CmaCh04G016930 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G016930
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	t-SNARE coiled-coil homology domain-containing protein
Location	Cma_Chr04: 8513095 .. 8516739 (-)
RNA-Seq Expression	CmaCh04G016930
Synteny	CmaCh04G016930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGTACAGTTCTTCTGCTTTTCCGCGTCGCCTCCCGTCAATCTGTTACGGATTCTCAATTTTATCTGTGTTTAAGGATCGTACACGCTGATAATCGTCAAGAACAAAAACCACAATTTCGCTTGCGTTTCATATTTTCTTCCTGCTTCGGATTTCCTCTTCCAATTTTCAGGTACACTCATTTTCCCCTTCTCGATGGTTTTCGTTGAACGGTCTATTTCCAGTTCATTGCTTTGGTGATTCATCTTTTTGTTGAACTTATATTCGTATTTACACTGCATTTCTGTGATTTTTCTCTCCTTTTGAACCCTCATTTTCCGTTCTTATGTTTTATCTGTATCGTTATCGGTTAATTGCTAGGAATATCGGTTGTCGCTTAGCTTTCGATCCCTAATTCTACGTTTGAATTGCATTCTGCATTCTTATTTCCAGTTGCTTTTTGTTAGTCTTTATATGGTAGAATGGTAGTGTTATTTTGTATTGTTACTGCGAATCTATTGTCCTCCCGGAGTCCTGTTTCTATCGAAATTGTTGGCATTACGGCCTTTTTCCTAAATATTCTCACTACTATTTTACAGGTACGAGTTATATTCATCAGATCCTTGTTTGAGAGCCGCTTACCGGATAGTTTATTAGAATTCAAGAATTTCTTTGTAGAGCCAGGATGTTTAGTTTCATGAAATCCCCCGCGAAAGTTACTAAGCAGAATTCTGTAGACGAATTATCAGGCGGATCTGGCACTAATCCTTTTGATTCAGATTCTGGGCCTGAAGCCAAACAAACTCTTAATGCTGCAAGAAGAACTTCTTCTGAGCCTGTGCTCGTTATACCTAACTCCATTTCCGCCAACGCTTTTGATGATGATGATGATGATGGATTTGTTGGAAAAAGAGGGACTGCAACTTCTGCAGCCTCTAAAGACAGGTACAAAAATGATTTTCGTGACTCAGGAGGACTCGAGAACCAAAGTGTGCAGGAATTGGAGAATTATGCTCTGTATAAAGCAGAGGAGACCACAAAATCTGTTAACAATTGCTTGAAGATTGCTGAGGACATAAGGGAAGATGCTACCAAGACCCTTGACATGTTGCATCAGCAGGGTGAGCAAATTGAGAGGACCCACAGGATGGCTGCTGATATGGATAAAGATTTAAGCAAGGTAAGCTCTTTTCGAGTTTTGATCAGACACCTTGCGGATGTATTTCTATCCTATGTTGTTATTCCTGAAATTATTAGTTGGCTCATGTTGATCTGATATAATTAAAAACAGAATCAGATTATTCAAAACTTTAAAGTTAGCAGTTATGAAATATTTGGCTTGCAAGTTACGATGCGATTAGGTTGTGGATGTCCTACTCTGTAAGTGATGGAAGATGTTCGAGAGCAAAGAACCTTTTTGTTTGCTAGGAATTTGTTAAGCAAGCCAGAAATGATTGAGGTTTTCACTTGCTTTTTCGTTTTTTATGTATGCATTTTTTATGATCCTTTGATTGCTCCTCCATGTGTTGTCAATGGTTTCTTATTCATTATTGTTAATGACAGGGTGAGAAACTTCTGAATAATCTGGGAGGCATGTTCTCAAAGCCATGGAAGCCAAAGAAGACCAAAGAAATTACAGGCCCTCTTATAACAGCAGGTTATATTTTCACTGGTTCTTGTAACTTACTGCCTCTTCCATTTTATCTGGCTATTAATTATGTCGGCTGTTCAGATCATTCCTCTGGGCGAACTGAAAACAACAAGGAGCAAAGGGAAAAACTCGGTGTATCTACGGGCAAAAAACAGTCGGCGACTAAAACACCACCCTCTGAACCAACAGGTGCAATACAAAAAGTTGAGGTGAGAGCGTCGGCCGTTATCATTTCACTATCTTAAAGAGTATAACATTATCATGGATTCAAATTTAACCAATATCTAAGAATGTTAACTCAGGCTCAGAAGGAAATGCAAGATGATGCACTCTCAGATTTAAGTAATATCTTGGGTGATCTGAAGAGTATGGCTGTGGACATGGGAAGCGAGCTTGACAGGTCATACATGTCTACCTCTTTTCTCAATCTAGATATTGTTGTATTATAACTCATCAATCTGTTGGCTTATTACATAGTTCTGAATGGTTACTACTCTCTGGAAAATGCAGGCAAAACAAAGCTCTTGATCATCTAAGCGACGATGTCGACGAGCTGAATTCTAGAGTGAAGGGTGCCAATCAGCGGGCTCGTCATTTGGTTGGGAAGTAAATCCTGCCTCTTCCTTCTGTTTGTTTCTAGCTGGAAGCATTTCGATCAAGCATTCTTTAATTGTTGGTATACTATTATTTTCTCATGTGGTTTTATAAGGTTGCAATAAGTACACAACTTTGATTCTATTCTTTCAATGAAAAAGCTTATCCCTTCATCTTGATGTAGATAGATACTAGGAGCTGAACATTAGCTGCTAACGTTTTCTTGACTAAGCTAATGGAAGATAAAGCCAGTTCAAAAGAGAAAAGGGAAGAAGTAAAAGAAACTCAATTGGGGCTTTTACATACATAGATTTCCACAGATTCTAGCAAAATTACATTAACTCCATAAACAGTAAACTAGAAATGAAAGTTCCATAGTGCCCCTCCCAATGAAGGAAACCTCATGAATCCTGCTCTGTTGACATTTTGTACCTTATGCCTCCAAAATTAATGAGACTCATGGGTTTCTTTTGAATCTCGCCTCCGAACCCCGACACCTATGACCAAATTCTTTTTTAAGTGTATAAAAAATCCAAATCTTAAAGTAAATACCTTGCCATTTCCATGCTTGTTTTTGCCAGTTTTCACATCAAAATGAACTCCAACGTTCGCCTCCTCTCAATGCTCGAACTTTTAAGCCACCAAAACTCGAACGCTTCGACGCAAAAACAAGAATGGAAATAGCAACAATAACCTGAAACAAGAAAGAAAAACAAGACAAGATGACATTGGACAAAACTTGTCAACTAATTAGCGTAGCCTTCTTCTGCCTTTGGTCATGCACGAAGATTAAATATTCTTCTTGATCCTTAACTGTAATAGGACTCAAGAAAAAAAAGGGACGTGCATGATATACTACTGGAATAATAGCAAGAAAACTGAGAAGGGAAAGGGATAAAATGGCTGTGTTCTGCTAAAAAAAAGATTCTTTTTGCTCGTTCTGTGCTTTCTTTTTCTCTTGTTCAGTTGTGAAGAGGGTTTGGCCCCGAGGGCACGAGTCTCTTCTCGACGCCATATCGGGGGTCAATCTCATTGTGTGTCAGCATCGGCGGGACATGAACAGGTAGGTGGTGTTGGTAAACTCTATAAAGGTGAATACACAAAGAACGTGAGTTTTCCTGGGTTAGAGCATCACAAGGATGAAGCTGCTGCAGTTCACGGTGAGGCAGTGGGTGGCTAGAGGTGGTCGGAGAAGCGTCTGGCACATGGGTGGAAGGTGGATAACCGGAGAGTCTGGTGGCAGATGAGTTGACAAAGACGAGTAGAAGCATCGATATCATCATGAAGAGCTGGGCATTGGTGACAGAAGACGATGTACAGGGGGGAGAGAGGGAGGCTTTCATTGTCGAGTTCTGATAGAGAGAGAGAAGGGCGGGGAAGAAAGGAGATAAAAGAGAGGAGATGGTGATAGGGAAAGGGAAG

mRNA sequence

GCGTACAGTTCTTCTGCTTTTCCGCGTCGCCTCCCGTCAATCTGTTACGGATTCTCAATTTTATCTGTGTTTAAGGATCGTACACGCTGATAATCGTCAAGAACAAAAACCACAATTTCGCTTGCGTTTCATATTTTCTTCCTGCTTCGGATTTCCTCTTCCAATTTTCAGGTACGAGTTATATTCATCAGATCCTTGTTTGAGAGCCGCTTACCGGATAGTTTATTAGAATTCAAGAATTTCTTTGTAGAGCCAGGATGTTTAGTTTCATGAAATCCCCCGCGAAAGTTACTAAGCAGAATTCTGTAGACGAATTATCAGGCGGATCTGGCACTAATCCTTTTGATTCAGATTCTGGGCCTGAAGCCAAACAAACTCTTAATGCTGCAAGAAGAACTTCTTCTGAGCCTGTGCTCGTTATACCTAACTCCATTTCCGCCAACGCTTTTGATGATGATGATGATGATGGATTTGTTGGAAAAAGAGGGACTGCAACTTCTGCAGCCTCTAAAGACAGGTACAAAAATGATTTTCGTGACTCAGGAGGACTCGAGAACCAAAGTGTGCAGGAATTGGAGAATTATGCTCTGTATAAAGCAGAGGAGACCACAAAATCTGTTAACAATTGCTTGAAGATTGCTGAGGACATAAGGGAAGATGCTACCAAGACCCTTGACATGTTGCATCAGCAGGGTGAGCAAATTGAGAGGACCCACAGGATGGCTGCTGATATGGATAAAGATTTAAGCAAGGGTGAGAAACTTCTGAATAATCTGGGAGGCATGTTCTCAAAGCCATGGAAGCCAAAGAAGACCAAAGAAATTACAGGCCCTCTTATAACAGCAGATCATTCCTCTGGGCGAACTGAAAACAACAAGGAGCAAAGGGAAAAACTCGGTGTATCTACGGGCAAAAAACAGTCGGCGACTAAAACACCACCCTCTGAACCAACAGGTGCAATACAAAAAGTTGAGGCTCAGAAGGAAATGCAAGATGATGCACTCTCAGATTTAAGTAATATCTTGGGTGATCTGAAGAGTATGGCTGTGGACATGGGAAGCGAGCTTGACAGGCAAAACAAAGCTCTTGATCATCTAAGCGACGATGTCGACGAGCTGAATTCTAGAGTGAAGGGTGCCAATCAGCGGGCTCGTCATTTGGTTGGGAAGTAAATCCTGCCTCTTCCTTCTGTTTGTTTCTAGCTGGAAGCATTTCGATCAAGCATTCTTTAATTGTTGGTATACTATTATTTTCTCATGTGGTTTTATAAGGTTGCAATAAGTACACAACTTTGATTCTATTCTTTCAATGAAAAAGCTTATCCCTTCATCTTGATGTAGATAGATACTAGGAGCTGAACATTAGCTGCTAACGTTTTCTTGACTAAGCTAATGGAAGATAAAGCCAGTTCAAAAGAGAAAAGGGAAGAAGTAAAAGAAACTCAATTGGGGCTTTTACATACATAGATTTCCACAGATTCTAGCAAAATTACATTAACTCCATAAACAGTAAACTAGAAATGAAAGTTCCATAGTGCCCCTCCCAATGAAGGAAACCTCATGAATCCTGCTCTGTTGACATTTTGTACCTTATGCCTCCAAAATTAATGAGACTCATGGGTTTCTTTTGAATCTCGCCTCCGAACCCCGACACCTATGACCAAATTCTTTTTTAAGTGTATAAAAAATCCAAATCTTAAAGTAAATACCTTGCCATTTCCATGCTTGTTTTTGCCAGTTTTCACATCAAAATGAACTCCAACGTTCGCCTCCTCTCAATGCTCGAACTTTTAAGCCACCAAAACTCGAACGCTTCGACGCAAAAACAAGAATGGAAATAGCAACAATAACCTGAAACAAGAAAGAAAAACAAGACAAGATGACATTGGACAAAACTTGTCAACTAATTAGCGTAGCCTTCTTCTGCCTTTGGTCATGCACGAAGATTAAATATTCTTCTTGATCCTTAACTGTAATAGGACTCAAGAAAAAAAAGGGACGTGCATGATATACTACTGGAATAATAGCAAGAAAACTGAGAAGGGAAAGGGATAAAATGGCTGTGTTCTGCTAAAAAAAAGATTCTTTTTGCTCGTTCTGTGCTTTCTTTTTCTCTTGTTCAGTTGTGAAGAGGGTTTGGCCCCGAGGGCACGAGTCTCTTCTCGACGCCATATCGGGGGTCAATCTCATTGTGTGTCAGCATCGGCGGGACATGAACAGGTAGGTGGTGTTGGTAAACTCTATAAAGGTGAATACACAAAGAACGTGAGTTTTCCTGGGTTAGAGCATCACAAGGATGAAGCTGCTGCAGTTCACGGTGAGGCAGTGGGTGGCTAGAGGTGGTCGGAGAAGCGTCTGGCACATGGGTGGAAGGTGGATAACCGGAGAGTCTGGTGGCAGATGAGTTGACAAAGACGAGTAGAAGCATCGATATCATCATGAAGAGCTGGGCATTGGTGACAGAAGACGATGTACAGGGGGGAGAGAGGGAGGCTTTCATTGTCGAGTTCTGATAGAGAGAGAGAAGGGCGGGGAAGAAAGGAGATAAAAGAGAGGAGATGGTGATAGGGAAAGGGAAG

Coding sequence (CDS)

ATGTTTAGTTTCATGAAATCCCCCGCGAAAGTTACTAAGCAGAATTCTGTAGACGAATTATCAGGCGGATCTGGCACTAATCCTTTTGATTCAGATTCTGGGCCTGAAGCCAAACAAACTCTTAATGCTGCAAGAAGAACTTCTTCTGAGCCTGTGCTCGTTATACCTAACTCCATTTCCGCCAACGCTTTTGATGATGATGATGATGATGGATTTGTTGGAAAAAGAGGGACTGCAACTTCTGCAGCCTCTAAAGACAGGTACAAAAATGATTTTCGTGACTCAGGAGGACTCGAGAACCAAAGTGTGCAGGAATTGGAGAATTATGCTCTGTATAAAGCAGAGGAGACCACAAAATCTGTTAACAATTGCTTGAAGATTGCTGAGGACATAAGGGAAGATGCTACCAAGACCCTTGACATGTTGCATCAGCAGGGTGAGCAAATTGAGAGGACCCACAGGATGGCTGCTGATATGGATAAAGATTTAAGCAAGGGTGAGAAACTTCTGAATAATCTGGGAGGCATGTTCTCAAAGCCATGGAAGCCAAAGAAGACCAAAGAAATTACAGGCCCTCTTATAACAGCAGATCATTCCTCTGGGCGAACTGAAAACAACAAGGAGCAAAGGGAAAAACTCGGTGTATCTACGGGCAAAAAACAGTCGGCGACTAAAACACCACCCTCTGAACCAACAGGTGCAATACAAAAAGTTGAGGCTCAGAAGGAAATGCAAGATGATGCACTCTCAGATTTAAGTAATATCTTGGGTGATCTGAAGAGTATGGCTGTGGACATGGGAAGCGAGCTTGACAGGCAAAACAAAGCTCTTGATCATCTAAGCGACGATGTCGACGAGCTGAATTCTAGAGTGAAGGGTGCCAATCAGCGGGCTCGTCATTTGGTTGGGAAGTAA

Protein sequence

MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSISANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKSVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEAQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARHLVGK

Homology

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BLAST of CmaCh04G016930 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 3.7e-91
Identity = 185/304 (60.86%), Postives = 218/304 (71.71%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60
           MF F KSP                G N   ++S      T+ A RRTSSEP+L+ P+   
Sbjct: 1   MFGFFKSP----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITPD--- 60

Query: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120
              FDDD                  D+YKN F DSGGL++Q+ +ELE YA+YKAEETTK 
Sbjct: 61  ---FDDD------------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKG 120

Query: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIR D  +TL+MLHQQGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240
           WKPKKTK ITGP+IT D  S ++EN+KE+REKLG+   K +S+++    +PT A+QKVE 
Sbjct: 181 WKPKKTKNITGPMITPDKPSKKSENHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQ 240

Query: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300
           +K  QDD LSDLS+ILGDLKSMAVDMGSE+D+QNKALDHL DDVDELNSRV+GANQRARH
Sbjct: 241 EKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARH 263

Query: 301 LVGK 305
           L+ K
Sbjct: 301 LLSK 263

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BLAST of CmaCh04G016930 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.4e-84
Identity = 179/306 (58.50%), Postives = 219/306 (71.57%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLV-IPNSI 60
           MF   KSPA + K NSVD  S  S  NPFDSD   + K TLN ++RT+SEP L  + N  
Sbjct: 1   MFGLRKSPANLPKHNSVDLKS--SKPNPFDSDDESDNKHTLNPSKRTTSEPSLADMTNPF 60

Query: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120
                   D      K+   ++  SK +YKN+FRDSGG+ENQSVQELE YA+YKAEETTK
Sbjct: 61  GGERVQKGDSSS--SKQSLFSN--SKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SV  CLK+AEDIR DAT+TL MLH QGEQI RTH  A ++D DLS+GEKLL +LGGMFSK
Sbjct: 121 SVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSK 180

Query: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKK-QSATKTPPSEPTGAIQKV 240
            WKPKKT+ I GP++T D S  R  N+ E+REKLG+++  + QS T+ P  E   A Q+V
Sbjct: 181 TWKPKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRV 240

Query: 241 EAQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRA 300
           E +K  QDD LSDLS+ILG+LK+MAVDMGSE+++QNK LDHL DDVDELN RV+ +NQR 
Sbjct: 241 EMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRG 300

Query: 301 RHLVGK 305
           R L+GK
Sbjct: 301 RRLLGK 300

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BLAST of CmaCh04G016930 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 8.3e-59
Identity = 131/247 (53.04%), Postives = 172/247 (69.64%), Query Frame = 0


Query: 58  SISANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEET 117
           S S N FDDDDDD  V KR T++            + SGG ENQ+VQELE+YA+Y +EET
Sbjct: 20  SFSLNPFDDDDDDKEVEKRFTSS-----------LKPSGGKENQTVQELESYAVYNSEET 79

Query: 118 TKSVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMF 177
           TK+V  CLK+AE+IR DA+KTL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+F
Sbjct: 80  TKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVF 139

Query: 178 SKPWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQK 237
           S+ WKPKK++ ITGP+IT   S  R   + + REKLG++   K  +   P  E   A QK
Sbjct: 140 SRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKTLP--EAVDAYQK 199

Query: 238 VEAQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQR 297
            +  K  QD+AL+DLS +LG+LK+MAVDMG+ ++RQ   LDHL D+ DELN RVK +NQR
Sbjct: 200 TQIAK--QDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQR 251

Query: 298 ARHLVGK 305
           AR+L+ K
Sbjct: 260 ARYLLRK 251

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BLAST of CmaCh04G016930 vs. ExPASy Swiss-Prot
Match: P36977 (Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.6e-15
Identity = 59/208 (28.37%), Postives = 108/208 (51.92%), Query Frame = 0


Query: 103 VQELENYALYKAEETTKSVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKD 162
           + +++  A   A+E+ +S    L++ E+ ++   +TL ML +QGEQ+ER       ++KD
Sbjct: 11  LSDMQQRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 163 LSKGEKLLNNLG---GMFSKPWKPKKTKEITGPLITADHSSGRTENNK----EQREKLGV 222
           +   EK LN+LG   G+ S P    K+    G     ++  G   +      ++RE++ +
Sbjct: 71  MKDAEKNLNDLGKFCGLCSCPCNKMKS---GGSKAWGNNQDGVVASQPARVVDEREQMAI 130

Query: 223 STGKKQSATKTPPSEPTGAIQKVEAQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNK 282
           S G  +  T              +A++   D+ L  +  I+G+L+ MA+DMG+E+D QN+
Sbjct: 131 SGGFIRRVTD-------------DARENEMDENLEQVGGIIGNLRHMALDMGNEIDTQNR 190

Query: 283 ALDHLSDDVDELNSRVKGANQRARHLVG 304
            +D + +  D   +R+  ANQRA  ++G
Sbjct: 191 QIDRIMEKADSNKTRIDEANQRATKMLG 202

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BLAST of CmaCh04G016930 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.6e-14
Identity = 57/207 (27.54%), Postives = 108/207 (52.17%), Query Frame = 0


Query: 103 VQELENYALYKAEETTKSVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKD 162
           ++E++  A   A+E+ +S    L++ E+ ++   +TL ML +QGEQ+ER       ++KD
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 163 LSKGEKLLNNLG---GMFSKPWKPKKTKEITGPLITADHS---SGRTENNKEQREKLGVS 222
           + + EK L +LG   G+   P    K+ +        +     + +     ++RE++ +S
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAIS 130

Query: 223 TGKKQSATKTPPSEPTGAIQKVEAQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKA 282
            G  +  T              +A++   D+ L  +S I+G+L+ MA+DMG+E+D QN+ 
Sbjct: 131 GGFIRRVTN-------------DARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQ 190

Query: 283 LDHLSDDVDELNSRVKGANQRARHLVG 304
           +D + +  D   +R+  ANQRA  ++G
Sbjct: 191 IDRIMEKADSNKTRIDEANQRATKMLG 204

[Close]

BLAST of CmaCh04G016930 vs. ExPASy TrEMBL
Match: A0A6J1J9F6 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111482502 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60
           MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS
Sbjct: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60

Query: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120
           ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS
Sbjct: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240
           WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA
Sbjct: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240

Query: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300
           QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH
Sbjct: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300

Query: 301 LVGK 305
           LVGK
Sbjct: 301 LVGK 304

[Close]

BLAST of CmaCh04G016930 vs. ExPASy TrEMBL
Match: A0A6J1H000 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111458786 PE=4 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 5.6e-159
Identity = 297/304 (97.70%), Postives = 301/304 (99.01%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60
           MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVL+IPNSIS
Sbjct: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLIIPNSIS 60

Query: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120
           AN FDDDDDDGFVGKR TATSAASKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTKS
Sbjct: 61  ANDFDDDDDDGFVGKRRTATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           V+NCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VHNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240
           WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQS TKTPPSEPTGAIQKVEA
Sbjct: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQKVEA 240

Query: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300
           QKEMQDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH
Sbjct: 241 QKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300

Query: 301 LVGK 305
           LVGK
Sbjct: 301 LVGK 304

[Close]

BLAST of CmaCh04G016930 vs. ExPASy TrEMBL
Match: A0A0A0KQT5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G606590 PE=4 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.5e-148
Identity = 280/305 (91.80%), Postives = 293/305 (96.07%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60
           MFSFMKSPAKVTKQNSVD ELS GSGTNPFDSD+GP+AKQTLNAARRTSSEPVL +P   
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMP--- 60

Query: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120
            AN FDDDDD GFVG++GTATS+ SKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTK
Sbjct: 61  KANPFDDDDDTGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDATKTLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVE 240
           PWKPKKTKEITGPLITADHSSG+TENNKEQREKLG+STGKKQSATKTPPSEP+GAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATKTPPSEPSGAIQKVE 240

Query: 241 AQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
            +KE QDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 HLVGK 305
           HL+GK
Sbjct: 301 HLIGK 302

[Close]

BLAST of CmaCh04G016930 vs. ExPASy TrEMBL
Match: A0A1S3BF13 (putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC103489173 PE=4 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 8.4e-147
Identity = 279/305 (91.48%), Postives = 293/305 (96.07%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60
           MFSFMKSPAKVTKQNSVD ELS GSGTNPFDSD+GP+AKQTLNAARRTSSEPVL IPN  
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIPN-- 60

Query: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120
            AN F  DDDDGFVG++GTATS+ SKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTK
Sbjct: 61  -ANPF--DDDDGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDATKTLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVE 240
           PWKPKKTKEITGPLITADHSSG+TENNK+QREKLG+STGKKQSATKTPPSEP+GA+QKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGKTENNKKQREKLGLSTGKKQSATKTPPSEPSGAMQKVE 240

Query: 241 AQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
            +KE QDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 HLVGK 305
           HL+GK
Sbjct: 301 HLIGK 300

[Close]

BLAST of CmaCh04G016930 vs. ExPASy TrEMBL
Match: A0A5D3CF89 (Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00020 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.6e-140
Identity = 277/340 (81.47%), Postives = 292/340 (85.88%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60
           MFSFMKSPAKVTKQNSVD ELS GSGTNPFDSD+GP+AKQTLNAARRTSSEPVL IPN  
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIPN-- 60

Query: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120
            AN F  DDDDGFVG++GTATS+ SKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTK
Sbjct: 61  -ANPF--DDDDGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLS--------------- 180
           SVNNCLKIAEDIREDATKTLDMLH+QGEQIERTHRMAADMDKDL+               
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLNIFGLQVFVMIRLWMS 180

Query: 181 --------------------KGEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGRTE 240
                                GEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSG+TE
Sbjct: 181 YSVGLKLVKNEQPFSLLGILYGEKLLNNLGGMFSKPWKPKKTKEITGPLITADHSSGKTE 240

Query: 241 NNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEAQKEMQDDALSDLSNILGDLKSMAV 300
           NNK+QREKLG+STGKKQSATKTPPSEP+GA+QKVE +KE QDDALSDLSNILGDLKSMAV
Sbjct: 241 NNKKQREKLGLSTGKKQSATKTPPSEPSGAMQKVEVEKEKQDDALSDLSNILGDLKSMAV 300

Query: 301 DMGSELDRQNKALDHLSDDVDELNSRVKGANQRARHLVGK 305
           DMGSELDRQNKALDHLSDDVDELNSRVKGANQRARHL+GK
Sbjct: 301 DMGSELDRQNKALDHLSDDVDELNSRVKGANQRARHLIGK 335

[Close]

BLAST of CmaCh04G016930 vs. NCBI nr
Match: XP_022984084.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita maxima])

HSP 1 Score: 582.4 bits (1500), Expect = 2.3e-162
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60
           MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS
Sbjct: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60

Query: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120
           ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS
Sbjct: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240
           WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA
Sbjct: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240

Query: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300
           QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH
Sbjct: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300

Query: 301 LVGK 305
           LVGK
Sbjct: 301 LVGK 304

[Close]

BLAST of CmaCh04G016930 vs. NCBI nr
Match: XP_023528612.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.0 bits (1473), Expect = 3.0e-159
Identity = 298/304 (98.03%), Postives = 300/304 (98.68%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60
           MFSFMKSPAKVTKQNSVDEL GGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS
Sbjct: 1   MFSFMKSPAKVTKQNSVDELPGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60

Query: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120
           ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTKS
Sbjct: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIREDA KTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDAAKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240
           WKPKKTKEITGPLITADHSSGR ENNKEQREKLGVSTGKKQS TKTPPSEPTGAIQKVEA
Sbjct: 181 WKPKKTKEITGPLITADHSSGRAENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQKVEA 240

Query: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300
           QKEMQDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH
Sbjct: 241 QKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300

Query: 301 LVGK 305
           LVGK
Sbjct: 301 LVGK 304

[Close]

BLAST of CmaCh04G016930 vs. NCBI nr
Match: XP_022957363.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >KAG7032248.1 putative SNAP25-likeous protein SNAP30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.1 bits (1468), Expect = 1.2e-158
Identity = 297/304 (97.70%), Postives = 301/304 (99.01%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60
           MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVL+IPNSIS
Sbjct: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLIIPNSIS 60

Query: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120
           AN FDDDDDDGFVGKR TATSAASKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTKS
Sbjct: 61  ANDFDDDDDDGFVGKRRTATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           V+NCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VHNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240
           WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQS TKTPPSEPTGAIQKVEA
Sbjct: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQKVEA 240

Query: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300
           QKEMQDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH
Sbjct: 241 QKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300

Query: 301 LVGK 305
           LVGK
Sbjct: 301 LVGK 304

[Close]

BLAST of CmaCh04G016930 vs. NCBI nr
Match: KAG6601467.1 (putative SNAP25-likeous protein SNAP30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 568.5 bits (1464), Expect = 3.4e-158
Identity = 297/304 (97.70%), Postives = 300/304 (98.68%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60
           MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS
Sbjct: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60

Query: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120
           AN FDDDDDDGFVGKR  ATSAASKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTKS
Sbjct: 61  ANDFDDDDDDGFVGKRRAATSAASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           V+NCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VHNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240
           WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQS TKTPPSEPTGAIQKVEA
Sbjct: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSGTKTPPSEPTGAIQKVEA 240

Query: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300
           QKEMQDDALSDLS+ILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH
Sbjct: 241 QKEMQDDALSDLSDILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300

Query: 301 LVGK 305
           LVGK
Sbjct: 301 LVGK 304

[Close]

BLAST of CmaCh04G016930 vs. NCBI nr
Match: XP_004135151.1 (putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_011655703.1 putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_031740914.1 putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >KGN51968.1 hypothetical protein Csa_009347 [Cucumis sativus])

HSP 1 Score: 535.4 bits (1378), Expect = 3.2e-148
Identity = 280/305 (91.80%), Postives = 293/305 (96.07%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVD-ELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60
           MFSFMKSPAKVTKQNSVD ELS GSGTNPFDSD+GP+AKQTLNAARRTSSEPVL +P   
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMP--- 60

Query: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120
            AN FDDDDD GFVG++GTATS+ SKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTK
Sbjct: 61  KANPFDDDDDTGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIREDATKTLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVE 240
           PWKPKKTKEITGPLITADHSSG+TENNKEQREKLG+STGKKQSATKTPPSEP+GAIQKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATKTPPSEPSGAIQKVE 240

Query: 241 AQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
            +KE QDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 HLVGK 305
           HL+GK
Sbjct: 301 HLIGK 302

[Close]

BLAST of CmaCh04G016930 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 336.3 bits (861), Expect = 2.6e-92
Identity = 185/304 (60.86%), Postives = 218/304 (71.71%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSIS 60
           MF F KSP                G N   ++S      T+ A RRTSSEP+L+ P+   
Sbjct: 1   MFGFFKSP----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITPD--- 60

Query: 61  ANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTKS 120
              FDDD                  D+YKN F DSGGL++Q+ +ELE YA+YKAEETTK 
Sbjct: 61  ---FDDD------------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKG 120

Query: 121 VNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIR D  +TL+MLHQQGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVEA 240
           WKPKKTK ITGP+IT D  S ++EN+KE+REKLG+   K +S+++    +PT A+QKVE 
Sbjct: 181 WKPKKTKNITGPMITPDKPSKKSENHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQ 240

Query: 241 QKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 300
           +K  QDD LSDLS+ILGDLKSMAVDMGSE+D+QNKALDHL DDVDELNSRV+GANQRARH
Sbjct: 241 EKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARH 263

Query: 301 LVGK 305
           L+ K
Sbjct: 301 LLSK 263

[Close]

BLAST of CmaCh04G016930 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 312.8 bits (800), Expect = 3.1e-85
Identity = 179/306 (58.50%), Postives = 219/306 (71.57%), Query Frame = 0


Query: 1   MFSFMKSPAKVTKQNSVDELSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLV-IPNSI 60
           MF   KSPA + K NSVD  S  S  NPFDSD   + K TLN ++RT+SEP L  + N  
Sbjct: 1   MFGLRKSPANLPKHNSVDLKS--SKPNPFDSDDESDNKHTLNPSKRTTSEPSLADMTNPF 60

Query: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120
                   D      K+   ++  SK +YKN+FRDSGG+ENQSVQELE YA+YKAEETTK
Sbjct: 61  GGERVQKGDSSS--SKQSLFSN--SKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SV  CLK+AEDIR DAT+TL MLH QGEQI RTH  A ++D DLS+GEKLL +LGGMFSK
Sbjct: 121 SVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSK 180

Query: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKK-QSATKTPPSEPTGAIQKV 240
            WKPKKT+ I GP++T D S  R  N+ E+REKLG+++  + QS T+ P  E   A Q+V
Sbjct: 181 TWKPKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRV 240

Query: 241 EAQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRA 300
           E +K  QDD LSDLS+ILG+LK+MAVDMGSE+++QNK LDHL DDVDELN RV+ +NQR 
Sbjct: 241 EMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRG 300

Query: 301 RHLVGK 305
           R L+GK
Sbjct: 301 RRLLGK 300

[Close]

BLAST of CmaCh04G016930 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 228.8 bits (582), Expect = 5.9e-60
Identity = 131/247 (53.04%), Postives = 172/247 (69.64%), Query Frame = 0


Query: 58  SISANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEET 117
           S S N FDDDDDD  V KR T++            + SGG ENQ+VQELE+YA+Y +EET
Sbjct: 20  SFSLNPFDDDDDDKEVEKRFTSS-----------LKPSGGKENQTVQELESYAVYNSEET 79

Query: 118 TKSVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMF 177
           TK+V  CLK+AE+IR DA+KTL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+F
Sbjct: 80  TKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVF 139

Query: 178 SKPWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQK 237
           S+ WKPKK++ ITGP+IT   S  R   + + REKLG++   K  +   P  E   A QK
Sbjct: 140 SRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKTLP--EAVDAYQK 199

Query: 238 VEAQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQR 297
            +  K  QD+AL+DLS +LG+LK+MAVDMG+ ++RQ   LDHL D+ DELN RVK +NQR
Sbjct: 200 TQIAK--QDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQR 251

Query: 298 ARHLVGK 305
           AR+L+ K
Sbjct: 260 ARYLLRK 251

The following BLAST results are available for this feature:

BLAST of CmaCh04G016930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMG8	3.7e-91	60.86	Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA...	[more]
Q9S7P9	4.4e-84	58.50	SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ...	[more]
Q9SD96	8.3e-59	53.04	SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ...	[more]
P36977	5.6e-15	28.37	Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=...	[more]
Q17QQ3	1.6e-14	27.54	Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1	[more]

BLAST of CmaCh04G016930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J9F6	1.1e-162	100.00	putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1H000	5.6e-159	97.70	putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A0A0KQT5	1.5e-148	91.80	t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365...	[more]
A0A1S3BF13	8.4e-147	91.48	putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC10348917...	[more]
A0A5D3CF89	2.6e-140	81.47	Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=11...	[more]

BLAST of CmaCh04G016930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022984084.1	2.3e-162	100.00	putative SNAP25 homologous protein SNAP30 [Cucurbita maxima]	[more]
XP_023528612.1	3.0e-159	98.03	putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo]	[more]
XP_022957363.1	1.2e-158	97.70	putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >KAG7032248.1 put...	[more]
KAG6601467.1	3.4e-158	97.70	putative SNAP25-likeous protein SNAP30, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_004135151.1	3.2e-148	91.80	putative SNAP25 homologous protein SNAP30 [Cucumis sativus] >XP_011655703.1 puta...	[more]

BLAST of CmaCh04G016930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13890.1	2.6e-92	60.86	soluble N-ethylmaleimide-sensitive factor adaptor protein 30	[more]
AT5G61210.1	3.1e-85	58.50	soluble N-ethylmaleimide-sensitive factor adaptor protein 33	[more]
AT5G07880.1	5.9e-60	53.04	synaptosomal-associated protein SNAP25-like 29	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 125..145
None	No IPR available	COILS	Coil	Coil	coord: 270..297
None	No IPR available	GENE3D	1.20.5.110		coord: 236..303 e-value: 1.4E-22 score: 81.7
None	No IPR available	GENE3D	1.20.5.110		coord: 97..175 e-value: 7.8E-22 score: 79.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 174..234
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..56
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 216..231
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..85
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 201..215
None	No IPR available	PANTHER	PTHR19305	SYNAPTOSOMAL ASSOCIATED PROTEIN	coord: 1..304
None	No IPR available	PANTHER	PTHR19305:SF25	SNAP25 HOMOLOGOUS PROTEIN SNAP30-RELATED	coord: 1..304
None	No IPR available	CDD	cd15841	SNARE_Qc	coord: 242..300 e-value: 2.37773E-15 score: 67.1984
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 234..301
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 101..172
IPR000727	Target SNARE coiled-coil homology domain	SMART	SM00397	tSNARE_6	coord: 234..301 e-value: 1.0E-9 score: 48.3 coord: 106..173 e-value: 0.023 score: 23.9
IPR000727	Target SNARE coiled-coil homology domain	PROSITE	PS50192	T_SNARE	coord: 239..301 score: 17.107162
IPR044766	NPSN/SNAP25-like, N-terminal SNARE domain	CDD	cd15861	SNARE_SNAP25N_23N_29N_SEC9N	coord: 108..172 e-value: 3.4105E-21 score: 83.3915

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G016930.1	CmaCh04G016930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061025	membrane fusion
biological_process	GO:0015031	protein transport
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005484	SNAP receptor activity