CmaCh04G013900 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh04G013900
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionMajor facilitator superfamily protein
LocationCma_Chr04: 7104108 .. 7106001 (+)
RNA-Seq ExpressionCmaCh04G013900
SyntenyCmaCh04G013900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATCACCACAAAAATGGACCCAATCTCTGCAGGGTTCAACACCAAATCCTTCACCCTCAAACTAATCAAAGGGCCATGGTTCATGATGTTCGCGTCCTTCCTTATTATGTCCATGGCCGGCATACCTTACATGTTCGGCCTCTACTCCGGCACCATCAAATCTGTATTGGGTTACGACCAAACCACCCTTAATTTCATCAGCTTCTTCAAAGATGTCGGCACCACCGTCGGTGTCTTGGCCGGCTTAATCAACGAAGTAACCCCACCATGGTCCATTCTTGCCATGGGAGCTCTTCTCAATTTCTTCGGTTACTTCATGATTTGGCTCTCTGTTTCCGAGAAGATCCCCACCCATGTCTGGCTCATGTGCTTATATATCTGTGTCGGAGCCAACGCTACAACGTTTGCTAACACCGGCGCCCTCGTCACCTGCGTTAAGAATTACCCGCAACGGCGCGGCGTCGTCATCGGAATTCTTAAGGGCTACATGGGGTTGAGTGGGGCCATTGTTACGCAGCTCTACCATGCGATTTATGGGAAAGATGAAAAGTCGTTGATTTTGTTGTTGGGTTGGTTGCCGGCGGTGATTTCGTTTATTTTCTTGCCGACGGTGCGGCGGATGAAGGTGGAGCACGAGGAGGATGAGCTGAGGGTTTTTTACAGGTTTTTGTATATCTCGTTGGGGCTGGCCGGGTTTTTGATGTTGATGATAATTTTGCAGCAGAAATTTAGTTTTGGGAGAGGGGAGTACGGTGGCAGTGCGGCGGTGGTTACATTTTTGCTCCTTTTGCCGATTGCGGTTGTTGTTGCTCAGGAATTCAAGGCGTGGCGGCGCTTGAATCAGCCTCCGGTGGTTGAGAATGGGCTTTCCTACACGCCGGTGAGTGAAAATTTAATCAATTTTTTTTGGAAATTTTAAGGTTAGACAAAGTTGCCATTTTGATCAAACGTTAAAAACAGGGGACAGCGGCGTTGAAGAACACGACACCAATGGCACTCCTCCCGAAGAAACCAAAGGAGACAGAACCAGCTAGCAGCACCAAAATTCAATGGTGGAAAAACGTGTTCAATCCGCCAGAAAGAGGAGAAGATTGGACAATTCTCCAAGCTCTGTTCAGCTTCGACATGTTCCTCCTCTTTCTCGCCACCGCGTGCGGCGTCGGCGGGACACTAACCGCCATCGACAACCTCGGACAAATCGGACAGTCACAAGATTACCCAAAGAAAAGCATAAGCACATTCGTGTCGCTTGTCAGCATATGGAATTACCTCGGCAGAGTAATGGCCGGATTCCTCTCCGAACATTTGCTAATCAAATACAGATTCCCTCGTCCTTCAATGCTCACAATCGTTCTTCTTCTTTCCTGCATCGCCCATCTTCTGATCGCCTTCAACCCGAGCGGCGGCCTCTACATTGCCTCTGTTTTAACCGGATTCTGCTACGGCGCCCAGTGGCCTCTGCTTTTCGCCATCGTCTCAGAGGTTTTTGGGCTAAAATATTACGCGACGCTGTATAATTTCGGGTCAGTGGCGAGCCCTGTGGGGCTTTATTTGCTGAATGTGAATGTGGCGGGGTATTTATACGACAAGGAGGCGAAGAAGCAGCTGGCGGCGACGGGGAGGATAAGAAAAACAGGGGAGGAGTTGATTTGTAATGGGACGGTGTGTTTCAAGCTGTCTTTTGTGATTATTACGGCGGTGAGTTTGTTTGGGGCTTTGGTTTCTTTGGTTTTGGTGTTGAGAACGAGGAAGTTTTATAAGAGTGACATTTATCGGAGATTTAGAGAGGCGGAGGAGGCGGAGAAGGCGGAGAAGGCGGAGAAGGAAGACGGCAGTGGGTTCATAAACGGCGGCGATATAGTGGAGGACTCGAAACAGGGGATTAGGCAATGA

mRNA sequence

TCATCACCACAAAAATGGACCCAATCTCTGCAGGGTTCAACACCAAATCCTTCACCCTCAAACTAATCAAAGGGCCATGGTTCATGATGTTCGCGTCCTTCCTTATTATGTCCATGGCCGGCATACCTTACATGTTCGGCCTCTACTCCGGCACCATCAAATCTGTATTGGGTTACGACCAAACCACCCTTAATTTCATCAGCTTCTTCAAAGATGTCGGCACCACCGTCGGTGTCTTGGCCGGCTTAATCAACGAAGTAACCCCACCATGGTCCATTCTTGCCATGGGAGCTCTTCTCAATTTCTTCGGTTACTTCATGATTTGGCTCTCTGTTTCCGAGAAGATCCCCACCCATGTCTGGCTCATGTGCTTATATATCTGTGTCGGAGCCAACGCTACAACGTTTGCTAACACCGGCGCCCTCGTCACCTGCGTTAAGAATTACCCGCAACGGCGCGGCGTCGTCATCGGAATTCTTAAGGGCTACATGGGGTTGAGTGGGGCCATTGTTACGCAGCTCTACCATGCGATTTATGGGAAAGATGAAAAGTCGTTGATTTTGTTGTTGGGTTGGTTGCCGGCGGTGATTTCGTTTATTTTCTTGCCGACGGTGCGGCGGATGAAGGTGGAGCACGAGGAGGATGAGCTGAGGGTTTTTTACAGGTTTTTGTATATCTCGTTGGGGCTGGCCGGGTTTTTGATGTTGATGATAATTTTGCAGCAGAAATTTAGTTTTGGGAGAGGGGAGTACGGTGGCAGTGCGGCGGTGGTTACATTTTTGCTCCTTTTGCCGATTGCGGTTGTTGTTGCTCAGGAATTCAAGGCGTGGCGGCGCTTGAATCAGCCTCCGGTGGTTGAGAATGGGCTTTCCTACACGCCGGGGACAGCGGCGTTGAAGAACACGACACCAATGGCACTCCTCCCGAAGAAACCAAAGGAGACAGAACCAGCTAGCAGCACCAAAATTCAATGGTGGAAAAACGTGTTCAATCCGCCAGAAAGAGGAGAAGATTGGACAATTCTCCAAGCTCTGTTCAGCTTCGACATGTTCCTCCTCTTTCTCGCCACCGCGTGCGGCGTCGGCGGGACACTAACCGCCATCGACAACCTCGGACAAATCGGACAGTCACAAGATTACCCAAAGAAAAGCATAAGCACATTCGTGTCGCTTGTCAGCATATGGAATTACCTCGGCAGAGTAATGGCCGGATTCCTCTCCGAACATTTGCTAATCAAATACAGATTCCCTCGTCCTTCAATGCTCACAATCGTTCTTCTTCTTTCCTGCATCGCCCATCTTCTGATCGCCTTCAACCCGAGCGGCGGCCTCTACATTGCCTCTGTTTTAACCGGATTCTGCTACGGCGCCCAGTGGCCTCTGCTTTTCGCCATCGTCTCAGAGGTTTTTGGGCTAAAATATTACGCGACGCTGTATAATTTCGGGTCAGTGGCGAGCCCTGTGGGGCTTTATTTGCTGAATGTGAATGTGGCGGGGTATTTATACGACAAGGAGGCGAAGAAGCAGCTGGCGGCGACGGGGAGGATAAGAAAAACAGGGGAGGAGTTGATTTGTAATGGGACGGTGTGTTTCAAGCTGTCTTTTGTGATTATTACGGCGGTGAGTTTGTTTGGGGCTTTGGTTTCTTTGGTTTTGGTGTTGAGAACGAGGAAGTTTTATAAGAGTGACATTTATCGGAGATTTAGAGAGGCGGAGGAGGCGGAGAAGGCGGAGAAGGCGGAGAAGGAAGACGGCAGTGGGTTCATAAACGGCGGCGATATAGTGGAGGACTCGAAACAGGGGATTAGGCAATGA

Coding sequence (CDS)

ATGGACCCAATCTCTGCAGGGTTCAACACCAAATCCTTCACCCTCAAACTAATCAAAGGGCCATGGTTCATGATGTTCGCGTCCTTCCTTATTATGTCCATGGCCGGCATACCTTACATGTTCGGCCTCTACTCCGGCACCATCAAATCTGTATTGGGTTACGACCAAACCACCCTTAATTTCATCAGCTTCTTCAAAGATGTCGGCACCACCGTCGGTGTCTTGGCCGGCTTAATCAACGAAGTAACCCCACCATGGTCCATTCTTGCCATGGGAGCTCTTCTCAATTTCTTCGGTTACTTCATGATTTGGCTCTCTGTTTCCGAGAAGATCCCCACCCATGTCTGGCTCATGTGCTTATATATCTGTGTCGGAGCCAACGCTACAACGTTTGCTAACACCGGCGCCCTCGTCACCTGCGTTAAGAATTACCCGCAACGGCGCGGCGTCGTCATCGGAATTCTTAAGGGCTACATGGGGTTGAGTGGGGCCATTGTTACGCAGCTCTACCATGCGATTTATGGGAAAGATGAAAAGTCGTTGATTTTGTTGTTGGGTTGGTTGCCGGCGGTGATTTCGTTTATTTTCTTGCCGACGGTGCGGCGGATGAAGGTGGAGCACGAGGAGGATGAGCTGAGGGTTTTTTACAGGTTTTTGTATATCTCGTTGGGGCTGGCCGGGTTTTTGATGTTGATGATAATTTTGCAGCAGAAATTTAGTTTTGGGAGAGGGGAGTACGGTGGCAGTGCGGCGGTGGTTACATTTTTGCTCCTTTTGCCGATTGCGGTTGTTGTTGCTCAGGAATTCAAGGCGTGGCGGCGCTTGAATCAGCCTCCGGTGGTTGAGAATGGGCTTTCCTACACGCCGGGGACAGCGGCGTTGAAGAACACGACACCAATGGCACTCCTCCCGAAGAAACCAAAGGAGACAGAACCAGCTAGCAGCACCAAAATTCAATGGTGGAAAAACGTGTTCAATCCGCCAGAAAGAGGAGAAGATTGGACAATTCTCCAAGCTCTGTTCAGCTTCGACATGTTCCTCCTCTTTCTCGCCACCGCGTGCGGCGTCGGCGGGACACTAACCGCCATCGACAACCTCGGACAAATCGGACAGTCACAAGATTACCCAAAGAAAAGCATAAGCACATTCGTGTCGCTTGTCAGCATATGGAATTACCTCGGCAGAGTAATGGCCGGATTCCTCTCCGAACATTTGCTAATCAAATACAGATTCCCTCGTCCTTCAATGCTCACAATCGTTCTTCTTCTTTCCTGCATCGCCCATCTTCTGATCGCCTTCAACCCGAGCGGCGGCCTCTACATTGCCTCTGTTTTAACCGGATTCTGCTACGGCGCCCAGTGGCCTCTGCTTTTCGCCATCGTCTCAGAGGTTTTTGGGCTAAAATATTACGCGACGCTGTATAATTTCGGGTCAGTGGCGAGCCCTGTGGGGCTTTATTTGCTGAATGTGAATGTGGCGGGGTATTTATACGACAAGGAGGCGAAGAAGCAGCTGGCGGCGACGGGGAGGATAAGAAAAACAGGGGAGGAGTTGATTTGTAATGGGACGGTGTGTTTCAAGCTGTCTTTTGTGATTATTACGGCGGTGAGTTTGTTTGGGGCTTTGGTTTCTTTGGTTTTGGTGTTGAGAACGAGGAAGTTTTATAAGAGTGACATTTATCGGAGATTTAGAGAGGCGGAGGAGGCGGAGAAGGCGGAGAAGGCGGAGAAGGAAGACGGCAGTGGGTTCATAAACGGCGGCGATATAGTGGAGGACTCGAAACAGGGGATTAGGCAATGA

Protein sequence

MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLNFISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCLYICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFSFGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPMALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVASPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFGALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ
Homology
BLAST of CmaCh04G013900 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.2e-29
Identity = 126/542 (23.25%), Postives = 238/542 (43.91%), Query Frame = 0

Query: 22  WFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLNFISFFKDVGTTVGVLAGLINE 81
           W ++ A+  I +  G  + F  YS  +KSVLG  Q  LN+++   D+G   G  +G+   
Sbjct: 44  WTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIALG 103

Query: 82  VTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCLYICVGANATTFANTGALVTCV 141
             P   +L   A + F GY + WL ++  I     L+ L   +   +  + NT   + C+
Sbjct: 104 YFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGLSICWFNTACFILCI 163

Query: 142 KNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLGWLPAVISFIFL-PTV 201
           +++P  R + + +   + G+S A+ +  ++AI        +LL   +P V+SF  L P +
Sbjct: 164 RHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYPVL 223

Query: 202 RRMKVEHEED-ELRVFYRFLYISLGLAGFL--MLMIILQQKFSFGRGEYGGSAAVVTFLL 261
            +  ++   D + R     ++  L +   +    +++     S  R  + G+  ++ F L
Sbjct: 224 TKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLLVFPL 283

Query: 262 LLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPMALLPKKPKETEPASSTK 321
             P+ V     F         PV+   L++      + N   +     K ++T  +S T 
Sbjct: 284 CAPLLVYARDYFL--------PVINARLNHESSGYVMLNIDEL-----KNQKTSVSSKTG 343

Query: 322 IQWWKNV--FNPPERGEDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQDY 381
            +        N    G++ +    +   + +L ++A  CG    L   +NLGQI QS   
Sbjct: 344 YEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG- 403

Query: 382 PKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIVLLLSCIAHLLIAFNP 441
             ++ +T V++ S +++ GR+++    + +  ++R  R     I LL + IA  L+A + 
Sbjct: 404 --QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSS 463

Query: 442 S--GGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVASPVGLYLLNVNVA 501
           S    L  A+ L G   G  +    +I S++FG       +N      P+G  LL   +A
Sbjct: 464 SQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIG-SLLYGYIA 523

Query: 502 GYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFGALVSLVLVLRTRK 556
             +Y+  A   +          + ++C G  C+  +FV    +S+ G + SL L +RT+ 
Sbjct: 524 ASIYEANASPDITPI-----VSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKP 562

BLAST of CmaCh04G013900 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.2e-08
Identity = 57/234 (24.36%), Postives = 109/234 (46.58%), Query Frame = 0

Query: 331 GEDWTILQALFSFDMFLLFLATACGVGGTLTAI-DNLGQIGQS---QDYPKKSISTFVSL 390
           G +   L+  F+     LFL     +GG      +N+G I  +   ++    S ST VSL
Sbjct: 259 GHEGATLKEFFTDKTAWLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSL 318

Query: 391 VSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIVLLLSCIAHLLI------AFNPSGGLY 450
            + ++ + R++ GF SE   ++    RP +L+++ L++   HL++       F+ +    
Sbjct: 319 FATFSTVSRLVVGFSSE--AMESHVSRPVLLSVIALVAACIHLMVPSGIFTVFDNAKYFS 378

Query: 451 IASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVASPVGLYLLNVNVAGYLYDKE 510
           + +++ GF YG+ + L+  IV++V+G+    T++         G ++L + V    Y   
Sbjct: 379 VVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIW---------GSFILALAVGSLGYGLL 438

Query: 511 AKKQLAATGRIRKTGEELICNGTVCFKLSFVII-TAVSLFGALVSLVLVLRTRK 554
             K   A   +       +C+G  C+ L+FVI  T ++   A V  + V   +K
Sbjct: 439 FAKVYDAASEVGVGSMSQVCSGVHCYGLTFVITGTGLAFAAAAVFFIWVFMWKK 481

BLAST of CmaCh04G013900 vs. ExPASy TrEMBL
Match: A0A6J1IJ13 (uncharacterized protein LOC111476469 OS=Cucurbita maxima OX=3661 GN=LOC111476469 PE=4 SV=1)

HSP 1 Score: 1179.1 bits (3049), Expect = 0.0e+00
Identity = 599/599 (100.00%), Postives = 599/599 (100.00%), Query Frame = 0

Query: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60
           MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN
Sbjct: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60

Query: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120
           FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL
Sbjct: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120

Query: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180
           YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS
Sbjct: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180

Query: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240
           LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS
Sbjct: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240

Query: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300
           FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM
Sbjct: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300

Query: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360
           ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL
Sbjct: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360

Query: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420
           TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV
Sbjct: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420

Query: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480
           LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA
Sbjct: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480

Query: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540
           SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG
Sbjct: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540

Query: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 600
           ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ
Sbjct: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 599

BLAST of CmaCh04G013900 vs. ExPASy TrEMBL
Match: A0A6J1GWM6 (uncharacterized protein LOC111458253 OS=Cucurbita moschata OX=3662 GN=LOC111458253 PE=4 SV=1)

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 594/599 (99.17%), Postives = 596/599 (99.50%), Query Frame = 0

Query: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60
           MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN
Sbjct: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60

Query: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120
           FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL
Sbjct: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120

Query: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180
           YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS
Sbjct: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180

Query: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240
           LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS
Sbjct: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240

Query: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300
           FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGT ALKNTTPM
Sbjct: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTPALKNTTPM 300

Query: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360
           ALLPKKPKETEPASSTKIQWWKNVF+PPERGEDWTILQALFS DMFLLFLATACGVGGTL
Sbjct: 301 ALLPKKPKETEPASSTKIQWWKNVFSPPERGEDWTILQALFSVDMFLLFLATACGVGGTL 360

Query: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420
           TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV
Sbjct: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420

Query: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480
           LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA
Sbjct: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480

Query: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540
           SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG
Sbjct: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540

Query: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 600
           ALVSLVLV+RTRKFYKSDIYRRFREAEEAEKAEKAEKEDG GFINGGDIVEDSKQGIRQ
Sbjct: 541 ALVSLVLVMRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGGGFINGGDIVEDSKQGIRQ 599

BLAST of CmaCh04G013900 vs. ExPASy TrEMBL
Match: A0A6J1GZR6 (uncharacterized protein LOC111458711 OS=Cucurbita moschata OX=3662 GN=LOC111458711 PE=4 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 576/599 (96.16%), Postives = 586/599 (97.83%), Query Frame = 0

Query: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60
           MDPISA FNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN
Sbjct: 1   MDPISAKFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60

Query: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120
           FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL
Sbjct: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120

Query: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180
           YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS
Sbjct: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180

Query: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240
           LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS
Sbjct: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240

Query: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300
           FGRGEYGGSAA VT LLLLPI VVVAQEFKAWRRLN+PPVVENGLSYTPGT ALKNTTPM
Sbjct: 241 FGRGEYGGSAAAVTLLLLLPIVVVVAQEFKAWRRLNKPPVVENGLSYTPGTPALKNTTPM 300

Query: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360
           ALLPKKPKETEPASSTKIQWWKNVF+PPERGEDWTILQALFS DMFLLFLATACGVGGTL
Sbjct: 301 ALLPKKPKETEPASSTKIQWWKNVFSPPERGEDWTILQALFSVDMFLLFLATACGVGGTL 360

Query: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420
           TAIDNLGQIGQSQDYPKKSISTFV+LVSIWNYLGRVMAGFLSEHLLIKYRFPR SMLTIV
Sbjct: 361 TAIDNLGQIGQSQDYPKKSISTFVTLVSIWNYLGRVMAGFLSEHLLIKYRFPRTSMLTIV 420

Query: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480
           LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAI+SE+FGLKYYATLYNFGSVA
Sbjct: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIISEIFGLKYYATLYNFGSVA 480

Query: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540
           SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEEL CNGTVCFKLSF+IITAV LFG
Sbjct: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELNCNGTVCFKLSFMIITAVCLFG 540

Query: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 600
           ALVSLVLV+RTRKFYKSDIYRRFREAEE   AEKAEKEDG GFINGGDIV+DSKQG+R+
Sbjct: 541 ALVSLVLVVRTRKFYKSDIYRRFREAEE---AEKAEKEDGGGFINGGDIVKDSKQGLRK 596

BLAST of CmaCh04G013900 vs. ExPASy TrEMBL
Match: A0A1S3BFH1 (uncharacterized protein LOC103489434 OS=Cucumis melo OX=3656 GN=LOC103489434 PE=4 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.7e-300
Identity = 527/597 (88.27%), Postives = 563/597 (94.30%), Query Frame = 0

Query: 7   GFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLNFISFFK 66
           GF+TKSFTLKLIKG WFMMFASFLIMSMAGIPYMFGLYS TIK+VLGYDQTTLNFISFFK
Sbjct: 3   GFSTKSFTLKLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKTVLGYDQTTLNFISFFK 62

Query: 67  DVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCLYICVGA 126
           DVGTTVGV+AGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCLYICVGA
Sbjct: 63  DVGTTVGVVAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCLYICVGA 122

Query: 127 NATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLG 186
           NATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLG
Sbjct: 123 NATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLG 182

Query: 187 WLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFSFGRGEY 246
           WLPA +S +FLPTVRRMKVEHEEDEL+VFYRFLYISLGLAGFLM+MIILQQKFSF RGEY
Sbjct: 183 WLPAAVSLVFLPTVRRMKVEHEEDELKVFYRFLYISLGLAGFLMIMIILQQKFSFDRGEY 242

Query: 247 GGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPMALLPK- 306
           GGSAAVVTFLLLLPIAVVVAQEFK+WRRLN+P  +ENG+S +PG+  LKNTTPM+LLPK 
Sbjct: 243 GGSAAVVTFLLLLPIAVVVAQEFKSWRRLNKPAAIENGISPSPGSPPLKNTTPMSLLPKK 302

Query: 307 -KPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTLTAID 366
            KP++ +P    K +WWKNVFNPP RG+DWTILQALFSFDMFLLFLATACGVGGTLTAID
Sbjct: 303 QKPQQQQP---IKTEWWKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAID 362

Query: 367 NLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIVLLLS 426
           NLGQIGQSQ YPKKSISTFVSLVSIWNYLGRVMAGFLSEHLL+KY+FPRP MLTIVLLLS
Sbjct: 363 NLGQIGQSQHYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLVKYKFPRPLMLTIVLLLS 422

Query: 427 CIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVASPVG 486
           CIAHLLIAFNPSGGLYIAS+LTGFCYGAQWPLLFAIVSE+FGLKYYATLYNFGSVASPVG
Sbjct: 423 CIAHLLIAFNPSGGLYIASILTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVG 482

Query: 487 LYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFGALVS 546
           LYLLNVNVAGYLYDKEAKKQLA  GRIRKTGEEL+CNGTVCFKLSFVIITAVSLFGALVS
Sbjct: 483 LYLLNVNVAGYLYDKEAKKQLAMAGRIRKTGEELVCNGTVCFKLSFVIITAVSLFGALVS 542

Query: 547 LVLVLRTRKFYKSDIYRRFREAEE-AEKAEKAEKEDGSGFIN-GGDIVEDSKQGIRQ 600
           LVLVLRT+KFYKSDIY++F+EAEE AE+ E+ E +DG    N GG ++E++K G+RQ
Sbjct: 543 LVLVLRTKKFYKSDIYKKFKEAEEAAEEVEEEENDDGRVVRNGGGGLMEETKHGLRQ 596

BLAST of CmaCh04G013900 vs. ExPASy TrEMBL
Match: A0A5D3CDI2 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002380 PE=4 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 6.4e-284
Identity = 504/597 (84.42%), Postives = 539/597 (90.28%), Query Frame = 0

Query: 7   GFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLNFISFFK 66
           GF+TKSFTLKLIKG WFMMFASFLIMSMAGIPYMFGLYS TIK+VLGYDQTTLNFISFFK
Sbjct: 3   GFSTKSFTLKLIKGSWFMMFASFLIMSMAGIPYMFGLYSSTIKTVLGYDQTTLNFISFFK 62

Query: 67  DVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCLYICVGA 126
           DVGTTVGV+AGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCLYICVGA
Sbjct: 63  DVGTTVGVVAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCLYICVGA 122

Query: 127 NATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLG 186
           NATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLG
Sbjct: 123 NATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLG 182

Query: 187 WLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFSFGRGEY 246
           WLPA +S +FLPTVRRMKVEHEEDEL+                        KFSF RGEY
Sbjct: 183 WLPAAVSLVFLPTVRRMKVEHEEDELK------------------------KFSFDRGEY 242

Query: 247 GGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPMALLPK- 306
           GGSAAVVTFLLLLPIAVVVAQEFK+WRRLN+P  +ENG+S +PG+  LKNTTPM+LLPK 
Sbjct: 243 GGSAAVVTFLLLLPIAVVVAQEFKSWRRLNKPAAIENGISPSPGSPPLKNTTPMSLLPKK 302

Query: 307 -KPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTLTAID 366
            KP++ +P    K +WWKNVFNPP RG+DWTILQALFSFDMFLLFLATACGVGGTLTAID
Sbjct: 303 QKPQQQQP---IKTEWWKNVFNPPPRGDDWTILQALFSFDMFLLFLATACGVGGTLTAID 362

Query: 367 NLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIVLLLS 426
           NLGQIGQSQ YPKKSISTFVSLVSIWNYLGRVMAGFLSEHLL+KY+FPRP MLTIVLLLS
Sbjct: 363 NLGQIGQSQHYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLVKYKFPRPLMLTIVLLLS 422

Query: 427 CIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVASPVG 486
           CIAHLLIAFNPSGGLYIAS+LTGFCYGAQWPLLFAIVSE+FGLKYYATLYNFGSVASPVG
Sbjct: 423 CIAHLLIAFNPSGGLYIASILTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVG 482

Query: 487 LYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFGALVS 546
           LYLLNVNVAGYLYDKEAKKQLA  GRIRKTGEEL+CNGTVCFKLSFVIITAVSLFGALVS
Sbjct: 483 LYLLNVNVAGYLYDKEAKKQLAMAGRIRKTGEELVCNGTVCFKLSFVIITAVSLFGALVS 542

Query: 547 LVLVLRTRKFYKSDIYRRFREAEE-AEKAEKAEKEDGSGFIN-GGDIVEDSKQGIRQ 600
           LVLVLRT+KFYKSDIY++F+EAEE AE+ E+ E +DG    N GG ++E++K G+RQ
Sbjct: 543 LVLVLRTKKFYKSDIYKKFKEAEEAAEEVEEEENDDGRVVRNGGGGLMEETKHGLRQ 572

BLAST of CmaCh04G013900 vs. NCBI nr
Match: XP_022976285.1 (uncharacterized protein LOC111476469 [Cucurbita maxima])

HSP 1 Score: 1179.1 bits (3049), Expect = 0.0e+00
Identity = 599/599 (100.00%), Postives = 599/599 (100.00%), Query Frame = 0

Query: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60
           MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN
Sbjct: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60

Query: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120
           FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL
Sbjct: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120

Query: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180
           YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS
Sbjct: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180

Query: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240
           LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS
Sbjct: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240

Query: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300
           FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM
Sbjct: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300

Query: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360
           ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL
Sbjct: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360

Query: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420
           TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV
Sbjct: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420

Query: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480
           LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA
Sbjct: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480

Query: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540
           SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG
Sbjct: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540

Query: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 600
           ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ
Sbjct: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 599

BLAST of CmaCh04G013900 vs. NCBI nr
Match: XP_022956542.1 (uncharacterized protein LOC111458253 [Cucurbita moschata])

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 594/599 (99.17%), Postives = 596/599 (99.50%), Query Frame = 0

Query: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60
           MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN
Sbjct: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60

Query: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120
           FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL
Sbjct: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120

Query: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180
           YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS
Sbjct: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180

Query: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240
           LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS
Sbjct: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240

Query: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300
           FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGT ALKNTTPM
Sbjct: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTPALKNTTPM 300

Query: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360
           ALLPKKPKETEPASSTKIQWWKNVF+PPERGEDWTILQALFS DMFLLFLATACGVGGTL
Sbjct: 301 ALLPKKPKETEPASSTKIQWWKNVFSPPERGEDWTILQALFSVDMFLLFLATACGVGGTL 360

Query: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420
           TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV
Sbjct: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420

Query: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480
           LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA
Sbjct: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480

Query: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540
           SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG
Sbjct: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540

Query: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 600
           ALVSLVLV+RTRKFYKSDIYRRFREAEEAEKAEKAEKEDG GFINGGDIVEDSKQGIRQ
Sbjct: 541 ALVSLVLVMRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGGGFINGGDIVEDSKQGIRQ 599

BLAST of CmaCh04G013900 vs. NCBI nr
Match: KAG6601186.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 591/599 (98.66%), Postives = 594/599 (99.17%), Query Frame = 0

Query: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60
           MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN
Sbjct: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60

Query: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120
           FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL
Sbjct: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120

Query: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180
           YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS
Sbjct: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180

Query: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240
           LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS
Sbjct: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240

Query: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300
           FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLN+PPVVENGLSYTPGT ALKNTTPM
Sbjct: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNKPPVVENGLSYTPGTPALKNTTPM 300

Query: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360
           ALLPKKPKETEP SSTKIQWWKNVF+PPERGEDWTILQALFS DMFLLFLATACGVGGTL
Sbjct: 301 ALLPKKPKETEPESSTKIQWWKNVFSPPERGEDWTILQALFSVDMFLLFLATACGVGGTL 360

Query: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420
           TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPR SMLTIV
Sbjct: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRTSMLTIV 420

Query: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480
           LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA
Sbjct: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480

Query: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540
           SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG
Sbjct: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540

Query: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 600
           ALVSLVLV+RTRKFYKSDIYRRFREAEEAEKAEKAEKEDG GFINGGDIVEDSKQGIRQ
Sbjct: 541 ALVSLVLVMRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGGGFINGGDIVEDSKQGIRQ 599

BLAST of CmaCh04G013900 vs. NCBI nr
Match: XP_023535773.1 (uncharacterized protein LOC111797103 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 585/599 (97.66%), Postives = 589/599 (98.33%), Query Frame = 0

Query: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60
           MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN
Sbjct: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60

Query: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120
           FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL
Sbjct: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120

Query: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180
           YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS
Sbjct: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180

Query: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240
           LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS
Sbjct: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240

Query: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300
           FGRGEYGGSAAVVT LLLLPI VVVAQEFKAWRRLN+PPV+ENGLS  PGT ALKNTTPM
Sbjct: 241 FGRGEYGGSAAVVTLLLLLPIVVVVAQEFKAWRRLNKPPVIENGLSSKPGTPALKNTTPM 300

Query: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360
           ALLPKKPKETEPASSTKIQWWKNVF+PPERGEDWTILQALFS DMFLLFLATACGVGGTL
Sbjct: 301 ALLPKKPKETEPASSTKIQWWKNVFSPPERGEDWTILQALFSVDMFLLFLATACGVGGTL 360

Query: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420
           TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV
Sbjct: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420

Query: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480
           LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA
Sbjct: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480

Query: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540
           SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG
Sbjct: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540

Query: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 600
           ALVSLVLV+RTRKFYKSDIYRRFREAEE   AEKAEKEDG GFINGGDIVEDSKQGIRQ
Sbjct: 541 ALVSLVLVMRTRKFYKSDIYRRFREAEE---AEKAEKEDGGGFINGGDIVEDSKQGIRQ 596

BLAST of CmaCh04G013900 vs. NCBI nr
Match: XP_022957273.1 (uncharacterized protein LOC111458711 [Cucurbita moschata])

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 576/599 (96.16%), Postives = 586/599 (97.83%), Query Frame = 0

Query: 1   MDPISAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60
           MDPISA FNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN
Sbjct: 1   MDPISAKFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLN 60

Query: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120
           FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL
Sbjct: 61  FISFFKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIPTHVWLMCL 120

Query: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180
           YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS
Sbjct: 121 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 180

Query: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240
           LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS
Sbjct: 181 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 240

Query: 241 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 300
           FGRGEYGGSAA VT LLLLPI VVVAQEFKAWRRLN+PPVVENGLSYTPGT ALKNTTPM
Sbjct: 241 FGRGEYGGSAAAVTLLLLLPIVVVVAQEFKAWRRLNKPPVVENGLSYTPGTPALKNTTPM 300

Query: 301 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 360
           ALLPKKPKETEPASSTKIQWWKNVF+PPERGEDWTILQALFS DMFLLFLATACGVGGTL
Sbjct: 301 ALLPKKPKETEPASSTKIQWWKNVFSPPERGEDWTILQALFSVDMFLLFLATACGVGGTL 360

Query: 361 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 420
           TAIDNLGQIGQSQDYPKKSISTFV+LVSIWNYLGRVMAGFLSEHLLIKYRFPR SMLTIV
Sbjct: 361 TAIDNLGQIGQSQDYPKKSISTFVTLVSIWNYLGRVMAGFLSEHLLIKYRFPRTSMLTIV 420

Query: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 480
           LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAI+SE+FGLKYYATLYNFGSVA
Sbjct: 421 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIISEIFGLKYYATLYNFGSVA 480

Query: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 540
           SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEEL CNGTVCFKLSF+IITAV LFG
Sbjct: 481 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELNCNGTVCFKLSFMIITAVCLFG 540

Query: 541 ALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKAEKEDGSGFINGGDIVEDSKQGIRQ 600
           ALVSLVLV+RTRKFYKSDIYRRFREAEE   AEKAEKEDG GFINGGDIV+DSKQG+R+
Sbjct: 541 ALVSLVLVVRTRKFYKSDIYRRFREAEE---AEKAEKEDGGGFINGGDIVKDSKQGLRK 596

BLAST of CmaCh04G013900 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 731.5 bits (1887), Expect = 5.5e-211
Identity = 374/586 (63.82%), Postives = 452/586 (77.13%), Query Frame = 0

Query: 5   SAGFNTKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLNFISF 64
           S G + KS T++++ G WFM F S LIMS AG  YMFG+YSG IK  LGYDQTTLN +SF
Sbjct: 5   SPGGSMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSF 64

Query: 65  FKDVGTTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKI-PTHVWLMCLYIC 124
           FKD+G  VGVLAGL+NEVTPPW IL +GA+LNFFGYFMIWL+V+E+I    VW MCLYIC
Sbjct: 65  FKDLGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYIC 124

Query: 125 VGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLIL 184
           VGAN+ +FANTG+LVTCVKN+P+ RGVV+GILKGY+GLSGAI+TQLY A YG+D K LIL
Sbjct: 125 VGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELIL 184

Query: 185 LLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFSFGR 244
           ++GWLPA++SF FL T+R MKV+ + +EL+VFY FLYISLGLA FLM++II+ +   F +
Sbjct: 185 MIGWLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQ 244

Query: 245 GEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPMALL 304
            E+GGSAAVV  LLLLPI VV+ +E K W+                   AL +  P+ ++
Sbjct: 245 SEFGGSAAVVIVLLLLPIIVVILEEKKLWKEKQ---------------VALNDPAPINVV 304

Query: 305 PKKP-------KETEPASSTKI-------QWWKNVFNPPERGEDWTILQALFSFDMFLLF 364
            +KP       K+ +   S ++         W  VFNPPERG+D+TILQALFS DM +LF
Sbjct: 305 TEKPKLDSSEFKDDDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILF 364

Query: 365 LATACGVGGTLTAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKY 424
           LAT CGVGGTLTAIDNLGQIG S  YPK+S+STFVSLVSIWNY GRV++G +SE  LIKY
Sbjct: 365 LATICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKY 424

Query: 425 RFPRPSMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKY 484
           +FPRP MLT+VLLLSC  HLLIAFN  GGLY+ASV+ GFC+GAQWPLLFAI+SE+FGLKY
Sbjct: 425 KFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKY 484

Query: 485 YATLYNFGSVASPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLS 544
           Y+TLYNFGSVASP+G YLLNV VAGYLYD EA KQ  A G+ R  G++L C GT CFKLS
Sbjct: 485 YSTLYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLS 544

Query: 545 FVIITAVSLFGALVSLVLVLRTRKFYKSDIYRRFREAEEAEKAEKA 576
           F+II AV+LFG LVS+VLV+RT+KFYKSDIY++FRE   A + E A
Sbjct: 545 FIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFREKALAAEMEMA 575

BLAST of CmaCh04G013900 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 625.2 bits (1611), Expect = 5.6e-179
Identity = 322/563 (57.19%), Postives = 408/563 (72.47%), Query Frame = 0

Query: 10  TKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLNFISFFKDVG 69
           T  F +    G WFM+FASFLIM+ AG  Y+FG YS  IKS LGYDQTTLN + FFKD+G
Sbjct: 6   TVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLG 65

Query: 70  TTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKI-PTHVWLMCLYICVGANA 129
             VGVL+GLI EVTP W +L +G+ +NF GYFMIWL+V+ K+    VW MCLYIC+GAN+
Sbjct: 66  ANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANS 125

Query: 130 TTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLGWL 189
             FANTGALVTCVKN+P+ RGV++G+LKGY+GLSGAI TQLY AIYG D KSLILL+ WL
Sbjct: 126 QNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWL 185

Query: 190 PAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFSFGRGEYGG 249
           PA +S +F+  +R  KV  + +EL VFY+FLYIS+ LA FLM M I +++  F +  Y  
Sbjct: 186 PAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAA 245

Query: 250 SAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALK-NTTPMALLPKKP 309
           SA +   LL +P+ V V QE + W  +  P    + +        L  +    A +  + 
Sbjct: 246 SATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEE 305

Query: 310 KETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTLTAIDNLG 369
           KET+   ST       VF+PP RGED+TILQAL S DM +LF+AT CG+G +LTA+DNLG
Sbjct: 306 KETKSCFST-------VFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLG 365

Query: 370 QIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIVLLLSCIA 429
           QIG+S  YP  ++S+FVSLVSIWNY GRV +GF+SE+LL KY+ PRP M+T+VLLLSC  
Sbjct: 366 QIGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAG 425

Query: 430 HLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVASPVGLYL 489
           HLLIAF   G +YIAS+L GF +GAQ PLLFAI+SE+FGLKYY+TL+N G +ASP+G Y+
Sbjct: 426 HLLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYI 485

Query: 490 LNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFGALVSLVL 549
           LNV V G LYDKEA KQL A G  RK  ++L C G+ C+KL F+I+ AV+ FGALVSL L
Sbjct: 486 LNVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGL 545

Query: 550 VLRTRKFYKSDIYRRFREAEEAE 571
            +RTR+FYK DIY++FRE+ E+E
Sbjct: 546 AIRTREFYKGDIYKKFRESPESE 561

BLAST of CmaCh04G013900 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 3.3e-91
Identity = 208/565 (36.81%), Postives = 316/565 (55.93%), Query Frame = 0

Query: 15  LKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLNFISFFKDVGTTVGV 74
           +++++  W  M AS  I   +G  Y FG+YS  +KS   YDQ+TL+ +S FKD+G   GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 75  LAGL----------------INEVTPPWSILAMGALLNFFGYFMIWLSVSEKI-PTHVWL 134
            +GL                I     PW +LA+GA+  F GYF+IW SV+  I    V L
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 135 MCLYICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKD 194
           MCL++ + A + TF NT  +V+ V+N+    G  +GI+KG++GLSGAI+ QLY  +   D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 195 EKSLILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQ 254
             S ILLL   P V+S + +P VR  +    +D+ +       +SL +A +LM++IIL  
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYETSVADDK-KHLNGLSAVSLIIAAYLMIIIIL-- 240

Query: 255 KFSFGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNT 314
           K +FG   +     +V  L++L + +++A+  +A R   +  V  +   Y+P  ++ K T
Sbjct: 241 KNTFGLSSWANIVTLVCLLVMLALPLLIAR--RAQRDGMEKTVPHD---YSPLISSPKAT 300

Query: 315 TPMALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVG 374
           T      +   + E   S                E+  +LQA+     +LLFLA  CG+G
Sbjct: 301 TSGNQSSEGDSKVEAGLS----------------ENLNLLQAMKKLSFWLLFLAMICGMG 360

Query: 375 GTLTAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSML 434
             L+ I+N+ QIG+S  Y    I++ VSL SIWN+LGR  AG+ S+ LL K  +PRP ++
Sbjct: 361 SGLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLM 420

Query: 435 TIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFG 494
              L    I HL+IA    G LY+ SV+ G CYG+QW L+  I SE+FG+++  T++N  
Sbjct: 421 AATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTI 480

Query: 495 SVASPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVS 554
           SVASP+G Y+ +V + GY+YDK A            +GE   C G+ CF+LSF+I+ +V+
Sbjct: 481 SVASPIGSYIFSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVA 529

Query: 555 LFGALVSLVLVLRTRKFYKSDIYRR 563
            FG LV++VL  RT+  Y+  + +R
Sbjct: 541 FFGFLVAIVLFFRTKTLYRQILVKR 529

BLAST of CmaCh04G013900 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 2.9e-87
Identity = 202/580 (34.83%), Postives = 316/580 (54.48%), Query Frame = 0

Query: 10  TKSFTLKLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLNFISFFKDVG 69
           T+   +  I   W +  A+  I S AGI Y+FG  S  IKS L Y+Q  L+ +   KD+G
Sbjct: 5   TREKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLG 64

Query: 70  TTVGVLAGLINEVTPPWSILAMGALLNFFGYFMIWLSVSEKIP-THVWLMCLYICVGANA 129
            +VG +AG ++E+ P W+ L +GA+ N  GY  +WL V+ + P   +W MC+ I VG N 
Sbjct: 65  DSVGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNG 124

Query: 130 TTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKSLILLLGWL 189
            T+ NTGALV+ V+N+P+ RG V+GILKG+ GL GAI++Q+Y  I+  +  SLIL++   
Sbjct: 125 ETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVT 184

Query: 190 PAVI----SFIFLPTVRRMKVEHEEDELRVFYRFLY-ISLGLAGFLMLMIILQQKFSFGR 249
           PAV+     F   P     ++   +      + F+Y + L LA +LM ++++Q       
Sbjct: 185 PAVVVVCLMFFIRPVGGHKQIRPTDG---ASFTFIYGVCLLLAAYLMSVMLIQDLVVVSH 244

Query: 250 GEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQP------PVVENGLSYTPGTAALKNT 309
                   V+  +L++PI V +   F  +   N+P      P+V       PG       
Sbjct: 245 NVITVFTIVLFVILVVPILVPIMTSF--FTETNEPDDTIEEPLVPKREDQEPGL-----Q 304

Query: 310 TPMALL----PKKPKETE--PASS-------TKIQWWK---------NVFNPPERGEDWT 369
           TP  +L     +KPK+ +  PAS         + Q  +         N    P RGED+T
Sbjct: 305 TPDLILSEVEDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFT 364

Query: 370 ILQALFSFDMFLLFLATACGVGGTLTAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGR 429
           + QAL   D +L+F +   G G  LT IDNLGQ+ QS  Y    +   VS++SIWN+LGR
Sbjct: 365 LTQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGR 424

Query: 430 VMAGFLSEHLLIKYRFPRPSMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWP 489
           +  G+ SE ++  Y +PRP  + +  L+  + H+  A+   G +YI ++L G  YGA W 
Sbjct: 425 IGGGYFSELVVRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWA 484

Query: 490 LLFAIVSEVFGLKYYATLYNFGSVASPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTG 549
           ++ A  SE+FGLK +  LYNF ++A+P G  + +  +A  +YD+EA++Q  A G +    
Sbjct: 485 IVPATASELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPD 544

Query: 550 EELICNGTVCFKLSFVIITAVSLFGALVSLVLVLRTRKFY 556
           + L CNG++CF L+ +I++   +   ++S++LV RT+  Y
Sbjct: 545 DALRCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVY 570

BLAST of CmaCh04G013900 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 8.5e-87
Identity = 197/556 (35.43%), Postives = 305/556 (54.86%), Query Frame = 0

Query: 16  KLIKGPWFMMFASFLIMSMAGIPYMFGLYSGTIKSVLGYDQTTLNFISFFKDVGTTVGVL 75
           ++++  W  M AS  I   AG  Y FG+YS  +KS   YDQ+TL+ +S FKD+G  VGVL
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 76  AGLINEVTP--------------PWSILAMGALLNFFGYFMIWLSVSEKIP-THVWLMCL 135
           +GL+                   PW ++ +GA+LNF GYF++W SV+  I    V +MCL
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 136 YICVGANATTFANTGALVTCVKNYPQRRGVVIGILKGYMGLSGAIVTQLYHAIYGKDEKS 195
           ++ + A + TF NT  +V+ ++N+    G  +GI+KG++GLSGA++ QLY  +   D K+
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 196 LILLLGWLPAVISFIFLPTVRRMKVEHEEDELRVFYRFLYISLGLAGFLMLMIILQQKFS 255
            ILLL  +P+++S + +P VR  K     DE +       +SL +A +LM+ IIL+   S
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYKTS-TVDEKKHLDGLSTLSLIIAAYLMITIILKSTLS 243

Query: 256 FGRGEYGGSAAVVTFLLLLPIAVVVAQEFKAWRRLNQPPVVENGLSYTPGTAALKNTTPM 315
                   + AV+  LL  P+ V V    +A R   + P+      Y+P    L+ TT  
Sbjct: 244 LPSWANAVTLAVLLVLLSSPLLVAV----RAHRDSIEKPL---SSVYSPLVDNLEATTSG 303

Query: 316 ALLPKKPKETEPASSTKIQWWKNVFNPPERGEDWTILQALFSFDMFLLFLATACGVGGTL 375
            +L                         +  +   +LQA+ + D +LLFLA  CG+G  +
Sbjct: 304 EILML-----------------------DEDKSLNLLQAMCNVDFWLLFLAMICGMGSGI 363

Query: 376 TAIDNLGQIGQSQDYPKKSISTFVSLVSIWNYLGRVMAGFLSEHLLIKYRFPRPSMLTIV 435
           + I+N+ QIG+S  Y    I++ ++L +IWN++GR   G++S+ LL +  +PRP ++   
Sbjct: 364 STINNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATT 423

Query: 436 LLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEVFGLKYYATLYNFGSVA 495
           L    I HL+IA    G LY  S++ G CYG+QW L+  I SE+FG+K+  T+YN  S+A
Sbjct: 424 LGTMTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIA 483

Query: 496 SPVGLYLLNVNVAGYLYDKEAKKQLAATGRIRKTGEELICNGTVCFKLSFVIITAVSLFG 555
           SP+G Y+ +V + GY+YD+               GE   C G  CF+L++V+I +V+  G
Sbjct: 484 SPMGSYIFSVRLIGYIYDRTI------------IGEGNTCYGPHCFRLAYVVIASVAFLG 516

Query: 556 ALVSLVLVLRTRKFYK 557
            LVS VLV RT+  Y+
Sbjct: 544 FLVSCVLVFRTKTIYR 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E11.2e-2923.25Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q6CGU81.2e-0824.36Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28... [more]
Match NameE-valueIdentityDescription
A0A6J1IJ130.0e+00100.00uncharacterized protein LOC111476469 OS=Cucurbita maxima OX=3661 GN=LOC111476469... [more]
A0A6J1GWM60.0e+0099.17uncharacterized protein LOC111458253 OS=Cucurbita moschata OX=3662 GN=LOC1114582... [more]
A0A6J1GZR60.0e+0096.16uncharacterized protein LOC111458711 OS=Cucurbita moschata OX=3662 GN=LOC1114587... [more]
A0A1S3BFH11.7e-30088.27uncharacterized protein LOC103489434 OS=Cucumis melo OX=3656 GN=LOC103489434 PE=... [more]
A0A5D3CDI26.4e-28484.42Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
XP_022976285.10.0e+00100.00uncharacterized protein LOC111476469 [Cucurbita maxima][more]
XP_022956542.10.0e+0099.17uncharacterized protein LOC111458253 [Cucurbita moschata][more]
KAG6601186.10.0e+0098.66Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023535773.10.0e+0097.66uncharacterized protein LOC111797103 [Cucurbita pepo subsp. pepo][more]
XP_022957273.10.0e+0096.16uncharacterized protein LOC111458711 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G39210.15.5e-21163.82Major facilitator superfamily protein [more]
AT2G28120.15.6e-17957.19Major facilitator superfamily protein [more]
AT1G74780.13.3e-9136.81Nodulin-like / Major Facilitator Superfamily protein [more]
AT5G14120.12.9e-8734.83Major facilitator superfamily protein [more]
AT1G18940.18.5e-8735.43Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 21..265
e-value: 8.7E-88
score: 294.0
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 333..556
e-value: 2.0E-11
score: 45.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 12..207
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 220..556
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 345..563
e-value: 1.3E-12
score: 47.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 570..599
NoneNo IPR availablePANTHERPTHR21576:SF122MFS TRANSPORTERcoord: 9..577
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 9..577
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 20..541
e-value: 5.71298E-110
score: 333.064

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G013900.1CmaCh04G013900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity