CmaCh04G016300 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G016300
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein TWIN LOV 1
LocationCma_Chr04: 8216817 .. 8220606 (-)
RNA-Seq ExpressionCmaCh04G016300
SyntenyCmaCh04G016300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCGTCGGTGCCCTCGTTTGCTTCTGTTCTCAGCCCATCAATACATTGTCCCTCATCTACGTGGACACTTCTTGTCACAATGATTGGGACTACCATCGGCACACCCTCTCTAATTCCTCCGTTTTTCGGTAAATCTGTGGCAACTCAGCCAGAAAATTATACCTTGATCTTCATGATTTACATTCTTGTTATCTTTTTGATATGATCAGAAGCTTAGAACAATGGGATAACCATTTTTCCTTGTTTTTTTTGTAAATATTTTGTGGCCAAAGGAGGGGGTAATATCAACCAGAAATATTGTGCCGCGAATTTGTGAACGGCGTTGGTTCTGCTTTCTTTTTCTTCATTGCTGGTTTGATATTTTTCATTTTCCAATCTCGACGGCGTGATGGGTGATTTGATTTCTGGATCATTTTCATTTCAATTTTCTCATCGCATCGAAATATGGATAGTTGCGAACAATTTTTGTTGATCACAATTTACGCGATATTTTTCATTCGGATGTTCTCTTATTCACAGTCATATGGACACTCGTAGTTGCTAAGTGTGTTCTCCCCGCGAGTAGCTTGTCGAATCCCTGAGTCGATACGGATTCATTCTAGGGTTTTTGTTTGTTGTTCTTCTTGATTTTATATACTGAAGTTCAAAGCTTCTTCGAGAGACTGAACCAGAGGTTTGAGTGCCTCATGGGATTGGGACTATTCCGAATATCGAGGAAGAAAGGGGTTTTAGTCCAACAAGTTGAAGAACCAGGAGGATGGAATTTCAATTGGGTTTGATTGAACAATCACTTAACAGTCGTTATTCGCTCTGGGTCCGTGAAGCTCTTAATGATTTGTCGGATAATTTTACAATCACTGATCCCAGTATGTCGGGTCACCCAATTGTGTTCGTGAGCCGTGGATTCTTGAAGATGACTGGATATACCAAAGAAGAGGTGATTGGAAAAAATGGGAGGATGTTTCAGGGCCCAAAAACTTGTAGGAGTTCAGTAATGCAAATTCGCGAGGCAATTCGGGAAGAGAAGGGGATTCAAATTAATTTGCTAAATTATCGTAAAGATGGTACACCCTTCTGGGTCTTTTTTCAACTGAGCCCCGTCTTCTGCAAGGAAGATGGGCGGATCATTCACTTTGTGGGTGTTCAGGTGCCGATATGGAAGAATTCAAGGAAATCTATATGTGGGTTCGCTAGAATTCAAGGTGTTCCTTATGAGAATGAATTTAGAGCGTGCAAAAGTCTTTTAGAGTCCTGCCGCCGGGAGTTGTTGTCTGATTCTATCTCGGAATTGGATTTTCTTTTGAACAGAGATTCACCACCGGATTCCGACATCAGAGGTCTGTCTCTAAATTATGCATCAAGCTGTTTCCTGTTTGCTCCTGTTGAAATTTTTTAGCTGTTTGCATTCATATTTCTTCTTGATTTTTCTCCCATTCTGTTAACCAGTTTCTATCTCATTGAAGAGCTTGATCCAGTGTTTCCTGAAAGTATTGCTTGAACTTTCATCTATTTCTCTCCTTCACTTTCTTCTTCCTTTTCTCGTTCTGTTAACATTGAGTAATGTTCTAATATTTTTCAACACAGATTTATGATCTGCTACCATTATTCATTCAGAGTTTTAGTCATTCCACCAGTTATTTTATTTTTGTTTTCATCTTTGGTTATGATATTGGCCATTGATTTTCTCCTGAGTGGATATCTCATAGGAGTAGACATTGAAGAACCATGTGAAGCTTGTGATGATGAGAAGCAAAGAGCTGCAATTGCTATTAGTAACATCTTATCTGTCCTAACTCATCACAGTGAGGTAACTGGCAGATTGGTGTGCGAAAGGAGATGCAGCGTGCCTAGGGTGGGCATTCTTTGTTCATCCTTGAATACATCTCTTAACAGAATTAAACAGAGCTTTGTATTGTGAGTTATTCCAGACTAGTACTTCTGTTTATGCCTAAATCTTGTGTTTTATAAGCGAATTACGTTATCCTGTTCACTTTTGGATTCATGTCATGTCTCTGCATTTTTGCAGAACTGATCCGAACTTACCAGACATGCCTATAGTTTATGCGAGTGACGAGTTTTTGAAATTAACAGGTGCTAAACTAAACATTGAGGAAACTATATAGCATTTAAGATTTTGATGCCTAAAGATTCTGATTGAACAATACATTACAATTTTTCTTGCCTTGCATGCCTTAGGTTATACTAGACGTGAAGTGCTTGGGCGCAACTGTAGATTCTTAAGTGGAATTGACACGGATTCTTCAACCCTTTTTAAGGTTGTGTATTTCCTCCTTTCTGGTTGAACAAGCATTTTTCACCCGAGTTTCTGATGAGTTATAGAAATAATTGTTATGAATCTGATTTTCTTTGCCAGATAAAGGAAAGTCTTCAATCTGAACAAGCTTGCACTGTACGGATTTTAAATTACAGGTTGATATTATCTACAACATCTTATACAATGCGTAATTTGTAGTCTTCAGAATAAAAGACAACTTTCTAACTGCTCTTACAACAGGAAAAATAAGAGCTCATTTTGGAATGATCTCCACATATCACCTGTTCGAAATGCTTCAGGAAAGGTATTGTTGCTTTCTTTAGTATTGATTGAAATTTTGGGATTCTCTAGTTCTAATAATGTCCACGTTTTCTGAATTGAGTTAAAAAAACATTGTTTGCCTTCTTTAGTCATAGTACATTGTGCACGATCACCCATCTAAGACAAGCACTGTATTTAGTTCAAATTCAGAGATTGTTTCGGAGCTGTTGTTGAATGTAACTTTCTATTTTTATTTTCAGAAATGTTTTTAAGTGCTGAATTTGGATGATTCATATAGATTAACGTTATTATAATGTTAGAGATTGCTATGTTATGTTTGAATGCTCGATTTACAAACCGCAACTAAGGAAGGAACAAAACTTAAAAAAAGAAAGAAAAAAACAAAAATACTTAGGGAAGGAACCTAAAGGTGGGTGGATTGTGAGATCCCACATCGGTTAGAGAGGCGAACAAAGCACTCTTTATAAAGGCTTGGATTGGAAATCTCTCCCTAGTAGACGCCTTTTAAAAACTTTAAGAGGAAGTTCGAAAGGAAAAACTCAAAGAAGACAATATCTGCTAGCGGTGGACATGAGCTTAGGCATGGACTGTTACAAGATAATCCCTGTAAAACAATTCAAAGAATAGTTATGAAACCTTGCTTTCATAATGTTTCTTTGCCCCAGTATTTGATCAATGTGTATAACAAGAAGTGTACTAATGCCCACTATTGACACCTTTTGACATTTTATAGCTGAATTTACCAAGAAAGCTTTTCTATTTCCTTACCAAGTGAATGGTATTGTTTGCAGGTAGCGTACTTTGTGGGTGTCCAGATGGATGAAGATGATAAAAAGCAGGATGAACATGGGTTGAACCCTAAGATGAAACAACTTAGCACCGTGGGTGCAGTCAGGGTGGCCGTGAGGAGCTTGTCCATGACCGTGGGATGTTCACGGGGCTAGCCCGTTCGTCTCGTTTTGAATAGTAATATTAAATTTTCACTGTAATTACATCTCCTGAAGAGGTATATAAGAAAGATAAGACAGTAGATGTTCTTAAACGCTATTGTCAAGTTCTCATTTTTCTAATATGGAATGTGAAGCGTCTCAATTTTAAGTTGAAATAGCGGGCATTCTTTTGATTTATGCGTTAGCGGCTGTGTTTACATTGTAGCTTATTAGTAAGGGTGAAGTTTAGATTGTTGTGTGGACTGATTAAAATTAAATATTATCATACCATTGGGAAGGATCG

mRNA sequence

AGCGTCGGTGCCCTCGTTTGCTTCTGTTCTCAGCCCATCAATACATTGTCCCTCATCTACGTGGACACTTCTTGTCACAATGATTGGGACTACCATCGGCACACCCTCTCTAATTCCTCCGTTTTTCGGTAAATCTGTGGCAACTCAGCCAGAAAATTATACCTTGATCTTCATGATTTACATTCTTGTTATCTTTTTGATATGATCAGAAGCTTAGAACAATGGGATAACCATTTTTCCTTGTTTTTTTTGTAAATATTTTGTGGCCAAAGGAGGGGGTAATATCAACCAGAAATATTGTGCCGCGAATTTGTGAACGGCGTTGGTTCTGCTTTCTTTTTCTTCATTGCTGGTTTGATATTTTTCATTTTCCAATCTCGACGGCGTGATGGGTGATTTGATTTCTGGATCATTTTCATTTCAATTTTCTCATCGCATCGAAATATGGATAGTTGCGAACAATTTTTGTTGATCACAATTTACGCGATATTTTTCATTCGGATGTTCTCTTATTCACAGTCATATGGACACTCGTAGTTGCTAAGTGTGTTCTCCCCGCGAGTAGCTTGTCGAATCCCTGAGTCGATACGGATTCATTCTAGGGTTTTTGTTTGTTGTTCTTCTTGATTTTATATACTGAAGTTCAAAGCTTCTTCGAGAGACTGAACCAGAGGTTTGAGTGCCTCATGGGATTGGGACTATTCCGAATATCGAGGAAGAAAGGGGTTTTAGTCCAACAAGTTGAAGAACCAGGAGGATGGAATTTCAATTGGGTTTGATTGAACAATCACTTAACAGTCGTTATTCGCTCTGGGTCCGTGAAGCTCTTAATGATTTGTCGGATAATTTTACAATCACTGATCCCAGTATGTCGGGTCACCCAATTGTGTTCGTGAGCCGTGGATTCTTGAAGATGACTGGATATACCAAAGAAGAGGTGATTGGAAAAAATGGGAGGATGTTTCAGGGCCCAAAAACTTGTAGGAGTTCAGTAATGCAAATTCGCGAGGCAATTCGGGAAGAGAAGGGGATTCAAATTAATTTGCTAAATTATCGTAAAGATGGTACACCCTTCTGGGTCTTTTTTCAACTGAGCCCCGTCTTCTGCAAGGAAGATGGGCGGATCATTCACTTTGTGGGTGTTCAGGTGCCGATATGGAAGAATTCAAGGAAATCTATATGTGGGTTCGCTAGAATTCAAGGTGTTCCTTATGAGAATGAATTTAGAGCGTGCAAAAGTCTTTTAGAGTCCTGCCGCCGGGAGTTGTTGTCTGATTCTATCTCGGAATTGGATTTTCTTTTGAACAGAGATTCACCACCGGATTCCGACATCAGAGGAGTAGACATTGAAGAACCATGTGAAGCTTGTGATGATGAGAAGCAAAGAGCTGCAATTGCTATTAGTAACATCTTATCTGTCCTAACTCATCACAGTGAGGTAACTGGCAGATTGGTGTGCGAAAGGAGATGCAGCGTGCCTAGGGTGGGCATTCTTTGTTCATCCTTGAATACATCTCTTAACAGAATTAAACAGAGCTTTGTATTAACTGATCCGAACTTACCAGACATGCCTATAGTTTATGCGAGTGACGAGTTTTTGAAATTAACAGGTTATACTAGACGTGAAGTGCTTGGGCGCAACTGTAGATTCTTAAGTGGAATTGACACGGATTCTTCAACCCTTTTTAAGATAAAGGAAAGTCTTCAATCTGAACAAGCTTGCACTGTACGGATTTTAAATTACAGGAAAAATAAGAGCTCATTTTGGAATGATCTCCACATATCACCTGTTCGAAATGCTTCAGGAAAGGTAGCGTACTTTGTGGGTGTCCAGATGGATGAAGATGATAAAAAGCAGGATGAACATGGGTTGAACCCTAAGATGAAACAACTTAGCACCGTGGGTGCAGTCAGGGTGGCCGTGAGGAGCTTGTCCATGACCGTGGGATGTTCACGGGGCTAGCCCGTTCGTCTCGTTTTGAATAGTAATATTAAATTTTCACTGTAATTACATCTCCTGAAGAGGTATATAAGAAAGATAAGACAGTAGATGTTCTTAAACGCTATTGTCAAGTTCTCATTTTTCTAATATGGAATGTGAAGCGTCTCAATTTTAAGTTGAAATAGCGGGCATTCTTTTGATTTATGCGTTAGCGGCTGTGTTTACATTGTAGCTTATTAGTAAGGGTGAAGTTTAGATTGTTGTGTGGACTGATTAAAATTAAATATTATCATACCATTGGGAAGGATCG

Coding sequence (CDS)

ATGGAATTTCAATTGGGTTTGATTGAACAATCACTTAACAGTCGTTATTCGCTCTGGGTCCGTGAAGCTCTTAATGATTTGTCGGATAATTTTACAATCACTGATCCCAGTATGTCGGGTCACCCAATTGTGTTCGTGAGCCGTGGATTCTTGAAGATGACTGGATATACCAAAGAAGAGGTGATTGGAAAAAATGGGAGGATGTTTCAGGGCCCAAAAACTTGTAGGAGTTCAGTAATGCAAATTCGCGAGGCAATTCGGGAAGAGAAGGGGATTCAAATTAATTTGCTAAATTATCGTAAAGATGGTACACCCTTCTGGGTCTTTTTTCAACTGAGCCCCGTCTTCTGCAAGGAAGATGGGCGGATCATTCACTTTGTGGGTGTTCAGGTGCCGATATGGAAGAATTCAAGGAAATCTATATGTGGGTTCGCTAGAATTCAAGGTGTTCCTTATGAGAATGAATTTAGAGCGTGCAAAAGTCTTTTAGAGTCCTGCCGCCGGGAGTTGTTGTCTGATTCTATCTCGGAATTGGATTTTCTTTTGAACAGAGATTCACCACCGGATTCCGACATCAGAGGAGTAGACATTGAAGAACCATGTGAAGCTTGTGATGATGAGAAGCAAAGAGCTGCAATTGCTATTAGTAACATCTTATCTGTCCTAACTCATCACAGTGAGGTAACTGGCAGATTGGTGTGCGAAAGGAGATGCAGCGTGCCTAGGGTGGGCATTCTTTGTTCATCCTTGAATACATCTCTTAACAGAATTAAACAGAGCTTTGTATTAACTGATCCGAACTTACCAGACATGCCTATAGTTTATGCGAGTGACGAGTTTTTGAAATTAACAGGTTATACTAGACGTGAAGTGCTTGGGCGCAACTGTAGATTCTTAAGTGGAATTGACACGGATTCTTCAACCCTTTTTAAGATAAAGGAAAGTCTTCAATCTGAACAAGCTTGCACTGTACGGATTTTAAATTACAGGAAAAATAAGAGCTCATTTTGGAATGATCTCCACATATCACCTGTTCGAAATGCTTCAGGAAAGGTAGCGTACTTTGTGGGTGTCCAGATGGATGAAGATGATAAAAAGCAGGATGAACATGGGTTGAACCCTAAGATGAAACAACTTAGCACCGTGGGTGCAGTCAGGGTGGCCGTGAGGAGCTTGTCCATGACCGTGGGATGTTCACGGGGCTAG

Protein sequence

MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKEDGRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDFLLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSVPRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLSGIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQMDEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG
Homology
BLAST of CmaCh04G016300 vs. ExPASy Swiss-Prot
Match: O64511 (Protein TWIN LOV 1 OS=Arabidopsis thaliana OX=3702 GN=TLP1 PE=1 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 3.0e-117
Identity = 222/394 (56.35%), Postives = 285/394 (72.34%), Query Frame = 0

Query: 9   EQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRM 68
           E S + RY+LW++EAL +L  NFTITDP +SGHPIVF S GFLKMTGY++EEVIG+NG++
Sbjct: 17  EDSFSGRYTLWIKEALEELPHNFTITDPFISGHPIVFASLGFLKMTGYSREEVIGRNGKV 76

Query: 69  FQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKEDGRIIHFVG 128
           FQGPKT R S+M+IREAIREE+ +Q++LLNYRK G+PFW+ F + PVF K+DG++ +FV 
Sbjct: 77  FQGPKTNRRSIMEIREAIREERSVQVSLLNYRKSGSPFWMLFHMCPVFGKDDGKVTNFVA 136

Query: 129 VQVPI--WKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDFLLNRDS 188
           VQVPI   ++ RK +     +                 SCRRE+   +    D  L  + 
Sbjct: 137 VQVPISGREHHRKKLRNVGDLSS--------DTSPTFGSCRREVCFGNFVCQDRALPVEC 196

Query: 189 PPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSVPRVGIL 248
             D D +G++  E CEA + EK +A  AI+N+LS+L H+SE++GRLVC +R  +  V  L
Sbjct: 197 --DDDEQGLEDWEQCEASESEKLKATEAINNVLSILVHYSELSGRLVCGKRYCLRGVDCL 256

Query: 249 CSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLSGIDTDS 308
            SSL  SL RIKQSFVLT+P LPDMPI+YASD FL LTGY R+EVLG+NCRFLSG+DTDS
Sbjct: 257 SSSLVISLGRIKQSFVLTNPCLPDMPIIYASDAFLTLTGYKRQEVLGQNCRFLSGVDTDS 316

Query: 309 STLFKIKESLQSEQACTVRILNY--RKNKSSFWNDLHISPVRNASGKVAYFVGVQMDEDD 368
           S L+++KE +   Q+CTV+ILNY  RK+KSSFWN LHISPVRNASGK AYFVGVQ++   
Sbjct: 317 SVLYEMKECILKGQSCTVQILNYSNRKDKSSFWNLLHISPVRNASGKTAYFVGVQVEASC 376

Query: 369 KKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGC 399
           +  +   L P+ +QLS VGAVRVAVRS S+ V C
Sbjct: 377 RNTEIKELRPETRQLSVVGAVRVAVRS-SLMVTC 399

BLAST of CmaCh04G016300 vs. ExPASy Swiss-Prot
Match: P93025 (Phototropin-2 OS=Arabidopsis thaliana OX=3702 GN=PHOT2 PE=1 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 9.6e-55
Identity = 129/380 (33.95%), Postives = 208/380 (54.74%), Query Frame = 0

Query: 15  RYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRMFQGPKT 74
           R S  ++ AL+ L   F ++D +    PIV+ S GF  MTGY+ +E++G+N R  QGP T
Sbjct: 119 RVSQELKTALSTLQQTFVVSDATQPHCPIVYASSGFFTMTGYSSKEIVGRNCRFLQGPDT 178

Query: 75  CRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED-GRIIHFVGVQVPI 134
            ++ V +IR+ ++  K     LLNY+KDGTPFW    ++P+  K+D G  I F+G+QV +
Sbjct: 179 DKNEVAKIRDCVKNGKSYCGRLLNYKKDGTPFWNLLTVTPI--KDDQGNTIKFIGMQVEV 238

Query: 135 WKNSRKSICGFARIQG-----VPYE--NEFRACKSLLESC-----RRELLSDSISELDFL 194
            K +        R  G     + Y+   + +A  S+ E       R+  + +S+S  D +
Sbjct: 239 SKYTEGVNDKALRPNGLSKSLIRYDARQKEKALDSITEVVQTIRHRKSQVQESVSN-DTM 298

Query: 195 LNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAI---SNILSVLTHHSEVTGRLVCERRC 254
           +  DS   +   G    +  EA    +    ++    S + S    H ++      E   
Sbjct: 299 VKPDS-STTPTPGRQTRQSDEASKSFRTPGRVSTPTGSKLKSSNNRHEDLLRMEPEELML 358

Query: 255 SVPRVGILCS--------------SLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLT 314
           S   +G   S               L T+L RI+++FV++DP LPD PI++ASD FL+LT
Sbjct: 359 STEVIGQRDSWDLSDRERDIRQGIDLATTLERIEKNFVISDPRLPDNPIIFASDSFLELT 418

Query: 315 GYTRREVLGRNCRFLSGIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISP 365
            Y+R E+LGRNCRFL G +TD +T+ KI+++++ ++  TV+++NY K+   FWN  H+ P
Sbjct: 419 EYSREEILGRNCRFLQGPETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLFHLQP 478

BLAST of CmaCh04G016300 vs. ExPASy Swiss-Prot
Match: Q2RBR1 (Phototropin-1B OS=Oryza sativa subsp. japonica OX=39947 GN=PHOT1B PE=1 SV=2)

HSP 1 Score: 211.5 bits (537), Expect = 1.8e-53
Identity = 133/406 (32.76%), Postives = 206/406 (50.74%), Query Frame = 0

Query: 15  RYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRMFQGPKT 74
           R S  +R AL+     F ++D +   HPI++ S GF  MTGYT +EV+G+N R  QG  T
Sbjct: 122 RVSEELRAALSVFQQTFVVSDATHPNHPIMYASAGFFNMTGYTSKEVVGRNCRFLQGSGT 181

Query: 75  CRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKEDGRIIHFVGVQVPIW 134
               + +IR+++         +LNY+KDGTPFW    ++P+   EDGR++ F+G+QV + 
Sbjct: 182 DPHEIDKIRQSLANGSNYCGRILNYKKDGTPFWNLLTIAPI-KDEDGRLLKFIGMQVEVS 241

Query: 135 KNSRKSICGFARIQG-----VPYENE---------------FRACKSLLESCR------- 194
           K +        R  G     + Y+                  +  +SL ES         
Sbjct: 242 KYTEGKKDTVVRPNGLSESLIKYDARQKDHARSSVSELLLALKNPRSLSESSNNTLKRKS 301

Query: 195 RELLSDSISELDFLLNRDSPPDSDIRG---VDIEEPCEACDDEKQRAAIAISNILSVL-T 254
           +E LS S+SE+    + +S    + R      +++  E  D   +     +   +  L  
Sbjct: 302 QESLSMSMSEVPSKRSSESGSRRNSRSGTRSSLQKINEVPDQVNRTRKSGLRAFMGFLGM 361

Query: 255 HHSEVTGRLVCER--------------------RCSVPRVGILCSSLNTSLNRIKQSFVL 314
            H  V   ++  R                    R    R GI    L T+L RI+++FV+
Sbjct: 362 GHGSVEKNMLKPRDEDPLIDSDDERPESFEDEFRRKEMRRGI---DLATTLERIEKNFVI 421

Query: 315 TDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLSGIDTDSSTLFKIKESLQSEQACT 370
           TDP LPD PI++ASD FL+LT Y R E+LGRNCRFL G +TD +T+ KI++++ ++   T
Sbjct: 422 TDPRLPDNPIIFASDSFLQLTEYNREEILGRNCRFLQGPETDRATVRKIRDAIDNQAEVT 481

BLAST of CmaCh04G016300 vs. ExPASy Swiss-Prot
Match: Q2QYY8 (Phototropin-1A OS=Oryza sativa subsp. japonica OX=39947 GN=PHOT1A PE=1 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 3.1e-53
Identity = 132/406 (32.51%), Postives = 206/406 (50.74%), Query Frame = 0

Query: 15  RYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRMFQGPKT 74
           R S  +R AL+     F ++D +   HPI++ S GF  MTGYT +EV+G+N R  QG  T
Sbjct: 122 RVSEELRAALSAFQQTFVVSDATHPNHPIMYASAGFFNMTGYTSKEVVGRNCRFLQGSGT 181

Query: 75  CRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKEDGRIIHFVGVQVPIW 134
               + +IR+++         +LNY+KDGTPFW    ++P+   EDGR++ F+G+QV + 
Sbjct: 182 DPHEIDKIRQSLANGSNYCGRILNYKKDGTPFWNLLTIAPI-KDEDGRLLKFIGMQVEVS 241

Query: 135 KNSRKSICGFARIQG-----VPYENE---------------FRACKSLLESCR------- 194
           K +        R  G     + Y+                  +  +SL ES         
Sbjct: 242 KYTEGKKDTVVRPNGLSESLIKYDARQKDHARSSVSELLLALKNPRSLSESSNNTLKRKS 301

Query: 195 RELLSDSISELDFLLNRDSPPDSDIRG---VDIEEPCEACDDEKQRAAIAISNILSVL-T 254
           +E LS S++E+    + +S    + R      +++  E  D   +     +   +  L  
Sbjct: 302 QESLSMSMTEVPSKRSSESGSRRNSRSGTRSSLQKINEVPDQGNRTRKSGLRAFMGFLGM 361

Query: 255 HHSEVTGRLVCER--------------------RCSVPRVGILCSSLNTSLNRIKQSFVL 314
            H  V   ++  R                    R    R GI    L T+L RI+++FV+
Sbjct: 362 GHGSVEKNMLKPRDEDPLIDSDDERPESFEDEFRRKEMRRGI---DLATTLERIEKNFVI 421

Query: 315 TDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLSGIDTDSSTLFKIKESLQSEQACT 370
           TDP LPD PI++ASD FL+LT Y R E+LGRNCRFL G +TD +T+ KI++++ ++   T
Sbjct: 422 TDPRLPDNPIIFASDSFLQLTEYNREEILGRNCRFLQGPETDRATVRKIRDAIDNQAEVT 481

BLAST of CmaCh04G016300 vs. ExPASy Swiss-Prot
Match: Q8LPD9 (Phototropin OS=Chlamydomonas reinhardtii OX=3055 GN=PHOT PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.5e-52
Identity = 128/344 (37.21%), Postives = 183/344 (53.20%), Query Frame = 0

Query: 22  EALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRMFQGPKTCRSSVMQ 81
           + L  L   F + D ++   P+V+ S GF  MTGY  +EV+G N R  QG  T    V +
Sbjct: 13  KVLAGLRHTFVVADATLPDCPLVYASEGFYAMTGYGPDEVLGHNCRFLQGEGTDPKEVQK 72

Query: 82  IREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKEDGRIIHFVGVQVPIWKNSRKSI 141
           IR+AI++ +   + LLNYRKDGTPFW    ++P+    DGR+  FVGVQV +   S+   
Sbjct: 73  IRDAIKKGEACSVRLLNYRKDGTPFWNLLTVTPI-KTPDGRVSKFVGVQVDV--TSKTEG 132

Query: 142 CGFARIQGVP----YENEFRACKSLLESCRRELLSDSISELDFLLNRDSPPDSDIRGVDI 201
              A   GVP    Y++  R      ++  R ++ D    ++              GV  
Sbjct: 133 KALADNSGVPLLVKYDHRLR------DNVARTIVDDVTIAVE-----------KAEGV-- 192

Query: 202 EEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSVPRVGILCSSLNTSLNRI 261
            EP +A       +A+A +  L                   S PRV +    L T++ RI
Sbjct: 193 -EPGQA-------SAVAAAAPLGAKGPRGTAPK--------SFPRVAL---DLATTVERI 252

Query: 262 KQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLSGIDTDSSTLFKIKESLQ 321
           +Q+F ++DP LPD PIV+ASD FL+LTGY+R EVLGRNCRFL G  TD  T+ +I+ +++
Sbjct: 253 QQNFCISDPTLPDCPIVFASDAFLELTGYSREEVLGRNCRFLQGAGTDRGTVDQIRAAIK 312

Query: 322 SEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQMD 362
                TVRILNY K   +FWN   ++P+R+  G   +FVGVQ+D
Sbjct: 313 EGSELTVRILNYTKAGKAFWNMFTLAPMRDQDGHARFFVGVQVD 315

BLAST of CmaCh04G016300 vs. ExPASy TrEMBL
Match: A0A6J1KI06 (protein TWIN LOV 1 OS=Cucurbita maxima OX=3661 GN=LOC111493936 PE=4 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.5e-231
Identity = 401/401 (100.00%), Postives = 401/401 (100.00%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE
Sbjct: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED
Sbjct: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF
Sbjct: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
           LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV
Sbjct: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
           PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS
Sbjct: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG
Sbjct: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 401

BLAST of CmaCh04G016300 vs. ExPASy TrEMBL
Match: A0A6J1GXM3 (protein TWIN LOV 1 OS=Cucurbita moschata OX=3662 GN=LOC111458420 PE=4 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.3e-227
Identity = 394/401 (98.25%), Postives = 398/401 (99.25%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           MEFQLGLIEQSLNSRYSLWVREALNDLSDNFT+TDPS+SGHPIVFVSRGFLKMTGYTKEE
Sbjct: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTLTDPSISGHPIVFVSRGFLKMTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED
Sbjct: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           GRIIHFVGVQVPIWKNSRKSICGF+R QGVPYE EFRACKSLLESCRRELLSDSISELDF
Sbjct: 121 GRIIHFVGVQVPIWKNSRKSICGFSRTQGVPYEKEFRACKSLLESCRRELLSDSISELDF 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
           LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV
Sbjct: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
           PRVGILCSSLNTSLNRIKQSFVLTD NLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS
Sbjct: 241 PRVGILCSSLNTSLNRIKQSFVLTDLNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCS+G
Sbjct: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSQG 401

BLAST of CmaCh04G016300 vs. ExPASy TrEMBL
Match: A0A5A7SYN9 (Protein TWIN LOV 1 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00240 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.0e-205
Identity = 361/401 (90.02%), Postives = 376/401 (93.77%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           ME  LGLIEQSLNSRYSLWVREALN+LSDNFTITDPS++GHPIVFVS GFLK TGYTKEE
Sbjct: 1   MELPLGLIEQSLNSRYSLWVREALNNLSDNFTITDPSIAGHPIVFVSPGFLKTTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNGRMFQGP+T RSSVM IREA+R+EK IQINLLNYRKDGTPFWVFFQ++PVF KED
Sbjct: 61  VIGKNGRMFQGPETSRSSVMLIREAVRQEKEIQINLLNYRKDGTPFWVFFQMTPVFSKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           GRIIHFVGVQVPI KNSRKS  GF RIQGV YENEFRACKS L SCRREL+SDSISELD 
Sbjct: 121 GRIIHFVGVQVPILKNSRKSRRGFLRIQGVSYENEFRACKSFLGSCRRELMSDSISELDC 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
            LNRD  PDSD RGV+IEEPCEACDDEKQRAAIAISNIL VLTHHSEVTG LVCERRCS+
Sbjct: 181 TLNRDPQPDSDSRGVEIEEPCEACDDEKQRAAIAISNILFVLTHHSEVTGGLVCERRCSL 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
            RVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTR EVLGRNCRFLS
Sbjct: 241 SRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRCEVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           DEDDKKQDEHG NPKM+QLSTVGAV+VAVRSLSMT+GCS+G
Sbjct: 361 DEDDKKQDEHGFNPKMRQLSTVGAVKVAVRSLSMTMGCSQG 401

BLAST of CmaCh04G016300 vs. ExPASy TrEMBL
Match: A0A0A0KSK8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G604290 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.0e-205
Identity = 359/401 (89.53%), Postives = 376/401 (93.77%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           ME  LGLIEQSLNSRYSLWVREALNDLSDNFTITDP ++GHPIVFVS GFLKMTGYTKEE
Sbjct: 1   MELPLGLIEQSLNSRYSLWVREALNDLSDNFTITDPCIAGHPIVFVSPGFLKMTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNG+MFQGP+T RSSVM IREA+REEK IQINLLNYRKDGTPFWVFFQ++PVF KED
Sbjct: 61  VIGKNGKMFQGPETSRSSVMLIREAVREEKEIQINLLNYRKDGTPFWVFFQMTPVFSKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           G+IIHFVGVQVPI KNSRKS CGF RIQG  +ENEFRACKS L SCRREL+SDSISELD 
Sbjct: 121 GQIIHFVGVQVPILKNSRKSRCGFVRIQGDSHENEFRACKSFLGSCRRELMSDSISELDC 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
            LNRDS PDS+ RGV+IEEPCEACDDEKQRAAIAISNIL VLTHHSEVTG LVCERRCS+
Sbjct: 181 TLNRDSQPDSNSRGVEIEEPCEACDDEKQRAAIAISNILFVLTHHSEVTGGLVCERRCSL 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
           PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTR EVLGRNCRFLS
Sbjct: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRCEVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLH+SPV NASGK+AYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHVSPVHNASGKLAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           D DDKKQDEHGLNPK KQLSTVGAV+VAVRSLSMTVGCS+G
Sbjct: 361 DADDKKQDEHGLNPKTKQLSTVGAVKVAVRSLSMTVGCSQG 401

BLAST of CmaCh04G016300 vs. ExPASy TrEMBL
Match: A0A1S3BFK1 (protein TWIN LOV 1 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103489085 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.0e-205
Identity = 361/401 (90.02%), Postives = 376/401 (93.77%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           ME  LGLIEQSLNSRYSLWVREALN+LSDNFTITDPS++GHPIVFVS GFLK TGYTKEE
Sbjct: 1   MELPLGLIEQSLNSRYSLWVREALNNLSDNFTITDPSIAGHPIVFVSPGFLKTTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNGRMFQGP+T RSSVM IREA+R+EK IQINLLNYRKDGTPFWVFFQ++PVF KED
Sbjct: 61  VIGKNGRMFQGPETSRSSVMLIREAVRQEKEIQINLLNYRKDGTPFWVFFQMTPVFSKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           GRIIHFVGVQVPI KNSRKS  GF RIQGV YENEFRACKS L SCRREL+SDSISELD 
Sbjct: 121 GRIIHFVGVQVPILKNSRKSRRGFLRIQGVSYENEFRACKSFLGSCRRELMSDSISELDC 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
            LNRD  PDSD RGV+IEEPCEACDDEKQRAAIAISNIL VLTHHSEVTG LVCERRCS+
Sbjct: 181 TLNRDPQPDSDSRGVEIEEPCEACDDEKQRAAIAISNILFVLTHHSEVTGGLVCERRCSL 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
            RVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTR EVLGRNCRFLS
Sbjct: 241 SRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRCEVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           DEDDKKQDEHG NPKM+QLSTVGAV+VAVRSLSMT+GCS+G
Sbjct: 361 DEDDKKQDEHGFNPKMRQLSTVGAVKVAVRSLSMTMGCSQG 401

BLAST of CmaCh04G016300 vs. NCBI nr
Match: XP_022999675.1 (protein TWIN LOV 1 [Cucurbita maxima] >XP_022999759.1 protein TWIN LOV 1 [Cucurbita maxima])

HSP 1 Score: 811.6 bits (2095), Expect = 3.0e-231
Identity = 401/401 (100.00%), Postives = 401/401 (100.00%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE
Sbjct: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED
Sbjct: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF
Sbjct: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
           LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV
Sbjct: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
           PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS
Sbjct: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG
Sbjct: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 401

BLAST of CmaCh04G016300 vs. NCBI nr
Match: KAG6601409.1 (Protein TWIN LOV 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 806.2 bits (2081), Expect = 1.3e-229
Identity = 398/401 (99.25%), Postives = 400/401 (99.75%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           MEFQL LIEQSLNSRYSLWVREALNDLSDNFTITDPS+SGHPIVFVSRGFLKMTGYTKEE
Sbjct: 1   MEFQLDLIEQSLNSRYSLWVREALNDLSDNFTITDPSISGHPIVFVSRGFLKMTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED
Sbjct: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           GRIIHFVGVQVPIWKNSRKSICGF+RIQGVPYENEFRACKSLLESCRRELLSDSISELDF
Sbjct: 121 GRIIHFVGVQVPIWKNSRKSICGFSRIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
           LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV
Sbjct: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
           PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS
Sbjct: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG
Sbjct: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 401

BLAST of CmaCh04G016300 vs. NCBI nr
Match: XP_023537915.1 (protein TWIN LOV 1 [Cucurbita pepo subsp. pepo] >XP_023537920.1 protein TWIN LOV 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 805.1 bits (2078), Expect = 2.8e-229
Identity = 398/401 (99.25%), Postives = 400/401 (99.75%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPS+SGHPIVFVSRGFLKMTGYTKEE
Sbjct: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSISGHPIVFVSRGFLKMTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED
Sbjct: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           GRIIHFVGVQVPIWKNSRKSICGF+RIQGVPYENEFRA KSLLESCRRELLSDSISELDF
Sbjct: 121 GRIIHFVGVQVPIWKNSRKSICGFSRIQGVPYENEFRASKSLLESCRRELLSDSISELDF 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
           LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV
Sbjct: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
           PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS
Sbjct: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG
Sbjct: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 401

BLAST of CmaCh04G016300 vs. NCBI nr
Match: KAG7032188.1 (Protein TWIN LOV 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 803.1 bits (2073), Expect = 1.1e-228
Identity = 397/401 (99.00%), Postives = 399/401 (99.50%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           MEFQL LIEQSLNSRYSLWVREALNDLSDNFTITDPS+SGHPIVFVSRGFLKMTGYTKEE
Sbjct: 1   MEFQLDLIEQSLNSRYSLWVREALNDLSDNFTITDPSISGHPIVFVSRGFLKMTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED
Sbjct: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           GRIIHFVGVQVPIWKNSRKSICGF+RIQGVPYENEFRACKSLLESCRRELLSDSISELDF
Sbjct: 121 GRIIHFVGVQVPIWKNSRKSICGFSRIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
           LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV
Sbjct: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
           PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS
Sbjct: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIK SLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKLSLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG
Sbjct: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 401

BLAST of CmaCh04G016300 vs. NCBI nr
Match: XP_022956846.1 (protein TWIN LOV 1 [Cucurbita moschata] >XP_022956847.1 protein TWIN LOV 1 [Cucurbita moschata])

HSP 1 Score: 798.5 bits (2061), Expect = 2.6e-227
Identity = 394/401 (98.25%), Postives = 398/401 (99.25%), Query Frame = 0

Query: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEE 60
           MEFQLGLIEQSLNSRYSLWVREALNDLSDNFT+TDPS+SGHPIVFVSRGFLKMTGYTKEE
Sbjct: 1   MEFQLGLIEQSLNSRYSLWVREALNDLSDNFTLTDPSISGHPIVFVSRGFLKMTGYTKEE 60

Query: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120
           VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED
Sbjct: 61  VIGKNGRMFQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED 120

Query: 121 GRIIHFVGVQVPIWKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDF 180
           GRIIHFVGVQVPIWKNSRKSICGF+R QGVPYE EFRACKSLLESCRRELLSDSISELDF
Sbjct: 121 GRIIHFVGVQVPIWKNSRKSICGFSRTQGVPYEKEFRACKSLLESCRRELLSDSISELDF 180

Query: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240
           LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV
Sbjct: 181 LLNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSV 240

Query: 241 PRVGILCSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300
           PRVGILCSSLNTSLNRIKQSFVLTD NLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS
Sbjct: 241 PRVGILCSSLNTSLNRIKQSFVLTDLNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLS 300

Query: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360
           GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM
Sbjct: 301 GIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQM 360

Query: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSRG 402
           DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCS+G
Sbjct: 361 DEDDKKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGCSQG 401

BLAST of CmaCh04G016300 vs. TAIR 10
Match: AT2G02710.2 (PAS/LOV protein B )

HSP 1 Score: 428.3 bits (1100), Expect = 6.7e-120
Identity = 222/392 (56.63%), Postives = 285/392 (72.70%), Query Frame = 0

Query: 9   EQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRM 68
           E S + RY+LW++EAL +L  NFTITDP +SGHPIVF S GFLKMTGY++EEVIG+NG++
Sbjct: 17  EDSFSGRYTLWIKEALEELPHNFTITDPFISGHPIVFASLGFLKMTGYSREEVIGRNGKV 76

Query: 69  FQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKEDGRIIHFVG 128
           FQGPKT R S+M+IREAIREE+ +Q++LLNYRK G+PFW+ F + PVF K+DG++ +FV 
Sbjct: 77  FQGPKTNRRSIMEIREAIREERSVQVSLLNYRKSGSPFWMLFHMCPVFGKDDGKVTNFVA 136

Query: 129 VQVPI--WKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDFLLNRDS 188
           VQVPI   ++ RK +     +                 SCRRE+   +    D  L  + 
Sbjct: 137 VQVPISGREHHRKKLRNVGDLSS--------DTSPTFGSCRREVCFGNFVCQDRALPVEC 196

Query: 189 PPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSVPRVGIL 248
             D D +G++  E CEA + EK +A  AI+N+LS+L H+SE++GRLVC +R  +  V  L
Sbjct: 197 --DDDEQGLEDWEQCEASESEKLKATEAINNVLSILVHYSELSGRLVCGKRYCLRGVDCL 256

Query: 249 CSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLSGIDTDS 308
            SSL  SL RIKQSFVLT+P LPDMPI+YASD FL LTGY R+EVLG+NCRFLSG+DTDS
Sbjct: 257 SSSLVISLGRIKQSFVLTNPCLPDMPIIYASDAFLTLTGYKRQEVLGQNCRFLSGVDTDS 316

Query: 309 STLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGKVAYFVGVQMDEDDKK 368
           S L+++KE +   Q+CTV+ILNYRK+KSSFWN LHISPVRNASGK AYFVGVQ++   + 
Sbjct: 317 SVLYEMKECILKGQSCTVQILNYRKDKSSFWNLLHISPVRNASGKTAYFVGVQVEASCRN 376

Query: 369 QDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGC 399
            +   L P+ +QLS VGAVRVAVRS S+ V C
Sbjct: 377 TEIKELRPETRQLSVVGAVRVAVRS-SLMVTC 397

BLAST of CmaCh04G016300 vs. TAIR 10
Match: AT2G02710.1 (PAS/LOV protein B )

HSP 1 Score: 423.3 bits (1087), Expect = 2.1e-118
Identity = 222/394 (56.35%), Postives = 285/394 (72.34%), Query Frame = 0

Query: 9   EQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRM 68
           E S + RY+LW++EAL +L  NFTITDP +SGHPIVF S GFLKMTGY++EEVIG+NG++
Sbjct: 17  EDSFSGRYTLWIKEALEELPHNFTITDPFISGHPIVFASLGFLKMTGYSREEVIGRNGKV 76

Query: 69  FQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKEDGRIIHFVG 128
           FQGPKT R S+M+IREAIREE+ +Q++LLNYRK G+PFW+ F + PVF K+DG++ +FV 
Sbjct: 77  FQGPKTNRRSIMEIREAIREERSVQVSLLNYRKSGSPFWMLFHMCPVFGKDDGKVTNFVA 136

Query: 129 VQVPI--WKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDFLLNRDS 188
           VQVPI   ++ RK +     +                 SCRRE+   +    D  L  + 
Sbjct: 137 VQVPISGREHHRKKLRNVGDLSS--------DTSPTFGSCRREVCFGNFVCQDRALPVEC 196

Query: 189 PPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSVPRVGIL 248
             D D +G++  E CEA + EK +A  AI+N+LS+L H+SE++GRLVC +R  +  V  L
Sbjct: 197 --DDDEQGLEDWEQCEASESEKLKATEAINNVLSILVHYSELSGRLVCGKRYCLRGVDCL 256

Query: 249 CSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLSGIDTDS 308
            SSL  SL RIKQSFVLT+P LPDMPI+YASD FL LTGY R+EVLG+NCRFLSG+DTDS
Sbjct: 257 SSSLVISLGRIKQSFVLTNPCLPDMPIIYASDAFLTLTGYKRQEVLGQNCRFLSGVDTDS 316

Query: 309 STLFKIKESLQSEQACTVRILNY--RKNKSSFWNDLHISPVRNASGKVAYFVGVQMDEDD 368
           S L+++KE +   Q+CTV+ILNY  RK+KSSFWN LHISPVRNASGK AYFVGVQ++   
Sbjct: 317 SVLYEMKECILKGQSCTVQILNYSNRKDKSSFWNLLHISPVRNASGKTAYFVGVQVEASC 376

Query: 369 KKQDEHGLNPKMKQLSTVGAVRVAVRSLSMTVGC 399
           +  +   L P+ +QLS VGAVRVAVRS S+ V C
Sbjct: 377 RNTEIKELRPETRQLSVVGAVRVAVRS-SLMVTC 399

BLAST of CmaCh04G016300 vs. TAIR 10
Match: AT2G02710.3 (PAS/LOV protein B )

HSP 1 Score: 388.7 bits (997), Expect = 5.9e-108
Identity = 197/345 (57.10%), Postives = 253/345 (73.33%), Query Frame = 0

Query: 9   EQSLNSRYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRM 68
           E S + RY+LW++EAL +L  NFTITDP +SGHPIVF S GFLKMTGY++EEVIG+NG++
Sbjct: 17  EDSFSGRYTLWIKEALEELPHNFTITDPFISGHPIVFASLGFLKMTGYSREEVIGRNGKV 76

Query: 69  FQGPKTCRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKEDGRIIHFVG 128
           FQGPKT R S+M+IREAIREE+ +Q++LLNYRK G+PFW+ F + PVF K+DG++ +FV 
Sbjct: 77  FQGPKTNRRSIMEIREAIREERSVQVSLLNYRKSGSPFWMLFHMCPVFGKDDGKVTNFVA 136

Query: 129 VQVPI--WKNSRKSICGFARIQGVPYENEFRACKSLLESCRRELLSDSISELDFLLNRDS 188
           VQVPI   ++ RK +     +                 SCRRE+   +    D  L  + 
Sbjct: 137 VQVPISGREHHRKKLRNVGDLSS--------DTSPTFGSCRREVCFGNFVCQDRALPVEC 196

Query: 189 PPDSDIRGVDIEEPCEACDDEKQRAAIAISNILSVLTHHSEVTGRLVCERRCSVPRVGIL 248
             D D +G++  E CEA + EK +A  AI+N+LS+L H+SE++GRLVC +R  +  V  L
Sbjct: 197 --DDDEQGLEDWEQCEASESEKLKATEAINNVLSILVHYSELSGRLVCGKRYCLRGVDCL 256

Query: 249 CSSLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLTGYTRREVLGRNCRFLSGIDTDS 308
            SSL  SL RIKQSFVLT+P LPDMPI+YASD FL LTGY R+EVLG+NCRFLSG+DTDS
Sbjct: 257 SSSLVISLGRIKQSFVLTNPCLPDMPIIYASDAFLTLTGYKRQEVLGQNCRFLSGVDTDS 316

Query: 309 STLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISPVRNASGK 352
           S L+++KE +   Q+CTV+ILNYRK+KSSFWN LHISPVRNASGK
Sbjct: 317 SVLYEMKECILKGQSCTVQILNYRKDKSSFWNLLHISPVRNASGK 351

BLAST of CmaCh04G016300 vs. TAIR 10
Match: AT5G58140.4 (phototropin 2 )

HSP 1 Score: 215.7 bits (548), Expect = 6.8e-56
Identity = 129/380 (33.95%), Postives = 208/380 (54.74%), Query Frame = 0

Query: 15  RYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRMFQGPKT 74
           R S  ++ AL+ L   F ++D +    PIV+ S GF  MTGY+ +E++G+N R  QGP T
Sbjct: 119 RVSQELKTALSTLQQTFVVSDATQPHCPIVYASSGFFTMTGYSSKEIVGRNCRFLQGPDT 178

Query: 75  CRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED-GRIIHFVGVQVPI 134
            ++ V +IR+ ++  K     LLNY+KDGTPFW    ++P+  K+D G  I F+G+QV +
Sbjct: 179 DKNEVAKIRDCVKNGKSYCGRLLNYKKDGTPFWNLLTVTPI--KDDQGNTIKFIGMQVEV 238

Query: 135 WKNSRKSICGFARIQG-----VPYE--NEFRACKSLLESC-----RRELLSDSISELDFL 194
            K +        R  G     + Y+   + +A  S+ E       R+  + +S+S  D +
Sbjct: 239 SKYTEGVNDKALRPNGLSKSLIRYDARQKEKALDSITEVVQTIRHRKSQVQESVSN-DTM 298

Query: 195 LNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAI---SNILSVLTHHSEVTGRLVCERRC 254
           +  DS   +   G    +  EA    +    ++    S + S    H ++      E   
Sbjct: 299 VKPDS-STTPTPGRQTRQSDEASKSFRTPGRVSTPTGSKLKSSNNRHEDLLRMEPEELML 358

Query: 255 SVPRVGILCS--------------SLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLT 314
           S   +G   S               L T+L RI+++FV++DP LPD PI++ASD FL+LT
Sbjct: 359 STEVIGQRDSWDLSDRERDIRQGIDLATTLERIEKNFVISDPRLPDNPIIFASDSFLELT 418

Query: 315 GYTRREVLGRNCRFLSGIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISP 365
            Y+R E+LGRNCRFL G +TD +T+ KI+++++ ++  TV+++NY K+   FWN  H+ P
Sbjct: 419 EYSREEILGRNCRFLQGPETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLFHLQP 478

BLAST of CmaCh04G016300 vs. TAIR 10
Match: AT5G58140.2 (phototropin 2 )

HSP 1 Score: 215.7 bits (548), Expect = 6.8e-56
Identity = 129/380 (33.95%), Postives = 208/380 (54.74%), Query Frame = 0

Query: 15  RYSLWVREALNDLSDNFTITDPSMSGHPIVFVSRGFLKMTGYTKEEVIGKNGRMFQGPKT 74
           R S  ++ AL+ L   F ++D +    PIV+ S GF  MTGY+ +E++G+N R  QGP T
Sbjct: 119 RVSQELKTALSTLQQTFVVSDATQPHCPIVYASSGFFTMTGYSSKEIVGRNCRFLQGPDT 178

Query: 75  CRSSVMQIREAIREEKGIQINLLNYRKDGTPFWVFFQLSPVFCKED-GRIIHFVGVQVPI 134
            ++ V +IR+ ++  K     LLNY+KDGTPFW    ++P+  K+D G  I F+G+QV +
Sbjct: 179 DKNEVAKIRDCVKNGKSYCGRLLNYKKDGTPFWNLLTVTPI--KDDQGNTIKFIGMQVEV 238

Query: 135 WKNSRKSICGFARIQG-----VPYE--NEFRACKSLLESC-----RRELLSDSISELDFL 194
            K +        R  G     + Y+   + +A  S+ E       R+  + +S+S  D +
Sbjct: 239 SKYTEGVNDKALRPNGLSKSLIRYDARQKEKALDSITEVVQTIRHRKSQVQESVSN-DTM 298

Query: 195 LNRDSPPDSDIRGVDIEEPCEACDDEKQRAAIAI---SNILSVLTHHSEVTGRLVCERRC 254
           +  DS   +   G    +  EA    +    ++    S + S    H ++      E   
Sbjct: 299 VKPDS-STTPTPGRQTRQSDEASKSFRTPGRVSTPTGSKLKSSNNRHEDLLRMEPEELML 358

Query: 255 SVPRVGILCS--------------SLNTSLNRIKQSFVLTDPNLPDMPIVYASDEFLKLT 314
           S   +G   S               L T+L RI+++FV++DP LPD PI++ASD FL+LT
Sbjct: 359 STEVIGQRDSWDLSDRERDIRQGIDLATTLERIEKNFVISDPRLPDNPIIFASDSFLELT 418

Query: 315 GYTRREVLGRNCRFLSGIDTDSSTLFKIKESLQSEQACTVRILNYRKNKSSFWNDLHISP 365
            Y+R E+LGRNCRFL G +TD +T+ KI+++++ ++  TV+++NY K+   FWN  H+ P
Sbjct: 419 EYSREEILGRNCRFLQGPETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLFHLQP 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O645113.0e-11756.35Protein TWIN LOV 1 OS=Arabidopsis thaliana OX=3702 GN=TLP1 PE=1 SV=2[more]
P930259.6e-5533.95Phototropin-2 OS=Arabidopsis thaliana OX=3702 GN=PHOT2 PE=1 SV=2[more]
Q2RBR11.8e-5332.76Phototropin-1B OS=Oryza sativa subsp. japonica OX=39947 GN=PHOT1B PE=1 SV=2[more]
Q2QYY83.1e-5332.51Phototropin-1A OS=Oryza sativa subsp. japonica OX=39947 GN=PHOT1A PE=1 SV=2[more]
Q8LPD91.5e-5237.21Phototropin OS=Chlamydomonas reinhardtii OX=3055 GN=PHOT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KI061.5e-231100.00protein TWIN LOV 1 OS=Cucurbita maxima OX=3661 GN=LOC111493936 PE=4 SV=1[more]
A0A6J1GXM31.3e-22798.25protein TWIN LOV 1 OS=Cucurbita moschata OX=3662 GN=LOC111458420 PE=4 SV=1[more]
A0A5A7SYN94.0e-20590.02Protein TWIN LOV 1 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A0A0KSK84.0e-20589.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G604290 PE=4 SV=1[more]
A0A1S3BFK14.0e-20590.02protein TWIN LOV 1 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103489085 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022999675.13.0e-231100.00protein TWIN LOV 1 [Cucurbita maxima] >XP_022999759.1 protein TWIN LOV 1 [Cucurb... [more]
KAG6601409.11.3e-22999.25Protein TWIN LOV 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023537915.12.8e-22999.25protein TWIN LOV 1 [Cucurbita pepo subsp. pepo] >XP_023537920.1 protein TWIN LOV... [more]
KAG7032188.11.1e-22899.00Protein TWIN LOV 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022956846.12.6e-22798.25protein TWIN LOV 1 [Cucurbita moschata] >XP_022956847.1 protein TWIN LOV 1 [Cucu... [more]
Match NameE-valueIdentityDescription
AT2G02710.26.7e-12056.63PAS/LOV protein B [more]
AT2G02710.12.1e-11856.35PAS/LOV protein B [more]
AT2G02710.35.9e-10857.10PAS/LOV protein B [more]
AT5G58140.46.8e-5633.95phototropin 2 [more]
AT5G58140.26.8e-5633.95phototropin 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001610PAC motifSMARTSM00086pac_2coord: 323..365
e-value: 1.2E-6
score: 38.1
coord: 93..136
e-value: 0.0055
score: 25.9
IPR000014PAS domainSMARTSM00091pas_2coord: 248..321
e-value: 0.057
score: 22.6
coord: 18..87
e-value: 1.4
score: 17.8
IPR000014PAS domainPFAMPF13426PAS_9coord: 40..131
e-value: 3.5E-17
score: 62.6
coord: 260..361
e-value: 2.7E-18
score: 66.1
IPR000014PAS domainTIGRFAMTIGR00229TIGR00229coord: 273..369
e-value: 2.3E-11
score: 41.9
coord: 43..128
e-value: 9.5E-11
score: 39.9
IPR000014PAS domainPROSITEPS50112PAScoord: 273..295
score: 10.167795
IPR000014PAS domainPROSITEPS50112PAScoord: 20..89
score: 11.519805
IPR000014PAS domainCDDcd00130PAScoord: 43..133
e-value: 1.56016E-7
score: 47.2427
IPR000014PAS domainCDDcd00130PAScoord: 269..361
e-value: 1.69485E-8
score: 49.9391
NoneNo IPR availableGENE3D3.30.450.20PAS domaincoord: 30..170
e-value: 1.3E-37
score: 131.0
coord: 260..397
e-value: 1.5E-45
score: 156.8
NoneNo IPR availablePANTHERPTHR47429:SF2PROTEIN TWIN LOV 1coord: 8..393
NoneNo IPR availablePANTHERPTHR47429PROTEIN TWIN LOV 1coord: 8..393
IPR000700PAS-associated, C-terminalPROSITEPS50113PACcoord: 320..374
score: 12.022626
IPR035965PAS domain superfamilySUPERFAMILY55785PYP-like sensor domain (PAS domain)coord: 261..361
IPR035965PAS domain superfamilySUPERFAMILY55785PYP-like sensor domain (PAS domain)coord: 29..130

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G016300.1CmaCh04G016300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018298 protein-chromophore linkage
biological_process GO:0009637 response to blue light
cellular_component GO:0005634 nucleus
molecular_function GO:0009881 photoreceptor activity