T+A YAQ+YA +R  DCAM HS GP+GEN+AAG + 
Sbjct: 42  PQETLVVHNKARAMVGVGPMVWNETLATYAQSYAHERARDCAMKHSLGPFGENLAAG-WG 101

Query: 89  EFTGADAVKLWANEKPLYDHASNKCVG-GECGHYTQMVWRSSVRLGCARVPCKANSQ-FV 148
             +G  A + W  EK  YD+ SN C G G CGHYTQ+VWR SVRLGCA V CK +   +V
Sbjct: 102 TMSGPVATEYWMTEKENYDYDSNTCGGDGVCGHYTQIVWRDSVRLGCASVRCKNDEYIWV 161

Query: 149 VCNYDPPGNYIGEKPY 163
           +C+YDPPGNYIG++PY
Sbjct: 162 ICSYDPPGNYIGQRPY 176

The following BLAST results are available for this feature:
BLAST of CmaCh04G020270 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P116702.8e-5259.87Basic form of pathogenesis-related protein 1 OS=Nicotiana tabacum OX=4097 PE=3 S... [more]
P331541.2e-4554.19Pathogenesis-related protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g14610 PE=1... [more]
P082993.7e-4452.56Pathogenesis-related protein 1A OS=Nicotiana tabacum OX=4097 PE=1 SV=1[more]
Q041081.1e-4354.49Pathogenesis-related leaf protein 4 OS=Solanum lycopersicum OX=4081 PE=2 SV=1[more]
P070531.8e-4352.26Pathogenesis-related protein 1B OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
BLAST of CmaCh04G020270 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1I4756.3e-95100.00basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1ICC86.3e-95100.00basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1IEK26.3e-95100.00basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1IC051.8e-9499.40basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1IIB61.8e-9499.40basic form of pathogenesis-related protein 1-like OS=Cucurbita maxima OX=3661 GN... [more]
BLAST of CmaCh04G020270 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022971803.11.3e-94100.00basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_0229718... [more]
XP_022971801.11.3e-94100.00basic form of pathogenesis-related protein 1-like [Cucurbita maxima] >XP_0229718... [more]
XP_022974675.11.3e-94100.00basic form of pathogenesis-related protein 1-like, partial [Cucurbita maxima][more]
XP_022974676.13.8e-9499.40basic form of pathogenesis-related protein 1-like [Cucurbita maxima][more]
XP_022974679.13.8e-9499.40basic form of pathogenesis-related protein 1-like [Cucurbita maxima][more]
BLAST of CmaCh04G020270 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G33720.12.8e-4758.11CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 pro... [more]
AT2G14610.18.2e-4754.19pathogenesis-related gene 1 [more]
AT1G50060.16.9e-4654.97CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 pro... [more]
AT2G14580.13.8e-4450.93basic pathogenesis-related protein 1 [more]
AT2G19990.13.2e-4358.82pathogenesis-related protein-1-like [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002413Venom allergen 5-likePRINTSPR00838V5ALLERGENcoord: 93..108
score: 30.47
coord: 117..136
score: 49.69
coord: 48..66
score: 27.63
IPR001283Cysteine-rich secretory protein-relatedPRINTSPR00837V5TPXLIKEcoord: 95..108
score: 38.5
coord: 118..134
score: 63.28
coord: 48..66
score: 36.79
coord: 145..158
score: 58.05
IPR001283Cysteine-rich secretory protein-relatedPANTHERPTHR10334CYSTEINE-RICH SECRETORY PROTEIN-RELATEDcoord: 19..162
IPR014044CAP domainSMARTSM00198SCP_3coord: 27..158
e-value: 4.0E-59
score: 212.4
IPR014044CAP domainPFAMPF00188CAPcoord: 33..150
e-value: 1.7E-22
score: 80.6
IPR035940CAP superfamilyGENE3D3.40.33.10CAPcoord: 23..169
e-value: 4.6E-44
score: 152.2
IPR035940CAP superfamilySUPERFAMILY55797PR-1-likecoord: 24..164
IPR013103Reverse transcriptase, RNA-dependent DNA polymerasePFAMPF07727RVT_2coord: 190..331
e-value: 1.2E-46
score: 159.3
NoneNo IPR availablePANTHERPTHR10334:SF488PATHOGENESIS-RELATED PROTEIN 1coord: 19..162
NoneNo IPR availableCDDcd05381CAP_PR-1coord: 29..162
e-value: 4.63584E-86
score: 253.325
IPR018244Allergen V5/Tpx-1-related, conserved sitePROSITEPS01010CRISP_2coord: 145..156
IPR018244Allergen V5/Tpx-1-related, conserved sitePROSITEPS01009CRISP_1coord: 119..129
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 190..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G020270.1CmaCh04G020270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009607 response to biotic stimulus
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane