CmaCh04G014550 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G014550
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	Cma_Chr04: 7405310 .. 7407529 (+)
RNA-Seq Expression	CmaCh04G014550
Synteny	CmaCh04G014550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATTTTGTCGGCGTTTCTTTTCCCTCCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCTGGGATTTCCGAGATTAACGGTAAGCATTTGCAGTACTCCAAGTTCTGGAATGCGAATAGTTCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTTCATTTCTTCAAGAGGATCTTCGAGGTACTCTCAATTTCTCTACGCCTCTGATTGAGTTCTGAAATTTCAATCCGATTTTAAAATTAATTCTTCGATTCAGTTCGATTGTGGCTTTATTGAGAACCTGTTAATCTTATTCTTCGAAAATTTCTCGAGTCCTTTATATAATTGTATGAAGATTCGTGGCGAAAAGAAAATGGCGGAGAGATCTGGATCACTTCTCCCTTCTTTGCATCTTATGGTTCCTCTGGAGATTTCGGAACTCGTCTTTTCGTTTACGGGATTAGGAAGACTTTTAACCTATCTTGATTTTTATTTATTTTTTTATTTTTTTGTCGTATGAACTTGAATAATTAACCATCCACTTATAAATATCCGACTTCATTAGTTTTCCTCTACTAATTTAATTGTCATTAAGAACTCGTGGAGTCTACTTTGTGTATAATAATAATTTTGGAATTATTATTATATGAAGTCATGTACAAGATCTAGAACAAACTGCACGTTTCTGCTCAACAGTTTTCTAAGAAATTTGCTTCAATAATCACTATAGATGATTTGCAATTATTGTACCTTTCATTCATGCGACAATTATTTTTAAAACAAATTGATTATTCTTCTCAAATTATAGAAGCTATTCCTGTATTATTTCTCCAACCAATTGCATCTGAGGGTAACTAGAAATGATAACAAAAGTTTTGGAAGATCATATGAATCATTTAGAGAACAATGAAAAGTAATAGAAACAAACATTTTTTCAAAAGCAACAAAACACCTAATCTTATATTAAATATCATTGAATTCAAAAGCTTAAATGGATAGACAATATGAATTACTTACTATCATGAAATTCTTTGCAGGTTCTGTTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTTGTCCACAGTGATCATGATATACACACAGCACCTATCACAAGGAATTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCACTTTCTCTTGTCCAAAACCCGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCTTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGGTACGTCTCCAAATTTGAAAACTTCCCCAGCCATGTCAAAGCCCTGCTCCCTTTTGTGTTTTAGAACCCTTCCTTTCTTCATTTCATAAAAGCTACTTGTGTTTATGCTTTACACAGTTAAGTTAGGTTCATACTACTATTGTTTTAGGCCTACCCTTAGGTTGTGATTCTATTTATCACTACAATAGTAGAAGGTAAAACAGTTGGATATTTGTTCTTTACCTGATCTTAATAATGACGATTTATTCCTGGTGTGTTTTTATTTATTGAAATTAATATAGTATTTATGTTATATAATTGAATTGAATTGAATTAAATTAAAAATGTTCTTGAAGCCTTAATCTGGAAAATTAGTCTAAAACTCCTAGGTTATATATTTATTTCTATGGACTTTGTGGACCAAATTGGTTGTTCACATTTGATAATAATATTGATTATCCTCCACCGTTGTTTGATAGCATTTGGACTTTAGTACAGAAGAATTAGGAGTATTTAAAAAGATTCGATAACTTGAAAGACTCATCAACCCGGGAATTATAATTATGAAATGAATTATAATTATGGAATGGCCACACGCTAAGAAAGAAAAGGACATGAAGGAGATTTTATTTATTTGTTTATTATTTTTTTTTTTTATAAATTGCAGA

mRNA sequence

ATGGGTATTTTGTCGGCGTTTCTTTTCCCTCCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCTGGGATTTCCGAGATTAACGGTAAGCATTTGCAGTACTCCAAGTTCTGGAATGCGAATAGTTCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTTCATTTCTTCAAGAGGATCTTCGAGGTTCTGTTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTTGTCCACAGTGATCATGATATACACACAGCACCTATCACAAGGAATTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCACTTTCTCTTGTCCAAAACCCGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCTTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGA

Coding sequence (CDS)

ATGGGTATTTTGTCGGCGTTTCTTTTCCCTCCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCTGGGATTTCCGAGATTAACGGTAAGCATTTGCAGTACTCCAAGTTCTGGAATGCGAATAGTTCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTTCATTTCTTCAAGAGGATCTTCGAGGTTCTGTTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTTGTCCACAGTGATCATGATATACACACAGCACCTATCACAAGGAATTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCACTTTCTCTTGTCCAAAACCCGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCTTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGA

Protein sequence

MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEPKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKMVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYATRK

Homology

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BLAST of CmaCh04G014550 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.0e-14
Identity = 46/149 (30.87%), Postives = 81/149 (54.36%), Query Frame = 0


Query: 86  FPSDNERILVLKAAL---TLHFFKRIFEVLFIHKYS--SKMVFDTAIKISFSYCLSTVIM 145
           + +D+ +    K AL   +LH+ KRI+E +F+H++S  +  +F+     S+ +  + ++ 
Sbjct: 116 YGTDSPKSFAQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVS 175

Query: 146 IYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSL 205
            +  H      E PI+   +G+ L++IG + N+  H  L   R  G T  KIP+G LF  
Sbjct: 176 YFVNH--PLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLRPAGSTERKIPRGLLFEF 235

Query: 206 VICPHYLFEIIEFFGFAFISQELYPLCFS 230
           V CP+Y  EI+ + GF+ ++Q L    F+
Sbjct: 236 VSCPNYTVEILSWIGFSILTQTLTSWIFA 262

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BLAST of CmaCh04G014550 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 3.0e-11
Identity = 35/80 (43.75%), Postives = 49/80 (61.25%), Query Frame = 0


Query: 161 GIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFIS 220
           G AL+L+G++ N +   +L   RKPGETGYKIP+GGLF  V   +Y  E++E+ GFA  S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213

Query: 221 QELYPLCFSTGTAIYLAGRS 241
             L  + F+  T   L  R+
Sbjct: 214 WSLQGVVFALFTLSTLLTRA 233

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BLAST of CmaCh04G014550 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.0e-11
Identity = 34/81 (41.98%), Postives = 50/81 (61.73%), Query Frame = 0


Query: 160 IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 219
           IG  L+L G++ N +   +L   RKPG+TGYKIP+GGLF  V   +Y  EI+E+ G+A  
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 220 SQELYPLCFSTGTAIYLAGRS 241
           S  +    F+  T  +L+GR+
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRA 237

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BLAST of CmaCh04G014550 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.8e-11
Identity = 46/148 (31.08%), Postives = 73/148 (49.32%), Query Frame = 0


Query: 95  VLKAALTLHFFKRIFEVLFIHKYSSKM-VFDTAIKISFSYCLSTVIMIYTQHLSQGIPEP 154
           +L A   +H+  R     F+ +    M +    + I F  C   +   Y  H +    + 
Sbjct: 86  ILLAMFLVHYGHRCLIYPFLMRGGKPMPLLACTMAIMFCTCNGYLQSRYLSHCAVYADDW 145

Query: 155 PIDLK-NIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIE 214
             D +  IG  L+L G++ N +   +L   RKPG+TGYKIP+GGLF  V   +Y  EI+E
Sbjct: 146 VTDPRFLIGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIME 205

Query: 215 FFGFAFISQELYPLCFSTGTAIYLAGRS 241
           + G+A  S  +    F+  T  +L+GR+
Sbjct: 206 WCGYALASWSVQGAAFAFFTFCFLSGRA 233

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BLAST of CmaCh04G014550 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 8.8e-11
Identity = 62/223 (27.80%), Postives = 99/223 (44.39%), Query Frame = 0


Query: 28  MGMAGISEINGKHLQYSKFWNANSSPKAPS----EEPKLSSKYGMLLAYTPAFLAGAASF 87
           +   G+  +   + +YS  W+    P  P+    E P ++  + +     PA    AA  
Sbjct: 22  VAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMA--WPLYECIRPA----AARL 81

Query: 88  WFFPSDNERILVLKAALTLHFFKR--IFEVLFIHKYSSKMVFDTAIKISF----SYCLST 147
              P+      VL A   +H+ +R  +F VL I      ++F   +   F     Y  S 
Sbjct: 82  GNLPN-----RVLLAMFLIHYVQRTLVFPVL-IRGGKPTLLFTFVLAFLFCTLNGYLQSR 141

Query: 148 VIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGL 207
            +  +  +    +  P       G AL+L+G++ N +   +L   RKPGETGYKIP+GGL
Sbjct: 142 YLSQFAVYAEDWVTHPCF---LTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGL 201

Query: 208 FSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 241
           F  V   +Y  E++E+ GFA  S  L  + F+  T   L  R+
Sbjct: 202 FEYVSSANYFGELVEWCGFALASWSLQGVVFALFTLCALFTRA 229

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BLAST of CmaCh04G014550 vs. ExPASy TrEMBL
Match: A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 2.7e-135
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRK 246
           YATRK
Sbjct: 241 YATRK 245

[Close]

BLAST of CmaCh04G014550 vs. ExPASy TrEMBL
Match: A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 4.5e-135
Identity = 244/245 (99.59%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVFDTAIKISFSYCLSTVIMIYTQHLSQG+PEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRK 246
           YATRK
Sbjct: 241 YATRK 245

[Close]

BLAST of CmaCh04G014550 vs. ExPASy TrEMBL
Match: A0A1S3BFS0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.1e-112
Identity = 206/245 (84.08%), Postives = 220/245 (89.80%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M ILSAFLFPPPPSTFVNVMTVVSST++ MAG+SEI GKHLQYSKFWNANS  K  ++E 
Sbjct: 11  MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLL YTPAFLAGAASFW FPS +ER  +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 71  KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 130

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV  TAI+ISFSY LST IMIYTQHLSQG+PEPPIDLKNIG+ LFL GIIGNFYHH+LLS
Sbjct: 131 MVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHYLLS 190

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           +TRK  ET YKIPKGGLFS VICPHYLFEII +FGFAFISQELYPLCFS GTAIYLAGRS
Sbjct: 191 QTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 250

Query: 241 YATRK 246
           YATRK
Sbjct: 251 YATRK 255

[Close]

BLAST of CmaCh04G014550 vs. ExPASy TrEMBL
Match: A0A6J1DAI6 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111018692 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.3e-109
Identity = 199/245 (81.22%), Postives = 217/245 (88.57%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M I SAFLFPPPPSTFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE 
Sbjct: 1   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFEVLF+HKYSSK
Sbjct: 61  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 120

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV DTA KIS SY LST IMIYTQHLSQG P P IDLKNIGI  F++GIIGNFYHH+LLS
Sbjct: 121 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 180

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           +TR  GE  YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+TGTA+YLAGRS
Sbjct: 181 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 240

Query: 241 YATRK 246
           YATRK
Sbjct: 241 YATRK 245

[Close]

BLAST of CmaCh04G014550 vs. ExPASy TrEMBL
Match: A0A0A0KTC7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612880 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.5e-101
Identity = 188/245 (76.73%), Postives = 206/245 (84.08%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN + K  +EE 
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFW FPS +ER                  VLFIHKYSSK
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDER------------------VLFIHKYSSK 140

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV  TAI+IS SY +ST IMIYTQHLSQG+PEPPIDLKNIG+  FLIGIIGNFYHH+LLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           +TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 247

Query: 241 YATRK 246
           YATRK
Sbjct: 261 YATRK 247

[Close]

BLAST of CmaCh04G014550 vs. NCBI nr
Match: XP_022984951.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])

HSP 1 Score: 491.1 bits (1263), Expect = 5.5e-135
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRK 246
           YATRK
Sbjct: 241 YATRK 245

[Close]

BLAST of CmaCh04G014550 vs. NCBI nr
Match: XP_022957108.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 490.3 bits (1261), Expect = 9.4e-135
Identity = 244/245 (99.59%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVFDTAIKISFSYCLSTVIMIYTQHLSQG+PEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRK 246
           YATRK
Sbjct: 241 YATRK 245

[Close]

BLAST of CmaCh04G014550 vs. NCBI nr
Match: XP_023528760.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 484.6 bits (1246), Expect = 5.1e-133
Identity = 241/245 (98.37%), Postives = 243/245 (99.18%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEI GKHLQYSKFWNANS+PKAPSEEP
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEIKGKHLQYSKFWNANSAPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVFDTAIKISFSYC STVIMIYTQHLSQG+PEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 121 MVFDTAIKISFSYCSSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRK 246
           YATRK
Sbjct: 241 YATRK 245

[Close]

BLAST of CmaCh04G014550 vs. NCBI nr
Match: KAG7032032.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 484.2 bits (1245), Expect = 6.7e-133
Identity = 242/245 (98.78%), Postives = 243/245 (99.18%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           MGILSAFLFPPP STFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEE 
Sbjct: 300 MGILSAFLFPPPASTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEET 359

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK
Sbjct: 360 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 419

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MVFDTAIKISFSYCLSTVIMIYTQHLSQG+PEPPIDLKNIGIALFLIGIIGNFYHHFLLS
Sbjct: 420 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 479

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS
Sbjct: 480 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 539

Query: 241 YATRK 246
           YATRK
Sbjct: 540 YATRK 544

[Close]

BLAST of CmaCh04G014550 vs. NCBI nr
Match: XP_038891781.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 427.6 bits (1098), Expect = 7.5e-116
Identity = 213/245 (86.94%), Postives = 227/245 (92.65%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M +LSAFLFPPPPS FVN+MTVVSSTA+ MAGISEINGKHLQYSKFWNAN++PK  SE  
Sbjct: 34  MDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSEVS 93

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
            LSSKYGMLLAYTPAFLAGAASFW FPS +ERIL+LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 94  MLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSSK 153

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV  TAI+ISFSY LSTVIMIYTQHLSQG+PEPPI+LKNIGI LFLIGIIGNFYHH+LLS
Sbjct: 154 MVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLS 213

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           KTRK GET Y+IPKGGLFS VICPHYLFEIIEFFGFAFISQELYPLCFS GT+IYLAGRS
Sbjct: 214 KTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRS 273

Query: 241 YATRK 246
           YATRK
Sbjct: 274 YATRK 278

[Close]

BLAST of CmaCh04G014550 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 274.2 bits (700), Expect = 9.8e-74
Identity = 137/246 (55.69%), Postives = 181/246 (73.58%), Query Frame = 0


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEE- 60
           M ++++F++PPPPS  +N M+VV   A+   G SEI G HL+YSKF  ++SSP+   E  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 61  PKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSS 120
             +SS+ GMLL YTPAFLA A+SF+  PSD+ R L+LK+AL LHFFKR+FEVLFIHKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 121 KMVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLL 180
            M  D+A+ IS SY  ST +M+Y+Q+L+ G+ EP  D+K  G+ +F++GI+GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 181 SKTRK-PGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAG 240
           +K RK  G+  YKIPKGGLF ++ICPHYLFEI+ F+ F  ISQ +Y   F+ GT +YL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 241 RSYATR 245
           RSYATR
Sbjct: 241 RSYATR 246

[Close]

BLAST of CmaCh04G014550 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.7e-12
Identity = 44/138 (31.88%), Postives = 69/138 (50.00%), Query Frame = 0


Query: 103 HFFKRIFEVLFIHKYS--SKMVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLK-N 162
           H+FKRI E  F+H++S  +  + +     ++ +     I  Y  H    +  P  DL+  
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNH---PLYTPVSDLQMK 200

Query: 163 IGIALFLIGIIGNFYHHFLLSKTRKP-GETGYKIPKGGLFSLVICPHYLFEIIEFFGFAF 222
           IG    L+  + NFY H LL   R P G  GY+IP+G LF++V C +Y  EI ++ GF  
Sbjct: 201 IGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNI 260

Query: 223 ISQELYPLCFSTGTAIYL 237
            +Q +    F    A+ +
Sbjct: 261 ATQTIAGYVFLAVAALIM 275

[Close]

BLAST of CmaCh04G014550 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 52.8 bits (125), Expect = 4.7e-07
Identity = 30/86 (34.88%), Postives = 45/86 (52.33%), Query Frame = 0


Query: 160 IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 219
           IG+ +F+ G+  N      L + +K    GY IP+GG F LV CP+Y  E IE+ G+A +
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 220 SQELYPLCFSTGTAIYLAGRSYATRK 246
           +     + F   T   L  R+ A+ K
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHK 240

The following BLAST results are available for this feature:

BLAST of CmaCh04G014550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q55C17	1.0e-14	30.87	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]
P24008	3.0e-11	43.75	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]
Q28891	4.0e-11	41.98	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
P18405	8.8e-11	31.08	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
Q68FF9	8.8e-11	27.80	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]

BLAST of CmaCh04G014550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J6R3	2.7e-135	100.00	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1GZA3	4.5e-135	99.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A1S3BFS0	1.1e-112	84.08	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A6J1DAI6	3.3e-109	81.22	very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A0A0KTC7	2.5e-101	76.73	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128...	[more]

BLAST of CmaCh04G014550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022984951.1	5.5e-135	100.00	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima]	[more]
XP_022957108.1	9.4e-135	99.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]
XP_023528760.1	5.1e-133	98.37	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7032032.1	6.7e-133	98.78	3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_038891781.1	7.5e-116	86.94	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida]	[more]

BLAST of CmaCh04G014550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	9.8e-74	55.69	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	1.7e-12	31.88	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	4.7e-07	34.88	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 88..243 e-value: 2.0E-5 score: 26.4
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 6..245
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 156..240 e-value: 7.9E-20 score: 71.4
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 161..228 score: 25.787687
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 6..245

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G014550.1	CmaCh04G014550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors