CmaCh04G014540 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G014540
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	Cma_Chr04: 7400718 .. 7402853 (+)
RNA-Seq Expression	CmaCh04G014540
Synteny	CmaCh04G014540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTAAAGAAACTTTTACTCAGAGGCTTCGTTGATGGCGGCGATGGATACTATTTCGACGCTTCTTTTCCGGCCGCCGCCTTCACTGTTAGTGAACGTGATGACGGTGGTGAGTTCAGTGGTGGTGGCGGTGATCGGACTTTCGGAAGCGAAAGGAACGGCGCATTTACAGTATTCGAAATTCTGGAATACAAATAATAGCGCTACGAAATCATCTAGTCCGGAGAAATTCAAGATTTCATCAAGATCGGGGATGCTCTTACTTTATGCGCCGGCGGCTCTCGCCGGCGCTGTATCGTTCTGGATCTTTCCAAACGAAGATCTGAGGATTCTTCTGCTTAAATCTGCTCTCACTCTCCATTTCGTCAAAAGGAACTTTGAGGTACTCAAATTTTTTGCACTAAATTCCGTCTTCTTTTACATTCATCGCCATTCCTCAAATTCCGCCTGAAATTCCTACACAGGTACTGTTTATTCACAAATACAGCGGCAAAATGGCGCTCGATTCAGCCTTAATAATCTCCCTCAGCTACTTCTCCTCTACAGCGCTGATGATTTTCGCTCAAGAACTCTCAAGAACACTTCCAGAGCCATCCATTGATTTGAAATTCATCGTGTGAATGTTTGATTAATCCACCATACCTAAATGGTGTGAAAGTTATCTTATTTATAATCAAATGAATTACAAGGATATGGAAATAGTAATAAATGGAAAGATACCTATGATAATAAGGCAAATGTCTAATATTTGCCTTATAATAAAATATCAAATATAATATATATGGTAAACTATAATTTATTACTAAATATCCTTAACACCCCACCGCAAGCTAAGCGTCGGATTTAAACGAACGTGAAGCTTGAATCTGAAAAATTCAAAGAGGTTGAGAAACACTTTTCATGAAGATGCTAACAACCCGGTTCTTGGCCATCAAAAATTGCAGAGGGATCGCCGCTCAGCGGCGATGCTGCCTTGGCTTCAGCGAGAATATGTCTAACCCATGAAGAGGGATTGTCGCTTAGCGACGATGCTGCCTTGGCTCTGGTGAGAATATTTCTAACCCCAAGAAGTAGAAGGGGAAACCTAGAGCAAGGGACAAATACAATGTTGAAGCCGGAAGAGTCGCCACATTGGGCGAAGGAGCCAATTTTGGCGAGAAGGGCAGTCGGAAGAGTCGCCTCATCGGGCAAAGAAGCCAATTTTGGCAGGAAGGGCGGCGGCAACTTGGTTGGCGAATAGGCCAGTGCGGCACCCAGATTCCGAAGGTAAATCTCTGCAAAAGGTTGATAGGTTTAGGTTAGAGTTTATGTGAAGGATGAAGGTTGATTGAGAAGAAAATGAGAGAGACTATATTGGCCAAGGGGAAAACCGATTTGGTTGGAAAAGGTGAGGTTATATGTACAGAGTATGACGAAAGGTGATTTGGAGGTCAATAGGGTGGAGCCGAAGAGTAATCAATCGGCTCCGACTACGGCAGCCATGAAGACTACCCCTGCGGCGTCAAAACCAGCAGGGGTGGTGGAGAAGAAGATTAGAGGATTCAAAAGCCATTGAAAAGAAAGGATGGGGGTTGTTAAACCGAGAGGGCTCTGATACCATGTGAATGTTTGATTAATCCACCATACCTAAATGGTGTGAAAGTTATCTTATTTATAATCAAATGAATTACAAGGATATGGAAATAGTAATAAATGGAAATAGTAATAAATGGAAAGATACCTATGATAATAAGGCAAATGTCTAATATTTGCCTTATAATAAAATATCAAATATAATATATATGGTAAACTATAATTTATTACTAAATATCCTTAACGGAATTGCTCTGTTTCTGATAGGAATTATCGGGAACTTTTACCACCATTATATATTGTCCCAAACTAGAAAAAAGGGCGAAACAGGCTATAAAATCCCCAAAGGAGGCCTCTTCTGCGTTGTGATTTGCCCTCACTATCTGTTTGAAATAACTGTTTATTTCGGATTCGCGCTCATCTCTCAGACAATCTACTCATTGTGCTTCGCCATCGCCACGGCTATGTATTTGGGTGGGCGAAGCTACGCCACTAGGAAATGGTATGTCTCTAAATTTGAGGACTTCCCTCACCATATCAAAGCCCTTGTTCCCTTTGTATTTTAA

mRNA sequence

TGTAAAGAAACTTTTACTCAGAGGCTTCGTTGATGGCGGCGATGGATACTATTTCGACGCTTCTTTTCCGGCCGCCGCCTTCACTGTTAGTGAACGTGATGACGGTGGTGAGTTCAGTGGTGGTGGCGGTGATCGGACTTTCGGAAGCGAAAGGAACGGCGCATTTACAGTATTCGAAATTCTGGAATACAAATAATAGCGCTACGAAATCATCTAGTCCGGAGAAATTCAAGATTTCATCAAGATCGGGGATGCTCTTACTTTATGCGCCGGCGGCTCTCGCCGGCGCTGTATCGTTCTGGATCTTTCCAAACGAAGATCTGAGGATTCTTCTGCTTAAATCTGCTCTCACTCTCCATTTCGTCAAAAGGAACTTTGAGGGCGAAACAGGCTATAAAATCCCCAAAGGAGGCCTCTTCTGCGTTGTGATTTGCCCTCACTATCTGTTTGAAATAACTGTTTATTTCGGATTCGCGCTCATCTCTCAGACAATCTACTCATTGTGCTTCGCCATCGCCACGGCTATGTATTTGGGTGGGCGAAGCTACGCCACTAGGAAATGGTATGTCTCTAAATTTGAGGACTTCCCTCACCATATCAAAGCCCTTGTTCCCTTTGTATTTTAA

Coding sequence (CDS)

ATGGCGGCGATGGATACTATTTCGACGCTTCTTTTCCGGCCGCCGCCTTCACTGTTAGTGAACGTGATGACGGTGGTGAGTTCAGTGGTGGTGGCGGTGATCGGACTTTCGGAAGCGAAAGGAACGGCGCATTTACAGTATTCGAAATTCTGGAATACAAATAATAGCGCTACGAAATCATCTAGTCCGGAGAAATTCAAGATTTCATCAAGATCGGGGATGCTCTTACTTTATGCGCCGGCGGCTCTCGCCGGCGCTGTATCGTTCTGGATCTTTCCAAACGAAGATCTGAGGATTCTTCTGCTTAAATCTGCTCTCACTCTCCATTTCGTCAAAAGGAACTTTGAGGGCGAAACAGGCTATAAAATCCCCAAAGGAGGCCTCTTCTGCGTTGTGATTTGCCCTCACTATCTGTTTGAAATAACTGTTTATTTCGGATTCGCGCTCATCTCTCAGACAATCTACTCATTGTGCTTCGCCATCGCCACGGCTATGTATTTGGGTGGGCGAAGCTACGCCACTAGGAAATGGTATGTCTCTAAATTTGAGGACTTCCCTCACCATATCAAAGCCCTTGTTCCCTTTGTATTTTAA

Protein sequence

MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKSSSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFEGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLGGRSYATRKWYVSKFEDFPHHIKALVPFVF

Homology

[Close]

BLAST of CmaCh04G014540 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-13
Identity = 36/81 (44.44%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 117 GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLGGRSYATRK 176
           G+TGYKIP+GGLF  V   +Y  EI  + G+AL S ++    FA  T  +L GR+    +
Sbjct: 183 GDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHHR 242

Query: 177 WYVSKFEDFPHHIKALVPFVF 198
           WY+ KFE++P   K L+PF+F
Sbjct: 243 WYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of CmaCh04G014540 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.8e-13
Identity = 35/81 (43.21%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 117 GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLGGRSYATRK 176
           G+TGYKIP+GGLF  V   +Y  EI  + G+AL S ++    FA  T  +L GR+    +
Sbjct: 179 GDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHHE 238

Query: 177 WYVSKFEDFPHHIKALVPFVF 198
           WY+ KFE++P   K ++PF+F
Sbjct: 239 WYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of CmaCh04G014540 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.7e-12
Identity = 36/83 (43.37%), Postives = 56/83 (67.47%), Query Frame = 0


Query: 116 EGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLGGRSYATR 175
           +G+ GYKIP+GGLF +V CP+Y  EI  +FG+A+++ +     F + T   L  R+ ATR
Sbjct: 176 QGDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATR 235

Query: 176 KWYVSKF-EDFPHHIKALVPFVF 198
            WY+ KF +D+P   KA++PF++
Sbjct: 236 LWYLEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of CmaCh04G014540 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 6.4e-12
Identity = 36/80 (45.00%), Postives = 50/80 (62.50%), Query Frame = 0


Query: 117 GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLGGRSYATRK 176
           GETGYKIP+GGLF  V   +Y  E+  + GFAL S ++  + FA+ T   L  R+    +
Sbjct: 175 GETGYKIPRGGLFEYVSSANYFGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQ 234

Query: 177 WYVSKFEDFPHHIKALVPFV 197
           WY+ KFED+P   K L+PF+
Sbjct: 235 WYLEKFEDYPKTRKILIPFL 254

[Close]

BLAST of CmaCh04G014540 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 6.4e-12
Identity = 37/80 (46.25%), Postives = 49/80 (61.25%), Query Frame = 0


Query: 117 GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLGGRSYATRK 176
           GETGYKIP+GGLF  V   +Y  E+  + GFAL S ++  + FA+ T   L  R+    +
Sbjct: 179 GETGYKIPRGGLFEYVSAANYFGELVEWCGFALASWSLQGVVFALFTLSTLLTRAKQHHQ 238

Query: 177 WYVSKFEDFPHHIKALVPFV 197
           WY  KFED+P   K L+PFV
Sbjct: 239 WYHEKFEDYPKSRKILIPFV 258

[Close]

BLAST of CmaCh04G014540 vs. ExPASy TrEMBL
Match: A0A6J1J2Y2 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482885 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.1e-94
Identity = 197/272 (72.43%), Postives = 197/272 (72.43%), Query Frame = 0


Query: 1   MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS 60
           MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS
Sbjct: 1   MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS 60

Query: 61  SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE---- 120
           SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE    
Sbjct: 61  SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFEVLFI 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 HKYSGKMALDSALIISLSYFSSTALMIFAQELSRTLPEPSIDLKFIGIALFLIGIIGNFY 180

Query: 181 -----------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 198
                      GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM
Sbjct: 181 HHYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 240

[Close]

BLAST of CmaCh04G014540 vs. ExPASy TrEMBL
Match: A0A6J1H074 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458413 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.5e-92
Identity = 192/272 (70.59%), Postives = 195/272 (71.69%), Query Frame = 0


Query: 1   MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS 60
           MAAMDTIS LLFRPPPSLLVNVMTVVSSVVVAVIGLSEA+GTAHLQYSKFWNTNNSATKS
Sbjct: 1   MAAMDTISALLFRPPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNTNNSATKS 60

Query: 61  SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE---- 120
           S+ EKFKISSRSGMLLLYAPAALAGAVSFWIFPN+DLRILLLKSALTLHFVKRNFE    
Sbjct: 61  SNLEKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFVKRNFEVLFI 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 HKYSGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGIALFLIGIIGNFY 180

Query: 181 -----------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 198
                      GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM
Sbjct: 181 HHYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 240

[Close]

BLAST of CmaCh04G014540 vs. ExPASy TrEMBL
Match: A0A0A0KRA7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612890 PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.5e-72
Identity = 158/271 (58.30%), Postives = 174/271 (64.21%), Query Frame = 0


Query: 2   AAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKSS 61
           AAM+T+S   F  PPSLLVNVMTVVSSVVVAVIG SEA+GT HLQYSKFWNT+NSATKSS
Sbjct: 5   AAMNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGT-HLQYSKFWNTSNSATKSS 64

Query: 62  SPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE----- 121
           + E+ +ISSR GMLLLY PA LAGA SFW  PN+DLR+LLLKSALT HF KR+FE     
Sbjct: 65  NSEQLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVH 124

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 125 KYSGKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYH 184

Query: 182 ----------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMY 198
                     GETGYKIPKGGLF ++ICPHY+FEITVYFGFALISQT Y+  FAIATA+Y
Sbjct: 185 HILLSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIY 244

[Close]

BLAST of CmaCh04G014540 vs. ExPASy TrEMBL
Match: A0A1S3BGE8 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC103489361 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 7.5e-72
Identity = 159/269 (59.11%), Postives = 172/269 (63.94%), Query Frame = 0


Query: 4   MDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKSSSP 63
           M+ +S   F  PPSLLVNVMTVVSS VVAVIGLSEA+GT HLQYSKFWNT+NSATKSS+ 
Sbjct: 1   MNALSVFFFPMPPSLLVNVMTVVSSAVVAVIGLSEARGT-HLQYSKFWNTSNSATKSSNS 60

Query: 64  EKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE------- 123
           E+ +ISSR GMLLLY PAALAGA SFW FPN+D R+LLLKSALTLHF KR+FE       
Sbjct: 61  EQLRISSRLGMLLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKY 120

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 121 SGKMVLDSASIISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIALFLIGIIGNMYHHI 180

Query: 184 --------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLG 198
                   GETGYKIPKGGLF +VICPHYLFEITVYFGFALISQT YS  FAIATA+YLG
Sbjct: 181 LLSQTRKKGETGYKIPKGGLFGIVICPHYLFEITVYFGFALISQTFYSFFFAIATAIYLG 240

[Close]

BLAST of CmaCh04G014540 vs. ExPASy TrEMBL
Match: A0A5D3CBG2 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001750 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.4e-70
Identity = 156/258 (60.47%), Postives = 167/258 (64.73%), Query Frame = 0


Query: 15  PPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKSSSPEKFKISSRSGM 74
           PPSLLVNVMTVVSS VVAVIGLSEA+GT HLQYSKFWNT+NSATKSS+ E+ +ISSR GM
Sbjct: 2   PPSLLVNVMTVVSSAVVAVIGLSEARGT-HLQYSKFWNTSNSATKSSNSEQLRISSRLGM 61

Query: 75  LLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE------------------ 134
           LLLY PAALAGA SFW FPN+D R+LLLKSALTLHF KR+FE                  
Sbjct: 62  LLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKYSGKMVLDSASI 121

Query: 135 ---------------------------------------------------------GET 194
                                                                    GET
Sbjct: 122 ISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIALFLIGIIGNMYHHILLSQTRKKGET 181

Query: 195 GYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLGGRSYATRKWYV 198
           GYKIPKGGLF +VICPHYLFEITVYFGFALISQT YS  FAIATA+YLGGRSYAT KWYV
Sbjct: 182 GYKIPKGGLFGIVICPHYLFEITVYFGFALISQTFYSFFFAIATAIYLGGRSYATWKWYV 241

[Close]

BLAST of CmaCh04G014540 vs. NCBI nr
Match: XP_022984712.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita maxima])

HSP 1 Score: 355.9 bits (912), Expect = 2.2e-94
Identity = 197/272 (72.43%), Postives = 197/272 (72.43%), Query Frame = 0


Query: 1   MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS 60
           MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS
Sbjct: 1   MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS 60

Query: 61  SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE---- 120
           SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE    
Sbjct: 61  SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFEVLFI 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 HKYSGKMALDSALIISLSYFSSTALMIFAQELSRTLPEPSIDLKFIGIALFLIGIIGNFY 180

Query: 181 -----------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 198
                      GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM
Sbjct: 181 HHYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 240

[Close]

BLAST of CmaCh04G014540 vs. NCBI nr
Match: XP_022956834.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 346.7 bits (888), Expect = 1.3e-91
Identity = 192/272 (70.59%), Postives = 195/272 (71.69%), Query Frame = 0


Query: 1   MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS 60
           MAAMDTIS LLFRPPPSLLVNVMTVVSSVVVAVIGLSEA+GTAHLQYSKFWNTNNSATKS
Sbjct: 1   MAAMDTISALLFRPPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNTNNSATKS 60

Query: 61  SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE---- 120
           S+ EKFKISSRSGMLLLYAPAALAGAVSFWIFPN+DLRILLLKSALTLHFVKRNFE    
Sbjct: 61  SNLEKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFVKRNFEVLFI 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 HKYSGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGIALFLIGIIGNFY 180

Query: 181 -----------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 198
                      GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM
Sbjct: 181 HHYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 240

[Close]

BLAST of CmaCh04G014540 vs. NCBI nr
Match: KAG6601242.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 344.4 bits (882), Expect = 6.7e-91
Identity = 190/272 (69.85%), Postives = 194/272 (71.32%), Query Frame = 0


Query: 1   MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS 60
           MAAMDTIS LLFRPPPSLLVNVMTVVSSVVVAVIGLSEA+GTAHLQYSKFWN NNSATKS
Sbjct: 1   MAAMDTISALLFRPPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNANNSATKS 60

Query: 61  SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE---- 120
           S+PEKFKISSRSGMLLLYAPAALAGAVSFWIFPN+DLRILLLKSALTLHFVKRNFE    
Sbjct: 61  SNPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFVKRNFEVLFI 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 HKYSGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGISLFLIGIIGNFY 180

Query: 181 -----------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 198
                      GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM
Sbjct: 181 HHYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 240

[Close]

BLAST of CmaCh04G014540 vs. NCBI nr
Match: KAG7032032.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 340.9 bits (873), Expect = 7.4e-90
Identity = 188/270 (69.63%), Postives = 192/270 (71.11%), Query Frame = 0


Query: 1   MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS 60
           MAAMDTIS LLFRPPPSLLVNVMTVVSSVVVAVIGLSEA+GTAHLQYSKFWN NNSATKS
Sbjct: 1   MAAMDTISALLFRPPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNANNSATKS 60

Query: 61  SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE---- 120
           S+PEKFKISSRSGMLLLYAPAALAGAVSFWIFPN+DLRILLLKSALTLHFVKRNFE    
Sbjct: 61  SNPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFVKRNFEVLFI 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 HKYSGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGISLFLIGIIGNFY 180

Query: 181 -----------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 196
                      GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM
Sbjct: 181 HHYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 240

[Close]

BLAST of CmaCh04G014540 vs. NCBI nr
Match: XP_023518338.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 338.6 bits (867), Expect = 3.7e-89
Identity = 188/272 (69.12%), Postives = 192/272 (70.59%), Query Frame = 0


Query: 1   MAAMDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKS 60
           MAAMDTIS LLFR PPSLLVNVMTVVSSVVVAVIGLSEA+GTAHLQYSKFWN NNSATKS
Sbjct: 1   MAAMDTISALLFRLPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNANNSATKS 60

Query: 61  SSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE---- 120
           S+PEKFKISSRSGMLLLYAPAALAGAVSFWIFPN+DLRILLLKSALTLHF KRNFE    
Sbjct: 61  SNPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFFKRNFEVLFI 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 HKYSGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGISLFLIGIIGNFY 180

Query: 181 -----------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 198
                      GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM
Sbjct: 181 HHYILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAM 240

[Close]

BLAST of CmaCh04G014540 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 192.2 bits (487), Expect = 4.0e-49
Identity = 120/275 (43.64%), Postives = 148/275 (53.82%), Query Frame = 0


Query: 4   MDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKSSSP 63
           M+ +++ ++ PPPS+L+N M+VV    +A IG SE +G  HL+YSKF         SSSP
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGN-HLKYSKF------GVSSSSP 60

Query: 64  EKFK-----ISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFE-- 123
           +  K     ISSR+GMLLLY PA LA A SF++ P++DLR LLLKSAL LHF KR FE  
Sbjct: 61  QPQKERFGSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVL 120

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 121 FIHKYSGGMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGN 180

Query: 184 --------------GETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIA 198
                         G+  YKIPKGGLF ++ICPHYLFEI V++ F LISQTIYS  FA+ 
Sbjct: 181 LYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMG 240

[Close]

BLAST of CmaCh04G014540 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.2e-10
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 0


Query: 111 VKRNFEGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLGGR 170
           V+   E   GY IP+GG F +V CP+Y  E   + G+A+++ +   + F + T   L  R
Sbjct: 175 VRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPR 234

Query: 171 SYATRKWYVSKF-EDFPHHIKALVPFVF 198
           + A+ KWY++KF E++P   KA++PFV+
Sbjct: 235 ARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of CmaCh04G014540 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-08
Identity = 45/148 (30.41%), Postives = 73/148 (49.32%), Query Frame = 0


Query: 55  NSATKSSSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRN 114
           N  + +S+PE   +SS S ++ L +   + GA+  W + ++     +L S      ++ N
Sbjct: 205 NGKSHTSAPEFNLLSSISPLMKLGSLQWIGGAIFLWGWIHQRRCHAILGS------LREN 264

Query: 115 FEGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQ----TIYSLC-FAIATAMYLGG 174
                 Y IP G  F +V  PH+L EI +Y G  + S     TI+ L  F  A   Y  G
Sbjct: 265 PSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGFVAANLTYAAG 324

Query: 175 RSYATRKWYVSKFEDFPHHIKALVPFVF 198
               T +WY+ KFE++P +  A+ P+V+
Sbjct: 325 E---THRWYLRKFENYPANRHAIFPYVY 343

[Close]

BLAST of CmaCh04G014540 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-08
Identity = 45/148 (30.41%), Postives = 73/148 (49.32%), Query Frame = 0


Query: 55  NSATKSSSPEKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRN 114
           N  + +S+PE   +SS S ++ L +   + GA+  W + ++     +L S      ++ N
Sbjct: 204 NGKSHTSAPEFNLLSSISPLMKLGSLQWIGGAIFLWGWIHQRRCHAILGS------LREN 263

Query: 115 FEGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQ----TIYSLC-FAIATAMYLGG 174
                 Y IP G  F +V  PH+L EI +Y G  + S     TI+ L  F  A   Y  G
Sbjct: 264 PSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGFVAANLTYAAG 323

Query: 175 RSYATRKWYVSKFEDFPHHIKALVPFVF 198
               T +WY+ KFE++P +  A+ P+V+
Sbjct: 324 E---THRWYLRKFENYPANRHAIFPYVY 342

[Close]

BLAST of CmaCh04G014540 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 49.7 bits (117), Expect = 3.2e-06
Identity = 31/82 (37.80%), Postives = 43/82 (52.44%), Query Frame = 0


Query: 121 YKIPKGGLFCVVICPHYLFEITVYFGFALISQ----TIYSLC-FAIATAMYLGGRSYATR 180
           Y IP G  F +V CPH+L EI +Y G  + S     +I+ L  F  A   Y  G    T 
Sbjct: 242 YIIPYGDWFEMVSCPHFLAEIVLYLGLLISSGGTDISIWLLFGFVAANLTYAAGE---TH 301

Query: 181 KWYVSKFEDFPHHIKALVPFVF 198
           +WY+ KFE++P    A+ P V+
Sbjct: 302 RWYLQKFENYPASRHAIFPHVY 320

The following BLAST results are available for this feature:

BLAST of CmaCh04G014540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q28891	2.0e-13	44.44	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
P18405	5.8e-13	43.21	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
Q2QDF6	1.7e-12	43.37	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
Q68FF9	6.4e-12	45.00	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]
P24008	6.4e-12	46.25	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]

BLAST of CmaCh04G014540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J2Y2	1.1e-94	72.43	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1H074	6.5e-92	70.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A0A0KRA7	1.5e-72	58.30	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128...	[more]
A0A1S3BGE8	7.5e-72	59.11	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A5D3CBG2	1.4e-70	60.47	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194...	[more]

BLAST of CmaCh04G014540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022984712.1	2.2e-94	72.43	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita maxima]	[more]
XP_022956834.1	1.3e-91	70.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]
KAG6601242.1	6.7e-91	69.85	3-oxo-5-alpha-steroid 4-dehydrogenase 2, partial [Cucurbita argyrosperma subsp. ...	[more]
KAG7032032.1	7.4e-90	69.63	3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_023518338.1	3.7e-89	69.12	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh04G014540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	4.0e-49	43.64	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	1.2e-10	37.50	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	1.5e-08	30.41	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.2	1.5e-08	30.41	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	3.2e-06	37.80	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 111..197 e-value: 2.0E-22 score: 79.8
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 116..172 score: 18.320173
None	No IPR available	PANTHER	PTHR10556:SF52	BNAC02G06360D PROTEIN	coord: 116..197
None	No IPR available	PANTHER	PTHR10556:SF52	BNAC02G06360D PROTEIN	coord: 3..116
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 116..197 coord: 3..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G014540.1	CmaCh04G014540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors