CmaCh04G019580 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G019580
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDNA-directed RNA polymerase subunit beta
LocationCma_Chr04: 10968829 .. 10971110 (-)
RNA-Seq ExpressionCmaCh04G019580
SyntenyCmaCh04G019580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCCAGAAATCAGGGAAGGGGGTTCGAGTGAGAATCCAGATATGATTCGTGTATCTATTTCACAGAAATGGGAAGGAAATCAAAGTAGGCGATAAGGTAGCGGGAAGACATGGAAATCAAGGTATTCTTTCACAAATGGTGCCTAGAGAAGATATGTCTTATTTGCAGAAGACCAGTTGATATGGTTTGAAATCCATTAGGAGTCCCCTCACGAATGAATGTAGGACAAATATTTGTTTGAGTACTCACTCGGCCGGGTGGTTAGCGGGGAGTTTTCTAGATAGACATTATCTCATAGTACCTTTTGTTTGATGAGAGATATGAACAAGTGGCTTCTAGAAAACAACTAGTCTTTTCTTTCCTCATTCTATGAAGCCAGTAAGCAAACAGCGAGTCCATAGGTATTTGAACCCTAGTATCCGGGAAAAAGCAGATTGATGGAAGAACGGGAAATCCTTTTGAACAACCTGTTATAATAGGAAAGCCTTATATCTTGAAATGAATGAATCAAGTTGATGTTAATACACAGACGTTCGTTCCTGTGGACATTATGTACTTGTTACACAACAACCCCTTATAGGAAGGGCCAAGCAAGGCAGACAACGGGTGGAAGAAATGGAGGTTTGGGCTCTAGAAGGATTTGGTGTTGATTTTATTTGACAAGAGATGCTTACTTATCAATCTAATCATATTAGAGCTCGGCAAGAAGTACTTGGTACTACGATCATTGGAGGAAGAATACCTAAACCTGAGGATACTCCAAAATCTTTTCAATTCCTCGTTCGAGAATTACGATCTTTGGCTTTGGAACGGAATAATTTCCTTGTATCTGAGAATAACTTCTAGATTAAGAGGAAGGAAGCTTAATCAAAATGAATCAGACAATTTGTTCTATGATCGATAGGTATAAACATAACTCCGAATGAATTGGATTAGTTTCTCCTCAACAAATAAGTTTAGTGCTTGGGCCAATAAAACCCTAGCTAATGTCGAGATAGTTGGAGAGGTAACAAAACCCTATACTTCTTTTCATTACAAAACGAATAAACCTGAAATGGATTATTTTGTGAAAGACTTTTCGGGCCTATAAAAAGGGGGATTTGTGCTTGTGGAATTTCTTGAGTAATCGGAGAAAGAAGATTCGCGGTGAACAATGCGTAGTCTAATTTGTTGGTTGATTCTCAGATACGAAGAAATGGGCTTTATTAAACAAGCTGGCATGCCCAGTAACCCATGTGTGGTGTTTGAAACGTCTTCCTAGTTATATCGGGAATCCTTTAGCTTTAGATAAACCTCTTAAAGAATTAGAAGGCTTAGTATATTGCGATGTGTGATTTGATGTTGATTTTACGTATTCCCAATGAGAAACAGTCATCCCATTCATTCAATCTCATTGGGATGCCCCGGATCTGATATGTGGCTTGCTTGGTAGGATTACCATGAAGCTTAGAATTGTGAGTGTAGTCAATACTCCCATCCCAAATCAAAGGGGAATTAGTCTATGGTCGATTCAGTAATAAATAGGAATTATCAGTTCTACCTCGTGAAAGAAAACAGATTTCTTTTGTGTAATTAACCGTTTCTTTTTCTTTCATAAATTATGCTACAATAAGCAATTCATGGTGAAAGGAGTCTATCCATCGCATATAGGCTTTAAGGACATCGTGGCATAACTGTCGAGGTGAATGAAGTAGGGCCCTAACTAAAAGATCGAATGGAACGGTACAAAGACAAGTAAATCAATCCCTTATTCCAAGGGACTCCTTATTAATTTGAATTAAGGGGATTCATCATTCCAAGGGAAATAAACTACTCAGAAATTGCACATTTCTTTTGTTTCCATTGTAATTGATATAAAGAACTCAGTCAACATTTAAATCACAAAGTCAAAGAATCCATGAAATGTTGTTTGGTCTTAATTGAGAACTTGAGTAAAGAGTAGTTGGGGGTTTTATAGAAGTTGTTGGATAGTGGGAACTCCTTTATTTGATGTAACTACTTGAGCTGAGTGGGATGAAAAGAAACTTTCACGTCCGATTTGGAAAGGGGGAGATCCTATAGTATCCTATATTTGTCTTTTGCTAGGCCCATAGCTAAAAACCCTACTTTCGTACGATCACGGGGTTCATTCGAATATGAAATCCAATCCGGGAAATACAGCATCCCACTTTTTGTGACTACCCAAGGCTTGCTTCGATACATTTCGAAATCAAGAAATTTCTACCGGAGCAGGTGCTATACGAGAACAATTAGTCGATCCGAATTTGCGACTTATTAG

mRNA sequence

ATGGATCCAGAAATCAGGGAAGGGGGTTCGAGTGAGAATCCAGATATGATTCGTGAAATCAAAGTAGGCGATAAGGTAGCGGGAAGACATGGAAATCAAGGTATTCTTTCACAAATGGTGCCTAGAGAAGATATGTCTTATTTGCAGAAGACCAACGTTCGTTCCTGTGGACATTATGTACTTGTTACACAACAACCCCTTATAGGAAGGGCCAAGCAAGGCAGACAACGGGTGGAAGAAATGGAGAGATGCTTACTTATCAATCTAATCATATTAGAGCTCGGCAAGAAGTACTTGGTACTACGATCATTGGAGGAAGAATACCTAAACCTGAGGATACTCCAAAATCTTTTCAATTCCTCGTTCGAGAATTACGATCTTTGGCTTTGGAACGGAATAATTTCCTTGTATCTGAGAATAACTTCTAGATTAAGAGGAAGGAAGCTTAATCAAAATGAATCAGACAATTTGCCCATAGCTAAAAACCCTACTTTCGTACGATCACGGGGTTCATTCGAATATGAAATCCAATCCGGGAAATACAGCATCCCACTTTTTGTGACTACCCAAGGCTTGCTTCGATACATTTCGAAATCAAGAAATTTCTACCGGAGCAGGTGCTATACGAGAACAATTAGTCGATCCGAATTTGCGACTTATTAG

Coding sequence (CDS)

ATGGATCCAGAAATCAGGGAAGGGGGTTCGAGTGAGAATCCAGATATGATTCGTGAAATCAAAGTAGGCGATAAGGTAGCGGGAAGACATGGAAATCAAGGTATTCTTTCACAAATGGTGCCTAGAGAAGATATGTCTTATTTGCAGAAGACCAACGTTCGTTCCTGTGGACATTATGTACTTGTTACACAACAACCCCTTATAGGAAGGGCCAAGCAAGGCAGACAACGGGTGGAAGAAATGGAGAGATGCTTACTTATCAATCTAATCATATTAGAGCTCGGCAAGAAGTACTTGGTACTACGATCATTGGAGGAAGAATACCTAAACCTGAGGATACTCCAAAATCTTTTCAATTCCTCGTTCGAGAATTACGATCTTTGGCTTTGGAACGGAATAATTTCCTTGTATCTGAGAATAACTTCTAGATTAAGAGGAAGGAAGCTTAATCAAAATGAATCAGACAATTTGCCCATAGCTAAAAACCCTACTTTCGTACGATCACGGGGTTCATTCGAATATGAAATCCAATCCGGGAAATACAGCATCCCACTTTTTGTGACTACCCAAGGCTTGCTTCGATACATTTCGAAATCAAGAAATTTCTACCGGAGCAGGTGCTATACGAGAACAATTAGTCGATCCGAATTTGCGACTTATTAG

Protein sequence

MDPEIREGGSSENPDMIREIKVGDKVAGRHGNQGILSQMVPREDMSYLQKTNVRSCGHYVLVTQQPLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRSLEEEYLNLRILQNLFNSSFENYDLWLWNGIISLYLRITSRLRGRKLNQNESDNLPIAKNPTFVRSRGSFEYEIQSGKYSIPLFVTTQGLLRYISKSRNFYRSRCYTRTISRSEFATY
Homology
BLAST of CmaCh04G019580 vs. ExPASy Swiss-Prot
Match: A7Y3B9 (DNA-directed RNA polymerase subunit beta OS=Ipomoea purpurea OX=4121 GN=rpoB PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.0e-10
Identity = 33/51 (64.71%), Postives = 42/51 (82.35%), Query Frame = 0

Query: 6   REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ 50
           ++GGSS NP+MI       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ
Sbjct: 790 KKGGSSYNPEMIRVYILQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQ 840


HSP 2 Score: 49.7 bits (117), Expect = 5.0e-05
Identity = 28/54 (51.85%), Postives = 34/54 (62.96%), Query Frame = 0

Query: 50   KTNVRSCGHYVLVTQQPLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS 104
            K + RS GHY LVTQQPL GRAKQG QRV EME   L    +  + ++ L  +S
Sbjct: 954  KIHGRSSGHYALVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKS 1007

BLAST of CmaCh04G019580 vs. ExPASy Swiss-Prot
Match: Q0G9M7 (DNA-directed RNA polymerase subunit beta OS=Liriodendron tulipifera OX=3415 GN=rpoB PE=3 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 1.0e-10
Identity = 33/51 (64.71%), Postives = 42/51 (82.35%), Query Frame = 0

Query: 6   REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ 50
           ++GGSS NP+MI       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ
Sbjct: 793 KKGGSSYNPEMIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQ 843


HSP 2 Score: 51.2 bits (121), Expect = 1.7e-05
Identity = 28/54 (51.85%), Postives = 34/54 (62.96%), Query Frame = 0

Query: 50   KTNVRSCGHYVLVTQQPLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS 104
            K + RS GHY LVTQQPL GRAKQG QRV EME   L    +  + ++ L  +S
Sbjct: 957  KIHGRSSGHYALVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHISQEMLTYKS 1010

BLAST of CmaCh04G019580 vs. ExPASy Swiss-Prot
Match: Q68S14 (DNA-directed RNA polymerase subunit beta OS=Panax ginseng OX=4054 GN=rpoB PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 33/51 (64.71%), Postives = 41/51 (80.39%), Query Frame = 0

Query: 6   REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ 50
           + GGSS NP+MI       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ
Sbjct: 794 KRGGSSYNPEMIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQ 844


HSP 2 Score: 49.7 bits (117), Expect = 5.0e-05
Identity = 28/54 (51.85%), Postives = 34/54 (62.96%), Query Frame = 0

Query: 50   KTNVRSCGHYVLVTQQPLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS 104
            K + RS GHY LVTQQPL GRAKQG QRV EME   L    +  + ++ L  +S
Sbjct: 958  KIHGRSSGHYALVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKS 1011

BLAST of CmaCh04G019580 vs. ExPASy Swiss-Prot
Match: Q06H05 (DNA-directed RNA polymerase subunit beta OS=Drimys granadensis OX=224735 GN=rpoB PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.3e-10
Identity = 33/49 (67.35%), Postives = 40/49 (81.63%), Query Frame = 0

Query: 8   GGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ 50
           GGSS NP+MI       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ
Sbjct: 796 GGSSYNPEMIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQ 844


HSP 2 Score: 51.2 bits (121), Expect = 1.7e-05
Identity = 28/54 (51.85%), Postives = 34/54 (62.96%), Query Frame = 0

Query: 50   KTNVRSCGHYVLVTQQPLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS 104
            K + RS GHY LVTQQPL GRAKQG QRV EME   L    +  + ++ L  +S
Sbjct: 958  KIHGRSSGHYALVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHISQEMLTYKS 1011

BLAST of CmaCh04G019580 vs. ExPASy Swiss-Prot
Match: P50546 (DNA-directed RNA polymerase subunit beta OS=Arabidopsis thaliana OX=3702 GN=rpoB PE=3 SV=4)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-10
Identity = 32/51 (62.75%), Postives = 42/51 (82.35%), Query Frame = 0

Query: 6   REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ 50
           ++GGSS NP++I       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ
Sbjct: 795 KKGGSSYNPEIIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQ 845


HSP 2 Score: 48.5 bits (114), Expect = 1.1e-04
Identity = 27/54 (50.00%), Postives = 34/54 (62.96%), Query Frame = 0

Query: 50   KTNVRSCGHYVLVTQQPLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS 104
            K + RS GHY LVTQQPL GR+KQG QRV EME   L    +  + ++ L  +S
Sbjct: 959  KIHGRSSGHYALVTQQPLRGRSKQGGQRVGEMEVWALEGFGVAHILQEMLTYKS 1012

BLAST of CmaCh04G019580 vs. ExPASy TrEMBL
Match: A0A3N7FZF8 (DNA-directed RNA polymerase OS=Populus trichocarpa OX=3694 GN=POPTR_013G142232 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 3.3e-28
Identity = 98/260 (37.69%), Postives = 113/260 (43.46%), Query Frame = 0

Query: 6   REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ--------- 65
           ++GGSS NP+ I       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ         
Sbjct: 284 KKGGSSYNPETIRVYILQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGAPVDMVF 343

Query: 66  ------------------------------------------------------------ 125
                                                                       
Sbjct: 344 NPLGVPSRMNVGQIFECSLGLAGSLLDRHYRVAPFDERYEQEASRKLVFSELYEAGKQTG 403

Query: 126 --------------------------------------------KTNVRSCGHYVLVTQQ 132
                                                       K + RS GHY LVTQQ
Sbjct: 404 NPWVFEPECPGKSRIFDGRTGDPFEQPVIIGKPYILKLIHQVADKIHGRSSGHYALVTQQ 463

BLAST of CmaCh04G019580 vs. ExPASy TrEMBL
Match: A0A803N320 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.9e-23
Identity = 86/236 (36.44%), Postives = 99/236 (41.95%), Query Frame = 0

Query: 18  REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ---------------------------- 77
           REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ                            
Sbjct: 303 REIKVGDKVAGRHGNKGIISRILPRQDMPYLQDRRPVDMIFNPLGVPSRMNVGQIFEYSL 362

Query: 78  ------------------------------------------------------------ 132
                                                                       
Sbjct: 363 GLAGSLLDRHYRIPPFDERYEQEPSRKLVCSELYEASKQTANPWVFEPEYPGKSQIFDGR 422

BLAST of CmaCh04G019580 vs. ExPASy TrEMBL
Match: A0A482AWY8 (DNA-directed RNA polymerase subunit beta OS=Aconitum brachypodum OX=226103 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.5e-20
Identity = 100/318 (31.45%), Postives = 130/318 (40.88%), Query Frame = 0

Query: 6    REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ--------- 65
            ++GGSS NP+ I       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ         
Sbjct: 793  KKGGSSYNPETIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGTPVDMVF 852

Query: 66   ------------------------------------------------------------ 125
                                                                        
Sbjct: 853  NPLGVPSRMNVGQIFECSLGLAGDLLDRHYRISPFDERYEQEASRKLVFSELYEASKQTA 912

Query: 126  --------------------------------------------KTNVRSCGHYVLVTQQ 185
                                                        K + RS GHY LVTQQ
Sbjct: 913  NPWVFEPEYPGKSRIFDGRTGDPFEQPVIIGKSYILKLIHQVDDKIHGRSSGHYALVTQQ 972

Query: 186  PLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS----LEEEYLNLRILQNLFNS- 192
            PL GRAKQG QRV EME   L    +  + ++ L  +S      +E L   I+    ++ 
Sbjct: 973  PLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHMRARQEVLGTTIIGGTISNP 1032

BLAST of CmaCh04G019580 vs. ExPASy TrEMBL
Match: A0A7T1WQB9 (RNA polymerase beta' subunit OS=Aconitum flavum OX=286045 GN=rpoC1 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.5e-20
Identity = 100/318 (31.45%), Postives = 130/318 (40.88%), Query Frame = 0

Query: 6    REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ--------- 65
            ++GGSS NP+ I       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ         
Sbjct: 793  KKGGSSYNPETIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGTPVDMVF 852

Query: 66   ------------------------------------------------------------ 125
                                                                        
Sbjct: 853  NPLGVPSRMNVGQIFECSLGLAGDLLDRHYRISPFDERYEQEASRKLVFSELYEASKQTA 912

Query: 126  --------------------------------------------KTNVRSCGHYVLVTQQ 185
                                                        K + RS GHY LVTQQ
Sbjct: 913  NPWVFEPEYPGKSRIFDGRTGDPFEQPVIIGKSYILKLIHQVDDKIHGRSSGHYALVTQQ 972

Query: 186  PLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS----LEEEYLNLRILQNLFNS- 192
            PL GRAKQG QRV EME   L    +  + ++ L  +S      +E L   I+    ++ 
Sbjct: 973  PLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHMRARQEVLGTTIIGGTISNP 1032

BLAST of CmaCh04G019580 vs. ExPASy TrEMBL
Match: A0A7J6EJ59 (Multifunctional fusion protein OS=Cannabis sativa OX=3483 GN=G4B88_017242 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 4.1e-18
Identity = 102/327 (31.19%), Postives = 127/327 (38.84%), Query Frame = 0

Query: 6   REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ--------- 65
           ++GGSS NP+ I       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ         
Sbjct: 48  KKGGSSYNPETIRVYIPQKREIKVGDKVAGRHGNKGIVSKILPRQDMPYLQDGRPVDMVF 107

Query: 66  ------------------------------------------------------------ 125
                                                                       
Sbjct: 108 NPLGVPSRMNVGQIFECSLGLAGELLDRHYRIAPFDERYEQEASRKLVFSELYEASKQTA 167

Query: 126 --------------------------------------------KTNVRSCGHYVLVTQQ 185
                                                       K + R  GHY LVTQQ
Sbjct: 168 NPWVFEPEYPGKSRIFDGRTGDPFEQPVIIGKPYILKLIHQVDDKIHGRCSGHYALVTQQ 227

Query: 186 PLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS----LEEEYLNLRILQNLF--- 192
           PL GRAKQG QRV EME   L    +  + ++ L  +S      +E L   I+       
Sbjct: 228 PLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIIGGPIPKP 287

BLAST of CmaCh04G019580 vs. NCBI nr
Match: RQO99425.1 (hypothetical protein POPTR_013G142232 [Populus trichocarpa])

HSP 1 Score: 135.2 bits (339), Expect = 6.9e-28
Identity = 98/260 (37.69%), Postives = 113/260 (43.46%), Query Frame = 0

Query: 6   REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ--------- 65
           ++GGSS NP+ I       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ         
Sbjct: 284 KKGGSSYNPETIRVYILQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGAPVDMVF 343

Query: 66  ------------------------------------------------------------ 125
                                                                       
Sbjct: 344 NPLGVPSRMNVGQIFECSLGLAGSLLDRHYRVAPFDERYEQEASRKLVFSELYEAGKQTG 403

Query: 126 --------------------------------------------KTNVRSCGHYVLVTQQ 132
                                                       K + RS GHY LVTQQ
Sbjct: 404 NPWVFEPECPGKSRIFDGRTGDPFEQPVIIGKPYILKLIHQVADKIHGRSSGHYALVTQQ 463

BLAST of CmaCh04G019580 vs. NCBI nr
Match: KAF9825357.1 (hypothetical protein H0E87_005079 [Populus deltoides])

HSP 1 Score: 132.1 bits (331), Expect = 5.8e-27
Identity = 97/260 (37.31%), Postives = 112/260 (43.08%), Query Frame = 0

Query: 6   REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ--------- 65
           ++GGSS NP++I       REIKVGDKVAGRHGN+GI+S+++PREDM YLQ         
Sbjct: 607 KKGGSSYNPEIIRVYISQKREIKVGDKVAGRHGNKGIVSKILPREDMPYLQNGRPVDMVF 666

Query: 66  ------------------------------------------------------------ 125
                                                                       
Sbjct: 667 NPLGVPSRMNVGQIFECSLGLAGSLLDRHYRIVPFDERYEQEASRKLVFSELYEASKQTA 726

Query: 126 --------------------------------------------KTNVRSCGHYVLVTQQ 132
                                                       K + RS GHY LVTQQ
Sbjct: 727 SPWVFEPEYPGKSRIFDGRTGNPFEQPVIIGKPYILKLIHQVDDKIHGRSSGHYALVTQQ 786

BLAST of CmaCh04G019580 vs. NCBI nr
Match: MQM19122.1 (hypothetical protein [Colocasia esculenta])

HSP 1 Score: 110.5 bits (275), Expect = 1.8e-20
Identity = 87/257 (33.85%), Postives = 105/257 (40.86%), Query Frame = 0

Query: 8   GGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ----------- 67
           GGS+ +P+MI       REIKVGDKVAGRHGN+GI+S+ +PR+DM YLQ           
Sbjct: 698 GGSNYHPEMIRVYISQKREIKVGDKVAGRHGNKGIISKNLPRQDMLYLQDGIPIDMVFNP 757

Query: 68  ------------------------------------------------------------ 127
                                                                       
Sbjct: 758 LGVPSRMNVGQIFKCSLGLAGDLLNRYYRIVPFDERYEQEASRKLVFLELYEASKQTKNP 817

Query: 128 ------------------------------------------KTNVRSCGHYVLVTQQPL 136
                                                     K + RS GHY LVTQQPL
Sbjct: 818 WVFEPEYPGKSKIFDGRTGDPFEQPVLTGKSYILKLIHQVDDKIHGRSSGHYALVTQQPL 877

BLAST of CmaCh04G019580 vs. NCBI nr
Match: YP_009584286.1 (RNA polymerase beta' subunit-1 [Aconitum brachypodum] >QBL56308.1 RNA polymerase beta' subunit-1 [Aconitum brachypodum])

HSP 1 Score: 109.8 bits (273), Expect = 3.1e-20
Identity = 100/318 (31.45%), Postives = 130/318 (40.88%), Query Frame = 0

Query: 6    REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ--------- 65
            ++GGSS NP+ I       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ         
Sbjct: 793  KKGGSSYNPETIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGTPVDMVF 852

Query: 66   ------------------------------------------------------------ 125
                                                                        
Sbjct: 853  NPLGVPSRMNVGQIFECSLGLAGDLLDRHYRISPFDERYEQEASRKLVFSELYEASKQTA 912

Query: 126  --------------------------------------------KTNVRSCGHYVLVTQQ 185
                                                        K + RS GHY LVTQQ
Sbjct: 913  NPWVFEPEYPGKSRIFDGRTGDPFEQPVIIGKSYILKLIHQVDDKIHGRSSGHYALVTQQ 972

Query: 186  PLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS----LEEEYLNLRILQNLFNS- 192
            PL GRAKQG QRV EME   L    +  + ++ L  +S      +E L   I+    ++ 
Sbjct: 973  PLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHMRARQEVLGTTIIGGTISNP 1032

BLAST of CmaCh04G019580 vs. NCBI nr
Match: YP_010127486.1 (RNA polymerase beta' subunit [Aconitum flavum] >QPP04722.1 RNA polymerase beta' subunit [Aconitum flavum])

HSP 1 Score: 109.8 bits (273), Expect = 3.1e-20
Identity = 100/318 (31.45%), Postives = 130/318 (40.88%), Query Frame = 0

Query: 6    REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ--------- 65
            ++GGSS NP+ I       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ         
Sbjct: 793  KKGGSSYNPETIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGTPVDMVF 852

Query: 66   ------------------------------------------------------------ 125
                                                                        
Sbjct: 853  NPLGVPSRMNVGQIFECSLGLAGDLLDRHYRISPFDERYEQEASRKLVFSELYEASKQTA 912

Query: 126  --------------------------------------------KTNVRSCGHYVLVTQQ 185
                                                        K + RS GHY LVTQQ
Sbjct: 913  NPWVFEPEYPGKSRIFDGRTGDPFEQPVIIGKSYILKLIHQVDDKIHGRSSGHYALVTQQ 972

Query: 186  PLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS----LEEEYLNLRILQNLFNS- 192
            PL GRAKQG QRV EME   L    +  + ++ L  +S      +E L   I+    ++ 
Sbjct: 973  PLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHMRARQEVLGTTIIGGTISNP 1032

BLAST of CmaCh04G019580 vs. TAIR 10
Match: ATCG00190.1 (RNA polymerase subunit beta )

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-11
Identity = 32/51 (62.75%), Postives = 42/51 (82.35%), Query Frame = 0

Query: 6   REGGSSENPDMI-------REIKVGDKVAGRHGNQGILSQMVPREDMSYLQ 50
           ++GGSS NP++I       REIKVGDKVAGRHGN+GI+S+++PR+DM YLQ
Sbjct: 795 KKGGSSYNPEIIRVYISQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQ 845


HSP 2 Score: 48.5 bits (114), Expect = 7.9e-06
Identity = 27/54 (50.00%), Postives = 34/54 (62.96%), Query Frame = 0

Query: 50   KTNVRSCGHYVLVTQQPLIGRAKQGRQRVEEMERCLLINLIILELGKKYLVLRS 104
            K + RS GHY LVTQQPL GR+KQG QRV EME   L    +  + ++ L  +S
Sbjct: 959  KIHGRSSGHYALVTQQPLRGRSKQGGQRVGEMEVWALEGFGVAHILQEMLTYKS 1012

BLAST of CmaCh04G019580 vs. TAIR 10
Match: ATCG00180.1 (DNA-directed RNA polymerase family protein )

HSP 1 Score: 61.6 bits (148), Expect = 9.0e-10
Identity = 28/34 (82.35%), Postives = 29/34 (85.29%), Query Frame = 0

Query: 158 PIAKNPTFVRSRGSFEYEIQSGKYSIPLFVTTQG 192
           PI K PTF+R RGSFEYEIQS KYSIPLF TTQG
Sbjct: 150 PITKKPTFLRLRGSFEYEIQSWKYSIPLFFTTQG 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7Y3B91.0e-1064.71DNA-directed RNA polymerase subunit beta OS=Ipomoea purpurea OX=4121 GN=rpoB PE=... [more]
Q0G9M71.0e-1064.71DNA-directed RNA polymerase subunit beta OS=Liriodendron tulipifera OX=3415 GN=r... [more]
Q68S141.4e-1064.71DNA-directed RNA polymerase subunit beta OS=Panax ginseng OX=4054 GN=rpoB PE=3 S... [more]
Q06H052.3e-1067.35DNA-directed RNA polymerase subunit beta OS=Drimys granadensis OX=224735 GN=rpoB... [more]
P505463.0e-1062.75DNA-directed RNA polymerase subunit beta OS=Arabidopsis thaliana OX=3702 GN=rpoB... [more]
Match NameE-valueIdentityDescription
A0A3N7FZF83.3e-2837.69DNA-directed RNA polymerase OS=Populus trichocarpa OX=3694 GN=POPTR_013G142232 P... [more]
A0A803N3201.9e-2336.44Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1[more]
A0A482AWY81.5e-2031.45DNA-directed RNA polymerase subunit beta OS=Aconitum brachypodum OX=226103 GN=rp... [more]
A0A7T1WQB91.5e-2031.45RNA polymerase beta' subunit OS=Aconitum flavum OX=286045 GN=rpoC1 PE=4 SV=1[more]
A0A7J6EJ594.1e-1831.19Multifunctional fusion protein OS=Cannabis sativa OX=3483 GN=G4B88_017242 PE=3 S... [more]
Match NameE-valueIdentityDescription
RQO99425.16.9e-2837.69hypothetical protein POPTR_013G142232 [Populus trichocarpa][more]
KAF9825357.15.8e-2737.31hypothetical protein H0E87_005079 [Populus deltoides][more]
MQM19122.11.8e-2033.85hypothetical protein [Colocasia esculenta][more]
YP_009584286.13.1e-2031.45RNA polymerase beta' subunit-1 [Aconitum brachypodum] >QBL56308.1 RNA polymerase... [more]
YP_010127486.13.1e-2031.45RNA polymerase beta' subunit [Aconitum flavum] >QPP04722.1 RNA polymerase beta' ... [more]
Match NameE-valueIdentityDescription
ATCG00190.12.1e-1162.75RNA polymerase subunit beta [more]
ATCG00180.19.0e-1082.35DNA-directed RNA polymerase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037033DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamilyGENE3D2.40.270.10coord: 52..80
e-value: 4.1E-5
score: 25.0
IPR037033DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamilyGENE3D2.40.270.10coord: 11..51
e-value: 2.0E-10
score: 42.7
IPR007120DNA-directed RNA polymerase, subunit 2, hybrid-binding domainPFAMPF00562RNA_pol_Rpb2_6coord: 17..50
e-value: 1.9E-10
score: 40.4
IPR015712DNA-directed RNA polymerase, subunit 2PANTHERPTHR20856DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2coord: 53..82
IPR015712DNA-directed RNA polymerase, subunit 2PANTHERPTHR20856DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2coord: 17..50
NoneNo IPR availablePANTHERPTHR20856:SF20DNA-DIRECTED RNA POLYMERASE SUBUNIT BETAcoord: 17..50
NoneNo IPR availablePANTHERPTHR20856:SF20DNA-DIRECTED RNA POLYMERASE SUBUNIT BETAcoord: 53..82
NoneNo IPR availableSUPERFAMILY64484beta and beta-prime subunits of DNA dependent RNA-polymerasecoord: 17..84

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G019580.1CmaCh04G019580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0032549 ribonucleoside binding