CmaCh04G012650 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G012650
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	GRAM domain-containing protein
Location	Cma_Chr04: 6471746 .. 6472707 (-)
RNA-Seq Expression	CmaCh04G012650
Synteny	CmaCh04G012650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTAGACATCTTGGAAGAAAAGTTGACAGATTCGTTAATGGAGTCTTTGAACGTGGTAAACTGCGATGTTTTCTTTTTCTCTTGATTTGAGAGAGAATCTCTCTCTGAATTTTACTTTCTCTCCTACATGATTATGATGATGGATTGGATCGTCCTCAAAATTTCCATTTCTAGACTTGTTTTCATGCAGGTGCGAATCGCAAAGTTGCTTATATGTTCATCTAATAAGCATAATTCAACCGGTTAAGCAAGAAAAGAAGGATCACAAAGCTTGTAGAACGCTAAAACCAAAAACTATCGCACTACTATTTGTTTCTCTATAAACAAAGATTGGCATTTTCGTTTTTCTTGTGTGCTAACGTATAACTCAAAAACCAAACTCGATTCAAATGATTACTTGCTTGATGGTTACAACGGAATGATGTCGTGCAGTTAAACTGTCACCGAACATCACCGAGAATTTGAAGGGAAAGCTGTGGTCTGGGGCAAGACTTGTTCAGTTTGGAGGAAATGAGAAGATTTTTCAGAAAATGTTCAATCTCAGCCAAGGAGAGAAGTTACTAAGTTGTTCACGCTGTTATTTGTCAACCACAGCAGGTCCCGTAGCAGGCCTCCTCTTTATCTCCACCCATGTGGTTGCCTTCTGCAGTGACAGACCAATCGTCGTCTCTTCTTCAGAAGGAGAACTGGGCAGATTGAACTACAAGGTAACATTATACATCCTTCAACATTCACAAACCCCTGTGGTTGCTAACAAGTTTTTTGTACTGTGCTTGATTCATCAGGTTATGATCCCATTGAAGAAGATGAAGAGAGTTAACCAAATAGAGCATATAAACAATCCGTCAAAGAAGTACATTCAAGTGGTGACTGTGGATGATTTTGATTTCTGGTTTATGGGTTTTCTGAATTATGAGAAAGCATTCAAGTTCCTCCAAAAGAAGGTTTCTAGAGATTGA

mRNA sequence

ATGTTTAGACATCTTGGAAGAAAAGTTGACAGATTCGTTAATGGAGTCTTTGAACGTGTTAAACTGTCACCGAACATCACCGAGAATTTGAAGGGAAAGCTGTGGTCTGGGGCAAGACTTGTTCAGTTTGGAGGAAATGAGAAGATTTTTCAGAAAATGTTCAATCTCAGCCAAGGAGAGAAGTTACTAAGTTGTTCACGCTGTTATTTGTCAACCACAGCAGGTCCCGTAGCAGGCCTCCTCTTTATCTCCACCCATGTGGTTGCCTTCTGCAGTGACAGACCAATCGTCGTCTCTTCTTCAGAAGGAGAACTGGGCAGATTGAACTACAAGGTTATGATCCCATTGAAGAAGATGAAGAGAGTTAACCAAATAGAGCATATAAACAATCCGTCAAAGAAGTACATTCAAGTGGTGACTGTGGATGATTTTGATTTCTGGTTTATGGGTTTTCTGAATTATGAGAAAGCATTCAAGTTCCTCCAAAAGAAGGTTTCTAGAGATTGA

Coding sequence (CDS)

ATGTTTAGACATCTTGGAAGAAAAGTTGACAGATTCGTTAATGGAGTCTTTGAACGTGTTAAACTGTCACCGAACATCACCGAGAATTTGAAGGGAAAGCTGTGGTCTGGGGCAAGACTTGTTCAGTTTGGAGGAAATGAGAAGATTTTTCAGAAAATGTTCAATCTCAGCCAAGGAGAGAAGTTACTAAGTTGTTCACGCTGTTATTTGTCAACCACAGCAGGTCCCGTAGCAGGCCTCCTCTTTATCTCCACCCATGTGGTTGCCTTCTGCAGTGACAGACCAATCGTCGTCTCTTCTTCAGAAGGAGAACTGGGCAGATTGAACTACAAGGTTATGATCCCATTGAAGAAGATGAAGAGAGTTAACCAAATAGAGCATATAAACAATCCGTCAAAGAAGTACATTCAAGTGGTGACTGTGGATGATTTTGATTTCTGGTTTATGGGTTTTCTGAATTATGAGAAAGCATTCAAGTTCCTCCAAAAGAAGGTTTCTAGAGATTGA

Protein sequence

MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD

Homology

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BLAST of CmaCh04G012650 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 6.2e-48
Identity = 91/160 (56.88%), Postives = 124/160 (77.50%), Query Frame = 0


Query: 7   RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 66
           +K D F NGV ++ K+ P +TE +K KL  GAR++Q GG EKIF+++F +S+GEKL    
Sbjct: 54  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 113

Query: 67  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMKRVNQIE 126
           +CYLSTTAGP+AGLLFIS+  +AFCS+R I V S +G++ R++YKV IPL K+ RVNQ +
Sbjct: 114 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQ 173

Query: 127 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 167
           +   PS+KY++VVTVD FDFWFMGFL+Y+KAF  L+K +S
Sbjct: 174 NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALS 213

[Close]

BLAST of CmaCh04G012650 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 6.9e-47
Identity = 90/160 (56.25%), Postives = 122/160 (76.25%), Query Frame = 0


Query: 7   RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 66
           +K D F NGV ++ KL P +TE +K KL  GAR++Q GG EKI++++F +S  EKL    
Sbjct: 55  KKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAY 114

Query: 67  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMKRVNQIE 126
           +CYLSTTAGP+AGLLFIS+  +AFCS+R I V+S +GEL R++YKV IPL K+  VNQ +
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQ 174

Query: 127 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 167
           +   PS+KY++VVTVD FDFWFMGFL+Y+KAF  L++ +S
Sbjct: 175 NTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALS 214

[Close]

BLAST of CmaCh04G012650 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-45
Identity = 84/162 (51.85%), Postives = 121/162 (74.69%), Query Frame = 0


Query: 7   RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 66
           +K D F NG  ++ KL P +TE +K KL  GA+++Q GG EKI++++F +   EKL    
Sbjct: 55  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 114

Query: 67  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMKRVNQIE 126
           +CYLSTTAGP+AGLLFIS+  +AFCS+R I V+S +G L R++YKV IPL K+  VNQ +
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 174

Query: 127 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           +   PS+KY+++VT+D+FDFWFMGF++Y+KAF  L+K ++ D
Sbjct: 175 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNND 216

[Close]

BLAST of CmaCh04G012650 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 7.1e-44
Identity = 82/162 (50.62%), Postives = 120/162 (74.07%), Query Frame = 0


Query: 7   RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 66
           +K D F NG  ++ KL P +TE +K KL  GA+++Q GG EKI++++F +   EKL    
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 67  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMKRVNQIE 126
           +CYLSTT G +AGLLFIS+  +AFCS+R I V+S +G+L R++YKV IPL K+  VNQ +
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 127 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           +   PS++Y++VVTVD++DFWFMGF++Y+KAF  L+K ++ D
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNED 208

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BLAST of CmaCh04G012650 vs. ExPASy Swiss-Prot
Match: Q9LYV6 (GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.0e-30
Identity = 67/166 (40.36%), Postives = 96/166 (57.83%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           MF    RK +     ++  +K  P+++E   GK+   A+ +  GG E +F+++F     E
Sbjct: 96  MFHTWSRKAETVARNLWHNLKTGPSMSETAWGKVNLTAKAITKGGFESLFRQIFGTEPNE 155

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
            L     CYLSTT GPVAG +++S   VAFCSDRP+  ++  G+     Y+V++PL  + 
Sbjct: 156 TLKKTFACYLSTTTGPVAGTVYLSNARVAFCSDRPLYFTAPSGQESWSYYRVVVPLANVA 215

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 167
            VN +     P +KYIQ+ TVD  DFWFMGF+NYEKA   L   VS
Sbjct: 216 TVNPVVVKETPPEKYIQLTTVDGHDFWFMGFVNYEKATHHLLTSVS 261

[Close]

BLAST of CmaCh04G012650 vs. ExPASy TrEMBL
Match: A0A6J1JHW6 (GEM-like protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111487132 PE=3 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.4e-74
Identity = 149/175 (85.14%), Postives = 149/175 (85.14%), Query Frame = 0


Query: 20  VKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPVAG 79
           VKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPVAG
Sbjct: 7   VKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPVAG 66

Query: 80  LLFISTHVVAFCSDRPIVVSSSEGELGRLNYK--------------------------VM 139
           LLFISTHVVAFCSDRPIVVSSSEGELGRLNYK                          VM
Sbjct: 67  LLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVTLYILQHSQTPVVANKFFVLCLIHQVM 126

Query: 140 IPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           IPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD
Sbjct: 127 IPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 181

[Close]

BLAST of CmaCh04G012650 vs. ExPASy TrEMBL
Match: A0A6J1CD83 (putative GEM-like protein 8 OS=Momordica charantia OX=3673 GN=LOC111010135 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.3e-69
Identity = 129/168 (76.79%), Postives = 146/168 (86.90%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           M R LGRK D FVNG+ + VKLSPN+ E +KGKLW  A+LVQFGG+ KIFQK+FNL QG+
Sbjct: 4   MIRKLGRKADSFVNGILDHVKLSPNVAETVKGKLWLVAKLVQFGGSGKIFQKLFNLRQGD 63

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
           KLLS S CYLSTTAGP+AGLLFIST+ VAFCSDRPI+++S  GELGR NYKVMIP+KKMK
Sbjct: 64  KLLSSSHCYLSTTAGPIAGLLFISTNAVAFCSDRPIIITSPHGELGRFNYKVMIPVKKMK 123

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           RVNQ E+++NPSKKYIQVVTVDDFDFWFMGFLNY KAFKFLQK VS D
Sbjct: 124 RVNQTENVSNPSKKYIQVVTVDDFDFWFMGFLNYGKAFKFLQKGVSED 171

[Close]

BLAST of CmaCh04G012650 vs. ExPASy TrEMBL
Match: A0A1S3BDR8 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488777 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 4.0e-66
Identity = 122/168 (72.62%), Postives = 143/168 (85.12%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           M R LGRK   FVNG+ E VKLSPNI + +KGKLW G +LVQFGG+EKIF KMFNL QG+
Sbjct: 1   MIRELGRKGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGD 60

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
           KLL+ + CYLSTTAGP+AGL+F+STHVVAFCSDRPI++SS  GE+G++ YKVMIP+ K+K
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVK 120

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           RVNQ  + NNP+KKYIQVVTVDDFDFWFMGFLNYEK FKF+QK VS D
Sbjct: 121 RVNQRNNENNPAKKYIQVVTVDDFDFWFMGFLNYEKTFKFIQKGVSMD 168

[Close]

BLAST of CmaCh04G012650 vs. ExPASy TrEMBL
Match: A0A0A0KPM0 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G577990 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.2e-64
Identity = 117/168 (69.64%), Postives = 142/168 (84.52%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           M R LGR+   FVNG+ E VKLSPNI + +KGKLW G +L+QFGG+E IF KMFNL  G+
Sbjct: 1   MIRELGRRGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLLQFGGSENIFHKMFNLEHGD 60

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
           KLL+ + CYLSTTAGP+AGL+F+STHVVAFCSDRPI++SS  GE+G++ YKVMIP+ K+K
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLIFVSTHVVAFCSDRPIIISSPHGEIGKIFYKVMIPVNKVK 120

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           RVNQ  ++NNP+KKYIQVVTVDDFDFWFMGFLN+EKAFKF+Q  VS D
Sbjct: 121 RVNQRNNVNNPAKKYIQVVTVDDFDFWFMGFLNHEKAFKFIQNGVSVD 168

[Close]

BLAST of CmaCh04G012650 vs. ExPASy TrEMBL
Match: A0A6J1HVV6 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111466633 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.2e-63
Identity = 119/168 (70.83%), Postives = 142/168 (84.52%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           M R++G K  RFVNGVF+ VKLSP++T+ +K KLW GA+LVQ G +EKIF+KMF L Q +
Sbjct: 1   MIRNIGSKAARFVNGVFQHVKLSPSVTKTVKRKLWLGAKLVQIGRSEKIFKKMFKLRQDD 60

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
           KLLS S CYLSTTAGP+AGLLF+STHVVAFCSDRPI+ SS   E  R+NYKVMIP+KKMK
Sbjct: 61  KLLSSSHCYLSTTAGPIAGLLFVSTHVVAFCSDRPIIFSSPHEEQSRMNYKVMIPMKKMK 120

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           RVN+ E++NNPSKKYIQVVTVD F+FWFMGFLNY+ AFKFL K +S+D
Sbjct: 121 RVNESENVNNPSKKYIQVVTVDGFEFWFMGFLNYKIAFKFLPKGLSKD 168

[Close]

BLAST of CmaCh04G012650 vs. NCBI nr
Match: XP_023548915.1 (GEM-like protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 339.0 bits (868), Expect = 2.4e-89
Identity = 166/168 (98.81%), Postives = 167/168 (99.40%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           MFRHL RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE
Sbjct: 19  MFRHLRRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 78

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
           KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIV+SSSEGELGRLNYKVMIPLKKMK
Sbjct: 79  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMK 138

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD
Sbjct: 139 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 186

[Close]

BLAST of CmaCh04G012650 vs. NCBI nr
Match: KAG6601067.1 (GEM-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 337.4 bits (864), Expect = 7.0e-89
Identity = 164/168 (97.62%), Postives = 166/168 (98.81%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           MFRHLGRK+DRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNL QGE
Sbjct: 1   MFRHLGRKIDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLRQGE 60

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
           KL SCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIV+SSSEGELGRLNYKVMIPLKKMK
Sbjct: 61  KLQSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVISSSEGELGRLNYKVMIPLKKMK 120

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD
Sbjct: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 168

[Close]

BLAST of CmaCh04G012650 vs. NCBI nr
Match: XP_022990162.1 (GEM-like protein 4 [Cucurbita maxima])

HSP 1 Score: 288.9 bits (738), Expect = 2.8e-74
Identity = 149/175 (85.14%), Postives = 149/175 (85.14%), Query Frame = 0


Query: 20  VKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPVAG 79
           VKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPVAG
Sbjct: 7   VKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCSRCYLSTTAGPVAG 66

Query: 80  LLFISTHVVAFCSDRPIVVSSSEGELGRLNYK--------------------------VM 139
           LLFISTHVVAFCSDRPIVVSSSEGELGRLNYK                          VM
Sbjct: 67  LLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVTLYILQHSQTPVVANKFFVLCLIHQVM 126

Query: 140 IPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           IPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD
Sbjct: 127 IPLKKMKRVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 181

[Close]

BLAST of CmaCh04G012650 vs. NCBI nr
Match: XP_022139162.1 (putative GEM-like protein 8 [Momordica charantia])

HSP 1 Score: 272.3 bits (695), Expect = 2.8e-69
Identity = 129/168 (76.79%), Postives = 146/168 (86.90%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           M R LGRK D FVNG+ + VKLSPN+ E +KGKLW  A+LVQFGG+ KIFQK+FNL QG+
Sbjct: 4   MIRKLGRKADSFVNGILDHVKLSPNVAETVKGKLWLVAKLVQFGGSGKIFQKLFNLRQGD 63

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
           KLLS S CYLSTTAGP+AGLLFIST+ VAFCSDRPI+++S  GELGR NYKVMIP+KKMK
Sbjct: 64  KLLSSSHCYLSTTAGPIAGLLFISTNAVAFCSDRPIIITSPHGELGRFNYKVMIPVKKMK 123

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           RVNQ E+++NPSKKYIQVVTVDDFDFWFMGFLNY KAFKFLQK VS D
Sbjct: 124 RVNQTENVSNPSKKYIQVVTVDDFDFWFMGFLNYGKAFKFLQKGVSED 171

[Close]

BLAST of CmaCh04G012650 vs. NCBI nr
Match: XP_008445893.1 (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 260.8 bits (665), Expect = 8.3e-66
Identity = 122/168 (72.62%), Postives = 143/168 (85.12%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           M R LGRK   FVNG+ E VKLSPNI + +KGKLW G +LVQFGG+EKIF KMFNL QG+
Sbjct: 1   MIRELGRKGYSFVNGILEHVKLSPNIAKTVKGKLWLGTKLVQFGGSEKIFHKMFNLEQGD 60

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
           KLL+ + CYLSTTAGP+AGL+F+STHVVAFCSDRPI++SS  GE+G++ YKVMIP+ K+K
Sbjct: 61  KLLNSAHCYLSTTAGPIAGLVFVSTHVVAFCSDRPIIISSPHGEVGKVFYKVMIPVNKVK 120

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           RVNQ  + NNP+KKYIQVVTVDDFDFWFMGFLNYEK FKF+QK VS D
Sbjct: 121 RVNQRNNENNPAKKYIQVVTVDDFDFWFMGFLNYEKTFKFIQKGVSMD 168

[Close]

BLAST of CmaCh04G012650 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 191.8 bits (486), Expect = 4.4e-49
Identity = 91/160 (56.88%), Postives = 124/160 (77.50%), Query Frame = 0


Query: 7   RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 66
           +K D F NGV ++ K+ P +TE +K KL  GAR++Q GG EKIF+++F +S+GEKL    
Sbjct: 54  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 113

Query: 67  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMKRVNQIE 126
           +CYLSTTAGP+AGLLFIS+  +AFCS+R I V S +G++ R++YKV IPL K+ RVNQ +
Sbjct: 114 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQ 173

Query: 127 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 167
           +   PS+KY++VVTVD FDFWFMGFL+Y+KAF  L+K +S
Sbjct: 174 NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALS 213

[Close]

BLAST of CmaCh04G012650 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 188.3 bits (477), Expect = 4.9e-48
Identity = 90/160 (56.25%), Postives = 122/160 (76.25%), Query Frame = 0


Query: 7   RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 66
           +K D F NGV ++ KL P +TE +K KL  GAR++Q GG EKI++++F +S  EKL    
Sbjct: 55  KKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAY 114

Query: 67  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMKRVNQIE 126
           +CYLSTTAGP+AGLLFIS+  +AFCS+R I V+S +GEL R++YKV IPL K+  VNQ +
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQ 174

Query: 127 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 167
           +   PS+KY++VVTVD FDFWFMGFL+Y+KAF  L++ +S
Sbjct: 175 NTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALS 214

[Close]

BLAST of CmaCh04G012650 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 184.1 bits (466), Expect = 9.2e-47
Identity = 84/162 (51.85%), Postives = 121/162 (74.69%), Query Frame = 0


Query: 7   RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 66
           +K D F NG  ++ KL P +TE +K KL  GA+++Q GG EKI++++F +   EKL    
Sbjct: 117 KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 176

Query: 67  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMKRVNQIE 126
           +CYLSTTAGP+AGLLFIS+  +AFCS+R I V+S +G L R++YKV IPL K+  VNQ +
Sbjct: 177 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 236

Query: 127 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           +   PS+KY+++VT+D+FDFWFMGF++Y+KAF  L+K ++ D
Sbjct: 237 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNND 278

[Close]

BLAST of CmaCh04G012650 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 178.3 bits (451), Expect = 5.0e-45
Identity = 82/162 (50.62%), Postives = 120/162 (74.07%), Query Frame = 0


Query: 7   RKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGEKLLSCS 66
           +K D F NG  ++ KL P +TE +K KL  GA+++Q GG EKI++++F +   EKL    
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 67  RCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMKRVNQIE 126
           +CYLSTT G +AGLLFIS+  +AFCS+R I V+S +G+L R++YKV IPL K+  VNQ +
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 127 HINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVSRD 169
           +   PS++Y++VVTVD++DFWFMGF++Y+KAF  L+K ++ D
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNED 208

[Close]

BLAST of CmaCh04G012650 vs. TAIR 10
Match: AT5G13200.1 (GRAM domain family protein )

HSP 1 Score: 133.7 bits (335), Expect = 1.4e-31
Identity = 67/166 (40.36%), Postives = 96/166 (57.83%), Query Frame = 0


Query: 1   MFRHLGRKVDRFVNGVFERVKLSPNITENLKGKLWSGARLVQFGGNEKIFQKMFNLSQGE 60
           MF    RK +     ++  +K  P+++E   GK+   A+ +  GG E +F+++F     E
Sbjct: 96  MFHTWSRKAETVARNLWHNLKTGPSMSETAWGKVNLTAKAITKGGFESLFRQIFGTEPNE 155

Query: 61  KLLSCSRCYLSTTAGPVAGLLFISTHVVAFCSDRPIVVSSSEGELGRLNYKVMIPLKKMK 120
            L     CYLSTT GPVAG +++S   VAFCSDRP+  ++  G+     Y+V++PL  + 
Sbjct: 156 TLKKTFACYLSTTTGPVAGTVYLSNARVAFCSDRPLYFTAPSGQESWSYYRVVVPLANVA 215

Query: 121 RVNQIEHINNPSKKYIQVVTVDDFDFWFMGFLNYEKAFKFLQKKVS 167
            VN +     P +KYIQ+ TVD  DFWFMGF+NYEKA   L   VS
Sbjct: 216 TVNPVVVKETPPEKYIQLTTVDGHDFWFMGFVNYEKATHHLLTSVS 261

The following BLAST results are available for this feature:

BLAST of CmaCh04G012650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FTA0	6.2e-48	56.88	GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1	[more]
Q9FMW4	6.9e-47	56.25	Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV...	[more]
Q9FMW6	1.3e-45	51.85	GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2	[more]
Q9FMW5	7.1e-44	50.62	GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1	[more]
Q9LYV6	2.0e-30	40.36	GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1	[more]

BLAST of CmaCh04G012650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JHW6	1.4e-74	85.14	GEM-like protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111487132 PE=3 SV=1	[more]
A0A6J1CD83	1.3e-69	76.79	putative GEM-like protein 8 OS=Momordica charantia OX=3673 GN=LOC111010135 PE=3 ...	[more]
A0A1S3BDR8	4.0e-66	72.62	GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488777 PE=3 SV=1	[more]
A0A0A0KPM0	2.2e-64	69.64	GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G577990 PE=3 S...	[more]
A0A6J1HVV6	3.2e-63	70.83	putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111466633 PE=3 SV=...	[more]

BLAST of CmaCh04G012650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548915.1	2.4e-89	98.81	GEM-like protein 4 [Cucurbita pepo subsp. pepo]	[more]
KAG6601067.1	7.0e-89	97.62	GEM-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022990162.1	2.8e-74	85.14	GEM-like protein 4 [Cucurbita maxima]	[more]
XP_022139162.1	2.8e-69	76.79	putative GEM-like protein 8 [Momordica charantia]	[more]
XP_008445893.1	8.3e-66	72.62	PREDICTED: GEM-like protein 4 [Cucumis melo]	[more]

BLAST of CmaCh04G012650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08350.1	4.4e-49	56.88	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23370.1	4.9e-48	56.25	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23350.1	9.2e-47	51.85	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23360.1	5.0e-45	50.62	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G13200.1	1.4e-31	40.36	GRAM domain family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004182	GRAM domain	SMART	SM00568	gram2001c	coord: 48..126 e-value: 5.6E-17 score: 72.4
IPR004182	GRAM domain	PFAM	PF02893	GRAM	coord: 49..165 e-value: 3.6E-22 score: 78.4
IPR011993	PH-like domain superfamily	GENE3D	2.30.29.30		coord: 46..167 e-value: 8.0E-21 score: 75.9
None	No IPR available	PANTHER	PTHR31969:SF38	GEM-LIKE PROTEIN 4	coord: 3..167
IPR037848	GEM-like protein	PANTHER	PTHR31969	GEM-LIKE PROTEIN 2	coord: 3..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G012650.1	CmaCh04G012650.1	mRNA